International Journal of Biological Macromolecules最新文献

{"title":"Cellulose/chitosan film based triboelectric-piezoelectric coupled nanogenerator for wearable mechanosensing and energy harvesting","authors":"Zewei Ye,&nbsp;Qingyu Meng,&nbsp;Haomeng Yu,&nbsp;Shitao Shi,&nbsp;Yuanyuan Wang,&nbsp;Zhuyue Lan,&nbsp;Jiaqi Liao,&nbsp;Qingfeng Sun,&nbsp;Xiaoping Shen","doi":"10.1016/j.ijbiomac.2025.144647","DOIUrl":"10.1016/j.ijbiomac.2025.144647","url":null,"abstract":"<div><div>Intelligently harnessing energy from the surrounding environment through various nanogenerators presents an ideal avenue to enable self-powered electronics. Herein, we developed a lightweight, flexible bilayer-structure coupled nanogenerator composed of a Hydroxypropyl cellulose (HPC)/chitosan (CTS)/carbon nanotube (CNT) film as the positive triboelectric layer, (PVDF)/polydimethylsiloxane (PDMS) aerogel as the negative layer. The triboelectric positive layer incorporates HPC and CTS biopolymers with multiple functional groups, thereby enhancing the polarity of the positive layer. Meanwhile, the incorporation of CNT in the biopolymer triboelectric film diminishes the contact impedance of the film, further increasing the voltage output (V_OC). The negative PVDF/PDMS aerogel layer, which exhibits both triboelectric and piezoelectric functions, features a high specific surface area, thus allowing for the coexistence of triboelectric and piezoelectric charges. This triboelectric-piezoelectric coupled nanogenerator displays an impressive sensitivity of 112.5 mV kPa⁻¹, excellent stability (maintaining consistent V_OC over 10,000 cycles), mechanical robustness, and robust energy harvesting capabilities. The simultaneous generation of triboelectric charges and piezoelectric charges due to the high specific surface area is proposed for the first time, offering significant promise for diverse applications ranging from various mechano-sensing to mechanical energy collection and storage.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"315 ","pages":"Article 144647"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poria cocos polysaccharides ameliorate AOM/DSS-induced colorectal cancer in mice by remodeling intestinal microbiota composition and enhancing intestinal barrier function","authors":"Lin Chen ,&nbsp;Shijie Zhao ,&nbsp;Qianyu Chen ,&nbsp;Piao Luo ,&nbsp;Xuhong Li ,&nbsp;Yuxuan Song ,&nbsp;Shenghai Pan ,&nbsp;Qingmei Wu ,&nbsp;Yue Zhang ,&nbsp;Xiangchun Shen ,&nbsp;Yan Chen","doi":"10.1016/j.ijbiomac.2025.144477","DOIUrl":"10.1016/j.ijbiomac.2025.144477","url":null,"abstract":"<div><div>Colorectal cancer (CRC) remains a leading cause of cancer-related mortality, necessitating the development of novel therapeutic strategies. <em>Poria cocos</em> polysaccharides (PCP), bioactive components of the traditional medicinal fungus <em>Poria cocos</em>, exhibit significant anticancer potential. This study investigates the protective effects of PCP against azoxymethane/dextran sulfate sodium (AOM/DSS)-induced CRC in mice, with a focus on its impact on intestinal microbiota composition, intestinal barrier integrity, and inflammatory responses. PCP treatment significantly reduced tumor incidence, tumor size, and tumor burden while improving histopathological features and inhibiting Ki67-positive cell proliferation. Mechanistically, PCP enhanced intestinal barrier function by restoring tight junction proteins (E-cadherin, ZO-1, Claudin-3) and mucin secretion (MUC2), thereby reducing intestinal permeability and systemic lipopolysaccharide (LPS) levels. Furthermore, PCP exhibited potent anti-inflammatory effects by downregulating pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and upregulating the anti-inflammatory cytokine IL-10. 16S rRNA sequencing results revealed that PCP modulated the intestinal microbiota, decreasing pathogenic bacteria such as Helicobacter and Eisenbergiella while promoting beneficial taxa including Limosilactobacillus, Paraprevotella, and Muribaculum. Fecal microbiota transplantation (FMT) further confirmed the microbiota-mediated protective effects of PCP, as FMT from PCP-treated donors significantly suppressed tumorigenesis, restored intestinal barrier integrity, and alleviated inflammation in CRC mice. Additionally, PCP demonstrated a favorable safety profile, with no adverse effects on major organs. These findings highlight PCP as promising natural agents for CRC prevention and therapy, acting through modulation of the intestinal microbiota, enhancement of intestinal barrier function, and suppression of inflammation.