Carbohydrate Polymers最新文献_第9页

Acidic dimethyl sulfoxide: A solvent system for the fast dissolution of pectin derivatives suitable for subsequent modification 酸性二甲基亚砜快速溶解果胶衍生物的溶剂系统，适用于后续改性

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-16 DOI: 10.1016/j.carbpol.2024.122872

Hendryk Würfel, Thomas Heinze

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Novel hydrothermal modification to alter functionality and reduce glycemic response of pea starch 改变豌豆淀粉功能和降低血糖反应的新型水热改性技术

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-16 DOI: 10.1016/j.carbpol.2024.122861

Fan Cheng , Gexiao Sun , Xuehong Li , Thomas D. Warkentin , Yongfeng Ai

{"title":"Novel hydrothermal modification to alter functionality and reduce glycemic response of pea starch","authors":"Fan Cheng , Gexiao Sun , Xuehong Li , Thomas D. Warkentin , Yongfeng Ai","doi":"10.1016/j.carbpol.2024.122861","DOIUrl":"10.1016/j.carbpol.2024.122861","url":null,"abstract":"<div><div>Despite being an effective and clean-label method, heat-moisture treatment (HMT) is not commonly used for starch modification in industry due to the difficulty of scale-up. This study aimed to develop a novel method of using extrusion combined with high-temperature drying (EHTD) as an alternative to HMT for starch modification. Pea starch was subjected to extrusion at 37.5 % moisture level and with a low-temperature profile (≤ 65 °C), followed by immediate heating at 130 °C for 1 h. EHTD significantly damaged the granules, altered the X-ray diffraction pattern, and reduced the relative crystallinity of pea starch. Overall, EHTD-modified pea starch exhibited increased gelatinization temperatures and decreased gelatinization enthalpy change, lowered pasting viscosity and gel hardness, as well as enhanced enzymatic resistance than the native pea starch. More importantly, in a human feeding trial (n = 20 healthy participants) to monitor plasma glucose response over a period of 2 h after consuming water-boiled sample (35 g starch, dry basis), EHTD-modified pea starch exhibited 22 % reduction (<em>p</em> < 0.01) in plasma glucose incremental area under the curve as compared to the native counterpart. The results indicated that EHTD could be a new simple and clean-label method to produce functional and low-glycemic starch ingredients.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122861"},"PeriodicalIF":10.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in 3D bioprinted polysaccharide hydrogels for biomedical applications: A comprehensive review 用于生物医学应用的三维生物打印多糖水凝胶的最新进展：全面综述

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-16 DOI: 10.1016/j.carbpol.2024.122845

Fouad Damiri , Ahmed Fatimi , Yang Liu , Adina Magdalena Musuc , André R. Fajardo , B.H. Jaswanth Gowda , Lalitkumar K. Vora , Armin Shavandi , Oseweuba V. Okoro

{"title":"Recent advances in 3D bioprinted polysaccharide hydrogels for biomedical applications: A comprehensive review","authors":"Fouad Damiri , Ahmed Fatimi , Yang Liu , Adina Magdalena Musuc , André R. Fajardo , B.H. Jaswanth Gowda , Lalitkumar K. Vora , Armin Shavandi , Oseweuba V. Okoro","doi":"10.1016/j.carbpol.2024.122845","DOIUrl":"10.1016/j.carbpol.2024.122845","url":null,"abstract":"<div><div>Polysaccharide hydrogels, which can mimic the natural extracellular matrix and possess appealing physicochemical and biological characteristics, have emerged as significant bioinks for 3D bioprinting. They are highly promising for applications in tissue engineering and regenerative medicine because of their ability to enhance cell adhesion, proliferation, and differentiation in a manner akin to the natural cellular environment. This review comprehensively examines the fabrication methods, characteristics, and applications of polysaccharide hydrogel-driven 3D bioprinting, underscoring its potential in tissue engineering, drug delivery, and regenerative medicine. To contribute pertinent knowledge for future research in this field, this review critically examines key aspects, including the chemistry of carbohydrates, manufacturing techniques, formulation of bioinks, and characterization of polysaccharide-based hydrogels. Furthermore, this review explores the primary advancements and applications of 3D-printed polysaccharide hydrogels, encompassing drug delivery systems with controlled release kinetics and targeted therapy, along with tissue-engineered constructs for bone, cartilage, skin, and vascular regeneration. The use of these 3D bioprinted hydrogels in innovative research fields, including disease modeling and drug screening, is also addressed. Despite notable progress, challenges, including modulating the chemistry and properties of polysaccharides, enhancing bioink printability and mechanical properties, and achieving long-term in vivo stability, have been highlighted.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122845"},"PeriodicalIF":10.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation of MeMinD increased amyloplast size with a changed starch granule morphologenesis and structures in cassava storage roots MeMinD 基因突变增加了木薯贮藏根中淀粉粒的大小，并改变了淀粉粒的形态和结构

