Carbohydrate Polymers最新文献_第10页

Harnessing the polysaccharide production potential to optimize and expand the application of probiotics 利用多糖生产潜力优化和扩大益生菌的应用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-07 DOI: 10.1016/j.carbpol.2024.122951

Dong-Xu Ma , Hui-Juan Cheng , Hui Zhang , Shuo Wang , Xiao-Tao Shi , Xin Wang , Da-Chun Gong

{"title":"Harnessing the polysaccharide production potential to optimize and expand the application of probiotics","authors":"Dong-Xu Ma , Hui-Juan Cheng , Hui Zhang , Shuo Wang , Xiao-Tao Shi , Xin Wang , Da-Chun Gong","doi":"10.1016/j.carbpol.2024.122951","DOIUrl":"10.1016/j.carbpol.2024.122951","url":null,"abstract":"<div><div>Certain probiotic microorganisms can synthesize important bioproducts, including polysaccharides as components of cellular structure or extracellular matrix. Probiotic-derived polysaccharides have been widely applied in food, pharmaceutical, and medical fields due to their excellent properties and biological activities. The development of polysaccharide production potential has become a driving force for facilitating biotechnological applications of probiotics. Based on technical advances in synthetic biology, significant progress has recently been made in engineering probiotics with efficient biosynthesis of polysaccharides. Herein, this review summarizes probiotics chassis and genetic tools used for polysaccharide production. Then, probiotic polysaccharides and relevant biosynthesis mechanisms are also clearly described. Next, we introduce strategies for preparing high-yield, controllable molecular weight or non-native polysaccharides by adjusting metabolic pathways and integrating expression elements in probiotics. Finally, some prospective and well-established contributions of exogenous and <em>in situ</em> polysaccharides in probiotics' stability, bioactivity, and therapeutic effects are presented. Our viewpoints on advancing the efficient biomanufacturing of valuable biopolymers in probiotics and engineering probiotics with customized features are provided to exploit probiotics' industrial and biomedical applications.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122951"},"PeriodicalIF":10.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyaluronic acid-functionalized ruthenium photothermal nanoenzyme for enhancing osteosarcoma chemotherapy: Cascade targeting and bidirectional modulation of drug resistance 用于增强骨肉瘤化疗的透明质酸功能化钌光热纳米酶：级联靶向和双向调节耐药性

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-06 DOI: 10.1016/j.carbpol.2024.122945

Weifeng Chen , Hao Feng , Yinyin Mo, Zhihui Pan, Shichen Ji, Hong Liang, Xing-Can Shen, Bang-Ping Jiang

{"title":"Hyaluronic acid-functionalized ruthenium photothermal nanoenzyme for enhancing osteosarcoma chemotherapy: Cascade targeting and bidirectional modulation of drug resistance","authors":"Weifeng Chen , Hao Feng , Yinyin Mo, Zhihui Pan, Shichen Ji, Hong Liang, Xing-Can Shen, Bang-Ping Jiang","doi":"10.1016/j.carbpol.2024.122945","DOIUrl":"10.1016/j.carbpol.2024.122945","url":null,"abstract":"<div><div>Insufficient drug delivery efficiency <em>in vivo</em> and robust drug resistance are two major factors to induce suboptimal efficacy in chemotherapy of osteosarcoma (OS). To address these challenges, we developed polysaccharide hyaluronic acid (HA)-functionalized ruthenium nanoaggregates (Ru NAs) to enhance the chemotherapy of doxorubicin (DOX) for OS. These NAs, comprising Ru nanoparticles (NPs) and alendronate-modified HA (HA-ALN), effectively load DOX, resulting in DOX@Ru-HA-ALN NAs. The combination of HA and ALN in NAs ensures outstanding cascade targeting towards tumor-invaded bone tissues and CD44-overexpressing tumor cells, maximizing therapeutic efficacy while minimizing off-target effects. Concurrently, the Ru NPs in NAs function as “smart” photoenzymatic agent to not only <em>in situ</em> relieve hypoxia of OS <em>via</em> the catalysis of overexpressed H<sub>2</sub>O<sub>2</sub> to produce O<sub>2</sub>, but also generate mild photothermal effect under 808-nm laser irradiation. They can bidirectionally overcome drug resistance of DOX <em>via</em> downregulation of resistance-related factors including multi-drug resistant associate protein, P-glycoprotein, heat shock factor 1, <em>etc.</em> The integration of cascade targeting with bidirectional modulation of drug resistance positions Ru-HA-ALN NAs to substantially enhance DOX chemotherapy for OS. Therefore, the present work highlights the potential of polysaccharide-functionalized nanomaterials in advancing tumor chemotherapy by addressing challenges of both delivery efficiency and drug resistance.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122945"},"PeriodicalIF":10.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of CNF ratio and pressure on structural and electrochemical performance of hybrid hydrogel for flexible free-standing electrode and sensors CNF 比率和压力对用于柔性独立电极和传感器的混合水凝胶的结构和电化学性能的影响