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"315 ","pages":"Article 144477"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hybrid protocol for peptide development: integrating deep generative models and physics simulations for biomolecular design targeting IL23R/IL23","authors":"Naila Qayyum ,&nbsp;Hana Seo ,&nbsp;Noman Khan ,&nbsp;Abdul Manan ,&nbsp;Rajath Ramachandran ,&nbsp;Muhammad Haseeb ,&nbsp;Eunha Kim ,&nbsp;Sangdun Choi","doi":"10.1016/j.ijbiomac.2025.144652","DOIUrl":"10.1016/j.ijbiomac.2025.144652","url":null,"abstract":"<div><div>Recent advances in machine learning have revolutionized molecular design; however, a gap remains in integrating generative models with physics-based simulations to develop functional modulators, such as stable peptides, for challenging targets like the interleukin-23 receptor (IL23R) and its associated cytokine, interleukin-23 (IL23). The IL23R/IL23 axis plays a critical role in autoimmune diseases, and current therapies have largely been limited to antibody-based approaches. To address this gap, we employed a hybrid computational approach that combines Long Short-Term Memory (LSTM) networks for peptide generation, a Gated Recurrent Unit (GRU)-based classifier for anti-inflammatory property prediction, and molecular dynamics (MD) simulations to assess structural dynamics, binding interactions, as well as key properties such as binding affinity and stability. Using this hybrid framework, we identified novel inhibitory peptides, particularly P4, with an IC₅₀ of 2 μM. Systematic experimental validation established its inhibitory activity, elucidated its binding mechanism, confirmed its specificity toward the IL23R, and demonstrated its ability to disrupt IL23R/IL23 interaction. This integrated approach highlights the significant potential of combining deep learning and simulations to accelerate the identification of peptide-based therapeutics targeting key protein targets.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144652"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing activity and stability of a GH8 chitosanase through conserved N-terminal and peripheral residue mutations for bioactive chitooligosaccharide production","authors":"Lige Tong ,&nbsp;Yuanying Li ,&nbsp;Qijian Qin ,&nbsp;Yi Yu ,&nbsp;Lian Duan ,&nbsp;Xiao Wang ,&nbsp;Yongqiang Jiang ,&nbsp;Guiyan Liao ,&nbsp;Yiwen Zhang ,&nbsp;Chen Wu ,&nbsp;Bin Wang ,&nbsp;Wenxia Fang","doi":"10.1016/j.ijbiomac.2025.144643","DOIUrl":"10.1016/j.ijbiomac.2025.144643","url":null,"abstract":"<div><div>Chitosan, the second most abundant biopolymer after cellulose, has vast application potential. However, its high viscosity and poor solubility have limited its full utilization. Chitosan degradation primarily depends on GH46 family chitosanases, while the more stable GH8 family chitosanases have received less attention. This study, for the first time, reveals the critical role of the conserved N-terminal region in the catalytic activity and substrate binding of the GH8 family chitosanase <em>Bc</em>Cn8A using truncation mutagenesis and molecular dynamics simulations. Through evolutionary analysis and computationally assisted semi-rational design, the optimized mutant <em>Bc</em>Cn8A-ΔN4-V319L was obtained, showing a 310 % increase in specific activity and a 5 °C increase in optimal temperature. The engineered enzyme efficiently hydrolyzes chitosan into chitooligosaccharides (COSs) with antioxidant and anti-browning properties, making it suitable for applications like juice preservation. This work provides new insights into the regulatory roles of the N-terminal region and distal amino acid residues in GH8 family chitosanases, advancing their potential in functional oligosaccharide production.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"315 ","pages":"Article 144643"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144137791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disassembly and reassembly of AP205 virus-like particles and the removal of bound RNA for cargo encapsulation","authors":"Zhi Wei Wong,&nbsp;Daiwen Yang","doi":"10.1016/j.ijbiomac.2025.144641","DOIUrl":"10.1016/j.ijbiomac.2025.144641","url":null,"abstract":"<div><div><em>Acinetobacter</em> phage 205 (AP205) is a single-stranded RNA virus. The AP205 capsid protein (CP) spontaneously self-assembles to form virus-like particles (VLPs). VLPs have been widely used in vaccine development due to their high