Carbohydrate Polymers最新文献_第10页

Dual role of ionic liquid modified β-Cyclodextrin in dynamic conformational regulation and substrate enrichment for improved lipase immobilization 离子液体修饰β-环糊精在动态构象调节和底物富集中的双重作用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-21 DOI: 10.1016/j.carbpol.2025.123786

Liran Ji , Jiaxiang Du , Wei Zhang , Zeping Lu , Binbin Nian , Yi Hu

{"title":"Dual role of ionic liquid modified β-Cyclodextrin in dynamic conformational regulation and substrate enrichment for improved lipase immobilization","authors":"Liran Ji , Jiaxiang Du , Wei Zhang , Zeping Lu , Binbin Nian , Yi Hu","doi":"10.1016/j.carbpol.2025.123786","DOIUrl":"10.1016/j.carbpol.2025.123786","url":null,"abstract":"<div><div>To address the limitations of free enzymes in industrial applications, immobilization technologies have garnered significant attention as a robust strategy for enhancing operational stability and reusability. In this work, a novel-designed immobilized lipase (Fe<sub>3</sub>O<sub>4</sub>@β-CD-IL-CRL) was successfully developed via the introduction of β-cyclodextrin (β-CD), Fe<sub>3</sub>O<sub>4</sub> nanoparticles and ionic liquid (IL). The properties studies indicated that Fe<sub>3</sub>O<sub>4</sub>@β-CD-IL-CRL exhibited remarkable stability, maintaining 72.8 % residual activity after 7 successive catalytic cycles and retaining more than 80 % of its initial activity following 2 h incubation at 60 °C. Furthermore, to examine the practical applications of Fe<sub>3</sub>O<sub>4</sub>@β-CD-IL-CRL, it was used in the synthesis of phytosterol esters. The results suggested that a yield of 95.0 % can be obtained at 50 °C in 48 h, highlighting its exceptional catalytic efficiency. Molecular dynamics (MD) simulations showed that the immobilization of Fe<sub>3</sub>O<sub>4</sub>@β-CD-IL maintained an optimal pocket size for CRL, which was advantageous for substrate anchoring. Additionally, analyses of the lid structure distance and tunnel further supported this finding. Beyond the impact on the dynamic conformation of lipase, β-CD was also found to effectively enrich substrates, enhancing catalytic efficiency. This discovery offers new insights into the optimization of biocatalytic systems and highlights the potential of β-CD as a versatile carrier for enzyme immobilization.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"364 ","pages":"Article 123786"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulose nanocrystals-supported organocatalyst for the synthesis of cyclic carbonates via cycloaddition of carbon dioxide to epoxide 纤维素纳米晶负载的有机催化剂，用于二氧化碳到环氧化物的环加成合成环状碳酸盐

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-21 DOI: 10.1016/j.carbpol.2025.123762

Nouaamane El Idrissi , Hicham Aitbella , Nicolas Merle , Frédéric Cazaux , João P. Prates Ramalho , Larbi Belachemi , Hamid Kaddami , Philippe Zinck

