International Journal of Biological Macromolecules最新文献

Tannic-sodium ligninsulfonate self-assembled nanosphere enhanced hydrogel: A stretchable, antibacterial, and all-weather sensor for signal monitoring 单宁-木质素绝缘钠自组装纳米球增强水凝胶：用于信号监测的可拉伸、抗菌和全天候传感器

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-10 DOI: 10.1016/j.ijbiomac.2025.148215

Hongrui Ma , Yutong Yang , Congyu Hou , Yushan Zou , Yachong Zhu , Yingjie Qiao , Yongming Song

{"title":"Tannic-sodium ligninsulfonate self-assembled nanosphere enhanced hydrogel: A stretchable, antibacterial, and all-weather sensor for signal monitoring","authors":"Hongrui Ma , Yutong Yang , Congyu Hou , Yushan Zou , Yachong Zhu , Yingjie Qiao , Yongming Song","doi":"10.1016/j.ijbiomac.2025.148215","DOIUrl":"10.1016/j.ijbiomac.2025.148215","url":null,"abstract":"<div><div>Poly(acrylic acid) (PAA) hydrogels are widely adopted in strain sensors due to their intrinsic ionic conductivity and tunable compliance, yet conventional PAA systems remain brittle (elongation at break typically <100 %), undergo rapid dehydration (>30 % weight loss within 7 days), and lack antibacterial functionality. Here, we report a molecular engineering strategy that integrates self-assembled tannic acid–lignosulfonate (TL) nanospheres with Li<sup>+</sup>/ethylene glycol (EG) co-doping to synergistically reinforce PAA hydrogels. TL nanospheres provide dynamic crosslinking via hydrogen bonding and π–π stacking, Li<sup>+</sup> imparts reversible ionic coordination, while EG forms hydration-stabilizing hydrogen-bond networks. The resulting hydrogel achieves 678.84 % elongation at break (5–7× higher than typical PAA hydrogels), toughness of 2.48 MJ/m<sup>3</sup> (one order of magnitude above reported systems), 87.8 % weight retention after 35 days at 57 % RH (vs. <70 % for conventional hydrogels), and > 85 % antibacterial efficiency against <em>E. coli</em> and <em>S. aureus</em>. Furthermore, it exhibits high ionic conductivity (2.39 S/m) and sensitivity (gauge factor, GF = 8.21), outperforming most PAA-based hydrogels. Integrated with a commercial AD8232 chip, the hydrogel enabled reliable ECG monitoring with fast response (130 ms) and excellent cyclic durability (1000 cycles). This study establishes a generalizable design blueprint that couples mechanical, environmental, and antimicrobial robustness, advancing the development of durable, all-weather wearable biosensors.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148215"},"PeriodicalIF":8.5,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substrate as architect: lignocellulose structures directed assembly lines for humic acid stability in composting 作为建筑师的基质：木质纤维素结构指导装配线在堆肥中腐植酸的稳定性

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-10 DOI: 10.1016/j.ijbiomac.2025.148209

Xingyu Qiao , Jinghan Zhao , Xiaolei Ding , Chunhao Zhang , Fuli Huang , Zonglin Li , Junqiu Wu

{"title":"Substrate as architect: lignocellulose structures directed assembly lines for humic acid stability in composting","authors":"Xingyu Qiao , Jinghan Zhao , Xiaolei Ding , Chunhao Zhang , Fuli Huang , Zonglin Li , Junqiu Wu","doi":"10.1016/j.ijbiomac.2025.148209","DOIUrl":"10.1016/j.ijbiomac.2025.148209","url":null,"abstract":"<div><div>This study established that lignocellulose source dictated distinct humic acid (HA) assembly pathways during composting. We employed an integrated approach combining multi-omics, multispectral analysis, enzyme activity assays, and microbial co-occurrence network modeling to elucidate the humification processes of pine branches (PB), rice straw (RS), and willow branches (WB). A novel metric, the Aromatic Condensation Index (ACI), was developed to quantify the degree of fused aromatic ring structures in HA. Our results revealed divergent humification mechanisms: PB-derived HA exhibited the highest aromatic condensation (ACI = 0.82), facilitated by a tightly clustered microbial community that enabled simultaneous degradation and aromatization. This synergy facilitated biotic (laccase/peroxidase-driven β-O-4 cleavage liberating radicals for C<img>C coupling) and abiotic (Schiff base polymerization of lignin-derived aldehydes and amino acids) pathways. RS composting achieved rapid humification but had low aromatization (ACI = 0.51), attributed to its compartmentalized microbial community decoupling biodegradation from abiotic assembly. Structurally simple RS favored early carbohydrate depolymerization followed by abiotic Maillard reactions. WB composting showed delayed humification and minimal aromatization (ACI = 0.38), with a fragmented microbial network indicating bottlenecks. The recalcitrant, sulfur-rich lignin in WB restricted enzymatic access, necessitating late-stage MnP-mediated demethoxylation; HA formation relied primarily on abiotic “polysaccharides → organic acids → alkyl-HA” assembly. This framework, highlighting lignocellulose structure's role in HA assembly, offers insights into lignin macromolecule degradation and transformation. It provides strategies for efficient lignin utilization, converting it into valuable HA, advancing lignin - based research.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148209"},"PeriodicalIF":8.5,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphate carboxymethyl cellulose nanocomposites with ketoconazole from eggshell waste as a drug delivery platform for odontology application 蛋壳废弃物中磷酸羧甲基纤维素-酮康唑纳米复合材料在口腔医学中的应用

