Carbohydrate Polymers最新文献_第2页

A chitosan-based composite scaffold as a bioactive strontium-delivery system mediating accelerated bone regeneration 壳聚糖基复合支架作为生物活性锶递送系统加速骨再生

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-02-08 DOI: 10.1016/j.carbpol.2026.125082

Jiyang Zeng , Wei Li , Yawei Li , Zhiming Tu , Hong Ma , Yuliang Dai , Bing Wang , Hongbing Deng

{"title":"A chitosan-based composite scaffold as a bioactive strontium-delivery system mediating accelerated bone regeneration","authors":"Jiyang Zeng , Wei Li , Yawei Li , Zhiming Tu , Hong Ma , Yuliang Dai , Bing Wang , Hongbing Deng","doi":"10.1016/j.carbpol.2026.125082","DOIUrl":"10.1016/j.carbpol.2026.125082","url":null,"abstract":"<div><div>Bone defects caused by trauma, tumor resection, or infection represent significant clinical challenges in orthopaedic practice. Current bone graft materials are often limited by suboptimal osteogenic capacity, donor site morbidity, and insufficient mechanical properties. In this study, we developed a composite bone regeneration scaffold composed of chitosan, silk fibroin fibers, and strontium alginate microspheres (CS/SF@SrAlg) via microfluidic electrospray coupled with fiber fragmentation-recombination techniques. This chitosan-based composite scaffold combines excellent biocompatibility, wide availability, and enhanced mechanical properties. Chemical cross-linking and physical interactions among the components facilitated the formation of a composite system for controlled Sr<sup>2+</sup> release. The scaffold exhibits a hierarchically porous architecture that promotes cellular infiltration, while the silk fibroin fibers improve mechanical integrity and help maintain an optimal microenvironment. In vitro experiments demonstrated that the scaffold significantly enhanced cell proliferation, migration, and osteogenic mineralization, with a cell proliferation rate of more than 120%. In a rat calvarial defect model, the scaffold markedly promoted angiogenesis and osteogenesis, with 81.8% ± 1.48% bone regeneration coverage observed at 8 weeks post-implantation. Mechanistically, RNA transcriptomics revealed that it promoted osteogenesis through modulation of the key PI3K-Akt signaling pathway. Collectively, the CS/SF@SrAlg scaffold represents a promising biomaterial strategy for bone defect repair with substantial potential for clinical application.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125082"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine structural features of polysaccharides and gut microbiota Co-regulate mucin O-glycosylation: Mechanisms and advances 多糖的精细结构特征和肠道菌群共同调节粘蛋白o糖基化：机制和进展

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2025-12-23 DOI: 10.1016/j.carbpol.2025.124859

Tong Zhao, Bo Zhang, Xuansheng Hu, Feng Jia, Qianna Zheng, Ting Lu, Siyuan Tan, Wuying Lang, Nianwu He, Xuhua Liang

{"title":"Fine structural features of polysaccharides and gut microbiota Co-regulate mucin O-glycosylation: Mechanisms and advances","authors":"Tong Zhao, Bo Zhang, Xuansheng Hu, Feng Jia, Qianna Zheng, Ting Lu, Siyuan Tan, Wuying Lang, Nianwu He, Xuhua Liang","doi":"10.1016/j.carbpol.2025.124859","DOIUrl":"10.1016/j.carbpol.2025.124859","url":null,"abstract":"<div><div>Mucin <em>O</em>-glycosylation is a key mechanism maintaining intestinal homeostasis and involving complex crosstalk between mucin glycan structures and microbial metabolism. Polysaccharides, as primary carbon sources for gut microbes, exert structure-dependent effects—through monosaccharide composition, glycosidic linkages, molecular weight, branching patterns, and chemical modifications—on microbial colonization and metabolism. Microbial metabolites, including short-chain fatty acids (SCFAs) and bile acids, further regulate host glycosyltransferase expression, thereby remodeling mucin <em>O</em>-glycans. Meanwhile, bacterial glycosidases (<em>e.g.</em>, fucosidases and sialidases) dynamically remodel Core 1/Core 2 structures and terminal decorations, altering mucus properties and microbial adhesion. This review summarizes how polysaccharide fine structures influence the microbial community composition and mucin <em>O</em>-glycosylation, emphasizing the “SCFAs–G protein-coupled receptor (GPCR) / Histone deacetylase (HDAC)” axis in glycosyltransferase regulation. Analyses of disease models reveal that reduced sulfation/sialylation and core-structure decomplexification are hallmarks of pathological mucin remodeling, with distinct mechanisms across disease contexts. For example, in inflammatory bowel disease (IBD), H₂S-induced disruption of mucin disulfide bonds synergizes with microbial enzymes, whereas in allergic models, SCFA–Treg signaling modulates glycosylation indirectly. Finally, we highlight structural design of polysaccharides, microbial modulation, and enzyme inhibition as potential strategies for therapeutic intervention and propose glycan-targeted prebiotics for precise regulation of mucosal barrier function.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 124859"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146116455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional hyaluronic acid/gelatin hydrogel accelerates the closure and healing of diabetic wounds 功能性透明质酸/明胶水凝胶加速糖尿病伤口的闭合和愈合

