International Journal of Biological Macromolecules最新文献

{"title":"Preparation of photoluminescent transparent balsa wood with densifying structure for light-scattering windows based on cross-linking of lignocellulose and poly(vinyl alcohol)","authors":"Shoufu Long,&nbsp;Dingqiang Zheng,&nbsp;Ming Zhang,&nbsp;Wangchun Gou,&nbsp;Zhenghui Li,&nbsp;Dongsheng Song","doi":"10.1016/j.ijbiomac.2025.143639","DOIUrl":"10.1016/j.ijbiomac.2025.143639","url":null,"abstract":"<div><div>To satisfy the urgent demand to green, renewable and high value-added building and decorative materials, the research field on photoluminescent transparent wood has been developed more and more rapidly. Herein, approximately 84.7 % of lignin in balsa wood was modified to remove the chromogenic groups, and approximately 15.3 % of lignin was removed, thereby exposing more hydroxyl groups and pores of wood. Such pretreated balsa wood was then cross-linked with polyvinyl alcohol to create transparent wood films (TWF). By impregnating the treated wood with polyvinyl alcohol and blue or yellow carbon dots (BCDs or YCDs), blue and yellow photoluminescent TWFs (B-TWF and Y-TWF) were produced. The B-TWF incorporates BCDs throughout the wood following the polyvinyl alcohol (PVA), while Y-TWF has YCDs cross-linked with lignocellulose in the wood cell walls initially and then PVA in wood cell cavities. Both B-TWF and Y-TWF exhibited excellent light scattering and flexibility. Y-TWF had higher UV-blocking rate (92.4 %) and tensile strength (39.5 MPa) but lower visible light transmittance (64.8 %) compared to B-TWF (82.5 %, 22.7 MPa and 65.8 %), as well as good expansibility and water stability, which used to overlay a glass window indoors append additional resistance to UV and light scattering.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143639"},"PeriodicalIF":7.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel fluorescence and smartphone-assisted colorimetric sensing platform based on ionic liquid carbon dots for rapid detection of amylase in urine","authors":"Xiujuan Wang,&nbsp;Jiarui Wang,&nbsp;Haojia Tian,&nbsp;Chenzhao Wang,&nbsp;Xiaowen Qiu,&nbsp;Zhicong Li,&nbsp;Zhibing Wang","doi":"10.1016/j.ijbiomac.2025.143654","DOIUrl":"10.1016/j.ijbiomac.2025.143654","url":null,"abstract":"<div><div>Changes in amylase (AMY) concentration in urine serve a vital function in monitoring human health. However, the development of non-destructive, sensitive, and real-time assays for AMY concentration still faces serious challenges. In this study, a novel fluorescence/smartphone imaging colorimetric dual-mode sensing platform based on ionic liquid carbon dots (IL-CDs) was designed for the first time for assessing AMY concentration in urine. The obtained IL-CDs exhibited high sensitivity, good selectivity, and excellent stability. AMY can significantly decrease the fluorescence intensity through static quenching, enabling direct AMY concentration monitoring. Significantly, the proposed method was extended to the measurement of AMY in the urine samples with good spiked recoveries (96.98–103.81 %) as well as high accuracy. Furthermore, a smartphone-dependent detection method was developed after exploring the association between IL-CDs (G/(R + B)) fluorescence intensity at different concentrations of AMY, which simplified the AMY detection process. Through the combination of fluorescence and smartphone imaging colorimetry, it not only eliminated the damage to the human body during the detection process, but also realized the direct detection of AMY. The present method provided a fast and reliable new idea for monitoring human health.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143654"},"PeriodicalIF":7.