Carbohydrate Polymers最新文献_第3页

Molecular insights into the mechanism between the structural and mechanical properties of glycol chitosan methacrylate hydrogels 乙二醇壳聚糖甲基丙烯酸酯水凝胶结构与力学性能机理的分子研究

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-10 DOI: 10.1016/j.carbpol.2025.123872

Kai-Hsiang Chang , Chia-Hung Wu , Zhen-Jie Gao , Yu-Chia Su , Ken-Tsung Wong , Yi-Cheun Yeh , Jiashing Yu , Chia-Ching Chou

{"title":"Molecular insights into the mechanism between the structural and mechanical properties of glycol chitosan methacrylate hydrogels","authors":"Kai-Hsiang Chang , Chia-Hung Wu , Zhen-Jie Gao , Yu-Chia Su , Ken-Tsung Wong , Yi-Cheun Yeh , Jiashing Yu , Chia-Ching Chou","doi":"10.1016/j.carbpol.2025.123872","DOIUrl":"10.1016/j.carbpol.2025.123872","url":null,"abstract":"<div><div>Glycol chitosan (GC), a derivative of chitosan with ethylene glycol branches, is favored in pharmaceuticals due to its nontoxicity and versatility. When modified with methacrylate, GC forms a photocrosslinking hydrogel, glycol chitosan methacrylate (GCMA), which is highly biocompatible and hydrophilic, making it suitable for drug delivery and tissue engineering applications. However, studies on its fundamental properties are limited. This research uses molecular dynamics simulations and experiments to explore how methacrylation affects GC's structural, mechanical, and molecular characteristics. Results show that methacrylation increases Young's modulus, making the material more brittle with shear-thinning and self-healing properties, consistent with an observed decrease in hydrogen bonding. Simulations reveal that reduced hydrogen bonds shorten the polymer's end-to-end distance, causing it to curl and enhancing its mechanical strength. Furthermore, the hydrophobic nature of the methacrylate group is indicated by reduced hydrogen bonds between molecular chains and water. This study deepens the understanding of GC and GCMA, highlighting the importance of hydrogen bonding, network density, and chemical interactions in determining the material's properties.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123872"},"PeriodicalIF":10.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gelation of κ-carrageenan in water-in-water emulsions κ-卡拉胶在水包水乳剂中的凝胶化

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-09 DOI: 10.1016/j.carbpol.2025.123879

Navya Mary Jose, Taco Nicolai, Frédéric Renou, Lazhar Benyahia

{"title":"Gelation of κ-carrageenan in water-in-water emulsions","authors":"Navya Mary Jose, Taco Nicolai, Frédéric Renou, Lazhar Benyahia","doi":"10.1016/j.carbpol.2025.123879","DOIUrl":"10.1016/j.carbpol.2025.123879","url":null,"abstract":"<div><div>The gelation behaviour of κ-carrageenan (κ-car) in the presence of KCl was examined in binary mixtures with dextran (Dex) or polyethylene oxide (PEO), as well as in water-in-water (W/W) emulsions formed by these polymers. The results show that the presence of Dex or PEO has only a weak effect on κ-car gelation. However, it affects the microstructure rendering the κ-car network more homogenous with increasing viscosity of the DEX or PEO solutions. In the W/W emulsions, κ-car preferentially partitioned to the Dex-rich phase. Rheological measurements showed that the storage modulus (G') increased with the volume fraction of the PEO phase (φ) as long as the Dex phase was continuous. The transition of the DEX phase from continuous to dispersed at φ ≈ 70 % resulted in a sharp reduction of the gel stiffness. Weak gels were nevertheless formed up to φ ≈ 80 % due to aggregation of gelled Dex droplets into a system spanning network. For φ > 90 %, G' increased again sharply as an increasing fraction of the κ-car partitioned to the PEO phase. This work highlights that the rheology of W/W emulsions can be tuned by adding κ-car.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123879"},"PeriodicalIF":10.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moisture-mediated freeze-thaw degradation in bamboo from cellulose hydration to macroscale fracture 水分介导的竹材冻融降解从纤维素水化到宏观断裂

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-09 DOI: 10.1016/j.carbpol.2025.123884

