Carbohydrate Polymers最新文献_第10页

Understanding the application-related features of sweet potato starch varying in multi-scale supramolecular structure

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-19 DOI: 10.1016/j.carbpol.2024.122997

Yabin Guo , Dongling Qiao , Siming Zhao , Xiaowen Pi , Bowen Li , Kai Zhang , Binjia Zhang

{"title":"Understanding the application-related features of sweet potato starch varying in multi-scale supramolecular structure","authors":"Yabin Guo , Dongling Qiao , Siming Zhao , Xiaowen Pi , Bowen Li , Kai Zhang , Binjia Zhang","doi":"10.1016/j.carbpol.2024.122997","DOIUrl":"10.1016/j.carbpol.2024.122997","url":null,"abstract":"<div><div>Understanding the application-related features of sweet potato starch (SPS) is necessary for its utilization, which remains limited. Here, seven starches isolated from different sweet potato varieties (HA, SS, YSA, YSB, YSC, YSD, and YSE) were used. It was confirmed that the multi-scale structure possesses significant effects upon the application-related features of SPS. Relatively thinner crystalline lamellae contributed to less resistance to hydrothermal effects and thus increased peak viscosity (<em>η</em><sub>p</sub>) value, while thicker crystalline lamellae and smaller granule size resulted in elevated paste stability under shearing. The synergism of amylose content and molecular orders on digestion rate (<em>k</em>) was also observed, and a higher proportion of stable molecular orders and high thermal stability resulted in an attenuated <em>k</em>. Consequently, the YSA starch with the highest proportion (ca. 0.63) of stable long-range molecular orders (indicated by the high melting temperature: ca. 63 °C) within the starch granule could restrict enzymes' diffusion and permeation towards the starch matrices, and thus elevated resistant starch (79.66 %), possessing huge potential for designing low glycaemic index foods. Additionally, the higher amylose content of the HA starch (15.14 %) and the YSB starch (15.31 %) may contribute to the amylose aggregation and the amylopectin recrystallization, and thus increased gel strength.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"350 ","pages":"Article 122997"},"PeriodicalIF":10.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A micrometer sized porous β-cyclodextrin polymer for improving bioavailability of poorly soluble drug 用于提高难溶性药物生物利用度的微米级多孔 β-环糊精聚合物

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-19 DOI: 10.1016/j.carbpol.2024.123042

Sisi Yi, Tao Guo, Yan Wang, Xiaodi Yang, Yue Liao, Xiyu Tang, Xiangjun Zhang

引用次数: 0

Reinforced enzyme mineralized chitosan hydrogels with superior mechanical and osteogenic properties 具有卓越机械和成骨特性的强化酶矿化壳聚糖水凝胶

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-19 DOI: 10.1016/j.carbpol.2024.123032

Yao Wang , Nihui Zhang , Junwei Zhang, Ruijuan Yao, Jing He, Fang Wu

{"title":"Reinforced enzyme mineralized chitosan hydrogels with superior mechanical and osteogenic properties","authors":"Yao Wang , Nihui Zhang , Junwei Zhang, Ruijuan Yao, Jing He, Fang Wu","doi":"10.1016/j.carbpol.2024.123032","DOIUrl":"10.1016/j.carbpol.2024.123032","url":null,"abstract":"<div><div>As a natural cationic polymer material, the application of chitosan hydrogel for bone tissue engineering has been greatly limited due to its poor mechanical strength. Enzymatic mineralization has drawn increased attention to effectively improve the mechanical properties of hydrogels. In this study, carboxymethyl chitosan (CMCS) hydrogels cross-linked with different concentrations of genipin (2.5 %, 5 % and 10 %) were prepared and further mineralized through enzyme-induced biomimetic mineralization. The mechanical properties of the CMCS hydrogels were significantly increased as a result of mineralization, showing improvement of 1200–1500 % on storage moduli, and even exhibiting certain tensile behavior with the elongation rate of 30–35 %, likely due to the uniform formation and small size of mineralized products. Interestingly, the cationicity of chitosan also exerted an important modulation effect and the mineralization behavior and mechanical properties of mineralized hydrogels. In addition, the enzymatic mineralized hydrogels showed enhanced biocompatibility and osteogenic differentiation in-vitro, likely due to its superior mechanical properties and the introduction of calcium phosphate biominerals. In vivo experiments further suggest excellent bone-forming activity for the enzymatic mineralized hydrogels. Overall, tuning cationicity and enzymatic mineralization provide an effective approach for the preparation of chitosan hydrogels with superior mechanical and biological properties for bone tissue engineering application.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123032"},"PeriodicalIF":10.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of carrageenan on stability and 3D printing performance of high internal phase pickering emulsion stabilized by soy protein isolate aggregates under neutral condition 卡拉胶对中性条件下用大豆分离蛋白聚合体稳定的高内相萃取乳液的稳定性和 3D 打印性能的影响

