Carbohydrate Polymers最新文献_第2页

Hypolipidemic activity and mechanisms of carboxymethyl pachymaran: Impact of the degree of substitution 羧甲基茯苓多糖的降血脂活性和机制：取代度的影响

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-12 DOI: 10.1016/j.carbpol.2025.123890

Lina Pei , Xi Feng , Lijia Zhang , Wen Huang , Zhinan Mei , Ying Liu

{"title":"Hypolipidemic activity and mechanisms of carboxymethyl pachymaran: Impact of the degree of substitution","authors":"Lina Pei , Xi Feng , Lijia Zhang , Wen Huang , Zhinan Mei , Ying Liu","doi":"10.1016/j.carbpol.2025.123890","DOIUrl":"10.1016/j.carbpol.2025.123890","url":null,"abstract":"<div><div>In this study, carboxymethyl pachymaran (CMP) with three different degrees of substitution were prepared and their hypolipidemic activities were investigated. CMP2 with a substitution degree of 0.791 had different molecular weight and surface smoothness textural profile than CMP1 (0.756) and CMP2 (0.871). All CMPs could reduce 0.5 mM oleic acid-induced lipid accumulation in HepG2 cells, however, CMP2 exhibited a stronger activity than that of CMP1 and CMP3 in lowering lipid and oxidative stress levels. The results demonstrated that CMP2 can reduce lipid levels, serum inflammatory factors and improve liver enzymes in mice. Moreover, the gut microbiota composition was balance and the levels of healthy beneficial short-chain fatty acids were increased treated by CMP2. RT-qPCR analysis demonstrated that CMP2 regulated hepatic and biliary cholesterol efflux by up-regulating the expression levels of LDLR, PPARα, and ABCA1, while down-regulating the expression levels of SREBP-2. CMP2 effectively inhibited lipid accumulation in hepatocytes and attenuates inflammatory injury by a dual regulatory mechanism. Our results revealed potential to develop hypolipidemic functional foods with CMP2.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123890"},"PeriodicalIF":10.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermoresponsive hydrogelation of N-acetyl chitohexaose: Gelation mechanism and application in controlled protein release n -乙酰基壳聚糖酶的热响应性凝胶化：凝胶化机理及其在蛋白质控制释放中的应用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-11 DOI: 10.1016/j.carbpol.2025.123897

Hiroyuki Kono , Yuya Nagaoka , Ayumu Izutsu , Shunsuke Kita , Katsumi Maenaka , Shunsuke Nishio , Takeshi Hattori , Yuka Yoshikawa , Kousuke Michishita , Taichi Usui , Takuya Isono , Makoto Ogata

{"title":"Thermoresponsive hydrogelation of N-acetyl chitohexaose: Gelation mechanism and application in controlled protein release","authors":"Hiroyuki Kono , Yuya Nagaoka , Ayumu Izutsu , Shunsuke Kita , Katsumi Maenaka , Shunsuke Nishio , Takeshi Hattori , Yuka Yoshikawa , Kousuke Michishita , Taichi Usui , Takuya Isono , Makoto Ogata","doi":"10.1016/j.carbpol.2025.123897","DOIUrl":"10.1016/j.carbpol.2025.123897","url":null,"abstract":"<div><div>Chitin is a polysaccharide composed of <em>N</em>-acetylglucosamine units that form highly crystalline structures in biological systems; however, the precise mechanism underlying its self-assembly in aqueous environments remains unclear. In this study, we demonstrated that <em>N</em>-acetyl chitohexaose (GN6), a chitin oligosaccharide with a degree of polymerization of six, uniquely undergoes thermally induced self-assembly into crystalline nanofibers and forms reversible hydrogels in water. Among chitin oligosaccharides ranging from monomer to hexamer, only GN6 exhibited gelation upon heating, which is attributed to its optimal molecular length and balanced hydrophilic–hydrophobic characteristics. Comprehensive structural analyses using solid-state nuclear magnetic resonance spectroscopy, small- and wide-angle X-ray scattering, transmission electron microscopy, atomic force microscopy, and microcrystal electron diffraction revealed that GN6 molecules align along the crystallographic <em>β</em>-axis and assemble into nanofibers with highly ordered α-chitin-type molecular packing. This directional organization is driven by cooperative hydrogen bonding and hydrophobic interactions. Furthermore, the hydrogels exhibited a sustained release of encapsulated proteins, including insulin, albumin, and immunoglobulin G, for more than four weeks under physiological conditions, without using chemical crosslinking agents. These findings highlight the potential of this oligosaccharide as a biocompatible, drug delivery material and as a minimalistic model system for studying polysaccharide self-assembly in aqueous environments.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123897"},"PeriodicalIF":10.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A light-controlled dextran nano-immunomodulator amplifies immunogenic cell death for cancer immunotherapy 一种光控右旋糖酐纳米免疫调节剂用于癌症免疫治疗的免疫原性细胞死亡放大

