International Journal of Biological Macromolecules最新文献

C-phycocyanin and quaternized chitosan based antibiotic-free hydrogels with antioxidant and antibacterial activity for wound healing.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-02-03 DOI: 10.1016/j.ijbiomac.2025.140647

Susu Zhang, Congcong Huang, Zengyu Sun, Penghua Jiao, Baoqi Ding, Luyao Ding, Qianfen Qi, Yanzhe Gou, Renlong Zhang, Yanhao He, Wenjun Li, Chunguang Ren, Linlin Wang

{"title":"C-phycocyanin and quaternized chitosan based antibiotic-free hydrogels with antioxidant and antibacterial activity for wound healing.","authors":"Susu Zhang, Congcong Huang, Zengyu Sun, Penghua Jiao, Baoqi Ding, Luyao Ding, Qianfen Qi, Yanzhe Gou, Renlong Zhang, Yanhao He, Wenjun Li, Chunguang Ren, Linlin Wang","doi":"10.1016/j.ijbiomac.2025.140647","DOIUrl":"10.1016/j.ijbiomac.2025.140647","url":null,"abstract":"Hydrogels with antioxidant and antibacterial activities have received increasing attention in wound healing due to excessive reactive oxygen species (ROS) and bacterial infection are common issues associated with wounds. Herein, we constructed a series of hydrogels with C-phycocyanin (C-PC), quaternized chitosan (QCS) and silk fibroin protein (SF) as matrixes, which with tetrakis hydroxymethyl phosphonium sulfate (THPS) as crosslinking agent to form dynamic covalent bonds with C-PC and SF. The hydrogel exhibited excellent stretchability and compressibility, which with adhesion strength reached 15 ± 3 kPa and rapid self-healing properties. The hydrogel possessed strong antioxidant activity with assessments of DPPH radical-scavenging capacity and total reducing power. In addition, the hydrogel possessed obvious coagulation function and good blood compatibility, which also showed strong antibacterial activity against E. coli and S. aureus. To improve the therapeutic effect, polydeoxyribonucleotide (PDRN) with the ability of promote wound healing was introduced into the hydrogel. The results showed that the hydrogel loading with PDRN possessed high biocompatibility and can promote cell migration. More importantly, the hydrogel loaded with PDRN can effectively promote wound healing by exerting anti-inflammatory and antioxidant effects, which may offer promising potential application value in the field of wound dressing and tissue repair.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140647"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143253986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Preparation and characterization of PVA/arginine chitosan/ZnO NPs composite films" [Int. J. Biol. Macromol. 226 (2023) 184-193].

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-02-09 DOI: 10.1016/j.ijbiomac.2025.140515

Liu Yang, Lixuan Ren, Yanzhen Zhao, Siqun Liu, Huifang Wang, Xianghua Gao, Baolong Niu, Wenfeng Li

引用次数: 0

Salidroside production through cascade biocatalysis with a thermostability-enhanced UDP-glycosyltransferase.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-23 DOI: 10.1016/j.ijbiomac.2025.140261

Guosi Li, Shanyong Yi, Haijiao Wang, Hulin Qiu, Wei Wang, Leilei Gao, Qilin Xu, Bangxing Han, Xinjian Yin

