Carbohydrate Polymers最新文献_第3页

Cellulose-based aerogels modulate fragrance adsorption and controlled release by carbonization/in-situ aromatization 纤维素基气凝胶通过炭化/原位芳构化调节香味吸附和控释

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-14 DOI: 10.1016/j.carbpol.2025.124061

Tingyi Zhao , Qidong Zhang , Ke Zhang , Qingzhao Shi , Menglan Xiao , Mingqin Zhao , Miao Lai , Bing Cui

{"title":"Cellulose-based aerogels modulate fragrance adsorption and controlled release by carbonization/in-situ aromatization","authors":"Tingyi Zhao , Qidong Zhang , Ke Zhang , Qingzhao Shi , Menglan Xiao , Mingqin Zhao , Miao Lai , Bing Cui","doi":"10.1016/j.carbpol.2025.124061","DOIUrl":"10.1016/j.carbpol.2025.124061","url":null,"abstract":"<div><div>Fragrances are indispensable additives in consumer products including foods, cosmetics, and tobacco products. However, their inherent instability leads to rapid quality degradation and performance loss, driving the urgent need for controlled-release systems to stabilize fragrance performance. In this work, cellulose nanofibers (CNF) were used to prepare CNF aerogel-like gels (CA) and carbonized CNF aerogels (C-CA) through freeze-drying and high-temperature carbonization. Two model fragrance molecules such as <span>d</span>-limonene (DL) and methyl dihydrojasmonate (MD) were loaded into the aerogels and aerogel-like gels using vapor-phase adsorption and in-situ method. The study systematically evaluated how material types, loading methods, fragrance properties influence adsorption and controlled-release performance. The results demonstrate that the unique porous structure of the materials and the formation of hydrogen bonds contribute to the adsorption and controlled release of fragrance molecules. The fragrance-loaded aerogels and aerogel-like gels achieved tunable loading capacities ranging from 10.04 to 578.09 mg/g. Temperature-controlled experiments demonstrated adjustable fragrance release percentages, enabling the selection of tailored aerogel materials for specific application environments in controlled-release systems. These findings advance the simple design of CNF-based aerogels and aerogel-like gels delivery systems for precise fragrance control.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124061"},"PeriodicalIF":10.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A robust method for quantitative detection of β-1,3-glucan using Limulus polyphemus factor Gα fragment 用鲎因子Gα片段定量检测β-1,3-葡聚糖的可靠方法

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-14 DOI: 10.1016/j.carbpol.2025.124060

Andong Zhou , Yitong Li , Yuanning Wang , Yibing Wang , Zhonghui Qiao , Kevin H. Mayo , Zihan Zhao , Lin Sun

{"title":"A robust method for quantitative detection of β-1,3-glucan using Limulus polyphemus factor Gα fragment","authors":"Andong Zhou , Yitong Li , Yuanning Wang , Yibing Wang , Zhonghui Qiao , Kevin H. Mayo , Zihan Zhao , Lin Sun","doi":"10.1016/j.carbpol.2025.124060","DOIUrl":"10.1016/j.carbpol.2025.124060","url":null,"abstract":"<div><div>β-1,3-glucan is an important glycan in the cell wall of most fungi and plants. Accurate detection of β-1,3-glucans is essential for their use in the food and pharmaceutical industries. Here, we developed a robust method to detect β-1,3-glucan using <em>Limulus polyphemus</em> factor Gα (LFGα) fragments. Five β-1,3-glucans with various degrees of branching were extracted from various edible mushrooms and structurally characterized. Their binding affinities to truncated LFGα variants (Q + D1 + D2, D1 + D2, D1, D2) were evaluated using the ELISA assay. LFGα fragment Q + D1 + D2 exhibited the highest affinity toward all β-1,3-glucans regardless of the degree of branching, whereas fragments D1 + D2, D1, and D2 had reduced affinities as the degree of branching increased. AlphaFold-predicted models and molecular docking supported these findings. The branching-insensitive binding with Q + D1 + D2 enabled us to accurately quantify the amount of β-1,3-glucan using a Q + D1 + D2-based ELISA assay. A standard curve was established using commercial curdlan, and quantitative detection of five mushroom β-1,3-glucans was in agreement with their actual glucan content. Our results demonstrate that the Q + D1 + D2-based ELISA assay can be developed as an efficient method for quantitative detection of β-1,3-glucans.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"366 ","pages":"Article 124060"},"PeriodicalIF":10.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NIR-responsive dextran / poly(lactide) hydrogels: Characterization of cleavable hydrogels and photoactivated release of proteins nir响应右旋糖酐/聚丙交酯水凝胶：可切割水凝胶的表征和蛋白质的光激活释放

