Carbohydrate Polymers最新文献_第10页

Further understanding the unique structure derived from interaction of soy proteins with acylated starch of various chain-length short-chain fatty acids

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-18 DOI: 10.1016/j.carbpol.2025.123417

Sheng Ke , Anqi Wang , Peng Yu , Xuanyu Wang , Guandi Chen , Yanhong Lin , Chris Blanchard , Zhongkai Zhou , Guohua Zhao

{"title":"Further understanding the unique structure derived from interaction of soy proteins with acylated starch of various chain-length short-chain fatty acids","authors":"Sheng Ke , Anqi Wang , Peng Yu , Xuanyu Wang , Guandi Chen , Yanhong Lin , Chris Blanchard , Zhongkai Zhou , Guohua Zhao","doi":"10.1016/j.carbpol.2025.123417","DOIUrl":"10.1016/j.carbpol.2025.123417","url":null,"abstract":"<div><div>Waxy maize starch was acylated <em>via</em> different chain-length of short-chain fatty acids (SCFAs) (referred to AS, PS and BS, respectively), followed by investigation of their structural characteristics of complexes formed between the acylated starch and soy protein isolate (SPI) <em>via</em> FTIR, NMR, XRD and CLSM, <em>etc.</em> This is the first time to reveal that ζ-potential value of the complexes between two polymers was proportional to chain length of SCFAs. The occurrence of new peak at 1580 cm<sup>−1</sup> in AS-SPI, PS-SPI and BS-SPI might imply the interaction between NH<sub>2</sub> group of SPI and hydroxyl group of acylated starch. Consistently, disappearance of characteristic peaks OH-2, 3 (5.40, 5.50 ppm) from <sup>1</sup>H-shift in NMR further evidenced covalent reactions between two polymers. Interestingly, although acylation reduced crystallinity, new diffraction peaks at 7.5°, 14.5° and 19.8° of WMS-PS and WMS-BS revealed the formation of V-type crystalline structure rather than WMS-AS, indicating the importance of chain length of SCFAs for manipulating complexes structure. Furthermore, G′ of BS-SPI complexes was greater than G″ under various frequency, but not for other types of complexes. Meanwhile, BS-SPI complexes demonstrated a better encapsulation wall for probiotics protection against gastrointestinal digestion, which might indicate association of structural characteristics with functionality.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"356 ","pages":"Article 123417"},"PeriodicalIF":10.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of chitin purity on its dissolution behaviour in alkaline solvent

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-18 DOI: 10.1016/j.carbpol.2025.123414

Julia Pohling , Kelly Hawboldt , Deepika Dave

{"title":"Influence of chitin purity on its dissolution behaviour in alkaline solvent","authors":"Julia Pohling , Kelly Hawboldt , Deepika Dave","doi":"10.1016/j.carbpol.2025.123414","DOIUrl":"10.1016/j.carbpol.2025.123414","url":null,"abstract":"<div><div>The biopolymer chitin is a promising ingredient for bioplastics due to its strength and stability. Chitin fibres must be isolated from biomass by stripping away tightly bound minerals, proteins, and pigments. Depending on the desired chitin purity, this isolation process can be long and cost-prohibitive on industrial scale. Following isolation, chitin fibres must be dissolved or separated which is challenging due to chitins recalcitrance. Cryo-assisted dissolution in potassium hydroxide has been identified as a fast and non-toxic approach. However, to date, this method has not received widespread attention and has not been evaluated for chitins from different species.</div><div>The present study demonstrated the dissolution of Northern pink shrimp (<em>P. borealis</em>) chitin using KOH solvent and the influence of residual impurities on dissolution behaviour was investigated for the first time. Three chitin samples with purities ranging from 84 to 97 % <em>w</em>/w were compared, and results confirmed that chitin with lower purity can be successfully dissolved in KOH solvent, provided that minerals were removed prior to dissolution. However, dissolution behaviour and physical properties were strikingly different between the samples. Microscopic analysis of chitin particles allowed the differentiation between exoskeleton features and the identification of insoluble particles, providing potential new avenues to improve dissolution processes.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"356 ","pages":"Article 123414"},"PeriodicalIF":10.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Agar-gelatin Maillard conjugates used for Pickering emulsion stabilization” [Carbohydrate Polymers 340 (2024) 122293]

