International Journal of Biological Macromolecules最新文献

Corrigendum to “Impact of salting-in/out assisted extraction on rheological, biological, and digestive, and proteomic properties of Tenebrio molitor larvae protein isolates” [Int. J. Biol. Macromol. 282 (2024) 137044] 盐入/盐出辅助萃取对天牛幼虫蛋白质分离物的流变学、生物学、消化和蛋白质组学特性的影响》更正[Int. J. Biol. Macromol.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-18 DOI: 10.1016/j.ijbiomac.2024.137673

Yongli Jiang , Qi Tian , Chongyang Chen , Yun Deng , Xiaosong Hu , Junjie Yi

引用次数: 0

Significant enhancement of the thermal stability and catalytic efficiency of transglutaminase in Streptomyces mobaraensis engineered through the novel S. mobaraensis genomic mutant library construction method GHR/Sml 通过新型 S. mobaraensis 基因组突变体文库构建方法 GHR/Sml，显著提高了莫巴拉链霉菌转谷氨酰胺酶的热稳定性和催化效率。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137733

Fang Yuan , Guoying Li , Zilong Li , Mingming Li , Haiquan Yang , Xiaobin Yu

{"title":"Significant enhancement of the thermal stability and catalytic efficiency of transglutaminase in Streptomyces mobaraensis engineered through the novel S. mobaraensis genomic mutant library construction method GHR/Sml","authors":"Fang Yuan , Guoying Li , Zilong Li , Mingming Li , Haiquan Yang , Xiaobin Yu","doi":"10.1016/j.ijbiomac.2024.137733","DOIUrl":"10.1016/j.ijbiomac.2024.137733","url":null,"abstract":"<div><div>Herein, we developed a novel Streptomyces mobaraensis genomic mutant library construction method, GHR/Sml, to directly and significantly enhance the thermal stability and catalytic efficiency of TGase in the genome of S. mobaraensis. First, 13 key amino acid residues and their mutations for enhanced thermal stability were identified using error-prone PCR and site-directed mutagenesis. Then, the GHR/Sml method was developed to construct a TGase genomic mutant library with 13 mutations. Positive mutants S23Y/Y24N/S250R, S23Y/Y24N/S303K, S23Y/Y24N/K294L, S23Y/Y24N/S199A/R208L, S23Y/Y24N, and S250R were obtained from 1500 total mutants; their half-life values at 50 °C were increased by 9.3-, 9.5-, 8.7-, 9.0-, 6.9-, and 4.8-fold compared with that of TGLD, respectively. Furthermore, the kcat/Km of mutant S23Y/Y24N/S250R increased by 1.25-fold over that of TGLD. The activity of S23Y/Y24N/S250R reached 65.34 U/mL in a 1000-L fermenter, which was the highest activity reported. This novel GHR/Sml method is of great significance for systematically improving properties of additional enzymes in the genome of S. mobaraensis.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"283 ","pages":"Article 137733"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carboxymethylcellulose-based aggregation-induced emission antibacterial material for multifunctional applications 基于羧甲基纤维素的聚集诱导发射抗菌材料的多功能应用。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137740

Jingfei Wang , Meiyi Wu , Ruiting Zhang , Chongruihan Li , Chaoqun Li , Shuangling Zhong , Yan Gao , Qingye Meng , Xuejun Cui

{"title":"Carboxymethylcellulose-based aggregation-induced emission antibacterial material for multifunctional applications","authors":"Jingfei Wang , Meiyi Wu , Ruiting Zhang , Chongruihan Li , Chaoqun Li , Shuangling Zhong , Yan Gao , Qingye Meng , Xuejun Cui","doi":"10.1016/j.ijbiomac.2024.137740","DOIUrl":"10.1016/j.ijbiomac.2024.137740","url":null,"abstract":"<div><div>Polysaccharides are ubiquitous in nature, typically harmless, and highly compatible with various tissues in biomedical contexts. These properties make them attractive for use in multifunctional materials. In this study, the aggregation-induced emission (AIE) antibacterial material (PLOCMC) was successfully synthesized by carboxymethylcellulose (CMC) and ε-Poly-Lysine (ε-PL). PLOCMC exhibits not only the AIE property but also a room temperature phosphorescent (RTP) phenomenon. This dual emission behavior enhances its potential applications in chemical sensing and anti-counterfeiting. Notably, PLOCMC shows low cytotoxicity and exhibits antibacterial activity against typical Gram-positive and Gram-negative bacteria, making it a potent agent against a variety of bacterial strains. Additionally, PLOCMC demonstrates specific responsiveness to Fe3+ ions and nitrite, indicating its potential utility in food safety and monitoring applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"283 ","pages":"Article 137740"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of HPMC/NaAlg-CuO bio-nanocomposites: Enhanced optical, electrical, and antibacterial properties for sustainable packaging applications. HPMC/NaAlg-CuO 生物纳米复合材料的开发与表征：增强可持续包装应用的光学、电学和抗菌性能。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137774