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-14 DOI: 10.1016/j.carbpol.2025.124068

Mickael Guerreiro , Anissa Benkhedim , Doriane Voisin , Clémence Farine , Audrey Bethry , Cédric Paniagua , Hélène Van Den Berghe , Benjamin Nottelet

{"title":"NIR-responsive dextran / poly(lactide) hydrogels: Characterization of cleavable hydrogels and photoactivated release of proteins","authors":"Mickael Guerreiro , Anissa Benkhedim , Doriane Voisin , Clémence Farine , Audrey Bethry , Cédric Paniagua , Hélène Van Den Berghe , Benjamin Nottelet","doi":"10.1016/j.carbpol.2025.124068","DOIUrl":"10.1016/j.carbpol.2025.124068","url":null,"abstract":"<div><div>Polysaccharide-based hydrogels often lack mechanical strength and, when used for protein delivery, are generally limited to diffusion-based release. In this work, we developed robust polysaccharide- and polyester-based near-infrared (NIR)-responsive hydrogels. Hydrogels are made from photo-crosslinked methacrylated dextran (DEX-MA), methacrylated polylactide containing oxygen reactive species (ROS) sensitive thioketal groups (PLA-TK-MA), and covalently bound protoporphyrin IX (PPIX) that generates ROS under NIR irradiation. We investigated the effects of DEX methacrylation degree (3, 12 and 45 %), PLA molecular weight (2500 vs. 5000 g.mol<sup>−1</sup>) and DEX/PLA ratios (95/5 to 50/50) on the gels' rheological properties. We then examined the NIR-sensitivity of selected hydrogels by comparing their water uptakes and storage/loss moduli. Finally, we studied the release of a model protein, bovine serum albumin (BSA), from the hydrogels with and without NIR-irradiation. Results confirmed that protein release kinetics can be controlled by NIR-irradiation, with faster and complete release of BSA compared to non-irradiated samples.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124068"},"PeriodicalIF":10.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Lactobacillus plantarum fermentation on the retrogradation behaviors, physicochemical properties and structure of rice starch 植物乳杆菌发酵对水稻淀粉降解行为、理化性质及结构的影响

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-14 DOI: 10.1016/j.carbpol.2025.124064

Jingwen Luo , Wafaa Khalid , Li Wang , Yan Li , Mingcong Fan , Haifeng Qian

{"title":"Effects of Lactobacillus plantarum fermentation on the retrogradation behaviors, physicochemical properties and structure of rice starch","authors":"Jingwen Luo , Wafaa Khalid , Li Wang , Yan Li , Mingcong Fan , Haifeng Qian","doi":"10.1016/j.carbpol.2025.124064","DOIUrl":"10.1016/j.carbpol.2025.124064","url":null,"abstract":"<div><div>Starch retrogradation critically compromises shelf stability in rice-based products. This study demonstrates <em>Lactobacillus plantarum</em> (LP) fermentation as an effective biological strategy to retard retrogradation in japonica (JRS), indica (IRS), and glutinous (GRS) rice starches. Controlled fermentation (0–48 h) followed by 4 °C storage (0–14 d) induced significant structural and functional modifications. Results revealed that 48 h LP fermentation significantly suppressed retrogradation, with JRS, IRS and GRS exhibiting 27.55 %, 26.45 %, 35.12 % reductions in retrogradation degree after 14 d of storage compared to unfermented controls (<em>P</em> < 0.05), underscoring LP's broad efficacy. Following 14 days of storage, both JRS and IRS demonstrated significant reductions in crystallinity (38.51 % and 22.56 %, respectively) and short-range molecular order (R<sub>1047/1022</sub> decreased by 8.18 % and 11.58 %, respectively) when compared to unfermented controls. The diminished crystallinity and disrupted short-range molecular order in the fermented starches were correlated with delayed recrystallization kinetics. Collectively, these structural modifications inhibited retrogradation. Our findings demonstrate that LP fermentation serves as a natural and effective strategy to enhance functional stability in rice-based foods, with promising applications in ready-to-eat meals and gluten-free baked goods.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124064"},"PeriodicalIF":10.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking phase transitions of tactoids in sulfated cellulose nanocrystals using second harmonic generation microscopy 利用二次谐波发生显微镜跟踪硫酸盐纤维素纳米晶体中微球的相变

