新型丝素/壳聚糖微凝胶用于增强益生菌的输送:改善胃肠道条件下的稳定性、活力和靶向释放。

IF 12.5 1区 化学 Q1 CHEMISTRY, APPLIED
Carbohydrate Polymers Pub Date : 2025-11-15 Epub Date: 2025-08-07 DOI:10.1016/j.carbpol.2025.124191
Zhu Zeng, Tianhao Wang, Yi Yang, Yexuan He, Shuwen Deng, Fangyin Dai, Xiaoling Tong
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引用次数: 0

摘要

本研究以丝素蛋白(SF)和壳聚糖(CS)为基础,制备了一种新型复合微凝胶,用于包封副干酪乳杆菌和戊糖Pediococcus。通过乳化和转谷氨酰胺酶(TGase)催化凝胶反应制备微凝胶,使SF和CS之间实现共价交联。微观结构表征证实了益生菌细胞在均匀球形基质内的成功包封。傅里叶变换红外光谱(FTIR)显示SF和CS之间形成了异肽和氢键。这些相互作用有助于增强微凝胶的机械强度。差示扫描量热法(DSC)表明,与单独的SF或CS组分相比,微凝胶具有优越的热稳定性。由于CS、SF和SF衍生水解肽(SFPs)的协同自由基清除作用,微凝胶表现出显著的抗氧化性能,它们共同保护被封装的益生菌免受氧化损伤。耐热性和长期储存稳定性的评估显示,在巴氏灭菌和延长储存下,益生菌的活力显著提高。此外,微凝胶有效地保护益生菌免受模拟胃酸(pH 2.0)和胆汁盐的侵害,同时使益生菌在肠道中可控释放(约90%的益生菌在模拟肠液中释放)。这些发现表明,基于SF/ cs的微凝胶在食品应用中具有更高的稳定性、生物利用度和功能性,是一种很有前景的益生菌递送平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Novel silk fibroin/chitosan microgel for enhanced probiotic delivery: Improved stability, viability, and targeted release in gastrointestinal conditions.

Herein, a novel hybrid microgel based on silk fibroin (SF) and chitosan (CS) was developed for encapsulating Lactobacillus paracasei and Pediococcus pentosaceus. The microgel was fabricated via emulsification and transglutaminase (TGase)-catalyzed gelation, enabling covalent crosslinking between SF and CS. Microstructural characterization confirmed the successful encapsulation of probiotic cells within homogeneous spherical matrices. Fourier transform infrared (FTIR) spectra showed the formation of isopeptide and hydrogen bonds between SF and CS. These interactions contributed to the enhanced mechanical strength of the microgel. Differential scanning calorimetry (DSC) demonstrated superior thermal stability of the microgels compared with individual SF or CS components. The microgel exhibited notable antioxidant properties owing to the synergistic free radical-scavenging effects of CS, SF, and SF-derived hydrolyzed peptides (SFPs), which collectively protected encapsulated probiotics from oxidative damage. Assessments of thermal resistance and long-term storage stability revealed significant improvements in probiotic viability under pasteurization and extended storage. Furthermore, the microgel effectively protected the probiotics against simulated gastric acid (pH 2.0) and bile salts while enabling their controlled release in the intestine (>90 % probiotics released in simulated intestinal fluid). These findings suggest that the SF/CS-based microgel is a promising delivery platform for probiotics, with enhanced stability, bioavailability, and functionality in food applications.

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来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
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