International Journal of Biological Macromolecules最新文献_第9页

Active packaging film based on chitosan/gelatin blend incorporated with mango peel carbon dots: Properties and shelf life extension of minced pork. 壳聚糖/明胶混合芒果皮碳点的活性包装膜：肉末的性能和保质期延长。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-11 DOI: 10.1016/j.ijbiomac.2024.138692

Arunachalasivamani Ponnusamy, Ajahar Khan, Thummanoon Prodpran, Jun Tae Kim, Soottawat Benjakul, Jong-Whan Rhim

{"title":"Active packaging film based on chitosan/gelatin blend incorporated with mango peel carbon dots: Properties and shelf life extension of minced pork.","authors":"Arunachalasivamani Ponnusamy, Ajahar Khan, Thummanoon Prodpran, Jun Tae Kim, Soottawat Benjakul, Jong-Whan Rhim","doi":"10.1016/j.ijbiomac.2024.138692","DOIUrl":"10.1016/j.ijbiomac.2024.138692","url":null,"abstract":"Active packaging is essential for reducing food quality loss and ensuring consumer safety. Recently, carbon dots, synthesized from agricultural bio-wastes, have been used as active nanofillers. Mango peels, generally discarded as waste, can serve as potential precursor for synthesis of carbon dots. Mango peel carbon dots (MPCD) were prepared and characterized. Characteristics of active film based on chitosan (CS)/fish gelatin (FG) blend incorporated with MPCD at different concentrations (1, 3, and 5 wt%) were investigated. MPCD with augmenting concentrations enhanced mechanical properties of CS/FG film. Film containing 5 % MPCD had 15 % higher tensile strength than the control (without MPCD). The film containing MPCD showed the improved antioxidant activity, antimicrobial and UV barrier properties. The pouch (5 × 5 cm2) made from film added with 5 % MPCD via heat sealing was used for packaging minced pork. Minced pork packed in the pouch showed lower bacterial growth (below 6 log CFU/g) and chemical changes than that packed in polyethylene pouch during 15 days of storage at 4 °C. Therefore, the conversion of mango peel into valuable carbon dots promotes a zero-waste sustainable approach in line with the biocircular economy. Active pouch could be employed as novel biodegradable active and green packaging for the food industry.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138692"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication, modification, interaction mechanisms, and applications of fish gelatin: A comprehensive review. 鱼明胶的制造、改性、相互作用机制和应用：综合综述。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-11 DOI: 10.1016/j.ijbiomac.2024.138723

Xian-Ni Su, Muhammad Fahad Khan, Xin-Ai, Dan-Lei Liu, Xiao-Fei Liu, Qiao-Li Zhao, Kit-Leong Cheong, Sai-Yi Zhong, Rui Li

{"title":"Fabrication, modification, interaction mechanisms, and applications of fish gelatin: A comprehensive review.","authors":"Xian-Ni Su, Muhammad Fahad Khan, Xin-Ai, Dan-Lei Liu, Xiao-Fei Liu, Qiao-Li Zhao, Kit-Leong Cheong, Sai-Yi Zhong, Rui Li","doi":"10.1016/j.ijbiomac.2024.138723","DOIUrl":"10.1016/j.ijbiomac.2024.138723","url":null,"abstract":"Fish gelatin (FG) is an essential natural biopolymer isolated from aquatic sources and has been considered as a feasible substitute for mammalian gelatins. However, its inferior mechanical and gelling properties limit its applications. Consequently, FG has been modified using various methods. This review summarizes the extraction techniques (including traditional acid and alkaline methods, as well as newer technologies such as ultrasonic-assisted and microwave-assisted extraction), modification strategies (mechanical treatments, physical mixing with polysaccharides, utilization of the Hofmeister effect, chemical modifications, etc.), along with their mechanisms of action. Additionally, we discussed the applications of FG and its modified products. Furthermore, this review highlights the safety and prospects for FG and its derivatives. The mechanical properties and biological functions of FGs are enhanced after modification. Thus, modified FG composites exhibit diverse applications in areas such as foaming agents and emulsifiers, food packaging, three-dimensional printing, drug delivery systems and tissue engineering. This paper aims to provide comprehensive information for future research on FG with the intention of broadening its applicability within the industries of food, cosmetics, and pharmaceuticals. Nevertheless, the development of tough gels, aerogels, and stimuli-responsive hydrogels based on FG requires further investigation.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138723"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fenugreek gum improves the rheological properties of konjac glucomannan in dynamic simulated digestion system and delays its gastric emptying. 葫芦巴胶改善魔芋葡甘露聚糖在动态模拟消化系统中的流变特性，延缓其胃排空。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-11 DOI: 10.1016/j.ijbiomac.2024.138713