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-10 DOI: 10.1016/j.ijbiomac.2025.148157

Jhonatan do Amparo Madureira , Micheli de Souza Bernardes , Shakeel Abbas , Silas Silva Santana , Fernanda G.L. Medeiros Borsagli

{"title":"Phosphate carboxymethyl cellulose nanocomposites with ketoconazole from eggshell waste as a drug delivery platform for odontology application","authors":"Jhonatan do Amparo Madureira , Micheli de Souza Bernardes , Shakeel Abbas , Silas Silva Santana , Fernanda G.L. Medeiros Borsagli","doi":"10.1016/j.ijbiomac.2025.148157","DOIUrl":"10.1016/j.ijbiomac.2025.148157","url":null,"abstract":"<div><div>Nowadays, producing an innovative platform for a controlled drug delivery system using agroindustry waste is a great opportunity for the sustainable, economic, and developed world. Thus, this research produced innovative nanocomposites based on the production of phosphate nanoparticles by the co-precipitation method on carboxymethyl cellulose (CMC) using eggshell and incorporating ketoconazole to combat <em>Candida albicans</em> in odontology treatments. Then, Raman, XRD, and SEM analyses were used to characterize these nanocomposites. Additionally, the kinetics of drug release were studied, including antimicrobial activity and cell viability. The results showed that effective nanocomposites with hydroxyapatite (HA) nanoparticles incorporated with a size close to 20 nm, modified by ketoconazole, were produced. Moreover, the ketoconazole does not affect the morphological and crystalline structure of the nanocomposite, and the CMC enhances its antimicrobial activity. Furthermore, all samples presented biocompatibility, as no cytotoxicity was visualized (≥85 %). Thus, these results showed that these nanocomposites possess a high potential for application in endodontic and periodontic treatment in odontology applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148157"},"PeriodicalIF":8.5,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tannic acid–enhanced complex coacervate microcapsules based on whey protein isolate and hyaluronic acid for the protection of Lactobacillus paracasei and their application in goat milk yogurt 基于分离乳清蛋白和透明质酸的单宁酸增强复合凝聚微胶囊对副干酪乳杆菌的保护及其在羊乳酸奶中的应用

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148151

Yuanye Liu, Hongcai Li, Mengxin Hou, Zhenpeng Gao

{"title":"Tannic acid–enhanced complex coacervate microcapsules based on whey protein isolate and hyaluronic acid for the protection of Lactobacillus paracasei and their application in goat milk yogurt","authors":"Yuanye Liu, Hongcai Li, Mengxin Hou, Zhenpeng Gao","doi":"10.1016/j.ijbiomac.2025.148151","DOIUrl":"10.1016/j.ijbiomac.2025.148151","url":null,"abstract":"<div><div>Probiotic microcapsules often encounter adverse conditions during processing and storage. This study improved the performance of <em>Lactobacillus paracasei</em> (LP) microcapsules by incorporating tannic acid (TA) into a whey protein isolate (WPI)–hyaluronic acid (HA) complex coacervation system. The optimal conditions were a WPI-to-HA ratio of 10:1, pH 3.6, and 0.06 % TA relative to WPI. Coacervate formation was driven by electrostatic interactions, while TA further enhanced hydrogen bonding and hydrophobic interactions, resulting in a denser network. Scanning electron microscopy confirmed the most compact structure for the WPI-TA-HA complex. The resulting WPI-TA-HA-L microcapsules achieved the highest encapsulation efficiency (76.36 %) and freeze-drying survival (93.2 %), with suitable moisture content (2.87 %) and hygroscopicity (12.44 %), thereby improving storage stability. Compared with free LP, WPI-TA-HA-L showed markedly higher tolerance to adverse conditions: viability remained at 8.34 log CFU/mL in 0.5 % bile salts and 5.43 log CFU/mL after heating at 80 °C for 300 s, and differential scanning calorimetry showed the highest melting temperature (119.93 °C), confirming superior thermal stability. In vitro digestion further demonstrated enhanced acid resistance. When applied to goat milk yogurt, WPI-TA-HA-L maintained probiotic viability, improved gel properties, reduced syneresis and post-acidification, and enhanced sensory acceptance, highlighting its potential for functional dairy applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148151"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carboxymethyl cellulose embedded silica membrane impregnated with ZVI NPs for the catalytic detoxification of methyl orange dye and its kinetic and optimization studies 羧甲基纤维素包埋二氧化硅膜浸渍ZVI NPs催化脱毒甲基橙染料及其动力学与优化研究