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-13 DOI: 10.1016/j.carbpol.2025.124062

Thibaut Legat , Olivier Deschaume , Carmen Bartic , Yovan de Coene , Wim Thielemans , Stijn Van Cleuvenbergen

{"title":"Tracking phase transitions of tactoids in sulfated cellulose nanocrystals using second harmonic generation microscopy","authors":"Thibaut Legat , Olivier Deschaume , Carmen Bartic , Yovan de Coene , Wim Thielemans , Stijn Van Cleuvenbergen","doi":"10.1016/j.carbpol.2025.124062","DOIUrl":"10.1016/j.carbpol.2025.124062","url":null,"abstract":"<div><div>Cellulose nanocrystals (CNCs) have emerged as promising candidates for chiroptical functional materials due to their ability to form cholesteric liquid crystals with tunable periodicity. The quality of the final cholesteric phase is influenced by the nucleation, growth and coalescence mechanism of the initial droplets, known as tactoids. Current research focuses on understanding the size and morphological transformations of these tactoids, to gain deeper insights into their dynamic behavior and, in turn, to better control the final properties of novel photonic materials. For theoretical models to effectively describe these transitions, precise measurement of these geometric quantities is critical. While polarized microscopy is commonly used, it struggles to accurately measure volumes and aspect ratios. Here, we demonstrate how second-harmonic generation (SHG) microscopy can be used to visualize tactoids with unprecedented detail, offering three-dimensional representations of their size, morphology, and fusion processes in a single volumetric scan. Additionally, the fully cholesteric phase of the suspension can be imaged with SHG to identify potential defects and correlate them with the fusion process of tactoids; specifically, with how the tactoids fuse and merge along the chiral axis, thus linking early tactoid events to the overall quality of the final cholesteric phase.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124062"},"PeriodicalIF":10.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analyses of thermal transport in cellulose at crystalline, paracrystalline and amorphous states 纤维素在晶态、准晶态和非晶态的热传递比较分析

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-13 DOI: 10.1016/j.carbpol.2025.124063

Yichen Hou , Ruo-Yu Dong

{"title":"Comparative analyses of thermal transport in cellulose at crystalline, paracrystalline and amorphous states","authors":"Yichen Hou , Ruo-Yu Dong","doi":"10.1016/j.carbpol.2025.124063","DOIUrl":"10.1016/j.carbpol.2025.124063","url":null,"abstract":"<div><div>Cellulose exhibits distinct phase characteristics across its crystalline, paracrystalline, and amorphous states. While prior studies have established correlations between condensed states and thermal properties in inorganic materials, the fundamental mechanisms governing heat transfer in polymers, particularly regarding phase transformations, remain poorly understood. Through molecular dynamics simulations, we quantitatively characterize the progressive loss of long-range order during crystalline-paracrystalline-amorphous transition of cellulose and elucidate its profound impact on thermal transport suppression. Our in-depth phonon analyses reveal synergistic mechanisms driving this reduction: diminished mid-frequency phonon contributions, enhanced phonon localization, intensified scattering processes, and reduced phonon lifetimes and group velocities. This work sheds light on the fundamental mechanisms of thermal transport during polymer phase transformations and offers valuable insights for the design and application of cellulose-based functional materials, particularly in thermal management applications.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"367 ","pages":"Article 124063"},"PeriodicalIF":10.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advance in the extraction, characterization, bioactivities, and application prospects of konjac oligosaccharide: A comprehensive review 魔芋低聚糖的提取、表征、生物活性及应用前景综述

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-13 DOI: 10.1016/j.carbpol.2025.124053

