International Journal of Biological Macromolecules最新文献_第10页

Consistency of gene expression and protein acetylation modification with physiological and biochemical changes responding to the deficiency of histone deacetylase 1 homolog in Monascus ruber

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 DOI: 10.1016/j.ijbiomac.2025.142485

Fufang Tang , Yuehan Zhao , Zejing Mao , Yueyan Huang , Yifan Hu , Baixue Liu , Yanchun Huang , Yanchun Shao

{"title":"Consistency of gene expression and protein acetylation modification with physiological and biochemical changes responding to the deficiency of histone deacetylase 1 homolog in Monascus ruber","authors":"Fufang Tang , Yuehan Zhao , Zejing Mao , Yueyan Huang , Yifan Hu , Baixue Liu , Yanchun Huang , Yanchun Shao","doi":"10.1016/j.ijbiomac.2025.142485","DOIUrl":"10.1016/j.ijbiomac.2025.142485","url":null,"abstract":"<div><div>Monascus species can produce several secondary metabolites (SMs), which are regulated by a complex network. Histone deacetylase 1 (Hda1) has been demonstrated as a critical regulator of SMs and developmental processes; however, little is known about Hda1 in Monascus spp. (named MrHda1). In this study, strain ΔMrHda1 was generated for phenotypic assays. The results indicated that MrHda1 inactivation led to reduced conidia and ascospores but significantly increased pigment and citrinin production. Transcriptome data showed that MrHda1 deletion upregulated the expression of most genes in the glycolytic pathway as well as the gene clusters involved in pigment and citrinin biosynthesis. Western blotting (WB) and label-free acetylome data indicated that MrHda1 deletion significantly enhanced lysine acetylation modifications on the H3 subunit and enzymes involved in the citric acid cycle. Site-directed mutation demonstrated that the production of pigments and citrinin directly correlates with the acetylation level of histone H3 lysine 18 (H3K18). These findings provide new insights into the essential functions of MrHda1 and its regulation mechanisms on the SMs.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142485"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breathable and imperceptible on-skin electronic tattoos with a hybrid of silk and cellulose and highly conductive electrodes for monitoring skin hydration

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 DOI: 10.1016/j.ijbiomac.2025.142707

Juwan Choi , Soohoon Lee , Shalik Ram Joshi , Sunghwan Kim

{"title":"Breathable and imperceptible on-skin electronic tattoos with a hybrid of silk and cellulose and highly conductive electrodes for monitoring skin hydration","authors":"Juwan Choi , Soohoon Lee , Shalik Ram Joshi , Sunghwan Kim","doi":"10.1016/j.ijbiomac.2025.142707","DOIUrl":"10.1016/j.ijbiomac.2025.142707","url":null,"abstract":"<div><div>Skin, the largest organ protecting the body, acts as a pathway connecting the inside and outside of the body, allowing us to examine health conditions. Therefore, on-skin electronics are attractive for monitoring biosignals in daily life for point-on-care healthcare. However, integrating highly conductive electrodes while maintaining all the properties suitable for on-skin devices, such as flexibility, imperceptibility, breathability, and biocompatibility, is still challenging. Here, we present breathable and imperceptible electronic tattoos (E-tattoos), on which highly conductive gold (Au) electrodes are integrated. The E-tattoo, which a hybrid of two nanostructured biomaterials, ultrathin silk film and cellulose nanofiber mat, possesses all favorable properties for on-skin electronics. Due to the inherent strong adhesion of silk to Au, patterned Au electrodes, with a high conductivity (2.84 × 107 S/m) comparable to that of pure Au (4.01 × 107 S/m), can be integrated on the E-tattoo. High water-vapor transmission and low leakage current through E-tattoos provide skin-compatibility (nonirritating response). With these advantages, the E-tattoo is applied to monitor skin hydration. On-skin impedance measurements reveal dependency on skin hydration, and impedances measured with E-tattoos show better signal stability than those measured for Au nanomesh patches. This study presents a new on-skin electronic platform for monitoring skin conditions.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142707"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical synthesis of biological grafted starch with poly(N, N-dimethyl acrylamide) and poly(ethyl acrylate) structure for conferring superior paste stability, adhesion to cellulose-based cotton, film properties, and desizability

