Carbohydrate Polymers最新文献

High temperature and humidity storage alter starch properties of faba (Vicia faba) and adzuki beans (Vigna angularis) associated with hard-to-cook quality.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-12-04 DOI: 10.1016/j.carbpol.2024.123119

Dilini Perera, Bin Jia, Lavaraj Devkota, Surya P Bhattarai, Joe Panozzo, Sushil Dhital

{"title":"High temperature and humidity storage alter starch properties of faba (Vicia faba) and adzuki beans (Vigna angularis) associated with hard-to-cook quality.","authors":"Dilini Perera, Bin Jia, Lavaraj Devkota, Surya P Bhattarai, Joe Panozzo, Sushil Dhital","doi":"10.1016/j.carbpol.2024.123119","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.123119","url":null,"abstract":"Hard-to-cook (HTC) beans are characterised by extended cooking times. Although the changes in cell walls limiting hydration in HTC beans are widely investigated, the role of macro-molecules (starch and protein, which constitute >80 % of beans) are almost overlooked. This study investigates the structural changes in starch associated with the HTC quality in faba and adzuki beans stored at contrasting temperature and humidity regimes. Beans were stored at 4 °C (control) and 40 °C with relative humidity (RH) levels of 60 % and 80 %. Significant changes in starch properties were observed, particularly in beans stored at 40 °C and 80 % RH, with swelling power decreasing by 7 % and 12 % for faba and adzuki beans, respectively. Additionally, gelatinisation behaviour was negatively affected, with peak temperatures increasing for adzuki beans (from 66.64 °C to 68.46 °C) and enthalpy rising for faba beans (from 9.25 J/g to 10.64 J/g) along with an increase in relative crystallinity. Overall findings indicate that storage at elevated temperature (40 °C) under both moderate and high humidity conditions (60 % and 80 %) primarily or partially leads to developing HTC beans due to molecular rearrangement of starch at helical and crystalline levels.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"123119"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hijacking the hyaluronan assisted iron endocytosis to promote the ferroptosis in anticancer photodynamic therapy.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-12-05 DOI: 10.1016/j.carbpol.2024.123123

Hong Deng, Jiayu Chen, Huimin Wang, Runmeng Liu, Yiyi Zhang, Hui Chang, Ching-Hsuan Tung, Weiqi Zhang

{"title":"Hijacking the hyaluronan assisted iron endocytosis to promote the ferroptosis in anticancer photodynamic therapy.","authors":"Hong Deng, Jiayu Chen, Huimin Wang, Runmeng Liu, Yiyi Zhang, Hui Chang, Ching-Hsuan Tung, Weiqi Zhang","doi":"10.1016/j.carbpol.2024.123123","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.123123","url":null,"abstract":"Photodynamic therapy (PDT) eradicates tumor cells by the light-stimulated reactive oxygen species, which also induces lipid peroxidation (LPO) and subsequently ferroptosis, an iron-depended cell death. Ferroptosis has a tremendous therapeutic potential in cancer treatment, however, the ferroptosis efficiency is largely limited by the available iron in cells. Through hijacking the CD44-mediated iron endocytosis of hyaluronan (HA), here PDT with enhanced ferroptosis was realized by a HA@Ce6 nanogel self-assembled from HA, a photosensitizer Chlorin e6 (Ce6) and Fe3+ as cross-linkers. Taking advantages of HA's natural affinity towards CD44, HA@Ce6 enabled a targeted Ce6 delivery in CD44-overexpressed breast cancer cells and meanwhile enhanced iron uptake to \"fuel\" ferroptosis together with the light-stimulated LPO. Further, HA@Ce6 demonstrated an excellent anticancer PDT efficacy and ferroptosis induction in the murine 4 T1 xenograft model. This HA@Ce6 successfully exploited the role of HA in iron transport to sensitize ferroptosis, providing a potent strategy to facilitate the anticancer PDT.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"123123"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions between cellulose nanocrystals and conventional/gemini surfactants.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-12-10 DOI: 10.1016/j.carbpol.2024.123132

Gaili Cao, Jiaxin Xu, Lian Han, Yi Wang, Weinan Zhao, Xiaoming Zhou, Yebin Lee, Watson Loh, Kam Chiu Tam

