International Journal of Biological Macromolecules最新文献

{"title":"CdxZn1-xS nanoparticles-protein corona: Kinetics and temperature dependent interaction along with detection of the denaturation and unfolding of serum albumin","authors":"Priyanka Das ,&nbsp;Satyajit Saha ,&nbsp;Prasanta Kumar Guha ,&nbsp;Amit Kumar Bhunia","doi":"10.1016/j.ijbiomac.2025.145290","DOIUrl":"10.1016/j.ijbiomac.2025.145290","url":null,"abstract":"<div><div>In this study, the interaction and corona formation of HSA with nanostructured Cd_xZn_1-xS have been analysed through microscopic, spectroscopic and structural investigations. Binding kinetics showed corona formation time for CdS NRs, Cd_0.5Zn_0.5S NPs and ZnS NPs are 60,45,70 min, respectively. The observed time for surface reorganization (85,130,110 min) was greater than corona formation time (60, 45, 70 min, respectively). The HRTEM showed that during corona formation of HSA, CdS NRs are attached each other and Cd_0.5Zn_0.5S NPs leads aggregation of HSA through positive cooperative reaction at room temperature. Very small ZnS NPs (5-7 nm) were selectively formed protein corona on their surface and coronas were placed almost isolated way. During interaction process protein fibrillation occurs and some wrinkles were formed. The emission spectrums of nano-bio corona were studied within temperature ranges of 298–328 K, which indicated that the tertiary deformation and quenching depend on temperature with exothermic nature of bindings. The ultrafast decay showed that maximum energy transfer among HSA and CdS NRs, Cd_0.5Zn_0.5S NPs, ZnS NPs are 52.4, 37.4, 23.3 %, respectively. The unfolding of protein was occurs during interaction with gradual increment of the ratio of β-strand to α-helix of HSA from 0.13 to 4.28 due to interaction.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145290"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressure-dependent changes in physicochemical characteristics of whey protein and casein: Structure and function relationship.","authors":"Pei Zhao, Shan Zhang, Lu Xue, Tianjiao Niu, Fan Jiang, Ran Xiao, Zijian Wu","doi":"10.1016/j.ijbiomac.2025.145324","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145324","url":null,"abstract":"<p><p>As an emerging non-thermal processing technology, Ultra-high-pressure jet (UHPJ) processing has the effect of homogenizing and sterilizing materials concurrently. The UHPJ-treated skimmed milk was employed in this study to reveal the impact of pressure as the primary factor on protein structure and physicochemical properties. The results showed that with the increase of jet pressure, whey protein's endogenous fluorescence intensity increases, and casein's exogenous fluorescence intensity increases. The structure of casein tends to be loose, in contrast to the aggregation tendency of whey protein. After pressure treatment at 100-200 MPa, the thiol content peaked at 0.11 μmol/g. The content of α-helix increased to 17 %. The emulsifying stability of skim milk improved to 18.48 m²/g, and its foaming expansion index and foaming stability index attained values of 27.65 % and 24.9 %, respectively. The digestion rate of milk proteins processed at 200 MPa was the highest in the gastric digestion stage and relatively high in the intestinal digestion stage. At 250 and 300 MPa, the SDS - PAGE electrophoresis results showed that the loose binding of casein and 18 kDa β-Lactoglobulin under high pressure was separated. The instantaneous decompression characteristic of UHPJ tends to destabilize the emulsion system by disrupting the formed interfacial layer, making the 250 and 300 MPa pressure range unfavorable for emulsion stabilization. This study will provide a scientific basis for applying UHPJ in the dairy industry.