Carbohydrate Polymers最新文献_第8页

Effects of aging on the fine structure, chain conformation, and morphology of Chenpi polysaccharides 老化对陈皮多糖精细结构、链构象和形态的影响

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-13 DOI: 10.1016/j.carbpol.2024.122970

Gang Xu, Jingyun Zhao, Jieqiong Yao, Yang Xu, Xianghao Yuan, Siyi Pan

{"title":"Effects of aging on the fine structure, chain conformation, and morphology of Chenpi polysaccharides","authors":"Gang Xu, Jingyun Zhao, Jieqiong Yao, Yang Xu, Xianghao Yuan, Siyi Pan","doi":"10.1016/j.carbpol.2024.122970","DOIUrl":"10.1016/j.carbpol.2024.122970","url":null,"abstract":"<div><div>The aging process endows Chenpi (<em>Pericarpium Citri Reticulatae</em>) with unique value and efficacy. This study investigated the dynamic changes in the fine structure, chain conformation, and morphology of water-soluble polysaccharides from Chenpi over time. In the initial storage period of Chenpi (1 year), Chenpi polysaccharides (CP) exhibited a triple-helical structure, with chains entangled and aggregated into rough spherical conformations. Galacturonic acid (GalA, 47.78 mol%) was the predominant monosaccharide. As aging progressed, the homogalacturonan (HG) regions gradually degraded, leading to decreases in molecular sizes (particle size, M<sub>w</sub>, M<sub>n</sub>, and R<sub>g</sub>), a reduction in the degree of esterification, and a weakening of the semicrystalline structure. Consequently, arabinose (Ara) emerged as the main monosaccharide (41.38 mol%). Neutral sugars continuously enriched the rhamnogalacturonan (RG-I) side chains, forming highly branched single molecules that curled into spherical shapes. By 15 years, CP were fully degraded, adopting a compact molecular conformation with the triple-helix structure disappearing and sizes uniformly below 20 nm. However, AFM results indicated aggregation phenomena in 15-year CP. Additionally, CP viscosity decreased while thermal stability improved, reflecting the natural structural transformation of CP. This study provides scientific evidence supporting the application of Chenpi in the food and pharmaceutical industries.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122970"},"PeriodicalIF":10.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial chitosan/organic rectorite nanocomposite-conjugated gelatin/β-cyclodextrin hydrogels with improved hemostasis performance for wound repair 具有更好止血性能的抗菌壳聚糖/有机雷克托石纳米复合-共轭明胶/β-环糊精水凝胶用于伤口修复

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-13 DOI: 10.1016/j.carbpol.2024.122961

Rong Huang , Yongqian Bian , Wenxuan Wang , Lirong Xu, Hao Zhang, Haowei Zhou, Jincheng Du, Jiaqi Li, Yuheng Zhang, Xueyong Li, Jing Li

{"title":"Antibacterial chitosan/organic rectorite nanocomposite-conjugated gelatin/β-cyclodextrin hydrogels with improved hemostasis performance for wound repair","authors":"Rong Huang , Yongqian Bian , Wenxuan Wang , Lirong Xu, Hao Zhang, Haowei Zhou, Jincheng Du, Jiaqi Li, Yuheng Zhang, Xueyong Li, Jing Li","doi":"10.1016/j.carbpol.2024.122961","DOIUrl":"10.1016/j.carbpol.2024.122961","url":null,"abstract":"<div><div>Bacterial infections and severe bleeding continue to pose significant challenges in wound repair. There is an urgent need for innovative, nature-inspired hydrogel dressings with antibacterial and hemostatic properties. A Ge-β-CD-CS-OREC conjugate hydrogel was developed by grafting β-CD and CS-OREC nanocomposites into a Ge matrix using EDC/NHS crosslinking, as confirmed by FT-IR and EDX analyses. Compared to single Ge-β-CD cross-linked hydrogels, the addition of CS-OREC enhanced the hydrogel's properties, including increased pore size (60 ± 14 μm), improved wettability (WCA = 28.82 ± 0.6°), enhanced tensile strength (41.3 ± 3.56 KPa), and strong tissue adhesion. Furthermore, this hydrogel demonstrated excellent cytocompatibility when co-cultured with keratinocytes (Kcs) and vascular endothelial cells (VECs). The incorporation of CS chains into OREC interlayers allowed the hydrogel to specifically target bacteria and increase membrane permeability in <em>Pseudomonas aeruginosa</em> (PA), <em>Klebsiella pneumoniae</em> (KP), and <em>Staphylococcus aureus</em> (SA), effectively reducing the bacterial load in infected wounds by 50.24–73.92 % compared to controls in vivo<em>.</em> Further, the hydrogel exhibited superior hemostatic efficiency (78 ± 10 s) over commercial gauze and other gels by enhancing platelet activation and coagulation factor secretion. The hydrogel accelerated tissue regeneration by promoting epithelial maturation and blood vessel regeneration, indicating its clinical potential as promising wound dressing.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122961"},"PeriodicalIF":10.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular structure of enzyme-synthesized amylose-like chimeric isomaltomegalosaccharides and their encapsulation of the sulfasalazine prodrug 酶合成的直链淀粉样嵌合异麦芽糖的分子结构及其对磺胺沙拉嗪原药的包裹作用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-13 DOI: 10.1016/j.carbpol.2024.122956