H M Ragab, N S Diab, Rosilah Ab Aziz, Eshraga Abdallah Ali Elneim, Azzah M Alghamdi, Sara A Al-Balawi, M O Farea

{"title":"Development and characterization of HPMC/NaAlg-CuO bio-nanocomposites: Enhanced optical, electrical, and antibacterial properties for sustainable packaging applications.","authors":"H M Ragab, N S Diab, Rosilah Ab Aziz, Eshraga Abdallah Ali Elneim, Azzah M Alghamdi, Sara A Al-Balawi, M O Farea","doi":"10.1016/j.ijbiomac.2024.137774","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137774","url":null,"abstract":"Copper oxide nanoparticles (CuO NP) were incorporated into a hydroxypropyl cellulose (HPMC) and sodium alginate (NaAlg) matrix through a casting method to create bio-nanocomposite films. XRD analysis confirmed the semi-crystalline nature of the HPMC/NaAlg matrix, with a broad peak at 2θ = 21.22°, which decreased in intensity as CuO concentration increased, indicating a shift towards an amorphous structure. FT-IR analysis demonstrated changes in band intensity, which can be attributed to the reduced volume fraction of the polymer blend in the presence of the CuO nanofiller. SEM images showed homogeneity at low CuO NP concentrations, but at 0.9 wt% CuO, nanoparticle aggregation became evident. The UV-visible spectra indicated a redshift from 212 nm to 246 nm and a decrease in optical energy gap from 4.78 eV for the pure blend to 2.99 eV at 0.9 wt% CuO, associated with increased localized defect states. AC electrical conductivity and dielectric properties improved with CuO dispersion, enhancing the bio-nanocomposite's suitability for electrochemical and optoelectronic applications. The bio-nanocomposites demonstrated significant antibacterial activity, with films containing 0.4 and 0.7 wt% CuO achieving the largest inhibition zones against B. subtilis, S. aureus, P. aeruginosa, and E. coli. Overall, these findings suggest that HPMC/NaAlg-CuO bio-nanocomposites are promising candidates for use in antibacterial packaging and optoelectronics.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137774"},"PeriodicalIF":7.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Saccharomyces cerevisiae accumulating excessive amount of glycogen and its effects on gut microbiota in a mouse model. 小鼠模型中积累过量糖原的酿酒酵母的发育及其对肠道微生物群的影响

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137589

So Young Ban, Da-Young Yun, Su-Jin Yeom, Hee-Gon Jeong, Jong-Tae Park

{"title":"Development of Saccharomyces cerevisiae accumulating excessive amount of glycogen and its effects on gut microbiota in a mouse model.","authors":"So Young Ban, Da-Young Yun, Su-Jin Yeom, Hee-Gon Jeong, Jong-Tae Park","doi":"10.1016/j.ijbiomac.2024.137589","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137589","url":null,"abstract":"Saccharomyces cerevisiae accumulates glycogen, a hyperbranched glucose polymer with multiple bio-functionalities. In this study, mutants of S. cerevisiae that accumulate excessive amounts of glycogen were developed through UV mutagenesis. From over 30,000 mutants, the mutant strain CEY1, which exhibited the highest glycogen production, was selected using iodine vapor screening. The glycogen structures of wild type (WT) and CEY1 were analyzed and found to be relatively similar in molecular weight, hydrodynamic diameter, and side-chain distribution. The glycogen from CEY1 contained long branches (DP >12) 23.6 % greater than those in Escherichia coli TBP38. In addition, WT and CEY1 glycogen showed 32 %-34 % digestibility, which is significantly lower than E. coli glycogen. The glycogen content in dried CEY1 cells was increased to 21.7 % during laboratory-scale fed-batch fermentation. Glycogen with a homogeneous structure was accumulated to 17.5 % (w/w dried cell), and the total glucan content was increased by 33.2 % during large-scale fed-batch fermentation. In a mouse model, a diet containing 30 % CEY1 increased the production of butyrate and populations of beneficial bacteria, including Bacteroides and Parabacteroides. Therefore, glycogen from CEY1 exhibits a distinct structure from other polysaccharides, with notably slow and low digestibility, thereby indicating its potential application as a dietary supplement.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137589"},"PeriodicalIF":7.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inspired by plant body frameworks bionics: Fabrication of self-healing polyvinyl alcohol/cellulose nanocrystals composite hydrogels reinforced by polyurethane sponges for flexible supercapacitors. 受植物体框架仿生学启发：自愈合聚乙烯醇/纤维素纳米晶体复合水凝胶的制作，聚氨酯海绵对其进行增强，用于柔性超级电容器。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137795