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145324"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening efficacy of spherical starch nanoparticles and surrounding interphase in polymer nanocomposites.","authors":"Yasser Zare, Muhammad Naqvi, Kyong Yop Rhee, Soo-Jin Park","doi":"10.1016/j.ijbiomac.2025.145317","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145317","url":null,"abstract":"<p><p>Although extensive experimental data exist, modeling studies on the tensile strength of starch-filled nanocomposites remain incomplete, hindering the optimization of formulations. In this study, the Nicolais-Narkis model is modified and expanded by introducing the interfacial parameter a, enabling the estimation of tensile strength in starch-based nanocomposites. The enhanced model incorporates critical factors such as interphase thickness, interphase strength, and starch particle size. Experimental data from various starch-filled samples are utilized to validate the proposed model. Furthermore, parametric analyses are conducted to evaluate the influence of all relevant parameters on the interfacial parameter a and the overall strength of the nanocomposites. The results indicate that a starch radius (R) of 20 nm with an interphase depth (t) of 50 nm yields interface parameter (a) of 10, resulting in a 300 % improvement in nanocomposite strength. In contrast, R = 90 nm and t = 15 nm result in a < 0, offering no reinforcement. These findings underscore that smaller nanoparticles and a denser interphase significantly enhance nanocomposite strength. Conversely, larger nanoparticles and a thinner interphase fail to improve the mechanical properties. Additionally, the highest values of a and nanocomposite strength are achieved with the weakest polymer matrix and the most robust interphase. In contrast, a strong polymer matrix and a weak interphase lead to a < 0, resulting in no reinforcement.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145317"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the dissolution and degradation patterns of cellulose in phosphonate-based ionic liquids and the construction of ternary phase diagrams.","authors":"Jing Tian, Hongshuai Gao, Yan Long, Chenguang Wang, Binqi Wang, Ruimei Cao, Yi Nie","doi":"10.1016/j.ijbiomac.2025.145321","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145321","url":null,"abstract":"<p><p>The growing demand for sustainable materials necessitates green solvents for cellulose fiber production. This study addresses the unclear mechanisms of phosphonate-based ionic liquids (ILs) in cellulose dissolution and regeneration, which limit their industrial application. Five phosphonate-based ILs were synthesized and characterized: 1-butyl-3-methylimidazolium methylphosphonate ([Bmim]MP), 1-ethyl-3-methylimidazolium dimethylphosphate ([Emim]DMP), 1-ethyl-3-methylimidazolium ethylphosphonate ([Emim]EP), 1-ethyl-3-methylimidazolium methylphosphonate ([Emim]MP), and 1,3-dimethylimidazolium methylphosphonate ([Mmim]MP). Polarized light microscopy images demonstrate that cellulose can be dissolved in these ILs at 60 °C. Molecular weight analysis revealed that degradation patterns are influenced by both time and temperature, and the extent of degradation was objectively ranked according to measured molecular weight changes. The strongest hydrogen bonding of [Emim]MP to cellulose was calculated using density functional theory, with a bond strength of 124.24 kJ/mol. The ternary phase diagram obtained from turbidimetric titration visualizes the effect of regeneration conditions on the process, where ion size and chain length influence the outcomes. Furthermore, the regeneration capabilities of each ternary system were validated by determining excess enthalpy during regeneration using the COSMO-RS method. This study provides data and theoretical references for applying phosphonate-based ILs in the spinning process.