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-02-02 DOI: 10.1016/j.carbpol.2026.125045

Qianqian Du , Juan Liu , Chuanfeng Ding , Xiaofei Wang , Liangsheng Li , Qian Feng , Hao Tang , Qian Zhou , Yongsheng Yu

{"title":"Functional hyaluronic acid/gelatin hydrogel accelerates the closure and healing of diabetic wounds","authors":"Qianqian Du , Juan Liu , Chuanfeng Ding , Xiaofei Wang , Liangsheng Li , Qian Feng , Hao Tang , Qian Zhou , Yongsheng Yu","doi":"10.1016/j.carbpol.2026.125045","DOIUrl":"10.1016/j.carbpol.2026.125045","url":null,"abstract":"<div><div>The healing of extensive drug-resistant and skin area diabetic wounds poses significantly greater challenges compared to the recovery of typical wounds. Advanced approaches like tissue engineering and regenerative medicine hold promise, exemplified by hyaluronic acid/gelatin/poly(N-isopropyl acrylamide) (PNIPAm) hydrogel, a temperature-sensitive auto-shrinkage hydrogel with injectable biomimetic mechanical functions. Here, aldehyde-modified hyaluronic acid (HA-CHO) and hydrazide-grafted gelatin (Gel-ADH) are chosen, crosslinked to form a gel by the Schiff base reaction, introduced with PNIPAm to accelerate the wound contraction, and loaded with the pro-angiogenic peptide Ten-2 and the antimicrobial peptides IB-367. Both <em>in vitro</em> and <em>in vivo</em> studies illustrate their effectiveness in accelerating and facilitating the healing process of skin wounds. The hydrogel is able to consistently and successfully suppress the growth of bacteria in the wound, and upregulate the expression of pro-inflammatory factors TGF-β1, α-SMA, and p-Smad2, all of which could aid in the healing process of diabetic wounds. This injectable hydrogel containing antimicrobial peptides and accelerating wound healing mimics the contractile characteristics of myofibroblasts through its biomimetic mechanical mechanism. It can adapt to the dynamic changes of the skin and help repair diabetic wounds that are challenging to treat and may prove to be a good option for regenerative medicine.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125045"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146116458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increases in cell-wall homogalacturonan but decreases in xylogalacturonan accompany transition from dormant to vegetative stages in Chrysolaena obovata rhizophores 卵黄金葵根状茎从休眠阶段向营养阶段过渡时，细胞壁高半乳糖酸增加而木质半乳糖酸减少

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-02-10 DOI: 10.1016/j.carbpol.2026.125086

Marina Câmara Mattos Martins , Fernanda dos Santos Kretzschmar , Nicholas C. Carpita , Adriana Hissae Hayashi , Maria Angela Machado de Carvalho , Breno Leite , Marcia Regina Braga