{"title":"Interactions between cellulose nanocrystals and conventional/gemini surfactants.","authors":"Gaili Cao, Jiaxin Xu, Lian Han, Yi Wang, Weinan Zhao, Xiaoming Zhou, Yebin Lee, Watson Loh, Kam Chiu Tam","doi":"10.1016/j.carbpol.2024.123132","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.123132","url":null,"abstract":"Research on the interaction between surfactants and cellulose nanocrystals (CNC) has mainly focused on the interaction between CNC and conventional surfactants, and there are no reported studies on the interaction between CNC and gemini surfactants. The interactions between CNC and conventional surfactant (tetradecyltrimethylammonium bromide, termed as TTAB), asymmetric gemini surfactant ([C14H29(CH3)2N+(CH2)6N+(CH3)2C6H13]Br (14-6-6)) or symmetric gemini surfactant ([C14H29(CH3)2N+(CH2)6N+(CH3)2C14H29]Br2 (14-6-14)) were examined. With increasing surfactant concentration, interaction of TTAB/CNC was described by three regions, i.e. electrostatic interaction, CNC induced micellization and dilution of free micelles. However, in the case of gemini surfactant/CNC, four binding regimes were observed, i.e. cooperative adsorption, CNC induced micellization, formation and dilution of free micelles. The behavior of 14-6-6/CNC was similar to 14-6-14/CNC where CNC promoted the partition of gemini surfactant to the air-water interface at high surfactant concentration, while it was inhibited at low surfactant concentration. At low CNC concentration, micellization induced by CNC and aggregation of surfactant/CNC complexes were absent. pH had a minimal impact on the binding process at low CNC concentration, but it affected the binding at higher CNC concentration. Additionally, the presence of electrolytes influenced the micellization process induced by CNC by reducing the electrostatic interactions.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"123132"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NMR spectroscopic studies of chitin oligomers - Resolution of individual residues and characterization of minor amide cis conformations.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-12-05 DOI: 10.1016/j.carbpol.2024.123122

Piera Wiesinger, Gustav Nestor

{"title":"NMR spectroscopic studies of chitin oligomers - Resolution of individual residues and characterization of minor amide cis conformations.","authors":"Piera Wiesinger, Gustav Nestor","doi":"10.1016/j.carbpol.2024.123122","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.123122","url":null,"abstract":"Chitin is the second most abundant biopolymer in nature after cellulose and is composed of N-acetylglucosamine (GlcNAc) connected via β(1 → 4)-glycosidic bonds. Despite its prominence in nature and diverse roles in pharmaceutical and food technological applications, there is still a need to develop methods to study structure and function of chitin and its corresponding oligomers. Efforts have been made to analyse chitin oligomers by NMR spectroscopy, but spectral overlap has prevented any differentiation between the interior residues. In this study, chitin oligomers up to hexaose with natural abundance of 15N were analysed with NMR spectroscopy in aqueous solution. Different 1H,15N-HSQC pulse sequences were evaluated to obtain the best resolution and sensitivity. Interior residues were resolved in the 15N dimension and detailed chemical shifts of amide proton and nitrogen are reported for the first time. Additionally, all oligomers were analysed for the presence of the amide cis form and its corresponding chemical shifts were assigned. This study exploits the information that can be obtained from chitin oligomers with NMR spectroscopy and depicts methods for detailed analysis of glycans containing oligomers of N-acetylglucosamine.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"123122"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oligosaccharide-assisted resolution of holothurian fucosylated chondroitin sulfate for fine structure and P-selectin inhibition.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-12-12 DOI: 10.1016/j.carbpol.2024.123145

Ying Pan, Huifang Sun, Xi Gu, Sujuan Li, Shengtao Yang, Liang Zhang, Hui Mao, Pin Wang, Shasha Yang, Ronghua Yin, Zhili Zuo, Jinhua Zhao

{"title":"Oligosaccharide-assisted resolution of holothurian fucosylated chondroitin sulfate for fine structure and P-selectin inhibition.","authors":"Ying Pan, Huifang Sun, Xi Gu, Sujuan Li, Shengtao Yang, Liang Zhang, Hui Mao, Pin Wang, Shasha Yang, Ronghua Yin, Zhili Zuo, Jinhua Zhao","doi":"10.1016/j.carbpol.2024.123145","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.123145","url":null,"abstract":"Fucosylated chondroitin sulfate (FCS) from Holothuria mexicana (FCSHm) was selected for investigation because of its intriguing branch features. Selective β-eliminative depolymerization and the bottom-up assembly were performed to unravel that FCSHm consisted of a {D-GlcA-β1,3-D-GalNAc4S6S} backbone and branches of alternating FucS (55 %) and D-GalNAcS-α1,2-L-FucS (45 %), the highest proportion of disaccharide branch reported to date. In branches, sulfation could occur at every free -OH site except O-3 of GalNAc, being the most complex and various structure features of natural FCS. Detailed structure-activity relationship analyses showed that FCSHm and its depolymerized products (>8 kDa) effectively competed with SLeX and PSGL-1 to bind with P-sel at nano-molar level and the inhibition potency increased with Mw increasing. For the structural trisaccharide unit, di-O-sulfation of the FucS (Fuc2S4S and Fuc3S4S) was almost 10-fold more potent than mono-O-sulfation (Fuc4S). Unexpectedly, higher sulfation of the disaccharide-branched tetrasaccharide unit reduced inhibition. The reversal may attribute to fewer interactions with P-sel by molecular docking study. These results suggested that the specific configuration underpinned the potent inhibition, whereas the size and sulfate number of branches were not the key factors for the specific binding. dHmF4 (8.0 kDa) potently blocked the platelet-leukocyte aggregates formation, further verifying the potential value in use.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"123145"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-induced synergistic changes in color and shape of soft actuator based on degradable carbon dots/sodium alginate gel.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-12-06 DOI: 10.1016/j.carbpol.2024.123112