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-19 DOI: 10.1016/j.carbpol.2024.123020

Rui Li , Xiuqin Guo , Pengfei Liu , Yuanyuan Li , Si Qiu , Yuntao Wang

{"title":"Effect of carrageenan on stability and 3D printing performance of high internal phase pickering emulsion stabilized by soy protein isolate aggregates under neutral condition","authors":"Rui Li , Xiuqin Guo , Pengfei Liu , Yuanyuan Li , Si Qiu , Yuntao Wang","doi":"10.1016/j.carbpol.2024.123020","DOIUrl":"10.1016/j.carbpol.2024.123020","url":null,"abstract":"<div><div>High internal phase Pickering emulsion (HIPPE) stabilized by heat induced soy protein isolate aggregates (HSPI) alone had limited stability and poor 3D printing performance. While there is few research about HIPPE stabilized by HSPI and polysaccrides at neutral pH condition, where HSPI and ĸ-carrageenan (CG) were combined to fabricate HIPPE in this research. It was found that the incorporation of CG significantly decreased the droplet size and improved the storage stability of the resulting HIPPE. Moreover, the presence of CG improved the freeze-thaw stability of HIPPE after one freeze-thaw cycle. In addition, the addition of CG significantly improved the structural integrity of the 3D printed HIPPE and enhanced the printing precision. This was because the presence of CG decreased the interfacial tension, increased the zeta potential and viscosity of HSPI-CG, thus promoting the adsorption of particles to the oil-water interface more effectively. Moreover, the presence of CG significantly enhanced the viscoelasticity of the resulting HIPPE. These results can be further attributed to the strong hydrogen bonding and hydrophobic interaction between HSPI and CG at neutral pH condition, which can be confirmed from results of Fourier-transform infrared spectroscopy and Isothermal titration calorimeter. So the incorporation of CG endowed HIPPE with more excellent properties at a lower solid particle concentration.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123020"},"PeriodicalIF":10.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring cyclodextrin-driven advancements in aqueous Zn-ion battery: A review 探索环糊精驱动的水性 Zn 离子电池技术进步：综述

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-19 DOI: 10.1016/j.carbpol.2024.123041

Ahmed Hazem Abdelhay, Abdulilah Dawoud Bani-Yaseen

{"title":"Exploring cyclodextrin-driven advancements in aqueous Zn-ion battery: A review","authors":"Ahmed Hazem Abdelhay, Abdulilah Dawoud Bani-Yaseen","doi":"10.1016/j.carbpol.2024.123041","DOIUrl":"10.1016/j.carbpol.2024.123041","url":null,"abstract":"<div><div>The growing demand for sustainable and efficient energy storage solutions has emphasized the value of aqueous zinc-ion batteries (AZIBs) as a safer and less expensive alternative to lithium-ion batteries. Despite their potential, AZIBs confront considerable obstacles, particularly the production of zinc dendrites and side reactions such hydrogen evolution, which limit their cycling stability and practical applicability. In this review, we explore the prospective function of cyclodextrins (CDs), a type of supramolecular carbohydrate polymer, in addressing such challenges. CDs, with their unique capacity to form inclusion complexes, provide an innovative approach for controlling zinc deposition, limiting dendrite development, and minimizing parasitic interactions. We present a detailed assessment of recent improvements in the use of CDs to change electrolytes and the electrode/electrolyte interface, resulting in improved electrochemical performance of AZIBs. This review focuses on CDs' potential as multifunctional additives for improving zinc anode stability, extending battery life, and facilitating large-scale AZIB adoption.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123041"},"PeriodicalIF":10.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing enzymatic post-treatments by endoglucanase (EG) and lytic polysaccharide monooxygenase (LPMO) on microfibrillated cellulose (MFC) to enhance cellulose film fabrication 比较内切葡聚糖酶（EG）和裂解多糖单氧化酶（LPMO）对微纤化纤维素（MFC）的酶后处理，以提高纤维素膜的制造水平

