International Journal of Biological Macromolecules最新文献

Green synthesis of hawthorn polysaccharide-stabilized Pd@Ru nanozymes for sensitive colorimetric detection of organophosphorus pesticides 绿色合成山楂多糖稳定Pd@Ru纳米酶用于有机磷农药的灵敏比色检测

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147780

Jiaoyang Wang , Qingtao Zeng , Kaile Cao , Yanshuai Cui , Haiyan Xiao , Longgang Wang

{"title":"Green synthesis of hawthorn polysaccharide-stabilized Pd@Ru nanozymes for sensitive colorimetric detection of organophosphorus pesticides","authors":"Jiaoyang Wang , Qingtao Zeng , Kaile Cao , Yanshuai Cui , Haiyan Xiao , Longgang Wang","doi":"10.1016/j.ijbiomac.2025.147780","DOIUrl":"10.1016/j.ijbiomac.2025.147780","url":null,"abstract":"<div><div>Organophosphorus pesticides (OPs) pose a significant threat to food safety due to their high toxicity and tendency to bioaccumulate. Conventional detection methods are constrained by reliance on specialized equipment and complex procedures, hindering rapid, on-site screening. In this study, a green synthesis approach was employed using hawthorn polysaccharide (HP) as a natural stabilizer to fabricate bimetallic Pd@Ru nanozymes (HP-Pd<sub>m</sub>Ru<sub>n</sub> NPs). The synergistic effect between palladium and ruthenium markedly enhanced the nanozymes' peroxidase-like catalytic activity and stability. Their catalytic behavior followed typical Michaelis–Menten kinetics, exhibiting a strong affinity for the substrate 3,3′,5,5′-tetramethylbenzidine (TMB). A colorimetric biosensor based on HP-Pd<sub>10</sub>Ru<sub>20</sub> NPs enabled rapid and sensitive detection of glyphosate, with a detection limit as low as 0.179 ng/mL and a linear range of 0.0002–200 μg/mL. The biosensor demonstrated excellent selectivity and recovery, offering promising potential for bioanalytical applications and contributing to the development of environmentally friendly, cost-effective technologies for detecting OPs residues.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"329 ","pages":"Article 147780"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanism of heavy rare earth elements-induced G-quadruplex DNA: Structural switch in human telomeric variants. 重稀土元素诱导g -四重体DNA的分子机制：人类端粒变异的结构开关。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147817

Sampat N Satapathy, Umakanta Subudhi

{"title":"Molecular mechanism of heavy rare earth elements-induced G-quadruplex DNA: Structural switch in human telomeric variants.","authors":"Sampat N Satapathy, Umakanta Subudhi","doi":"10.1016/j.ijbiomac.2025.147817","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.147817","url":null,"abstract":"<p><p>Heavy rare earth elements (HREEs) are vital to modern technologies, yet their interactions with nucleic acids remain underexplored to date. With rising environmental exposure, their potential to modulate telomeric DNA structures raises concerns about genomic stability. In this study, we demonstrate that micromolar concentrations of HREEs induce stable G-quadruplexes (GQs) in human telomeric DNA and its variants. Conformational study revealed the formation of antiparallel GQs across all telomeric variants; thermal analysis further confirmed sequence-dependent differences in GQ stability, with thymine-rich loops exhibiting enhanced structural stability. To validate GQ formation, <sup>1</sup>H NMR spectroscopy was employed, providing direct evidence of Hoogsteen hydrogen bonding. Binding affinity and stoichiometry were determined through thermodynamic characterization, revealing a 2:1 HREE-DNA stoichiometry and favorable binding energetics. Vibrational spectroscopic analysis further revealed characteristic shifts associated with guanine ring and phosphate backbone interactions. The impact of metal coordination on structural charge distribution was evaluated through zeta potential analysis, which revealed significant electrostatic shifts upon GQ formation. Finally, Competitive binding studies demonstrated that HREEs displace Na<sup>+</sup>/K<sup>+</sup> ions, resulting in conformational transitions. Collectively, these findings provide detailed insight into HREE-induced GQ formation, with implications for telomere biology, environmental toxicity, and nucleic acid-based biosensing.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"147817"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and preliminary anti-inflammatory activity of a novel sulfated α-glucan from Volutharpa ampullacea perryi. 一种新型磺化α-葡聚糖的结构及初步抗炎活性研究。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147806

Yiling Chen, Yong Qin, Haixin Yi, Xiaoyu Cui, Xu Wang, Xiangyu Xu, Yongmei Chen, Fuchuan Li, Wenshuang Wang