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-11 DOI: 10.1016/j.carbpol.2025.123885

Danni Xu, Jingxuan Ju, Wenchao Chen, Jiaqian Miao, Sisi Chen, Junjie Wang, Li Xu, Guangbo Ge, Hongping Deng

{"title":"A light-controlled dextran nano-immunomodulator amplifies immunogenic cell death for cancer immunotherapy","authors":"Danni Xu, Jingxuan Ju, Wenchao Chen, Jiaqian Miao, Sisi Chen, Junjie Wang, Li Xu, Guangbo Ge, Hongping Deng","doi":"10.1016/j.carbpol.2025.123885","DOIUrl":"10.1016/j.carbpol.2025.123885","url":null,"abstract":"<div><div>Immunogenic cell death (ICD) is a programmed modality of regulated cell death that induces strong adaptive immune responses, which is severely related to the drug release kinetics of nanotherapeutics. Here, we report a light-controlled polysaccharide nano-immunomodulator (Dex-NB-CPT) with enhanced ICD induction for cancer immunotherapy. Dex-NB-CPT does not self-assemble into nanoparticles as a single-molecular micelle, and releases ICD inducer camptothecin (CPT) rapidly via a photocleavable linker under light illumination, avoiding the hindrance of nanoparticle disassembly and physical encapsulation to realize efficient drug release. Upon light illumination, the cell apoptosis of 4T1 and B16F10 cells was evaluated as compared with CPT and Dex-NB-CPT without light illumination. The ICD induction efficiency was also augmented after light illumination as validated by increased cellular calreticulin (CRT) and high mobility group protein B1 (HMGB1) levels, which further promoted macrophage M1 polarization and dendritic cell maturation. In a 4T1 tumor-bearing mice, Dex-NB-CPT with light illumination significantly inhibited 4T1 tumor growth by inducing robust ICD effect with much higher levels of CRT and HMGB1 to activate dendritic cells and cytotoxic T cells for enhanced cancer immunotherapy. Our work provides a robust strategy to potentiate cancer immunotherapy by augmenting ICD effect with a light-controlled polysaccharide nano-immunomodulator.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123885"},"PeriodicalIF":10.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sodium alginate/carboxymethyl chitosan/gelatin-naringenin pH-responsive hydrogel beads for oral delivery of traditional Chinese herbal medicines 中药口服给药用海藻酸钠/羧甲基壳聚糖/明胶柚皮素ph响应水凝胶珠

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-11 DOI: 10.1016/j.carbpol.2025.123870

Zixing Yan , Qingling Nie , Jiajia Liu , Jingxin Chen , Youmei Liu , Yudong Lu , Mengjun Xu , Zhenwen Lin

