Untargeted Metabolomics Analysis Combined With Network Pharmacology Reveals Differences in Chemical Profiles and Activities of Different Processed Products of Clematis chinensis Osbeck

IF 1.7 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-09-25 DOI:10.1002/rcm.10143

Jianlin Ke, Lei Wang, Lili Huang, Chongchong Li, Jingjing Yang, Chao Zhang

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引用次数: 0

Abstract

Rationale

Clematis chinensis Osbeck (WLX), an herbal medicine, has been used to treat diseases for thousand years. In clinic, WLX presents four forms, including unprocessed WLX (SZ), WLX mixed with yellow wine (JB), WLX soaked in yellow wine (JJ), and WLX stir-fried with yellow wine (JC). However, few studies have focused on revealing differences in its composition and activity.

Methods

In this study, metabolomics was used to screen featured metabolites of WLX and its processed products. Subsequently, network pharmacology was applied to reveal the differences in their activity.

Results

The results showed that a total of 310 metabolites were identified, and 11 featured metabolites were selected, such as tracheloside, astilbin, and genistein. Network pharmacology analysis suggested that SZ samples mainly exert its specific therapeutic effects by regulating pain pathways, including glutamatergic synapse, spinocerebellar ataxia, and retrograde endocannabinoid signaling. JB, JJ, and JC groups predominantly exert their effects through pathways, including calcium signaling pathway, estrogen signaling pathway, and cAMP signaling pathway, thereby inhibiting the development of inflammatory and pain transmission.

Conclusion

This study will provide a theoretical basis for clinical application of WLX and its processed products and offer a reference for their quality control.

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结合网络药理学的非靶向代谢组学分析揭示了中国铁线莲不同加工产品的化学特征和活性差异

中国铁线莲（Clematis chinensis Osbeck， WLX）是一种草药，几千年来一直被用来治疗疾病。临床上，黄芪有四种形态，分别为未加工的黄芪（SZ）、黄酒混合的黄芪（JB）、黄酒浸泡的黄芪（JJ）、黄酒炒的黄芪（JC）。然而，很少有研究关注于揭示其组成和活性的差异。方法本研究采用代谢组学方法筛选枸杞及其加工产品的特征代谢物。随后，应用网络药理学来揭示它们的活性差异。结果共鉴定出310种代谢物，筛选出11种具有代表性的代谢物，如风草苷、astilbin、染料木素等。网络药理学分析表明，SZ样品主要通过调节谷氨酸能突触、脊髓小脑共济失调、逆行内源性大麻素信号通路等疼痛通路发挥其特异性治疗作用。JB、JJ和JC组主要通过钙信号通路、雌激素信号通路和cAMP信号通路发挥作用，从而抑制炎症和疼痛传递的发生。结论本研究将为枸杞及其炮制品的临床应用提供理论依据，并为其质量控制提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.