{"title":"Structure and preliminary anti-inflammatory activity of a novel sulfated α-glucan from Volutharpa ampullacea perryi.","authors":"Yiling Chen, Yong Qin, Haixin Yi, Xiaoyu Cui, Xu Wang, Xiangyu Xu, Yongmei Chen, Fuchuan Li, Wenshuang Wang","doi":"10.1016/j.ijbiomac.2025.147806","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.147806","url":null,"abstract":"<p><p>Glucans, as versatile biomacromolecules, hold significant application potential across various industries. Volutharpa ampullacea perryi, an affordable deep-sea gastropod, demonstrates considerable commercial promise. In this study, a novel sulfated α-glucan (VpG) was isolated and identified from Volutharpa ampullacea perryi for the first time. Compared to other α-sulfated glucans, it has a high degree of sulfation (18.6 %) and an ultra-high molecular weight of approximately 2250 kDa. Further NMR analysis, including <sup>1</sup>H, <sup>13</sup>C, COSY, TOCSY, HSQC and HMBC, revealed that the anomeric signals at 5.35 ppm dominated. Therefore, VpG is mainly consisted of a repeating α-(1 → 4)-linked glucose backbone with β-(1 → 6) and β-(1 → 3)-linked glucosyl branches. Furthermore, bioactivity evaluation demonstrated that VpG significantly alleviates inflammation in dextran sulfate sodium-induced mouse models of ulcerative colitis, effectively inhibiting body weight loss by 20 % and colon shortening by 31.26 %. The strong anti-inflammatory activity of VpG indicates its potential in pharmaceutical development and provides a novel approach for the high-value utilization of Volutharpa ampullacea perryi.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"147806"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and virtual screening of novel umami peptides from dry-cured ham bone gelatin through molecular docking 基于分子对接的干腌火腿骨明胶新型鲜味肽的鉴定与虚拟筛选

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147802

Ming Ju , Zixu Wang , Lujuan Xing , Chunxiang Piao , Guanhao Li , Wangang Zhang

{"title":"Identification and virtual screening of novel umami peptides from dry-cured ham bone gelatin through molecular docking","authors":"Ming Ju , Zixu Wang , Lujuan Xing , Chunxiang Piao , Guanhao Li , Wangang Zhang","doi":"10.1016/j.ijbiomac.2025.147802","DOIUrl":"10.1016/j.ijbiomac.2025.147802","url":null,"abstract":"<div><div>This study aimed to identify novel umami peptides (UPs) from dry-cured ham bone gelatin (DHBG) and elucidated their umami mechanism. Gelatin was extracted from dry-cured ham bones using citric acid combined with pepsin with the yield of 13.52 %. Subsequently, DHBG was hydrolyzed using alkaline protease and flavorzyme. Through a combination of peptidomics and virtual screening techniques, two novel umami peptides, YDGE and EVGAP, were identified from the hydrolysate, with binding energies of −7.862 and − 7.861 kcal/mol, respectively. Molecular docking analysis indicated that both peptides could bind to the active site within the T1R3 subunit of the umami taste receptor, with hydrogen bonds as the predominant interaction forces. qPCR analysis showed that both peptides significantly upregulated (<em>P</em> < 0.05) mRNA expression of T1R1, T1R3, and their downstream effector PLCβ2 in STC-1 cells. Additionally, at a concentration range of 0.25 to 2 mg/mL, both synthetic peptides, similar to MSG, effectively induced increased intracellular Ca<sup>2+</sup> in STC-1 cells. These findings highlight the potential application of DHBG as a flavoring agent and offer perspectives on the mechanism of interaction between receptors and UPs.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"329 ","pages":"Article 147802"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-catalytic preparation of poly(methacrylic acid) hydrogels using peroxidase from potato peel waste and preliminary evaluation of their anti-inflammatory potential. 马铃薯皮废过氧化物酶生物催化制备聚甲基丙烯酸水凝胶及其抗炎活性初步评价。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147762

Maja D Markovic, Vukasin Dj Ugrinovic, Huan Meng, Zhen Li, Sibylle Grad, Milica M Svetozarevic Arsovic

