International Journal of Biological Macromolecules最新文献_第10页

Insights into the interaction of tris (2,4-di-tert-butyl phenyl) phosphite (I 168) and its metabolites (24DP) with pepsin: Enzyme inhibition, structural impacts, and antioxidant interference 三（2,4-二叔丁基苯基）亚磷酸酯（I 168）及其代谢产物（24DP）与胃蛋白酶的相互作用：酶抑制、结构影响和抗氧化干扰

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146442

Wu Lijuan , Li Jiayin , Yang Yin , Ju Kaize , Jia Ziyao , Gao Biqiang , Jin Shiwei , Sun Qun , Wu Laiyan

{"title":"Insights into the interaction of tris (2,4-di-tert-butyl phenyl) phosphite (I 168) and its metabolites (24DP) with pepsin: Enzyme inhibition, structural impacts, and antioxidant interference","authors":"Wu Lijuan , Li Jiayin , Yang Yin , Ju Kaize , Jia Ziyao , Gao Biqiang , Jin Shiwei , Sun Qun , Wu Laiyan","doi":"10.1016/j.ijbiomac.2025.146442","DOIUrl":"10.1016/j.ijbiomac.2025.146442","url":null,"abstract":"<div><div>This study investigated the interactions between pepsin (PEP) and the organophosphite antioxidant Irgafos 168 (I 168), its metabolite 2,4-di-tert-butylphenol (24DP), focusing on structural, functional, and antioxidant implications. UV–Vis absorption spectra, fluorescence lifetimes and Stern-Volmer quenching analyses show that I 168/24DP statically bursts PEP with high binding affinity. Thermodynamic parameters, molecular docking analyses and molecular dynamics simulations (e.g. RMSD, Rg) indicate that the spontaneous formation of the stable complexes is mainly driven by van der Waals forces and hydrophobic interactions. I 168 exhibited higher pepsin inhibition (6.97 %–27.51 %) than 24DP (5.04 %–19.29 %), with dose-dependent suppression linked to altered catalytic residue accessibility (e.g., Asp32/Asp215, Tyr75) and disrupted proton transfer efficiency. Structural analyses revealed I 168 increased PEP's flexibility and hydrophobic residue exposure, while 24DP enhanced rigidity and compactness. Antioxidant assays showed 24DP's superior DPPH scavenging capacity (9.45 %–47.35 %) compared to I 168 (2.28 %–8.00 %), though PEP significantly suppressed 24DP's antioxidant efficacy. These findings underscore the necessity of evaluating both parent compounds and metabolites in safety assessments, as metabolic processes retained biological impacts on enzymatic and antioxidant functions. The study provides critical insights into the molecular mechanisms of antioxidant-induced enzyme inhibition and highlights risks to digestive health and food safety.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146442"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Deposition of alginate-oregano nanofibres on cotton gauze for potential antimicrobial applications" [Int. J. Biol. Macromol. Vol. 319 (2025), 1-16 Article 145, 372]. “海藻酸盐-牛至叶纳米纤维在棉纱上沉积的潜在抗菌应用”的勘误表[Int.]。生物。絮凝。第319卷（2025），1-16条[j]。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146240

Abimbola Oluwatayo Orisawayi, Hao Lu, Ishrat Jahan Badruddin, Prabhuraj D Venkatraman, Nicole S Britten, Jonathan A Butler, Krzysztof Koziol, Sameer S Rahatekar

引用次数: 0

Multifunctional chitosan-based bioactive films incorporated with Jaboticaba pomace extract for sustainable pork preservation. 以壳聚糖为基础的多功能生物活性膜与大豆渣提取物的可持续猪肉保存。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146439

Ying-Chen Chen, Chih-Huang Weng, Shang-Ming Huang, Girma Sisay Wolde, Jenn-Wen Huang, Chanat Chokejaroenrat, Pattanateeradetch Apiladda, Yao-Tung Lin

