Carbohydrate Polymers最新文献_第3页

Polysaccharide-based green flocculants: A systematic and comparative study of their coagulation-flocculation efficiency

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-22 DOI: 10.1016/j.carbpol.2025.123527

Simon Leonhartsberger , Pierre Carmona , Bernhard Seidl , Karl-Juergen Mann , Martin Kozich , Irina Sulaeva , Christian Stanetty , Marko D. Mihovilovic

{"title":"Polysaccharide-based green flocculants: A systematic and comparative study of their coagulation-flocculation efficiency","authors":"Simon Leonhartsberger , Pierre Carmona , Bernhard Seidl , Karl-Juergen Mann , Martin Kozich , Irina Sulaeva , Christian Stanetty , Marko D. Mihovilovic","doi":"10.1016/j.carbpol.2025.123527","DOIUrl":"10.1016/j.carbpol.2025.123527","url":null,"abstract":"<div><div>Driven by the quest for greener flocculants, this study explores how cationized polysaccharides can enhance coagulation-flocculation efficiency, filling a gap in systematic comparative analyses. We introduce here a flocculation score which provides a robust framework for flocculation evaluation and comparison. Starch, chitosan, inulin, guar gum, pullulan, and hydroxyethyl cellulose were tested, both unmodified and modified with quaternary ammonium compounds. Promising results in coagulation-flocculation were observed compared to synthetic flocculants like cationic polyacrylamide (cPAM) and Poly(Diallyldimethylammonium Chloride) (pDADMAC).</div><div>Advanced techniques such as AsFlFFF-MALS, NMR, PCD, rheology, SEM, image analysis, and zeta potential were used to thoroughly characterize these polysaccharides and their flocculation efficiency. Results revealed notable flocculation enhancement in kaolin suspension (model system) and industrial starch sludge with cationized polysaccharides over their unmodified counterparts. Key influencing factors —molecular weight, charge density, and viscosity— and their trends were identified, with higher charge densities notably enhancing flocculation particularly in kaolin suspensions, and higher molecular weight enhancing flocculation in industrial sludge. Chitosan emerged as the top unmodified polysaccharide, while cationic pullulan and starch were found to lead among modified polysaccharides.</div><div>This study highlights eco-friendly cationized polysaccharides, providing insights for optimizing water treatment and a comparative analysis of six polysaccharides' intrinsic parameters and flocculation efficiency.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123527"},"PeriodicalIF":10.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formation of dimers delayed alginate degradation in fecal microbiota fermentation

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-21 DOI: 10.1016/j.carbpol.2025.123524

Dan Yuan , Wenqian Xiao , Xingyu Tao , Zhiming Gao , Yuehan Wu , Wenxin Jiang , Yanlei Li , Xuewen Ni , Mengzhou Zhou

{"title":"Formation of dimers delayed alginate degradation in fecal microbiota fermentation","authors":"Dan Yuan , Wenqian Xiao , Xingyu Tao , Zhiming Gao , Yuehan Wu , Wenxin Jiang , Yanlei Li , Xuewen Ni , Mengzhou Zhou","doi":"10.1016/j.carbpol.2025.123524","DOIUrl":"10.1016/j.carbpol.2025.123524","url":null,"abstract":"<div><div>The present study was to examine the dimer formation of two alginate chains and its degradation in gut microbiota using an <em>in vitro</em> colon fermentation model. The most rapid degradation stage of guluronate in alginate was from 6 h to 24 h (1.68 μg/mL/h), while guluronate in dimers was from 24 h to 36 h (1.69 μg/mL/h). The degradation extent of alginate (97.40 %) was remarkably higher than that of dimers (84.20 %) in 48 h fermentation. Fecal microbiota randomly cleaved alginate into discrete mannuronate blocks (M-blocks), guluronate blocks (G-blocks), or M/G G-blocks. In contrast, dimers were sequentially cleaved into M-blocks before the crosslinked G-blocks. Approximately 25 % of crosslinked G-blocks survived the 48-h fermentation. Furthermore, three <em>Bacteroides spp.</em> strains could collaboratively degrade M-blocks and then crosslinked G-blocks in dimers sequentially. Collectively, the crosslinked G-blocks impeded the dimer degradation by fecal microbiota, presenting a slower degradation rate and a lesser degradation extent.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123524"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional characteristics of esterified starch and microencapsulation for urate-lowering probiotic: Effect of hydrophobic side-chain length