Xiaoyan Chang , Yi Han , Cuiping Yang , Liyuan Gao , Zhuoyi Wang , Ying Meng , Min Zhang , Xiang Yang , Zhitao Jiang

{"title":"Advance in the extraction, characterization, bioactivities, and application prospects of konjac oligosaccharide: A comprehensive review","authors":"Xiaoyan Chang , Yi Han , Cuiping Yang , Liyuan Gao , Zhuoyi Wang , Ying Meng , Min Zhang , Xiang Yang , Zhitao Jiang","doi":"10.1016/j.carbpol.2025.124053","DOIUrl":"10.1016/j.carbpol.2025.124053","url":null,"abstract":"<div><div>Konjac has garnered significant attention for its dual roles in medicinal and nutritional applications, with konjac oligosaccharide (KOS) emerging as a bioactive compound of particular interest. KOS exhibits a wide range of biological activities, including laxative, immunomodulatory, glucolipid metabolism regulatory, antioxidative, antifatigue, anti-inflammatory, osteogenic, and prebiotic effects. Despite advances in its extraction, purification, and characterization, a deeper understanding of its structural properties and mechanisms of action remains essential. This review systematically examines the extraction, separation, and purification techniques of KOS, alongside its physicochemical properties, biological activities, structure-activity relationships, and potential applications. Additionally, the toxicity and safety profile of KOS are critically evaluated. By integrating these aspects, this review provides theoretical and technical insights to support the development of KOS-based functional foods, highlighting its potential in the food and nutraceutical industries.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"367 ","pages":"Article 124053"},"PeriodicalIF":10.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulose nanofibers as effective mediator for optical chemical sensors for heavy metal ions detection 纤维素纳米纤维作为重金属离子检测光学化学传感器的有效介质

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-12 DOI: 10.1016/j.carbpol.2025.124028

Mahsa Mousavi Langari , Federica Mandoj , Giuseppe Pomarico , M. Mirari Antxustegi , Roberto Paolesse , Jalel Labidi , Larisa Lvova

{"title":"Cellulose nanofibers as effective mediator for optical chemical sensors for heavy metal ions detection","authors":"Mahsa Mousavi Langari , Federica Mandoj , Giuseppe Pomarico , M. Mirari Antxustegi , Roberto Paolesse , Jalel Labidi , Larisa Lvova","doi":"10.1016/j.carbpol.2025.124028","DOIUrl":"10.1016/j.carbpol.2025.124028","url":null,"abstract":"<div><div>Two forms of nanocellulose-based sensing materials were developed for heavy metal ions (HMIs) detection: all-solid-state and suspension. In these materials, cellulose nanofibers (CNF), isolated from cellulose bleached pulp via homogenization, were employed as a support matrix. For all-solid-state optodes development free-base 5,10,15,20-tetraphenylporphyrin (TPP) and zinc-porphyrin derivative (ZnPC) were deposited on CNF support. Moreover, CNF suspension enriched with silver nanoparticles (AgNPs) bearing 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin (TPP(SO<sub>3</sub>)<sub>4</sub>) was tested for HMIs assessment in solution. The solid-state platform enabled fast and low-cost optical detection of multiple HMIs in individual and multicomponent solutions; a smartphone digital camera was used for optical signal acquisition, while chemometric methods were employed for data analysis. Among tested HMIs, the highest attention was focused on Cd<sup>2+</sup> and Pb<sup>2+</sup> ions detection, for which the low detection limit (LDL) was estimated 0.0018 and 0.0033 mgL<sup>−1</sup>, respectively. These values are lower than the WHO provisional guideline values of 0.003 and 0.01 mgL<sup>−1</sup> for natural waters. CNF suspension was characterized by SEM, IR and UV–vis spectroscopy, showing a successful loading of TPP(SO<sub>3</sub>)<sub>4</sub> and incorporation of AgNPs into CNF. The effective assessment of HMIs in individual solutions through the naked eye detection, and by UV–vis tests demonstrate the potential of CNF-based sensing materials for environmental monitoring applications.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124028"},"PeriodicalIF":10.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering robust bio-based 3D structures from corn stalk pith through a sustainable route to nano-micro structured extreme environment resistant materials 工程稳健的生物基3D结构，从玉米秸秆髓通过可持续的途径到纳米-微观结构的极端环境抗性材料

