{"title":"纳米结构依赖的信号强度在多孔氧化铝膜质谱成像","authors":"Masahiro Kotani, Takashi Yanagishita","doi":"10.1002/rcm.10149","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Rationale</h3>\n \n <p>Matrix-assisted laser desorption/ionization (MALDI) is a widely used analytical technique for measuring high-molecular-weight compounds such as proteins. However, in the low-molecular-weight region, interference peaks derived from the matrix occur. Surface-assisted laser desorption/ionization (SALDI), which is matrix-free, does not generate background noise in the low-molecular-weight region and has the advantages of simple sample preparation and reproducibility. We previously developed an ionization method using an anodic porous alumina membrane (APAM) as a SALDI substrate. In this study, we examined the effects of the surface nanostructural properties of APAMs, such as hole diameter and pitch, on the signal intensity in mass spectrometry (MS) imaging.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>APAMs were fabricated using electrolytes of oxalic, malonic, and malic acids and were evaluated by MS using droplet samples and MS imaging. Droplet samples were applied to the back surface of the APAMs. MS imaging was conducted using 20-μm-thick mouse brain sections to compare the signal-to-noise ratio (SNR) of each APAM.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>APAMs were fabricated with hole diameters (D<sub>h</sub>) of 24–419 nm, interhole distances (D<sub>int</sub>) of 100–625 nm, and open area ratios (OAR) of 5%–46%. From MS and MS imaging results, signal intensity at the same OAR increased in the order of D<sub>int</sub> = 100, 625, and 270 nm, and the condition D<sub>h</sub>/D<sub>int</sub> = 131/270 nm provided the highest SNR. In addition, APAMs with a D<sub>h</sub> of less than 84 nm and an OAR lower than 10% exhibited lower signal intensities.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>We fabricated APAMs under various conditions and identified the processing conditions that provided the highest SNR of SALDI imaging. SALDI imaging using the APAMs fabricated under these high SNR conditions is expected to be applicable in various fields such as materials science and metabolomics, as it does not generate interference peaks in the low-molecular-weight region.</p>\n </section>\n </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nanostructure-Dependent Signal Intensity in Through-Hole Porous Alumina Membranes for Mass Spectrometry Imaging\",\"authors\":\"Masahiro Kotani, Takashi Yanagishita\",\"doi\":\"10.1002/rcm.10149\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Rationale</h3>\\n \\n <p>Matrix-assisted laser desorption/ionization (MALDI) is a widely used analytical technique for measuring high-molecular-weight compounds such as proteins. However, in the low-molecular-weight region, interference peaks derived from the matrix occur. Surface-assisted laser desorption/ionization (SALDI), which is matrix-free, does not generate background noise in the low-molecular-weight region and has the advantages of simple sample preparation and reproducibility. We previously developed an ionization method using an anodic porous alumina membrane (APAM) as a SALDI substrate. In this study, we examined the effects of the surface nanostructural properties of APAMs, such as hole diameter and pitch, on the signal intensity in mass spectrometry (MS) imaging.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>APAMs were fabricated using electrolytes of oxalic, malonic, and malic acids and were evaluated by MS using droplet samples and MS imaging. Droplet samples were applied to the back surface of the APAMs. MS imaging was conducted using 20-μm-thick mouse brain sections to compare the signal-to-noise ratio (SNR) of each APAM.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>APAMs were fabricated with hole diameters (D<sub>h</sub>) of 24–419 nm, interhole distances (D<sub>int</sub>) of 100–625 nm, and open area ratios (OAR) of 5%–46%. From MS and MS imaging results, signal intensity at the same OAR increased in the order of D<sub>int</sub> = 100, 625, and 270 nm, and the condition D<sub>h</sub>/D<sub>int</sub> = 131/270 nm provided the highest SNR. In addition, APAMs with a D<sub>h</sub> of less than 84 nm and an OAR lower than 10% exhibited lower signal intensities.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>We fabricated APAMs under various conditions and identified the processing conditions that provided the highest SNR of SALDI imaging. SALDI imaging using the APAMs fabricated under these high SNR conditions is expected to be applicable in various fields such as materials science and metabolomics, as it does not generate interference peaks in the low-molecular-weight region.</p>\\n </section>\\n </div>\",\"PeriodicalId\":225,\"journal\":{\"name\":\"Rapid Communications in Mass Spectrometry\",\"volume\":\"40 1\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rapid Communications in Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/rcm.10149\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rapid Communications in Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/rcm.10149","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Nanostructure-Dependent Signal Intensity in Through-Hole Porous Alumina Membranes for Mass Spectrometry Imaging
Rationale
Matrix-assisted laser desorption/ionization (MALDI) is a widely used analytical technique for measuring high-molecular-weight compounds such as proteins. However, in the low-molecular-weight region, interference peaks derived from the matrix occur. Surface-assisted laser desorption/ionization (SALDI), which is matrix-free, does not generate background noise in the low-molecular-weight region and has the advantages of simple sample preparation and reproducibility. We previously developed an ionization method using an anodic porous alumina membrane (APAM) as a SALDI substrate. In this study, we examined the effects of the surface nanostructural properties of APAMs, such as hole diameter and pitch, on the signal intensity in mass spectrometry (MS) imaging.
Methods
APAMs were fabricated using electrolytes of oxalic, malonic, and malic acids and were evaluated by MS using droplet samples and MS imaging. Droplet samples were applied to the back surface of the APAMs. MS imaging was conducted using 20-μm-thick mouse brain sections to compare the signal-to-noise ratio (SNR) of each APAM.
Results
APAMs were fabricated with hole diameters (Dh) of 24–419 nm, interhole distances (Dint) of 100–625 nm, and open area ratios (OAR) of 5%–46%. From MS and MS imaging results, signal intensity at the same OAR increased in the order of Dint = 100, 625, and 270 nm, and the condition Dh/Dint = 131/270 nm provided the highest SNR. In addition, APAMs with a Dh of less than 84 nm and an OAR lower than 10% exhibited lower signal intensities.
Conclusions
We fabricated APAMs under various conditions and identified the processing conditions that provided the highest SNR of SALDI imaging. SALDI imaging using the APAMs fabricated under these high SNR conditions is expected to be applicable in various fields such as materials science and metabolomics, as it does not generate interference peaks in the low-molecular-weight region.
期刊介绍：
Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