{"title":"Multifunctional chitosan-based bioactive films incorporated with Jaboticaba pomace extract for sustainable pork preservation.","authors":"Ying-Chen Chen, Chih-Huang Weng, Shang-Ming Huang, Girma Sisay Wolde, Jenn-Wen Huang, Chanat Chokejaroenrat, Pattanateeradetch Apiladda, Yao-Tung Lin","doi":"10.1016/j.ijbiomac.2025.146439","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.146439","url":null,"abstract":"<p><p>Valorizing food industry by-products is essential for sustainable food packaging. This study develops a bioactive film composed of waste shrimp shell-derived chitosan (CTS) and jaboticaba pomace extract (JAE) to enhance the preservation of fresh pork by leveraging the natural bioactivity of both components. Incorporating JAE significantly enhanced the antimicrobial ability (5.7-log-inactivation of E. coli within 720 min), antioxidant capacity, UV-blocking, and mechanical properties of CTS film, making them a superior alternative to conventional packaging. Fresh pork loins wrapped in CTS-JAE film maintained quality for 12 days at 4 °C, with pH (5.5), total volatile basic nitrogen (TVBN, 10.3 mg/100 g), thiobarbituric acid reactive substances (TBARS, 0.16 mg/kg), and total bacterial count (3.7 log CFU/g) remaining within acceptable limits. Structural analyses using scanning electron microscopy (SEM) and atomic force microscopy (AFM) revealed bacterial membrane disruption as the primary antimicrobial mechanism, further validating the film's effectiveness in controlling microbial growth. Moreover, the CTS-JAE films' strong UV-blocking properties and enhanced mechanical stability contribute to their potential as a multifunctional packaging solution. These findings demonstrate CTS-JAE films as a sustainable alternative to plastic packaging, aligning with circular economy principles and advancing next-generation food packaging.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"146439"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longan polysaccharides promote Th1 and Treg cell differentiation via dendritic cells 龙眼多糖通过树突状细胞促进Th1和Treg细胞分化

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146462

Shengwei Wang , Xiaoyan Chen , Qianxin Li , Yanxian Feng , Qianming Yang , Ruili Yang , Wu Li

{"title":"Longan polysaccharides promote Th1 and Treg cell differentiation via dendritic cells","authors":"Shengwei Wang , Xiaoyan Chen , Qianxin Li , Yanxian Feng , Qianming Yang , Ruili Yang , Wu Li","doi":"10.1016/j.ijbiomac.2025.146462","DOIUrl":"10.1016/j.ijbiomac.2025.146462","url":null,"abstract":"<div><div>In this study, the activating effects of a glucan derived from longan (LP) on primary bone marrow dendritic cells (BMDCs) and their role in mediating T cells differentiation were investigated. LP significantly reduce phagocytosis in dendritic cells (DCs) while augmenting CD80, CD86, and MHC II surface molecule expression, and promoting the IL-6, IL-10, and IL-12 secretion. RNA-seq, immunofluorescence, and Western blot analysis indicated that LP primarily activate DC through the Toll-NF-κB pathway, upregulating genes related to maturation and antigen presentation, thereby regulating cytokine release and T cell activation. In addition, LP promote the CD3+ T cells proliferation and increase the CD3+ CD4+ and CD3+ CD8+ T cells ratio through DC mediation. Notably, LP increase the proportion of CD3+ CD4+ IFN-γ+ Th1 cells and CD3+ CD4+ CD25+ Foxp3+ T regulatory cells, while concurrently decreasing CD3+ CD4+ IL-4+ Th2 cells. These results indicate that LP promotes T cell differentiation towards Th1 and Treg subsets through dendritic cell mediation, enhancing a Th1-dominated cellular immune response alongside Treg-mediated immune regulation.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146462"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coordinated dysfunction in oxidative phosphorylation–related proteins and a delayed acrosome reaction during sperm capacitation reduces male fertility 氧化磷酸化相关蛋白的协同功能障碍和精子获能过程中顶体反应的延迟降低了男性的生育能力

