{"title":"结合网络药理学的非靶向代谢组学分析揭示了中国铁线莲不同加工产品的化学特征和活性差异","authors":"Jianlin Ke, Lei Wang, Lili Huang, Chongchong Li, Jingjing Yang, Chao Zhang","doi":"10.1002/rcm.10143","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Rationale</h3>\n \n <p><i>Clematis chinensis</i> Osbeck (WLX), an herbal medicine, has been used to treat diseases for thousand years. In clinic, WLX presents four forms, including unprocessed WLX (SZ), WLX mixed with yellow wine (JB), WLX soaked in yellow wine (JJ), and WLX stir-fried with yellow wine (JC). However, few studies have focused on revealing differences in its composition and activity.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In this study, metabolomics was used to screen featured metabolites of WLX and its processed products. Subsequently, network pharmacology was applied to reveal the differences in their activity.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The results showed that a total of 310 metabolites were identified, and 11 featured metabolites were selected, such as tracheloside, astilbin, and genistein. Network pharmacology analysis suggested that SZ samples mainly exert its specific therapeutic effects by regulating pain pathways, including glutamatergic synapse, spinocerebellar ataxia, and retrograde endocannabinoid signaling. JB, JJ, and JC groups predominantly exert their effects through pathways, including calcium signaling pathway, estrogen signaling pathway, and cAMP signaling pathway, thereby inhibiting the development of inflammatory and pain transmission.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>This study will provide a theoretical basis for clinical application of WLX and its processed products and offer a reference for their quality control.</p>\n </section>\n </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 24","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Untargeted Metabolomics Analysis Combined With Network Pharmacology Reveals Differences in Chemical Profiles and Activities of Different Processed Products of Clematis chinensis Osbeck\",\"authors\":\"Jianlin Ke, Lei Wang, Lili Huang, Chongchong Li, Jingjing Yang, Chao Zhang\",\"doi\":\"10.1002/rcm.10143\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Rationale</h3>\\n \\n <p><i>Clematis chinensis</i> Osbeck (WLX), an herbal medicine, has been used to treat diseases for thousand years. In clinic, WLX presents four forms, including unprocessed WLX (SZ), WLX mixed with yellow wine (JB), WLX soaked in yellow wine (JJ), and WLX stir-fried with yellow wine (JC). However, few studies have focused on revealing differences in its composition and activity.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>In this study, metabolomics was used to screen featured metabolites of WLX and its processed products. Subsequently, network pharmacology was applied to reveal the differences in their activity.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The results showed that a total of 310 metabolites were identified, and 11 featured metabolites were selected, such as tracheloside, astilbin, and genistein. Network pharmacology analysis suggested that SZ samples mainly exert its specific therapeutic effects by regulating pain pathways, including glutamatergic synapse, spinocerebellar ataxia, and retrograde endocannabinoid signaling. JB, JJ, and JC groups predominantly exert their effects through pathways, including calcium signaling pathway, estrogen signaling pathway, and cAMP signaling pathway, thereby inhibiting the development of inflammatory and pain transmission.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>This study will provide a theoretical basis for clinical application of WLX and its processed products and offer a reference for their quality control.</p>\\n </section>\\n </div>\",\"PeriodicalId\":225,\"journal\":{\"name\":\"Rapid Communications in Mass Spectrometry\",\"volume\":\"39 24\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rapid Communications in Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/rcm.10143\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rapid Communications in Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/rcm.10143","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Untargeted Metabolomics Analysis Combined With Network Pharmacology Reveals Differences in Chemical Profiles and Activities of Different Processed Products of Clematis chinensis Osbeck
Rationale
Clematis chinensis Osbeck (WLX), an herbal medicine, has been used to treat diseases for thousand years. In clinic, WLX presents four forms, including unprocessed WLX (SZ), WLX mixed with yellow wine (JB), WLX soaked in yellow wine (JJ), and WLX stir-fried with yellow wine (JC). However, few studies have focused on revealing differences in its composition and activity.
Methods
In this study, metabolomics was used to screen featured metabolites of WLX and its processed products. Subsequently, network pharmacology was applied to reveal the differences in their activity.
Results
The results showed that a total of 310 metabolites were identified, and 11 featured metabolites were selected, such as tracheloside, astilbin, and genistein. Network pharmacology analysis suggested that SZ samples mainly exert its specific therapeutic effects by regulating pain pathways, including glutamatergic synapse, spinocerebellar ataxia, and retrograde endocannabinoid signaling. JB, JJ, and JC groups predominantly exert their effects through pathways, including calcium signaling pathway, estrogen signaling pathway, and cAMP signaling pathway, thereby inhibiting the development of inflammatory and pain transmission.
Conclusion
This study will provide a theoretical basis for clinical application of WLX and its processed products and offer a reference for their quality control.
期刊介绍：
Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
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