Wenjing Chen, Ding An, Shuxin Ye, Sha Li, Jing Li, Bin Li

{"title":"Fenugreek gum improves the rheological properties of konjac glucomannan in dynamic simulated digestion system and delays its gastric emptying.","authors":"Wenjing Chen, Ding An, Shuxin Ye, Sha Li, Jing Li, Bin Li","doi":"10.1016/j.ijbiomac.2024.138713","DOIUrl":"10.1016/j.ijbiomac.2024.138713","url":null,"abstract":"The physicochemical properties of konjac glucomannan (KGM) are impaired in the harsh gastrointestinal tract, which may reduce its effectiveness in physiological functions. In this paper, fenugreek gum (FG) with high water holding capacity and stability was used as a gastric protectant for KGM, and the effects of the KGM-FG complexes with different composite ratios on gastric emptying were researched by in vitro dynamic simulated gastric digestion system. The results showed that FG significantly enhanced the delayed gastric emptying properties of KGM. Adding FG reduced the apparent viscosity, flow behavior, and mechanical properties of KGM. The simulated gastric fluid (SGF) decreased the apparent viscosity of the KGM-FG complex and increased the microstructure network density of the KGM-FG complex compared with the water system. FG helped the structure of the KGM-FG complexes become more stable and trapped more water in the stomach. The KGM-FG complex with high viscosity, mechanical modulus, and frictional resistance in a dynamic simulated digestion system increased gastric retention. The KGM-FG complex with a composite ratio 5:5 showed the best performance and a potential satiety-enhancing property. The results provided a theoretical basis for designing satiety food formulations that help control energy intake and body weight.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138713"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the relationship between cell wall, intracellular starch structure and physicochemical properties of cassava cells from cassava varieties. 木薯品种细胞壁、胞内淀粉结构与木薯细胞理化性质关系的研究。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-11 DOI: 10.1016/j.ijbiomac.2024.138728

Meng Jia, Rongrong Ma, Chang Liu, Xiaohua Pan, Wangyang Shen, Yaoqi Tian

{"title":"Insight into the relationship between cell wall, intracellular starch structure and physicochemical properties of cassava cells from cassava varieties.","authors":"Meng Jia, Rongrong Ma, Chang Liu, Xiaohua Pan, Wangyang Shen, Yaoqi Tian","doi":"10.1016/j.ijbiomac.2024.138728","DOIUrl":"10.1016/j.ijbiomac.2024.138728","url":null,"abstract":"Cassava cell flour can expand the food industrial availability of cassava resources. In this study, cassava cells were isolated from eight cassava varieties to analyze the composition, structure, and physicochemical properties. The smaller particle size in CS4 led to the lowest swelling power and viscosity, which further reduced the modulus (G', G\") and shear stress of the cassava cell gel. The higher starch content in CS3 and CS8 (88.86 %, 87.99 %) increased the swelling and solubility of the cells, resulting in high viscosity and gel strength. Thicker cell walls presented a higher content of cell wall polysaccharides, hindering the interaction of water, heat, and digestive enzymes with intracellular starch, leading to higher gelatinization temperatures and lower digestion rates. In addition, the B1 chain promoted the formation of the starch crystalline region and increased the gelatinization temperature and enthalpy of cell flour. Based on cluster analysis and correlation analysis, the differences in the functional properties of cassava cell flour were related to cell components, morphology, and intracellular starch structure. The study provided a theoretical basis for the application of cassava cells in the food industry.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138728"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic landscapes of STING-mediated DNA-sensing reveal cellular response heterogeneity. STING 介导的 DNA 感测的转录组图谱揭示了细胞反应的异质性。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-13 DOI: 10.1016/j.ijbiomac.2024.138752

