International Journal of Biological Macromolecules最新文献_第10页

Hairy chitin nanocrystals: Sustainable adsorbents for efficient removal of organic dyes.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-17 DOI: 10.1016/j.ijbiomac.2025.139948

Qian Ding, Chunyu Ji, Ting Wang, Yifeng Wang, Han Yang

{"title":"Hairy chitin nanocrystals: Sustainable adsorbents for efficient removal of organic dyes.","authors":"Qian Ding, Chunyu Ji, Ting Wang, Yifeng Wang, Han Yang","doi":"10.1016/j.ijbiomac.2025.139948","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139948","url":null,"abstract":"Chitin nanocrystals (ChNCs) are typically produced using a combination of strong acid hydrolysis and mechanical treatments. In this study, a mild, energy-efficient, and environmentally friendly method was developed to prepare a novel form of chitin nanocrystals called hairy chitin nanocrystals (HChNCs) without the need of any mechanical treatments. The HChNCs were made by sequential oxidations on partially deacetylated chitin, resulting in a unique morphology with chitin molecular chains protruding from central chitin nanorods. These HChNCs have a high content of carboxyl groups, reaching up to 2.72 mmol/g, which enhances their ability to adsorb methylene blue (MB) effectively. Within just 1 min, the HChNCs can adsorb as much as 909.11 ± 17.44 mg/g of MB, a significantly higher capacity compared to other chitin-derived materials. Additionally, the HChNCs showed remarkable synergistic removal efficiency for both MB and methyl orange (MO) in a mixed dye system, making them a promising sustainable option for treating wastewater from industrial printing and dyeing processes.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"139948"},"PeriodicalIF":7.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the SP gene family and transcription factor SlSP5G promotes the high-temperature tolerance of tomatoes.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-17 DOI: 10.1016/j.ijbiomac.2025.140043

Wei Li, Shuchao Huang, Xiting Yang, Yandong Xie, Xin Meng, Zhiqi Xu, Zhaozhuang Li, Wenhao Zhou, Wei Zhang, Shuya Wang, Li Jin, Ning Jin, Jian Lyu, Jihua Yu

{"title":"Identification of the SP gene family and transcription factor SlSP5G promotes the high-temperature tolerance of tomatoes.","authors":"Wei Li, Shuchao Huang, Xiting Yang, Yandong Xie, Xin Meng, Zhiqi Xu, Zhaozhuang Li, Wenhao Zhou, Wei Zhang, Shuya Wang, Li Jin, Ning Jin, Jian Lyu, Jihua Yu","doi":"10.1016/j.ijbiomac.2025.140043","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140043","url":null,"abstract":"Some members of the SELF PRUNING (SP) gene family have been shown to play critical roles in developmental processes and stress responses across a wide range of plant species. The study identifies 13 members that can be divided into three subfamilies based on evolutionary analysis. Cis-Acting element analysis of the promoter regions indicated the presence of numerous stress- and hormone-responsive elements in the SlSP family. Subcellular localization analysis showed that the SlSP family proteins are localized in the cell membrane, nucleus, and chloroplasts. Notably, the expression of SELF PRUNING 5G (SlSP5G) was significantly induced by high-temperature stress. Silencing SlSP5G reduced tolerance to high-temperature stress. Conversely, its overexpression in stable transgenic lines enhanced heat tolerance, as demonstrated by improved membrane stability, elevated antioxidant enzyme activity, and reduced reactive oxygen species (ROS) accumulation. In contrast, SlSP5G knockout lines were more susceptible to high-temperature stress. This study provides a comprehensive analysis of the SlSP gene family, offering novel insights into the mechanism of SlSP5G-mediated heat stress tolerance in tomato.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"140043"},"PeriodicalIF":7.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-regulated preparation and structural characterization of non-covalent complexes of soybean isolate proteins with different charged polysaccharides.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-17 DOI: 10.1016/j.ijbiomac.2025.140004