Weeranuch Lang , Yoshiaki Yuguchi , Chun-Yao Ke , Ting-Wei Chang , Yuya Kumagai , Wilaiwan Kaenying , Takayoshi Tagami , Feng Li , Takuya Yamamoto , Kenji Tajima , Kenji Takahashi , Takuya Isono , Toshifumi Satoh , Atsuo Kimura

{"title":"Molecular structure of enzyme-synthesized amylose-like chimeric isomaltomegalosaccharides and their encapsulation of the sulfasalazine prodrug","authors":"Weeranuch Lang , Yoshiaki Yuguchi , Chun-Yao Ke , Ting-Wei Chang , Yuya Kumagai , Wilaiwan Kaenying , Takayoshi Tagami , Feng Li , Takuya Yamamoto , Kenji Tajima , Kenji Takahashi , Takuya Isono , Toshifumi Satoh , Atsuo Kimura","doi":"10.1016/j.carbpol.2024.122956","DOIUrl":"10.1016/j.carbpol.2024.122956","url":null,"abstract":"<div><div>The glucoconjugation between linear chimeric α-(1→4)- and α-(1→6)-glucosidic segments exhibits functional properties throughout their structure. In this study, we enzymatically synthesized three new series of chimeric nonreducing isomaltomegalosaccharides (N-IMS-<em>n</em>/<em>m</em>), each featuring a constant <em>n</em>, α-(1→4)-segment (average degree of polymerization, DP = 22–25) at the nonreducing terminal, and varying <em>m</em>, α-(1→6)-main chain lengths (DP = 7–53). The synthesized compounds—N-IMS-25/7, N-IMS-24/19, and N-IMS-22/53—were compared to amylose (DP = 28) and previous samples of N-IMS-15/35 and D-IMS-28.3/13/3. D-IMS refers to a sugar with double α-(1→4)-segments at both the nonreducing and reducing ends. The binding affinity to the aromatic prodrug sulfasalazine (SZ) was assessed using a phase-solubility assay, followed by freeze-thawing. Wide-angle X-ray scattering revealed B-type crystalline patterns in bulk, and the crystallinity generally reduced with the increasing α-(1→6) segment. Interestingly, the B-type crystal structure was maintained even after SZ encapsulation, in contrast to the more common transition to V-type crystals upon drug encapsulation. Multi-angle dynamic light scattering and small-angle X-ray scattering revealed an intricate solution-state morphology, both in the absence and presence of SZ. Glucoconjugation aids in maintaining structural organization and integrity, even after the incorporation of the large SZ molecule.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122956"},"PeriodicalIF":10.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polysaccharide-based hydrogels for atopic dermatitis management: A review 多糖类水凝胶治疗特应性皮炎：综述

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-13 DOI: 10.1016/j.carbpol.2024.122966