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-18 DOI: 10.1016/j.carbpol.2025.123383

Lipeng Du, Yi Ru, Huifen Weng, Yonghui Zhang, Jun Chen, Anfeng Xiao, Qiong Xiao

引用次数: 0

An injectable cyclodextrin extended polyurethane/carboxymethyl cellulose hydrogel for controlled release of insulin: In-vitro and in-vivo diabetic animal model study

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-17 DOI: 10.1016/j.carbpol.2025.123396

Subham Sekhar Mandal, Avishek Mallick Choudhury, Anuradha Gupta, Pralay Maiti

{"title":"An injectable cyclodextrin extended polyurethane/carboxymethyl cellulose hydrogel for controlled release of insulin: In-vitro and in-vivo diabetic animal model study","authors":"Subham Sekhar Mandal, Avishek Mallick Choudhury, Anuradha Gupta, Pralay Maiti","doi":"10.1016/j.carbpol.2025.123396","DOIUrl":"10.1016/j.carbpol.2025.123396","url":null,"abstract":"<div><div>Diabetes is a persistent medical condition causing significant anguish for individuals and imposing substantial financial burdens on healthcare systems globally. The inability of human body to produce adequate insulin as a result of insulin resistance or β-cell loss upsets the glucose homeostasis, causing the blood glucose level to rise suddenly. Diabetic patients must get therapy to maintain their blood glucose levels within normal range, preferably through emulating normal insulin secretion, to reduce consequences such as organ deterioration. In order to preserve natural glucose balance, frequent subcutaneous insulin injections are the standard and the last line of treatment. Nevertheless, frequent and continuous subcutaneous injections are associated with low patient compliance and localized insulin amyloidosis. These issues can be resolved with steady and sustained insulin delivery. In this context, to provide sustained insulin delivery over an extended period of time for regulating blood glucose, an injectable cyclodextrin extended polyurethane (PU-co-CD)/CMC hydrogel has been designed. Due to unique composition of insulin-loaded PU-co-CD copolymer, the carboxy methyl cellulose (CMC) forms an injectable hydrogel. The synthesis and processing of this chain extended copolymer have been validated through spectroscopic, chromatographic, and rheological investigations. In diabetic Swiss albino mice, subcutaneous injection of PU-co-CD encapsulated insulin in a gel medium regulates insulin release for up to 3 days, compared to <12 h with conventional insulin injection. PU-co-CD-gel is a promising drug delivery vehicle due to its biocompatible and biodegradable nature and the formulation demonstrates sustained insulin delivery in diabetic condition without affecting any body organ, as observed through histopathology.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"356 ","pages":"Article 123396"},"PeriodicalIF":10.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alkali-extracted rhamnogalactoarabinan from Chaetomorpha linum: Characterisation and anti-type 2 diabetic effect

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-17 DOI: 10.1016/j.carbpol.2025.123388