Ling-Zu Kong, In-Hwan Jang, Chunli Wang, Soo Yun Lee, Seok-Min Kim, Se-Chan Oh, Sunyoung Lee, Seona Jo, Ji Hyun Kim, Kee K Kim, Tae-Don Kim

{"title":"Transcriptomic landscapes of STING-mediated DNA-sensing reveal cellular response heterogeneity.","authors":"Ling-Zu Kong, In-Hwan Jang, Chunli Wang, Soo Yun Lee, Seok-Min Kim, Se-Chan Oh, Sunyoung Lee, Seona Jo, Ji Hyun Kim, Kee K Kim, Tae-Don Kim","doi":"10.1016/j.ijbiomac.2024.138752","DOIUrl":"10.1016/j.ijbiomac.2024.138752","url":null,"abstract":"Transfection of plasmid DNA (pDNA) encoding target genes is a routine tool in gene function studies and therapeutic applications. However, nucleic acid-sensing-mediated innate immune responses influence multiple intracellular signaling pathways. The stimulator of interferon genes (STING) is a crucial adapter protein for DNA sensors in mammalian cells. In this study, we explored the molecular mechanisms underlying DNA sensing by investigating the relationship between mRNA and protein expression levels and the STING pathway using single-cell analysis. We observed that reporter gene expression was dose-nonlinear after transfection of pDNA in cells with intact DNA-sensing pathways. Moreover, blocking the STING pathway in THP-1 cells significantly downregulated innate immune responses, upregulated exogenous gene expression, and mitigated the effects of innate immune responses on cell and gene function, but did not affect the proportion of reporter protein-positive cells. We elucidated the mechanisms of DNA sensing-induced innate immune response and cell death by analyzing heterozygous cellular responses to DNA transfection and transcriptome changes in positive cells. These findings suggest that the regulation of STING-mediated nucleic acid-sensing pathways is crucial for the accuracy of gene function studies and could enhance the efficacy of gene therapy.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138752"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering adsorption behaviour and mechanisms of enhanced phosphate removal via optimized cetyltrimethylammonium bromide-modified nanofibrillated cellulose. 十六烷基三甲基溴化铵改性纳米纤化纤维素增强磷酸盐去除的吸附行为和机理。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-16 DOI: 10.1016/j.ijbiomac.2024.138743

Archana Pandey, Ajay S Kalamdhad, Yogesh Chandra Sharma

{"title":"Deciphering adsorption behaviour and mechanisms of enhanced phosphate removal via optimized cetyltrimethylammonium bromide-modified nanofibrillated cellulose.","authors":"Archana Pandey, Ajay S Kalamdhad, Yogesh Chandra Sharma","doi":"10.1016/j.ijbiomac.2024.138743","DOIUrl":"10.1016/j.ijbiomac.2024.138743","url":null,"abstract":"To combat the persistent environmental issues resulting from eutrophication, it is necessary to scavenge excess phosphorous levels from aquatic ecosystems. In response, a cationic adsorbent was prepared by modifying agrowaste-derived natural biomacromolecule; nanofibrillated cellulose (NFC) using cetyltrimethylammonium bromide (CTAB) surfactant. Comprehensive characterization through XRD, FTIR, HR-SEM, SEM-EDX, BET and XPS demonstrated that quaternizing NFC significantly improved its surface chemistry by introducing substantial quaternary ammonium groups. This modification imparted positive ζ potential across broad pH range, underscoring a strong affinity for negatively charged phosphate ions. Enhanced roughness and improved spatial dispersion led to nearly threefold increase in phosphate removal efficiency compared to pristine NFC, attributable to a higher number of available active sites. The adsorption process followed pseudo-second-order kinetic and Sips isotherm model, with a maximum adsorption capacity of 21.78 mg P/g, reaching equilibrium within 120 min. Besides, the prepared adsorbent demonstrated pH-dependent adsorption and displayed stable adsorption capacity particularly at weakly acidic or neutral pH conditions. Furthermore, it exhibited excellent retention capacity with only 12.61 % desorption rates over three cycles. Both XPS and FTIR results revealed that electrostatic adsorption (based on Lewis acid-base principle) and hydrogen bonding were primary adsorption mechanisms.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138743"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Etched CoFe Prussian blue analog nanozymes with superior peroxidase activity for colorimetric biosensing of hydrogen peroxide and dopamine. 具有优异过氧化物酶活性的蚀刻咖啡普鲁士蓝模拟纳米酶，用于过氧化氢和多巴胺的比色生物传感。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-12 DOI: 10.1016/j.ijbiomac.2024.138766