{"title":"Increases in cell-wall homogalacturonan but decreases in xylogalacturonan accompany transition from dormant to vegetative stages in Chrysolaena obovata rhizophores","authors":"Marina Câmara Mattos Martins , Fernanda dos Santos Kretzschmar , Nicholas C. Carpita , Adriana Hissae Hayashi , Maria Angela Machado de Carvalho , Breno Leite , Marcia Regina Braga","doi":"10.1016/j.carbpol.2026.125086","DOIUrl":"10.1016/j.carbpol.2026.125086","url":null,"abstract":"<div><div>The rhizophores of <em>Chrysolaena obovata</em>, a perennial herb native to the Brazilian Cerrado, are underground storage organs that accumulate fructans and support vegetative propagation. Here, we investigated how the content of fructans and the composition of rhizophore cell wall polysaccharides change across developmental stages and contribute to seasonal adaptation. In addition to the accumulation of fructans in vacuoles (∼26–55% of the dry mass according to the developmental stage), cell-wall polysaccharide composition changed throughout the growth cycle, reflecting structural adjustments linked to developmental and environmental cues. These cell walls have a type I architecture, typical of eudicots. Although total pectin content remained stable, homogalacturonans increased in proportion during the transition from dormant to vegetative stages, whereas amounts of xylogalacturonans decreased. Xylogalacturonans accumulate within the middle lamella during the dormant phase and are potentially associated with flexibility and water retention. Hemicelluloses consisted mainly of low-substituted arabinoxylans and xyloglucan, consistent with a secondary cell wall profile. Xylans decreased slightly in the transition from the dormant to vegetative stage, while xyloglucan proportions increased. Overall, our findings show that seasonal metabolic changes in <em>C. obovata</em> involve dynamic modifications of rhizophore cell wall polysaccharides, which contribute to organ development and drought adaptation throughout the seasonal cycle.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125086"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural determinants of gelation kinetics in rambutan seed starches: Implications for enhanced precision in 3D food printing 红毛丹种子淀粉凝胶动力学的结构决定因素：对提高3D食品打印精度的影响

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-01-31 DOI: 10.1016/j.carbpol.2026.125024

Rui He , Yuxuan Li , Xiaoyu Lin , Bo Xu , Xiang Huo , Weiming Zhang , Tao Yang , Geng Zhong , Fengwei Xie , Ying Chen

{"title":"Structural determinants of gelation kinetics in rambutan seed starches: Implications for enhanced precision in 3D food printing","authors":"Rui He , Yuxuan Li , Xiaoyu Lin , Bo Xu , Xiang Huo , Weiming Zhang , Tao Yang , Geng Zhong , Fengwei Xie , Ying Chen","doi":"10.1016/j.carbpol.2026.125024","DOIUrl":"10.1016/j.carbpol.2026.125024","url":null,"abstract":"<div><div>Precise modulation of starch gelation kinetics is essential for achieving high-fidelity 3D printing of functional foods; however, the narrow processing window of conventional starches remains a primary bottleneck. Since native starch molecular structures are source-dependent and difficult to tailor through modification, the discovery of novel starch sources is pivotal for developing high-performance starch-based inks. This study posits that structurally-diverse starches from underutilized sources offer a fundamental strategy to address this challenge. We characterized three rambutan seed starch varieties (BR-4, BR-5, and BR-7) across multiple structural scales and assessed their 3D food printing performance. The extracted starches featured small, monodisperse spherical granules (7.28–8.26 μm), high molecular weight (9.99 × 10<sup>4</sup>–1.29 × 10<sup>5</sup> kDa), moderate amylose content (21.59–25.70%), rapid room-temperature gelation (2–10 min), and high slowly digestible starch content (69.71–73.45%). All resulting gels satisfied the IDDSI Level 5 (minced & moist) criteria. Remarkably, BR-7, with its higher amylose content and abundant short-chain amylopectin, formed a dense gel network. This unique structural configuration conferred excellent properties including gelation rate (2 min, 11× faster than pea starch), gel strength, printing accuracy (99.14%), and digestion resistance. This study offers insights into the valorization of tropical fruit processing by-products for precision 3D food printing applications.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125024"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-analytical profiling of glycoside hydrolase 13 (GH13) α-amylases on potato starch 马铃薯淀粉中糖苷水解酶13 (GH13) α-淀粉酶的多组分分析

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-02-03 DOI: 10.1016/j.carbpol.2026.125029

Signe Schram Zinck , Valentina N. Perna , Christinne Hedberg Hyldgaard , Tine Hoff , Anne S. Meyer