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-06 DOI: 10.1016/j.carbpol.2024.122955

Xiping Ding, Jiajia Shan, Shuting Yang, Junyan Liu, Chen Jiang, Shitao Yu, Qiong Wu

{"title":"Effect of CNF ratio and pressure on structural and electrochemical performance of hybrid hydrogel for flexible free-standing electrode and sensors","authors":"Xiping Ding, Jiajia Shan, Shuting Yang, Junyan Liu, Chen Jiang, Shitao Yu, Qiong Wu","doi":"10.1016/j.carbpol.2024.122955","DOIUrl":"10.1016/j.carbpol.2024.122955","url":null,"abstract":"<div><div>A 2D flexible hydrogel (GO/CNF<sub>n</sub>) with layered structure and superhydrophilic is synthesized via cross-linking and self-assembling of graphene oxide (GO) with cellulose nanofiber (CNF) through microwave-assisted hydrothermal. CNF acts as “dispersant” and “spacer”, making GO nanosheets uniformly disperse on their surface with less agglomerations. The carboxyl groups and hydrophilicity of CNF effectively improve the charge storage capacity of carbon materials through interactions. When the mass ratio of GO to CNF is 3:1, the GO/CNF<sub>1</sub> exhibits an excellent comprehensive electrochemical performance as free-standing electrodes, with the specific capacitance reaching 295 F/g at 0.5 A/g in three-electrode system. The influence of press pressure on GO/CNF<sub>n</sub> reveals that increasing the pressure improves the hydrophilicity of the electrode, favoring their wettability to aqueous electrolyte. GO/CNF<sub>1</sub>–6 possesses the highest degree of graphitization, and delivers a highest mass specific capacitance up to 493 F/g at 0.5 A/g. Flexible solid-state symmetric supercapacitor with GO/CNF<sub>1</sub>–6 as electrodes exhibits an energy density of 20.6 Wh/kg at a power density of 250 W/kg. The good flexibility and biocompatibility of the devices show sensitive current response to biological signals, endowing them potential application prospect in wearable portable electronics and human motion detections.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122955"},"PeriodicalIF":10.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mussel-inspired modified regenerated cellulose as tissue adhesive and antibacterial gauze: A promising approach for rapid hemostasis in non-compressible hemorrhage 贻贝启发的改性再生纤维素作为组织粘合剂和抗菌纱布：在非可压缩性出血中快速止血的有效方法

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-06 DOI: 10.1016/j.carbpol.2024.122949

Nastaran Alipour , Salar Salmanipour , Ali Rezaie , Hassan Amini , Maryam Ghahremani-Nasab , Ahmad Mehdipour , Roya Salehi