N Ch Ramgopal, Poulami Mukherjee, Tapas Kamilya, Nagaraj Basavegowda, Mallappa Mahanthappa, Nada H Aljarba, Reem A Alqahtani, Saad Alkahtani, Jinhyoung Park, R S Vishwanath

{"title":"Etched CoFe Prussian blue analog nanozymes with superior peroxidase activity for colorimetric biosensing of hydrogen peroxide and dopamine.","authors":"N Ch Ramgopal, Poulami Mukherjee, Tapas Kamilya, Nagaraj Basavegowda, Mallappa Mahanthappa, Nada H Aljarba, Reem A Alqahtani, Saad Alkahtani, Jinhyoung Park, R S Vishwanath","doi":"10.1016/j.ijbiomac.2024.138766","DOIUrl":"10.1016/j.ijbiomac.2024.138766","url":null,"abstract":"Nanozymes represent a compelling alternative to natural enzymes due to their exceptional stability and cost-efficiency in diagnostic and therapeutic applications. In this work, a nanozyme etched CoFe Prussian blue analog (etched PBA) was designed and fabricated by a simple approach involving first synthesizing CoFe PBA and then chemical etching. The fabricated etched PBA acts as a peroxidase mimic catalyst. Compared to the naturally occurring enzyme (horseradish peroxidase), the etched PBA exhibited improved peroxidase-like catalytic efficiency for oxidizing 3,3',5,5'-tetramethylbnmenzidine (TMB). Particularly, the exceptional peroxidase-like activity of the etched PBA is attributed to modifications in its electronic and structural properties, which improved affinity for hydrogen peroxide (H2O2) and TMB. Hence, these findings highlight etched PBA as a promising peroxidase mimic, enabling a colorimetric assay for real-time H2O2 and dopamine (DA) detection. H2O2 was detected via PBA-catalyzed TMB oxidation (bluish-green color), while DA monitoring occurred through ox-TMB reduction. The detection thresholds for H2O2 and DA were determined to be 0.083 μM and 0.05 μM, respectively, with broad linear ranges of 0.1 μM - 4.0 mM for H2O2 and 0.1-240 μM for DA. In addition, the etched PBA was validated for real sample analysis, showing potential for enzyme catalysis, biosensing, and colorimetric analysis of pharmaceuticals and biomolecules.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138766"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genipin-enriched chitosan-Zein nanoparticles for improved curcumin encapsulation. 用于改善姜黄素包囊的富含 Genipin 的壳聚糖-玉米蛋白纳米颗粒。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-12 DOI: 10.1016/j.ijbiomac.2024.138555

Lingling Sha, Husnain Raza, Chengsheng Jia, Imran Mahmood Khan, Huqing Yang, Gang Chen

