Surface soft patch-mediated supramolecular co-assembly of lysozyme/konjac glucomannan with optimized antimicrobial performance

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-07-16 DOI:10.1016/j.carbpol.2025.124072

Shanglin Li , Yulun Wu , Yajuan Li , Longjiang Tian , Ting Zhang , Jingbo Liu , Zhiyang Du

{"title":"Surface soft patch-mediated supramolecular co-assembly of lysozyme/konjac glucomannan with optimized antimicrobial performance","authors":"Shanglin Li , Yulun Wu , Yajuan Li , Longjiang Tian , Ting Zhang , Jingbo Liu , Zhiyang Du","doi":"10.1016/j.carbpol.2025.124072","DOIUrl":null,"url":null,"abstract":"<div><div>Assembly is a natural process where molecules spontaneously form ordered structures with specific functions. In supramolecular co-assembly, interactions between surface soft patches drive structural changes, though this key aspect is often overlooked. In this study, we propose a strategy for supramolecular co-assembly mediated by surface soft patches and apply this approach to optimize the broad-spectrum antibacterial activity of lysozyme (LYS)-konjac glucomannan (KGM) supramolecular co-assembly. Surface charge distribution revealed distinct soft patches on LYS and KGM, with hydrogen bond and hydrophobic interactions between them driving supramolecular co-assembly. Characterized by a densely packed β-sheet structure, the supramolecular co-assembly exhibited excellent rheological properties and significantly enhanced antibacterial activity across a broad spectrum. Specifically, the antibacterial efficacy against <em>Staphylococcus aureus</em> exceeded 99.94 %, and that against <em>Escherichia coli</em> reached over 99.95 % in the Ca<sup>2+</sup>-KGM-LYS coacervate. This research sheds new light on supramolecular co-assembly mechanisms and paves the way for designing broad-spectrum antibacterial materials.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"368 ","pages":"Article 124072"},"PeriodicalIF":10.7000,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Polymers","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0144861725008574","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Assembly is a natural process where molecules spontaneously form ordered structures with specific functions. In supramolecular co-assembly, interactions between surface soft patches drive structural changes, though this key aspect is often overlooked. In this study, we propose a strategy for supramolecular co-assembly mediated by surface soft patches and apply this approach to optimize the broad-spectrum antibacterial activity of lysozyme (LYS)-konjac glucomannan (KGM) supramolecular co-assembly. Surface charge distribution revealed distinct soft patches on LYS and KGM, with hydrogen bond and hydrophobic interactions between them driving supramolecular co-assembly. Characterized by a densely packed β-sheet structure, the supramolecular co-assembly exhibited excellent rheological properties and significantly enhanced antibacterial activity across a broad spectrum. Specifically, the antibacterial efficacy against Staphylococcus aureus exceeded 99.94 %, and that against Escherichia coli reached over 99.95 % in the Ca²⁺-KGM-LYS coacervate. This research sheds new light on supramolecular co-assembly mechanisms and paves the way for designing broad-spectrum antibacterial materials.

Abstract Image

查看原文本刊更多论文

表面软贴片介导的溶菌酶/魔芋葡甘露聚糖的超分子共组装及其优化抗菌性能

组装是分子自发形成具有特定功能的有序结构的自然过程。在超分子共组装中，表面软补丁之间的相互作用驱动结构变化，尽管这一关键方面经常被忽视。本研究提出了一种由表面软补丁介导的超分子共组装策略，并应用该策略优化了溶菌酶(LYS)-魔芋葡甘露聚糖（KGM）的超分子共组装的广谱抗菌活性。LYS和KGM表面电荷分布呈现明显的软斑块，它们之间的氢键和疏水相互作用驱动了超分子共组装。该超分子共组装物具有致密的β片结构，具有优异的流变性能，并在广谱范围内显著增强了抗菌活性。其中，Ca2+-KGM-LYS凝聚体对金黄色葡萄球菌的抑菌效果超过99.94%，对大肠杆菌的抑菌效果达到99.95%以上。该研究揭示了超分子共组装机制，为设计广谱抗菌材料铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.