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-21 DOI: 10.1016/j.carbpol.2025.123529

Huijing Chen , Juxin He , Wanchun Li , Zhirong Wang , Muying Du , Jianquan Kan

{"title":"Structural and functional characteristics of esterified starch and microencapsulation for urate-lowering probiotic: Effect of hydrophobic side-chain length","authors":"Huijing Chen , Juxin He , Wanchun Li , Zhirong Wang , Muying Du , Jianquan Kan","doi":"10.1016/j.carbpol.2025.123529","DOIUrl":"10.1016/j.carbpol.2025.123529","url":null,"abstract":"<div><div>Esterified starch exhibits desirable freeze-thaw stability and emulsifying properties; however, there are limited reports on how the side-chain length of the acid anhydride affects the antifreeze activity and microencapsulation ability of starch. In this study, four types esterified starch with different hydrophobic chains were prepared and their physicochemical characteristics were explored. The results indicated that acid anhydride modification decreased the amylose content and relative crystallinity of native starch and altered its surface composition. Dodecenyl and hexadecenyl succinic anhydrides rendered the starch with relatively low degree of substitution but strong surface hydrophobicity (>120°). The order of anhydrides with different chain lengths to impart emulsifying ability to starch was dodecenyl succinic anhydride > octenyl succinic anhydride > hexadecenyl succinic anhydride > maleic anhydride, whereas the O/W Pickering emulsion storage stability of hexadecenyl succinic anhydride-esterified starch significantly decreased. Moreover, modified starches with superior freeze–thaw stability for microencapsulation improved the freeze-drying resistance of urate-lowering probiotics. Meanwhile, the encapsulated probiotics which passage through a simulated digestion exhibited superior xanthine oxidase inhibition. These results indicate that the appropriate chain-length acid-anhydride-modified starch is a promising cryoprotectant for frozen products.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123529"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multifunctional metformin loaded carboxymethyl chitosan/tannic acid/manganese composite hydrogel with promising capabilities for age-related bone defect repair

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-21 DOI: 10.1016/j.carbpol.2025.123526

Jingle Chen , Chao Xie , Yucong Li , Qili Sun , Fengnian Yu , Kai Li , Haotian Gao , Zhaoquan Liang , Bin Tang , Lijun Lin

{"title":"A multifunctional metformin loaded carboxymethyl chitosan/tannic acid/manganese composite hydrogel with promising capabilities for age-related bone defect repair","authors":"Jingle Chen , Chao Xie , Yucong Li , Qili Sun , Fengnian Yu , Kai Li , Haotian Gao , Zhaoquan Liang , Bin Tang , Lijun Lin","doi":"10.1016/j.carbpol.2025.123526","DOIUrl":"10.1016/j.carbpol.2025.123526","url":null,"abstract":"<div><div>As the global population ages, age-related bone defects have become a major public health challenge. The decline in bone tissue repair capacity among the elderly is primarily attributed to the senescence of bone marrow mesenchymal stem cells (BMSCs), which leads to reduced proliferation and differentiation capabilities, thereby impeding the bone healing process. Additionally, the deterioration of the bone microenvironment, characterized by chronic inflammation and oxidative stress, further complicates bone repair. To address these issues, a multifunctional hydrogel drug delivery system, the metformin-loaded carboxymethyl chitosan/tannic acid/manganese (MCTM) hydrogel was developed. This system integrates the synergistic effects of CMCS, TA, Mn<sup>2+</sup>, and metformin to effectively alleviate BMSCs senescence, optimize the local chronic inflammatory microenvironment, eliminate oxidative stress, and reduce post-implantation infection risks. Detailed material characterization revealed that the introduction of Mn<sup>2+</sup> significantly enhances the mechanical properties and optimizes the degradation characteristics of the CMCS/TA hydrogel, ensuring continuous and stable drug release at tissue repair sites. In vitro and in vivo experiments demonstrated MCTM's excellent biocompatibility and its ability to combine stem cell senescence alleviation with bone repair microenvironment improvement, thereby effectively overcoming various adverse factors affecting bone defect repair in the elderly. This study presents a promising strategy for enhancing bone regeneration under senescent conditions.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123526"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-dynamic crosslinking network enabled strong, flexible, self-healing, and biodegradable chitosan fiber paper/vitrimer composites for plastic substitution

