International Journal of Biological Macromolecules最新文献_第10页

Multiscale comparative study of pectin extraction from broccoli stalk using alkaline and enzymatic modifications: Structural characterization and gelling properties. 用碱性和酶修饰从西兰花茎中提取果胶的多尺度比较研究：结构表征和胶凝特性。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-08 DOI: 10.1016/j.ijbiomac.2025.148164

Shuainan Ma, Zhixuan Yang, Haoyang Sun, Ting Wu, Siyi Pan, Xiaoyun Xu

{"title":"Multiscale comparative study of pectin extraction from broccoli stalk using alkaline and enzymatic modifications: Structural characterization and gelling properties.","authors":"Shuainan Ma, Zhixuan Yang, Haoyang Sun, Ting Wu, Siyi Pan, Xiaoyun Xu","doi":"10.1016/j.ijbiomac.2025.148164","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148164","url":null,"abstract":"To promote the application of high methoxyl pectin (HMP) extracted from broccoli stalk waste as a novel, healthy, and gelling food additive, low methoxyl pectins (LMPs) with different degrees of esterification (40-45 %, 25-30 %, and 10-15 %) were prepared from broccoli stalk pectin (BSP) using enzymatic (E-pectin) and alkaline (A-pectin) de-esterification methods. The results demonstrated that the de-esterification method and degree of esterification led to distinct structural and functional differences among the LMPs. As the degree of esterification decreased, both the molecular weight (Mw) and apparent viscosity of the LMPs gradually decreased. However, pectin obtained via enzymatic de-esterification (E-pectin) exhibited higher Mw (208.2-229.3 kDa), apparent viscosity (1.6514-8.9394 Pa·s), and thermal stability (243.9-248.5 °C) compared to pectin obtained via alkaline de-esterification (A-pectin) and commercial apple pectin (CAP). The negative charge and the proportion of rhamnogalacturonan I (RG-I) domains increased as DE decreased, with E-pectin containing a higher RG-I content (20.55-25.41 %). FTIR analysis confirmed that de-esterification treatment primarily caused the cleavage of glycosidic bonds and methoxyl groups without altering the main backbone structure of pectin, while X-ray diffraction (XRD) analysis indicated that E-pectin exhibited a lower degree of crystallinity. With decreasing DE, both E-pectin and A-pectin showed enhanced gel hardness and water-holding capacity (WHC). At comparable DE levels, E-pectin exhibited superior gel strength, WHC, and gelation temperature, with E-3 showing the highest thermal stability and gelation performance. These findings suggest that broccoli stalk-derived pectin is a promising gelling and thickening agent, with LMPs having potential for application in food products.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148164"},"PeriodicalIF":8.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characteristics of a fructan-type polysaccharide from Asparagus cochinchinensis rhizome and its anti-osteosarcoma activity mediated via macrophage polarization. 巨噬细胞极化介导的芦笋多糖结构特征及其抗骨肉瘤活性研究。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-08 DOI: 10.1016/j.ijbiomac.2025.148198

Yuxuan Xia, Wei Zong, Haiyan Lan, Jianwei Yang, Huilin Feng, Guosheng Zhao, Yixi Bao