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-16 DOI: 10.1016/j.carbpol.2024.122884

Xiaohua Lu , Yajie Wang , Mu Pan , Songbi Chen , Ruimei Li , Mengting Geng , Yinhua Chen , Jiao Liu , Jianchun Guo , Yuan Yao

{"title":"Mutation of MeMinD increased amyloplast size with a changed starch granule morphologenesis and structures in cassava storage roots","authors":"Xiaohua Lu , Yajie Wang , Mu Pan , Songbi Chen , Ruimei Li , Mengting Geng , Yinhua Chen , Jiao Liu , Jianchun Guo , Yuan Yao","doi":"10.1016/j.carbpol.2024.122884","DOIUrl":"10.1016/j.carbpol.2024.122884","url":null,"abstract":"<div><div>Amyloplasts are the sites of starch synthesis and accumulation. Little is known about amyloplast division and its effects on the size, structure, and physicochemical properties of starch granules. In this study, we created mutants of plastid division-related gene <em>MeMinD</em> by CRISPR/Cas9 technology, leading to the disruption of normal division of amyloplasts in cassava storage roots. The <em>memind</em> mutants exhibited significantly enlarged amyloplasts with an increased number of starch granules, and broader range of granule sizes. The loss of MeMinD function led to transcriptional reprogramming of gene expressions related to starch-synthesizing enzymes, affecting the fine structure of starch. Starch in <em>memind</em> mutant storage roots showed a significantly decreased proportion of shorter amylopectin chains and an increased proportion of medium and long chains, which ultimately led to a significant increase in apparent amylose content (AAC) in <em>memind</em> mutants compared to that in WT. The changes in starch granule size and structure resulted in a significant increase in onset temperature (To), peak temperature (Tp), and conclusion temperature (Tc) of the gelatinization process, extending the time to reach peak temperature. These data suggest that regulating amyloplast division affects starch accumulation in cassava, presenting an effective strategy for developing novel cassava starch.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122884"},"PeriodicalIF":10.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism underlying V-type structure formation in maize starch through glycerol–ethanol thermal substitution method 甘油-乙醇热置换法在玉米淀粉中形成 V 型结构的机理

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-16 DOI: 10.1016/j.carbpol.2024.122862

Zhijie Zhu , Kai Du , Zhiqian Ma , Xinxin Ma , Xu Chen , Xianfeng Du

{"title":"Mechanism underlying V-type structure formation in maize starch through glycerol–ethanol thermal substitution method","authors":"Zhijie Zhu , Kai Du , Zhiqian Ma , Xinxin Ma , Xu Chen , Xianfeng Du","doi":"10.1016/j.carbpol.2024.122862","DOIUrl":"10.1016/j.carbpol.2024.122862","url":null,"abstract":"<div><div>V-type starches have been widely used in food science, agriculture, and biomedical science, but their mechanism of formation in nonaqueous solvents remains unclear. This study performed glycerol–ethanol thermal substitution to prepare V-type starch at atmospheric pressure. Scanning electron microscopy and confocal laser scanning microscopy revealed that breakage of starch particles began in the hilum region and rapidly spread to the periphery with the temperature increased from 100 °C to 130 °C. The Maltese crosses of the starch disappeared and starch particles had the form of doughnut-shaped rings when the glycerol temperature was 140 °C. Glycerol temperature was not found to significantly affect the chain length of amylopectin, meaning that glycerol molecules may stabilize the conformation of amylose through hydrogen bonding, promoting the amylose to form a V-type spiral structure. Additionally, A-type crystallinity and double helix structure proportion of the samples significantly decreased from 30.81 % and 42.21 % to 5.70 % and 16.47 % as the temperature of glycerol was increased to 140 °C, whereas the V-type crystallinity and single helix structure proportion of the samples remarkably increased from 13.26 % and 1.05 % to 37.97 % and 25.09 %, respectively. This study provided a facile and versatile approach to effectively regulate the formation of V-type starch.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122862"},"PeriodicalIF":10.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MXene and cellulose nanofibers reinforced hydrogel with high strength and photothermal self-healing performances for marine antifouling 具有高强度和光热自修复性能的 MXene 和纤维素纳米纤维增强水凝胶用于海洋防污