{"title":"Sodium alginate/carboxymethyl chitosan/gelatin-naringenin pH-responsive hydrogel beads for oral delivery of traditional Chinese herbal medicines","authors":"Zixing Yan , Qingling Nie , Jiajia Liu , Jingxin Chen , Youmei Liu , Yudong Lu , Mengjun Xu , Zhenwen Lin","doi":"10.1016/j.carbpol.2025.123870","DOIUrl":"10.1016/j.carbpol.2025.123870","url":null,"abstract":"<div><div>Stomach disorders are a common category of digestive system diseases. With the increasing emphasis on traditional medicine, the use of herbal medicines in treating gastric diseases has attracted much attention. However, direct administration of herbal medicines with short half-life leads to low drug utilization. To address this problem, pH-responsive composite hydrogel beads consisting of sodium alginate/carboxymethyl chitosan/gelatin-naringenin (SA/CMCh/Gel-Nar) were designed in this study to encapsulate decocted herbal saddle decoctions (AZD) for use as sustained-release medications for gastrointestinal disorders. The SA/CMCh/Gel-Nar hydrogel beads have high drug loading capacity, strong adhesion (98.33 % retention), and excellent pH-responsive sustained-release properties. In SGF, 39 % of the drug was released within 2 h, ensuring rapid therapeutic efficacy, while in SIF, the remaining 61 % was gradually released within 23 h. With excellent antimicrobial efficacy (>93.4 %) and high biosafety (cell viability >97.21 %), these hydrogel beads provide a promising and safe platform for sustained drug delivery in the gastrointestinal tract.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123870"},"PeriodicalIF":10.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guar gum succinate: Dry synthesis, characterization and application as an efficient adsorbent for removal of safranin-O dye 瓜尔胶琥珀酸盐：干法合成、表征及其在红花素- o染料脱除中的应用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-11 DOI: 10.1016/j.carbpol.2025.123898

Jyoti Tripathi, Sumit Gupta, Bibhuti Bhusan Mishra

{"title":"Guar gum succinate: Dry synthesis, characterization and application as an efficient adsorbent for removal of safranin-O dye","authors":"Jyoti Tripathi, Sumit Gupta, Bibhuti Bhusan Mishra","doi":"10.1016/j.carbpol.2025.123898","DOIUrl":"10.1016/j.carbpol.2025.123898","url":null,"abstract":"<div><div>The development of novel biopolymer derivatives is an emerging approach for remediation of toxic dye contaminated water. During current study guar gum succinate (GGS) was synthesized under dry condition without employing solvents and bases. An equi-proportion mixture of guar gum and succinic anhydride under heat treatment (120 °C, 40 min) resulted in highest degree of substitution (1.05 ± 0.07) for GGS. Further, GGS was characterized and explored as an adsorbent for removal of toxic safranin-O dye from aqueous solutions. The dye adsorption followed pseudo-second-order kinetics, with adsorption mechanism involving boundary layer diffusion followed by intra-particle diffusion into GGS matrix. Fourier transform infrared <strong>(</strong>FT-IR) and Carbon-13 Nuclear Magnetic Resonance (<sup>13</sup>C NMR) spectra revealed strong interactions between aromatic and methyl groups of dye with carbonyl and hydroxyl groups of GGS. Isotherm modelling using Langmuir and Freundlich models confirmed favourable nature of safranin-O adsorption on GGS. Adsorption studies showed maximum monolayer adsorption capacity of 113.64 mg/g, surpassing most reported adsorbents. Furthermore, reusability of GGS was established with over 85 % adsorption efficiency maintained across four cycles, emphasizing its economic viability. Compared to conventional adsorbents, GGS offered an environment friendly, biocompatible, and scalable solution for wastewater treatment, with significant potential in hazardous dye-laden industrial effluent management.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123898"},"PeriodicalIF":10.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication and characterization of novel β-sitosterol-loaded O/W Pickering emulsions stabilized by edible insects protein/chitosan complex coacervates: Retention and stability evaluation 食用昆虫蛋白/壳聚糖络合物稳定的新型β-谷甾醇负载O/W皮德林乳剂的制备和表征：保留性和稳定性评价

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-10 DOI: 10.1016/j.carbpol.2025.123892