Yajing Yang , Xiao Chu , Yan Li , Haining Meng , Ling Qin

{"title":"Alkali-extracted rhamnogalactoarabinan from Chaetomorpha linum: Characterisation and anti-type 2 diabetic effect","authors":"Yajing Yang , Xiao Chu , Yan Li , Haining Meng , Ling Qin","doi":"10.1016/j.carbpol.2025.123388","DOIUrl":"10.1016/j.carbpol.2025.123388","url":null,"abstract":"<div><div>Sulphated galactoarabinans, extracted from members belonging to the Cladophoraceae (Chlorophyceae) family, have been reported to have potential as novel anti-diabetic agents. However, relatively few studies have investigated this research topic. In the present study, a water-soluble polysaccharide designated CBS2 was obtained from <em>Chaetomorpha linum</em> using dilute alkaline solution, preparative anion exchange, and size-exclusion chromatography. Chemical and high-performance liquid chromatography analyses revealed that CBS2 is a sulphated rhamnogalactoarabinan. Gas chromatography-mass spectrometry and nuclear magnetic resonance analyses revealed that the backbone of CBS2 was primarily made up of 4-linked β-L-arabinopyranose, which was sulphated at C-2/C-3. The branches were primarily composed of 4-linked-β-<span>d</span>-galactopyranose and positioned at C-3 of 4-linked β-L-arabinopyranose. Transmission electron microscopy and thioflavin T fluorescence experiments showed that CBS2 successfully suppressed human islet amyloid polypeptide (hIAPP) aggregation in vitro. In NIT-1 cells, CBS2 reduced hIAPP-induced cytotoxicity. CBS2 markedly lowered the production of intracellular reactive oxygen species and oxidative stress induced by hIAPP aggregation in NIT-1 cells. In addition, CBS2 co-localised with mitochondria and protected mitochondrial function from hIAPP aggregation-induced damage by stabilising the mitochondrial membrane potential. These data suggest that CBS2 may potentially serve as an anti-diabetic agent for the treatment of type 2 diabetes mellitus.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"356 ","pages":"Article 123388"},"PeriodicalIF":10.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulating gelatinization, retrogradation, and hydrogel properties of potato starch through calcium chloride-controlled crosslinking and crystallization behavior

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-17 DOI: 10.1016/j.carbpol.2025.123371

Ke Xu , Lili Tan , Haonan Sun , Chuanyu Chong , Lihua Li , Boyang Sun , Zhuojun Yao , Yuting Zhuang , Li Wang

{"title":"Manipulating gelatinization, retrogradation, and hydrogel properties of potato starch through calcium chloride-controlled crosslinking and crystallization behavior","authors":"Ke Xu , Lili Tan , Haonan Sun , Chuanyu Chong , Lihua Li , Boyang Sun , Zhuojun Yao , Yuting Zhuang , Li Wang","doi":"10.1016/j.carbpol.2025.123371","DOIUrl":"10.1016/j.carbpol.2025.123371","url":null,"abstract":"<div><div>Due to the inherent susceptibility of single-polymer starch molecules to retrogradation, the practical application of green starch hydrogels is remarkably limited. Here, we propose a simple strategy to achieve the multifunctionality of starch hydrogels by employing polymer amorphization. Calcium chloride was used to promote the gelatinization of starch granules, disrupting their crystalline structure without the need for heating. Additionally, during the initial stage of hydrogel formation, the effects induced by calcium chloride effectively suppressed starch retrogradation. This suppression induced the formation of uniform aggregates of polymer chains, enabling tunable polymer amorphization and the coexistence of free hydroxyl and hydrogen-bonding hydroxyl groups. The multiscale microstructure yielded starch-based hydrogels with favorable water-retention capabilities, high transparency (86.39 %), improved self-adhesive and self-healing properties, excellent stretchability (146 %), tissue-like ultra-softness (Young's modulus <10 kPa), and anti-freezing properties (<−50 °C). Overall, this study systematically elucidates the underlying mechanisms of CaCl<sub>2</sub> impacts on starch gelatinization, retrogradation, and hydrogel properties, paving the way for the on-demand functionality of starch hydrogels through regulated crystallization.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"357 ","pages":"Article 123371"},"PeriodicalIF":10.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon dots and cellulose nanocrystal-incorporated chitosan composite films with enhanced gas selectivity and photodynamic antibacterial properties for fruit preservation

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-17 DOI: 10.1016/j.carbpol.2025.123413