Harinash Rao, Joash Ban Lee Tan

{"title":"Polysaccharide-based hydrogels for atopic dermatitis management: A review","authors":"Harinash Rao, Joash Ban Lee Tan","doi":"10.1016/j.carbpol.2024.122966","DOIUrl":"10.1016/j.carbpol.2024.122966","url":null,"abstract":"<div><div>Atopic dermatitis (AD) is the most common form of eczema and the most burdensome skin disease globally, affecting nearly 223 million. A major AD predisposition is genetic susceptibility, affecting skin barrier integrity and cell-mediated immunity. Manifesting as red, dry, and itchy skin, basic treatment involves skin hydration with emollients. Despite their effectiveness, poor patient compliance remains a major drawback. In severe cases, medicated emollients are used, but carry risks, including skin thinning, and immunosuppression. Hence, hydrogels have emerged as a promising alternative for AD management based on their ability to improve skin hydration, attributed to their hydrophilicity and high water retention capacity. Moreover, researchers have loaded hydrogels with various compounds for AD management; they also hold the potential to reduce systemic side effects of commercial drugs by enhancing dermal retention. Hydrogels address the challenges of patient compliance based on their non-greasy texture and reduced application frequency. Their appeal also stems from their versatility, as they can be fabricated from varying polymers. Due to their abundance, this review focuses on polysaccharides including alginate, cellulose, chitosan, and hyaluronic acid, which are preferred for fabricating natural and modified natural hydrogels for AD. It also briefly explores hydrogel application methods and key AD models.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122966"},"PeriodicalIF":10.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guest inclusion by native cyclodextrins in solid state and solutions: A review 原生环糊精在固态和溶液中的客体包含：综述

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-13 DOI: 10.1016/j.carbpol.2024.122962

Askar K. Gatiatulin, Marat A. Ziganshin, Valery V. Gorbatchuk

{"title":"Guest inclusion by native cyclodextrins in solid state and solutions: A review","authors":"Askar K. Gatiatulin, Marat A. Ziganshin, Valery V. Gorbatchuk","doi":"10.1016/j.carbpol.2024.122962","DOIUrl":"10.1016/j.carbpol.2024.122962","url":null,"abstract":"<div><div>In many industrial applications, preparation of cyclodextrin (CD) inclusion complexes with drugs, food additives, dyes and components of essence oils is performed in solid mixtures, slurries or paste-like systems having lack of water to dissolve cyclodextrin and guest completely. Such systems need a different description than supplied by classical analysis of CD complexation in aqueous solutions. The main feature of solid-state guest inclusion is the phase transition from solid CD to solid inclusion compound. This implies a complex interplay between a size exclusion effect for guest inclusion, a cooperative activation of this process by the third component such as water or organic compound and competition of guest and water for the space inside CD crystal lattice. The present review summarizes the current state of research of guest inclusion by native CDs in solid state and compares the driving forces of this process and its structure-property relationships with those of complexation in aqueous solutions. For an adequate comparison, the latter process was analyzed in thermodynamic activity scale, which allowed to separate hydrophobic effect and such important factors of complex stability as guest molecular shape and “high-energy” water.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122962"},"PeriodicalIF":10.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sheet-laminated additive manufacturing of bacterial cellulose nanofiber-reinforced hydrogels 细菌纤维素纳米纤维增强水凝胶的片状层压添加制造

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-13 DOI: 10.1016/j.carbpol.2024.122972

Junsik Choi , Kiho Sung , Jinho Hyun , Sungchul Shin

{"title":"Sheet-laminated additive manufacturing of bacterial cellulose nanofiber-reinforced hydrogels","authors":"Junsik Choi , Kiho Sung , Jinho Hyun , Sungchul Shin","doi":"10.1016/j.carbpol.2024.122972","DOIUrl":"10.1016/j.carbpol.2024.122972","url":null,"abstract":"<div><div>Three-dimensional (3D) printing of hydrogels offers promising potential for creating intricate, customizable structures with superior elasticity, softness, and biocompatibility. However, due to their high-water content, hydrogels often suffer from reduced mechanical strength, which is further decreased when they absorb water, limiting their use in environments requiring high mechanical durability. To address this, we developed a novel 3D printing technique to fabricate bacterial cellulose (BC) nanofiber-reinforced hydrogels, which we term sheet-laminated additive manufacturing (SLAM). SLAM is based on digital light processing (DLP) 3D printing technology and involves a process of sequentially layering BC nanofiber sheets impregnated with a photocrosslinkable monomer. The BC nanofiber sheets provide a unique 3D network, resulting in a significant enhancement of the mechanical strength of various photocrosslinkable hydrogels. A unique aspect of BC sheets is their ability to further improve mechanical properties by inducing nanofiber alignment or adjusting nanofiber density through stretching and compression pretreatments. The printed BC nanofiber-reinforced hydrogels maintained their strength after swelling and demonstrated exceptional performance in applications requiring high mechanical robustness. Our SLAM approach successfully created complex 3D structures, such as BC-reinforced hydrogel earthworm structures and pressure sensors, demonstrating its potential for advanced applications in high-stress environments.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122972"},"PeriodicalIF":10.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and physicochemical properties of corn starch modified by phosphorylase b, hexokinase and alkaline phosphatase 经磷酸化酶 b、己糖激酶和碱性磷酸酶改性的玉米淀粉的结构和理化特性