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 DOI: 10.1016/j.ijbiomac.2025.142719

Wei Li , Qian Liu , Xun Zhang , Yingfeng Wang , Maoli Yin , Xuchen Tao

{"title":"Chemical synthesis of biological grafted starch with poly(N, N-dimethyl acrylamide) and poly(ethyl acrylate) structure for conferring superior paste stability, adhesion to cellulose-based cotton, film properties, and desizability","authors":"Wei Li , Qian Liu , Xun Zhang , Yingfeng Wang , Maoli Yin , Xuchen Tao","doi":"10.1016/j.ijbiomac.2025.142719","DOIUrl":"10.1016/j.ijbiomac.2025.142719","url":null,"abstract":"<div><div>This study mainly aimed to reveal the superiority of the positively charged poly(N, N-dimethyl acrylamide) (PC-PDMAA)/poly(ethyl acrylate) (PEA) structure to control poly(sodium allyl sulfonate) (PSAS)/PEA structure in enhancing the sizing properties (viscosity stability, adhesion to fibers (cellulose-based cotton and hydrophobic polyester), and film properties) and desizability of starch, and avoid desizing difficulty issue of positively charged PATAC-grafted starch, due to its worse desizing efficiency. Also, it aimed to survey the influence of grafting ratio on these properties, to develop a new biobased starch adhesive, PC-PDMAA-g-starch-g-PEA (PC-PDMAA-g-S-g-PEA), for the sizing of cotton and polyester. Under a similar grafting ratio, the PC-PDMAA-g-S-g-PEA (IV) showed significantly superior bonding to both fibers, film elongation and endurance (p < 0.05), and better paste stability and desizability compared to control PSAS-g-starch-g-PEA (PSAS-g-S-g-PEA, IV#). This demonstrated that combining the PC-PDMAA/PEA branches could further enhance these properties of acid-thinned starch (ATS) compared to the PSAS/PEA branches. Bonding strengths, viscosity stability, desizability, film elongation, and film endurance of PC-PDMAA-g-S-g-PEA were strengthened with an increased grafting ratio from 0 % to 10.80 %. According to these findings, PC-PDMAA-g-S-g-PEA, a novel starch bio-based adhesive with a grafting ratio of 10.80 %, showed promise for use in cotton and polyester warp sizing.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142719"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lignin-based biochar with improved properties derived from the microbial combined chemical pretreatment of corn straw for efficient antibiotic removal

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 DOI: 10.1016/j.ijbiomac.2025.142739

Yiping Jin, Zhongwen Zhou, Zekai Yuan, Runjia Hu, Tianran Zhang, Siji Chen, Guang Chen

{"title":"Lignin-based biochar with improved properties derived from the microbial combined chemical pretreatment of corn straw for efficient antibiotic removal","authors":"Yiping Jin, Zhongwen Zhou, Zekai Yuan, Runjia Hu, Tianran Zhang, Siji Chen, Guang Chen","doi":"10.1016/j.ijbiomac.2025.142739","DOIUrl":"10.1016/j.ijbiomac.2025.142739","url":null,"abstract":"<div><div>In this study, straw lignin was separated from corn straw by combining microbial treatment with ρ-TsOH organic acid extraction for the first time, and lignin-derived biochars were prepared by carbonation-activation. The results showed that the extraction rate of lignin and the physicochemical properties of related biochars were improved greatly by microbial treatment. The specific surface areas of lignin biochar obtained after combined pre-treatment with Aspergillus niger, Myrothecium verrucaria, and Trichoderma reesei, (BNL, BML, and BTL) were 2348, 2849 and 3008 m2 g−1, respectively, and the total pore volumes were 0.8989, 0.9411, and 1.2621 cm3 g−1, which were significantly higher than the 2292 m2 g−1 and 0.7786 cm3 g−1 of the control group BCL (biochar prepared from lignin extracted from raw straw). In adsorption experiments by using tetracycline hydrochloride and sodium sulfadiazine as antibiotic models, the maximum adsorption capacities of all lignin-derived biochars (BML, BTL, and BNL) were greater than that of most other adsorbents including BCL. We hope this work could provide a new strategy for efficiently using microbial treatment technology to improve the conversion of lignocellulosic resources.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142739"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erucic acid promotes intramuscular fat deposition through the PPARγ-FABP4/CD36 pathway. 芥酸通过PPARγ-FABP4/CD36途径促进肌内脂肪沉积。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140121