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148162

Shahkar Usama , Abdur Rauf , Zubair Ahmad , Imran Ullah , Cai-Hong Zhan , Taghrid S. AlOmar , Najla Almasoud , Amir Saeed , Arshad Iqbal

{"title":"Carboxymethyl cellulose embedded silica membrane impregnated with ZVI NPs for the catalytic detoxification of methyl orange dye and its kinetic and optimization studies","authors":"Shahkar Usama , Abdur Rauf , Zubair Ahmad , Imran Ullah , Cai-Hong Zhan , Taghrid S. AlOmar , Najla Almasoud , Amir Saeed , Arshad Iqbal","doi":"10.1016/j.ijbiomac.2025.148162","DOIUrl":"10.1016/j.ijbiomac.2025.148162","url":null,"abstract":"<div><div>Polymer composites, carboxymethyl cellulose (CMC) and silica-coated carboxymethyl cellulose (CMCSi) were used as supports for zero-valent iron nanoparticles (Fe<sup>0</sup> NPs), yielding dip-catalysts represented as CMC@Fe<sup>0</sup> and CMCSi@Fe<sup>0</sup>. The catalysts were created through the adsorption of Fe<sup>3+</sup> ions on the polymer matrices, with the subsequent chemical reduction with NaBH<sub>4</sub> in aqueous solution. Using FTIR, XRD, and FESEM, structural and morphological topographies were characterized. The composite's catalytic efficiency was evaluated through the degradation of methyl orange (MO) dye. CMCSi@Fe<sup>0</sup> and CMC@Fe<sup>0</sup> achieved 89.4 % and 85.0 % degradation in 8 and 13 min. Kinetic modeling disclosed that CMCSi@Fe<sup>0</sup> followed pseudo-first-order kinetics (R<sup>2</sup> = 0.921, <em>k</em><sub>app</sub> = 4.44 min<sup>−1</sup>), where CMC@Fe<sup>0</sup> best fitted a zero-order (R<sup>2</sup> = 0.917). The impact of reaction parameters, including temperature, NaBH<sub>4</sub> concentration, MO concentration, and pH, was thoroughly explored. At elevated temperature, acidic pH, and higher NaBH<sub>4</sub> dosage, optimal degradation occurred. These findings validate that CMC@Fe<sup>0</sup> and CMCSi@Fe<sup>0</sup> membranes not only act as effective catalysts for dye detoxification but also hold the potential for water filtration and functional group transformation applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148162"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, application and regeneration of chitosan composite adsorbents for arsenic removal: A review from a sustainable perspective 壳聚糖复合除砷吸附剂的制备、应用及再生研究进展

