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引用次数: 0
摘要
传统上,艾叶的根被认为是一种潜在的免疫低下治疗剂,而多糖被认为是其免疫活性的关键物质。本研究从艾叶的根中分离得到了两种新系列菊聚糖(AARP-1和AARP-2)。结果表明,AARP-1和AARP-2的分子量分别为5.46 kDa和3.21 kDa。这两种多糖主要由果糖组成,并表现出特定的骨干键,包括2-β- d - fruf, 1-α- d - glcp和1,2-β- d - fruf -。此外,AARP-1和AARP-2可以通过TLR4/MAPK信号激活,显著促进RAW264.7细胞的增殖和吞噬活性,以及细胞因子的分泌。两种多糖均可通过逆转体重减轻、白细胞减少、清除指数和免疫器官指数来减轻环磷酰胺引起的小鼠免疫抑制。这些结果对AARP-1和AARP-2的结构特性和免疫学作用提供了有价值的见解,强调了它们作为免疫治疗和相关生物医学应用的有前途的天然化合物的潜力。
Structural characterization and immunomodulatory activity of polysaccharides from the roots of Artemisia argyi.
The root of Artemisia argyi is traditionally utilized as a potential therapeutic agent for hypoimmunity and polysaccharides are considered as key substances for its immune activity. In this study, two inulin neoseries-type fructans (AARP-1 and AARP-2) were isolated from the root of A. argyi. The findings revealed that the molecular weights of AARP-1 and AARP-2 were 5.46 kDa and 3.21 kDa, respectively. Both polysaccharides predominantly consisted of fructose and exhibited specific backbone linkages including 2-β-D-Fruf, 1-α-D-Glcp, and 1,2-β-D-Fruf-. In addition, AARP-1 and AARP-2 could markedly promote the proliferation and phagocytic activity, as well as cytokine secretion in RAW264.7 cells via TLR4/MAPK signaling activation. Both polysaccharides alleviated cyclophosphamide-induced immunosuppression in mice by reversing weight loss, leukopenia, clearance index and immune organ index. These results offer valuable insights into the structural properties and immunological roles of AARP-1 and AARP-2, emphasizing their potential as promising natural compounds for immunotherapeutic and related biomedical applications.
期刊介绍:
Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience.
The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.