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-18 DOI: 10.1016/j.carbpol.2024.123037

Jie Wu , Yufeng Yuan , Qi Hua , Tao Zou , Zhangmin Wan , Gio Ferson Bautista , Orlando Rojas , Scott Renneckar , Jack Saddler

{"title":"Comparing enzymatic post-treatments by endoglucanase (EG) and lytic polysaccharide monooxygenase (LPMO) on microfibrillated cellulose (MFC) to enhance cellulose film fabrication","authors":"Jie Wu , Yufeng Yuan , Qi Hua , Tao Zou , Zhangmin Wan , Gio Ferson Bautista , Orlando Rojas , Scott Renneckar , Jack Saddler","doi":"10.1016/j.carbpol.2024.123037","DOIUrl":"10.1016/j.carbpol.2024.123037","url":null,"abstract":"<div><div>Cellulose is the world's most abundant natural polymer and it can be used as a substitute for fossil derived products. The work described here evaluated the use of mono-component enzyme treatment, using endoglucanase (EG) and lytic polysaccharide monooxygenase (LPMO), to improve the properties of micro-fibrillated cellulose (MFC) produced from mechanically refined kraft pulp. Endoglucanase treatment of the pulp significantly reduced the degree of polymerization (DP) of the cellulose by promoting fiber cutting. In contrast, LPMO treatment only slightly reduced the fiber length and pulp viscosity. However, the introduction of carboxylic acid groups by the action of LPMO appeared to enhance the accessible surface area of cellulose. Enzyme-treated MFC showed improved nano-fibrillation with transmittance and water retention values increased after both enzyme treatments. The cellulose films derived from enzyme-treated MFC showed enhanced mechanical properties with the LPMO-treated films showing superior tensile strength (77 MPa) and Young's modulus (3600 MPa). Thermogravimetric analysis (TGA) also indicated improved thermal stability for both enzyme-treated films. LPMO treatments proved more effective, promoting nano-fibrillation while maintaining fiber integrity and enhanced intermolecular interactions.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123037"},"PeriodicalIF":10.7,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization of a α-d-glucan from Ginkgo biloba seeds and its protective effects on non-alcoholic fatty liver disease in mice 银杏叶籽中α-d-葡聚糖的结构特征及其对小鼠非酒精性脂肪肝的保护作用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-18 DOI: 10.1016/j.carbpol.2024.123022

