Carbohydrate Polymers最新文献_第2页

Oligosaccharides and oligosaccharide-based noble metal nanoparticles: From preparation to biomedical applications 低聚糖和低聚糖基贵金属纳米颗粒：从制备到生物医学应用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-17 DOI: 10.1016/j.carbpol.2025.124083

Yekai Xu , Haoqiang Liu , Jiangxia Xu , Shengyang Zhou , Dilireba Shataer , Liang Wang , Minwei Zhang

{"title":"Oligosaccharides and oligosaccharide-based noble metal nanoparticles: From preparation to biomedical applications","authors":"Yekai Xu , Haoqiang Liu , Jiangxia Xu , Shengyang Zhou , Dilireba Shataer , Liang Wang , Minwei Zhang","doi":"10.1016/j.carbpol.2025.124083","DOIUrl":"10.1016/j.carbpol.2025.124083","url":null,"abstract":"<div><div>Oligosaccharides are increasingly valuable for preparing noble metal (NM) nanoparticles (NPs) due to excellent biocompatibility and abundant reducing functional groups (e.g., hydroxyl, amino, and aldehyde groups). High polarity oligosaccharides can be directly extracted using polar solvents. Cavitation, thermal effects, and free radicals can break down long-chain polysaccharides into shorter chains, yielding mixed oligosaccharides with low molecular weight polysaccharides as by-products. To further depolymerize polysaccharides into oligosaccharides, oxidizing agents (e.g., acids and hydrogen peroxide) are used to degrade the polysaccharides sufficiently to obtain oligosaccharides. Furthermore, enzymatic degradation minimizes structural damage to the products due to its specificity for glycosidic bonds, making it a widely favored method for preparing structurally specific oligosaccharides. More importantly, amino, hydroxyl, and aldehyde groups in oligosaccharides can be involved in reducing highly stable NM NPs. Notably, oligosaccharide-based NM NPs exhibit tumor-specific accumulation capabilities due to their inherent targeting properties, with accumulation levels at tumor sites 2–3 times higher than those of non-targeted particles. Furthermore, their surface functional groups enable efficient conjugation with drugs and immunomodulatory molecules. Additionally, the photothermal conversion efficiency is over 130 % higher than that of traditional photothermal therapeutic agents. They demonstrate significant potential in biomedical applications, including antibacterial therapy, cancer diagnosis, and treatment.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124083"},"PeriodicalIF":10.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface soft patch-mediated supramolecular co-assembly of lysozyme/konjac glucomannan with optimized antimicrobial performance 表面软贴片介导的溶菌酶/魔芋葡甘露聚糖的超分子共组装及其优化抗菌性能

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-16 DOI: 10.1016/j.carbpol.2025.124072

Shanglin Li , Yulun Wu , Yajuan Li , Longjiang Tian , Ting Zhang , Jingbo Liu , Zhiyang Du

{"title":"Surface soft patch-mediated supramolecular co-assembly of lysozyme/konjac glucomannan with optimized antimicrobial performance","authors":"Shanglin Li , Yulun Wu , Yajuan Li , Longjiang Tian , Ting Zhang , Jingbo Liu , Zhiyang Du","doi":"10.1016/j.carbpol.2025.124072","DOIUrl":"10.1016/j.carbpol.2025.124072","url":null,"abstract":"<div><div>Assembly is a natural process where molecules spontaneously form ordered structures with specific functions. In supramolecular co-assembly, interactions between surface soft patches drive structural changes, though this key aspect is often overlooked. In this study, we propose a strategy for supramolecular co-assembly mediated by surface soft patches and apply this approach to optimize the broad-spectrum antibacterial activity of lysozyme (LYS)-konjac glucomannan (KGM) supramolecular co-assembly. Surface charge distribution revealed distinct soft patches on LYS and KGM, with hydrogen bond and hydrophobic interactions between them driving supramolecular co-assembly. Characterized by a densely packed β-sheet structure, the supramolecular co-assembly exhibited excellent rheological properties and significantly enhanced antibacterial activity across a broad spectrum. Specifically, the antibacterial efficacy against <em>Staphylococcus aureus</em> exceeded 99.94 %, and that against <em>Escherichia coli</em> reached over 99.95 % in the Ca<sup>2+</sup>-KGM-LYS coacervate. This research sheds new light on supramolecular co-assembly mechanisms and paves the way for designing broad-spectrum antibacterial materials.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124072"},"PeriodicalIF":10.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-pot biotransformation of cellulose to amylose through multienzyme cascade with nucleoside diphosphate glucose as glycosyl donors 以二磷酸核苷葡萄糖作为糖基供体，通过多酶级联将纤维素转化为直链淀粉