{"title":"Structural characteristics of a fructan-type polysaccharide from Asparagus cochinchinensis rhizome and its anti-osteosarcoma activity mediated via macrophage polarization.","authors":"Yuxuan Xia, Wei Zong, Haiyan Lan, Jianwei Yang, Huilin Feng, Guosheng Zhao, Yixi Bao","doi":"10.1016/j.ijbiomac.2025.148198","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148198","url":null,"abstract":"Repolarization of tumor-associated macrophages (TAM) from the M2 to the M1 phenotype has emerged as a promising approach in cancer immunotherapy. In this study, a neutral fructan-type polysaccharide, ACRP (Asparagus cochinchinensis rhizome polysaccharide), was extracted from the rhizome of A. cochinchinensis. The molecular weights were calculated by HPGPC as Mn = 2617 Da, Mw = 3426 Da, and Mw/Mn = 1.31. Structural characterization indicated that ACRP consists of a backbone composed of β-(2 → 1)-linked Fruf and α-(1 → 6)-linked Glcp residues, terminating with T-Glcp and 2-Fruf units. In vitro assays showed that ACRP significantly activated macrophages by enhancing phagocytic potential and promoting the production of reactive oxygen species (ROS), nitric oxide (NO), TNF-α, and IL-12p70. Moreover, ACRP effectively reprogrammed M2-polarized macrophages toward the M1 phenotype, resulting in enhanced apoptosis and reduced clonogenic potential of LM8 and K7M2 osteosarcoma cells, thus indicating potent antitumor efficacy. Mechanistic studies revealed that this phenotypic transformation was mediated by activating the TLR4/MyD88/NF-κB pathway. In vivo studies using an LM8 tumor-bearing mouse model showed that ACRP modulated macrophage polarization and elevated CD3+CD4+ and CD3+CD8+ T lymphocytes, which significantly suppressed osteosarcoma progression. These findings indicate that ACRP has potent immunomodulatory and anti-tumor effects by activating innate immune responses and may represent a promising therapeutic candidate for osteosarcoma immunotherapy.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148198"},"PeriodicalIF":8.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rheology and tribology of dextran/ polyethylene oxide-based water-in-water emulsions. 葡聚糖/聚氧乙烯基水包水乳液的流变学和摩擦学研究。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-08 DOI: 10.1016/j.ijbiomac.2025.148200

Chenxi Wang, Brent S Murray, Michael G Bryant, Seunghwan Lee, Anwesha Sarkar

{"title":"Rheology and tribology of dextran/ polyethylene oxide-based water-in-water emulsions.","authors":"Chenxi Wang, Brent S Murray, Michael G Bryant, Seunghwan Lee, Anwesha Sarkar","doi":"10.1016/j.ijbiomac.2025.148200","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148200","url":null,"abstract":"This study investigated the microstructural, rheological and tribological properties of model W/W emulsions composed of dextran (D) and poly(ethylene oxide) (P) at a fundamental level. Rheological analysis revealed that increasing the P concentration, [P], resulted in increased viscosity (η), whilst increasing the D concentration, [D], intensified shear-thinning behaviour, likely due to changes in the size of D-based droplets. Confocal laser scanning microscopy (CLSM) demonstrated a significant increase in the average droplet size with higher [D] or [P]. A striking tribological result was that the W/W emulsions demonstrated an unusual speed-independent regime, with a coefficient of friction (μ) < 0.01 over a considerable range of sliding contact speed (~10 to 100 mm s-1, of physiological relevance) before the onset of the elastohydrodynamic lubrication (EHL) regime. This was not observed for solutions of the individual polymers on their own. Such composition-dependent behaviour may be due to W/W emulsion droplets entering the tribological gap, flattening and reducing the η of the entrained lubricants, thus delaying the formation of a fluid film. Overall, this detailed study shows how fabrication of W/W emulsions via phase-separating polymers can offer unique lubrication characteristics that could provide advantageous aqueous lubricants for biomedical applications.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148200"},"PeriodicalIF":8.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hybrid citrus pectin-based nanofiltration membranes incorporating meso-MIL-100(Fe) for acid-resistant and efficient dye separation 含介介- mil -100（Fe）的柑橘果胶基杂交纳滤膜耐酸高效染料分离

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-08 DOI: 10.1016/j.ijbiomac.2025.148182

Ran Song , Guiru Chen , Yunjian Chen , Hongyu Wang , Fei Wang , Luying Wang , Jiandu Lei