Yingge Shi, Xuyuan Yang, Yongqiang Zhang, Siyu Lu

引用次数: 0

Polymeric saccharides: Effect on physical characteristics and creaminess perception of non-fat whipped cream analogue.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-11-23 DOI: 10.1016/j.carbpol.2024.123055

Yameng Han, Ling Zhu, Hui Zhang, Tongtong Liu

{"title":"Polymeric saccharides: Effect on physical characteristics and creaminess perception of non-fat whipped cream analogue.","authors":"Yameng Han, Ling Zhu, Hui Zhang, Tongtong Liu","doi":"10.1016/j.carbpol.2024.123055","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.123055","url":null,"abstract":"In this study, the improvement effects of different polymeric saccharides, including native starch, maltodextrin and inulin, replacing 10 % sucrose on the physical characteristics and creaminess perception of non-fat whipped cream system were investigated. Systems containing maltodextrin had more uniform particle size and bubble distribution. This resulted in higher whipping performance and lower friction characteristics. When dextrose equivalent was 39 and 19, the overrun and friction coefficient achieved best (441.69 % and 0.0554), respectively. Conversely, systems containing starch showed better mechanical characteristics. This was achieved by foaming a three-phase foam system containing air, water and gelatinized starch particles. Especially, potato starch showed the highest hardness (2.88 N), storage modulus (1570.39 Pa) and the proportion of immobilized water (96.73 %), as well as the lowest loss tangent (0.13). The rheological and tribological properties after simulated oral processing were significantly correlated with sensory attributes. Systems with low apparent viscosity were negatively correlated with smoothness, but positively correlated with mouth-coating. In contrast, systems with a low friction coefficient (at 1 mm/s) received high scores for creaminess. As a result, systems containing maltodextrin scored higher in creaminess, and also showed strongest perception (4.06) when the dextrose equivalent was 19.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"123055"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simplified process for preparing native and depolymerized capsular polysaccharides of Streptococcus pneumoniae.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-11-27 DOI: 10.1016/j.carbpol.2024.123070

Yuelong Li, Hantian Yao, Yanli Liu, Xueting Huang, Xin Cao, Jianlong Wang, Zhixin Gao, Haifa Zheng, Jing-Ren Zhang, Jiankai Liu

{"title":"Simplified process for preparing native and depolymerized capsular polysaccharides of Streptococcus pneumoniae.","authors":"Yuelong Li, Hantian Yao, Yanli Liu, Xueting Huang, Xin Cao, Jianlong Wang, Zhixin Gao, Haifa Zheng, Jing-Ren Zhang, Jiankai Liu","doi":"10.1016/j.carbpol.2024.123070","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.123070","url":null,"abstract":"Streptococcus pneumoniae is a major pathogen of bacterial pneumonia, meningitis, sepsis, and otitis media. The pathogenicity of this bacterium is largely attributed to its polysaccharide capsule, a protective layer around bacterial cell that enables bacteria to resist against host defense. Capsular polysaccharides (CPSs) of S. pneumoniae have been used as antigens to develop a variety of pneumococcal vaccines against invasive pneumococcal disease (IPD). These vaccines have been proven to be effective in reducing the incidence of IPD cases that are caused by vaccine-covered serotypes at the global scale. A crucial step in the manufacture of pneumococcal polysaccharide and conjugate vaccines is to purify native and depolymerized CPSs to meet strict quality standards in purity and structural integrity. The major impurities comprise proteins, nucleic acids and cell wall polysaccharides (CWPS). Traditionally, the removal of impurities to obtain purified native CPSs involves a complex process of purification, after which purified CPSs need to be further size-reduced to obtain depolymerized CPSs by multi-step approaches. In this study, we streamlined the process of CPS purification, which involves firstly ultrafiltration, followed by one-step acid precipitation, and finally diafiltration to obtain pure native CPSs. Furthermore, hydrolysis using trifluoroacetic acid (TFA) was integrated into the process to obtain purified depolymerized CPSs. The native and depolymerized CPSs produced by this optimized process were comparable to the materials obtained by the traditional approaches in purity and structural integrity, which would meet the quality standards of CPSs for vaccine production in the current edition of the European Pharmacopeia.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"123070"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic fucoidan nanoparticles with regulation of macrophage polarization for targeted therapy of acute lung injury.

