International Journal of Biological Macromolecules最新文献

{"title":"Corrigendum to \"Characterization and functional properties of walnut protein fibrils for enhanced bioaccessibility of CoQ10 and ALA\" [International Journal of Biological Macromolecules, Volume 285 (2025), 138171].","authors":"Xiaoyu He, Li Zhang, Minjie Cao, Yiwen Guo, Jie Mi, Zhu Zhu, Yuehao Song, Ming Chang, Ruijie Liu, Xingguo Wang","doi":"10.1016/j.ijbiomac.2025.139960","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139960","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"139960"},"PeriodicalIF":7.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation into Alzheimer's-related amyloid-β conformational transformations and stability influenced by green iron oxide nanoparticles (GIONP).","authors":"Heba A Esawii, Kahramon Mamatkulov, Hanan A Mahran, Grigory Arzumanyan, Noha Mohamed","doi":"10.1016/j.ijbiomac.2025.140124","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140124","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is popularly believed to be triggered by the aggregation of amyloid beta 1-42 (Aβ - 42) peptides, eventually leading to neurodegeneration. Our study delves into the influential role played by Green Iron Oxide Nanoparticles (GIONP). GIONP are typically synthesized using a green chemistry approach, imposing curcumin as a biocompatible reducing and capping agent, leveraging its inherent antioxidant, anti-inflammatory, and neuroprotective attributes. Herein, our research particularly aims to decipher whether GIONP modulates the secondary structure of Aβ1-42 peptides with a close consideration to the surrounding physiological factors, as well as the membrane bilayer probable conformation changes. Raman spectroscopy was employed to investigate the interaction between GIONP and Aβ1-42 aggregates, demonstrating significant alterations in secondary structure dynamics of Aβ1-42 polypeptide. Fourier-transform infrared (FTIR) spectroscopy shed light on the chemical interactions between GIONP and curcumin, a capping agent. X-ray diffraction (XRD) analysis was performed to determine the crystalline structure and phase purity of the synthesized GIONP, providing insights into their stability and structural integrity. GIONP particle size distribution investigations and membrane architectures surrounding GIONP were carried out for their impact on membrane integrity and stability. The morphology of GIONP, membrane mimetic liposomal structures formation, and integrity were studied using transmission electron microscopy (TEM), accompanied with energy-dispersive X-ray spectroscopy (EDS), which displayed the elements distribution within each of the structures. The study uncovered that GIONP stabilizes the secondary structure of Aβ1-42, potentially offering modulation to the aggregation process. Furthermore, GIONP proved to have no negative impact on membrane integrity, implying that they could be safely employed as a therapeutic option for the modulation of peptide aggregation's pathological pathway of Alzheimer's disease. This study may contribute to broadening our understanding of nanoparticle-mediated therapies in modulating neurodegenerative disorders, highlighting their dual involvement in amyloid aggregation regulation and membrane structure maintenance.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"140124"},"PeriodicalIF":7.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Bovine serum albumin-liposome stabilized high oil-phase emulsion: Effect of liposome ratio on interface properties and stability\" [Int. J. Biol. Macromol. 266 (2024) 131040].","authors":"Yang Chen, Xiangzhou Yi, Zhisheng Pei, Xuan Zhang, Xia Gao, Weimin Zhang, Xuanri Shen","doi":"10.1016/j.ijbiomac.2025.139781","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139781","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"139781"},"PeriodicalIF":7.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chitosan-gentianic acid derivatives on myofibrillar proteins in sea bass (Lateolabrax maculatus) during refrigerated storage.","authors":"Weiqing Lan, Mingxing Zhou, Bingjie Zhang, Shucheng Liu, Jing Xie","doi":"10.1016/j.ijbiomac.2025.140107","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140107","url":null,"abstract":"<p><p>Phenolic acid-chitosan derivatives have received extensive attention due to their greatly enhanced mechanical, antibacterial and antioxidant properties, especially in food preservation. The chitosan-gentianic acid (CS-g-GA) was prepared and its impact on myofibrillar proteins (MPs) in sea bass (Lateolabrax maculatus) during refrigerated storage was investigated in this study. Fish fillets were immersed in distilled water, CS, GA and CS-g-GA solutions, respectively, followed by an 18-day refrigerated storage. MPs were extracted from fish fillets every 3 days, and then the degree of oxidation and conformational changes of MPs were evaluated. The results of myofibril fragmentation index (MFI) and surface hydrophobicity confirmed that CS-g-GA can retard the fragmentation and degradation of myofibrils. Additionally, the total sulfhydryl content of CS-g-GA-treated samples in the later storage was significantly higher than those of other groups, confirming that CS-g-GA treatment protected the structure and function of protein. Results of Fourier transform infrared spectroscopy (FT-IR) and inner fluorescence intensity (IFI) showed that CS-g-GA maintained the secondary and tertiary structure of MPs. The results from scanning electron microscope (SEM) further clarified that CS-g-GA can maintain the tight arrangement of muscle fibers and protect the microstructure of myofibrils. Furthermore, the observation of confocal laser scanning microscopy (CLSM) demonstrated that CS-g-GA can prevent protein cross-linking and aggregation. Therefore, CS-g-GA treatment can effectively protect the structure and delay the oxidation of MPs.