Jiaying Chen, Yilin Wang, Mingxia Pu, Shan He, Neethu Ninan, Ming Cheng

{"title":"pH-regulated preparation and structural characterization of non-covalent complexes of soybean isolate proteins with different charged polysaccharides.","authors":"Jiaying Chen, Yilin Wang, Mingxia Pu, Shan He, Neethu Ninan, Ming Cheng","doi":"10.1016/j.ijbiomac.2025.140004","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140004","url":null,"abstract":"Soybean protein isolate (SPI) exhibits limited functional properties in processing applications due to environmental stressors such as pH, salt ion, and temperature. The present study was devoted to exploring the non-covalent assembly of SPI with chitosan (CS), glucan (GL) and sodium alginate (SA) under different pH conditions. At a fixed mixing ratio (1:1), the phase behavior, protein solubility, and surface hydrophobicity (H0) of the resulting protein-polysaccharide complexes (PPCs) exhibited great differences due to the diversity of polysaccharide charge density and structure. Specifically, CS and SA primarily incorporated with SPI through electrostatic interactions, resulting in a pronounced enhancement of SPI solubility near the isoelectric point, with increases of 37.1 % and 51.6 %, respectively. In contrast, the combination of GL with SPI dominated by hydrophobic interactions and hydrogen bonds, yielding a similar protein solubility and H0 to SPI itself under different pH. Further analysis in charge density indicates that heat treatment promotes the electrostatic complexation of proteins with polysaccharides, whereas an increase in ionic strength inhibits the non-covalent assembly, and this effect was pronounced in the anionic polysaccharide system. In addition, the formation of electrostatic complexes exerted a positive effect on the stability of the emulsions, while the co-soluble systems tended to produce emulsion particles with smaller particle sizes. In summary, the charged polysaccharides showed great potential to modulate protein structure and enhance the stability of protein emulsions compared with the nonionic polysaccharides.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140004"},"PeriodicalIF":7.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NAA enhances armillaria gallica growth by modulating nitrogen metabolism through AgZFP48.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-17 DOI: 10.1016/j.ijbiomac.2025.140032

Xing Zhao, Jinlong Cai, Peng Xu, Zeyi Zhou, Caixia Guo, Hongjun Nian, Kunzhi Li

{"title":"NAA enhances armillaria gallica growth by modulating nitrogen metabolism through AgZFP48.","authors":"Xing Zhao, Jinlong Cai, Peng Xu, Zeyi Zhou, Caixia Guo, Hongjun Nian, Kunzhi Li","doi":"10.1016/j.ijbiomac.2025.140032","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140032","url":null,"abstract":"Armillaria gallica (A. gallica) is a fungus with both medicinal and edible properties. Previous transcriptome analysis has identified the C2H2-type zinc finger transcription factor as a candidate gene involved in the NAA-induced growth promotion of A. gallica. However, the molecular mechanism underlying the enhancement of A. gallica growth by C2H2 transcription factor in response to NAA treatment remains unclear. In this study, we identified a C2H2-type zinc finger transcription factor gene in A. gallica and investigated its function, aiming to elucidate the mechanism by which C2H2-type zinc finger transcription factors regulate the growth of A. gallica. We identified and characterized a novel C2H2-type zinc finger transcription factor, AgZFP48, in A. gallica and found that AgZFP48 is located in the nucleus, where it acts as a transcription activator. AgZFP48 positively regulated the growth of A. gallica. The potential targets of AgZFP48 were identified by using DNA affinity purification sequencing (DAP-seq). In addition, four candidate genes were selected for Electrophoretic Mobility Shift Assays (EMSA) and luciferase reporter activity assessment. The results showed that AgZFP48 activated the expression of ammonium transporter (AgAMT), glutamine synthetase (AgGS), acetylornithine aminotransferase (AgAcOAT), and amino acid permease (AgAAP) by binding to their promoters or exons. In summary, our results suggest that AgZFP48 promotes nitrogen metabolism in A. gallica by activating the expression of nitrogen metabolism-related genes, thereby regulating the growth of the fungus.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140032"},"PeriodicalIF":7.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional customized artificial grafts functionalized with biomimetic softness and anticoagulant heparin-dopamine surface modification: Preclinical study for practical applications.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-17 DOI: 10.1016/j.ijbiomac.2025.140002