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-21 DOI: 10.1016/j.carbpol.2025.123523

Xiaoqian Zhang , Leyi Lin , Haonan Zhou, Jie Zou, Wang Zhao, Guowen Zhou, Zepeng Lei, Xiaohui Wang

{"title":"A dual-dynamic crosslinking network enabled strong, flexible, self-healing, and biodegradable chitosan fiber paper/vitrimer composites for plastic substitution","authors":"Xiaoqian Zhang , Leyi Lin , Haonan Zhou, Jie Zou, Wang Zhao, Guowen Zhou, Zepeng Lei, Xiaohui Wang","doi":"10.1016/j.carbpol.2025.123523","DOIUrl":"10.1016/j.carbpol.2025.123523","url":null,"abstract":"<div><div>The development of high-performance biomass-based vitrimers has emerged as a crucial research topic to reduce reliance on petroleum-based plastics. Achieving both high strength and toughness is essential for most biomass-based vitrimers, yet these properties typically tend to be mutually exclusive. Here we show a multifunctional composite, CFP/TAV-PI, prepared by integrating an all-natural polyimine vitrimer (TAV-PI) into chitosan fiber paper (CFP) through in-situ polymerization and heat-pressing treatments. The CFP/TAV-PI films, featuring a dual-dynamic crosslinking network of hydrogen bonds and dynamic imine bonds, achieve a tensile strength of 57.93 MPa, elongation at break of 44.66 %, and toughness of 18.00 MJ m<sup>−3</sup>. Additionally, CFP/TAV-PI possess high transparency, self-healing ability, and thermal/chemical stability. Importantly, they are also readily degradable under both chemical and natural conditions. Our findings highlight a novel approach to prepare degradable composites with remarkable strength and flexibility, offering the potential to replace conventional plastic products.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123523"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure–function relationship of the brown seaweed Undaria pinnatifida laminaran: Protein kinase C-mediated mucus secretion and gut barrier restoration

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-21 DOI: 10.1016/j.carbpol.2025.123525

Kit-Leong Cheong , Lin Chen , Si-Yuan Lu , Amanullah Sabir , Jianping Chen , Zhuo Wang , Suresh Veeraperumal , Jude Juventus Aweya , Xian-Qiang Chen , Saiyi Zhong , Karsoon Tan , A.M. Abd El-Aty

{"title":"Structure–function relationship of the brown seaweed Undaria pinnatifida laminaran: Protein kinase C-mediated mucus secretion and gut barrier restoration","authors":"Kit-Leong Cheong , Lin Chen , Si-Yuan Lu , Amanullah Sabir , Jianping Chen , Zhuo Wang , Suresh Veeraperumal , Jude Juventus Aweya , Xian-Qiang Chen , Saiyi Zhong , Karsoon Tan , A.M. Abd El-Aty","doi":"10.1016/j.carbpol.2025.123525","DOIUrl":"10.1016/j.carbpol.2025.123525","url":null,"abstract":"<div><div>Ulcerative colitis is a chronic inflammatory condition of the intestine characterized by mucosal damage and a compromised epithelial barrier. This study explored the protective and therapeutic potential of laminaran derived from the brown seaweed <em>Undaria pinnatifida</em> in promoting mucin secretion and restoring mucosal barrier integrity. Physicochemical analysis revealed laminaran as having a β-(1 → 3)-linked glucose backbone with β-(1 → 6)-linked branches and a molecular weight of 14.41 kDa. In vitro experiments revealed that laminaran enhanced the expression of mucin-related proteins in a lipopolysaccharide-induced LS174T model. Laminaran also upregulated the expression of sulfotransferases, which are essential for mucin sulfation, and promoted vesicular transport by increasing the expression of vesicle-associated membrane protein 8 and synaptosome-associated protein-23, facilitating mucin secretion. These effects are mediated through the protein kinase C (PKC) pathway, which involves PKCα and PKCβII. In an in vivo model, laminaran alleviated dextran sulfate sodium-induced colitis, increasing mucus thickness and overall intestinal barrier function. These results suggest that laminaran is a promising therapeutic agent for treating ulcerative colitis, suggesting a novel approach to restoring the mucosal barrier and reducing intestinal inflammation. This study lays the groundwork for developing laminaran-based treatments for ulcerative colitis and other intestinal diseases associated with epithelial barrier dysfunction.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123525"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-functional edible coatings tailored with nanocellulose for perishable fruits