Patryk Pokorski , Barbara Strojny - Cieślak , Ewa Domian , Michał Załęcki , Anna Grygier , Michał Pruchniewski , Anna Zakrzewska , Havva Aktaş , Marek Aljewicz , Dominik Kmiecik , Jorge A. Custodio - Mendoza , Piotr Boruszewski , Marcin A. Kurek

{"title":"Fabrication and characterization of novel β-sitosterol-loaded O/W Pickering emulsions stabilized by edible insects protein/chitosan complex coacervates: Retention and stability evaluation","authors":"Patryk Pokorski , Barbara Strojny - Cieślak , Ewa Domian , Michał Załęcki , Anna Grygier , Michał Pruchniewski , Anna Zakrzewska , Havva Aktaş , Marek Aljewicz , Dominik Kmiecik , Jorge A. Custodio - Mendoza , Piotr Boruszewski , Marcin A. Kurek","doi":"10.1016/j.carbpol.2025.123892","DOIUrl":"10.1016/j.carbpol.2025.123892","url":null,"abstract":"<div><div>This study investigates the application of edible insect protein–chitosan (IP/CS) complex coacervates, derived from T. molitor, A. domesticus, and L. migratoria proteins combined with low-, medium-, or high-molecular-weight chitosans, as stabilizers in Pickering emulsions aimed at enhancing emulsion stability and improving the retention of bioactive compounds. The underlying hypothesis posits that IP/CS can function as effective stabilizing systems in Pickering emulsions, providing high overall stability, low susceptibility to destabilization, and efficient encapsulation of lipophilic bioactives. Experimental results showed that IP/CS-based Pickering emulsions achieved high β-sitosterol retention, ranging from 56 % to 91 %. Emulsion stability was primarily attributed to electrostatic interactions and hydrogen bonding within the coacervates. In addition, an effective stabilization mechanism was identified, involving the physical integration of oil droplets into the IP/CS matrix, which contributed to reduced flocculation. Fourier-transform infrared (FTIR) spectroscopy and rheological analysis confirmed the presence of hydrogen bonding and indicated a viscous character of the emulsions, while ζ-potential measurements revealed surface charge properties that further supported emulsion stabilization. These findings demonstrate the role of intermolecular interactions in maintaining the integrity of oil-in-water (O/W) Pickering emulsions stabilized by IP/CS complexes.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123892"},"PeriodicalIF":10.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational design of freeze-crosslinked polysaccharide sponges for efficient non-compressible hemostasis and liver repair 冷冻交联多糖海绵的合理设计，用于有效的非压缩性止血和肝脏修复

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-10 DOI: 10.1016/j.carbpol.2025.123889

Peng Zhou , Weixiao Ding , Hongyan Wang, Xinmeng Li, Jian Cui, Guangjiu Liu, Zhiqi Chen, Chao Tang, Yishan Jiang, Lei Zhang, Dongping Sun

{"title":"Rational design of freeze-crosslinked polysaccharide sponges for efficient non-compressible hemostasis and liver repair","authors":"Peng Zhou , Weixiao Ding , Hongyan Wang, Xinmeng Li, Jian Cui, Guangjiu Liu, Zhiqi Chen, Chao Tang, Yishan Jiang, Lei Zhang, Dongping Sun","doi":"10.1016/j.carbpol.2025.123889","DOIUrl":"10.1016/j.carbpol.2025.123889","url":null,"abstract":"<div><div>Non-compressible hemorrhage is a life-threatening challenge, and shape-memory hemostatic sponges that require no removal are ideal for inaccessible hemorrhage wounds. In this study, we freeze-crosslinked two polysaccharides by tuning their ratios to fully utilize their inherent physiochemical properties. The obtained shape memory sponges exhibit tunable mechanical properties, high absorbent capacities, and compression resilience. The effect of Oxidizing bacteria cellulose (OBC)/Carboxymethyl chitosan (CMCS) ratios on their hemostatic behaviors and liver repair was also systemically studied. In particular, both <em>in vitro</em> coagulation and <em>in vivo</em> hemostasis experiments suggest that the sponges of high OBC/CMCS ratio perform better in terms of compression resilience and hemostasis. The <em>in vitro</em> culture of primary hepatocytes and histological analysis of livers implanted with OBC/CMCS sponges suggest those of high OBC/CMCS ratios are conducive for hepatocytes adhesion and promote liver repair by microvessel formation and cell infiltration with reduced inflammatory effects or foreign body reactions. These easily manufactured hemostatic sponges are suitable for uncontrollable hemorrhage and <em>in situ</em> tissue repair, providing guidance for the rational design of next-generation hemostatic materials.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123889"},"PeriodicalIF":10.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green and efficient electrospinning of ethyl cellulose-based nanofibrous membrane for high-performance and antibacterial air filtration 绿色高效静电纺丝用于高效抗菌空气过滤的乙基纤维素基纳米纤维膜