{"title":"Multi-analytical profiling of glycoside hydrolase 13 (GH13) α-amylases on potato starch","authors":"Signe Schram Zinck , Valentina N. Perna , Christinne Hedberg Hyldgaard , Tine Hoff , Anne S. Meyer","doi":"10.1016/j.carbpol.2026.125029","DOIUrl":"10.1016/j.carbpol.2026.125029","url":null,"abstract":"<div><div>In this study, 20 α-amylases covering several glycoside hydrolase 13 (GH13) subfamilies were characterized with regard to their impact on the molecular properties of gelatinized potato starch. Using a multi-analytical approach, the enzyme-induced changes in the relative amylose content, molecular weight distribution, chain length distribution, and vibrational properties (FTIR) of the starch were assessed. The results showed that structurally and phylogenetically diverse α-amylases produced distinct starch degradation patterns, resulting in different molecular properties of the starch. Principal component analysis (PCA) of the starch molecular weight and chain length distribution data enabled functional classification of the enzymes into six distinct groups, revealing amylopectin-degrading ability as a central performance parameter. While subfamily classification alone turned out to be a less reliable predictor of the α-amylases' starch degradation patterns, protein structural features – particularly active site openness – were found to correlate well with enzymatic substrate preference and proposed mode-of-action. The study highlights the benefits of a multi-analytical framework for differentiating functional nuances of α-amylases beyond conventional classification schemes, potentially providing a basis for rational selection of these for targeted starch modification in the future.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125029"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress decomposition and multimeric assembly evolution of ionotropic alginate hydrogels in large amplitude oscillatory shear flow 海藻酸盐离子型水凝胶在大振幅振荡剪切流中的应力分解和多聚体组装演化

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-01-31 DOI: 10.1016/j.carbpol.2026.125043

Changyao Liu, Wei Yu, Sijun Liu

{"title":"Stress decomposition and multimeric assembly evolution of ionotropic alginate hydrogels in large amplitude oscillatory shear flow","authors":"Changyao Liu, Wei Yu, Sijun Liu","doi":"10.1016/j.carbpol.2026.125043","DOIUrl":"10.1016/j.carbpol.2026.125043","url":null,"abstract":"<div><div>Sodium alginate (SA) hydrogels show complex rheological behaviors, which is related to the existence of multiple junctions, trapped entanglements, and network defects. Understanding the evolution of various structural components of SA networks in large amplitude oscillatory shear (LAOS) flow remains challenging. Herein, we develop a shear cessation method on Lissajous curves and propose a double stretch-exponential (DSE) model for analyzing multiple relaxations of SA networks. The results indicate that the response of SA networks in LAOS flow is able to be decomposed into strain rate-related network defects and the strain-related surviving network. Furthermore, a strain hardening (SH) model is developed to elucidate the strain hardening behavior of surviving network. As a result, the combination of the stress decomposition with the SH model allows us to explore the evolution of various structural components of SA networks with varying association strengths and strain amplitudes. A high association strength produces a low network defect content and a dense surviving network, while an increase in strain amplitude promotes the dissociation of multimeric assembly and the liberation of trapped entanglement. This study provides us with valuable insights to understand the microstructural origin of nonlinear mechanics, which will merit the design of high-performance polysaccharide biomaterials.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125043"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on starch-water interactions by NMR relaxation, thermogravimetric analysis, and their relationship with the viscoelastic properties of rice pasta dough enriched with egg preparations 用核磁共振弛豫、热重分析研究了淀粉-水相互作用及其与蛋制剂大米面食粘弹性的关系

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-01-26 DOI: 10.1016/j.carbpol.2026.125017

Magdalena Witek , Matthew Krzystyniak , Ireneusz Maciejaszek , Joanna Banaś

{"title":"Studies on starch-water interactions by NMR relaxation, thermogravimetric analysis, and their relationship with the viscoelastic properties of rice pasta dough enriched with egg preparations","authors":"Magdalena Witek , Matthew Krzystyniak , Ireneusz Maciejaszek , Joanna Banaś","doi":"10.1016/j.carbpol.2026.125017","DOIUrl":"10.1016/j.carbpol.2026.125017","url":null,"abstract":"<div><div>This study integrates NMR relaxation, thermogravimetric analysis, and stress-relaxation measurements to investigate the role of egg-derived components in rice flour-based gluten-free dough. Principal component analysis (PCA) revealed consistent correlations across the three methods, highlighting protein content as the dominant factor controlling starch-water interactions, water mobility, and viscoelastic behaviour. A critical protein threshold of 7–8% was identified in the studied dough samples, above which proteins markedly modified hydration dynamics and dough structure. Below this level, moisture and egg lipids - particularly from yolk - contributed to enhanced solid-like behaviour by redistributing water through hydrophobic interactions. The complementary nature of the applied methods provided a coherent picture of dough behaviour and identified the relative proportions of protein, fat, and water as key determinants of its rheological properties, marking the first step in rice-flour-based dough formulation.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125017"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutations in PilZ domains of newly discovered bacterial β-(1,3;1,4)-mixed linkage glucan synthases modulate polysaccharide production 新发现的细菌β-(1,3;1,4)-混合连锁葡聚糖合成酶的PilZ结构域突变调节多糖的产生