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-19 DOI: 10.1016/j.carbpol.2025.123520

Lei Zhang , Mei Cui , Huan Tong , Jiaqi Zhang , Qiannuo Li , Xue Gao , Wei Qi , Hon Loong Lam , Renliang Huang , Rongxin Su

{"title":"Multi-functional edible coatings tailored with nanocellulose for perishable fruits","authors":"Lei Zhang , Mei Cui , Huan Tong , Jiaqi Zhang , Qiannuo Li , Xue Gao , Wei Qi , Hon Loong Lam , Renliang Huang , Rongxin Su","doi":"10.1016/j.carbpol.2025.123520","DOIUrl":"10.1016/j.carbpol.2025.123520","url":null,"abstract":"<div><div>Enhancing food security and nutrition remains a significant global challenge. Edible coatings have been demonstrated to preserve highly perishable fruits to reduce food loss, address chronic undernourishment, minimize health concerns, and improve environmental sustainability. However, existing biobased edible coatings face limitations in functionality and oxygen permeation control, hindering their effectiveness in preserving fruits. In this study, we have developed a multi-functional biobased conformal coating using a dipping process incorporating nanocellulose, bee wax, and curcumin. This innovative coating effectively extends the shelf life of fresh fruits by restraining cellular metabolism, minimizing water loss, and preventing microbial contamination. To tailor oxygen permeation, we have proposed a universal coating thickness power law with high accuracy, along with fruit oxygen barrier lines/bands, ensuring the best preservation performance. Our coating is transparent, washable, and biodegradable while also possessing excellent mechanical strength, antibacterial properties, and antioxidant capabilities. These features make it a promising and environmentally friendly solution for enhancing food security.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123520"},"PeriodicalIF":10.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of polysaccharide-based block copolymers obtained by click chemistry from opened β-cyclodextrin and growth polymerization

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-19 DOI: 10.1016/j.carbpol.2025.123521

Dindet Steve Evanes Koffi Teki , Hajeeth Thankappan , Matthieu Pélingre , Mariama Ndour , Cédric Przybylski , Emmanuel Petit , Julien Bernard , Véronique Bonnet , Eric Drockenmuller , José Kovensky

{"title":"Synthesis of polysaccharide-based block copolymers obtained by click chemistry from opened β-cyclodextrin and growth polymerization","authors":"Dindet Steve Evanes Koffi Teki , Hajeeth Thankappan , Matthieu Pélingre , Mariama Ndour , Cédric Przybylski , Emmanuel Petit , Julien Bernard , Véronique Bonnet , Eric Drockenmuller , José Kovensky","doi":"10.1016/j.carbpol.2025.123521","DOIUrl":"10.1016/j.carbpol.2025.123521","url":null,"abstract":"<div><div>The synthesis of well-defined oligosaccharides via cyclodextrin (CD) ring opening is an efficient method for obtaining tailored monomers, which are then suitable for further polymerization. Starting from benzoylated β-CD, which contains seven glucose units, pure difunctionalized benzoylated heptaoses were synthesized. This approach produced heptaoligosaccharides with either azide (A) or propargyl (B) as reactive groups at the reducing and non-reducing ends with a yield between 81 and 96 %, and corresponding to α,ω-diazidoheptaose, α,ω-dipropargylheptaose, and ω-azido-α-propargylheptaose for the AA, BB, and AB monomers, respectively. A highly efficient deprotection process provided access to difunctionalized linear and polar heptasaccharides with high purity (87–99 %). The newly synthesized oligosaccharidic blocks were then polymerized via copper-catalyzed 1,3-dipolar cycloaddition leading to seven original and distinct polymers. These include unprotected (AA-BB or AB-AB) or benzoylated (AB-AB) homo- and hetero-copolymers, as well as hydrophobic blocks randomly distributed with hydrophilic ones (AB-AB) or alternating unprotected-benzoylated blocks (AA-BB). Two additional polymers were obtained by quaternization of triazole rings. Characterization by SEC, TGA, XRD, NMR, and MALDI-TOF MS revealed that a mixture of polymers containing 1–34 blocks could be obtained, with a degree of polymerization ranging from 126 to 238 sugar units and moderate to excellent yields (30–85 %).</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123521"},"PeriodicalIF":10.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative insights into the interaction dynamics and gel properties of myofibrillar protein with structurally diverse pectin