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146349

Yoo-Jin Park, Gangaraju Gedda, Myung-Geol Pang

{"title":"Coordinated dysfunction in oxidative phosphorylation–related proteins and a delayed acrosome reaction during sperm capacitation reduces male fertility","authors":"Yoo-Jin Park, Gangaraju Gedda, Myung-Geol Pang","doi":"10.1016/j.ijbiomac.2025.146349","DOIUrl":"10.1016/j.ijbiomac.2025.146349","url":null,"abstract":"<div><div>Although dynamic changes of proteomes during sperm capacitation are well documented by the advanced proteomic research, spatiotemporal changed proteins during capacitation according to sperm fertility remain elusive. This study aimed to investigate temporal protein changes in spermatozoa during capacitation in relation to fertility status. Initially, proteomic analysis was conducted on spermatozoa at early (20 min) and late (120 min) stages of capacitation to comprehensively identify protein modifications during capacitation, irrespective of fertility status (n = 6). After late capacitation, a decrease in acrosome vesicle-associated proteins and an increase in oxidative phosphorylation (OXPHOS)-related proteins were observed. To determine whether these temporal protein changes are linked to fertility status, western blot were conducted using normal fertility (NF, n = 5) and below-normal fertility (BNF, n = 5) bulls during capacitation. We found that mitochondrial respiratory complex proteins II–IV were abundant in BNF spermatozoa before and during capacitation. During capacitation, NF sperm showed gradual acrosomal protein degradation, while BNF sperm retained intact proteins and showed higher ROS accumulation in the acrosome after late capacitation. These results suggest that systemic instability in OXPHOS may promote ROS accumulation in the acrosome during late capacitation, potentially delaying the acrosome reaction and thereby reducing fertility.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146349"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the colonic release and serum glucose regulation of insulin by co-loaded a prebiotic in alginate composite microcapsules 海藻酸盐复合微胶囊共载益生元改善胰岛素的结肠释放和血糖调节

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146441

Yuan-Bao Jin , Yun-Shan Wei , Yi-Xi Yang , Kun Feng , Hong Wu

{"title":"Improving the colonic release and serum glucose regulation of insulin by co-loaded a prebiotic in alginate composite microcapsules","authors":"Yuan-Bao Jin , Yun-Shan Wei , Yi-Xi Yang , Kun Feng , Hong Wu","doi":"10.1016/j.ijbiomac.2025.146441","DOIUrl":"10.1016/j.ijbiomac.2025.146441","url":null,"abstract":"<div><div>Oral insulin (INS) administration can considerably enhance compliance of diabetes patients. The polysaccharides-based microbiota-triggered colon delivery system is considered to have the most potential, but successful release of INS is limited due to intestinal microbiota disturbances in diabetic patients. Therefore, this study attempts to construct a INS colon delivery system that can collaboratively improve intestinal microbiota. Firstly, a prebiotic/INS co-loaded microcapsules (FIPM) was constructed through coaxial electrospraying. Then, ES100 coating was processed and successfully reduced INS premature release from the obtained system (EFIPM) to <20 %. Moreover, due to presence of prebiotic in EFIPM, the intestinal flora diversity of diabetic mice was significantly enhanced, which was conducive to the efficient release of INS in the colon within 4 h. Finally<em>,</em> results of in vivo experiments showed that diabetic mice with EFIPM intervention have more beneficial pharmacokinetic and pharmacological markers. Specifically, the blood glucose level in the EFIPM group was consistently maintained at approximately 20 % lower than EIPM (INS only loaded) intervention group. The pharmacological bioavailability of INS increased by 50 % (35.6 % vs. 23.9 %). In conclusion, these results suggest that intestinal microbiota diversity is closely related to oral INS bioavailability, and the developed EFIPM is potential in INS oral delivery.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146441"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular valorization of Hypericum perforatum biomass into CuO-lignin and CuO-cellulose bionanocomposites for mitigating copper nanotoxicity in plants 贯叶连翘（Hypericum perforatum）生物质循环转化为cuo -木质素和cuo -纤维素生物纳米复合材料以减轻植物中的铜纳米毒性。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146474

Megha Saxena , Lakshmipathy Muthukrishnan , Matam Pradeep , Rajendran K. Selvakesavan , Gregory Franklin , Dibyendu Mondal