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-16 DOI: 10.1016/j.carbpol.2025.124076

Yuanyuan Zhang , Hongmiao Yuan , Chuanqi Sun , Xixian Xu , Zonglin Li , Jianqiang Lin , Xin Song , Zhimin Li , Hui Lin , Hongge Chen

{"title":"One-pot biotransformation of cellulose to amylose through multienzyme cascade with nucleoside diphosphate glucose as glycosyl donors","authors":"Yuanyuan Zhang , Hongmiao Yuan , Chuanqi Sun , Xixian Xu , Zonglin Li , Jianqiang Lin , Xin Song , Zhimin Li , Hui Lin , Hongge Chen","doi":"10.1016/j.carbpol.2025.124076","DOIUrl":"10.1016/j.carbpol.2025.124076","url":null,"abstract":"<div><div>Artificial starch production from bioreactors is very promising in terms of amylose's broad applications as well as the possibility of addressing food shortage. We previously built an in vitro cellulose-to-starch pathway, synthesizing amylose from non-food cellulose. A challenge of this pathway lies in its low amylose yield due to the fact that only cellobiose in cellulose hydrolysate can be converted into amylose while cellodextrins with a degree of polymerization (DP) ≥ 3 cannot be utilized. Here, we report a new cellulose-to-amylose pathway through a multienzyme cascade where the phosphorolytic product glucose-1-phosphate (G-1-P) from both cellobiose and cellodextrins with DP ≥ 3 is converted to adenosine diphosphate glucose (ADPG) which then serves as a glycosyl donor for amylose synthesis. With introduction of a low-cost ATP regeneration system catalyzed by polyphosphate kinase (PPK), the amylose yield of one-pot biotransformation of cellulose reached 41.2 % at the cost of only 5 mM ATP input, exhibiting a 1.4-fold increase over the previous pathway. The new cellulose-to-amylose pathway has overcome the limitation in previous pathway and provided an efficient and sustainable bioprocess for artificial starch production as well as for utilization of agricultural residues.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124076"},"PeriodicalIF":10.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic impact of starch-tannic acid complex in regulating gut microbiota to produce propionate 淀粉-单宁酸复合物在调节肠道菌群产生丙酸的协同作用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-16 DOI: 10.1016/j.carbpol.2025.124077

Shuting Duan , Yiming Hu , Xiaowei Zhang , Enpeng Li , Cheng Li

{"title":"Synergistic impact of starch-tannic acid complex in regulating gut microbiota to produce propionate","authors":"Shuting Duan , Yiming Hu , Xiaowei Zhang , Enpeng Li , Cheng Li","doi":"10.1016/j.carbpol.2025.124077","DOIUrl":"10.1016/j.carbpol.2025.124077","url":null,"abstract":"<div><div>Starch-tannic acid complex is a type of resistant starch, while the influence of these complexes with different starch chain-length distributions (CLDs) on gut microbiota is unclear. Therefore, starch-tannic acid complexes were prepared from five commercial starches with diverse CLDs, and their influence on the gut microbiota was explored using <em>in vitro</em> fermentation with human fecal microbiota. For the first time, results showed that wheat and corn starch-tannic acid complexes significantly promoted propionate production (<em>p</em> < 0.05) by up to 88 % compared to their retrograded starch counterparts. This was possibly related to the distinct microbiota composition induced by starch-tannic acid complexes, <em>e.g.</em>, the amount of <em>Segatella</em>, <em>Eubacterium_rectale_ATCC_33656</em>, and <em>CAG-352</em> was all significantly decreased (<em>p</em> < 0.05), while their abundances were negatively correlated with propionate production. Notably, starch-tannic acid complexes with a DP range of 12 < DP ≤ 24 were negatively correlated with the amount of <em>Segatella</em>, suggesting that the CLD of complexes is a crucial factor in determining SCFA production <em>via</em> regulating certain bacteria. Collectively, these results highlight the possibility of applying starch-tannic acid complex to target certain gut bacteria and SCFAs production, ultimately improving human health.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124077"},"PeriodicalIF":10.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-efficiency solar seawater evaporator by foam with hierarchically ordered channels based on carboxymethylated regenerated cellulose fibers 基于羧甲基化再生纤维素纤维的分层通道泡沫高效太阳能海水蒸发器

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-15 DOI: 10.1016/j.carbpol.2025.124071

Hailing Shi , Ruihao Ge , Kai Li , Kui Liu , Yixuan Wang , Fengyu Quan , Kewei Zhang , Xing Tian