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-13 DOI: 10.1016/j.carbpol.2024.122979

Shuo Sun , Dengyue Sun , Li Guo , Bo Cui , Feixue Zou , Jinpeng Wang , Chunrui Sun , Yu Zhu , Xueling Li

{"title":"Structural and physicochemical properties of corn starch modified by phosphorylase b, hexokinase and alkaline phosphatase","authors":"Shuo Sun , Dengyue Sun , Li Guo , Bo Cui , Feixue Zou , Jinpeng Wang , Chunrui Sun , Yu Zhu , Xueling Li","doi":"10.1016/j.carbpol.2024.122979","DOIUrl":"10.1016/j.carbpol.2024.122979","url":null,"abstract":"<div><div>To improve the functional properties of corn starch, phosphorylase <em>b</em> (PB), hexokinase (HK), and alkaline phosphatase (AP) were used to produce enzyme-modified starches (PBMS, HKMS, and APMS). The results showed that enzyme-modified starches had different phosphorus contents and degrees of substitution. The presence of P<img>O bonds and P-O-C bonds further demonstrated that phosphate groups were grafted into starch. The proportions of monostarch phosphate in PBMS, HKMS, and APMS were 77.05 %, 79.33 % and 85.88 %, respectively. The introduction of phosphate groups affected the functional properties of starch. The swelling powers of PBMS, HKMS and APMS increased from 0.99 % to 12.86 %, 10.83 % and 5.95 %, respectively. Compared to native starch (1820 mPa·s), the peak viscosities of PBMS, HKMS and APMS increased to 2655, 2838, and 2021 mPa·s, respectively. Meanwhile, the introduction of phosphate groups endowed phosphorylated starch with better freeze-thaw stability, larger paste transparency, higher solubility, and slower retrogradation rate.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122979"},"PeriodicalIF":10.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis of Aureobasidium pullulans BL06 and identification of key factors affecting pullulan production Aureobasidium pullulans BL06 的转录组分析以及影响乌拉坦生产的关键因素的鉴定

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-13 DOI: 10.1016/j.carbpol.2024.122984

Jiaqi Gao , Xiaoping Liao , Hongwu Ma , Wenqin Bai

{"title":"Transcriptome analysis of Aureobasidium pullulans BL06 and identification of key factors affecting pullulan production","authors":"Jiaqi Gao , Xiaoping Liao , Hongwu Ma , Wenqin Bai","doi":"10.1016/j.carbpol.2024.122984","DOIUrl":"10.1016/j.carbpol.2024.122984","url":null,"abstract":"<div><div>Pullulan, a versatile water-soluble polysaccharide, is widely used across various industries. To minimize byproduct interference, <em>Aureobasidium pullulans</em> BL06Δ<em>PMAs</em> was engineered, resulting in a higher yield and a lower molecular weight (MW) than the parent strain <em>A. pullulans</em> BL06. Comparative transcriptomic analysis revealed differentially expressed genes (DEGs) involved in sucrose metabolism, gluconeogenesis, glyoxylate metabolism, and amino acid metabolism. These DEGs may influence substrate consumption, energy production, and membrane composition, ultimately impacting pullulan synthesis. Additionally, further experimental validations were conducted on the genes with the most significant differential expression. Overexpressing glycosyltransferase gene (<em>gta1</em>, the third most differentially expressed gene) in <em>A. pullulans</em> BL06 increased pullulan production by 8.1 %, indicating its role in short α-1,4-glucan synthesis. Overexpression of the transmembrane transporter gene (<em>st1,</em> the most significantly differentially expressed gene) reduced pullulan molecular weight by 25 %, which potentially influences cell membrane fluidity and pullulan secretion. Furthermore, amylase (Amy1) was found to significantly impact molecular weight (MW) within the first 48 h of fermentation, an effect not previously reported for amylase, while its knockout resulted in a remarkable 7.6-fold increase in pullulan MW. These findings provide valuable insights for regulating pullulan yield and MW, offering innovative genetic targets for strains engineering in future industrial applications.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122984"},"PeriodicalIF":10.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyaluronic acid-based minocycline-loaded dissolving microneedle: Innovation in local minocycline delivery for periodontitis 基于透明质酸的米诺环素溶解微针：治疗牙周炎的局部米诺环素给药创新

