International Journal of Biological Macromolecules最新文献

{"title":"α-Glucan rich polysaccharide fraction obtained from rhizomes of Kaempferia parviflora (black ginger) exhibits immunostimulatory activity in RAW264.7 cells","authors":"ChangSheng Li ,&nbsp;Fang Kou ,&nbsp;Rajavel Arumugam Uthamapriya ,&nbsp;Periyannan Rajasekar ,&nbsp;Jeong Seon Hwang ,&nbsp;Narayanasamy Marimuthu Prabhu ,&nbsp;Dong-Jin Lee ,&nbsp;Subramanian Palanisamy ,&nbsp;SangGuan You ,&nbsp;Jin-Chul Kim","doi":"10.1016/j.ijbiomac.2025.146027","DOIUrl":"10.1016/j.ijbiomac.2025.146027","url":null,"abstract":"<div><div>This study investigated the structural characteristics and immunostimulatory activities of polysaccharides, primarily composed of α-glucan, isolated from the rhizomes of <em>Kaempferia parviflora</em> (black ginger), with a focus on F2 using RAW264.7 cells. The extracted and purified polysaccharides yielded two fractions, F1 and F2. The primary components of these fractions included carbohydrates (84.47 ± 4.33 and 45.69 ± 2.26%), protein (2.72 ± 0.21% and 29.14 ± 0.60%), and sulfates (8.89 ± 0.23 and 23.47 ± 1.72%), with glucose being the dominant monosaccharide (98.7 ± 0.38 and 77.5 ± 0.28%), followed by arabinose (0.5 ± 0.02 and 9.8 ± 0.44%) and galactose (0.2 ± 0.01 and 7.6 ± 0.25%), along with minor amounts of rhamnose, xylose, and mannose. F2 is rich in α-glucans with a predominant →4)-Glc-(1 → linkage, typical of unbranched glucan chains. It has a highly branched structure with (1 →)-, (1 → 4), (1 → 2), (1 → 6), (1 → 2,3), and (1 → 3)-linked glucopyranose residues, arabinose, galactose, and rhamnose linkages. The F2 fraction significantly increased NO production and upregulated macrophage surface receptor expression, thereby activating the NF-κB and MAPK signaling pathways. The study confirms that the immunostimulatory activity observed in <em>K. parviflora</em> rhizomes is primarily due to the polysaccharide component, suggesting its potential as a natural immunotherapeutic agent.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"319 ","pages":"Article 146027"},"PeriodicalIF":8.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Transformative lactera-polypyrrole@carrageenan microparticles leveraging NIR for skin regeneration and stress relief\" [Int. J. Biol. Macromol. 2025 May 20: 144436].","authors":"Sih-Chi Chuang, Shih-An Yu, Pei-Chia Hung, Andrew E-Y Chuang, Jia-Wei Liang, Lekshmi Rethi, Chih-Kuang Chen, Hsu-Wei Fang, Hieu Trung Nguyen, Hsien-Tsung Lu","doi":"10.1016/j.ijbiomac.2025.145949","DOIUrl":"10.1016/j.ijbiomac.2025.145949","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145949"},"PeriodicalIF":8.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Activity and stability improvement: structure-function insights into CotA from Bacillus subtilis” [Int. J. Biol. Macromol. 318 (2025) 145156]","authors":"Wei He,&nbsp;Yiru Wang,&nbsp;Xizhen Ge,&nbsp;Ying Li","doi":"10.1016/j.ijbiomac.2025.145952","DOIUrl":"10.1016/j.ijbiomac.2025.145952","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"319 ","pages":"Article 145952"},"PeriodicalIF":8.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glyphosate targets FYN to regulate glycolysis and promote glioblastoma proliferation: A network toxicology study","authors":"Anquan Ma ,&nbsp;Ziqing Yang ,&nbsp;Qixuan He ,&nbsp;Wenhao Wang ,&nbsp;Huiping Ren ,&nbsp;Chuanyao Zhai ,&nbsp;Jing Lan","doi":"10.1016/j.ijbiomac.2025.146486","DOIUrl":"10.1016/j.ijbiomac.2025.146486","url":null,"abstract":"<div><div>Environmental glyphosate exposure has been linked to glioblastoma (GBM), yet its molecular basis remains unclear. Integrating network-toxicology and druggable Mendelian randomization screens, we identified the Src-family kinase FYN as the principal glyphosate target. Molecular-dynamics simulations, surface-plasmon resonance (KD = 1.54 μM) and pull-down assays confirmed high-affinity binding and highlighted ASP353 as a dominant contact residue. Multi-omics profiling showed FYN over-expression and