{"title":"High-efficiency solar seawater evaporator by foam with hierarchically ordered channels based on carboxymethylated regenerated cellulose fibers","authors":"Hailing Shi , Ruihao Ge , Kai Li , Kui Liu , Yixuan Wang , Fengyu Quan , Kewei Zhang , Xing Tian","doi":"10.1016/j.carbpol.2025.124071","DOIUrl":"10.1016/j.carbpol.2025.124071","url":null,"abstract":"<div><div>Solar energy-driven water evaporation technology presents a sustainable method for seawater desalination and wastewater treatment. However, creating a biomass-based interfacial evaporator that achieves high salt resistance and rapid evaporation rates remains a significant challenge. In this study, we prepared a novel foam from carboxymethylated regenerated cellulose fibers, leveraging their natural orientation properties and excellent hydrophilicity. This foam featured hierarchically ordered water transport channels and numerous hydrophilic groups, which enhanced water transport efficiency and decreased the enthalpy of evaporation. The incorporation of polypyrrole gave it effective photothermal conversion properties. The foam demonstrated strong performance in solar-driven water evaporation, achieving an evaporation rate of 3.57 ± 0.04 kg m<sup>−2</sup> h<sup>−1</sup> under simulated sunlight conditions, with an evaporation efficiency of 93.8 ± 1.5 % and a notable ability to resist salt. This research provides a practical solution for sustainable desalination and holds great potential for the future development of desalination evaporators.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 124071"},"PeriodicalIF":10.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Premna microphylla Turcz pectin-modified diosmetin nanoparticles: A galectin-3 targeting strategy for precise colitis intervention 小叶草果果胶修饰的薯蓣皂苷纳米颗粒：半乳糖凝集素-3靶向策略用于精确的结肠炎干预

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-15 DOI: 10.1016/j.carbpol.2025.124067

Yuanyuan Chen , Qian Zhou , Yue Zhou , Jinlan Huang , Renjie Niu , Jian Ming , Mingfu Wang

{"title":"Premna microphylla Turcz pectin-modified diosmetin nanoparticles: A galectin-3 targeting strategy for precise colitis intervention","authors":"Yuanyuan Chen , Qian Zhou , Yue Zhou , Jinlan Huang , Renjie Niu , Jian Ming , Mingfu Wang","doi":"10.1016/j.carbpol.2025.124067","DOIUrl":"10.1016/j.carbpol.2025.124067","url":null,"abstract":"<div><div>Oral delivery of natural antioxidants represents a promising therapeutic strategy for ulcerative colitis (UC), yet their therapeutic efficacy is hindered by instability and poor accumulation at inflamed sites. To address this, we developed Galectin-3 (Gal-3)-targeted nanoparticles (ZDP-NPs) by encapsulating diosmetin within zein complexes modified with a galactose- and rhamnogalacturonan-I (RG-I)-rich pectin (PMTP, Mw: 228.8 kDa, DM: 34.21 %, RG-I content: 43 %) extracted from <em>Premna microphylla</em> Turcz leaves. Surface plasmon resonance (SPR) analysis confirmed a strong binding affinity between PMTP and Gal-3 (KD = 2.83 μM). ZDP-NPs exhibited uniform spherical morphology (~170 nm), high drug loading (87.9 %) and colloidal stability. Under simulated gastrointestinal conditions, ZDP-NPs showed reduced Dio release (45.6 %) compared to PMTP-free nanoparticles (ZD-NPs, 75.6 %). In vitro studies showed that PMTP surface modification significantly enhanced nanoparticle uptake by LPS-induced macrophages via Gal-3-mediated endocytosis. In vivo colitis experiment of mice revealed that ZDP-NPs selectively accumulated in inflamed colons via Gal-3 targeting, prolonged retention, and significantly alleviated colonic damage by reducing oxidative stress, suppressing pro-inflammatory cytokines, and restoring epithelial barrier integrity. Biocompatibility assays confirmed minimal toxicity of ZDP-NPs. This study presented a food-derived pectin-based nanoplatform for precise colitis intervention through Gal-3 targeting, offering a multifaceted strategy to break the inflammation-oxidative stress cycle.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124067"},"PeriodicalIF":10.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of metabolite-enriched carbohydrates from Theobroma biomass via ultrasound-assisted alkaline extraction 超声辅助碱法提取可可生物质中富含代谢物的碳水化合物

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-15 DOI: 10.1016/j.carbpol.2025.124070