Hengwei Yu, Juntao Guo, Bingzhi Li, Jing Ma, Belete Kuraz Abebe, Chugang Mei, Sayed Haidar Abbas Raza, Gong Cheng, Linsen Zan

{"title":"Erucic acid promotes intramuscular fat deposition through the PPARγ-FABP4/CD36 pathway.","authors":"Hengwei Yu, Juntao Guo, Bingzhi Li, Jing Ma, Belete Kuraz Abebe, Chugang Mei, Sayed Haidar Abbas Raza, Gong Cheng, Linsen Zan","doi":"10.1016/j.ijbiomac.2025.140121","DOIUrl":"10.1016/j.ijbiomac.2025.140121","url":null,"abstract":"The regulation of intramuscular fat (IMF) accumulation plays a crucial role in determining meat quality in the beef industry. In humans, fat deposition in skeletal muscle is closely associated with insulin resistance and obesity. However, its underlying mechanisms are not fully elucidated. We previously identified erucic acid (EA) as a key metabolite that may affect IMF deposition of beef using omics strategies. By utilizing bovine intramuscular preadipocytes in vitro, the study demonstrates a dose-dependent increase in lipid storage induced by EA, along with mRNA expression levels of transporters FABP4 and CD36. At a mechanistic level, EA triggers ERK1/2 phosphorylation and enhances the expression of PPARγ, FABP4, and CD36, thereby facilitating the formation of lipid droplets within preadipocytes. In vivo experiments conducted in mice support these findings, indicating that EA stimulates fat accumulation in skeletal muscles and enhances the levels of FABP4 and CD36 proteins. These outcomes not only enhance our comprehension of the molecular mechanisms governing IMF deposition but also provide insights into potential strategies for enhancing meat quality and addressing metabolic disorders linked to fat accumulation in skeletal muscles. The findings of the study contribute to existing scholarly knowledge and lay the groundwork for future research endeavors aimed at improving meat quality and metabolic well-being.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140121"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "A C-type lectin-like receptor CD302 in yellow drum (Nibea albiflora) functioning in antibacterial activity and innate immune signaling" [Int. J. Biol. Macromol. 247 (2023) 125734].

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-21 DOI: 10.1016/j.ijbiomac.2025.139807

Jia Peng, Jiacheng Li, Jingjie Liang, Wanbo Li, Yao Yang, Yukai Yang, Sen Zhang, Xiaolin Huang, Fang Han

引用次数: 0

Hyaluronic acid-conjugated lipid nanocarriers in advancing cancer therapy: A review.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-20 DOI: 10.1016/j.ijbiomac.2025.140146

Guifeng Zhang, Xin Jiang, Yitong Xia, Pengpeng Qi, Jie Li, Lizhen Wang, Zheng Wang, Xiuli Tian