promoter hypomethylation in GBM, correlating with diminished immune infiltration. In U87 cells, sub-toxic glyphosate (0.1 mg/L, 12 h) up-regulated FYN, activated PI3K–AKT–mTOR signaling, increased GLUT1, LDHA and PKM2, and accelerated proliferation, migration and invasion; lentiviral sh-FYN reversed these effects and curtailed glycolytic flux. Orthotopic mouse studies mirrored the in-vitro findings, with FYN knock-down suppressing glyphosate-driven tumor growth. Exosomes derived from sh-FYN glioma cells weakened macrophage M2 polarization and reduced CXCL1, IL-10 and TGF-β secretion, revealing an immunometabolism axis. Collectively, these results establish FYN as the mechanistic conduit between glyphosate and GBM and demonstrate that targeting FYN—directly or via exosome delivery—reprograms tumor glycolysis and immunity, offering a tractable strategy against glyphosate-associated malignancy.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146486"},"PeriodicalIF":8.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"nZVI induced cellulose nanocrystals/poly(acrylic acid) cross-linked hydrogel for enhanced diclofenac adsorption","authors":"Luna Tie ,&nbsp;Xiao Zhou ,&nbsp;Yucai Bai ,&nbsp;Mingyang Song ,&nbsp;Xiaodong Li ,&nbsp;Wei-xian Zhang ,&nbsp;Zilong Deng","doi":"10.1016/j.ijbiomac.2025.146495","DOIUrl":"10.1016/j.ijbiomac.2025.146495","url":null,"abstract":"<div><div>Conventional adsorbents face significant challenges when treating wastewater contaminated with organic pollutants due to their high operating costs, limited removal efficiency, and risk of secondary pollution. In this study, a sustainable nanoscale zero-valent iron (nZVI) induced cellulose nanocrystals/poly(acrylic acid) cross-linked hydrogel (Fe@CP) was prepared by free radical polymerization and ion-crosslinking. The nZVI crosslinker is gradually oxidized to Fe³⁺ by the free radicals generated by the initiator. The released Fe³⁺ coordinated with the negatively charged carboxyl and sulfate half ester groups on the CP chains, thereby forming a uniform crosslinked network. The adsorption kinetics of diclofenac (DCF) onto Fe@CP followed the pseudo-second-order model (R² &gt; 0.963), suggesting chemical adsorption plays a dominant role in the process. Furthermore, the adsorption isotherm for DCF on Fe@CP were well-described by Langmuir model, indicating that monolayer adsorption occurs on the adsorbent surface. The maximum adsorption capacity and rate constant of Fe@CP are 801.5 mg·g⁻¹ and 0.065 L·mg⁻¹, higher than CP hydrogel crosslinked directly with Fe³⁺ or Fe₃O₄. The Fe@CP hydrogel shows excellent DCF adsorption performance due to nZVI crosslinking, interconnected channels and abundant active sites. In addition, the wide pH adaptability, high ionic strength resistance and ionic coexistence provide great potential for pharmaceutical wastewater applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146495"},"PeriodicalIF":8.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Review on the synergistic mechanisms in harnessing rice residue-derived cellulose nanocrystals for sustainable water purification and wastewater treatment\" [Int. J. Biol. Macromol. Volume 311, Part 1, June 2025, 143225].","authors":"Bhupinder Singh, Ravinder Kumar, Shubham Sharma, T Ramachandran, V K Bupesh Raja, Abinash Mahapatro, Deepak Gupta, Ankit Kedia, A I Ismail, Abhinav Kumar","doi":"10.1016/j.ijbiomac.2025.145955","DOIUrl":"10.1016/j.ijbiomac.2025.145955","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145955"},"PeriodicalIF":8.