International Journal of Biological Macromolecules最新文献_第9页

Multi-modal antibacterial lignin-based nanocomposites with photothermal and free radical therapy for bacterial infection treatment 多模态抗菌木质素基纳米复合材料光热自由基治疗细菌感染。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145191

Si-Man Luo , Cong-Min Huo , Liheng Chen , Xiao-Nan Li , Rui Huang , Zhi-Sheng Ji , Hong Zhang , Wei Xue , Jing-Yi Zhu

{"title":"Multi-modal antibacterial lignin-based nanocomposites with photothermal and free radical therapy for bacterial infection treatment","authors":"Si-Man Luo , Cong-Min Huo , Liheng Chen , Xiao-Nan Li , Rui Huang , Zhi-Sheng Ji , Hong Zhang , Wei Xue , Jing-Yi Zhu","doi":"10.1016/j.ijbiomac.2025.145191","DOIUrl":"10.1016/j.ijbiomac.2025.145191","url":null,"abstract":"<div><div>Bacterial infections remain a serious global health concern, exacerbated by the rise of drug-resistant strains. In this study, we present a smart, thermo-responsive antibacterial nanocomposite that synergistically integrates photothermal therapy and free radical generation to effectively combat bacterial infections. Gold nanoparticles (AuNPs), stabilized by lignin, self-assemble into sea urchin-like LNPs-AuNPs with efficient photothermal conversion efficiency under near-infrared (NIR) irradiation. The incorporation of azo compound AIPH enables the release of oxygen-independent alkyl radicals upon heating, further enhancing its antibacterial effect. Both <em>in vitro</em> assays and <em>in vivo</em> experiments, including a rat skin infection model, demonstrated that the LNPs-Au/AIPH effectively eradicate <em>S. aureus</em> and <em>E. coli</em>, reduce inflammation, and accelerate tissue healing. This dual-mode nanoplatform offers a promising antibiotic-free strategy for combating bacterial infections, with enhanced therapeutic efficacy and minimal side effects.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145191"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of CYP9J26, a cytochrome P450 associated with insecticide resistance in the arbovirus vector Aedes albopictus 虫媒病毒载体白纹伊蚊中与杀虫剂抗性相关的细胞色素P450 CYP9J26的功能特征

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145280

Yuhan Zhao , Xiaojie Zhou , Mei Li , Xinghui Qiu

{"title":"Functional characterization of CYP9J26, a cytochrome P450 associated with insecticide resistance in the arbovirus vector Aedes albopictus","authors":"Yuhan Zhao , Xiaojie Zhou , Mei Li , Xinghui Qiu","doi":"10.1016/j.ijbiomac.2025.145280","DOIUrl":"10.1016/j.ijbiomac.2025.145280","url":null,"abstract":"<div><div><em>Aedes albopictus</em> is a primary vector of arboviral diseases such as dengue fever, chikungunya fever, and Zika. The rapid development of insecticide resistance is becoming a major challenge to sustainability of insecticide-based interventions for the control of <em>Ae. albopictus</em> borne arboviral diseases. Understanding the molecular basis of resistance is useful for developing effective insecticide-based control action. Our recent study showed that overexpression of the <em>AalbCYP9J26</em> gene was associated with insecticide resistance in field <em>Ae. albopictus</em> populations in Beijing, but its contribution to insecticide metabolism and resistance remains unknown. In this study, the metabolic capacity of AalbCYP9J26 towards six insecticides was examined using an enzyme system reconstituted with <em>Escherichia coli</em>-produced AalbCYP9J26 protein and its redox partners (AalbCPR and Aalbb5). The results showed that AalbCYP9J26 was able to metabolize temephos, malathion, propoxur, permethrin, and deltamethrin, while no significant activity against bendiocarb was observed. Moreover, the <em>Drosophila</em> GAL4/UAS transgenic system confirmed that enhanced expression of AalbCYP9J26 conferred increased tolerance to malathion, permethrin, deltamethrin, and propoxur. This study demonstrates that AalbCYP9J26 plays a critical role in insecticide metabolism and resistance, suggesting AalbCYP9J26 may serve as a valuable biomarker for monitoring insecticide resistance and a target for new insecticidal agents.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145280"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poly-γ-glutamic acid alleviates cytotoxicity and inflammation induced by pre-formed fibrils of α-synuclein in murine primary astrocytes 聚γ-谷氨酸可减轻小鼠初代星形胶质细胞α-突触核蛋白原纤维诱导的细胞毒性和炎症反应