Jian Gan , Yahui Zhang , Qiuqin Lin , Wenji Yu

{"title":"Moisture-mediated freeze-thaw degradation in bamboo from cellulose hydration to macroscale fracture","authors":"Jian Gan , Yahui Zhang , Qiuqin Lin , Wenji Yu","doi":"10.1016/j.carbpol.2025.123884","DOIUrl":"10.1016/j.carbpol.2025.123884","url":null,"abstract":"<div><div>Bamboo, a sustainable material with an excellent strength-to-weight ratio, faces durability challenges in cold climates due to freeze-thaw damage induced by moisture-phase transitions. This study aims to characterize moisture-dependent structural and mechanical evolutions in bamboo fibers across air-dried (Dry<img>B), fiber saturation point (FSP-B), and water-saturated (WS-B) states under ultra-low temperatures. Integrated SEM, SAXS, and mechanical analyses reveal that free water in WS-B generates interfacial stresses via ice crystal expansion, causing macroscale cracks along vascular bundles, while nanoconfined ice crystallization of bound water in FSP-B at −45 °C increases microfibril porosity by 15 %. Cyclic freeze-thaw treatments induce hemicellulose hydrolysis and microfibril disorientation, reducing crystallinity from 85.6 % to 66.8 % and tensile strength by 51 %. Below the FSP, cryogenic strengthening occurs with a 4 % bending strength increase per 5 % moisture gain due to ice-reinforced lumens, whereas post-FSP saturation accelerates damage, with 30-cycle strength retention of 86.5 % (FSP-B) versus 83.5 % (WS-B). Identifying bound water as a nanoscale fibril regulator and free water as a macroscopic fracture initiator, this work suggests moisture control below the FSP to mitigate freeze-thaw damage and leverage ice-mediated reinforcement for frost-resistant bamboo composites and ice-templated bio composites in cold-climate applications, providing a framework for optimizing bio-based material durability in cryogenic environments.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123884"},"PeriodicalIF":10.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complexation-driven 3D printable whey protein-lotus root composite gels for dysphagia foods 复合驱动的3D打印乳清蛋白藕复合凝胶，用于吞咽困难的食物

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-09 DOI: 10.1016/j.carbpol.2025.123864

Jinfeng Wang , Xueyu Jiang , Houze Gan , Siyu Li , Kaidi Peng , Ying Sun , Ming Ma , Yang Yi

{"title":"Complexation-driven 3D printable whey protein-lotus root composite gels for dysphagia foods","authors":"Jinfeng Wang , Xueyu Jiang , Houze Gan , Siyu Li , Kaidi Peng , Ying Sun , Ming Ma , Yang Yi","doi":"10.1016/j.carbpol.2025.123864","DOIUrl":"10.1016/j.carbpol.2025.123864","url":null,"abstract":"<div><div>The development of customizable nutritional foods suitable for individuals with dysphagia remains a significant challenge. This study aimed to design a 3D-printed gel integration lotus root whole powder (WL) and whey protein (WP), tailored to meet the nutritional and textural needs of dysphagia people. Results showed that WP significantly improved the printability of lotus root gel (WLG). The 20 % WP formulation achieved optimal precision, with a base area of 407.77 ± 0.508 mm<sup>2</sup> and a height of 26.04 ± 0.031 mm, representing deviations of only 7.77 mm<sup>2</sup> and 1.04 mm from the designed model dimensions. Multi-scale characterization elucidated the printing mechanism, where WP improved the gel elasticity by reducing the starch molecular cross-linking and forming a microporous network that effectively enhanced moisture retention. Additionally, WP also induced α-helical to β-sheet transitions, strengthening the hydrogen bonding. Hydrophobic interactions promoted protein aggregation, while disulfide bonds facilitated covalent cross-linking, collectively forming a reinforced protein-starch matrix through multi-bond complexation. IDDSI testing confirmed the suitability of the lotus root whole powder/whey protein gel (WP-WLG) for dysphagia management. Furthermore, nutrient-fortified formulations demonstrated printing accuracy exceeding 93 %. The findings elucidate the gelation mechanism of WL-WP complex formation, offering a strategy for developing multi-nutrient, easy-to-swallow foods and a promising approach to personalized dysphagia nutrition.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123864"},"PeriodicalIF":10.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal-polyphenol network-hardened coconut protein-carboxymethyl cellulose complex coacervates: Fabrication, characterization, environmental stability, and release kinetics 金属-多酚网络硬化椰子蛋白-羧甲基纤维素复合凝聚物：制造、表征、环境稳定性和释放动力学

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-09 DOI: 10.1016/j.carbpol.2025.123880

Maye Li , Sha Luo , Chunmei Cheng , Xun Liang , Jing Xu , Zengwang Guo , Zhongjiang Wang , Lechuan Wang , Zhaoxian Huang , Lianzhou Jiang