Chae Hwa Kim, Yuseok Kim, Sandeep Karna, Sung Mook Yoo, Ju Han Lee, Yun Ju Kim, Jun Hyuk Lee, Won-Min Jo, Suk-Hee Park, Tae Hee Kim

{"title":"Three-dimensional customized artificial grafts functionalized with biomimetic softness and anticoagulant heparin-dopamine surface modification: Preclinical study for practical applications.","authors":"Chae Hwa Kim, Yuseok Kim, Sandeep Karna, Sung Mook Yoo, Ju Han Lee, Yun Ju Kim, Jun Hyuk Lee, Won-Min Jo, Suk-Hee Park, Tae Hee Kim","doi":"10.1016/j.ijbiomac.2025.140002","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140002","url":null,"abstract":"Artificial vascular grafts, as blood vessel substitutes, are a prime challenge in tissue engineering and biomaterial research. An ideal artificial graft must have physiological and mechanical properties similar to those of a natural blood vessel, and hemocompatibility on its surface. We designed and fabricated artificial grafts by applying 3D printing and templated technology, which is endowed with morphologically patient-specific vascular reconstruction. To optimize mechanical properties, the graft wall was engineered with a controllable hybrid porous structure through a multilayer combination of porous and nonporous coatings, thereby achieving biomimetic mechanical flexibility with reduced stiffness. Further, we successfully synthesized Dopamine-conjugated heparin (Hep-DA) utilizing carbodiimide chemistry, and coated it on a 3D porous graft to improve both surface adhesion and anticoagulant ability. The Hep-DA-coated 3D graft did not show significant cytotoxic effects with a long-term sustained heparin release. We performed a preclinical study in swine using the developed graft along with commercially available graft ePTFE and Dacron as a reference. They were implanted in the swine aorta for 28 days and the implanted grafts were harvested for further analysis. Histopathology study results showed the feasibility of the developed artificial vascular grafts that have less calcification, fibrosis, and collagen deposition than commercially available grafts.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140002"},"PeriodicalIF":7.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and physicochemical properties of OSA modified Cyperus esculentus starch nanoparticles.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-17 DOI: 10.1016/j.ijbiomac.2025.140045

Fanhao Meng, Shuangqi Tian, Yanyan Chen, Zehua Liu

{"title":"Preparation and physicochemical properties of OSA modified Cyperus esculentus starch nanoparticles.","authors":"Fanhao Meng, Shuangqi Tian, Yanyan Chen, Zehua Liu","doi":"10.1016/j.ijbiomac.2025.140045","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140045","url":null,"abstract":"The aim of this study was to investigate the Octenyl succinic anhydride (OSA) modification and nanonization of Cyperus esculentus starch (CES) on its physicochemical properties. Cyperus esculentus starch nanoparticles (SNPs) were prepared by nanoprecipitation method and modified with OSA. The results showed that the average particle size of the prepared SNPs was 125.25 nm, and the OSA modified nanoparticles (OS-SNPs) were between 411.16 and 442.07 nm. Chemical group analysis indicated the successful esterification reaction of OSA. Crystallographic structure analysis showed that the crystalline type of CES remained unchanged after OSA modification, but the crystallinity was slightly decreased. Moreover, SNPs exhibited a V-type diffraction peak. Morphological observation results showed that the hydrophobic groups introduced after OSA modification of SNPs had caused particle deformation and formed a network structure. In addition, the gelatinization temperature of starch decreased after OSA modification, and the gelatinization temperature could no longer be measured after nanonization. The OSA modified CES (OS-CES) had shown higher viscosity, swelling power and solubility during the gelatinization process. While the nanonized samples had no obvious viscosity change during the heating process, the solubility remained at a relatively high level of 70-90 %.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140045"},"PeriodicalIF":7.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification, crystal structural characterization of porcine kidney dipeptidyl peptidase IV (PkDPP-IV) and its interaction with oyster derived inhibitory peptide ILAPPER.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-17 DOI: 10.1016/j.ijbiomac.2025.140047