{"title":"Cellulose nanocrystals-supported organocatalyst for the synthesis of cyclic carbonates via cycloaddition of carbon dioxide to epoxide","authors":"Nouaamane El Idrissi , Hicham Aitbella , Nicolas Merle , Frédéric Cazaux , João P. Prates Ramalho , Larbi Belachemi , Hamid Kaddami , Philippe Zinck","doi":"10.1016/j.carbpol.2025.123762","DOIUrl":"10.1016/j.carbpol.2025.123762","url":null,"abstract":"<div><div>The synthesis of cyclic carbonates can be realized via cycloaddition of CO<sub>2</sub> to epoxides. Organocatalysts are of interest regarding specific applications. The reaction can be co-activated by a hydrogen bond donor such as cellulose. A fully recyclable catalytic system combining an organocatalyst and a polysaccharide hydrogen bond donor has never been reported. In this study, we have supported an organocatalyst onto cellulose nanocrystals (CNC). The grafting of 1,5,7-triazabicyclo [4.4.0] dec-5-ene (TBD) onto microcrystalline cellulose (MCC) and onto the surface of cellulose nanocrystals (CNC) was confirmed by <sup>13</sup>C solid state NMR, FT-IR, XRD and elemental analysis. The prepared heterogeneous TBD-CNC nanocatalyst was successfully assessed on a scope of five epoxides including mono- and di-substituted. The activity was found to be higher than that of the TBD-MCC supported catalyst, and higher than the homogeneous counterpart. That latter result highlights the coactivation role of the polysaccharide. Recyclability studies showed that the catalyst is reusable for at least five cycles. Density functional theory (DFT) studies indicate that the mechanism most probably involves the formation of anionic cellulosic species. Initially, the grafted TBD deprotonates a surface hydroxy to form a cellulose alkoxide, which then performs a nucleophilic attack on CO<sub>2</sub> or on the activated epoxide.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"364 ","pages":"Article 123762"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mussel inspired carboxymethyl cellulose/pectin composite hydrogel with photothermal enhanced antibacterial property for burn wound healing 贻贝启发羧甲基纤维素/果胶复合水凝胶光热增强抗菌性能烧伤伤口愈合

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-21 DOI: 10.1016/j.carbpol.2025.123780

Liping Yin , Zhe Cui , Jintao Ma , Weichen Sun , Yu Zhang , Yong Wang , Wenjuan Li , Xianling Wang , Jianglei Qin

{"title":"Mussel inspired carboxymethyl cellulose/pectin composite hydrogel with photothermal enhanced antibacterial property for burn wound healing","authors":"Liping Yin , Zhe Cui , Jintao Ma , Weichen Sun , Yu Zhang , Yong Wang , Wenjuan Li , Xianling Wang , Jianglei Qin","doi":"10.1016/j.carbpol.2025.123780","DOIUrl":"10.1016/j.carbpol.2025.123780","url":null,"abstract":"<div><div>The burn wounds are particularly susceptible to bacterial infection, while the hydrogel wound dressings with antibacterial property are expected to promote burn wound repairing progress. In this research, carboxymethyl cellulose bearing hydrazide groups and hemostatic polyphosphate moiety (CHP) was synthesized and cross-linked with dopamine grafted oxidized pectin (OPD) to prepare hemostatic self-healing hydrogel for burn wound repairing. Polydopamine coated graphene oxide (PGO) was loaded into the hydrogel to endow it with photothermal stimulated antibacterial property and enhanced the ROS scavenging ability, which can inhibit bacterial infection and promote tissue regeneration. Moreover, the loaded tannic acid (TA) further enhanced the antioxidant and anti-inflammatory properties of the hydrogel. As a result, the photothermal property of PGO and TA greatly reduced bacterial infection and promoted cell proliferation both <em>in vitro</em> and <em>in vivo</em>. More importantly, the composite PGO/TA/Gel hydrogel exhibited satisfactory therapeutic effect in burn wound healing in mice model through reducing inflammation response, enhance the collagen deposition and promote angiogenesis based on histological analysis. These advantages make this composite hydrogel great potential applications in the field of wound dressings in biomedication.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"364 ","pages":"Article 123780"},"PeriodicalIF":10.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silylation of wheat straw cellulose pulp for its valorization as rheology modifier of industrial hydrophobic fluids: Cases of castor oil and bitumen 麦草纤维素浆作为工业疏水流体流变改性剂的硅基化：以蓖麻油和沥青为例

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-20 DOI: 10.1016/j.carbpol.2025.123778