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, purification, and biochemical characterization of glucose oxidase from the newly isolated Talaromyces pinophilus 47 K9, an endophytic fungus from Prosopis stephaniana","authors":"Aminallah Tahmasebi ,&nbsp;Byambasuren Ganbaatar ,&nbsp;Somayeh Bakhshi ,&nbsp;Hyosuk Yun ,&nbsp;Chul Won Lee","doi":"10.1016/j.ijbiomac.2025.146402","DOIUrl":"10.1016/j.ijbiomac.2025.146402","url":null,"abstract":"<div><div>Endophytic fungi have emerged as valuable reservoirs for discovering bioactive compounds with significant biological properties. In this study, a novel endophytic fungus, <em>Talaromyces pinophilus</em> 47 K9, was isolated from <em>Prosopis stephaniana</em>. The antimicrobial enzyme glucose oxidase was purified from the culture medium of <em>T. pinophilus</em> 47 K9 through a series of techniques, including 70 % ammonium sulfate precipitation, solid-phase extraction, ultrafiltration, ion-exchange chromatography, and gel filtration. The enzyme was identified using SDS-PAGE and proteomic analysis via MS/MS sequencing. Biochemical characterization revealed a molecular weight of 65.8 kDa and an enzymatic activity of 0.71 U/mL when glucose served as a substrate. The glucose oxidase demonstrated broad stability within a pH range of 3 to 10, though it was thermally unstable at temperatures exceeding 60 °C. Substrate specificity tests indicated that glucose served as the optimal substrate for H₂O₂ production, which subsequently improved antimicrobial efficacy. Notably, the glucose oxidase exhibited broad-spectrum antimicrobial activity, effectively inhibiting five <em>Candida</em> species, six phytopathogenic fungi, and both Gram-positive and Gram-negative bacteria. Given its potent antimicrobial properties, high substrate specificity, and stability across diverse conditions, glucose oxidase from <em>T. pinophilus</em> 47 K9 holds potential as a viable alternative antimicrobial agent.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146402"},"PeriodicalIF":8.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel glucomannan from Bletilla striata ameliorates colitis: Restores intestinal barrier, alleviates inflammation, and modulates the gut flora","authors":"Xialin Sun ,&nbsp;Tingwen Zhang ,&nbsp;Yan Zhao ,&nbsp;Han Yang ,&nbsp;Yawei Li ,&nbsp;Xin Sun ,&nbsp;Xingbo Bian","doi":"10.1016/j.ijbiomac.2025.146421","DOIUrl":"10.1016/j.ijbiomac.2025.146421","url":null,"abstract":"<div><h3>Background</h3><div>Ulcerative colitis (UC) involves intestinal barrier dysfunction, immune dysregulation, and microbiota imbalance. <em>Bletilla striata</em> polysaccharide (BSP) shows anti-inflammatory potential, but its UC mechanisms remain unclear.</div></div><div><h3>Purpose</h3><div>To purify <em>Bletilla striata</em> polysaccharide (BSP-1), characterize its structure, and elucidate its multi-target UC therapeutic mechanisms.</div></div><div><h3>Methods</h3><div>This study purified a novel structure of neutral glucomannan with a small amount of acetylation (BSP-1). Together with tight junction protein analysis, oxidative stress markers, 16S rRNA sequencing, cytokine quantification, and molecular ecological network examination, the team used a UC mouse model created using dextran sodium sulfate (DSS).</div></div><div><h3>Results</h3><div>BSP-1 alleviated colitis by inhibiting TLR4/MyD88/NF-κB signaling (elevating IL-10; lowering IL-6, TNF-α, and IL-1β) and restoring integrity of the intestinal barrier via upregulating Occludin, ZO-1, MUC2, and Claudin-1. It reduced oxidative stress (lower MDA; higher SOD) and modulated gut microbiota (enriched <em>Lactobacillus</em>, suppressed <em>Escherichia</em>-<em>Shigella</em>). Molecular ecological network analysis showed that BSP-1 restored microbial community stability by enhancing bacterial competition.</div></div><div><h3>Conclusion</h3><div>BSP-1 exerts integrated anti-inflammatory, barrier-repairing, and microbiota-regulating effects, highlighting its potential as a multi-target UC therapy and intestinal microecological regulator.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146421"},"PeriodicalIF":8.