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145303

Claudia Novello , Mattia Parati , Samanta Mazzetti , Oriana Rampoldi , Huseyin Berkcan Isilgan , Milo Jarno Basellini , Chiara M.G. De Luca , Arianna Ciullini , Ilaria L. Dellarole , Alessandro Fantin , Isabella Russo , Brian L. Johnston , Moira Paroni , Chiara Rolando , Fabio Moda , Gianni Pezzoli , Iza K. Radecka , Graziella Cappelletti

{"title":"Poly-γ-glutamic acid alleviates cytotoxicity and inflammation induced by pre-formed fibrils of α-synuclein in murine primary astrocytes","authors":"Claudia Novello , Mattia Parati , Samanta Mazzetti , Oriana Rampoldi , Huseyin Berkcan Isilgan , Milo Jarno Basellini , Chiara M.G. De Luca , Arianna Ciullini , Ilaria L. Dellarole , Alessandro Fantin , Isabella Russo , Brian L. Johnston , Moira Paroni , Chiara Rolando , Fabio Moda , Gianni Pezzoli , Iza K. Radecka , Graziella Cappelletti","doi":"10.1016/j.ijbiomac.2025.145303","DOIUrl":"10.1016/j.ijbiomac.2025.145303","url":null,"abstract":"<div><div>Poly-γ-glutamic acid (γ-PGA) is a bacterial-derived natural biopolymer that has gathered significant interest due to its antioxidant, anti-inflammatory, and neuroprotective properties. These characteristics make γ-PGA a potential candidate for the treatment of neurodegenerative diseases. In Parkinson's disease (PD), whose key pathological feature is the accumulation of neuronal α-synuclein aggregates, astrocytes, in addition to microglia, play a crucial role in clearing these aggregates; however, their capacity is limited. Overwhelmed astrocytes trigger an inflammatory response that exacerbates neurodegeneration. Therefore, strategies aimed at regulating the uptake of extracellular α-synuclein aggregates by astrocytes and mitigating inflammation could hold therapeutic promise. This work aimed to investigate the potential of γ-PGA in preventing or reversing the toxicity and inflammatory response induced by pre-formed α-synuclein fibrils (PFFs) in murine cortical astrocytes. Cell viability assays demonstrated that γ-PGA can counteract the toxicity induced by α-synuclein PFFs. Confocal microscopy and 3D reconstruction analyses revealed that γ-PGA colocalizes with PFFs, leading to a reduction in the uptake of these aggregates by astrocytes and a subsequent decrease in their inflammatory response. Consequently, γ-PGA emerges as a promising candidate for further investigation in the therapeutic management of PD.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145303"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization of N-glycans on human transthyretin and their impact on amyloid-β binding n -聚糖对人甲状腺转甲素的结构表征及其对淀粉样蛋白-β结合的影响。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145286

Han Seul Lee , Jieun Kim , Chi Soo Park, Chulmin Moon, Chang Myeong Jeong, Kyuran Kim, Haeun Byeon, Daeun Eom, Ha Hyung Kim

{"title":"Structural characterization of N-glycans on human transthyretin and their impact on amyloid-β binding","authors":"Han Seul Lee , Jieun Kim , Chi Soo Park, Chulmin Moon, Chang Myeong Jeong, Kyuran Kim, Haeun Byeon, Daeun Eom, Ha Hyung Kim","doi":"10.1016/j.ijbiomac.2025.145286","DOIUrl":"10.1016/j.ijbiomac.2025.145286","url":null,"abstract":"<div><div>Transthyretin (TTR), a plasma and cerebrospinal fluid protein, binds amyloid-β (Aβ) peptides, inhibiting their aggregation and amyloid fibril formation—key processes implicated in Alzheimer's disease pathogenesis. Despite its critical functions, the structural characterization of TTR remains incomplete. In this study, liquid chromatography-tandem mass spectrometry was employed to identify and characterize the <em>N</em>-glycans on human TTR and evaluate their role in Aβ binding. A total of 18 <em>N</em>-glycan structures were identified, comprising high-mannose (2.1 %), hybrid (0.4 %), and complex (97.5 %) types, with a total <em>N</em>-glycan quantity of 0.22 pmol/pmol TTR. The <em>N</em>-glycan modifications included galactosylation (96.0 %), fucosylation (26.8 %), mono-sialylation (30.1 %), and bisected GlcNAc (5.5 %). Proteolytic peptide analysis revealed partial <em>N</em>-glycosylation at Asn-98 with an occupancy of 16.5 %. Functional assays showed that desialylation and complete deglycosylation resulted in reduced Aβ binding, as assessed by ELISA, and reduced Aβ-aggregation inhibition by thioflavin T assay, compared to intact TTR. These results indicate that, despite its low occupancy (4.9 %, with 30.1 % sialylation at the 16.5 % occupied site) at Asn-98, negatively charged sialylation plays an important role in the Aβ binding capacity of TTR. This study provides the first detailed characterization of human TTR <em>N</em>-glycans and highlights their essential role in the binding of TTR to Aβ.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"319 ","pages":"Article 145286"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a novel system for the expression and production of recombinant monoclonal antibody, tocilizumab, using Pichia pastoris 利用毕赤酵母表达和生产重组单克隆抗体tocilizumab的新系统的开发