{"title":"Hybrid citrus pectin-based nanofiltration membranes incorporating meso-MIL-100(Fe) for acid-resistant and efficient dye separation","authors":"Ran Song , Guiru Chen , Yunjian Chen , Hongyu Wang , Fei Wang , Luying Wang , Jiandu Lei","doi":"10.1016/j.ijbiomac.2025.148182","DOIUrl":"10.1016/j.ijbiomac.2025.148182","url":null,"abstract":"<div><div>With growing demand for sustainable and biodegradable materials, the development of nanofiltration (NF) membranes from natural macromolecules has gained increasing attention. Citrus pectin (CP), an anionic polysaccharide rich in galacturonic acid, offers excellent film-forming ability, biocompatibility, and environmental friendliness. However, its poor aqueous stability and excessive swelling limit its practical applications. Ionic crosslinking with divalent and trivalent metal cations, particularly Fe3+, produced structurally robust membranes with a Congo Red (CR) rejection of 98.99 %. To further enhance performance, mesoporous MIL-100(Fe) (m-MIL) was introduced as a nanofiller. The high surface area and coordination-active Fe sites of m-MIL promoted strong molecular interactions with CP, improving compatibility and dispersion. These synergistic effects reinforced membrane integrity, permeability, and selectivity. The optimized CP/m-MIL-0.3 % membrane achieved a pure water permeability (PWP) of 153.45 LMH/bar, CR rejection of 99.62 %, and low NaCl rejection of 8.00 %. It simultaneously demonstrated excellent hydrophilicity and negative surface charge, achieving a CR/NaCl selectivity ratio as high as 131.13. This value significantly outperforms most reported natural polymer-based NF membranes. Beyond its superior separation performance, the membrane also exhibits excellent long-term stability and regeneration, with strong resistance to acid and solvents. Overall, this study demonstrates a green, scalable strategy for fabricating high-performance NF membranes with great potential for efficient and durable water treatment, particularly in acidic environments.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148182"},"PeriodicalIF":8.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering roles of Rad25, Rad3A, Rad3B and Ssl1 in UV damage recovery and insect-pathogenic lifecycle of Beauveria bassiana Rad25、Rad3A、Rad3B和Ssl1在球孢白僵菌紫外线损伤恢复和虫媒生命周期中的作用

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-08 DOI: 10.1016/j.ijbiomac.2025.148176

Min Li, Jian-Ru Feng, Sheng-Hua Ying, Ming-Guang Feng

{"title":"Deciphering roles of Rad25, Rad3A, Rad3B and Ssl1 in UV damage recovery and insect-pathogenic lifecycle of Beauveria bassiana","authors":"Min Li, Jian-Ru Feng, Sheng-Hua Ying, Ming-Guang Feng","doi":"10.1016/j.ijbiomac.2025.148176","DOIUrl":"10.1016/j.ijbiomac.2025.148176","url":null,"abstract":"<div><div>Rad25, Rad3 and Ssl1 are subunits of the transcription factor TFIIH required for nucleotide excision repair of UV-induced DNA lesions in budding yeast but remain unexplored in non-yeast fungi. Here, we report essential/similar roles of Rad25 ortholog and Rad3B (one of two Rad3 paralogs) in mediating UVB damage recovery, insect-pathogenic lifecycle and gene expression networks of Beauveria bassiana, a wide-spectrum mycoinsecticide vulnerable to solar UV damage. The recovery depended primarily on photoreactivation of UVB-impaired conidia by 3 h light plus 9 h dark incubation mimicking pre-evening daylight time plus nighttime rather than 12 h dark incubation. The photoreactivation activity arose from direct/indirect links of Rad25 and Rad3B to photolyase and photolyase regulators through multiple protein-protein interactions. Moreover, the Δrad25 and Δrad3B mutants were markedly compromised in insect pathogenicity, multiple stress tolerances, and radial growth on some carbon/nitrogen sources, and shared 2995 dysregulated genes associated with the phenotypic changes. By contrast, Rad3A or orthologous Ssl1 showed limited role in conidial thermotolerance or aerial conidiation alone. These findings unveil pleiotropic effects of Rad25 and Rad3B in the fungal lifecycle and reinforce a biophysical basis to exploit fungal anti-UV photoreactivation activity for optimal mycoinsecticide application in late afternoon on sunny summer days.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148176"},"PeriodicalIF":8.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of different gluten additions on the structure and in vitro starch digestibility of extruded quinoa noodles. 不同面筋添加量对膨化藜麦面条结构及体外淀粉消化率的影响。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-08 DOI: 10.1016/j.ijbiomac.2025.148188