{"title":"Genipin-enriched chitosan-Zein nanoparticles for improved curcumin encapsulation.","authors":"Lingling Sha, Husnain Raza, Chengsheng Jia, Imran Mahmood Khan, Huqing Yang, Gang Chen","doi":"10.1016/j.ijbiomac.2024.138555","DOIUrl":"10.1016/j.ijbiomac.2024.138555","url":null,"abstract":"Polysaccharide-protein nanocomplexes are considered one of the desired systems to encapsulate bioactive compounds. The study prepared chitosan (CS)/genipin (GP)/zein nanoparticles with chemical cross-linkage for encapsulating and releasing curcumin. Fourier transform infrared spectra demonstrated that cross-linkage between molecules was attributed to electrostatic interactions and the formation of amido bonds and hydrogen bonding. Transmission electron microscopy showed that all prepared nanoparticles showed a spherical morphology. Particularly, cinnamaldehyde screening -NH2 groups in CS significantly reduced the encapsulation efficiency of nanoparticles, demonstrating the significant role of the -NH2 group in CS-coated zein nanoparticles. Additionally, the encapsulation efficiency was largely increased to 82.3 % at the ratio of CS/GP/zein (w/w, 20:1:100), compared with 38.4 % using zein as a single encapsulation for curcumin. In vitro digestion, the addition of GP and CS decreased the release of curcumin from 84.8 % to 57.1 % after the initial digestion of 2 h. The GP/CS/zein particles retained 12 % of curcumin after 6- h of digestion, indicating the improvements in encapsulation efficiency and release properties.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138555"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Nrf2 suppression and Camkk1 upregulation in Echinococcus granulosus-induced bone loss. 棘球蚴肉芽肿诱发骨质流失的 Nrf2 抑制和 Camkk1 上调机制

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-12 DOI: 10.1016/j.ijbiomac.2024.138521

Yansheng Huang, Yiping Huang, Jun Xiao, Yibo Ma, Yaqing Liu, Haohao Sun, Yi Dai, Qian Ren, Sibo Wang

{"title":"Mechanisms of Nrf2 suppression and Camkk1 upregulation in Echinococcus granulosus-induced bone loss.","authors":"Yansheng Huang, Yiping Huang, Jun Xiao, Yibo Ma, Yaqing Liu, Haohao Sun, Yi Dai, Qian Ren, Sibo Wang","doi":"10.1016/j.ijbiomac.2024.138521","DOIUrl":"10.1016/j.ijbiomac.2024.138521","url":null,"abstract":"Osteoclast differentiation is essential for maintaining bone metabolism, and its dysregulation, particularly in the context of Echinococcus granulosus (CE) infection, can lead to severe bone loss. This study explores a novel mechanism by which CE protoscolices (PSC) drive osteoclast differentiation through the inhibition of Nrf2, followed by the upregulation of Camkk1. Transcriptome sequencing revealed a significant down-regulation of Nrf2 in cells treated with PSC. This was confirmed by Western blot and Q-PCR assays showing reduced Nrf2 protein and gene levels. In vivo studies with Nrf2 knockout mice demonstrated that the absence of Nrf2 exacerbates bone loss induced by PSC in both the spine and lower limbs, as observed through Micro-CT imaging and TRAP staining.Further investigations identified Camkk1 as a key downstream target of Nrf2. Using high-throughput sequencing and CO-IP experiments, we established that Nrf2 directly interacts with and regulates Camkk1. Functional assays indicated that PSC-induced upregulation of Camkk1 is significantly enhanced by Nrf2 knockdown, while silencing Camkk1 alone inhibits osteoclast differentiation.The therapeutic potential of this pathway was evaluated by screening small molecule inhibitors of Camkk1, with Crenolani emerging as a potent compound. In vivo administration of Crenolani in PSC-treated mice significantly alleviated bone loss in a dose-dependent manner.These findings elucidate a crucial molecular mechanism in osteoclast differentiation driven by CE infection and propose a promising therapeutic strategy for combating CE-induced bone destruction. This study advances our understanding of bone.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138521"},"PeriodicalIF":7.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Preparation, characterization, stability and application of the H-type aggregates lutein/whey protein/chitosan nanoparticles" [Int. J. Biol. Macromol., volume 282, part 32, page 136739]. h型叶黄素/乳清蛋白/壳聚糖纳米颗粒聚集体的制备、表征、稳定性和应用[j]。生物。絮凝。[第282卷，第32部分，第136739页]。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-02-01 Epub Date: 2024-12-17 DOI: 10.1016/j.ijbiomac.2024.138563

Yuting Sun, Yingyuan Zhao, Mengyue Qiu, Ye Zhang, Jin Liang, Shiying Xie, Ruifang Li, Xueqin Wang

引用次数: 0