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145297

Prabir Kumar Das, Ansuman Sahoo, Venkata Dasu Veeranki

{"title":"Development of a novel system for the expression and production of recombinant monoclonal antibody, tocilizumab, using Pichia pastoris","authors":"Prabir Kumar Das, Ansuman Sahoo, Venkata Dasu Veeranki","doi":"10.1016/j.ijbiomac.2025.145297","DOIUrl":"10.1016/j.ijbiomac.2025.145297","url":null,"abstract":"<div><div>The development of anti-interleukin-6-receptor (IL6R) recombinant monoclonal antibody Tocilizumab (TCZ) production in a methylotrophic yeast <em>Pichia pastoris</em> was the focus of this research work. The molecular weight of full-length TCZ is approximately 148 kDa. A bicistronic expression vector with both heavy and light chain genes was constructed within individual inducible alcohol oxidase promoters and secretory signal peptides. The recombinant strains were subcultured several times to maintain the stability of both heterologous antibody genes. A gene copy number ratio (light to heavy chain) of 3:2 was observed to be the best transformant for the secretory expression of Tocilizumab. The expression of TCZ was confirmed with Western blot and ELISA techniques. The structural analysis of full-length TCZ was carried out by circular dichroism spectroscopy and alpha fold predictions. The extracellular expression of the full-length TCZ is achieved at ~0.33 mg/L over 96 h of batch fermentation. The antibody was further purified using Protein A resin-based affinity chromatography, and various comparative cell-free and cell-based functional assays were performed with marketed TCZ (ACTEMRA™, Cipla) and <em>Pichia</em>-derived TCZ to establish the interaction with its cognate receptor IL6R and functional efficacy, respectively.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145297"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of chitosan-based films enhanced with vanillin-based deep eutectic solvents (V-DEA) for prolonging litchi shelf life and postharvest quality. 香兰素基深共晶溶剂（V-DEA）增强壳聚糖基薄膜的研制，以延长荔枝的保质期和采后品质。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145144

Hafiz Umer Javed, Jiechang Lai, Jiaxiu Du, Sixia Zhong, Huiquan Wu, Weiying Jiang, Ming He, Jingqi Shang, Mengmeng Zhang, Sajid Ali, Yuan-Sen Liu, Wang Xin, Huimin Yao, Dehong Lu, Xugang Shu, Li-Yan Zeng

{"title":"Development of chitosan-based films enhanced with vanillin-based deep eutectic solvents (V-DEA) for prolonging litchi shelf life and postharvest quality.","authors":"Hafiz Umer Javed, Jiechang Lai, Jiaxiu Du, Sixia Zhong, Huiquan Wu, Weiying Jiang, Ming He, Jingqi Shang, Mengmeng Zhang, Sajid Ali, Yuan-Sen Liu, Wang Xin, Huimin Yao, Dehong Lu, Xugang Shu, Li-Yan Zeng","doi":"10.1016/j.ijbiomac.2025.145144","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145144","url":null,"abstract":"<p><p>This study developed a novel solid-state Vanillin-Based Deep Eutectic Agent (V-DEA) as a green plasticizer to enhance chitosan films for food packaging applications. The chemical structure of V-DEA film, confirmed via <sup>1</sup>H NMR spectroscopy, showed successful integration into chitosan films. The incorporation of V-DEA significantly improved the films' mechanical strength (tensile strength up to 35 MPa), flexibility (elongation at break of 23 %), and thermal stability, with 60 % V-DEA identified as the optimal concentration. Morphological analysis revealed increased surface roughness and porosity at higher V-DEA levels, affecting barrier properties such as transparency and water vapor permeability. Antioxidant and antibacterial assays showed strong radical scavenging activity and effective inhibition against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), especially at 80 % V-DEA. Application on litchi fruits demonstrated significant preservation effects: reduced browning index, minimized weight loss, and maintenance of vitamin C and anthocyanin content over 5 days. These results highlight the potential of V-DEA/Ch films as multifunctional, bioactive, and biodegradable packaging materials for extending the shelf life and quality of perishable fruits. This work introduces a new class of bio-based plasticizers for active food packaging, combining environmental sustainability with enhanced postharvest functionality.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145144"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periodate oxidation of polysaccharides from Agaricus blazei edible mushroom and their toxicity in adult zebrafish (Denio rerio) 姬松茸多糖的高碘酸氧化及其对成年斑马鱼的毒性研究