immunogenicity and show great potential for drug encapsulation and delivery. However, recombinant AP205 VLPs contain significant quantities of host cell RNA, which hinders their implementation as therapeutics. Furthermore, the relationship between AP205 CP structure and its roles in VLP stability and RNA association remains poorly understood. Here, we developed a method – predominantly mediated by the chaotropic effect of urea – to disassemble and reassemble AP205 VLPs with high fidelity (&gt;90 %). We also identified the AP205 RNA binding site at Lys14 and Ser30 and generated an AP205 mutant that successfully abrogated RNA binding while retained the ability to self-assemble into RNA-free VLPs. Building on these findings, we assessed the encapsulation of biomolecules such as RNA and proteins within AP205 VLPs and demonstrated that proteins as large as 38 kDa could be encapsulated within our RNA-free AP205 VLPs. Our findings present a homogenous, RNA-free VLP construct suitable for vaccine development and introduce a novel approach for the delivery of biomolecules and drugs through AP205 VLP cargo encapsulation.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"315 ","pages":"Article 144641"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Protein-based nanocarriers for paclitaxel (PTX) delivery in cancer treatment: A review\" [Int. J, Biol. Macromol. 310 (2025), 143068].","authors":"Sakineh Abbaspour, Mohamadsadegh Mohamadzadeh, Seyed Abbas Shojaosadati","doi":"10.1016/j.ijbiomac.2025.144555","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.144555","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"144555"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced biomechanical compatibility of 3D-printed polylactic acid lattice structures: Synergizing mechanical, topography, and microstructural properties for trabecular bone mimicry.","authors":"Ray Tahir Mushtaq, Mudassar Rehman, Chengwei Bao, Yanen Wang, Aqib Mashood Khan, Shubham Sharma, Saqib Anwar","doi":"10.1016/j.ijbiomac.2025.144373","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.144373","url":null,"abstract":"<p><p>The design and mechanical performance of 3D-printed lattice scaffolds are critical for biomedical applications, particularly when replicating the trabecular architecture of bone. This study evaluated the mechanical and biological performance of collagen-infused PLA 3D-printed lattice scaffolds designed for trabecular bone regeneration. Four geometries-body-centered cubic (BCC), diamond, gyroid, and rhombic-were fabricated with cellular wall thicknesses of 1.5, 2.0, and 2.5 mm. BCC lattices achieved a maximum compressive strength of 14.66 MPa, while Diamond-2 samples recorded a yield strength of 2.61 MPa. Gyroid scaffolds, though not the strongest, exhibited optimal porosity (up to 9.98 %) and the highest surface roughness (Sa = 12.51 μm), features that enhance cell attachment. In vitro assays with L929 fibroblast cells revealed that transparent PLA analogues of the gyroid design achieved relative growth rates of 109.4 % and 125.7 % at 50 % and 100 % extraction concentrations, respectively, compared to 37.3 % and 31.1 % for green PLA analogues at 60 % and 100 % extraction concentrations. These results underscore that while BCC structures excel in mechanical support, gyroid lattices provide a superior balance between mechanical integrity and biological performance, rendering them promising candidates for bone tissue engineering. These findings offer important insights for optimizing collagen-enhanced, 3D-printed scaffolds tailored to meet the dual mechanical and biological demands of trabecular bone regeneration.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"144373"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel approach: Lignin extraction from sequentially pretreated sugarcane trashes to produce prospective nanolignin/PVA biosurfactants","authors":"Azizatul Karimah ,&nbsp;Nyoman Jaya Wistara ,&nbsp;Widya Fatriasari ,&nbsp;M. Hazwan Hussin","doi":"10.1016/j.ijbiomac.2025.144425","DOIUrl":"10.1016/j.ijbiomac.2025.144425","url":null,"abstract":"<div><div>A sustainable and eco-friendly approach for utilizing sugarcane trash (SCT) biomass is currently under development. The SCT was subjected to a sequential pretreatment process involving microwave irradiation and autoclave heating before extracting alkali lignin. Nanolignin was then synthesized from the extracted lignin. The particle size of the synthesized nanolignin ranged between 65 and 84 