Bingzhen Liu , Kun Wang , Fangfei Sun , Yongliang Zhuang , Hui Li , Lincai Peng

{"title":"Carbon dots and cellulose nanocrystal-incorporated chitosan composite films with enhanced gas selectivity and photodynamic antibacterial properties for fruit preservation","authors":"Bingzhen Liu , Kun Wang , Fangfei Sun , Yongliang Zhuang , Hui Li , Lincai Peng","doi":"10.1016/j.carbpol.2025.123413","DOIUrl":"10.1016/j.carbpol.2025.123413","url":null,"abstract":"<div><div>Winter jujube is prone to rapid ripening and deterioration during postharvest storage and transportation. This study developed a multifunctional chitosan (CS) composite film incorporating dialdehyde cellulose nanocrystal (DCNC) and carbon dots (CDs) derived from walnut green husks. DCNC functioned as cross-linking filler, while CDs served as active nanofillers. The CS composite film containing 3 wt% DCNC and 3 wt% CDs (CS/DCNC/CDs-3 %) exhibited significant improvements in UV-blocking, mechanical strength, gas barrier, and thermal stability, attributing to the formation of a dense film structure and synergistic effect of Schiff base and hydrogen bonding interactions among film components. Notably, this composite film demonstrated a high CO<sub>2</sub>/O<sub>2</sub> selectivity of 14.1, facilitating the creation of an ideal atmosphere inside the package. This composite film exhibited remarkable antioxidant activity and displayed potent antibacterial capacity through photocatalytic ROS generation, achieving inhibition rates of 90.8 % against <em>S. aureus</em> and 86.8 % against <em>E. coli</em>. Additionally, this film showed favorable biosafety and biodegradability, addressing food safety and environmental concerns. Postharvest preservation tests confirmed that this film assisted by light exposure effectively regulated physiological metabolism and delayed the ripening of winter jujube, highlighting its potential as an active modified atmosphere packaging material for perishable fruit preservation.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"356 ","pages":"Article 123413"},"PeriodicalIF":10.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional hydrogel dressing composed of trichosanthes polysaccharide and carboxymethyl chitosan accelerates cachectic wound healing and reduces scar hyperplasia

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-17 DOI: 10.1016/j.carbpol.2025.123378

Guihua Chen , Chenxiao Yang , Xingyu Xu , Lu Yang , Yingyan Zhang , Chao Cai , Bahtiyor Muhitdinov , Abbaskhan Turaev , Hong Qiu , Shaogang Huang , Qin Xu , Huiyuan Wang , Yongzhuo Huang

{"title":"Multifunctional hydrogel dressing composed of trichosanthes polysaccharide and carboxymethyl chitosan accelerates cachectic wound healing and reduces scar hyperplasia","authors":"Guihua Chen , Chenxiao Yang , Xingyu Xu , Lu Yang , Yingyan Zhang , Chao Cai , Bahtiyor Muhitdinov , Abbaskhan Turaev , Hong Qiu , Shaogang Huang , Qin Xu , Huiyuan Wang , Yongzhuo Huang","doi":"10.1016/j.carbpol.2025.123378","DOIUrl":"10.1016/j.carbpol.2025.123378","url":null,"abstract":"<div><div>Cancer cachexia affects up to 80 % of advanced cancer patients and contributes to significant mortality. Impaired wound healing in cachectic patients limits physical activity, leads to nutrient loss, and increases infection risk. This study develops multifunctional hydrogels composed of oxidized polysaccharides (TPS) from Radix Trichosanthis and carboxymethyl chitosan (termed CMOT) to enhance wound healing and mitigate scar hyperplasia. TPS, characterized by its immunomodulatory properties, was oxidized to create aldehyde derivatives (oTPS1 and oTPS2) with varying oxidation levels and crosslinked with carboxymethyl chitosan through Schiff base reactions to form hydrogels (CMOT1 and CMOT2). It indicates the ability to tailor the rheological and mechanical properties of CMOT hydrogels through controlled oxidation and cross-linking. These hydrogels exhibited excellent self-healing properties, biocompatibility, and immunoregulatory effects on macrophages and T lymphocytes. Notably, CMOT2 hydrogel, with higher aldehyde content, exhibited superior mechanical properties, enhanced water retention, and slower degradation than CMOT1, consequently, accelerating wound healing in cancer cachexia conditions and reducing scar hyperplasia. The therapeutic mechanisms were associated with promoting angiogenesis, collagen synthesis, and epithelial repair, while down-regulating En-1. It not only addresses the challenges of wound healing in cancer cachexia but also offers a potential therapeutic strategy for scar hyperplasia inhibition.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"357 ","pages":"Article 123378"},"PeriodicalIF":10.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bespoke hydroxypropyl methylcellulose-based solid foams loaded with poorly soluble drugs by tunable modular design