Shuxiao Liang, Zhijie Yao, Jinxiang Chen, Jin Qian, Yufeng Dai, Haitao Li

{"title":"Structural characterization of a α-d-glucan from Ginkgo biloba seeds and its protective effects on non-alcoholic fatty liver disease in mice","authors":"Shuxiao Liang, Zhijie Yao, Jinxiang Chen, Jin Qian, Yufeng Dai, Haitao Li","doi":"10.1016/j.carbpol.2024.123022","DOIUrl":"10.1016/j.carbpol.2024.123022","url":null,"abstract":"<div><div>Nonalcoholic fatty liver disease (NAFLD) poses a great global challenge to public health, yet it holds promise for amelioration through plant-derived polysaccharide. <em>Ginkgo biloba</em> seeds have long been used as medicine and food, which has potential benefits for various chronic diseases. However, the protective role of <em>Ginkgo biloba</em> seed polysaccharide against NAFLD remains unclear. In this study, we isolated and purified polysaccharide (GBSP-2) from <em>Ginkgo biloba</em> seeds. GBSP-2 is composed of α-<span>d</span>-glucopyranose residues, which are interconnected with α-<span>d</span>-glucopyranose units linked by (1→4) bonds, (1→4,6) bonds and (1→3,4) bonds, the ratio distribution is 15:1:1. By studying a mouse model, we investigated the effect of GBSP-2 (100 or 200 mg/kg) on high-fat-diet-induced NAFLD. We demonstrated that GBSP-2 significantly alleviated NAFLD, as evidenced by reduced hepatic steatosis, decreased inflammation, improved oxidative stress and ameliorative glucolipid metabolic disorders. Furthermore, GBSP-2 mitigated gut microbiota disturbance of NAFLD mice and markedly increased the abundance of <em>Akkermansia</em>, <em>Romboutsia</em>, <em>Lactobacillus</em> and <em>Bacteroides</em>. Mechanistically, GBSP-2 could activate AMPK/ACC signaling pathway to inhibit lipid synthesis by generating 3,4-dihydroxyphenylpropionic acid (DHPPA). Overall, these findings suggest that GBSP-2 plays a multi-channel and multi-target role in improving NAFLD through the gut-liver axis.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123022"},"PeriodicalIF":10.7,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anisotropic hydrogel sensors with muscle-like structures based on high-absorbent alginate fibers 基于高吸水性藻酸盐纤维的具有肌肉样结构的各向异性水凝胶传感器

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-17 DOI: 10.1016/j.carbpol.2024.123015

Chen Hang, Zihan Guo, Kai Li, Jiuyong Yao, Hailing Shi, Ruihao Ge, Junxuan Liang, Fengyu Quan, Kewei Zhang, Xing Tian, Yanzhi Xia

{"title":"Anisotropic hydrogel sensors with muscle-like structures based on high-absorbent alginate fibers","authors":"Chen Hang, Zihan Guo, Kai Li, Jiuyong Yao, Hailing Shi, Ruihao Ge, Junxuan Liang, Fengyu Quan, Kewei Zhang, Xing Tian, Yanzhi Xia","doi":"10.1016/j.carbpol.2024.123015","DOIUrl":"10.1016/j.carbpol.2024.123015","url":null,"abstract":"<div><div>Hydrogel sensors have attracted much attention as they play a critical role in health monitoring, multifunctional electronic skin, and human-machine interfaces. However, the isotropic structure makes existing hydrogel sensors exhibit isotropic sensing performance. Therefore, it is a challenge to fabricate hydrogels with human tissue-like structures to achieve anisotropic sensing performance. Herein, we proposed a novel method to prepare anisotropic hydrogel sensors using high-absorbent alginate fibers. The anisotropic hydrogel, HAFG@CNTs, was prepared by adsorbing carbon nanotubes on high-absorbent alginate fibers and immobilized using polyacrylamide bonds. The hydrogel had anisotropic mechanical properties and anisotropic ionic conductivity. The modulus and toughness in the parallel fiber direction were 2.31 and 3.75 times higher than those in the perpendicular fiber direction, respectively, and the sensitivity of the parallel fiber direction was higher than that of the vertical direction when strain occurred. In addition, machine learning algorithms were used to predict and classify different action signals obtained from HAFG@CNTs with an accuracy of up to 98.18 %. These advantages offer great potential for applying HAFG@CNTs to wearable devices and medical monitoring.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123015"},"PeriodicalIF":10.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of tunable hyaluronic acid and O′-carboxymethyl chitosan formulations for the minimally invasive delivery of multifunctional therapies targeting rheumatoid arthritis 设计可调透明质酸和 O′-羧甲基壳聚糖制剂，用于微创递送针对类风湿性关节炎的多功能疗法

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-17 DOI: 10.1016/j.carbpol.2024.123018

Daniel Fernández-Villa , Aitor Herraiz , Kyra de Wit , Fernando Herranz , María Rosa Aguilar , Luis Rojo