{"title":"Bio-catalytic preparation of poly(methacrylic acid) hydrogels using peroxidase from potato peel waste and preliminary evaluation of their anti-inflammatory potential.","authors":"Maja D Markovic, Vukasin Dj Ugrinovic, Huan Meng, Zhen Li, Sibylle Grad, Milica M Svetozarevic Arsovic","doi":"10.1016/j.ijbiomac.2025.147762","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.147762","url":null,"abstract":"<p><p>A new eco-friendly initiator based on potato peel peroxidase, vitamin C and H<sub>2</sub>O<sub>2</sub> (PP/VC/H<sub>2</sub>O<sub>2</sub>) is used for free radical polymerization of methacrylic acid. Fifty million tons of global potato peel waste is generated annually, so isolation of active substances, such as peroxidase is a favorable solution from economic and environmental aspects. By employing the PP/VC/H<sub>2</sub>O<sub>2</sub> initiator, pH sensitive hydrogels based on poly(methacrylic acid) (PMAA) can be successfully prepared by simple, energy efficient and sustainable synthesis under ambient conditions. The reaction time and the required amount of H₂O₂ are significantly reduced (to 8 h and 30 μL, respectively) compared to the method employing H₂O₂/VC initiation system, in which reaction is completed after 24 h and requires 100 μL of H<sub>2</sub>O<sub>2</sub>. Various techniques are used for characterization of the PMAA hydrogels: DSC, XRD, FTIR, SEM and single compression tests. PP is analyzed, and its optimal pH and temperature, specific activity, and RZ factor, are determined. Considering that inflammation can cause damage to the human body and lead to many serious diseases (such as osteoarthritis), anti-inflammatory drug - dexamethasone is encapsulated in the PMAA hydrogels. Controlled release of dexamethasone lasts 24 h. The PMAA swelling properties and dexamethasone release are investigated depending on various synthesis parameters in media with different pH values. The anti-inflammatory effect of the PMAA hydrogel with dexamethasone is tested on bovine chondrocytes. Results show that PMAA hydrogels have anti-inflammatory effect with properties achieved through mild, eco-friendly, economical, and tunable synthesis conditions.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"147762"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface functionalization with short PAS-sequence affects H-ferritin nanocage stability. 短pas序列的表面功能化影响h -铁蛋白纳米笼的稳定性。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147809

Ilaria Tagliolini, Francesca Gorgoglione, Marta Sevieri, Beatrice Bignami, Valeria Giacobbo, Claudia Pigliacelli, Francesca Baldelli Bombelli, Fabio Corsi, Renata Tisi, Serena Mazzucchelli

{"title":"Surface functionalization with short PAS-sequence affects H-ferritin nanocage stability.","authors":"Ilaria Tagliolini, Francesca Gorgoglione, Marta Sevieri, Beatrice Bignami, Valeria Giacobbo, Claudia Pigliacelli, Francesca Baldelli Bombelli, Fabio Corsi, Renata Tisi, Serena Mazzucchelli","doi":"10.1016/j.ijbiomac.2025.147809","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.147809","url":null,"abstract":"<p><p>H-ferritin nanocages (HFn) are promising tumor-targeting delivery systems owing to their specificity and reduced off-target toxicity. However, their short half-life and rapid clearance currently limit clinical application. PASylation has been used to increase the in vivo half-life of HFn, without introducing negative effects. To date, all knowledge is limited to PASylated HFn containing PAS domains of 40 and 75 amino acids. Herein, we investigate the impact of shorter PAS domains on the stability of HFn, focusing on a mutant modified with 20 amino acids PAS domains. PAS20-HFn monomers were successfully produced and purified but seems unable to form stable quaternary structure. To investigate this, we studied the structural dynamics properties of native HFn, PAS20-HFn, PAS40-HFn dimers, tetramers, and octamers. Our simulations indicate that PAS20-HFn tetramers exhibit increased flexibility in a region encompassing the end of the D helix, the D-E turn and the beginning of the E helix. This flexibility is critical for dimer interactions and facilitates the subsequent organization of higher-order complexes. During the folding of PAS20-HFn octamers, PAS domain disrupts the proper association of the C-terminal E-helices, leading to instability in the nanocages. This highlights the bipolar and essential role of HFn PASylation in HFn nanocages, especially concerning their size.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"147809"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The glycoside hydrolase 61 member PgGH61-2 of Pyrenophora graminea is required for full virulence on barley (Hordeum vulgare). 稻瘟病菌的糖苷水解酶61成员PgGH61-2对大麦（Hordeum vulgare）具有完全毒力。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147577

Ming Guo, Erjing Si, Yanxiu Wang, Juncheng Wang, Lirong Yao, Yaxiong Meng, Xiaole Ma, Baochun Li, Hong Zhang, Ke Yang, Xunwu Shang, Huajun Wang

