Zexie Tang Polysaccharides (ZXTPs) alleviate high-fat diet-induced NAFL in mice through improving intestinal microbiota imbalance and liver metabolic dysfunction

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-06-30 DOI:10.1016/j.carbpol.2025.123937

Zhiqiang Zhang , Yanle Zhi , Shengchao Wang , Fengjiao Zhang , Sufeng Zhang , Jiarui Wu

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引用次数: 0

Abstract

Zexie Tang (Alisma Decoction, ZXT), derived from “Jin-Guiyaolue” (Synopsis of Prescriptions from the Golden Chamber), is composed of two kinds of herbs, namely Zexie (Atractylodes macrocephala) and Baizhu (Atractylodes macrocephala). And Baizhu (Atractylodes macrocephala), and its extractions have demonstrated hepatoprotective properties. We hypothesize that the polysaccharides in Zexie Tang might have therapeutic effects on Non-Alcoholic Fatty Liver Disease (NAFLD). In this study, we isolated a novel homogeneous polysaccharide, Zexie Tang Polysaccharides (ZXTPs), and its structure was elucidated by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectra. The main chain of the polysaccharide is →1-β-D-Fruf-2 → 1-β-D-Fruf-2 →, and the structural formula of the polysaccharide can be deduced as follows: α-D-Glcp-1 → [1-β-D-Fruf-2]₁₁ → 1-β-D-Fru. In vivo experiments indicated that ZXTPs could significantly reduce hepatic steatosis, fasting triglyceride, and cholesterol levels in NAFLD mice. Cell proliferation and Oil Red O staining results showed that ZXTPs concentration-dependently increased cell viability and significantly improved lipid metabolism in vitro. Mechanistically, ZXTPs markedly inhibited the formation of lipid both in vitro and in vivo through activating Autophage and AMPK/PPAR-α pathways. In this study, ZXTPs exert beneficial effects against NAFLD through dual mechanisms involving metabolic regulation and intestinal microbiome modification.

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泽泻汤多糖（ZXTPs）通过改善肠道菌群失衡和肝脏代谢功能紊乱来缓解高脂饮食诱导的小鼠NAFL

泽泻汤（泽泻汤，ZXT），源自《金匮要略》（《金匮要略》），由两种草药组成，即泽泻（苍术）和白术（苍术）。白术及其提取物具有保肝作用。我们推测泽泻汤多糖可能对非酒精性脂肪肝（NAFLD）有治疗作用。本研究分离了一种新的均相多糖——泽泻汤多糖（ZXTPs），并通过单糖组成、甲基化分析、部分酸水解和核磁共振波谱对其结构进行了鉴定。多糖的主链为→1-β-D-Fruf-2→1-β-D-Fruf-2→，可推导出多糖的结构式为：α-D-Glcp-1→[1-β-D-Fruf-2]11→1-β-D-Fru。体内实验表明，ZXTPs能显著降低NAFLD小鼠肝脏脂肪变性、空腹甘油三酯和胆固醇水平。细胞增殖和油红O染色结果显示，ZXTPs浓度依赖性地提高了细胞活力，显著改善了体外脂质代谢。在体外和体内，ZXTPs通过激活Autophage和AMPK/PPAR-α途径显著抑制脂质形成。在本研究中，zxtp通过代谢调节和肠道微生物组修饰的双重机制对NAFLD发挥有益作用。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.