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-16 DOI: 10.1016/j.carbpol.2024.122879

Yangkai Xiong , Zhiqiang Fang , Panpan Tang , Yu Li , Hao Jiang , Jipeng Li , Zheng Li , Guoqing Wang

{"title":"MXene and cellulose nanofibers reinforced hydrogel with high strength and photothermal self-healing performances for marine antifouling","authors":"Yangkai Xiong , Zhiqiang Fang , Panpan Tang , Yu Li , Hao Jiang , Jipeng Li , Zheng Li , Guoqing Wang","doi":"10.1016/j.carbpol.2024.122879","DOIUrl":"10.1016/j.carbpol.2024.122879","url":null,"abstract":"<div><div>Biological fouling is a considerable threat to the marine industry because attached microcontaminants and microorganisms inflict serious damage on marine installations and aquacultures. Although there has been tremendous effort in researching marine antifouling, it is yet challenging to develop a long-term and consistent antifouling strategy and materials. Here, polyhexamethylene-biguanide (PHMB) and MXene are introduced into the carboxylated cellulose nanofibers (CNF) reinforced polyvinyl alcohol (PVA) hydrogel to obtain a self-healing and ultra-high toughness antifouling hydrogel. The as-prepared double network (DN) hydrogels have extremely high toughness (16,050 kJ/m<sup>3</sup>), superior anti-protein adhesion (100 %) and anti-algal adhesion (100 %), exceptional antibacterial activity (99.97 %), and remarkable photothermal self-healing capability. Furthermore, the DN hydrogel provide outstanding antifouling performance for at least six months in the actual marine field. This work provides an effective and feasible strategy to prevent marine biofouling and also initiates new approaches to the application of hydrogel.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122879"},"PeriodicalIF":10.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural elucidation of an active arabinoglucan from Gomphrena globosa and its protection effect and mechanism against metabolic dysfunction-associated steatohepatitis 一种来自 Gomphrena globosa 的活性阿拉伯葡聚糖的结构解析及其对代谢功能障碍相关性脂肪性肝炎的保护作用和机制

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-16 DOI: 10.1016/j.carbpol.2024.122860

Meilin Chen , Jianing Zong , Fei He , Wanqi Zhou , Renjie Liu , Haoran Xia , Mengfei Mao , Can Jin , Kaiping Wang , Kan Ding