{"title":"The glycoside hydrolase 61 member PgGH61-2 of Pyrenophora graminea is required for full virulence on barley (Hordeum vulgare).","authors":"Ming Guo, Erjing Si, Yanxiu Wang, Juncheng Wang, Lirong Yao, Yaxiong Meng, Xiaole Ma, Baochun Li, Hong Zhang, Ke Yang, Xunwu Shang, Huajun Wang","doi":"10.1016/j.ijbiomac.2025.147577","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.147577","url":null,"abstract":"<p><p>Barley leaf stripe caused by Pyrenophora graminea is a main barley (Hordeum vulgare L.) disease worldwide. However, the effectors secreted by P. graminea molecular mechanisms are largely unknown. The members of the glycoside hydrolase (GH) family serve as virulence factors and modulate plant immune responses during pathogen infection. In this study, we identified a GH 61 family member lytic polysaccharide monooxygenase PgGH61-2, secreted by P. graminea. The expression of PgGH61-2 was highly upregulated during the early stages of P. graminea infection of barley. PgGH61-2 was acted as a pathogen-associated molecular pattern (PAMP) to cause BAX-induced cell death in Nicotiana benthamiana. The growth rate of PgGH61-2 interfering and overexpressing strains were reduced and increased significantly compared with the wild-type strain, respectively. Furthermore, silencing PgGH61-2 significantly reduced P. graminea pathogenicity in barley, whereas its overexpression increased disease severity. Additionally, these interfering strains displayed diminished ability to exploit carbon sources, rendering them more susceptible to osmotic and cell wall stresses. Therefore, our results suggest that PgGH61-2 is an important pathogenicity factor and can induce plant defense responses.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"147577"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization and biological activities of sulfated polysaccharides from Antrodia cinnamomea 肉桂酸酸化多糖的结构表征及生物活性研究

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147801

Chia-I Jen , Wei-Lun Qiu , Chi-Hsein Chao , Mei-Kuang Lu

{"title":"Structural characterization and biological activities of sulfated polysaccharides from Antrodia cinnamomea","authors":"Chia-I Jen , Wei-Lun Qiu , Chi-Hsein Chao , Mei-Kuang Lu","doi":"10.1016/j.ijbiomac.2025.147801","DOIUrl":"10.1016/j.ijbiomac.2025.147801","url":null,"abstract":"<div><div><em>Antrodia cinnamomea</em>, an edible and medicinal mushroom, has been widely used for functional foods and dietary supplements in Taiwan. This study discovered β-1,6-linked sulfated galactoglucans with or without branches from <em>A. cinnamomea</em> mycelia, and elucidated their anti-inflammatory and anti-cancer potentials. Results showed that K1, a sulfated polysaccharide (SPS) isolated from <em>A. cinnamomea</em> mycelia cultured for 49 days with 1 mM potassium sulfate, possessed potential anti-inflammation in that K1 100 μg/ml suppressed tumor necrosis factor-α (TNF-α) production by 49%, and anti-cancer activity with K1 800 μg/ml suppressed lung cancer H1975 cells by 57%. Based on the molecular weight distribution, three SPS fractions (K1 F1, K1 F2, and K1 F3) were purified from K1. Among the three fractions, K1 F2 and K1 F3 were found to contain high sulfate content with values of 4.46 and 3.77 mmol/g, respectively. The representative repeating unit of K1 F2 contained a sulfated β-1,6-linked glucosyl backbone with a long α-/β-galactoglucose branch attached at the 3-<em>O</em> position of backbone, while the proposed repeating unit of K1 F3 included an unbranched sulfated β-1,6-linked glucosyl residue. K1 F2 and K1 F3 attenuated the LPS-induced inflammation in RAW264.7 cells via p38 and AKT pathways, respectively. K1 F2 possessed potential anti-cancer activity against H1975 lung cancer cells through TGFβRI and apoptotic pathways. In conclusion, K1 F2, a long-branched sulfated galactoglucan, and K1 F3, an unbranched sulfated glucan, may be good candidates for anti-inflammatory and anti-cancer applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"329 ","pages":"Article 147801"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cannabis polysaccharides (CPs): Extraction methods, structural characteristics, biological activities and structure-function relationship: A review 大麻多糖（CPs）：提取方法、结构特征、生物活性及结构功能关系综述