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148156

Huiping Zeng , Guoyang Ma , Xiaoying Zheng , Da Lin , Jie Zhang , Dong Li

{"title":"Preparation, application and regeneration of chitosan composite adsorbents for arsenic removal: A review from a sustainable perspective","authors":"Huiping Zeng , Guoyang Ma , Xiaoying Zheng , Da Lin , Jie Zhang , Dong Li","doi":"10.1016/j.ijbiomac.2025.148156","DOIUrl":"10.1016/j.ijbiomac.2025.148156","url":null,"abstract":"<div><div>Chitosan-based composite adsorbents have emerged as critical material for arsenic removal due to their cost-effectiveness and high-efficiency adsorption capabilities for heavy metals/pollutants. This review systematically addresses sustainable strategies for arsenic purification using chitosan composites. Synthesis strategies utilize industrial waste such as shrimp/crab shells and water treatment residues as raw materials, with a critical analysis of preparation methods including homogeneous synthesis and cross-linking polymerization to reduce reliance on traditional chemicals and achieve waste valorization. The adsorption mechanisms of chitosan composites with iron/aluminum and their oxides, such as electrostatic interactions and inner-sphere complexation, are elucidated, highlighting the synergistic effects in efficiently capturing arsenic. Post-adsorption separation and regeneration technologies, particularly alkaline desorption and magnetic separation, are reviewed to enable multiple adsorption cycles, while disposal solutions for end-of-life saturated adsorbents are proposed. Innovatively, a life cycle assessment (LCA) is introduced to evaluate the environmental impacts across the adsorbent's lifecycle, from raw material acquisition to end-of-life disposal, validating the sustainability of the “waste-to-resource” strategy. Future directions explore synergistic composite systems combining chitosan with advanced materials and intelligent platforms integrating point-of-zero-charge (pHpzc) with machine learning. This review provides a comprehensive, sustainability-oriented perspective, offering critical insights to advance chitosan-based adsorbents as sustainable, high-performance solutions for arsenic remediation challenges.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148156"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiscale analysis of mechanical and structural properties of agarose–silk fibroin hydrogels 琼脂糖-丝素水凝胶力学和结构特性的多尺度分析。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148133

Tomas Plichta , Katerina Mrazova , Veronika Richterova , Marketa Khyrova , Jaroslav Lukes , Josef Sepitka

{"title":"Multiscale analysis of mechanical and structural properties of agarose–silk fibroin hydrogels","authors":"Tomas Plichta , Katerina Mrazova , Veronika Richterova , Marketa Khyrova , Jaroslav Lukes , Josef Sepitka","doi":"10.1016/j.ijbiomac.2025.148133","DOIUrl":"10.1016/j.ijbiomac.2025.148133","url":null,"abstract":"<div><div>This study provides a comprehensive characterization of the agarose–silk fibroin hydrogels, using atomic force microscopy and scanning transmission electron microscopy to analyse their structure and assess the effect of composition on mechanical properties via nanoindentation and rheological analysis. These measurements enabled determination of mechanical properties, including the elastic and viscoelastic moduli at both the micro- and macroscale. The hydrogels exhibited a wide range of moduli depending on different degrees of network crosslinking, influenced by varying concentrations of agarose (1 or 2 wt%) and the percentage of fibroin fibres (0–4.5 wt%) as an interpenetrating component. The viscoelastic modulus (<em>G</em>') and the elastic modulus determined using a relaxation model (<em>E</em>), were 5–57 kPa and 1.2–110 kPa, respectively. The adhesion energy of these hydrogels was determined from nanoindentation curves and analysed using the JKR model, with values ranging from 0.031 to 0.066 J m<sup>−2</sup>. These results provide insight into how the hydrogels' microstructure influences their mechanical and transport properties. Incorporating fibroin into these gels modifies biological and biochemical characteristics of the gels, suggesting that such composite hydrogels could be further explored for potential applications in controlled release systems, extracellular matrix models, or tissue engineering scaffolds.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148133"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of starch/pectin packaging films adding cedarwood essential oil and application in giant tiger prawn preservation 香柏木精油淀粉/果胶包装薄膜的制备及其在大虎对虾保鲜中的应用。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148132

Ende Song , Zhongbing Tan , Yifei Chen , Bing Zhang , Qi Liu , Junling Leng , Xinyu Shen

{"title":"Preparation of starch/pectin packaging films adding cedarwood essential oil and application in giant tiger prawn preservation","authors":"Ende Song , Zhongbing Tan , Yifei Chen , Bing Zhang , Qi Liu , Junling Leng , Xinyu Shen","doi":"10.1016/j.ijbiomac.2025.148132","DOIUrl":"10.1016/j.ijbiomac.2025.148132","url":null,"abstract":"<div><div>Shrimp preservation currently relies mainly on petroleum-based plastic films and refrigeration, which, although effective, may release microplastics into the environment. Developing biodegradable alternatives is therefore of great significance. In this study, potato starch (PS)/apple pectin (AP)/cedarwood essential oil (CEO) composite films were prepared via the casting method and evaluated for their potential in giant tiger prawn preservation. CEO incorporation markedly improved the film's properties, including mechanical strength (from 9.6 % to 32.4 %), UV shielding efficiency (EAB from 7.47 % to 33.2 % at 600 nm), and antibacterial activity. Films containing 2 % CEO exhibited superior antibacterial performance. Preservation tests further demonstrated that 2 % CEO films effectively inhibited increases in total volatile basic nitrogen (TVB-N) and pH: after 48 h, TVB-N rise decreased from 42.55 % to 23.04 %, while pH increase was reduced from 0.83 to 0.16. These findings highlight the potential of PS/AP/CEO films as sustainable packaging materials for extending the shelf life of seafood.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148132"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