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145321"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling new Kv1.3 channel blockers from scorpion venom: Characterization of Meuk7-3 and in silico design of its analogs for enhanced affinity and therapeutic potential.","authors":"Saeedeh Shariati, Ladan Mafakher, Maryam Shirani, Masoumeh Baradaran","doi":"10.1016/j.ijbiomac.2025.145327","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145327","url":null,"abstract":"<p><p>Kv1.3 channels are associated with autoimmune and neuroinflammatory diseases. Scorpion venom is an excellent source of inhibitors for Kv1.3. Kv1.3, a pivotal voltage-gated potassium channel, has emerged as a critical therapeutic target for combating autoimmune and neuroinflammatory diseases, including multiple sclerosis and rheumatoid arthritis. Some studies have tried to discover highly selective toxins targeting Kv1.3 channels, but it is still challenging. Here, we present a groundbreaking discovery of a potent peptide potassium channel blocker, Meuk7-3, derived from the venom of the scorpion, Mesobuthus crucittii. While similar to other Kv1.3 blockers, Meuk7-3's Lys19 residue may enhance its affinity for the channel. So, we redesigned the Meuk7-3 and generated three analogous, Meuk7-3 A, Meuk7-3B, and Meuk7-3C, to improve its drug-like properties and affinity to Kv1.3. Interaction evaluation with Kv1.3 revealed that Meuk7-3 and all its designed analogous could of Kv1.3's pore through the interaction of Lys19 of the peptide with Tyr447, Tyr797, Tyr1147, and Tyr1497, critical residues located at the channel pore of Kv1.3.However, the stability of the interaction of designed peptides with Kv1.3 was more than Meuk7-3. Binding affinity analysis revealed that all designed peptides had a better binding affinity to Kv1.3 than Meuk7-3. Among the three analogous, Meuk7-3 A was found to have better drug-like properties and interaction situations, including binding energy and affinity to Kv1.3, compared to Meuk-3 native. These findings provide new data for designing highly effective Kv1.3 inhibitors by computational tools for treating autoimmune and inflammatory diseases, although experimental testing is necessary to validate them.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145327"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Types, properties, and applications of polysaccharide-based wound dressings: A review","authors":"Hongkun Xue ,&nbsp;Zhangmeng Shi ,&nbsp;Lihong Ji ,&nbsp;Jiacheng Yang ,&nbsp;Hao Yu ,&nbsp;Jiaqi Tan","doi":"10.1016/j.ijbiomac.2025.145237","DOIUrl":"10.1016/j.ijbiomac.2025.145237","url":null,"abstract":"<div><div>Wound management is a major challenge worldwide, imposing a substantial financial burden on the governments of every country. In recent years, numerous studies have focused on wound dressings that promoted wound healing. Notably, traditional wound dressings (tourniquets, gauze, and bandages) do not respond well to the wound healing process, resulting in poor wound healing. Polysaccharide-based materials (chitosan, sodium alginate, pectin, starch, and cellulose, etc.) are expected to be developed as potential wound dressings due to their low toxicity, good biodegradability, biocompatibility, adhesion, and loading properties. Growing studies have confirmed that polysaccharide-based wound dressings (PWDs) could provide a moist healing environment, promote wound healing, and reduce the risk of infection. An in-depth exploration of the physicochemical and functional properties of PWDs holds significant guiding importance for the designing of PWDs with specific functionalities. Hence, this review systematically introduces the PWDs types. Moreover, this paper comprehensively reviews the physicochemical and functional properties of PWDs. Furthermore, this review also outlines the application of PWDs for different types of wounds. The findings provide valuable insights and guidance for the further development and utilization of PWDs.