Jose Manuel Núñez-Ramírez , Andrea Martelli , Tabitha Halliday , Bradley Thomas , Corinne Wills , Valeria Cannillo , Devis Bellucci , Carlos Carranza , Paola A. García-Rincón , Piergiorgio Gentile , Joel Girón-Hernández

{"title":"Valorization of metabolite-enriched carbohydrates from Theobroma biomass via ultrasound-assisted alkaline extraction","authors":"Jose Manuel Núñez-Ramírez , Andrea Martelli , Tabitha Halliday , Bradley Thomas , Corinne Wills , Valeria Cannillo , Devis Bellucci , Carlos Carranza , Paola A. García-Rincón , Piergiorgio Gentile , Joel Girón-Hernández","doi":"10.1016/j.carbpol.2025.124070","DOIUrl":"10.1016/j.carbpol.2025.124070","url":null,"abstract":"<div><div>Polysaccharides, widely used in food, pharmaceutical and industrial sectors, are abundant in <em>Theobroma</em> species pod husk waste (<em>T. cacao</em>, <em>T. grandiflorum</em> and <em>T. bicolor</em>). This study examined alkaline extraction using NaOH and KOH with ultrasound assistance to assess the physicochemical and functional properties of the resulting polysaccharides and non-polysaccharides components. The extraction yields of these components varied, with <em>T. cacao</em>-NaOH yielding the highest recovery (38.0 %), followed by <em>T. bicolor</em>-NaOH (14.7 %) and <em>T. grandiflorum</em>-NaOH (8.0 %). NaOH-extracted materials exhibited galacturonic acid content up to 40 %, molecular weight of ∼783 kDa and esterification degree of 38.1 %, while KOH-based extraction retained higher levels of rhamnose (18.0 %) and xylose (28.3 %). Materials extracted via KOH preserved metabolites like proanthocyanidins (1.58 mg catechin equivalent/g in <em>T. grandiflorum</em>), whereas NaOH extraction resulted in greater antioxidant activity, particularly in <em>T. cacao</em> with 127.8 mmol Fe<sup>2+</sup>/g and 3.26 M Trolox equivalent/g in FRAP and ORAC assays. Rheology showed that polysaccharides extracted from <em>T. cacao</em> and <em>T. bicolor</em> exhibited highest viscosity and gel strength (G′ > G″), while those from <em>T. grandiflorum</em> had lower viscosity. Thermal analyses confirmed that NaOH extractions produced stable, gel-forming polysaccharides associated with pectic components. These findings highlight <em>Theobroma</em> pod husks as a valuable waste source for revalorization.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124070"},"PeriodicalIF":10.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heparin-loaded silk fibroin microparticles/bacterial nanocellulose (Hep@SFMPs/BNC) conduits for application as small-caliber artificial blood vessels 载肝素丝素微粒/细菌纳米纤维素（Hep@SFMPs/BNC）用于小口径人工血管的导管

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-15 DOI: 10.1016/j.carbpol.2025.124066

Geli Li , Luhan Bao , Lin Chen , Xingping Zhou , Feng F. Hong

{"title":"Heparin-loaded silk fibroin microparticles/bacterial nanocellulose (Hep@SFMPs/BNC) conduits for application as small-caliber artificial blood vessels","authors":"Geli Li , Luhan Bao , Lin Chen , Xingping Zhou , Feng F. Hong","doi":"10.1016/j.carbpol.2025.124066","DOIUrl":"10.1016/j.carbpol.2025.124066","url":null,"abstract":"<div><div>Small-caliber artificial blood vessels are highly demanded and face challenges, including thrombosis and intimal hyperplasia. The excellent properties of bacterial nanocellulose (BNC) make it an excellent material for preparing artificial blood vessels. Heparin (Hep)-loaded silk fibroin microparticles (SFMPs) were synthesized <em>in situ</em> within the conduit wall <em>via</em> liquid pressure injection and phase separation, aiming to improve BNC's anticoagulant properties. SFMPs served as a carrier for Hep, enabling its sustained slow release from the BNC conduits, and the Hep@SFMPs/BNC promoted the adhesion and proliferation of vascular cells. <em>In vitro</em> blood experiments and gene expression analysis of HUVECs showed that the composite conduits had a decreased coagulation rate, fewer platelets adsorbed, and lower levels of inflammation compared with BNC conduits. After 5 months of abdominal aortic replacement in rats, an <em>in situ</em> formation of a continuous endothelial layer was observed, covering 90 % of the surface with endothelial cells. The proposed preparation method is simple, efficient, and requires fewer reagents, with the added benefit of eliminating the need for crosslinking agents. The Hep@SFMPs/BNC conduit has the characteristics of anticoagulant and endothelial growth-promoting properties, with strong evidence from animal experiments supporting the potential of Hep@SFMPs/BNC conduits as small-caliber artificial blood vessels.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124066"},"PeriodicalIF":10.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulose-based aerogels modulate fragrance adsorption and controlled release by carbonization/in-situ aromatization 纤维素基气凝胶通过炭化/原位芳构化调节香味吸附和控释