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-02-07 DOI: 10.1016/j.carbpol.2026.125076

Rebecka Karmakar Saldivar , Shu-Chieh Susie Chang , Sheileza Rajkumari , Rico A. Pratama , Yen-Ting Lin , Mu-Rong Kao , Christina Divne , Chi-Kung Ni , Yves S.Y. Hsieh

{"title":"Mutations in PilZ domains of newly discovered bacterial β-(1,3;1,4)-mixed linkage glucan synthases modulate polysaccharide production","authors":"Rebecka Karmakar Saldivar , Shu-Chieh Susie Chang , Sheileza Rajkumari , Rico A. Pratama , Yen-Ting Lin , Mu-Rong Kao , Christina Divne , Chi-Kung Ni , Yves S.Y. Hsieh","doi":"10.1016/j.carbpol.2026.125076","DOIUrl":"10.1016/j.carbpol.2026.125076","url":null,"abstract":"<div><div>β-(1,3;1,4)-Mixed linkage glucans (MLGs) are linear homopolysaccharides composed of β-1,4-linked glucose oligomers interconnected by β-1,3 linkages. While previous studies have characterised MLG synthases from <em>Romboutsia ilealis</em> and <em>Clostridium ventriculi</em>, the broader diversity of these enzymes remains underexplored. To expand the repertoire of bacterial MLG synthases, nine glycosyltransferase family 2 (GT2) candidates homologous to <em>R. ilealis</em> GT2 were identified and heterologously expressed in an engineered <em>Saccharomyces cerevisiae</em> strain. Two candidates, <em>Clostridium nigeriense</em> GT2 (<em>Cn</em>GT2) and <em>Clostridium cuniculi</em> GT2 (<em>Cc</em>GT2), catalysed the formation of MLG polymers, albeit at reduced levels relative to the reference enzyme. Structural analysis and mutagenesis identified the PilZ domain's D/NxSxxG motif as a critical regulator of synthase activity. These findings provide a structural basis for optimizing bacterial MLG synthases, facilitating the development of efficient microbial production platforms.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125076"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zooming in on methylation of homogalacturonan pectins: How degree and pattern dictate physicochemical properties, bioactivities, and potential applications 聚焦于均半乳糖酸果胶的甲基化：程度和模式如何决定其理化性质、生物活性和潜在应用

IF 12.5 1区化学

Carbohydrate Polymers Pub Date : 2026-05-15 Epub Date: 2026-02-05 DOI: 10.1016/j.carbpol.2026.125044

Guodong Qiu , Xiaogang Dong , Jiahao Yu , Carine Le Bourvellec , Zhuoyan Hu , Jianyong Wu , Dongmei Wu , Xuwei Liu

{"title":"Zooming in on methylation of homogalacturonan pectins: How degree and pattern dictate physicochemical properties, bioactivities, and potential applications","authors":"Guodong Qiu , Xiaogang Dong , Jiahao Yu , Carine Le Bourvellec , Zhuoyan Hu , Jianyong Wu , Dongmei Wu , Xuwei Liu","doi":"10.1016/j.carbpol.2026.125044","DOIUrl":"10.1016/j.carbpol.2026.125044","url":null,"abstract":"<div><div>Homogalacturonan (HG)-type pectin, as the most abundant and widely used pectin, possesses excellent processing properties and health-promoting effects. The degree and pattern of methylation are key structural features of HG-type pectin that influence its functionalities. However, the relationship between these chemical structures and their functional properties has not been fully explored. Therefore, this paper reviews the methylation and demethylation strategies for regulating the degree of methylation (DM) and degree of blockiness (DB) of HG-type pectin, as well as the effects of DM and DB on its physicochemical properties, bioactivities, and potential applications. DM and DB directly influence the molecular conformation, charge distribution, amphiphilic balance, and gelation behavior of pectin by regulating the number and distribution pattern of carboxyl and methyl ester groups along the HG chain. This further governs its interaction intensity and patterns with substances/cells, leading to differentiated efficacy or functional optimization. This work will promote the targeted design and utilization of HG-type pectin based on DM and DB, as well as research on its structure-function relationship.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"380 ","pages":"Article 125044"},"PeriodicalIF":12.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0