{"title":"Circular valorization of Hypericum perforatum biomass into CuO-lignin and CuO-cellulose bionanocomposites for mitigating copper nanotoxicity in plants","authors":"Megha Saxena , Lakshmipathy Muthukrishnan , Matam Pradeep , Rajendran K. Selvakesavan , Gregory Franklin , Dibyendu Mondal","doi":"10.1016/j.ijbiomac.2025.146474","DOIUrl":"10.1016/j.ijbiomac.2025.146474","url":null,"abstract":"<div><div>We present a circular biomass valorisation strategy for the synthesis of CuO-based nanocomposites (NCs) using <em>Hypericum perforatum</em> L. In this approach, <em>H. perforatum</em> serves a dual purpose: its phytochemicals are used to synthesize CuO nanoparticles (NPs), while the remaining biomass is used to extract lignin and cellulose. The extracted lignin and cellulose were then processed into lignin nanoparticles (LNPs) and cellulose nanofibers (CNFs). These were subsequently functionalized with CuO NPs to fabricate CuO-LNPs NC and CuO-CNFs NC. Characterization using UV–Vis, FTIR, DLS, pXRD, and UPLC-PDA confirmed the successful formation of all nanocomposites. Microscopy analysis showed CuO NPs encapsulated within spherical LNPs and embedded in a net-like structure formed by CNFs. The release of Cu ions measured by TXRF over 15 days showed that CuO-LNPs release Cu ions significantly slower than CuO NPs due to the strong interactions between CuO and LNPs. In contrast, CuO-CNFs NC exhibited similar release rates to CuO NPs, attributed to weaker binding. In vitro testing on tobacco BY-2 cells corroborated with slow release profile. CuO NPs and CuO-CNFs NC, which reduced cell viability after 7 days, CuO-LNPs NC maintained plant cell viability and showed no cytotoxicity. Overall, this integrated approach increases the value of biomass for the production of high-value nanomaterials that promotes environmental and economic sustainability, while envisaging CuO-LNPs NC as a potential slow-release nanomaterial for sustainable agriculture.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146474"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building of zinc borate@bamboo cellulose composite aerogels with excellent acoustic performance and thermal insulation inspired by breakwater. 建筑锌borate@bamboo纤维素复合气凝胶具有优异的声学性能和隔热防波堤的灵感。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146476

Jiatong Yan, Shan Jiang, Chuanxi Lin, Weijie Wang, Li Liu, Hong Tang, Ronghui Guo

{"title":"Building of zinc borate@bamboo cellulose composite aerogels with excellent acoustic performance and thermal insulation inspired by breakwater.","authors":"Jiatong Yan, Shan Jiang, Chuanxi Lin, Weijie Wang, Li Liu, Hong Tang, Ronghui Guo","doi":"10.1016/j.ijbiomac.2025.146476","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.146476","url":null,"abstract":"<p><p>The noise pollution and climate warming have attracted more and more attention due to the rapid development of urbanization and industry. The preparation of lightweight aerogels with excellent acoustic performance, thermal insulation, and flame retardancy can quickly adapt to practical environments. Inspired by the breakwater, ZB@BC aerogels were successfully synthesized by sol-gel process, freeze-drying, and adsorption and precipitation. Noise reduction coefficient (NRC) value of ZB@BC composite aerogels is 0.48 and average sound absorption coefficient is 0.72 in 800-6300 Hz. Average transmission loss is 30.31 dB in 50-6300 Hz when the volume of sound source is 85 dB. In addition, ZB@BC composite aerogels exhibit excellent thermal conductivity (0.053 W/mK at 25 °C). The surface temperature is only 42.1 °C when the ZB@BC aerogel is placed on hot stage (100 °C), which proves its thermal insulation performance. Specifically, ZB@BC composite aerogels are flame-retardant with the limit oxygen index (LOI) value of 30.6 %. ZB@BC composite aerogels show compressive strength of 0.28 MPa and Young's modulus of 2.79 MPa. ZB@BC composite aerogels exhibit the advantages of low density, excellent acoustic performance, thermal insulation, flame retardancy, and mechanical strength compared with commercial acoustic and thermal insulation materials.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"146476"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lentinan attenuates histone deacetylation of SOCS1 promoter to remodel autophagy via JAK2/STAT3 pathway for the prevention of intervertebral disc degeneration. 香菇多糖减弱SOCS1启动子的组蛋白去乙酰化，通过JAK2/STAT3途径重塑自噬，预防椎间盘退变。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146457

Kangle Song, Hanwen Gu, Qi Li, Xiangzhen Kong, Yanlin Li, Qunbo Meng, Kaiwen Liu, Zhenchuan Liu, Zexin Wang, Yuanqiang Zhang, Qing Xie, Lei Cheng