{"title":"Metal-polyphenol network-hardened coconut protein-carboxymethyl cellulose complex coacervates: Fabrication, characterization, environmental stability, and release kinetics","authors":"Maye Li , Sha Luo , Chunmei Cheng , Xun Liang , Jing Xu , Zengwang Guo , Zhongjiang Wang , Lechuan Wang , Zhaoxian Huang , Lianzhou Jiang","doi":"10.1016/j.carbpol.2025.123880","DOIUrl":"10.1016/j.carbpol.2025.123880","url":null,"abstract":"<div><div>Metal-polyphenol networks (MPNs), as a novel surface structure modifier, whether it can have a hardening effect on complex coacervate microcapsules is still unknown. In this work, piperine (PIP)-loaded coconut protein (CCP)-carboxymethyl cellulose (CMC) complex coacervates (CP) microcapsules were hardened using tannic acid-Fe<sup>3+</sup> networks (TA-Fe<sup>3+</sup>) to improve the stability of microcapsules. The results showed that the optimal coacervation conditions for CCP-CMC were at pH = 3.2 and a CCP: CMC ratio of 2:1 (<em>w</em>/w). TA-Fe<sup>3+</sup> formed a network-decorated coating with a supramolecular structure on the CP surface. TA:Fe<sup>3+</sup> ratios significantly affected the structural cohesion of TA-Fe<sup>3+</sup>-hardened piperine-loaded CCP-CMC complex coacervates (TF) and thus the hardening effect. At low Fe<sup>3+</sup> levels, TF formed a dense and uniform structure, and at high Fe<sup>3+</sup> levels, the TF structure became looser. Besides, 2TA-1Fe<sup>3+</sup>-CP exhibited excellent encapsulation efficiency, desirable stability, and favorable antioxidant ability. After long-term storage, light irradiation, and pH treatment, the retention of PIP in 2TA-1Fe<sup>3+</sup>-CP was increased by 119 %, 133 %, and 111 % compared to CP, respectively. Moreover, the release kinetics results proved that TA-Fe<sup>3+</sup> delayed the release of CP in the food simulants. This study provided some valuable information for exploring a novel hardening microcapsule approach based on the complex coacervation.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123880"},"PeriodicalIF":10.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on chitosan-based sutures: a review 壳聚糖基缝合线的研究进展

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-09 DOI: 10.1016/j.carbpol.2025.123868

Haowen Xu , Huibo Ma , Gang Guo , Cuiying Wei , Xiangyan Zhang , Mingjin Guo , Yongxin Li

{"title":"Research progress on chitosan-based sutures: a review","authors":"Haowen Xu , Huibo Ma , Gang Guo , Cuiying Wei , Xiangyan Zhang , Mingjin Guo , Yongxin Li","doi":"10.1016/j.carbpol.2025.123868","DOIUrl":"10.1016/j.carbpol.2025.123868","url":null,"abstract":"<div><div>Effective wound closure facilitates optimal tissue healing, and sutures are one of the predominant medical textiles utilized for wound closure. Chitosan, a biopolymer derived from chitin, possesses distinctive hemostatic properties, antimicrobial and anti-inflammatory activity, biocompatibility, and biodegradability, making it a prevalent choice for wound closure applications. This review provides a comprehensive summary of the current state of chitosan's application in medical sutures, focusing on recent advancements in the fabrication of chitosan-based sutures through various techniques, including wet spinning, melt spinning, electrospinning, interfacial polyelectrolyte complexes, and coating processes. Furthermore, the challenges and future trends in functionality and preparation technology of chitosan-based sutures were discussed. In conclusion, ongoing advancements in scientific research and technology are anticipated to address current limitations, positioning chitosan-based sutures as an optimal option for surgical applications and enhancing therapeutic outcomes.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123868"},"PeriodicalIF":10.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-pot tailored synthesis of highly monodisperse β-1,3-glucan from sucrose driven by an in vitro self-assembled dual-enzyme system via spy chemistry 在体外自组装双酶系统的驱动下，通过间谍化学一锅合成高度单分散的β-1,3-葡聚糖

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-09 DOI: 10.1016/j.carbpol.2025.123878

Guotao Mao , Ming Song , Jin Yu , Junhan Lin , Zhanhe Wang , Zengping Su , Hui Xie , Hongsen Zhang , Hongge Chen , Andong Song