{"title":"Mussel-inspired modified regenerated cellulose as tissue adhesive and antibacterial gauze: A promising approach for rapid hemostasis in non-compressible hemorrhage","authors":"Nastaran Alipour , Salar Salmanipour , Ali Rezaie , Hassan Amini , Maryam Ghahremani-Nasab , Ahmad Mehdipour , Roya Salehi","doi":"10.1016/j.carbpol.2024.122949","DOIUrl":"10.1016/j.carbpol.2024.122949","url":null,"abstract":"<div><div>Uncontrollable hemorrhage leads to high mortality rates; thus, engineering effective hemostatic materials is crucial for rapid hemostasis. Developing hemostatic materials for rapid coagulation, antibacterial activity, and easy removal without compromising clot integrity remains a challenge. Herein, a multifunctional hemostatic gauze was engineered by modifying regenerated cellulose textile through multiple sequential chemical reactions, including carboxymethylation, crosslinking with CaCl<sub>2</sub>/ZnCl<sub>2</sub> solution, oxidation, and polymerization with dopamine. Provided gauze demonstrated remarkable wet-tissue adhesion (890 kPa) that physically sealing the area to prevent blood loss. The engineered gauze exhibits excellent antibacterial activity (against <em>S. aureus</em> and <em>E. coli</em>) and enhanced hemostatic ability (clotting time (20S), attachment of red blood cells (∼93 %) and platelets (∼80 %)). The complete hemostasis and stable clot formation without secondary bleeding were achieved by synthesized gauze in 20s, 50s, and 100s for the rat and rabbit liver, and rat femoral artery injury models, respectively. That was significantly faster hemostasis (4–14-fold reduction in time) and lower blood loss (3-fold reduction) compared to the commercial hemostatic textiles (<em>p</em> < 0.001). Biochemical, hematological, and pathological examinations revealed no evidence of systemic and local inflammation or toxic effects in the rat organs. The engineered hemostatic gauze exhibits outstanding characteristics of a hemostatic material for clinical applications.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122949"},"PeriodicalIF":10.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electro-responsive hyaluronic acid-based click-hydrogels for wound healing 用于伤口愈合的电响应透明质酸基点击水凝胶

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-06 DOI: 10.1016/j.carbpol.2024.122941

Víctor Castrejón-Comas , Nil Mataró , Leonor Resina , David Zanuy , Quim Nuñez-Aulina , Joel Sánchez-Morán , Hamidreza Enshaei , Marc Arnau , Helena Muñoz-Galán , Joshua C. Worch , Andrew P. Dove , Carlos Alemán , Maria M. Pérez-Madrigal

{"title":"Electro-responsive hyaluronic acid-based click-hydrogels for wound healing","authors":"Víctor Castrejón-Comas , Nil Mataró , Leonor Resina , David Zanuy , Quim Nuñez-Aulina , Joel Sánchez-Morán , Hamidreza Enshaei , Marc Arnau , Helena Muñoz-Galán , Joshua C. Worch , Andrew P. Dove , Carlos Alemán , Maria M. Pérez-Madrigal","doi":"10.1016/j.carbpol.2024.122941","DOIUrl":"10.1016/j.carbpol.2024.122941","url":null,"abstract":"<div><div>With the aim of healing challenging skin wounds, electro-responsive click-hydrogels made of hyaluronic acid (clickHA) crosslinked with a modified polyethylene glycol precursor (PEG) were prepared by semi-interpenetrating a conducting polymer, poly(hydroxymethyl-3,4-ethylenedioxythiophene) (PEDOT-MeOH) by oxidative polymerization. The porosity and pore size of the mixed hydrogel, clickHA/PEDOT-MeOH, were both higher than those determined for the hydrogel without PEDOT-MeOH, while a honeycomb-like morphology with PEDOT-MeOH covering the pore walls was observed. Although such PEDOT-MeOH-induced changes did not influence the water absorption capacity of clickHA, they drastically affected the mechanical and electrochemical behavior. More specifically, the semi-interpenetration of PEDOT-MeOH into clickHA resulted in an increase of the Young's modulus, the compressive strength and, especially, the electrochemical activity. The biocompatibility and the potential for skin regeneration of clickHA/PEDOT-MeOH were preliminary assessed using viability and wound-healing assays with epithelial cells. Not only is the conducting hydrogel formulation biocompatible, but also promotes efficient cell migration by electrostimulation using a small voltage (0.5 V) for a short time (15 min). Thus, in just 1 h the wound gap was repaired, and a homogeneous monolayer of migrated cells was formed.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122941"},"PeriodicalIF":10.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stabilizing effect of Leuconostoc mesenteroides Lm10 produced dextran in situ on stirred soy yogurt: Structure-function relationship 中肠联白色念珠菌 Lm10 在原位生产的葡聚糖对搅拌型大豆酸奶的稳定作用：结构-功能关系