{"title":"Salidroside production through cascade biocatalysis with a thermostability-enhanced UDP-glycosyltransferase.","authors":"Guosi Li, Shanyong Yi, Haijiao Wang, Hulin Qiu, Wei Wang, Leilei Gao, Qilin Xu, Bangxing Han, Xinjian Yin","doi":"10.1016/j.ijbiomac.2025.140261","DOIUrl":"10.1016/j.ijbiomac.2025.140261","url":null,"abstract":"Salidroside is a phenylpropanoid glycoside with wide applications in the food, pharmaceutical, and cosmetic industries; however, the plant genus Rhodiola, the natural source of salidroside, has slow growth and limited distribution. In this study, we designed a novel six-enzyme biocatalytic cascade for the efficient production of salidroside, utilizing cost-effective bio-based L-Tyrosine as the starting material. A preliminary analysis revealed that the poor thermostability of the Bacillus licheniformis UDP-glycosyltransferase (EC 2.4.1.384) BlYjiC M6 is a bottleneck in the cascade. Therefore, a combined computational strategy was used to engineer it and finally obtained a mutant TSM6 (T304V/G307A/N309W/F123W/T344V/D271G) with a 134-fold longer half-life at 40 °C and a 13 °C higher Tmapp compared to M6. The integration of TSM6 into the cascade improved salidroside productivity significantly, while reducing residual intermediates. After further optimization, the whole-cell biocatalytic cascade achieved a high salidroside titer of 12.8 g·L-1 in a 5 L bioreactor, giving a productivity of 0.53 g·L-1·h-1. This study provides a green and efficient biosynthetic process for salidroside production and highlights the potential of enzyme engineering to enhance the biocatalytic cascade.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"299 ","pages":"140261"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional value improvement of soybean meal through solid-state fermentation by proteases-enhanced Streptomyces sp. SCUT-3. 蛋白酶增强链霉菌SCUT-3固态发酵提高豆粕营养价值

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-17 DOI: 10.1016/j.ijbiomac.2025.140035

De-Lin Lu, Ming-Shu Zhang, Fu-Bao Wang, Zhen-Jie Dai, Zhi-Wei Li, Jing-Tao Ni, Wen-Jing Feng, Fu-Gen Zhang, Jun Dai, Hai-Ning Wang, Jun-Jin Deng, Xiao-Chun Luo

{"title":"Nutritional value improvement of soybean meal through solid-state fermentation by proteases-enhanced Streptomyces sp. SCUT-3.","authors":"De-Lin Lu, Ming-Shu Zhang, Fu-Bao Wang, Zhen-Jie Dai, Zhi-Wei Li, Jing-Tao Ni, Wen-Jing Feng, Fu-Gen Zhang, Jun Dai, Hai-Ning Wang, Jun-Jin Deng, Xiao-Chun Luo","doi":"10.1016/j.ijbiomac.2025.140035","DOIUrl":"10.1016/j.ijbiomac.2025.140035","url":null,"abstract":"With the global population expected to reach 10 billion by the 2050s, the demand for protein will surge, intensifying the need for high protein utilization efficiency. This study investigates the use of protease-enhanced Streptomyces sp. SCUT-3-3940 to degrade soybean meal (SBM) via solid-state fermentation (SSF). Optimized conditions resulted in anti-nutritional factors elimination and high soluble protein recovery (41.1 g/100 g), including bioactive oligopeptides (17.3 g/100 g) with antihypertensive and antioxidant properties. The degradation also produced free amino acids rich in essential amino acids, and other nutrient enhancing compounds. The fermented SBM (FSBM) exhibited superior digestibility, making it a valuable protein source. In a 60-day largemouth bass trial, replacing 10 % SBM with FSBM in feed significantly improved feed intake and weight gain. This method offers an efficient, eco-friendly, and cost-effective solution to address global protein shortages.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140035"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting the high affinity between cellulose nanofibrils and Aloe vera acemannan to develop elastic, crosslinker-free, all-polysaccharide hydrogels.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-02-10 DOI: 10.1016/j.ijbiomac.2025.140853