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"319 ","pages":"Article 145237"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined cellulose acetate butyrate and palmitic acid solvent exchange-driven phase inversion in situ gel loading moxifloxacin HCl for periodontitis therapy","authors":"Warakon Thammasut ,&nbsp;Wantanwa Krongrawa ,&nbsp;Catleya Rojviriya ,&nbsp;Pisit Pitchayakorn ,&nbsp;Thawatchai Phaechamud","doi":"10.1016/j.ijbiomac.2025.145314","DOIUrl":"10.1016/j.ijbiomac.2025.145314","url":null,"abstract":"<div><div>This study develops in situ forming gels (ISGs) incorporating cellulose acetate butyrate (CAB) and palmitic acid (PAL) as matrix-forming agents for localized periodontal drug delivery. The aim is to investigate the effects of CAB, PAL, and their combination on viscosity, rheological behavior, matrix formation, drug release, structural properties, and antimicrobial efficacy of moxifloxacin (Mx)-loaded ISGs. Drug release was examined, and release kinetics were determined using mathematical modeling. Scanning electron microscopy (SEM) and X-ray tomographic microscopy (XTM) were used to characterize matrix structures. Antimicrobial activity was assessed against <em>Staphylococcus aureus</em>, <em>Candida albicans</em>, and <em>Porphyromonas gingivalis</em> over 14 days using an agar diffusion assay. CAB dominant ISGs formed dense, slow-releasing matrices, while PAL dominant ISGs exhibited rapid phase inversion due to swift solvent exchange and higher porosity. NMP-based ISGs formed more porous matrices than DMSO-based ISGs in PAL-dominant formulations, enhancing drug diffusion at later stages. CAB/PAL combination ISGs balanced injectability, phase inversion, and prolonged drug release. Antimicrobial studies also confirmed sustained activity, with CAB dominant formulations maintaining efficacy for 14 days. CAB/PAL-based ISGs offer a promising strategy for sustained periodontal drug delivery while solvent selection modulates porosity and antimicrobial activity. This system demonstrates potential for prolonged therapeutic efficacy in localized periodontal treatment.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145314"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted delivery of doxorubicin toward 4 T1 cells via in situ binding between maleimide functionalized AS1411 − NH2 aptamer and endogenous albumin","authors":"Yasamin Hamooni ,&nbsp;Ali Samie ,&nbsp;Mona Alibolandi ,&nbsp;Mohammad Ramezani ,&nbsp;Khalil Abnous ,&nbsp;Seyed Mohammad Taghdisi","doi":"10.1016/j.ijbiomac.2025.145236","DOIUrl":"10.1016/j.ijbiomac.2025.145236","url":null,"abstract":"<div><div>Trojan horse strategy is a targeted delivery system that directs therapeutic agents toward specific sites through <em>in situ</em> binding with circulating endogenous albumin. This study developed a Trojan horse system utilizing AS1411 − NH₂ aptamer (Apt), Mal-PEG_3.5-NHS ester (PEG), human/mouse serum albumin (HSA/MSA), and doxorubicin (DOX). The formation of Apt−PEG conjugate was confirmed <em>via</em> agarose gel electrophoresis, while its attachment to HSA was validated using SDS-PAGE. The loading capacity for transporting DOX was assessed by quenching the fluorescence intensity of DOX. The PEG's maleimide formed an <em>in situ</em> bond with albumin's cysteine-34, producing DOX − Apt−PEG−HSA compound <em>in vitro</em>/DOX − Apt−PEG−MSA complex <em>in vivo</em>. The DLS revealed proper size and negative charge distribution, and the drug release profile demonstrated stability and pH-sensitivity of the compound. The functionality and internalization of DOX − Apt−PEG composite were validated through <em>in vitro</em> assays, including MTT, live/dead, flow cytometry, apoptosis/necrosis, and fluorescence imaging. <em>In vivo</em> assessments of DOX within the complex on six 4 T1 tumor-bearing mice presented four cases of tumor shrinkage and two cases of tumor ablation. Histological studies and <em>ex vivo</em>/<em>in vivo</em> fluorescence imaging on tumor-bearing mice exhibited significant accumulation of complex, leading to massive tumor necrosis and less accumulation in other organs, validating a noticeable reduction in overall toxicity.