Jiaxue Wang, Shengyuan Guo, Yuting Zhu, Huimin Guo, Guixing Ren

{"title":"Effect of different gluten additions on the structure and in vitro starch digestibility of extruded quinoa noodles.","authors":"Jiaxue Wang, Shengyuan Guo, Yuting Zhu, Huimin Guo, Guixing Ren","doi":"10.1016/j.ijbiomac.2025.148188","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148188","url":null,"abstract":"Quinoa is regarded as a high-quality food because of its rich nutrients and balanced amino acid. However, its natural gluten-free property limits the processing of quinoa noodles. Gluten forms a viscoelastic network, providing noodles with essential adhesion and structural stability. Thus, it is crucial to noodle quality. To resolve this issue, the study explored the effects of adding gluten at different levels (0 %, 10 %, 20 %, 30 %, and 40 %) on the structure and in vitro starch digestibility of extruded quinoa noodles. The results showed that as the proportion of gluten added increased, both the cooking and texture properties improved. This finding was further corroborated through low-field nuclear magnetic resonance and pasting properties. Fourier transform infrared spectroscopy revealed that the 1050/1022 cm-1 ratio decreased as gluten proportion increased from 0 % to 30 % but then increased at 40 %. X-ray diffraction showed a significant decrease in relative crystallinity, from 31.05 (with 0 % gluten) to 20.34 (with 40 % gluten). This indicated that the hydrogen bonds between gluten and starch molecules interfered with the short-range ordered structure of starch, and acted as a physical barrier to reduce water diffusion and starch dissolution, which confirmed the phenomenon of reduced cooking loss. In vitro starch digestibility demonstrated that with increasing proportion of gluten, the content of rapidly digestible starch decreased while the content of resistant starch increased. The addition of gluten enhanced the structure of extruded quinoa noodles, improving their hardness, elasticity, and resistant starch content, while reducing cooking loss, thereby elevating the overall product quality.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148188"},"PeriodicalIF":8.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome sequencing and comparative analysis of exopolysaccharide bioactivity from Lacticaseibacillus rhamnosus YT isolated from the feces of long-lived elderly individuals in Bama. 巴马长寿老人粪便鼠李糖乳杆菌YT全基因组测序及胞外多糖生物活性比较分析。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-08 DOI: 10.1016/j.ijbiomac.2025.148199

Peng Yu, Jianbo Su, Yiping Liu, Xin Wang, Chenchen Zhang, Dawei Chen, Yujun Huang, Ruixia Gu, Chengran Guan