{"title":"Structural elucidation of an active arabinoglucan from Gomphrena globosa and its protection effect and mechanism against metabolic dysfunction-associated steatohepatitis","authors":"Meilin Chen , Jianing Zong , Fei He , Wanqi Zhou , Renjie Liu , Haoran Xia , Mengfei Mao , Can Jin , Kaiping Wang , Kan Ding","doi":"10.1016/j.carbpol.2024.122860","DOIUrl":"10.1016/j.carbpol.2024.122860","url":null,"abstract":"<div><div>The flower of <em>Gomphrena globosa</em> (<em>G. globosa</em>.) is used as Chinese traditional medicine and functional food. Extractions from <em>G. globosa</em>. have been reported to exert hepatoprotective effects. We further hypothesized that the polysaccharide components, as a bioactive ingredient, might have anti-fatty and hepatitis function. Here, a novel homogeneous arabinoglucan GGL0.05S1 (<em>Mw</em> = 83.9 kDa) from this flower, was characterized by monosaccharide composition analysis, methylation analysis, and NMR. Structural analysis showed that the backbone of GGL0.05S1 consists of →4)-α-Glc<em>p</em>-(1→ and →4,6)-α-Glc<em>p</em>-(1→ residues, and the branched chain linked to the main chain <em>via</em> C-6 of →4,6)-α-Glc<em>p</em>-(1→ in the form α-Ara<em>f</em>-(1→[4)-α-Glc<em>p</em>-(1]<sub>b</sub>→ (b > 2). <em>In vitro</em> experiments demonstrated that GGL0.05S1 could inhibit lipid deposition and ROS overload in free fatty acid-induced hepatocytes, meanwhile <em>in vivo</em> tests showed that GGL0.05S1 effectively protected against liver injury, steatohepatitis, and fibrosis in a CDA-HFD-fed MASH model. Mechanism study further uncovered that GGL0.05S1 augmented the expression and antioxidant ability of thioredoxin protein that ameliorated oxidative stress, promoted fatty acid β-oxidation and mitophagy, up to reducing lipotoxicity and alleviating inflammation <em>via</em> inhibiting NLRP3 signaling pathway. Overall, GGL0.05S1 might be a potential novel active compound with liver-protective effect from <em>G. globosa</em>., and which is informative for anti-MASH new drug development.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122860"},"PeriodicalIF":10.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable self-healing alginate/PEG hydrogels cross-linked via thiol-Michael addition bonds for hemostasis and wound healing 通过硫醇-迈克尔加成键交联的可注射自愈合藻酸盐/聚乙二醇水凝胶用于止血和伤口愈合

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-16 DOI: 10.1016/j.carbpol.2024.122864

Yuxuan Xie , Guichen Li , Jun Wu , Jiachen Zhu , Xuemei Cai , Peizuo Zhao , Dan Zhang , Yuan Zhong

{"title":"Injectable self-healing alginate/PEG hydrogels cross-linked via thiol-Michael addition bonds for hemostasis and wound healing","authors":"Yuxuan Xie , Guichen Li , Jun Wu , Jiachen Zhu , Xuemei Cai , Peizuo Zhao , Dan Zhang , Yuan Zhong","doi":"10.1016/j.carbpol.2024.122864","DOIUrl":"10.1016/j.carbpol.2024.122864","url":null,"abstract":"<div><div>In this study, an alginate/PEG hydrogel was developed via a thiol-Michael addition reaction between oxidized quinone of catechols on dopamine-grafted sodium alginate (SA-DA) and sulfhydryl groups of 4-arm polyethylene glycol tetra-thiol (4-arm PEG-SH) under mildly basic conditions. Through the formation of thiol-terminated catechol groups, the accompanying oxidized catechols are reduced, significantly strengthening the internal network structure of the hydrogel and improving tissue adhesion. Meanwhile, the hydrogels have excellent self-healing properties due to the dynamic non-covalent bonds between the groups. Adjustment of hydrogel properties by varying the mass ratio of two hydrogel precursors. Due to the high content of thiol-terminated catechol groups, the Gel 3 exhibited good tissue adhesion, rapid self-healing ability, and other multifunctions beneficial to wound healing, including killing of <em>E. coli</em> and <em>S. aureus</em>, rapid hemostasis and promoting migration of L929 cells. The full-thickness skin wound model shows that the hydrogel dressing significantly accelerated wound contraction, with increased granulation tissue thickness, collagen disposition, and enhanced vascularization, thus promoting wound healing. Therefore, the thiol-Michael addition reaction is an effective method for creating multifunctional hydrogels, and the injectable self-healing alginate/PEG hydrogels prepared in this way could be used in the biomedical area as wound healing dressing materials.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122864"},"PeriodicalIF":10.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Loss-of-function of SSIIa and SSIIIa confers high resistant starch content in rice endosperm SSIIa 和 SSIIIa 功能缺失可使水稻胚乳中抗性淀粉含量高

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-15 DOI: 10.1016/j.carbpol.2024.122871

Huifang Yang , Xiangbai Dong , Yaru Chai , Shuai Cui , Lihong Tian , Jindan Zhang , Le Qing Qu