M. Trejo-Cáceres, J.E. Martín-Alfonso, J.M. Franco

{"title":"Silylation of wheat straw cellulose pulp for its valorization as rheology modifier of industrial hydrophobic fluids: Cases of castor oil and bitumen","authors":"M. Trejo-Cáceres, J.E. Martín-Alfonso, J.M. Franco","doi":"10.1016/j.carbpol.2025.123778","DOIUrl":"10.1016/j.carbpol.2025.123778","url":null,"abstract":"<div><div>This work explores an innovative approach to developing novel oleo- and bitumen-based dispersions, which are potentially applicable as semisolid lubricants and bitumen binders, respectively, using lignocellulosic biomass. In particular, it focuses on investigating the impact of the silylation process of wheat straw Kraft cellulose pulp (KWP) on the rheological properties of these dispersions. To this end, KWP was functionalized with <em>tert</em>-butyldimethylsilyl chloride (TBDMSCl), using imidazole (ImH) as a catalyst, through a simple and efficient method. Different conditions of the silylation process such as temperature (20–100 °C), TBDMSCl/ImH ratio (1/2.5–2.5/2.5), and silylation time (2–24 h) were applied to illustrate the flexibility of the proposed methodology. Modified pulps with oil and/or bitumen structuring ability were obtained, as a result of the interaction between the hydrophobic alkyl-siloxane segment-containing fibers and these hydrophobic fluids, which enhance the compatibility between both components. Oleo-dispersions with a similar viscoelastic response to lithium grease, used as a benchmark, exhibited significantly reduced coefficients of friction and wear. Meanwhile, bitumen-dispersions displayed a substantial improvement in stiffness and elasticity with the temperature. This work may offer a novel strategy for developing rheology modifiers or structuring agents for industrial hydrophobic fluids.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"364 ","pages":"Article 123778"},"PeriodicalIF":10.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of salicylic acid-derivatized alginate: Synthesis, characterization, and anti-acne potential 水杨酸衍生藻酸盐的综合分析：合成、表征和抗痤疮潜力

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-20 DOI: 10.1016/j.carbpol.2025.123776

Mengyao Yuan , Lijia Liu , Xiaoqing Ma , Lihua Geng , Xiuliang Wang , Ning Wu , Yang Yue , Quanbin Zhang , Jing Wang

{"title":"Comprehensive analysis of salicylic acid-derivatized alginate: Synthesis, characterization, and anti-acne potential","authors":"Mengyao Yuan , Lijia Liu , Xiaoqing Ma , Lihua Geng , Xiuliang Wang , Ning Wu , Yang Yue , Quanbin Zhang , Jing Wang","doi":"10.1016/j.carbpol.2025.123776","DOIUrl":"10.1016/j.carbpol.2025.123776","url":null,"abstract":"<div><div>Salicylic acid (SA) is commonly utilized in the early treatment of acne due to its therapeutic efficacy. However, its clinical performance is hindered by limited aqueous solubility, stability and potential for irritation. This study addresses these challenges through deriving SA with alginate (SA-g-Alg) based on a complementary advantages strategy, designed to retain the bioactivity of SA while mitigating its drawbacks. A comprehensive investigation into the structure, physiochemical properties and anti-acne bioactivities of SA-g-Alg was conducted. The results demonstrate that SA-g-Alg achieved a 30-fold higher aqueous stability compared to free SA, along with enhanced moisturizing capacity and reduced cytotoxicity and skin irritation. These enhancements were attributed to the multi-hydroxy and inherent high biocompatibility of alginate. It is noteworthy that SA-g-Alg, with a grafting degree of 24.9 %, exhibited a synergistic effect, as evidenced by its superior lipid regulating ability, comparable antibacterial efficacy and prolonged anti-inflammatory bioavtivity compared to equivalent salicylic acid group in free SA. Moreover, <em>in vivo</em> studies employing a rabbit ear acne model show that a 2 % SA-g-Alg formulation was as effective as a clinical established 2 % SA ointment in therapeutic management of acne. In conclusion, this study underscores the enhanced physiochemical and anti-acne properties of SA-g-Alg, highlighting its potential as a promising therapeutic agent for the treatment of mild to moderate acne.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"364 ","pages":"Article 123776"},"PeriodicalIF":10.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delivery of penetration-enhancing antioxidant polyphenol nanoparticles with Codonopsis pilosula polysaccharide microneedles for synergistic treatment of psoriasis 抗氧化多酚纳米颗粒与党参多糖微针协同治疗银屑病