Wan-Yu Li, Hong Chen, Duanquan Lin, Tuo Zhang, Yu-Lei Chen, Tengchuan Jin, Min-Jie Cao

{"title":"Purification, crystal structural characterization of porcine kidney dipeptidyl peptidase IV (PkDPP-IV) and its interaction with oyster derived inhibitory peptide ILAPPER.","authors":"Wan-Yu Li, Hong Chen, Duanquan Lin, Tuo Zhang, Yu-Lei Chen, Tengchuan Jin, Min-Jie Cao","doi":"10.1016/j.ijbiomac.2025.140047","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140047","url":null,"abstract":"Dipeptidyl peptidase IV (DPP-IV) is an important target enzyme for the treatment of type 2 diabetes mellitus (T2DM). Increasing researchers try to screen DPP-IV inhibitory peptides while the cost of DPP-IV is high. In this study, PkDPP-IV was efficiently purified by acid precipitation, ammonium sulfate salting out and gel filtration chromatography with a purification of 283.5 folds and 16.5 % yield. PkDPP-IV is a glycoprotein with molecular weight of 110 kDa and optimal activity at pH 7.0 and 40 °C. Crystal structure indicated that PkDPP-IV is composed of an α/β hydrolase domain and a β-propeller domain, which is highly similar to that of human DPP-IV. A peptide ILAPPER derived from oyster exhibited high inhibitory activity with Ki value of 0.131 μM against PkDPP-IV. The crystal structure of the PkDPP-IV + ILAPPER complex revealed that ILAPPER stably occupy the S1 and S2 catalytic pockets of PkDPP-IV by forming three hydrogen bonds with Tyr-547, Ser-630, and Tyr-662, thereby inhibiting enzyme activity. Analysis of transmembrane transport pathway suggested that ILAPPER is transported by the Caco-2 cell monolayer via the paracellular pathway. All the results provide a new approach for rapid preparation of natural PkDPP-IV, and the potential application of ILAPPER as an antihyperglycemic peptide in functional foods.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140047"},"PeriodicalIF":7.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive review of plant small signaling peptides: From stress adaptation mechanisms to practical solutions for crop resilience.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-16 DOI: 10.1016/j.ijbiomac.2025.139971

Saiwen Chang, Fei Xiao

{"title":"Comprehensive review of plant small signaling peptides: From stress adaptation mechanisms to practical solutions for crop resilience.","authors":"Saiwen Chang, Fei Xiao","doi":"10.1016/j.ijbiomac.2025.139971","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139971","url":null,"abstract":"Small signaling peptides (SSPs), short proteins of fewer than 100 amino acids, serve as pivotal signaling molecules with diverse structural features, post-translational modifications, and functional roles. They regulate various aspects of plant growth and development by modulating specific cellular signaling pathways. Research has shown that many SSPs are essential for mediating responses to environmental stresses. This review presents the structure, characteristics, and classification of plant SSPs and elucidates their roles in resistance signaling pathways through interactions with their specific receptors. We then summarize recent findings on the biological functions and regulatory mechanisms of SSPs in response to both biotic and abiotic stresses. Finally, we discuss the potential applications and future prospects of these peptides in plant protection. This review offers valuable insights for enhancing plant resilience to environmental stress and advancing sustainable agricultural practices, while also providing key references and perspectives to accelerate research on SSPs in plants.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139971"},"PeriodicalIF":7.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal-organic cages based catalytic hybrid hydrogels for enhanced wound healing: Antibacterial and regenerative effects of Zr-MOC/chitosan composites hydrogel.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-16 DOI: 10.1016/j.ijbiomac.2025.139851