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-11-13 DOI: 10.1016/j.carbpol.2024.122967

Weili Yang, Hui Liu, Xiaowei Liu, Yuxin Cao, Xuemei Wang, Xinxin Wang, Wenwen Cao, Chunjing Guo, Daquan Chen

{"title":"Biomimetic fucoidan nanoparticles with regulation of macrophage polarization for targeted therapy of acute lung injury.","authors":"Weili Yang, Hui Liu, Xiaowei Liu, Yuxin Cao, Xuemei Wang, Xinxin Wang, Wenwen Cao, Chunjing Guo, Daquan Chen","doi":"10.1016/j.carbpol.2024.122967","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.122967","url":null,"abstract":"Acute lung injury (ALI) is a complex acute respiratory illness with a high mortality rate. Reactive oxygen species (ROS) play a pivotal role in ALI, inducing cellular damage, inflammation, and oxidative stress, thereby exacerbating the severity of the injury. In this study, inspired by the \"subtractive\" strategy, we developed a fucoidan-based macrophage membrane bio-nanosystem, abbreviated as MF@CB, designed as an anti-inflammatory and antioxidant agent to alleviate lipopolysaccharide (LPS)-induced inflammation in ALI. MF@CB coated with macrophage membrane for effective targeting and accumulation in ALI lesions. In addition, MF@CB activates Nrf2 transcriptional activity in macrophages, inhibiting ROS synthesis at its origin while effectively removing ROS already present in the ALI. This dual-pronged approach demonstrates robust antioxidant properties and restores the macrophage antioxidant defense barrier. In the LPS-induced ALI mouse model, MF@CB significantly mitigated lung inflammatory damage by modulating lung macrophage polarization and inhibiting the over-secretion of pro-inflammatory cytokines by activated immune cells. More importantly, unlike most surface modification strategies because it remove the molecule, this approach is easier to apply and potentially safer and may provide useful insights into the development of more effective therapeutic strategies for ALI.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"122967"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn².

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-01 Epub Date: 2024-11-30 DOI: 10.1016/j.carbpol.2024.123069

Nicola Filippo Virzì, Patricia Diaz-Rodriguez, Angel Concheiro, Ana Otero, Antonino Mazzaglia, Valeria Pittalà, Carmen Alvarez-Lorenzo

{"title":"Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2.","authors":"Nicola Filippo Virzì, Patricia Diaz-Rodriguez, Angel Concheiro, Ana Otero, Antonino Mazzaglia, Valeria Pittalà, Carmen Alvarez-Lorenzo","doi":"10.1016/j.carbpol.2024.123069","DOIUrl":"https://doi.org/10.1016/j.carbpol.2024.123069","url":null,"abstract":"Biofilm formation on biological and material surfaces represents a heavy health and economic burden for both patient and society. To contrast this phenomenon, medical devices combining antibacterial and pro-wound healing abilities are a promising strategy. In the present work, Xanthan gum/Guar gum (XG/GG)-based scaffolds were tuned with thymol and Zn2+ to obtain wound dressings that combine antibacterial and antibiofilm properties and favour the healing process. The tuning process preserved the 3D extrusion-based printability of the XG/GG ink. Scaffolds swelling profile was assessed in PBS pH 7.4, and the resistance to compressive forces was studied using a texturometer. The scaffolds microarchitectures were analyzed by SEM, while ATR-FTIR spotlighted the chemical modifications of the customized materials. Thymol and Zn2+ release was analyzed in biologically relevant media, showing a burst release in the first hours. The antibacterial properties were confirmed against S. aureus, P. aeruginosa, and S. epidermidis by isothermal microcalorimetry and biofilm viable cell counting. Incorporation of hydroxypropyl-β-cyclodextrin (HPβCD) improved thymol loading (7- and 14-times higher thymol content) and enhanced the antimicrobial and antioxidant performances of the dressing, while the presence of Zn2+ strongly potentiated the antimicrobial activity, showing a potent antibiofilm response in both Gram-positive and Gram-negative strains of clinical concern. The thymol and Zn2+ combination led to a reduction of 99.95 %, 99.99 %, and 98.26 %, of biofilm formation against S. aureus, P. aeruginosa, and S. epidermidis, respectively. Furthermore, the scaffolds demonstrated good hemocompatibility, cytocompatibility, tissue integration and pro-angiogenic features in an in ovo CAM model.","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"123069"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0