{"title":"Hyaluronic acid-conjugated lipid nanocarriers in advancing cancer therapy: A review.","authors":"Guifeng Zhang, Xin Jiang, Yitong Xia, Pengpeng Qi, Jie Li, Lizhen Wang, Zheng Wang, Xiuli Tian","doi":"10.1016/j.ijbiomac.2025.140146","DOIUrl":"10.1016/j.ijbiomac.2025.140146","url":null,"abstract":"Lipid nanoparticles are obtaining significant attention in cancer treatment because of their efficacy at delivering drugs and reducing side effects. These things are like a flexible platform for getting anticancer drugs to the tumor site, especially upon HA modification, a polymer that is known to target tumors overexpressing CD44. HA is promising in cancer therapy because it taregtes tumor cells by binding onto CD44 receptors, which are often upregulated in cancer cells. Lipid nanoparticles are not only beneficial in improving solubility and stability of drugs; they also use the EPR effect, meaning they accumulate more in tumor tissue than in healthy tissue. Adding HA to these nanoparticles expands their biocompatibility and makes them more accurate and specific towards tumor cells. Studies show that HA-modified nanoparticles carrying drugs such as paclitaxel or doxorubicin improve how well cells absorb the drugs, reduce drug resistance, and make tumor shrinking. These nanoparticles can respond to tumor microenvironment stimuli in targeted delivery. This targeted delivery diminishes side effects and improves anti-cancer activity of drugs. Thus, lipid-based nanoparticles conjugated with HA are a promising way to treat cancer by delivering drugs effectively, minimizing side effects, and giving us better therapeutic results.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140146"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring biomass derived microcrystalline cellulose from the waste aquatic plant Pistia stratiotes: A comprehensive characterization for polymer composite reinforcement.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-21 DOI: 10.1016/j.ijbiomac.2025.140217

Ajith J Kings, Indran Suyambulingam, Sunesh Narayanaperumal, L R Monisha Miriam, Divya Divakaran, Adhigan Murali, Sung Soo Han

{"title":"Exploring biomass derived microcrystalline cellulose from the waste aquatic plant Pistia stratiotes: A comprehensive characterization for polymer composite reinforcement.","authors":"Ajith J Kings, Indran Suyambulingam, Sunesh Narayanaperumal, L R Monisha Miriam, Divya Divakaran, Adhigan Murali, Sung Soo Han","doi":"10.1016/j.ijbiomac.2025.140217","DOIUrl":"10.1016/j.ijbiomac.2025.140217","url":null,"abstract":"The creation of polymer composites with better performance is a crucial thing. The cellulosic filler material gain popularity in polymer composites. In this study, aquatic plant Pistia stratiote leaves were used as a raw material for cellulose extraction. The cellulose was extracted via acid hydrolysis method with mild concentration chemicals. The main aim was to assess the cellulose characteristics and its potential as a reinforcement for composites. Surface, thermal, and physicochemical properties of the micro fillers made of cellulose were the primary areas of research. To determine the composition of the cellulose, extensive chemical characterization analyses were conducted. According to X-ray diffraction studies, Pistia stratiotes leaves cellulose have a crystallinity index of 75.9 % and crystalline size of 8.2 nm. Cellulosic functional groups were revealed by examination using a Fourier Transform Infrared Spectrometer. Scanning electron microscopy images revealed smooth surface and distorted shaped particles. The average particle size, which was calculated using the ImageJ software, was 23.253 ± 6.55 μm. The extracted micro cellulose had an acceptable average roughness value of 28.296 μm, as shown by atomic force microscopy images. Surface properties of the Pistia stratiotes leaves cellulose (PSC) were shown to be conducive to the formation of interfacial bonds with other matrices while composites are being built. The BET surface areas are significantly higher as well. The material degrades only at high temperatures 215 °C, which was analysed by TG analysis. The findings demonstrate that Pistia stratiotes, a plant, outperforms more conventional sources of micro cellulose, such as cotton, hemp, and wood. As a greener alternative to synthetic reinforcements, the recovered micro cellulose has potential uses across numerous industries.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140217"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized peptide inhibitor Aqs1C targets LasR to disrupt quorum sensing and biofilm formation in Pseudomonas aeruginosa: Insights from MD simulations and in vitro studies.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-22 DOI: 10.1016/j.ijbiomac.2025.140119

Hani A Alhadrami, Ahmed M Sayed, Hossam M Hassan, Mostafa E Rateb, Mostafa N Taha