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on keratin-based sensor platforms: Structural properties of keratin, their role in analytical sensing applications, and future perspectives","authors":"Selva Bilge ,&nbsp;Cem Erkmen ,&nbsp;Abdullah Yücel","doi":"10.1016/j.ijbiomac.2025.146469","DOIUrl":"10.1016/j.ijbiomac.2025.146469","url":null,"abstract":"<div><div>Keratin, a fibrous structural protein abundant in biological materials, has recently garnered attention as a novel and environmentally friendly material for sensor platforms due to its diverse properties and biocompatibility. This review provides a comprehensive and up-to-date overview of keratin-based sensor platforms, emphasizing the structural features, such as its complex disulfide (-S-S) bonding network and ability to form nanofibrous matrices, that make keratin particularly suitable for analytical sensing applications. The sensing performance of keratin-based materials is critically evaluated, with a focus on their sensitivity and selectivity toward various analytes, including pharmaceutical compounds, proteins, and heavy metals. Particular attention is given to the mechanisms by which keratin interacts with analytes to produce measurable electrical, optical, or mechanical signals. Furthermore, the review discusses current limitations, including the need for improved mechanical stability and long-term durability, and outlines strategies for functional modification. The review also addresses fabrication techniques such as electrospinning and layer-by-layer assembly used to integrate keratin into sensor platforms. Additionally, the potential for incorporating keratin-based sensors into wearable and implantable devices is explored, with a focus on their applications in medical diagnostics, environmental monitoring, and food safety. Unlike previous reviews that primarily address keratin's general biomaterial applications, this review presents a focused and in-depth analysis of keratin's role specifically in sensor technology, showcasing the most recent advances, fabrication strategies, and practical implementations. The review uniquely bridges the gap between fundamental keratin chemistry and real-world sensor development, offering novel insights into how keratin's molecular versatility can be harnessed to design next-generation, eco-friendly, and biocompatible sensing platforms.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146469"},"PeriodicalIF":8.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orange debittering by a novel thermostable flavonoid glycosidase","authors":"Yaoguang Huang ,&nbsp;Yingjun Liu ,&nbsp;Haijiao Wang ,&nbsp;Bao Yang","doi":"10.1016/j.ijbiomac.2025.146403","DOIUrl":"10.1016/j.ijbiomac.2025.146403","url":null,"abstract":"<div><div>Naringin, a bitter flavanone glycoside in <em>citrus</em> with diverse bioactivities, consists of a glycosyl group linked to naringenin. Naringinase-catalyzed hydrolysis cleaves this glycosidic bond, converting bitter naringin into tasteless naringenin. Current naringinases suffer from limited diversity, poor thermostability, and narrow substrate specificity. While the thermostable glycosidase from <em>Pyrococcus furiosus</em> enables broad-spectrum debittering via transglycosylation, its low catalytic efficiency and complex purification hinder practical application. This study employed semi-rational design to engineer PfNG. Structural analysis yielded single mutants (F265A, M328S) and combinatorial mutant V1, exhibiting optimal activity at 95 °C with a 2.48-fold higher <em>K</em>cat/<em>K</em>m than wild-type. We further developed a streamlined one-step thermal purification protocol, providing an efficient alternative to chromatography. Structural characterization revealed enhanced activity likely originates from: (1) contracted binding pocket volume, (2) widened substrate channel entrance, (3) novel hydrogen bonds, and (4) increased conformational flexibility. V1 effectively reduced naringin content (&gt;91 %) in green <em>citrus</em> extracts and mitigated juice bitterness. Our work resolves key limitations of PfNG, advancing its industrial deployment for <em>citrus</em> debittering and biopharmaceutical applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146403"},"PeriodicalIF":8.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}