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-16 DOI: 10.1016/j.carbpol.2025.123397

Wuzhong He, Huiling Mu, Natalja Genina

{"title":"Bespoke hydroxypropyl methylcellulose-based solid foams loaded with poorly soluble drugs by tunable modular design","authors":"Wuzhong He, Huiling Mu, Natalja Genina","doi":"10.1016/j.carbpol.2025.123397","DOIUrl":"10.1016/j.carbpol.2025.123397","url":null,"abstract":"<div><div>A tunable modular design (TMD) as a new approach was proposed to tailor the dose and drug release profile of poorly water-soluble drugs from hydroxypropyl methylcellulose (HPMC)-based solid foams by combining two manufacturing principles: (1) freeze-drying aqueous HPMC-based gels to yield porous sturdy modules with specific doses of an active pharmaceutical ingredient (API) with the step size of 3 mg, and (2) fine-tuning the desired dose of the API with the step size of 0.1 mg by inkjet printing of the API-loaded ink onto the modules. Carvedilol (CAR) was used as a model poorly water-soluble API that requires frequent dose adjustment. The limitation of poor CAR solubility was overcome by designing pharmaceutically approved co-solvent systems. This approach ensured printable inks of a high drug content, and sturdy and flexible modules with uniform distribution of CAR to achieve effective and accurate doses of CAR. The tailored release rate of CAR from TMD products was succeeded by varying the composition, particularly, the content and grade of HPMC, and physical dimensions of modules. The TMD approach holds potential for designing bespoke high-quality products, containing hydrophilic cellulose ethers such as HPMC and poorly water-soluble APIs.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"357 ","pages":"Article 123397"},"PeriodicalIF":10.7,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polygonatum cyrtonema Hua polysaccharide alleviates ulcerative colitis via gut microbiota-independent modulation of inflammatory immune response

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-02-16 DOI: 10.1016/j.carbpol.2025.123387

Jin-Yang Shi , Yong-Jian Wang , Qian-Wen Bao , Ya-Min Qin , Pei-Pei Li , Qiao-Qiao Wu , Cheng-Kai Xia , De-Ling Wu , Song-Zi Xie

{"title":"Polygonatum cyrtonema Hua polysaccharide alleviates ulcerative colitis via gut microbiota-independent modulation of inflammatory immune response","authors":"Jin-Yang Shi , Yong-Jian Wang , Qian-Wen Bao , Ya-Min Qin , Pei-Pei Li , Qiao-Qiao Wu , Cheng-Kai Xia , De-Ling Wu , Song-Zi Xie","doi":"10.1016/j.carbpol.2025.123387","DOIUrl":"10.1016/j.carbpol.2025.123387","url":null,"abstract":"<div><div><em>Polygonatum cyrtonema</em> polysaccharides (PCP) exhibit ameliorative effects on colitis. However, whether the protective effects of PCP depend on the gut microbiota and how PCP regulates intestinal immune responses to alleviate colitis remain unclear. Therefore, this study investigated the effect of PCP against colitis focusing on the regulation of intestinal immune response. The PCP structure was reclassified as fructan. PCP treatment significantly reduced the symptoms of colitis. PCP restored IgA, ZO-1, Occludin, and MUC2 expression to enhance intestinal barrier function. Oral PCP administration markedly inhibited excessive inflammation-mediated immune response by modulating inflammatory cytokines secretion and Th17/Tregs cell balance and restored gut microbial composition. Interestingly, PCP still had a significant ameliorating effect on intestinal inflammation in colitis mice with gut microbial depletion by antibiotics. In the Caco-2/RAW264.7 co-culture inflammation model, PCP treatment improved the intestinal epithelial barrier function by regulating the inflammatory immune response through signal transduction pathways. Overall, these findings suggested that the alleviating effects of PCP on colitis are independent of gut microbiota, and that PCP can directly modulate the inflammatory immune response and intestinal barrier function, which in turn regulates gut microbiota. These findings will provide new insights into the action mechanism of natural polysaccharides in relieving colitis.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"356 ","pages":"Article 123387"},"PeriodicalIF":10.7,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0