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-19 DOI: 10.1016/j.carbpol.2025.123777

Siqi Liu , Silu Song , Yu Zhang , Liming Yan , Yuhui Chen , Wanrong Li , Banaz Jalil , Chun Wu , Yuping Fu , Xingfu Chen , Jingyi Gu , Lixia Li , Zhongqiong Yin , Xu Song , Mengliang Tian , Yuanfeng Zou

{"title":"Delivery of penetration-enhancing antioxidant polyphenol nanoparticles with Codonopsis pilosula polysaccharide microneedles for synergistic treatment of psoriasis","authors":"Siqi Liu , Silu Song , Yu Zhang , Liming Yan , Yuhui Chen , Wanrong Li , Banaz Jalil , Chun Wu , Yuping Fu , Xingfu Chen , Jingyi Gu , Lixia Li , Zhongqiong Yin , Xu Song , Mengliang Tian , Yuanfeng Zou","doi":"10.1016/j.carbpol.2025.123777","DOIUrl":"10.1016/j.carbpol.2025.123777","url":null,"abstract":"<div><div>Poor drug penetration due to local skin thickening and microenvironmental redox imbalance remain a critical challenge in the treatment of epidermal diseases. Considering that oxidative stress due to the overproduction of Reactive Oxygen Species (ROS) in the skin microenvironment is one of the main mechanisms in the pathogenesis of psoriasis, we developed a ROS-sensitive transdermal drug delivery system to enhance topical treatment of psoriasis. Cross-linked cyclodextrin metal-organic framework (COF) containing curcumin (CUR) was dispersed in two types of dissolving polysaccharide microneedles (CPPAP-MNs-CUR@COF, CPPNP-MNs-CUR@COF) made from <em>Codonopsis pilosula</em> neutral polysaccharides (CPPNP) and acidic polysaccharides (CPPAP) for topical administration. COF contains peroxyoxalate bonds that scavenge H<sub>2</sub>O<sub>2</sub>, hydrolyse and eliminate ROS generated at the site of inflammation. Microneedles (MNs) that break through the skin's physical barrier improve drug penetration, while <em>Codonopsis pilosula</em> Polysaccharides (CPP) can synergise with CUR to significantly attenuate inflammation and oxidative stress and ameliorate imiquimod (IMQ)-induced symptoms of psoriasis in mice through inhibition of the IL-23/IL-17 inflammatory axis. It is a promising drug delivery system that is expected to provide new strategies for the topical treatment of psoriasis in the clinical setting.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"363 ","pages":"Article 123777"},"PeriodicalIF":10.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tree thorns-inspired hyaluronic acid-based dissolving microneedles loaded with salvianolic acid B for effective hypertrophic scar treatment with enhanced long-term stability 树刺启发透明质酸为基础的溶解微针装载丹酚酸B有效的增生性疤痕治疗与增强的长期稳定性

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-17 DOI: 10.1016/j.carbpol.2025.123773

Bricard Mbituyimana , Longjun Luo , Fuyu Qi , Yuchi Jiang , Omar Mohammad Atta , Mohamed S. Kishta , Mostafa Maboruk , Min Wu , Wei Ge , Zhijun Shi , Guang Yang