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-10 DOI: 10.1016/j.carbpol.2025.123893

Ruixin Chen , Qibin Wang , Ruimin Shen , Zeqian Gui , Yuhang Yan , Min Tang , Zhiwei Chen , Zungui Shao , Gaofeng Zheng

{"title":"Green and efficient electrospinning of ethyl cellulose-based nanofibrous membrane for high-performance and antibacterial air filtration","authors":"Ruixin Chen , Qibin Wang , Ruimin Shen , Zeqian Gui , Yuhang Yan , Min Tang , Zhiwei Chen , Zungui Shao , Gaofeng Zheng","doi":"10.1016/j.carbpol.2025.123893","DOIUrl":"10.1016/j.carbpol.2025.123893","url":null,"abstract":"<div><div>The environmentally friendly, economical, and efficient production of high-performance and multifunctional bio-based air filter membranes is crucial for the protection of human health and sustainable development. However, this endeavor is significantly limited by the current gap in material selection and process routes. This study proposes a strategy for advanced functionalization and structural optimization of ethyl cellulose (EC) to effectively develop a high-performance, fully bio-based air filter membrane exhibiting superior antibacterial properties. Through the minimal interference method, konjac glucomannan (KGM) and curcumin (Cur) were blended with EC, which preserved the bimodal fibers to the highest degree. By green electrospinning in one step, the quality factor of EC/KGM/Cur membranes reached 0.101 Pa<sup>−1</sup>, the antibacterial activity against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em> reached >99.6 %, and the productivity of EC/KGM/Cur membranes prepared by using sheath gas was increased by almost 14.73 times. This study offers a sustainable, scalable, and versatile manufacturing approach for air filters and thus a promising solution to the urgent need for environmentally friendly and efficient filter systems.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123893"},"PeriodicalIF":10.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan-based Janus dressing with asymmetric wettability for rapid hemostasis, antibacterial protection, and tissue regeneration in hemorrhagic wound management 壳聚糖基Janus敷料具有非对称润湿性，用于快速止血、抗菌保护和出血性伤口的组织再生

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-10 DOI: 10.1016/j.carbpol.2025.123891

Jiyuan Wang , Haowen Ye , Kaitong He , Weikang Jiang , Wei Lan , Zu’an Zeng , Peiran Lin , Han Wang , Botao Gao , Wenlong Wang , Yadong Tang