14-3-3ζ protein prevents formation of GSK3β-phosphorylated Tau protein fibrils. 14-3-3ζ蛋白阻止gsk3 β磷酸化Tau蛋白原纤维的形成。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148222

Gytis Kučinskas, Aneta Kozeleková, Kateřina Králová, Viliam Volko, Ondrej Šedo, Jozef Hritz

{"title":"14-3-3ζ protein prevents formation of GSK3β-phosphorylated Tau protein fibrils.","authors":"Gytis Kučinskas, Aneta Kozeleková, Kateřina Králová, Viliam Volko, Ondrej Šedo, Jozef Hritz","doi":"10.1016/j.ijbiomac.2025.148222","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148222","url":null,"abstract":"<p><p>Alzheimer's Disease remains one of the challenges in modern-day medicine, lacking any blockbuster therapy. Despite understanding the pathology and identifying neurofibrillary tangles as a hallmark of the disease, the molecular mechanisms behind it still remain unclear. Understanding post-translational modifications (PTMs) and 14-3-3 protein role can be crucial in uncovering tauopathies progression. Our study was focused on the phosphorylation of recombinant full-length Tau by Glycogen synthase kinase-3 beta (GSK3β). We targeted phosphorylation, particularly at the C terminus and residues identified as AD phospho-biomarkers. Additionally, our study investigated the effect of 14-3-3ζ (highly abundant in the human brain) on the fibrillisation of Tau phosphorylated by GSK3β. Using our optimised fibrillisation conditions, we compared Tau fibril formation both in the presence and absence of 14-3-3ζ protein. Notably, 14-3-3ζ protein significantly inhibited fibril formation under these conditions. This conclusion was supported by both quantitative measurements using the Thioflavin T (ThT) assay and qualitative assessments through visualisation techniques, including negative-stain electron microscopy (NS-EM) and atomic force microscopy (AFM). Besides, chemical cross-linking and nuclear magnetic resonance spectroscopy (NMR) revealed direct interaction between 14 and 3-3ζ and GSK3β-phosphorylated Tau, proposing the molecular mechanism of inhibition. These findings suggest that 14-3-3ζ may exert a protective role in Alzheimer's Disease by modulating Tau aggregation.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148222"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ophthalmic antioxidant activity of a model antianginal drug using corneal hydrogel formulation: Effect of carboxymethyl starch on physicomechanical behaviour and release/permeation kinetics. 角膜水凝胶模型抗心绞痛药物的眼抗氧化活性：羧甲基淀粉对物理力学行为和释放/渗透动力学的影响

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148219

Rakesh Swain, Sk Habibullah, Mouli Das, Tanisha Das, Arijit Prasad Meher, Subrata Mallick

{"title":"Ophthalmic antioxidant activity of a model antianginal drug using corneal hydrogel formulation: Effect of carboxymethyl starch on physicomechanical behaviour and release/permeation kinetics.","authors":"Rakesh Swain, Sk Habibullah, Mouli Das, Tanisha Das, Arijit Prasad Meher, Subrata Mallick","doi":"10.1016/j.ijbiomac.2025.148219","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148219","url":null,"abstract":"<p><p>Trimetazidine (TIZ), a model antianginal drug helps reducing the formation of free radicals. Carboxymethyl starch (CMS) is acknowledged to improve mucoadhesion, flexibility and strength in combination with hydroxypropyl methylcellulose (HPMC) compared to HPMC alone. Ocular antioxidant activity of trimetazidine hydrogel has been undertaken after topical application. Effect of CMS on physicomechanical performance and release/permeation kinetics was also studied. HPMC based hydrogel was prepared in different combination with CMS. Carrageenan induced rat eyeball was extracted for histological analysis and malondialdehyde assay. Presence of CMS significantly increased viscosity, and also improved hardness, adhesive force, adhesiveness, and cohesiveness compared to its absence of the hydrogel. Carboxymethyl group of the CMS increased hydrophilicity, hydration and swelling property of the hydrogel. TIZ hydrogel presented organized and prolonged diffusion controlled drug release through the cornea for around 9 h. The histology of rat eye revealed that, the ganglionic layer damage in retina has been reduced prominently in the TIZ hydrogel treated group. Flexible docking studies demonstrated a stronger binding interaction between TIZ and IL-1β (-5.4 kJ/mol) compared to diclofenac (-4.68 kJ/mol) and dexamethasone (-5.33 kJ/mol). Trimetazidine hydrogel could be used for controlled ophthalmic antioxidant activity after topical application. Presence of CMS improved physicomechanical performance.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148219"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0