{"title":"Tree thorns-inspired hyaluronic acid-based dissolving microneedles loaded with salvianolic acid B for effective hypertrophic scar treatment with enhanced long-term stability","authors":"Bricard Mbituyimana , Longjun Luo , Fuyu Qi , Yuchi Jiang , Omar Mohammad Atta , Mohamed S. Kishta , Mostafa Maboruk , Min Wu , Wei Ge , Zhijun Shi , Guang Yang","doi":"10.1016/j.carbpol.2025.123773","DOIUrl":"10.1016/j.carbpol.2025.123773","url":null,"abstract":"<div><div>Hypertrophic scar (HS) represents a natural consequence of abnormal fibroproliferation occurring during wound healing, which not only leads to tissue dysfunction but also causes psychological issues for patients. Up to now, no completely effective therapy for HS has been found. Salvianolic acid B (Sal<img>B), one of the natural plant-derived compounds, has the capacity to suppress the fibrosis process in scarring treatment. Nevertheless, due to its poor skin-penetration ability and unsatisfactory stability in existing formulations, its current clinical applicability is restricted. In this study, inspired by the separation behavior of the thorns on the trunks of the silk floss tree, we developed a hyaluronic acid (HA) dissolving microneedle (MN) platform loaded with Sal<img>B. This platform can enhance the sustainable delivery of Sal-B directly into scar tissue in a minimally invasive manner, thereby enhancing the efficacy of HS therapy and improving the stability of Sal<img>B. To evaluate the therapeutic efficacy of Sal-B MN, we established a rabbit ear HS model. After being administered five times, Sal-B MN significantly reduced the scar elevation index (SEI) and down-regulated the expressions of collagen I, transforming growth factor beta 1 (TGF-β1), Signal Transducer and Activator of Transcription 3 (STAT3), and alpha-smooth muscle actin (α-SMA). Notably, these effects were still evident even after six months of storage at room temperature. This indicates that tree-inspired Sal-B MN holds great potential as a highly effective tool for HS treatment with a long shelf-life. This makes the system suitable for clinical application and storage under various conditions.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"364 ","pages":"Article 123773"},"PeriodicalIF":10.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Thermo/photo dual-crosslinking chitosan-gelatin methacrylate hydrogel T with controlled shrinking property for contraction fabrication” [Carbohydrate Polymers 236 (2020) 116067] “热/光双交联壳聚糖-明胶甲基丙烯酸酯水凝胶T与控制收缩性能的收缩制造”的勘误表[碳水化合物聚合物236 (2020)116067]

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-17 DOI: 10.1016/j.carbpol.2025.123700

Xin Luo , Yidan Liu , Jianhui Pang , Shichao Bi , Zhongzheng Zhou , Zhe Lu , Chao Feng , Xiguang Chen , Ming Kong

引用次数: 0

Human gut Bifidobacterium longum subsp. suillum is enriched in vitro by a pectic polysaccharide isolated from the flowers of Lilium lancifolium 人肠道长双歧杆菌亚种。从百合花中分离的果胶多糖在体外富集了suillum

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-17 DOI: 10.1016/j.carbpol.2025.123772

Meixia Li , Renjie Liu , Tao Wang , Lana Duo , Guo Chen , Mengfei Mao , Yue Sun , Yun Li , Simin Cai , Wanqi Zhou , Haibiao Lai , Kan Ding