{"title":"Whole genome sequencing and comparative analysis of exopolysaccharide bioactivity from Lacticaseibacillus rhamnosus YT isolated from the feces of long-lived elderly individuals in Bama.","authors":"Peng Yu, Jianbo Su, Yiping Liu, Xin Wang, Chenchen Zhang, Dawei Chen, Yujun Huang, Ruixia Gu, Chengran Guan","doi":"10.1016/j.ijbiomac.2025.148199","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148199","url":null,"abstract":"In this study, comprehensive whole-genome sequencing and comparative analysis of the exopolysaccharide (EPS) bioactivity of L. rhamnosus YT, a strain isolated from the feces of long-lived elderly individuals in Bama, Guangxi, were conducted. The genome of L. rhamnosus YT was revealed to be a circular chromosomal DNA of 2,961,987 bp, exhibiting a GC content of 46.73 % and encoding 2992 genes. Comparative genomic analysis with five other L. rhamnosus strains revealed significant collinearity in gene structure and function, yet differences were observed in gene inversions and the number of unique genes. Functional annotation demonstrated that L. rhamnosus YT possessed significant advantages in carbohydrate transport and metabolism, particularly with respect to key enzymes involved in the EPS synthesis pathway and sugar transport systems. Furthermore, a comparative assessment of the antioxidant activity, cholesterol adsorption capacity, and α-amylase inhibition activity between EPS produced by L. rhamnosus YT (EPS-YT) and L. rhamnosus GG (EPS-GG) showed that EPS-YT exhibited superior activities, especially in DPPH radical scavenging activity and cholesterol adsorption capacity. These findings provided profound insights into the genetic characteristics and adaptability of the L. rhamnosus YT, establishing a scientific foundation for its potential applications in probiotics and functional foods.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148199"},"PeriodicalIF":8.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive metabolomic and pharmacological analyses elucidate antioxidant mechanisms and flavor profiles associated with red pericarp color protein in cooked brown rice. 综合代谢组学和药理学分析阐明了熟糙米中红果皮颜色蛋白的抗氧化机制和风味特征。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-07 DOI: 10.1016/j.ijbiomac.2025.148168

Guangchen Zhang, Qiao Huang, Xiuqi Wang, Huaju Zhang, Xiaohui Zhao, Yutang He, He Liu, Jian Sun

{"title":"Comprehensive metabolomic and pharmacological analyses elucidate antioxidant mechanisms and flavor profiles associated with red pericarp color protein in cooked brown rice.","authors":"Guangchen Zhang, Qiao Huang, Xiuqi Wang, Huaju Zhang, Xiaohui Zhao, Yutang He, He Liu, Jian Sun","doi":"10.1016/j.ijbiomac.2025.148168","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148168","url":null,"abstract":"Red pericarp color (OsRc) protein is the key regulator of red seed coat pigmentation in colored rice. However, its detailed role in regulating the cooking properties of brown rice remains unclear. Here, we evaluated the antioxidant properties and analyzed the metabolite profiles of cooked brown rice. The results showed that wild-type (WT) rice exhibited significantly greater antioxidant activity than the two rc mutant rice varieties. Moreover, the total phenolic and flavonoid contents of WT rice were 2.2-fold and 5.0-fold higher than OsRc knockout. Non-volatile metabolomics analysis via Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) identified 447 differentially accumulated metabolites (DAMs). The changes in metabolite levels in phenylpropanoid, flavonoid, flavone and flavonol, and anthocyanin biosynthesis regulated by the OsRc gene may influence the antioxidant properties of brown rice. Network pharmacology and molecular docking showed that four key antioxidant metabolites, including syringetin and hesperetin, showed stable binding with key targets such as Src through hydrogen and hydrophobic bonds. Meanwhile, gas chromatography-mass spectrometry (GC-MS) identified 122 DAMs, including alcohols, esters, aldehydes, and ketones. Gas chromatography ion mobility spectrometry (GC-IMS) revealed that OsRc promoted the accumulation of heptanal, 2-propanone, isoprene, n-octanal, and 1-hexanol. These findings highlight OsRc's potential in improving the antioxidant functionality and flavor quality of brown rice.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148168"},"PeriodicalIF":8.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shedding light on a family of broad-spectrum α-hydroxy acid and polyhydroxy acid (sugar acid) isomerases. 揭示了一个广谱α-羟基酸和多羟基酸（糖酸）异构酶家族。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-07 DOI: 10.1016/j.ijbiomac.2025.148171