{"title":"Design of tunable hyaluronic acid and O′-carboxymethyl chitosan formulations for the minimally invasive delivery of multifunctional therapies targeting rheumatoid arthritis","authors":"Daniel Fernández-Villa , Aitor Herraiz , Kyra de Wit , Fernando Herranz , María Rosa Aguilar , Luis Rojo","doi":"10.1016/j.carbpol.2024.123018","DOIUrl":"10.1016/j.carbpol.2024.123018","url":null,"abstract":"<div><div>The development of injectable, dual-component formulations based on natural-based polysaccharides is a promising strategy for the localized treatment of rheumatoid arthritis (RA). In the present study, biomimetic formulations consisting of aldehyde-functionalized hyaluronic acid (AHA) and <em>O</em>-carboxymethyl chitosan (OCC) were developed, presenting rapid in situ gelation rates and finely tunable physicochemical properties. These two properties allowed for the controlled delivery of anti-inflammatory, antioxidant, and pro-regenerative agents (i.e., strontium-methotrexate (SrMTX) and europium-tannic acid nanocomplexes (EuTA NCs), making them suitable for application in in vivo RA-models. Biological analyses demonstrated the system's cytocompatibility and its ability to modulate the activity of human articular chondrocytes at the secretome level and scavenge nitric oxide (NO). Moreover, the loaded cargoes not only extended the anti-inflammatory properties of the formulation but also the radiolabeling of EuTA NCs with <sup>68</sup>Ga allowed the visualization of the gel by positron emission tomography (PET). Overall, this work presents the design and in vitro evaluation of an easily modulable polymeric system that allows the in situ release of a multifunctional therapy with promising perspectives for RA treatment.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123018"},"PeriodicalIF":10.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunostimulatory effects of isomalto/malto-polysaccharides via TLR2 and TLR4 in preventing doxycycline-induced cytokine loss 异麦芽酮/麦芽多糖通过 TLR2 和 TLR4 在防止强力霉素诱导的细胞因子丢失方面的免疫刺激作用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-17 DOI: 10.1016/j.carbpol.2024.122980

Luis Silva-Lagos , Adil Ijaz , P. Buwalda , Sonia Kassai , Cynthia E. Klostermann , Hans Leemhuis , Edwin J.A. Veldhuizen , Henk A. Schols , Gabriel López-Velázquez , Paul de Vos

{"title":"Immunostimulatory effects of isomalto/malto-polysaccharides via TLR2 and TLR4 in preventing doxycycline-induced cytokine loss","authors":"Luis Silva-Lagos , Adil Ijaz , P. Buwalda , Sonia Kassai , Cynthia E. Klostermann , Hans Leemhuis , Edwin J.A. Veldhuizen , Henk A. Schols , Gabriel López-Velázquez , Paul de Vos","doi":"10.1016/j.carbpol.2024.122980","DOIUrl":"10.1016/j.carbpol.2024.122980","url":null,"abstract":"<div><div>Isomalto/malto-polysaccharides (IMMPs) are α-glucans with prebiotic potential used as food ingredients. However, their ability to exert direct cellular effects remains unknown. IMMPs may enhance immunity by activating toll-like receptors (TLRs), key for defense against pathogens. Doxycycline is an antibiotic that requires an effective immune function but paradoxically has immune-attenuating effects by reducing TLR2 activity, potentially increasing antibiotic needs. We hypothesize that IMMPs are recognized by various cell surface TLRs, leading to the activation of the NF-κB signaling pathway. Furthermore, IMMPs' immune-stimulating effect could prevent the doxycycline-induced reduction of TLR2 activity in immune cells. IMMPs activated TLR2, increasing NF-κB signaling by 3.42- and 6.37-fold at 1 and 2 mg/mL, respectively. TLR4 activation increased 5.47-, 7.39-, and 8.34-fold at 0.5, 1, and 2 mg/mL. IMMPs enhanced IL-8, TNFα, and IL1-RA production in THP-1 monocytes. Additionally, preincubation of macrophages with IMMPs enhanced cytokine production and partially prevented doxycycline-induced cytokine reduction in response to TLR2 activation. Molecular docking analyses support IMMPs and doxycycline binding to these TLRs. These findings suggest that IMMPs stimulate immunity via TLR2 and TLR4, partially mitigating doxycycline's adverse effects. This provides a dietary strategy to enhance pathogen clearance, reduce antibiotic needs, and support immune health.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"350 ","pages":"Article 122980"},"PeriodicalIF":10.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0