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140107"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing emulsification properties of pea protein isolate: Impact of heat treatment and soy hull polysaccharides on conformational modification and stability.","authors":"Xiuzhi Cao, Shengnan Wang, Yunfei Yu, Lu Han, He Liu","doi":"10.1016/j.ijbiomac.2025.140106","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140106","url":null,"abstract":"<p><p>In order to investigate the effect of conformational change in pea protein isolate (PPI) on its emulsification properties, soy hull polysaccharides (SHP) were added to modify the conformation following heat treatment at 70-100 °C to improve emulsification. The results of UV and fluorescence spectroscopy indicated that the heat treatment exposed the amino acid residues to a more hydrophobic environment. The mean volume diameter (d_4,3) of PPI was reduced from 67.25 ± 3.31 to 45.50 ± 0.62 μm, and secondary structure of protein became more ordered. The addition of SHP enhanced the adsorption of protein at the oil-water interface and reduced the interfacial tension. Interestingly, SHP decreased the short-term (12h) thermal stability index (TSI) from 3.5 to 2.8 in PPI/SHP emulsion treated at 100 °C. These findings validated that heat treatment combined with SHP modification can improve the emulsification of PPI, which positively impacts the development of pea-based products for high temperatures applications.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140106"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ternary deep eutectic solvent for efficient biomass fractionation and lignin stabilization.","authors":"Yao Zheng, Pengcheng Xue, Rong Guo, Jianyu Gong, Guangfu Qian, Changzhou Chen, Douyong Min, Yan Tong, Minsheng Lu","doi":"10.1016/j.ijbiomac.2025.140070","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140070","url":null,"abstract":"<p><p>The efficient isolation and lignin stabilization are critical to the fractionation process of lignocellulosic biomass, enabling the subsequent valorization of both carbohydrates and lignin. In this study, a ternary deep eutectic solvent pretreatment system with outstanding reusability has been developed. Under optimal conditions (ChCl: MT: p-TsOH = 1:1:0.5, 120 °C, 60 min), the system efficiently removed 94.66 % of hemicellulose and 95.74 % of lignin while retaining 84.50 % of cellulose. Glucose was obtained from the cellulose-rich solid residue via enzymatic hydrolysis, achieving an 87.12 % yield. This DES system inhibits lignin condensation through a dual mechanism of α-etherification and intermolecular forces (π-π stacking and hydrophobic interaction). The recovered lignin exhibits a low molecular weight (922-1049 g/mol), high phenolic hydroxyl content (2.57-3.37 mmol/g), low polydispersity (1.54-1.61), and high purity (93.02 %). Combined with its superior antioxidant activity and UV shielding properties, this lignin represents a promising new resource with potential applications.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140070"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolved enzymes in the metabolism of biological poly-acids: Applications in otolaryngological biocatalysis.","authors":"Shanshan Li, Wei Wang, Shengnan Liu, Yaqi Du, Ning Zhao","doi":"10.1016/j.ijbiomac.2025.140068","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140068","url":null,"abstract":"<p><p>This study explores evolved Hyaluronidase, Lipase, and Elastase's identification, characterization, and therapeutic potential to enhance tissue regeneration and drug delivery systems in otolaryngology. Hyaluronidase variant H5 exhibited a turnover number (k_cat) of 1500 min^-1, a 200 % increase over wild-type (500 min^-1), demonstrating superior hyaluronic acid degradation. Similarly, lipase variant L2 reached 1200 min^-1 (400 min^-1 wild-type), and elastase variant E3 showed a turnover of 2200 min^-1 (1000 min^-1 wild-type). Kinetic analyses revealed improved Km and Vmax values across variants, with Hyaluronidase Variant H5 achieving Km = 1.5 μM and Vmax = 3000 μM/min. Molecular Dynamics (MD) simulations indicated structural stability (average RMSD ~1.5 Å for H5) and strong hydrogen bonding (180 bonds), enhancing catalytic efficiency. In vitro assays demonstrated a 40 % enhancement in tissue regeneration and increased epithelial cell proliferation (100 % for Hyaluronidase Variant H5 vs. 60 % wild-type). In vivo studies in rabbits revealed a 30 % reduction in recovery time post-sinus surgery and a 50 % reduction in scar tissue formation. These findings underscore the potential of evolved enzymes in advancing drug delivery (DD) and tissue repair (TR), with implications for broader applications in wound healing and inflammatory diseases.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140068"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CEBPA as a potential hub gene for cutaneous inflammation in type 2 diabetes mellitus.","authors":"Mingzheng Han, Jingchun Wang, Yijin Wu, Jianzhao Liao, Jianying Guo, Zhaoxin Tang","doi":"10.1016/j.ijbiomac.2025.140080","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140080","url":null,"abstract":"<p><strong>Background: </strong>The role of inflammation in the development of type 2 diabetes mellitus (T2DM) related skin complications necessitates further investigation. This study aims to explore the correlation between inflammation and cutaneous alterations in T2DM, enhancing comprehension of underlying mechanism involved.</p><p><strong>Methods: </strong>Utilizing bioinformatics, the GSE38396 and GSE92724 datasets were employed to identify differentially expressed genes (DEGs) and potential hub genes in T2DM-related skin inflammation. Subsequently, gene functional enrichment analysis was employed for functional annotation. Finally, we validated the regulatory impact of hub gene on inflammation during high glucose incubation using the in vitro model.