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-12 DOI: 10.1016/j.carbpol.2024.122976

Young Woo Song , Jeehye Nam , Jeongin Kim , Youjin Lee , Jaibyung Choi , Hye Su Min , Huisuk Yang , Yoeseph Cho , Sungmin Hwang , Junghyun Son , Ui-Won Jung , Hyungil Jung

{"title":"Hyaluronic acid-based minocycline-loaded dissolving microneedle: Innovation in local minocycline delivery for periodontitis","authors":"Young Woo Song , Jeehye Nam , Jeongin Kim , Youjin Lee , Jaibyung Choi , Hye Su Min , Huisuk Yang , Yoeseph Cho , Sungmin Hwang , Junghyun Son , Ui-Won Jung , Hyungil Jung","doi":"10.1016/j.carbpol.2024.122976","DOIUrl":"10.1016/j.carbpol.2024.122976","url":null,"abstract":"<div><div>Periodontitis is a prevalent inflammatory disease that affects tooth-supporting tissues and is induced by complex polymicrobial dental plaques. Prior treatments, including topical antibiotic ointments, have faced difficulties in tissue permeability issues. Although dissolving microneedle (DMN) has been proposed as a painless and highly efficient transdermal drug delivery system to resolve this challenge, minocycline, widely used for the treatment of periodontitis, is light-sensitive, making it challenging to maintain its stability using conventional fabrication methods. Our hyaluronic acid-based minocycline-loaded dissolving microneedle (HAM-DMN) was designed utilizing an innovative light-blocking strategy, preserving 94.4 % of minocycline's stability, as confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. HAM-DMNs demonstrated antimicrobial efficacy in <em>in vitro</em> zone of inhibition tests with <em>Streptococcus mutans</em> strains and provided enhanced local delivery of minocycline to porcine oral gingival mucosa at concentrations 6.1 times higher than those of commercial ointments. <em>In vivo</em> studies in periodontitis-induced rat models showed that HAM-DMNs reduced levels of junctional epithelium more effectively than control and blank DMN groups, indicating enhanced treatment efficacy. HAM-DMN is a novel local delivery system developed to overcome the limitations of systemic delivery and conventional topical treatment. We suggest that HAM-DMNs can replace injections for the treatment of intraoral mucosal and systemic diseases.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122976"},"PeriodicalIF":10.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intervention mechanism of amphiphilic natural sweeteners on starch chain dynamic behavior: Computational and experimental insights 两亲性天然甜味剂对淀粉链动态行为的干预机制：计算和实验见解

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-11-12 DOI: 10.1016/j.carbpol.2024.122978

Fan Wang, Jianfu Shen, Baiyi Lu

{"title":"Intervention mechanism of amphiphilic natural sweeteners on starch chain dynamic behavior: Computational and experimental insights","authors":"Fan Wang, Jianfu Shen, Baiyi Lu","doi":"10.1016/j.carbpol.2024.122978","DOIUrl":"10.1016/j.carbpol.2024.122978","url":null,"abstract":"<div><div>Amphiphilic natural sweeteners (i.e. steviol glycosides (STE) and glycyrrhizic acid (GA)) have been adopted to improve the quality of various starchy products, which can fundamentally be characterized as the intervention of the former in the chain dynamic behavior of the latter. However, these phenomena and related mechanisms still lack systematic insights. Herein, dual-temperature molecular dynamic simulations combined with experimental analysis were used to tandemly investigate the intervention of sweeteners in six types of chain dynamic behaviors that are strongly correlated with starch properties, including unwinding, movement, long/short-term reassociation, rearrangement, and depolymerization. The results show that STE and GA both promoted the chain unwinding and movement, and also retarded the chain short/long-term reassociation and rearrangement. Besides, GA exhibited a greater role than STE in facilitating chain unwinding and movement. Peculiarly, GA (0 %–40 % w/w) collaborated with starch to form a new microstructure, especially at high content (≥ 20 % w/w), which endowed starch with exceptionally high hardness (15.50 gf→189.36 gf) and hardening rate (2.72 gf/d→17.76 gf/d), and also placed a physical barrier to retard starch depolymerization (slowly digestible starch: 11.26 %→20.62 %). This work contributes data and theoretical support for the development of starch/amphiphilic natural sweetener composite matrices.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122978"},"PeriodicalIF":10.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0