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-05 DOI: 10.1016/j.carbpol.2024.122948

Yiqiang Dai , Zhe Wang , Zhongjiang Wang , Mingsheng Dong , Daoying Wang , Xiudong Xia

{"title":"Stabilizing effect of Leuconostoc mesenteroides Lm10 produced dextran in situ on stirred soy yogurt: Structure-function relationship","authors":"Yiqiang Dai , Zhe Wang , Zhongjiang Wang , Mingsheng Dong , Daoying Wang , Xiudong Xia","doi":"10.1016/j.carbpol.2024.122948","DOIUrl":"10.1016/j.carbpol.2024.122948","url":null,"abstract":"<div><div>Stirred soy yogurt as a dairy alternative is widely accepted among consumers, but its poor stability has been an urgent problem. We found that <em>Leuconostoc mesenteroides</em> Lm10 produced dextran reduced water mobility and improved the water holding capacity of stirred soy yogurt, especially with over 4 % sucrose added which could completely prevent whey separation. With the increase of dextran content, the particle size of stirred soy yogurt was significantly decreased, accompanied by the improvement of viscoelastic behaviors and resistance to deformation. Moreover, dextran had a stronger ability to maintain the stability of stirred soy yogurt in comparison with gelatin, xanthan and carrageenan during cold storage. The structure-function attributes of this dextran were also revealed. Dextransucrase Gtf1674 was responsible for synthesizing dextran during soy yogurt fermentation. The produced dextran was mainly composed of α-1,6 glycosidic linkages with a low-branched degree and high molecular weight. After stirring, the dextran entangled with soy protein and formed small aggregates with a dense gel structure and small pores, causing them prone to binding with water and reducing the syneresis. This study suggested the benefits of dextran produced by <em>Leuc. mesenteroides</em> Lm10 in stirred soy yogurt, and facilitated developing the “clean label” plant-derived products.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122948"},"PeriodicalIF":10.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homogeneous synthesis of cationic celluloses with broad-spectrum antibacterial activities for the treatment of vaginitis in mice 均相合成具有广谱抗菌活性的阳离子纤维素，用于治疗小鼠阴道炎

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-05 DOI: 10.1016/j.carbpol.2024.122950

Lian Yang , Lujie Wang , Zihui Zhang , Shufei Zhang , Yong He , Ying Wang , Bingshu Li , Jinping Zhou , Li Hong