{"title":"Optimized peptide inhibitor Aqs1C targets LasR to disrupt quorum sensing and biofilm formation in Pseudomonas aeruginosa: Insights from MD simulations and in vitro studies.","authors":"Hani A Alhadrami, Ahmed M Sayed, Hossam M Hassan, Mostafa E Rateb, Mostafa N Taha","doi":"10.1016/j.ijbiomac.2025.140119","DOIUrl":"10.1016/j.ijbiomac.2025.140119","url":null,"abstract":"Pseudomonas aeruginosa (PA) is a critical pathogen, and its antibiotic resistance is largely driven by the quorum-sensing regulator LasR. Herein, we report the design, synthesis, and characterization of Aqs1C, a mutated peptide derivative of Aqs1, optimized to inhibit LasR and its quorum-sensing pathway. By introducing a targeted mutation, Aqs1C exhibited enhanced stability and binding affinity for LasR protein compared to its predecessor, Aqs1B. Using molecular dynamics simulations (MDS), the Aqs1C-LasR complex demonstrated a marked increase in structural stability, reflected in reduced root mean square deviation (RMSD) values and lower binding free energy. Electrostatic complementarity analysis showed stronger and more favorable interactions between Aqs1C and LasR. Further, GaMD experiments were able to reproduce the binding state between Aqs1C and LasR, indicating the binding mechanism between them. These molecular insights correlated with functional in vitro assays. Aqs1C effectively inhibited quorum-sensing-associated virulence factors in PA, involving biofilm formation (77.6 % inhibition), pyocyanin production (75.7 % inhibition), protease secretion (61.1 % inhibition), and rhamnolipid production (74.1 % inhibition), at a 100 μg/mL concentration, in a comparable or superior pattern to azithromycin (AZM). Molecular modelling, MDS, and GaMD insights and in vitro assays established Aqs1C as a promising candidate for therapeutic development to mitigate PA infections through targeted quorum-sensing disruption.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140119"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An acid polysaccharide from Mentha haplocalyx exerts the antifatigue effect via activating AMPK.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-24 DOI: 10.1016/j.ijbiomac.2025.140235

Xiang Ji, Shenghua Chen, Qifang Wu, Menglai Ling, Jingyang Tong, Haibin Tong, Guanhua Wang, Jiancheng Gong

{"title":"An acid polysaccharide from Mentha haplocalyx exerts the antifatigue effect via activating AMPK.","authors":"Xiang Ji, Shenghua Chen, Qifang Wu, Menglai Ling, Jingyang Tong, Haibin Tong, Guanhua Wang, Jiancheng Gong","doi":"10.1016/j.ijbiomac.2025.140235","DOIUrl":"10.1016/j.ijbiomac.2025.140235","url":null,"abstract":"Fatigue is a pathological state that can impair physical and cognitive performance, making the development of effective therapeutic strategies crucial. In this study, an acid polysaccharide (MHa) was isolated from Mentha haplocalyx. Structural analysis showed that MHa (40.7 kDa) has a backbone consisting of 4-α-GalAp, 6-α-Galp, and 4,6-α-Galp, with branches at the C6 of 4,6-α-Galp linked to four distinct side chains, including 4-α-Galp, 3,6-β-Manp, t-α-Araf, t-α-Rhap, t-α-Glcp, and t-β-Rhap. MHa possesses a triple-helix conformation with a sheet-like appearance, which may contribute to its biological stability and activity. Functionally, MHa exhibited significant antifatigue effects, with the 400 mg/kg dose showing the most potent activity. Compared to the model group, treatment with 400 mg/kg of MHa increased the exhaustive swimming time by 1.89-fold in fatigued mice, reduced blood lactate and urea nitrogen levels by 24.21 % and 35.57 %, respectively, and enhanced liver glycogen, muscle glycogen, and ATP levels by 20.08 %, 46.52 %, and 50.43 %, respectively. MHa improved the activities of Ca2+-Mg2+-ATPase and Na+-K+-ATPase, while also enhancing antioxidant defense. Mechanistically, MHa promotes mitochondrial biogenesis and enhances oxidative defense via activating AMPK. These findings highlight the potential of MHa as a promising candidate for developing antifatigue supplements, offering a novel strategy to mitigate fatigue.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140235"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0