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145294

Matheus Morais Saraiva , Elison Breno Alencar , Vitória Maria de Freitas Franco , Maria Vitoria Fonteles , Maria Leônia da Costa Gonzaga , José Eduardo Ribeiro Honório Júnior , Jeanlex Soares de Sousa , Sandra de Aguiar Soares , Nágila Maria Pontes Silva Ricardo , Maria Elenir Nobre Pinho Ribeiro

{"title":"Periodate oxidation of polysaccharides from Agaricus blazei edible mushroom and their toxicity in adult zebrafish (Denio rerio)","authors":"Matheus Morais Saraiva , Elison Breno Alencar , Vitória Maria de Freitas Franco , Maria Vitoria Fonteles , Maria Leônia da Costa Gonzaga , José Eduardo Ribeiro Honório Júnior , Jeanlex Soares de Sousa , Sandra de Aguiar Soares , Nágila Maria Pontes Silva Ricardo , Maria Elenir Nobre Pinho Ribeiro","doi":"10.1016/j.ijbiomac.2025.145294","DOIUrl":"10.1016/j.ijbiomac.2025.145294","url":null,"abstract":"<div><div>This study characterizes periodate-oxidized polysaccharides through FTIR, NMR, and thermogravimetric analysis, confirming successful oxidation with degrees ranging from 5.4 % to 36.3 % (theoretical degrees: 10 to 50 %). The materials exhibited reduced molecular weights (2.74 × 10<sup>6</sup> to 5.78 × 10<sup>5</sup> Da), negative zeta potentials (most pronounced in P50), decreased particle sizes, and pseudoplastic behavior. Zebrafish toxicity assessments revealed low acute toxicity (no significant mortality at 96 h, preventing IC₅₀ determination), though adverse effects including pigmentation changes, photophobia, and hyperactivity were observed. Open-field tests demonstrated CNS activity, with P0/P10 groups showing anxiolytic effects and P30/P50 exhibiting sedation. While the results confirm the materials' biocompatibility, the observed neurological effects warrant further investigation into their CNS interactions. The combination of low toxicity, and tunable rheological behavior suggests promising applications in food technology (particularly as safer alternatives to conventional crosslinkers) and biomaterial development. Future studies should focus on: elucidating structure-activity relationships governing CNS effects, optimizing oxidation protocols for specific applications, and evaluating performance in functional food and biomedical formulations. This work establishes oxidized polysaccharides as versatile biomaterials with dual potential as emulsifiers and bioactive carriers, while highlighting the need for targeted neuroactivity studies.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145294"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CCDesign and research of synergistic antibacterial composite materials based on MOF-stabilized enzymes and in situ grown AgNPs 基于mof稳定酶和原位生长AgNPs的协同抗菌复合材料的设计与研究。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145130