Qiming Yan, Yang Cao, Qihui Chen, Maochun Hong, Meng Zhou

{"title":"Inspired by plant body frameworks bionics: Fabrication of self-healing polyvinyl alcohol/cellulose nanocrystals composite hydrogels reinforced by polyurethane sponges for flexible supercapacitors.","authors":"Qiming Yan, Yang Cao, Qihui Chen, Maochun Hong, Meng Zhou","doi":"10.1016/j.ijbiomac.2024.137795","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137795","url":null,"abstract":"With the booming development of electronic technology, ultra-toughness and self-healing supercapacitors have drawn substantial attentions. In this work, inspired by plant body frameworks, a novel method was proposed to prepare self-healing conductive hydrogels based on self-healing polyurethane sponge (PUS) network. First, a self-healing PUS based on multiple hydrogen bonding interactions and disulfide bonds was prepared. Subsequently, PUS was combined with polyvinyl alcohol (PVA)/cellulose nanocrystals (CNF) composite hydrogels crosslinked by borate ester bonds and hydrogen bonding interactions to manufacture the sponge network reinforced self-healing conductive hydrogels. Due to the reinforcement of PUS, the composite hydrogels had excellent mechanical properties, with a tensile strength of 1.81 MPa and a compressive strength of 1.96 MPa. After 400 times of charge-discharge cycles under bending deformation, the supercapacitor could maintain 90.1 % of the original specific capacitance value. Furthermore, the hydrogels could be healed at room temperature due to the hydrogen bonds and reversible borate bonds in PVA/CNF matrix, as well as the disulfide bonds and multiple hydrogen bonds in PUS. The healed supercapacitor could maintain 75.2 % of the original specific capacitance value after 400 times of charge-discharge cycles. Therefore, the as-prepared self-healing and tough conductive hydrogels may have promising prospects in electronic devices.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137795"},"PeriodicalIF":7.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comments on "Extraction, purification, characteristics, bioactivities, prospects, and toxicity of Lilium spp. polysaccharides by Li et al. (2024) in Int. J. Biol. Macromol." 关于 "Li 等（2024 年）在 Int.J. Biol.Macromol.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137770

Armstrong Ighodalo Omoregie, Mansur Alhassan, Tariq Ouahbi

{"title":"Comments on \"Extraction, purification, characteristics, bioactivities, prospects, and toxicity of Lilium spp. polysaccharides by Li et al. (2024) in Int. J. Biol. Macromol.\"","authors":"Armstrong Ighodalo Omoregie, Mansur Alhassan, Tariq Ouahbi","doi":"10.1016/j.ijbiomac.2024.137770","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137770","url":null,"abstract":"Lilium spp. polysaccharides (LSPs) are gaining significant attention for their diverse health benefits, including antioxidant, antitumor, and antibacterial properties. This paper critically analyzes a recent comprehensive review by Li et al., published in International Journal of Biological Macromolecules, focusing on LSP extraction, purification, and health benefits. While the original review offers valuable insights, this critique identifies opportunities to strengthen the bibliometric analysis section. This study employs a comprehensive search strategy in Scopus using specific keywords and covering a broader time frame (1975-2023), revealing 94 research articles on LSPs. The critique proposes improvements to enhance transparency and impact, such as specifying search queries and Boolean operators used across databases, detailing selection criteria, and incorporating advanced analyses. This article discusses author keyword analysis, co-citation analysis of cited authors, and bibliographic coupling analysis of documents using VOSviewer software. The global landscape mapping of LSP relationships involving authors, countries, and keywords was determined using RStudio software. These refinements will provide a more robust foundation for understanding the LSP research landscape and future research directions, while also addressing common pitfalls and suggesting improvements in bibliometric analysis for future studies.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137770"},"PeriodicalIF":7.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Non-enzymatic cholesterol biosensor: Electrochemical sensing based on peptide-polylactic acid thin film” [Int. J. Biol. Macromol. 281 (2024) 136337] 非酶胆固醇生物传感器：基于多肽-聚乳酸薄膜的电化学传感" [Int. J. Biol. Macromol. 281 (2024) 136337]。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137553