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147770

Adil Hussain , Syed Ali Imran Bokhari

{"title":"Cannabis polysaccharides (CPs): Extraction methods, structural characteristics, biological activities and structure-function relationship: A review","authors":"Adil Hussain , Syed Ali Imran Bokhari","doi":"10.1016/j.ijbiomac.2025.147770","DOIUrl":"10.1016/j.ijbiomac.2025.147770","url":null,"abstract":"<div><div><em>Cannabis</em> polysaccharides (CPs) have attracted growing interest due to their diverse structures and potential bioactivities. This review consolidated current knowledge on the CPs extraction, their characterization, molecular properties and biofunctional potentials. Composition of the CPs varied by plant part, cultivar and origin with most studies focused on seed derived polysaccharides. Extraction methods, particularly the hot water and enzyme assisted extractions differ in temperature, duration and solid–liquid ratio influencing the final yield and molecular integrity. Advanced analyses such as HPAEC-PAD, HPSEC, GC–MS and FT–IR revealed monosaccharides such as mannose, glucose, galactose, glucuronic acid and galacturonic acid, along with proteins, sulfates and uronic acids. The reported molecular weight range was 4.21 to 864.17 kDa representing both low and high Mw fractions. The CPs were reported with antioxidant and antiaging activities which are often mediated via the Nrf2/Keap1–ARE pathway. These activities are shaped by the Mw and sugar composition with low-Mw fractions having enhanced antioxidant activity. Rare sugars and sulfated residues further enhanced the bioactivity suggesting specialized therapeutic applications. Emerging evidences also pointed to the roots, leaves and hemp residues as underexplored sources with potential for novel bioactivities. This review underscores the structural diversity of CPs, the effect of extraction methods on yield and biofunctional properties, their key bioactivities and the underlying mechanisms. Collectively, these insights provide a comprehensive foundation and research roadmap for advancing the CPs in pharmaceutical, nutraceutical and functional food applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"329 ","pages":"Article 147770"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MXene/cellulose conductive hydrogel with stretchability, anti-swelling, antibacteriality and environmental adaptability for wearable flexible strain sensors in human behavior monitoring 具有拉伸性、抗膨胀性、抗菌性和环境适应性的MXene/纤维素导电水凝胶，用于人体行为监测中的可穿戴柔性应变传感器

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-09-19 DOI: 10.1016/j.ijbiomac.2025.147784

Kai Yang Li , Da Hai Yang , Changling Liu , Wei Jiang , Yan Liu , Hong Yu Yang , Bo Wang , Yan Fu

{"title":"MXene/cellulose conductive hydrogel with stretchability, anti-swelling, antibacteriality and environmental adaptability for wearable flexible strain sensors in human behavior monitoring","authors":"Kai Yang Li , Da Hai Yang , Changling Liu , Wei Jiang , Yan Liu , Hong Yu Yang , Bo Wang , Yan Fu","doi":"10.1016/j.ijbiomac.2025.147784","DOIUrl":"10.1016/j.ijbiomac.2025.147784","url":null,"abstract":"<div><div>The advancement of wearable flexible strain sensors has attracted considerable attention, given their potential to transformative impact human behavior monitoring and enhance human-computer interaction. This study synthesized from carboxymethyl cellulose (CMC) grafted with poly(acrylic acid-<em>co</em>-vinyl imidazole) P(AA-co-VI) and MXene nanosheets, to obtain a multifunctional hydrogel termed C-PAV/MXene. The hydrogel combines the exceptional ductility of CMC with a robust, crosslinked architecture formed by acrylic acid-derived dynamic hydrogen bonds, which enhances mechanical resilience while minimizing volumetric expansion. The inclusion of MXene nanosheets further providing superior electrical conductivity, efficient near-infrared (NIR)-driven photothermal conversion, and inherent antibacterial properties. The synthesized conductive hydrogels demonstrate impressive tensile properties, with an elongation of 964 %, and exhibit high linearity across the entire strain range (R<sup>2</sup> = 0.98). This hydrogel achieves antibacterial efficacy through a dual mechanism that combines quaternary ammonium groups with the physical bactericidal action of MXene. Furthermore, it effectively converts physical signals generated by human motion into corresponding electrical changes, maintaining efficacy even during motion monitoring in aquatic environments. Moreover, it facilitates the transmission of information through Morse code via variations in pressure and strain. This multifunctional hydrogel demonstrates transformative potential for next-generation wearable electronics by addressing critical limitations in strain/pressure sensing applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"329 ","pages":"Article 147784"},"PeriodicalIF":8.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0