Ngoc Huynh, Lukas Fliri, Juan José Valle-Delgado, Monika Österberg

{"title":"Exploiting the high affinity between cellulose nanofibrils and Aloe vera acemannan to develop elastic, crosslinker-free, all-polysaccharide hydrogels.","authors":"Ngoc Huynh, Lukas Fliri, Juan José Valle-Delgado, Monika Österberg","doi":"10.1016/j.ijbiomac.2025.140853","DOIUrl":"10.1016/j.ijbiomac.2025.140853","url":null,"abstract":"Plant-based polymers hold promising prospects thanks to their bioactivity, diversity and versatility but they are currently overshadowed by synthetic and animal-derived materials, especially in biomedical applications. In this study, we developed an entirely plant-based hydrogel with improved mechanical performance based on TEMPO-oxidized cellulose nanofibrils (TCNFs) and the polysaccharide fraction (AVPF) extracted from Aloe vera L. (Aloe barbadensis Miller). The hydrogel blends exhibited excellent viscoelastic properties, minimal shrinkage and a significant increase in compressive modulus (ranging from 2.7 to 13.2 kPa versus 0.8 kPa in single component hydrogels), suggesting a synergistic effect. In-depth analysis of interaction and morphology of the hydrogels by QCM-D, AFM and SEM imaging showed that the observed synergy was the result of the complementary action between the two components and a uniform spatial distribution of the two networks. TCNFs built the rigid skeleton for the hydrogels, while AVPF physically adsorbed on TCNFs, forming a flexible matrix, allowing for better load transfer and dissipation in both static and dynamic loading, leading to a remarkable increase in moduli that surpassed the mere sum of the two individual components. In addition, the obtained hydrogels also showed little to no perceptible shrinkage after drying, unlike the single-component hydrogels made from the initial materials. These hydrogels offer a sustainable and ethical alternative to animal-derived materials, with great potential in biomedical fields.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140853"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular structure of NRG-1 protein and its impact on adult hypertension and heart failure: A new clinical Indicator diagnosis based on advanced machine learning.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-02-11 DOI: 10.1016/j.ijbiomac.2025.140955

Qiyuan Bai, Hao Chen, Hongxu Liu, Xuhua Li, Yang Chen, Dan Guo, Bing Song, Cuntao Yu

引用次数: 0

Identification, characterization and molecular docking study of umami peptides from Spanish mackerel head enzymatic hydrolysate and Maillard reaction products.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-02-12 DOI: 10.1016/j.ijbiomac.2025.140876

Xuhua Tang, Shouyu Feng, Yanwei Liu, Wenhui Zhu, Ying Bu, Jianrong Li, Cikun Liu, Xuepeng Li

{"title":"Identification, characterization and molecular docking study of umami peptides from Spanish mackerel head enzymatic hydrolysate and Maillard reaction products.","authors":"Xuhua Tang, Shouyu Feng, Yanwei Liu, Wenhui Zhu, Ying Bu, Jianrong Li, Cikun Liu, Xuepeng Li","doi":"10.1016/j.ijbiomac.2025.140876","DOIUrl":"10.1016/j.ijbiomac.2025.140876","url":null,"abstract":"Umami peptides were screened and identified from the enzymatic hydrolysate of Spanish mackerel head and its Maillard reaction products using ultrafiltration, gel chromatography, and LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry). The umami properties of these peptides were subsequently evaluated and characterized using electronic tongue analysis and molecular docking. This study is the first to employ enzymatic hydrolysis combined with Maillard reaction for the preparation of umami peptides from Spanish mackerel head. Following this approach, a total of nine novel umami peptides were identified, including five from enzymatic hydrolysate (YDDKIY, ITPDEKGTTF, DAITTDDAGK, LEDGYPKEIQE, DAITPDEKGTTF) and four from Maillard reaction products (KDEGSDV, TPDEKGT, TEKAKGEP, FDAITPDEKGTTF). Sensory evaluation and electronic tongue analysis confirmed their distinct umami properties, with taste recognition thresholds ranging from 0.125 to 0.25 mg/mL. Molecular docking analysis revealed that these peptides interact with the T1R1/T1R3 umami receptor through hydrogen bonding and hydrophobic interactions, with key binding residues identified as Ser150, Ser256, and Glu128. This study provides a novel methodology for screening umami peptides from seafood by-products and lays the groundwork for their application as natural umami enhancers in the food industry.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140876"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyaluronic acid/ε-polylysine hydrogel enriched with Saussurea involucrata polysaccharide for improved skin dryness induced by ultraviolet radiation 富含莎草内卷多糖的透明质酸/ε-聚赖氨酸水凝胶可改善紫外线辐射引起的皮肤干燥问题

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 DOI: 10.1016/j.ijbiomac.2025.142718

Xiashun Xu , Xulong Huang , Shisheng Lin , Guangdong Yu , Jiali Xiao , Junchao Wang , Chengyang Song , Farid Khalilov , Nipatha Issaro , Nuo Xu , Shuang Gao