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catalytic depolymerization of lignin by N/S modified bagasse-based hierarchical porous carbon","authors":"Hongxi Zhang,&nbsp;Xianbin Huang,&nbsp;Donglin Huang,&nbsp;Zhongke Li,&nbsp;Jun Huang,&nbsp;Xiande Yang,&nbsp;Liang Wei,&nbsp;Jing Yang","doi":"10.1016/j.ijbiomac.2025.143640","DOIUrl":"10.1016/j.ijbiomac.2025.143640","url":null,"abstract":"<div><div>Depolymerization of lignin, a complex aromatic biopolymer, presents a significant challenge in biomass conversion. While metal-based catalysts have been extensively investigated for lignin oxidative degradation, hierarchical porous carbon catalysts remain relatively underexplored. In this study, we introduce a novel approach employing nitrogen and sulfur co-doped hierarchical porous carbon (NSHPC), synthesized from sugarcane bagasse, calcium chloride (CaCl₂), and thiourea at a mass ratio of 1:2:2. The mixture underwent thermal treatment at 85 °C for 12 h, followed by drying and calcination under nitrogen atmosphere at 800 °C for 2 h. Subsequently, impurities were removed via dilute hydrochloric acid treatment. The resultant NSHPC catalyst effectively depolymerized lignin, specifically cleaving C_α-C_β and C_β-O bonds, at 140 °C for 4 h in methanol under 1 MPa oxygen pressure, achieving a high conversion rate of 98.7 % for lignin model compounds. The yields of key products were as follows: 67.8 % for phenol, 25.9 % for benzoic acid, and 33.2 % for methyl benzoate. Mechanistic investigations revealed that NSHPC facilitates the generation of superoxide anions from oxygen and active hydrogen species from methanol, which are crucial for bond depolymerization. Furthermore, experimental validation using authentic lignin samples corroborated the potential of NSHPC as an efficient catalyst for lignin depolymerization.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143640"},"PeriodicalIF":7.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium alginate hydrogel platelet rich plasma exosome loaded for synergistic hemostasis","authors":"Di Sun ,&nbsp;Haichao Yu ,&nbsp;Hechen Shen ,&nbsp;Yiran Wang ,&nbsp;Tianqi Su ,&nbsp;Hongbo Guo ,&nbsp;Xiong Xing ,&nbsp;Weikang Wang ,&nbsp;Li Wei ,&nbsp;Wenqing Gao ,&nbsp;Yi Yao ,&nbsp;Bingyu Xiu ,&nbsp;Jianing Wang ,&nbsp;Xuesong Zhang ,&nbsp;Yanchuan Guo","doi":"10.1016/j.ijbiomac.2025.143447","DOIUrl":"10.1016/j.ijbiomac.2025.143447","url":null,"abstract":"<div><div>Uncontrollable traumatic bleeding caused by surgery or traffic accidents is an important factor leading to high mortality rates. Therefore, there is an urgent hemostatic materials with fast, effective, and safe to achieve rapid hemostasis. Traditional hemostatic materials have limited effect in deep irregular tissues or massive bleeding scenes, while exocrine hydrogel can break through limitations by quickly stopping bleeding and reducing secondary injury. The gelatin time of an injectable 10 % oxidized sodium alginate-10 % gelatin (OSA-G) hydrogel was 8 s. Platelet-rich plasma exosomes (PRP-Exo) from the autologous biological macromolecule play a crucial role in blood coagulation and hemostasis. The OSA-G hydrogel loaded with PRP-Exo exhibited superior effects on proliferation and angiogenesis in vitro. The APTT and thromboelastography of the OSA-G-EXO group were significantly reduced, indicating the occurrence of intrinsic coagulation pathways and the blood was in a hypercoagulable state and promoting hemostasis. Meanwhile, it has been confirmed in the rat tail-cutting model and the liver bleeding model that the hemostasis time is approximately 30 s, promoting skin healing in the skin wound model. By providing a controlled environment for coagulopathy or anticoagulant-treated patients, it shows a good clinical application prospect.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143447"},"PeriodicalIF":7.