</p><p><strong>Results: </strong>A comprehensive analysis identified 742 DEGs, including 9 hub genes and 4 potential biomarkers. Compared to the CON group, the expression of M2 macrophages was significantly upregulated in the T2DM group, while resting dendritic cells and eosinophils showed notable decreases, indicating a significant correlation with CEBPA. Furthermore, functional enrichment analysis revealed significant enrichment of DEGs in pathways linked to immunity and diabetes pathogenesis. Interestingly, overexpression of CEBPA demonstrated anti-inflammatory effects under hyperglycemic conditions, while silencing CEBPA expression appeared to worsen inflammation.</p><p><strong>Conclusion: </strong>CEBPA emerges as a potential hub gene for skin inflammation in T2DM, shedding light on the underlying mechanisms of this condition.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140080"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioelectronics hydrogels for implantable cardiac and brain disease medical treatment application.","authors":"Yanfang Meng, Lin Xu, Guanggui Cheng","doi":"10.1016/j.ijbiomac.2025.139945","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139945","url":null,"abstract":"<p><p>Hydrogel-based bioelectronic systems offer significant benefits for point-of-care diagnosis, treatment of cardiac and cerebral disease, surgical procedures, and other medical applications, ushering in a new era of advancements in medical technology. Progress in hydrogel-based bioelectronics has advanced from basic instrument and sensing capabilities to sophisticated multimodal perceptions and feedback systems. Addressing challenges related to immune responses and inflammation regulation after implantation, physiological dynamic mechanism, biological toxicology as well as device size, power consumption, stability, and signal conversion is crucial for the practical implementation of hydrogel-based bioelectronics in medical implants. Therefore, further exploration of hydrogel-based bioelectronics is imperative, and a comprehensive review is necessary to steer the development of these technologies for use in implantable therapies for cardiac and brain/neural conditions. In this review, a concise overview is provided on the fundamental principles underlying ionic electronic and ionic bioelectronic mechanisms. Additionally, a comprehensive examination is conducted on various bioelectronic materials integrated within hydrogels for applications in implantable medical treatments. The analysis encompasses a detailed discussion on the representative structures and physical attributes of hydrogels. This includes an exploration of their intrinsic properties such as mechanical strength, dynamic capabilities, shape-memory features, stability, stretchability, and water retention characteristics. Moreover, the discussion extends to properties related to interactions with tissues or the environment, such as adhesiveness, responsiveness, and degradability. The intricate relationships between the structure and properties of hydrogels are thoroughly examined, along with an elucidation of how these properties influence their applications in implantable medical treatments. The review also delves into the processing techniques and characterization methods employed for hydrogels. Furthermore, recent breakthroughs in the applications of hydrogels are logically explored, covering aspects such as materials, structure, properties, functions, fabrication procedures, and hybridization with other materials. Finally, the review concludes by outlining the future prospects and challenges associated with hydrogels-based bioelectronics systems.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139945"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Konjac glucomannan/zein active film loaded with tea polyphenol-ferric nanoparticles for strawberry preservation.","authors":"Zhenzhen Wu, Lin Wang, Zhiwen Hu, Xiuqing Guan, Yibin Chen, Mingming Xu, Xianrui Chen, Nitong Bu, Jie Duan, Wei Liu, Chen Ma, Jie Pang","doi":"10.1016/j.ijbiomac.2025.139905","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139905","url":null,"abstract":"<p><p>With increasing global environmental awareness and concerns about food safety, biodegradable active packaging has garnered widespread attention. In this study, the stability and bioactivity of tea polyphenol (TP) were enhanced through the preparation of TP-ferric nanoparticles (TP-Fe NPs) using metal-polyphenol ion coordination. Moreover, the introduction of Fe ions can further enhance the antibacterial effects of NPs. Using the hydrogen bonding between konjac glucomannan (KGM) and zein to enhance the hydrophobicity and mechanical properties of the film. By employing KGM and zein as the matrix, we incorporated TP-Fe NPs as active fillers to create multifunctional active packaging films. This study aimed to meet the needs of food safety and sustainable development goals. The resulting film exhibited excellent water resistance (water contact angle: 117.73°), mechanical strength (tensile strength: 21.82 MPa, elongation at break: 94.30 %), ultraviolet-shielding ability (>99 %), biodegradability (5 days in soil), and antioxidant (>85 %) and antibacterial (>99 %) properties. Moreover, the film significantly reduced strawberry decay and extended its shelf life by 10 days. These findings provide new insights into the application of nanomaterials in active packaging, highlighting their potential and advantages in food preservation.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139905"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}