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-12 DOI: 10.1016/j.carbpol.2025.124054

Zhenpeng Shen , Yantao Wang , Huili Wang, Ruidong Chu, Dehai Yu, Zhaoping Song, Guodong Li, Xiaona Liu, Wenxia Liu

{"title":"Engineering robust bio-based 3D structures from corn stalk pith through a sustainable route to nano-micro structured extreme environment resistant materials","authors":"Zhenpeng Shen , Yantao Wang , Huili Wang, Ruidong Chu, Dehai Yu, Zhaoping Song, Guodong Li, Xiaona Liu, Wenxia Liu","doi":"10.1016/j.carbpol.2025.124054","DOIUrl":"10.1016/j.carbpol.2025.124054","url":null,"abstract":"<div><div>Porous three-dimensional (3D) structures with excellent resilience have attracted considerable attention across various fields, including construction and energy storage. However, current fabrication methods often rely on bottom-up approaches, leading to complex production processes and environmental concerns. In this work, we develop the bio-based 3D structures that preserves the intrinsic cellular microstructure of biomass <em>via</em> cell wall engineering under mild conditions. Corn stalk pith (CSP), an agricultural waste, is sequentially treated with dilute acid and an inorganic salt solvent, resulting in the 3D structures with excellent compressive resilience, even at a strain of 70 % in extreme environments. The enhanced compressive resilience is primarily attributed to the reduction of rigid lignin content, the intrinsic cellular microstructure, and the thickened hydrogel cell wall. Metal ions introduced through the salt solvent conferred ionic conductivity to the material, enabling a detection limit as low as 0.1 kPa. These properties allow the bio-based sensor to precisely monitor various human activities by translating compressive deformation into reliable signals. This work presents an innovative strategy for creating naturally porous and elastic materials with exceptional structural stability using agricultural wastes. These materials hold potential applications in oil-water separation, flexible electronics, and thermal insulation.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"367 ","pages":"Article 124054"},"PeriodicalIF":10.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the mechanism of enzymatic resistance in different high amylose starch granules 揭示不同高直链淀粉颗粒的酶抗性机制

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2025-07-12 DOI: 10.1016/j.carbpol.2025.124052

Yu Tian , Yu Wang , Xingxun Liu , Peter Westh , Marie S. Møller , Kim Henrik Hebelstrup , Birte Svensson , Andreas Blennow , Yuyue Zhong

{"title":"Unravelling the mechanism of enzymatic resistance in different high amylose starch granules","authors":"Yu Tian , Yu Wang , Xingxun Liu , Peter Westh , Marie S. Møller , Kim Henrik Hebelstrup , Birte Svensson , Andreas Blennow , Yuyue Zhong","doi":"10.1016/j.carbpol.2025.124052","DOIUrl":"10.1016/j.carbpol.2025.124052","url":null,"abstract":"<div><div>This study investigates the phenomenon that, in contrast to amylopectin-rich starch granules, high amylose starch (HAS) granules typically exhibit high hydrolytic resistance manifested as low density of enzyme attack sites on the starch granule surface. However, among the various types of examined HAS granules, we identified differences in enzymatic resistance. We associated this effect as a result of variations in specific rate of the enzymatic reaction, with intermediate affinity leading to the highest enzymatic efficacy characteristic for the Sabatier principle. However, a surprisingly low hydrolytic resistance, in fact the lowest among the investigated HAS, was found for amylose-only barley starch granules (97 % amylose). This behavior was connected with an optimal affinity of the applied model hydrolase, glucoamylase (GA), leading to the highest hydrolytic efficacy among the HASs. Exploration of surface structures by Fourier transform infrared (FTIR) spectroscopy revealed that larger starch granules with less ordered surface structures tended to exhibit optimum affinity for GA, and hence get degraded with higher catalytic activity and being less resistant. The findings improve our perception of the mechanisms that underlie hydrolytic enzyme resistance of HASs, providing implications for understanding and rationally design of enzymatically modified resistant starches.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124052"},"PeriodicalIF":10.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0