{"title":"Hyaluronic acid/ε-polylysine hydrogel enriched with Saussurea involucrata polysaccharide for improved skin dryness induced by ultraviolet radiation","authors":"Xiashun Xu , Xulong Huang , Shisheng Lin , Guangdong Yu , Jiali Xiao , Junchao Wang , Chengyang Song , Farid Khalilov , Nipatha Issaro , Nuo Xu , Shuang Gao","doi":"10.1016/j.ijbiomac.2025.142718","DOIUrl":"10.1016/j.ijbiomac.2025.142718","url":null,"abstract":"<div><div>Ultraviolet B (UVB) radiation exposure is a major contributor to skin photoaging, ultimately resulting in dryness. Conventional treatments for this condition frequently exhibit limited efficacy due to poor skin penetration and are associated with significant side effects. Saussurea involucrate polysaccharide (SIP) has been shown to alleviate photoaging-induced dry skin; however, its effectiveness is limited due to low drug utilization and the need for frequent dosing. In this study, we developed hyaluronic acid (HA) and ε-polylysine (ε-PLL) hydrogels designed to enhance skin hydration and enable sustained drug release. The optimal formulation, designated A5E3, contained 5 % HA plus 3 % ε-PLL. The sustained release of SIP from A5E3 demonstrated superior therapeutic effects in a mouse model of skin photoaging, including reduced inflammation and apoptosis, as well as enhanced keratinocyte differentiation and lipid production. The A5E3 hydrogel not only enhanced the drug utilization of SIP but also provided a sustained moisturizing environment, promoting faster skin repair. These findings support the development of skincare products with superior moisturizing and reparative properties, underscoring the potential of hydrogels as effective carriers for treating UVB-induced skin dryness and damage.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142718"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial oxidative stress related LncRNA predict cervical cancer prognosis and immunotherapy response: Molecular structure and protein interaction of ribosomal protein L34.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-20 DOI: 10.1016/j.ijbiomac.2025.140145

Nanchang Wu, Fengjuan Lin, Jianying Ji, Hongxia Pan, Lingyuan Xiang, Guanxiu Xian, Shengcai Chen

{"title":"Mitochondrial oxidative stress related LncRNA predict cervical cancer prognosis and immunotherapy response: Molecular structure and protein interaction of ribosomal protein L34.","authors":"Nanchang Wu, Fengjuan Lin, Jianying Ji, Hongxia Pan, Lingyuan Xiang, Guanxiu Xian, Shengcai Chen","doi":"10.1016/j.ijbiomac.2025.140145","DOIUrl":"10.1016/j.ijbiomac.2025.140145","url":null,"abstract":"The purpose of this study was to investigate the predictive value of mitochondrial oxidative stress-related LncRNA in cancer prognosis and immunotherapy response, and to further analyze the molecular structure of ribosomal protein L34 and its interaction mechanism with the protein. We screened lncrnas associated with mitochondrial oxidative stress, evaluated their expression patterns in different cancer types, and analyzed the three-dimensional structure of ribosomal protein L34 and its interaction network with other proteins. In this study, public databases were used to screen out lncrnas associated with mitochondrial oxidative stress. Bioinformatic analysis, including gene expression profile analysis, survival analysis and functional enrichment analysis, was used to evaluate the expression patterns of these lncrnas in different cancer types and their relationship with prognosis. The interacting proteins of ribosomal protein L34 were identified by proteomic techniques. The three-dimensional structure of ribosomal protein L34 and its binding mode with interacting proteins were studied by molecular docking and dynamic simulation methods. The results showed that the screened lncrnas showed significant expression differences in multiple cancer types and were closely related to the survival rate of patients. The three-dimensional structure of ribosomal protein L34 reveals key amino acid residues and binding sites for its interactions with specific proteins. Functional enrichment analysis showed that these lncrnas may affect the development of cancer through regulating oxidative stress response, cell cycle and apoptosis. The interaction network of ribosomal protein L34 reveals its central role in protein synthesis and cellular stress response.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140145"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive peptides with antioxidant and ACE inhibitory properties in goat milk protein hydrolysates: Peptidomics and molecular docking study.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-23 DOI: 10.1016/j.ijbiomac.2025.140286

Wenhua Zhang, Mohamed Aamer Abubaker, Zekun Li, Yu He, Qin Shu, Linqiang Li, Yongfeng Liu

引用次数: 0