Ana Lia Bernardo , Ahammed H.M. Mohammed-Sadhakathullah , Clotilde B. Angelucci , Francesc Estrany , Alessandro Berghella , Juan Torras , Elaine Armelin , Sergio Oddi , Enrico Dainese

引用次数: 0

Impact of Imperata Cylindrica polysaccharide on liver lipid metabolism disorders caused by hyperuricemia. 白茅多糖对高尿酸血症引起的肝脂代谢紊乱的影响

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137592

Wenchen Yu, Junwen Wang, Yi Xiong, Jiaren Liu, Denis Baranenko, Alejandro Cifuentes, Elena Ibañez, Yingchun Zhang, Weihong Lu

{"title":"Impact of Imperata Cylindrica polysaccharide on liver lipid metabolism disorders caused by hyperuricemia.","authors":"Wenchen Yu, Junwen Wang, Yi Xiong, Jiaren Liu, Denis Baranenko, Alejandro Cifuentes, Elena Ibañez, Yingchun Zhang, Weihong Lu","doi":"10.1016/j.ijbiomac.2024.137592","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137592","url":null,"abstract":"Elevated uric acid levels are associated with lipid metabolism disorders. The effects of Imperata cylindrica polysaccharide (ICPC-a) were explored using a hyperuricemia mouse model and a uric acid-induced HepG2 hepatocyte model. ICPC-a significantly improved total cholesterol, triglycerides, low-density lipoprotein levels, and hepatic lipid deposition in hyperuricemia mice. The liver/body weight ratio decreased, and markers of liver damage, inflammation, and dyslipidemia improved. Metabolomics analysis suggested that ICPC-a modulates lipid metabolism by influencing the glycerophospholipid pathway and the enzyme LPCAT3. Stable HepG2 cell lines with knocked-down LPCAT3 were constructed, and Western blot and RT-PCR were used to assess the impact of its knockdown on lipid metabolism under uric acid stimulation. In cells with reduced LPCAT3 expression, ICPC-a was still able to alleviate uric acid-induced lipid accumulation, though the effect was less pronounced compared to cells with normal LPCAT3 levels. However, the effectiveness was diminished compared to cells where LPCAT3 was not knocked down. These findings indicated that LPCAT3 was an important target through which ICPC-a regulated lipid metabolism disorders induced by hyperuricemia. These discoveries emphasized that ICPC-a, as a prebiotic, could modulate hepatic lipid accumulation and inflammation, contributing to the maintenance of hepatic lipid homeostasis.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137592"},"PeriodicalIF":7.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoelectrocatalytic degradation of hyaluronic acid and regulation effects of its degradation products on gut microbiota in vitro. 透明质酸的光催化降解及其降解产物对体外肠道微生物群的调节作用。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137813

Yunning Yang, Youxian Zhou, Linlin Wang, Chunqing Ai, Yinghuan Fu, Shuang Song

{"title":"Photoelectrocatalytic degradation of hyaluronic acid and regulation effects of its degradation products on gut microbiota in vitro.","authors":"Yunning Yang, Youxian Zhou, Linlin Wang, Chunqing Ai, Yinghuan Fu, Shuang Song","doi":"10.1016/j.ijbiomac.2024.137813","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137813","url":null,"abstract":"Hyaluronic acid (HA) has multiple biological activities which are closely related to its molecular weight. In the present study, the photoelectrocatalytic method was established for HA degradation and the influences of bias potentials, H2O2 additions and reaction times on the degradation results were investigated to optimize the reaction condition. Moreover, a series of analysis methods, such as FT-IR and NMR were used to analyze chemical compositions of the degradation products, revealing that photoelectrocatalytic degradation did not damage the structural blocks of HA obviously. Then 11 oligosaccharides with polymerization degrees from 2 to 8 in the degradation products were identified by mass spectroscopy and their reducing ends were all GlcA or AraA. In addition, in the photoelectrocatalytic degradation of HA, ·OH were identified as the most influential among the produced free radicals, and it could be proposed that ·OH specifically targeted the anomeric carbon of GlcA, resulting in the disaggregation of polysaccharides chain. Furthermore, the results of in vitro fermentation with fecal microbiota demonstrated that HA and its degradation products regulated microbiota structure discriminately, indicating their possible different outcomes as nutritional supplements and agents.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137813"},"PeriodicalIF":7.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0