{"title":"Homogeneous synthesis of cationic celluloses with broad-spectrum antibacterial activities for the treatment of vaginitis in mice","authors":"Lian Yang , Lujie Wang , Zihui Zhang , Shufei Zhang , Yong He , Ying Wang , Bingshu Li , Jinping Zhou , Li Hong","doi":"10.1016/j.carbpol.2024.122950","DOIUrl":"10.1016/j.carbpol.2024.122950","url":null,"abstract":"<div><div>Microbial infection is a significant health issue for humans. Despite the development of numerous antibiotics, the continuous rise of drug-resistant bacteria highlights the urgent need for new materials to combat these problems. In this study, four water-soluble quaternized cellulose (QC) derivatives with degrees of substitution (DS) ranging from 0.23 to 0.45 were synthesized homogeneously from cellulose carbamate (CC) in NaOH/ZnO aqueous solution. The QC derivatives exhibited broad-spectrum antibacterial activity against gram-negative/positive bacteria, fungi and drug-resistance bacteria. Models of bacterial vaginitis (BV) and vulvovaginal candidiasis (VVC) were used to evaluate the application of QC derivatives visually. Secretion smears and tissue section staining revealed that treatment with QC derivatives led to a reduction in mycelia and spores in the vagina and secretions in the VVC model, along with improved inflammation. In the BV model, vaginal secretions were reduced, clue cells in smears significantly decreased, and inflammation markedly improved. Additionally, cell experiments and staining of mouse organ tissue sections demonstrated that QC derivatives exhibited good biocompatibility. Therefore, using QC derivatives in flushing douches represents a novel approach for treating vaginitis and could serve as a benchmark for addressing other infectious diseases.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122950"},"PeriodicalIF":10.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deferoxamine functionalized alginate-based collagen composite material enhances the integration of metal implant and bone interface 去铁胺功能化藻酸盐胶原蛋白复合材料可增强金属植入物与骨界面的整合性

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-05 DOI: 10.1016/j.carbpol.2024.122944

Zhenjia Che , Xiao Sheng , Qi Sun , Yanglin Wu , Kaihang Song , Aopan Chen , Jing Chen , Qiyun Chen , Ming Cai

{"title":"Deferoxamine functionalized alginate-based collagen composite material enhances the integration of metal implant and bone interface","authors":"Zhenjia Che , Xiao Sheng , Qi Sun , Yanglin Wu , Kaihang Song , Aopan Chen , Jing Chen , Qiyun Chen , Ming Cai","doi":"10.1016/j.carbpol.2024.122944","DOIUrl":"10.1016/j.carbpol.2024.122944","url":null,"abstract":"<div><div>Poor osseointegration markedly compromises the longevity of prostheses. To enhance the stability of titanium implants, surface functionalization is a proven strategy to promote prosthesis-bone integration. This study developed a hydrogel coating capable of simultaneous osteoangiogenesis and vascularization by incorporating deferoxamine (DFO) into a sodium alginate mineralized collagen composite hydrogel. The physicochemical properties of this hydrogel were thoroughly analyzed. <em>In vivo</em> and <em>in vitro</em> experiments confirmed the hydrogel scaffold's osteogenic and angiogenic capabilities. Results indicated that sodium alginate notably enhanced the mechanical characteristics of the mineralized collagen, allowing it to fully infiltrate the interstices of the 3D-printed titanium scaffold. Furthermore, as the hydrogel degraded, collagen, calcium ion, phosphate ion, and DFO were gradually released around the scaffolds, altering the local osteogenic microenvironment and strongly inducing new bone tissue growth. These findings offer novel perspectives for the creation and utilization of functionalized bone implant materials.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122944"},"PeriodicalIF":10.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-responsive gating chitosan-based microcapsules for controlled release of urea fertilizers 用于控制尿素肥料释放的温度响应门控壳聚糖基微胶囊

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-04 DOI: 10.1016/j.carbpol.2024.122929