Zihao Yu , Yuan Shi , Jialiang Lin , Yan Wang , Chunlu He , Jianhua Cheng

{"title":"CCDesign and research of synergistic antibacterial composite materials based on MOF-stabilized enzymes and in situ grown AgNPs","authors":"Zihao Yu , Yuan Shi , Jialiang Lin , Yan Wang , Chunlu He , Jianhua Cheng","doi":"10.1016/j.ijbiomac.2025.145130","DOIUrl":"10.1016/j.ijbiomac.2025.145130","url":null,"abstract":"<div><div>Overusing antibiotics has accelerated the rapid emergence and spread of resistant strains, posing a serious threat to global public health. To address this challenge, this study developed a synergistic antibacterial composite material, CAT@ZIF-8/AgNPs, based on the metal-organic framework material ZIF-8, catalase (CAT), and silver nanoparticles (AgNPs). First, CAT was encapsulated in ZIF-8, effectively enhancing the enzyme's stability and catalytic activity in complex environments. AgNPs were then grown in situ on the surface of CAT@ZIF-8, which endowed the composite material with broad-spectrum antibacterial properties and synergistically enhanced the antibacterial effect through silver ion release and enzymatic catalysis. Enzyme activity tests demonstrated that CAT@ZIF-8 exhibited excellent stability, retaining over 50 % activity at pH 11, above 80 % at 50 °C, and over 70 % in organic solvents, significantly outperforming free CAT under all tested conditions. Antibacterial tests indicated that the composite material exhibited significant synergistic antibacterial effects against <em>Escherichia coli</em>, <em>Staphylococcus aureus</em>, and <em>Pseudomonas aeruginosa</em>. Furthermore, cytotoxicity tests revealed that the composite material demonstrated good biocompatibility with human keratinocytes (HaCaT) at concentrations up to 500 μg/mL. The CAT@ZIF-8/AgNPs composite material showcases efficient antibacterial performance and good biosafety through the synergistic action of enzymes and silver nanoparticles, indicating broad application prospects.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"319 ","pages":"Article 145130"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Electrospinning of recombinant human-like collagen-reinforced PCL nanofibrous membranes using benign solvents for periodontal regeneration" [Int. J. Biol. Macromol. 284 (Part 1) (January 2025) 137954]. “用良性溶剂静电纺丝重组人样胶原增强PCL纳米纤维膜用于牙周再生”的更正[j]。生物。Macromol. 284 (Part 1) (January 2025) 137954]。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145182

Minyi Ren, Meng Li, Aldo R Boccaccini, Yan Xu, Lu Li, Kai Zheng

引用次数: 0

Effects of fermented polyphenols from Lonicera caerulea on Streptococcus mutans pathogenicity: Exopolysaccharide structure and quorum sensing regulation 金银花发酵多酚对变形链球菌致病性的影响：胞外多糖结构和群体感应调节

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI: 10.1016/j.ijbiomac.2025.145265

Yanxin Ren , Xiaohang Zhou , Baiyan Cai , Yangcun Sun , Jingping Ge , Wenxiang Ping

{"title":"Effects of fermented polyphenols from Lonicera caerulea on Streptococcus mutans pathogenicity: Exopolysaccharide structure and quorum sensing regulation","authors":"Yanxin Ren , Xiaohang Zhou , Baiyan Cai , Yangcun Sun , Jingping Ge , Wenxiang Ping","doi":"10.1016/j.ijbiomac.2025.145265","DOIUrl":"10.1016/j.ijbiomac.2025.145265","url":null,"abstract":"<div><div>Dental caries, a common chronic oral disease, is mainly caused by cariogenic dental plaque biofilms. Current antibiotic therapies for preventing it have limitations, such as drug resistance and side effects. Polyphenols are bioactive plant compounds displaying potential in oral health. <em>Lonicera caerulea</em> polyphenols (LCP) have various physiological effects, yet research on their use in preventing and treating dental caries is limited. This study aimed to explore the impact of <em>L. caerulea</em>-fermented polyphenols on <em>Streptococcus mutans</em> and its mechanism of action on exopolysaccharides (EPS). The extracted and purified EPS of <em>S. mutans</em> were treated with LCP. LCP reduced the molecular weight (Mw) of EPS from 1.15 × 10<sup>5</sup> Da to 9.74 × 10<sup>4</sup> Da and increased the content of <em>β</em>-1,5-gal in EPS. Moreover, scanning electron microscopy showed that LCP treatment made EPS surface rough and porous, indicating that LCP disrupted the original dense structure of EPS. Fourier-transform infrared spectroscopy and nuclear magnetic resonance analyses further confirmed that LCP did not significantly alter the major functional groups of EPS but influenced the types of its glycosidic bonds. Meanwhile, LCP inhibited the growth of <em>S. mutans</em>, reduced the formation of EPS and biofilms, and suppressed the expression of genes related to quorum sensing (<em>luxS</em>) and biofilms (<em>brpA</em>). LCP exhibited a remarkable inhibitory effect on <em>S. mutans.</em> The findings indicated that LCP might serve as a natural preparation for preventing dental caries, thus providing a new research direction in the field of oral health.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145265"},"PeriodicalIF":7.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0