Julian Urdiain-Arraiza, Benoît Desguin

{"title":"Shedding light on a family of broad-spectrum α-hydroxy acid and polyhydroxy acid (sugar acid) isomerases.","authors":"Julian Urdiain-Arraiza, Benoît Desguin","doi":"10.1016/j.ijbiomac.2025.148171","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148171","url":null,"abstract":"α-Hydroxy acids (AHAs) and polyhydroxy acids (PHAs) are of key importance in organic synthesis and find numerous applications in various industries. However, the stereoselective synthesis of these compounds, whether enzymatic or chemical, remains a major challenge. In this study, we uncover a previously uncharacterized family of lactate racemase homologs (LarAHs) within the LarA superfamily and show their ability to catalyze the interconversion of AHA and PHA stereoisomers. Through systematic biochemical characterization, we investigated the substrate specificity, catalytic properties, and structural modelling of four representative LarAHs from this novel family. Our findings demonstrate that these enzymes act as broad-spectrum hydroxy acid isomerases (BSHIs) and exhibit racemase activity on up to 15 structurally diverse AHAs (including aliphatic, aromatic, and polycarboxylic AHAs), and C2-epimerase activity on up to 24 distinct PHAs (including aldonic acids, uronic acids, aldaric acids, and other sugar-derived acids). Notably, 20 of the sugar acid C2-epimerization reactions identified were previously unreported. Structural analysis revealed that BSHIs possess an original C-terminal fold that forms a substrate-binding site adapted for bulky substrates, with considerable active-site flexibility likely contributing to their broad substrate specificity. Collectively, these findings suggest the biotechnological potential of BSHIs for stereoselective interconversion of valuable AHA and PHA stereoisomers.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148171"},"PeriodicalIF":8.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and epitope characterization of anti-DEFA5 monoclonal antibodies clones 1A8 and 4F5 for inflammatory bowel disease subtype diagnostics. 抗defa5单克隆抗体克隆1A8和4F5用于炎症性肠病亚型诊断的结构和表位表征

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-07 DOI: 10.1016/j.ijbiomac.2025.148024

Rabi Thangaiyan, Anil Shanker, Billy R Ballard, Amosy E M'Koma

{"title":"Structural and epitope characterization of anti-DEFA5 monoclonal antibodies clones 1A8 and 4F5 for inflammatory bowel disease subtype diagnostics.","authors":"Rabi Thangaiyan, Anil Shanker, Billy R Ballard, Amosy E M'Koma","doi":"10.1016/j.ijbiomac.2025.148024","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148024","url":null,"abstract":"Understanding antigen-antibody interactions is key to advancing immunodiagnostics and therapeutic development. Here, we characterize two mouse monoclonal antibodies, 1A8 and 4F5, specific to human DEFA5, an innate immune effector implicated in differentiating colonic inflammatory bowel disease (IBD) subtypes, particularly Ulcerative colitis, Crohn's colitis and Indeterminate colitis. Although both antibodies target similar regions of DEFA5, they exhibit distinct binding affinities. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) revealed diffuse protection patterns with partial protection upon antibody binding, suggesting conformational stabilization in DEFA5 rather than discrete epitope recognition. Complementary peptide-based epitope mapping confirmed localized linear or semi conformational binding epitopes. Surface Plasmon Resonance (SPR) confirmed 1:1 binding stoichiometry and high-affinity binding of both antibodies for the DEFA5 epitope RATCYCRTGRCAT. Hybridoma sequencing revealed that both antibodies engage DEFA5 by an extended binding interface involving multiple complementarity-determining regions (CDRs) across both heavy and light chains, indicating structurally distributed mode of antigen recognition. Despite identical hybridoma sequencing, HADDOCK models reveal subtle binding pose differences with DEFA5 engages the VH domain in 1A8, while it interacts with the VL domain in 4F5, suggesting phenotypic variations in domain contributions. This integrative approach provides mechanistic insights into their diagnostic potential for distinguishing IBD subtypes, supporting refined tissue-based and serological assays.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148024"},"PeriodicalIF":8.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0