Jie Song, Haozhi Sun, Lixia Pan, Dandan Wang, Jinjun Wang, Feng Su

{"title":"Metal-organic cages based catalytic hybrid hydrogels for enhanced wound healing: Antibacterial and regenerative effects of Zr-MOC/chitosan composites hydrogel.","authors":"Jie Song, Haozhi Sun, Lixia Pan, Dandan Wang, Jinjun Wang, Feng Su","doi":"10.1016/j.ijbiomac.2025.139851","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139851","url":null,"abstract":"Metal-organic cages (MOCs), assembled by the coordination of metal nodes with organic ligands, offer excellent solvent dispersion, functionalization potential, and abundant binding sites, making them ideal for hybrid hydrogel synthesis. Herrin, a novel Zr-MOC/CS hybrid hydrogel was developed by crosslinking Zr-based metal-organic cages (Zr-MOC) and chitosan (CS) using dibenzaldehyde-functionalized polyethylene glycol (DF-PEG) as crosslinker, marking the first instance of incorporating Zr-MOC into a hydrogel matrix. The composite hydrogel leverages the catalytic activity of Zr-MOC to convert trace H2O2 into hydroxyl radicals (·OH), delivering enhanced antibacterial performance. Characterization via XRD, FT-IR, XPS, SEM and SEM-EDS confirmed the successful integration of Zr-MOC within the hydrogel matrix. Antibacterial assays demonstrated superior efficacy against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) compared to conventional hydrogels. Cytotoxicity tests (MTT and live-dead staining) confirmed excellent biocompatibility. Furthermore, in vivo experiments using an infected wound model revealed that the Zr-MOC/CS hydrogel significantly accelerated wound healing. These results highlight the potential of Zr-MOC/CS hydrogel as a multifunctional wound dressing material for antibacterial therapy in clinical applications.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139851"},"PeriodicalIF":7.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laccase immobilized on reduced graphene oxide sponges for simultaneous adsorption and enzymatic degradation of endocrine disrupting chemicals.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-16 DOI: 10.1016/j.ijbiomac.2025.139984

Mahsa Moayedi, Nariman Yousefi

{"title":"Laccase immobilized on reduced graphene oxide sponges for simultaneous adsorption and enzymatic degradation of endocrine disrupting chemicals.","authors":"Mahsa Moayedi, Nariman Yousefi","doi":"10.1016/j.ijbiomac.2025.139984","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139984","url":null,"abstract":"The presence of endocrine disrupting chemicals (EDCs) in water can impart detrimental effects on public health by mimicking the behaviors of natural hormones and their associated receptors in human body. Studies have demonstrated that ligninolytic enzymes such as laccase can degrade various phenolic compounds, including a broad range of EDCs. In this study, the technique of covalent immobilization of laccase through carbodiimide coupling chemistry on highly adsorptive reduced graphene oxide (rGO) sponges was utilized to effectively remove two representative EDCs; namely, bisphenol A (BPA) and triclosan (TCS) from water. The bio-functionalized adsorbent (rGO-LA) showed a significant improvement in removing BPA (87 % after 24 h) compared to pristine rGO sponge, (~40 % after 24 h). The removal efficiency of both adsorbents for TCS was as high as 84 %, with faster kinetics being observed for rGO-LA. Further investigation using gas-chromatography-mass spectroscopy revealed that the bio-functionalization not only improved the removal efficiency of the adsorbent, but also facilitated the adsorption of the metabolites generated during the biodegradation of BPA and TCS. When temperature was increased to 40 °C, the removal efficiency and kinetics of rGO-LA sponges were improved significantly for BPA (83 % removal in 4 h) and TCS (73 % removal after 4 h). The study highlights the synergy of enzymatic degradation and adsorption, with enhanced performance observed at elevated temperatures, offering a promising solution for effective EDCs mitigation in water treatment, while also ensuring comprehensive contaminant removal by adsorbing the generated metabolites.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139984"},"PeriodicalIF":7.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0