nm, according to morphological characterization. This nanolignin was then transformed into a nanolignin/polyvinyl alcohol (PVA) biosurfactant via an eco-friendly solvent. The surface tension and functional groups of the biosurfactant were evaluated. The yield of lignin obtained from SCT varied between 25 % and 28 %, with the same purity of 70 %. The physical, chemical, and morphological characteristics of isolated lignin are comparable, making it possible to produce nanolignin for biosurfactant synthesis. The presence of aryl–O stretching (1200 cm⁻¹) and 1,4–disubstituted C<img>H bending (800 cm⁻¹) peaks of absorbance in the infrared spectra indicated that the grafting of nanolignin into PVA occurred in the phenolic group of lignin. The biosurfactant nanolignin from autoclave treatment has a low surface tension of 42.20 mN/m, making it a more environmentally friendly additive in lignocellulose hydrolysis.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144425"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microencapsulation of Angelica sinensis essential oil by complex coacervation using chitosan and gelatin: Optimization, characterization, in vitro digestion, and biological activity","authors":"Litao He ,&nbsp;Qinmei Xu ,&nbsp;Wenjuan Xin ,&nbsp;Hong Gu ,&nbsp;Yang Lin ,&nbsp;Peilong Sun","doi":"10.1016/j.ijbiomac.2025.144365","DOIUrl":"10.1016/j.ijbiomac.2025.144365","url":null,"abstract":"<div><div><em>Angelica sinensis</em> essential oil (AEO), a valuable flavoring component and potential anticancer agent, possesses a distinctive odor and strong bioactivity. In this study, AEO was encapsulated using complex coacervation of gelatin (Ge) and chitosan (Chi), with the hypothesis that complex coacervation could be used to develop microcapsules capable of protecting AEO from oxidative reactions while regulating volatile compounds released during digestion. The higher encapsulation efficiency (82.68 ± 0.38 %) was achieved, as optimized by Box-Behnken design and a response surface methodology, using a wall-core ratio of 1.50:1, Ge:Chi mixing ratio of 10.38:1, and pH of 5.33. Microencapsulation of AEO permitted rapid initial release of phthalides and monoterpenes, while facilitating progressive liberation of sesquiterpenes with lower volatility. A rapid release of AEO from microcapsules occurred within the initial 120 min under pH conditions simulating gastrointestinal environments.. Furthermore, <em>Angelica sinensis</em> essential oil microcapsules (AEOM) exhibit strong antioxidant activity and bacteriostatic properties, making them effective in protecting essential oil components and enhancing their potential as functional food ingredients.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144365"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon nanotube-wrapped particles in sodium alginate hydrogels for enhanced solar evaporation and EMI shielding through tortuous channel","authors":"Jing Chen ,&nbsp;Haorong Li ,&nbsp;Baoshu Chen ,&nbsp;Xuena Li ,&nbsp;Dahu Yao ,&nbsp;Xiping Gao ,&nbsp;Chang Lu ,&nbsp;Xinchang Pang","doi":"10.1016/j.ijbiomac.2025.144501","DOIUrl":"10.1016/j.ijbiomac.2025.144501","url":null,"abstract":"<div><div>The hydrogel evaporator has shown great potential in solar-driven seawater desalination. However, hydrogels often suffer from heat loss due to their high-water content. Therefore, achieving a balance between energy input and water supply in hydrogels remains a challenge. Herein, Carbon Nanotube-Wrapped Polyacrylamide (CP) particles were introduced into sodium alginate (SA) to prepare CPSA hydrogels with tortuous channels. The hydrogel achieves a balance between photothermal conversion and water supply by enhancing its photothermal performance and increasing the water transport distance. Under 1.0 sun irradiation, the CPSA hydrogel exhibits an evaporation rate of 1.97 kg m⁻² h⁻¹. Additionally, the CPSA hydrogel demonstrates excellent photocatalytic ability for wastewater treatment, such as methylene blue (MB) degradation. Moreover, the tortuous microstructure imparts the CPSA hydrogel (thickness: 6 mm) with an outstanding electromagnetic interference shielding effectiveness of 81.1 dB, primarily through absorption. This work provides new insights into the development of hydrogels for water-energy balance management and high electromagnetic wave shielding applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"315 ","pages":"Article 144501"},"PeriodicalIF":7.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}