Nian Xiang , Yuming Lin , Zuzeng Qin , Tongming Su , Xinling Xie , Hongbing Ji

{"title":"Temperature-responsive gating chitosan-based microcapsules for controlled release of urea fertilizers","authors":"Nian Xiang , Yuming Lin , Zuzeng Qin , Tongming Su , Xinling Xie , Hongbing Ji","doi":"10.1016/j.carbpol.2024.122929","DOIUrl":"10.1016/j.carbpol.2024.122929","url":null,"abstract":"<div><div>Polysaccharides-based smart fertilizers are essential for promoting plant growth, yet significant challenges exist in achieving stable structures and synchronizing nutrient release and plant growth. This study developed a temperature-responsive gating chitosan-based microcapsule (CTSMC-g-PNIPAM) by grafting N-isopropyl acrylamide (NIPAM) onto chitosan microcapsules (CTSMC) via atom transfer radical polymerization (ATRP). The interfacial crosslinking of chitosan (CTS) and terephthalendehyde (TPA) formed the CTSMC matrix with a hollow chamber structure and ensured stability. CTSMC-g-PNIPAM exhibited reversible temperature-responsive gating properties and sustained release behavior, and the PNIPAM chains acted like gating switches. Below the low critical solution temperature (LCST) (25 °C), the PNIPAM chains stretched and the gating switch closed, slowing down the release rate. Above the LCST (40 °C), the PNIPAM chains contracted and the gating switch opened, accelerating the release rate. The release behaviors of CTSMC-g-PNIPAM in soil indicated that the gating microcapsule effectively prolonged the release of urea. Moreover, the cumulative release of CTSMC-g-PNIPAM at 40 °C was significantly higher than at 25 °C. The first-order kinetic and Korsmeyer-Peppas model indicate that the urea release involves concentration gradient diffusion and skeleton dissolution. Compared with pure urea, urea@(CTSMC-g-PNIPAM) increased the growth status of maize, suggesting a promising strategy for smart fertilizers.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122929"},"PeriodicalIF":10.7,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan nanoparticles of imatinib mesylate coated with TPGS for the treatment of colon cancer: In-vivo & in-vitro studies 涂有 TPGS 的甲磺酸伊马替尼壳聚糖纳米粒子用于治疗结肠癌：体内和体外研究

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-04 DOI: 10.1016/j.carbpol.2024.122935

Darshan Bhirud , Sankha Bhattacharya , Harshvardhan Raval , Preeti Chidambar Sangave , Girdhari Lal Gupta , Gaurav Paraskar , Megha Jha , Satyam Sharma , Sateesh Belemkar , Devendra Kumar , Rahul Maheshwari , Mayank Sharma

{"title":"Chitosan nanoparticles of imatinib mesylate coated with TPGS for the treatment of colon cancer: In-vivo & in-vitro studies","authors":"Darshan Bhirud , Sankha Bhattacharya , Harshvardhan Raval , Preeti Chidambar Sangave , Girdhari Lal Gupta , Gaurav Paraskar , Megha Jha , Satyam Sharma , Sateesh Belemkar , Devendra Kumar , Rahul Maheshwari , Mayank Sharma","doi":"10.1016/j.carbpol.2024.122935","DOIUrl":"10.1016/j.carbpol.2024.122935","url":null,"abstract":"<div><div>The study aimed to develop and evaluate chitosan-based nanoparticles coated with TPGS for the targeted delivery of imatinib mesylate to colon cancer cells. Particle size and zeta potential analysis were within the acceptable range for targeting colon cancer. CS-IMT-TPGS-NPs had a significant positive zeta potential of 30.4 mV, suggesting improved cellular intake. FE-SEM and TEM demonstrated that the nanoparticles appeared spherical, smooth, and did not aggregate, with a visible TPGS coating. XRD confirmed that crystalline imatinib transitioned to an amorphous state during nano formulation. In-vitro tests on HCT-116 cells demonstrated that CS-IMT-TPGS-NPs outperformed free IMT regarding cytotoxicity, apoptosis induction, cellular uptake, and cell migration inhibition. Additionally, the nanoparticles were examined in vitro using mitochondrial membrane potential, DNA fragmentation, GAPDH relative gene expression, ROS estimation, and cell cycle analysis. The effect of therapy on expected colon-associated bacterial strains was also investigated. The biocompatibility of nanoparticles was assessed by hemolysis and platelet aggregation experiments. The anti-inflammatory impact was determined using the HET-CAM test. Non-Fickian diffusion at pH 5.5 resulted in sustained in-vitro drug release, with no initial burst. In-vivo investigations using albino Wistar rats suggest pharmacokinetic properties for produced nanoparticles, whereas histopathological examinations assess acute toxicity.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122935"},"PeriodicalIF":10.7,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0