{"title":"One-pot tailored synthesis of highly monodisperse β-1,3-glucan from sucrose driven by an in vitro self-assembled dual-enzyme system via spy chemistry","authors":"Guotao Mao , Ming Song , Jin Yu , Junhan Lin , Zhanhe Wang , Zengping Su , Hui Xie , Hongsen Zhang , Hongge Chen , Andong Song","doi":"10.1016/j.carbpol.2025.123878","DOIUrl":"10.1016/j.carbpol.2025.123878","url":null,"abstract":"<div><div>β-1,3-Glucan exhibits a broad spectrum of biological activities. However, its broader application has been constrained by current production methods that are uneconomical, inefficient, and environmentally unfriendly. Here, we developed an efficient bottom-up synthesis system for β-1,3-glucan employing thermostable sucrose phosphorylase from <em>Bifidobacterium adolescentis</em> and β-1,3-glucan phosphorylase from <em>Thermosipho africanus</em> using sucrose as a substrate and generating glucose-1-phosphate as a key intermediate. The optimized free enzyme system achieved a 93.1 % conversion of 150 mM sucrose to β-1,3-glucan in 3 h. Furthermore, a self-assembled dual-enzyme system constructed using genetically fused enzymes that self-assemble <em>via</em> SpyCatcher/SpyTag demonstrated a 97.4 % conversion efficiency from 500 mM sucrose in 6 h—a 4-fold enhancement over the free dual-enzyme system due to the direct channeling of 68.5 % of glucose-1-phosphate. This approach enabled the synthesis of β-1,3-glucan with a tunable average degree of polymerization (DP<sub>n</sub>) from 31 to 13 by adjusting the concentrations of glucose primer from 1 to 150 mM. At 1 mM glucose, insoluble β-1,3-glucan (DP<sub>n</sub> = 31) with excellent monodispersity (dispersity index = 1.01) was synthesized at a productivity of 13.2 g/L/h. Overall, this study provides an economically viable and environmentally sustainable strategy for the industrial-scale production of β-1,3-glucan from renewable sucrose, facilitating its broader application.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123878"},"PeriodicalIF":10.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of starch structure, functional properties, and the underlying molecular mechanism in SeaRice86 (SR86) SeaRice86 （SR86）淀粉结构、功能特性及其分子机制分析

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-09 DOI: 10.1016/j.carbpol.2025.123881

Yongxiang Huang, Xiao Yang, Yilan Cui, Mangu Hu, Yunliang Rong, Yu Ling, Rongchao Yang, Yueqin Zhang

{"title":"Analysis of starch structure, functional properties, and the underlying molecular mechanism in SeaRice86 (SR86)","authors":"Yongxiang Huang, Xiao Yang, Yilan Cui, Mangu Hu, Yunliang Rong, Yu Ling, Rongchao Yang, Yueqin Zhang","doi":"10.1016/j.carbpol.2025.123881","DOIUrl":"10.1016/j.carbpol.2025.123881","url":null,"abstract":"<div><div>Starch, the principal carbohydrate in rice, governs functional attributes critical to food processing and nutritional value. This study compares structural, functional, and molecular traits of starch from salt-tolerant SeaRice86 (SR86) and conventional Huanghuazhan (HHZ). Structural analysis revealed SR86 starch exhibits a distinct A-type crystal structure with higher crystallinity at 12 DAF but lower values at 24 DAF. FTIR spectroscopy showed reduced short-range molecular ordering in SR86 starch. Functional assessments demonstrated that SR86 possesses elevated breakdown viscosity and reduced final viscosity, correlating with its lower amylose content and diminished retrogradation tendency. Transcriptomic profiling identified key molecular drivers underlying their starch properties divergences. SR86 exhibited suppressed <em>GBSSI</em> expression, consistent with its lower amylose. Temporal shifts in soluble starch synthase (<em>SS</em>) activity were observed, with peak <em>SS</em> expression occurring later in SR86. Granule size variations corresponded with differential <em>SSI</em> expression, while altered amylopectin chain distribution (DP 25–36) inversely correlated with starch branching enzyme (<em>SBE</em>) activity. Coordinated regulation of isoamylase (<em>ISA1–3</em>) and pullulanase (<em>PUL</em>) genes further contributed to the unique starch architecture. These findings provide a foundation for leveraging SR86 starch in functional food formulations and highlight its potential as a genetic resource for improving starch quality under saline-alkaline cultivation.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123881"},"PeriodicalIF":10.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan and its derivatives as nanotheranostics in multiple diseases management: a clinical perspective 壳聚糖及其衍生物纳米治疗在多种疾病治疗中的临床应用

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-07 DOI: 10.1016/j.carbpol.2025.123852