{"title":"Chitosan-based Janus dressing with asymmetric wettability for rapid hemostasis, antibacterial protection, and tissue regeneration in hemorrhagic wound management","authors":"Jiyuan Wang , Haowen Ye , Kaitong He , Weikang Jiang , Wei Lan , Zu’an Zeng , Peiran Lin , Han Wang , Botao Gao , Wenlong Wang , Yadong Tang","doi":"10.1016/j.carbpol.2025.123891","DOIUrl":"10.1016/j.carbpol.2025.123891","url":null,"abstract":"<div><div>Multifunctional wound dressings with rapid hemostasis, infection prevention, and tissue regeneration are crucial for treating hemorrhagic wounds. Inspired by the heterogeneous interfacial structure of natural skin, this study developed a chitosan(CS)-based Janus wound dressing with asymmetric wettability. The inner layer consists of methacrylated chitosan (CSMA), incorporating polydopamine-coated silica (PDA-SiO₂) nanoparticles to form a hydrophilic sponge with hemostatic, antioxidant, and pro-angiogenic properties. The outer layer is a thermoplastic polyurethane (TPU) nanofiber membrane integrated with antibacterial agent zeolitic imidazolate framework-8 (ZIF-8), creating a hydrophobic barrier to prevent bacterial invasion. <em>In vitro</em> tests confirmed effective bacterial resistance, while hemostasis assays demonstrated enhanced platelet and red blood cell aggregation, accelerating coagulation. <em>In vivo</em> evaluations using rat liver injury and tail amputation models confirmed superior hemostatic performance, with blood loss reduced by 0.44 g and 0.23 g, respectively, and bleeding time shortened by 41.52 s and 59.47 s compared to Gauze. Furthermore, in full-thickness skin wound models, accelerated healing was achieved through infection prevention, increased collagen deposition (20.82 % higher than Control), and stimulated angiogenesis (3.10 % higher CD31 expression). These findings highlight CM@PS/TZ as a promising Janus dressing integrating antibacterial activity, hemostasis, and pro-angiogenic effects, offering an innovative solution for hemorrhagic wound treatment.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123891"},"PeriodicalIF":10.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhamnogalacturonan-I oligogalacturonide profiling, a tool to elucidate the mode of action of RGI-degrading enzymes and RGI structure 鼠李糖半乳糖醛酸- i寡聚半乳糖醛酸分析，一种阐明RGI降解酶的作用模式和RGI结构的工具

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-10 DOI: 10.1016/j.carbpol.2025.123894

Adrien Lemaire , Catalina Duran-Garzon , Sébastien Rigaud , Pauline Trezel , Olivier Habrylo , Josip Safran , Aline Voxeur , Romain Roulard , Justine Miternique , Jean-Xavier Fontaine , Roland Molinié , Anaïs Guillaume , Loïc Levavasseur , Valérie Lefebvre , Serge Pilard , Jérôme Pelloux , Corinne Pau-Roblot

{"title":"Rhamnogalacturonan-I oligogalacturonide profiling, a tool to elucidate the mode of action of RGI-degrading enzymes and RGI structure","authors":"Adrien Lemaire , Catalina Duran-Garzon , Sébastien Rigaud , Pauline Trezel , Olivier Habrylo , Josip Safran , Aline Voxeur , Romain Roulard , Justine Miternique , Jean-Xavier Fontaine , Roland Molinié , Anaïs Guillaume , Loïc Levavasseur , Valérie Lefebvre , Serge Pilard , Jérôme Pelloux , Corinne Pau-Roblot","doi":"10.1016/j.carbpol.2025.123894","DOIUrl":"10.1016/j.carbpol.2025.123894","url":null,"abstract":"<div><div>Pectins play a major role in the control of plant development and are widely used as hydrocolloids in the food industry. Yet, the fine structure of rhamnogalacturonan-I (RGI) pectic domain is difficult to determine owing to its chemical and structural complexity. In this study, we developed a sensitive analytical method based on the chromatographic separation of oligosaccharides derived from RGI, combined with accurate determination of their sizes and side-chains patterns using high resolution (HR) and tandem (MS/MS) mass spectrometry. This method revealed the structure of RGI from various sources following its hydrolysis by three enzymes, two rhamnogalacturonan hydrolase (RHG and RHG B) and one rhamnogalacturonan lyase (RGL) from <em>A. Aculeatinus,</em> that were heterologously expressed<em>.</em> We first defined the biochemical specificities of the recombinant RGI-ase, using enzymatic assays and NMR analyses, to ascertain their specific activities towards RGI. Finally, employing a specific library of >420 RGI-oligos, built from chromatography and mass spectrometry data, we assessed their mode of action by the analysis of the degradation products. Altogether, our results highlight that this method is of prime importance for analysis of RGI fine structure and to reveal differences in the enzyme's specificities related to peculiar structural motifs.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123894"},"PeriodicalIF":10.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0