{"title":"Loss-of-function of SSIIa and SSIIIa confers high resistant starch content in rice endosperm","authors":"Huifang Yang , Xiangbai Dong , Yaru Chai , Shuai Cui , Lihong Tian , Jindan Zhang , Le Qing Qu","doi":"10.1016/j.carbpol.2024.122871","DOIUrl":"10.1016/j.carbpol.2024.122871","url":null,"abstract":"<div><div>Rice (<em>Oryza sativa</em> L.) endosperm accumulates huge amounts of starch. Rice starch is highly digestible, potentially enhancing the occurrence of blood sugar- and intestine-related diseases such as type 2 diabetes. Resistant starch (RS) is hardly digestible in small intestine but can be converted into beneficial short-chain fatty acids in large intestine, potentially reducing the incidence of these diseases. However, it is still difficult to produce a high RS rice variety. Here, we report that simultaneous deficiency of soluble starch synthases IIa and IIIa confers high RS content in rice endosperm. The <em>ssIIa ssIIIa</em> exhibited higher RS content than did the <em>ssIIIa ssIIIb</em>, a mutant reported currently to have remarkably higher RS content than parental <em>ssIIIa</em>, under our experimental conditions. Loss-of-function of <em>SSIIa</em> and <em>SSIIIa</em> significantly elevated the activity of granule-bound starch synthase I and thus content of amylose. Furthermore, total lipid content increased in mutant seeds, implying that intermediate metabolites spilled out from starch biosynthesis into lipid biosynthesis. The increased amylose content and improved lipid synthesis coordinately contributed to high RS content in mutant seeds. These results further reveal the molecular mechanism of RS occurrence in rice endosperm and provide a critical genetic resource for breeding higher RS rice cultivars.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122871"},"PeriodicalIF":10.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic coating of Laponite swollen-layer and heparin composite for enhanced antifouling and antithrombotic performance 增强防污和抗血栓性能的皂石膨胀层和肝素复合材料协同涂层

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-15 DOI: 10.1016/j.carbpol.2024.122875

Se Youn Jang , Soonjong Roh , Kyungwon Seo , Youngmee Jung , Hai Yen Nguyen Thi , Jeong F. Kim , Hyosung An , Hojeong Jeon , Il Keun Kwon , Jin Yoo

{"title":"Synergistic coating of Laponite swollen-layer and heparin composite for enhanced antifouling and antithrombotic performance","authors":"Se Youn Jang , Soonjong Roh , Kyungwon Seo , Youngmee Jung , Hai Yen Nguyen Thi , Jeong F. Kim , Hyosung An , Hojeong Jeon , Il Keun Kwon , Jin Yoo","doi":"10.1016/j.carbpol.2024.122875","DOIUrl":"10.1016/j.carbpol.2024.122875","url":null,"abstract":"<div><div>When implantable medical devices come into contact with blood, acute thrombosis and inflammation often occur due to the adhesion and denaturation of plasma proteins, ultimately degrading device performance. To address this issue, we developed a gel-like coating with superhydrophilic properties, incorporating a heparin based outer layer designed to minimize foreign body reactions and enhance hemocompatibility. The coating was engineered using a layer-by-layer (LbL) assembly of poly-<span>l</span>-lysine (PLL), laponite (Lap), and heparin (Hep), utilizing electrostatic interactions. The successful formation of stable layers was confirmed by QCM-D analysis. The inner layer, composed of PLL/Lap multilayers, formed a gel-like structure that maintained superhydrophilicity and effectively prevented cell adhesion. The outermost PLL/Hep multilayers significantly suppressed thrombus formation by inhibiting plasma protein and red blood cell adsorption as well as platelet activation. The coating exhibited excellent stability, retaining its superhydrophilic properties and heparin functionality even after 7 days of immersion in DPBS. Additionally, in vitro hemocompatibility and cytocompatibility tests confirmed its non-toxicity and overall biocompatibility. These results highlight the potential application of this coating to various implantable medical devices, providing a robust solution for improving device performance and safety.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122875"},"PeriodicalIF":10.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0