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-14 DOI: 10.1016/j.carbpol.2025.124061

Tingyi Zhao , Qidong Zhang , Ke Zhang , Qingzhao Shi , Menglan Xiao , Mingqin Zhao , Miao Lai , Bing Cui

{"title":"Cellulose-based aerogels modulate fragrance adsorption and controlled release by carbonization/in-situ aromatization","authors":"Tingyi Zhao , Qidong Zhang , Ke Zhang , Qingzhao Shi , Menglan Xiao , Mingqin Zhao , Miao Lai , Bing Cui","doi":"10.1016/j.carbpol.2025.124061","DOIUrl":"10.1016/j.carbpol.2025.124061","url":null,"abstract":"<div><div>Fragrances are indispensable additives in consumer products including foods, cosmetics, and tobacco products. However, their inherent instability leads to rapid quality degradation and performance loss, driving the urgent need for controlled-release systems to stabilize fragrance performance. In this work, cellulose nanofibers (CNF) were used to prepare CNF aerogel-like gels (CA) and carbonized CNF aerogels (C-CA) through freeze-drying and high-temperature carbonization. Two model fragrance molecules such as <span>d</span>-limonene (DL) and methyl dihydrojasmonate (MD) were loaded into the aerogels and aerogel-like gels using vapor-phase adsorption and in-situ method. The study systematically evaluated how material types, loading methods, fragrance properties influence adsorption and controlled-release performance. The results demonstrate that the unique porous structure of the materials and the formation of hydrogen bonds contribute to the adsorption and controlled release of fragrance molecules. The fragrance-loaded aerogels and aerogel-like gels achieved tunable loading capacities ranging from 10.04 to 578.09 mg/g. Temperature-controlled experiments demonstrated adjustable fragrance release percentages, enabling the selection of tailored aerogel materials for specific application environments in controlled-release systems. These findings advance the simple design of CNF-based aerogels and aerogel-like gels delivery systems for precise fragrance control.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124061"},"PeriodicalIF":10.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A robust method for quantitative detection of β-1,3-glucan using Limulus polyphemus factor Gα fragment 用鲎因子Gα片段定量检测β-1,3-葡聚糖的可靠方法

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-14 DOI: 10.1016/j.carbpol.2025.124060

Andong Zhou , Yitong Li , Yuanning Wang , Yibing Wang , Zhonghui Qiao , Kevin H. Mayo , Zihan Zhao , Lin Sun

{"title":"A robust method for quantitative detection of β-1,3-glucan using Limulus polyphemus factor Gα fragment","authors":"Andong Zhou , Yitong Li , Yuanning Wang , Yibing Wang , Zhonghui Qiao , Kevin H. Mayo , Zihan Zhao , Lin Sun","doi":"10.1016/j.carbpol.2025.124060","DOIUrl":"10.1016/j.carbpol.2025.124060","url":null,"abstract":"<div><div>β-1,3-glucan is an important glycan in the cell wall of most fungi and plants. Accurate detection of β-1,3-glucans is essential for their use in the food and pharmaceutical industries. Here, we developed a robust method to detect β-1,3-glucan using <em>Limulus polyphemus</em> factor Gα (LFGα) fragments. Five β-1,3-glucans with various degrees of branching were extracted from various edible mushrooms and structurally characterized. Their binding affinities to truncated LFGα variants (Q + D1 + D2, D1 + D2, D1, D2) were evaluated using the ELISA assay. LFGα fragment Q + D1 + D2 exhibited the highest affinity toward all β-1,3-glucans regardless of the degree of branching, whereas fragments D1 + D2, D1, and D2 had reduced affinities as the degree of branching increased. AlphaFold-predicted models and molecular docking supported these findings. The branching-insensitive binding with Q + D1 + D2 enabled us to accurately quantify the amount of β-1,3-glucan using a Q + D1 + D2-based ELISA assay. A standard curve was established using commercial curdlan, and quantitative detection of five mushroom β-1,3-glucans was in agreement with their actual glucan content. Our results demonstrate that the Q + D1 + D2-based ELISA assay can be developed as an efficient method for quantitative detection of β-1,3-glucans.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 124060"},"PeriodicalIF":10.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0