{"title":"Human gut Bifidobacterium longum subsp. suillum is enriched in vitro by a pectic polysaccharide isolated from the flowers of Lilium lancifolium","authors":"Meixia Li , Renjie Liu , Tao Wang , Lana Duo , Guo Chen , Mengfei Mao , Yue Sun , Yun Li , Simin Cai , Wanqi Zhou , Haibiao Lai , Kan Ding","doi":"10.1016/j.carbpol.2025.123772","DOIUrl":"10.1016/j.carbpol.2025.123772","url":null,"abstract":"<div><div>Although pectins have been explored widely, knowledge of their effects on the gut microbiota is lacking owing their complex structure. The aim of this study was to investigate whether pectin enriched gut microbes <em>in vitro</em>. To address this, a homogeneous RG-I like pectin, L01-B1, with a molecular weight of 43.9 kDa was extracted from the flowers of <em>Lilium lancifolium</em>. Structural analysis revealed that L01-B1 contained rhamnose, glucuronic acid, galacturonic acid, galactose, and arabinose in a molar ratio of 13.4: 1.8: 11.1: 37.2: 36.5. The backbone of L01-B1 was composed of 1, 6-β-Gal<em>p</em>, 1, 4-α-Gal<em>p</em>A, and 1, 2-α-Rha<em>p</em>, whereas the branches included 1, 5-α-Ara<em>f</em>, 1, 4-β-Gal<em>p</em>, and T-β-Glc<em>p</em>A attached to C-4 of rhamnose, and 1, 3-β-Gal<em>p</em> and T-β-Gal<em>p</em> linked to C-3 of galactose. L01-B1 altered the composition of human gut microbiota <em>in vitro</em> and increased the abundance of <em>Bifidobacterium longum</em>. Furthermore, <em>Bifidobacterium longum</em> subsp. <em>suillum</em> strain DK001 was isolated and identified from human feces. The DK001 genome was found to be circular with a genome size of approximately 2.4 M. Notably, L01-B1 might possibly be degraded by two kinds of enzymes and change the metabolism of human gut microbiota. Overall, these findings provide insights into intervention strategies that target <em>Bifidobacterium longum</em>.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"364 ","pages":"Article 123772"},"PeriodicalIF":10.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the masking of berberine bitterness by natural polysaccharides based on a “net-hook” dual-effect mechanism 基于“网钩”双效机制探索天然多糖对小檗碱苦味的掩蔽作用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-05-17 DOI: 10.1016/j.carbpol.2025.123771

Min Qiu , Jiaxin Li , Huijuan Xie , Jiamin Feng , Qi Huang , Yin Tian , Haozhou Huang , Li Han , Dingkun Zhang

{"title":"Exploring the masking of berberine bitterness by natural polysaccharides based on a “net-hook” dual-effect mechanism","authors":"Min Qiu , Jiaxin Li , Huijuan Xie , Jiamin Feng , Qi Huang , Yin Tian , Haozhou Huang , Li Han , Dingkun Zhang","doi":"10.1016/j.carbpol.2025.123771","DOIUrl":"10.1016/j.carbpol.2025.123771","url":null,"abstract":"<div><div>Bitterness masking is a crucial technical hurdle in the development and application of oral liquid medicines. Small-molecule excipients, such as cyclodextrin, often provide insufficient bitterness masking, thus prompting the exploration of large-molecule excipients, especially polysaccharides. In this study, the bitterness-masking effects of 10 polysaccharides were assessed using berberine as a model. Only iota-carrageenan significantly reduced the bitterness of berberine, decreasing it from strong to slight. A comparative study of the bitterness-masking mechanisms of three polysaccharides revealed that carrageenan polymer chains formed an interwoven network, decreasing the release of berberine by 40 %. Moreover, -OSO₃<sup>−</sup> groups on the polymer chains exhibited strong electrostatic interactions with the N<sup>+</sup> in berberine, significantly reducing the electron cloud density of N<sup>+</sup>. This combined effect traps berberine within the “net-hook” structure of the iota-carrageenan, markedly weakening the activation of bitter receptors by the berberine. Ultrasonic treatment-induced disruption of the iota-carrageenan network structure yielded a 29 % reduction in bitterness-masking efficacy, while carrageenan with a lower -OSO₃<sup>−</sup> content achieved a 40 % reduction in efficacy. In conclusion, the “net-hook” structure of natural polysaccharides is a necessary condition for them to exert a bitterness-masking effect. This finding provides a scientific basis for expanding the applications of natural polysaccharides in taste-masking.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"364 ","pages":"Article 123771"},"PeriodicalIF":10.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0