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145236"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cullin4-Ring ligase-mediated filamenting temperature-sensitive Z 2 homeostasis affects plastid level and fruit quality in tomato","authors":"Hongtao Wang ,&nbsp;Binglong Li ,&nbsp;Min Miao ,&nbsp;Tao Zhang ,&nbsp;Haodi Wang ,&nbsp;Xu Wang ,&nbsp;Shilong Jia ,&nbsp;Songhu Wang ,&nbsp;Pengpeng Zheng ,&nbsp;Yongsheng Liu ,&nbsp;Xiaofeng Tang ,&nbsp;Lihuan Wang","doi":"10.1016/j.ijbiomac.2025.145287","DOIUrl":"10.1016/j.ijbiomac.2025.145287","url":null,"abstract":"<div><div>Plants have evolved intricate regulatory mechanisms to balance growth and defense. In particular, the UV-damaged DNA-binding protein 1 (DDB1), which can form an E3 ubiquitin ligase with CUL4 (Cullin4-Ring Ligase, CRL4), is widely involved in plant growth and response to adversity. The tomato spontaneous <em>SlDDB1</em>-defective <em>high pigment 1</em> (<em>hp1</em>) mutant manifests significantly increased plastid level and pigments accumulation, but the underlying molecular mechanism remains unclear. Here we report the isolation and characterization of two novel SlDDB1-interacting proteins, SlFtsZ2–1 and SlFtsZ2–2, identified by a yeast two-hybrid assay. They showed constitutive expression patterns and chloroplast localizations. CRISPR/Cas9 knockout uncovered a functional redundancy between SlFtsZ2–1 and SlFtsZ2–2 since only their double knockout mutant displayed significantly decreased plastid level and fruit nutrient accumulation. The interactions between the SlDDB1/SlCUL4 and SlFtsZ2–1/SlFtsZ2–2 within chloroplasts were subsequently validated through co-immunoprecipitation and fluorescence-based assays. Furthermore, biochemical and molecular analyses demonstrated that both SlFtsZ2–1/SlFtsZ2–2 proteins are targeted for ubiquitination and degradation by the CRL4 E3 ligase complex, uncovering a previously unknown role of CRL4 in plastid proteostasis. Collectively, our findings elucidate a novel regulatory module, SlCUL4-SlDDB1-SlFtsZ2, which is distinct from the existing chloroplast-associated protein degradation (CHLORAD) pathway. This module plays a pivotal role in the precise control of SlFtsZ2 protein homeostasis, thereby influencing the plastid level and fruit quality in tomato. This study provides a mechanistic foundation for improving crop nutrient content through ubiquitination pathway manipulation and indicates potential agricultural applications in fruit quality regulation.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145287"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carboxymethylcellulose-fucoidan dissolvable microneedle patches for minimally invasive melanoma treatment: Demonstration on a 3D bioprinted A375 cell line model.","authors":"Ana C Q Silva, Maria C Teixeira, Ana Jesus, Paulo C Costa, Isabel F Almeida, Patrícia Dias-Pereira, Inês Correia-Sá, Helena Oliveira, Armando J D Silvestre, Carla Vilela, Carmen S R Freire","doi":"10.1016/j.ijbiomac.2025.145320","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145320","url":null,"abstract":"<p><p>Melanoma often requires adjuvant therapy to combat tumor proliferation and metastasis. In this context, microneedle systems (MNs) present a promising avenue for minimally invasive delivery of drugs or bioactive compounds with natural anticancer properties, targeting the deeper layers of the skin. Herein, we describe the fabrication of bioactive dissolving microneedles composed of carboxymethylcellulose (CMC) and fucoidan (Fuc) using a simple and eco-friendly micromolding technique. The microneedles showcased integral bodies and sharp tips with heights of 456 μm, and robust mechanical properties, reaching a maximum force of 1.07 N needle^-1. Preliminary insertion tests in a polymeric skin model demonstrated the ability of CMC_Fuc MNs to penetrate up to 381 μm, further validated in ex vivo human skin samples with insertion depths of 325-453 μm. Dissolution studies in an agarose hydrogel skin model revealed the complete dissolution of the MNs tips within 12 min. In vitro cytotoxicity assays unveiled the antitumoral effect of the CMC_Fuc MNs on A375 melanoma cells, leading to a significant cell viability reduction in both a 2D cell culture (ca. 83 %) and a 3D bioprinted melanoma culture model (ca. 56 %), after 48 h. The CMC_Fuc microneedle systems show great promise for minimally invasive adjuvant treatment of melanoma.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145320"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}