Komal Rani , Ankit Kumar Malik , Aseem Setia , Nandini Vinodrao Randhave , Nidhi Verma , Vikas Kumar , Vaishali , Kalim Deshmukh , Madaswamy S. Muthu

{"title":"Chitosan and its derivatives as nanotheranostics in multiple diseases management: a clinical perspective","authors":"Komal Rani , Ankit Kumar Malik , Aseem Setia , Nandini Vinodrao Randhave , Nidhi Verma , Vikas Kumar , Vaishali , Kalim Deshmukh , Madaswamy S. Muthu","doi":"10.1016/j.carbpol.2025.123852","DOIUrl":"10.1016/j.carbpol.2025.123852","url":null,"abstract":"<div><div>Chitosan, a biopolymer derived from chitin, has garnered substantial scrutiny in recent times, attributable to its versatile properties, including biodegradability, biocompatibility, and non-toxicity. These characteristics make it an ideal candidate for various medical applications, particularly in the field of nanomedicine. This review explores the emerging role of chitosan and its derivatives in nanotheranostics, which combines therapeutic and diagnostic modalities for the treatment of multiple diseases. The prospect of chitosan-based nanoparticles in the delivery of drugs, vector delivery, imaging, and disease monitoring has been extensively explored. The modification of chitosan with various functional groups, such as chitosan oligosaccharides, thiolated chitosan, carboxymethyl chitosan, glycol chitosan and other derivatives, enhances its pharmacokinetic properties, targeting capabilities, and therapeutic efficacy. Chitosan-based nanocarriers have shown potential in treating various conditions, including cancer, inflammation, infectious diseases, and neurodegenerative disorders, by improving the solubility of drugs, stability, and controlled release. Additionally, chitosan has a theranostic role in imaging approaches such as optical imaging, ultrasound, and photoacoustic imaging, facilitating early diagnosis and monitoring of therapeutic responses. This review also addresses the disputes and future perceptions for the development of chitosan-based nanotheranostics, including issues related to scalability, regulatory approval, and long-term safety.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123852"},"PeriodicalIF":10.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic synthesis and functional characterization of site-specific S/O-linked N-glycopeptides: Elucidating the impact of glycosidic linkage patterns on bioactivity 位点特异性S/ o连接n -糖肽的酶合成和功能表征：阐明糖苷连接模式对生物活性的影响

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-06-06 DOI: 10.1016/j.carbpol.2025.123871

Xiaodan Zhai , Chengyuan Zhang , Jiageng Li , Meng Hao , Jiaqi Lian , Jia Liu , Tao Hu , Wanyi Guan , Zhigang Wu

{"title":"Enzymatic synthesis and functional characterization of site-specific S/O-linked N-glycopeptides: Elucidating the impact of glycosidic linkage patterns on bioactivity","authors":"Xiaodan Zhai , Chengyuan Zhang , Jiageng Li , Meng Hao , Jiaqi Lian , Jia Liu , Tao Hu , Wanyi Guan , Zhigang Wu","doi":"10.1016/j.carbpol.2025.123871","DOIUrl":"10.1016/j.carbpol.2025.123871","url":null,"abstract":"<div><div>Glycoengineering of therapeutic peptides/proteins has been hindered by the fact that no cost-effective and scalable strategy is currently available to install a β-<em>N</em>-acetylglucosamine (GlcNAc) residue to a pre-determined Asn site in peptides/proteins, to be further glycosylated by <em>N</em>-glycan. In this study, we developed a two-step enzymatic method to prepare <em>N</em>-glycan-modified peptides with natural β-<em>S</em>/<em>O</em>-glycosylation linkage by sequentially catalyzed by the <em>S</em>/<em>O</em>-HexNAc-transferase from <em>Streptomyces venezuelae</em> ATCC 15439 (SvGT) and the N180H mutant of endoglycosidase from <em>Coprinopsis cinerea</em> (EndoCC<sup>N180H</sup>). To unravel how glycosylation linkage patterns and glycosylation extent impact the bioactivity of the glycopeptides, cell-based receptor activation potency and <em>in vivo</em> hypoglycemic activity were assayed for β-<em>S</em>/<em>O</em>/<em>N</em>-glycosylated glucagon-like peptide-1 (GLP-1) analogues. The comparable bioactivity exhibited by the <em>S</em>-glycosylated and <em>N</em>-glycosylated products presented <em>S</em>-glycosylation as an efficient and effective strategy for future glycoengineering of peptide/protein pharmaceuticals.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 123871"},"PeriodicalIF":10.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0