{"title":"Lentinan attenuates histone deacetylation of SOCS1 promoter to remodel autophagy via JAK2/STAT3 pathway for the prevention of intervertebral disc degeneration.","authors":"Kangle Song, Hanwen Gu, Qi Li, Xiangzhen Kong, Yanlin Li, Qunbo Meng, Kaiwen Liu, Zhenchuan Liu, Zexin Wang, Yuanqiang Zhang, Qing Xie, Lei Cheng","doi":"10.1016/j.ijbiomac.2025.146457","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.146457","url":null,"abstract":"<p><p>Intervertebral disc degeneration (IVDD), driven by nucleus pulposus cell (NPCs) apoptosis, extracellular matrix (ECM) dyshomeostasis, and inflammation, lacks effective therapies. Lentinan (LNT), a polysaccharide from lentinula edodes, has anti-inflammatory and antioxidant properties. However, the role and molecular mechanisms of LNT in IVDD are unclear. In vitro, LNT counteracted IL-1β-induced NPCs apoptosis by restoring mitochondrial function, suppressing reactive oxygen species (ROS) accumulation, and rebalancing ECM metabolism via regulation of anabolic and catabolic markers. Mechanistic studies revealed that LNT enhanced autophagic flux by upregulating Suppressor of Cytokine Signaling 1 (SOCS1) expression, which inhibited Janus Kinase2 (JAK2) / Signal Transducer and Activator of Transcription 3 (STAT3) hyperactivation, thereby alleviating NPCs senescence and apoptosis under inflammation. Specifically, LNT downregulates histone deacetylase 6 (HDAC6), enhancing histone H3 lysine 27 acetylation (H3K27ac). This epigenetic modification promotes SOCS1 enhanced expression, which in turn suppresses the JAK2/STAT3 pathway, ultimately alleviating intervertebral disc degeneration. In vivo, LNT administration in a rat caudal IVDD model attenuated disc degeneration, as evidenced by preserved disc morphology, reduced ECM degradation. Immunohistochemical (IHC) and immunofluorescence (IF) experiments demonstrated that lentinan mediates the regulation of SOCS1 and restores the impaired autophagy status in rat tail intervertebral discs following puncture-induced injury. In human nucleus pulposus specimens, we have also identified the involvement of SOCS1/JAK2/STAT3 signaling in aged and degenerated nucleus pulposus. Consequently, LNT may be a promising candidate for the treatment of IVDD.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"146457"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NDRG2 regulates milk fat biosynthesis by targeting the AMPK/SREBP1 axis in bovine mammary epithelial cells NDRG2通过靶向牛乳腺上皮细胞AMPK/SREBP1轴调控乳脂合成。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-07-30 DOI: 10.1016/j.ijbiomac.2025.146448

Ji Cheng , LiJun Wang , Lulu Li , Tinghong Kuang , Haiyan Su , Keyan Ren , Shifeng Pan

{"title":"NDRG2 regulates milk fat biosynthesis by targeting the AMPK/SREBP1 axis in bovine mammary epithelial cells","authors":"Ji Cheng , LiJun Wang , Lulu Li , Tinghong Kuang , Haiyan Su , Keyan Ren , Shifeng Pan","doi":"10.1016/j.ijbiomac.2025.146448","DOIUrl":"10.1016/j.ijbiomac.2025.146448","url":null,"abstract":"<div><div>Milk fat serves as the primary energy source for both humans and animals, playing a pivotal role in various metabolic and physiological processes. N-myc downstream regulated gene 2 (NDRG2), a member of the NDRG family, is closely involved in cellular proliferation and differentiation. However, the specific role of NDRG2 in milk fat synthesis within bovine mammary epithelial cells (BMECs), as well as its underlying molecular mechanisms, remains unclear. RNA sequencing of BMECs from low-fat and high-fat dairy cows revealed 247 up-regulated genes and 258 down-regulated genes. Among these, NDRG2 may play an important role in the milk fat synthesis in BMECs. Overexpression of NDRG2 significantly enhanced milk fat synthesis and the expression of genes involved in fatty acid synthesis, activation, and transcriptional regulation in BMECs. Conversely, NDRG2 inhibition notably reduced milk fat synthesis in these cells. Further mechanistic investigations demonstrated that NDRG2 overexpression suppressed the AMPK/SREBP1 axis, thereby facilitating milk fat synthesis in BMECs. Additionally, NDRG2 was found to interact with ACC1, and its overexpression promoted milk fat synthesis through this interaction. These findings establish NDRG2 as a potential target for enhancing milk fat production in dairy cows.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"321 ","pages":"Article 146448"},"PeriodicalIF":8.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0