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-19 DOI: 10.1016/j.carbpol.2025.123519

Han Yu, Jian Zhang

{"title":"Innovative insights into the interaction dynamics and gel properties of myofibrillar protein with structurally diverse pectin","authors":"Han Yu, Jian Zhang","doi":"10.1016/j.carbpol.2025.123519","DOIUrl":"10.1016/j.carbpol.2025.123519","url":null,"abstract":"<div><div>This study investigated the interactions between three structurally distinct apple pectin fractions and myofibrillar protein at varying ratios, focusing on their effects on gelation behavior. The optimal ratio of pectin to myofibrillar protein (1:7) facilitated the structural transition of myofibrillar protein from α-helix to β-sheet, improving its thermal stability by 1.2 times. This ratio also enhanced the exposure of sulfhydryl groups, hydrophobic groups, and tryptophan residues, promoting intermolecular chain entanglement. Our results indicate that the molecular weight and degree of esterification of pectin were key determinants of gelation properties. Specifically, the pectin with the highest molecular weight (201.6 kDa) and degree of esterification (75 %) exhibited a 97.3 % higher affinity for myofibrillar protein, as well as the highest cross-linking density with myofibrillar protein, compared to the pectin with the lowest molecular weight (131.6 kDa) and esterification degree (34.9 %). These molecular interactions significantly enhanced water-holding capacity (33.8 % increase) and textural attributes (1044 % increase in hardness and 31.5 % increase in elasticity) relative to the control group. These findings highlight the role of pectin structure in food protein applications, offering potential strategies for designing functional protein-based food systems.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123519"},"PeriodicalIF":10.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First evidence of cryo-milling enhancing enzymatic production of chitooligosaccharides from chitin biomass

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-03-18 DOI: 10.1016/j.carbpol.2025.123509

Saumashish Mukherjee , Stefan Cord-Landwehr , T. Swaroopa Rani , Lal Duhsaki , Sreedhara Sudhakara Sarma , Tata Narsinga Rao , Bruno M. Moerschbacher , Jogi Madhuprakash

{"title":"First evidence of cryo-milling enhancing enzymatic production of chitooligosaccharides from chitin biomass","authors":"Saumashish Mukherjee , Stefan Cord-Landwehr , T. Swaroopa Rani , Lal Duhsaki , Sreedhara Sudhakara Sarma , Tata Narsinga Rao , Bruno M. Moerschbacher , Jogi Madhuprakash","doi":"10.1016/j.carbpol.2025.123509","DOIUrl":"10.1016/j.carbpol.2025.123509","url":null,"abstract":"<div><div>Cryo-milling offers an energy-efficient approach to process α-chitin, the most prevalent and structurally robust chitin form. Unlike ball-milling, cryo-milling operates at low temperatures (<123 K), preventing heat-induced charring and loss of chitooligosaccharide. This study investigated the effects of various cryo-milling conditions on α-chitin, resulting in reduced crystalline plane intensity, increased surface area, and smaller particle size without causing substrate breakdown or functional group loss. Additionally, the study investigated cryo-milled α-chitin's enzymatic hydrolysis for the first time. Using <em>Paenibacillus</em> sp. LS1's chitin-active-secretome, cryo-milled α-chitin yielded twice as much GlcNAc compared to unmilled α-chitin. Hydrolysis with recombinant Chi5 from <em>Paenibacillus</em> sp. LS1 increased (GlcNAc)<sub>2</sub> and GlcNAc yields by twofold and fourfold, respectively, while recombinant <em>Str</em>ChiA from <em>Streptomyces</em> sp. UH6 led to an elevenfold and twofold increase, respectively. Overall, cryo-milling emerges as a gentle yet effective alternative to ball-milling, significantly enhancing enzymatic saccharification of α-chitin by increasing surface area, thereby optimizing chitooligosaccharide production.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"358 ","pages":"Article 123509"},"PeriodicalIF":10.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0