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143885870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short neuropeptide F receptors regulate feeding and reproduction in Hyphantria cunea","authors":"Lili Sun ,&nbsp;Chunmiao Sun ,&nbsp;Jingjing Yin ,&nbsp;Timothy W. Moural ,&nbsp;Fang Zhu ,&nbsp;Chuanwang Cao","doi":"10.1016/j.ijbiomac.2025.143604","DOIUrl":"10.1016/j.ijbiomac.2025.143604","url":null,"abstract":"<div><div>Neuropeptides are endogenous active compounds in the nervous system involved in regulating physiological processes such as growth, development, reproduction, and dynamic balance. In this study, two short neuropeptide F receptors (sNPFRs) were identified in <em>Hyphantria cunea</em> and exhibit different expression patterns among various developmental stages and tissues. The expression levels of s<em>NPFR-A10</em> and s<em>NPFR-A11</em> reach peak during the 6th and 1st instar larvae stages, respectively. Both genes are highly expressed in the midgut, Malpighian tubules, and testis. Silencing gene expressions by using small interference RNAs in fifth instar larvae led to lower food intake, decreased growth rate, and reduced glucose and triglyceride content compared to the controls. Furthermore, silencing of <em>sNPFRs</em> in the pupal stage decreased egg production and hatchability, altered the morphology of eukaryotic sperm tracts, and impaired fertility by disrupting eupyrene sperm formation and motility. Molecular docking showed that <em>sNPFRs</em> were activated by the mature peptide sNPF using AlphaFold analysis. These findings demonstrate that sNPFRs are involved in the regulation of reproductive processes and are part of a larger neuropeptide network that integrates feeding, growth and development, and reproductive physiology in <em>H. cunea</em>, highlighting their potential as targets for control strategies.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143604"},"PeriodicalIF":7.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan/collagen films loaded with Pleurotus ostreatus polysaccharide-epigallocatechin gallate conjugates with excellent antioxidant and antibacterial activities for pork preservation","authors":"Xukai Niu ,&nbsp;Mengyan Xu ,&nbsp;Xiaoyue Han ,&nbsp;Wenhui Liang ,&nbsp;Qianxi Han ,&nbsp;Bo Liu ,&nbsp;Yanfen Cheng ,&nbsp;Shaojun Yun ,&nbsp;Feier Cheng ,&nbsp;Cuiping Feng ,&nbsp;Jinling Cao","doi":"10.1016/j.ijbiomac.2025.143617","DOIUrl":"10.1016/j.ijbiomac.2025.143617","url":null,"abstract":"<div><div>The purpose of this study was to develop a multifunctional active food packaging composite films to solve the environmental and health problems of traditional petroleum-based plastic packaging and for food preservation. The chitosan (CS) based-functional films were prepared by blending collagen (Clg), <em>Pleurotus ostreatus</em> polysaccharide-Epigallocatechin gallate conjugates (POP-EGCG) and ε-Polylysine (ε-PL, PL). The mechanical, physical and chemical properties, antioxidant, antibacterial activity and application in pork preservation were studied. The results showed strong intermolecular hydrogen bond interactions among Clg, POP-EGCG, and ε-PL led to denser, more uniform film surfaces and cross-sections, which significantly enhanced the thermal stability, crystallinity, tensile strength (221.73 %), and elongation at break (21.33 %) of the CS/Clg/POP-EGCG/PL film (<em>P</em> &lt; 0.05). The CS/Clg/POP-EGCG/PL films exhibited excellent water resistance, oxygen barrier properties, UV blocking, slow-release capability, and biosafety. They also demonstrated strong antioxidant (DPPH: 93.10 ± 0.51 %; ABTS: 94.13 ± 0.57 %) and antibacterial effects, thus effectively delaying pork lipid oxidation and microbial growth during refrigeration and extending shelf life by 5 days. This study offers a novel active packaging strategy to extend fresh pork shelf life, facilitating practical applications of CS/CO/POP-EGCG/PL films in food packaging.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143617"},"PeriodicalIF":7.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An innovative “double-locked” CRISPR/Cas12a system based on DNAzyme for the precise imaging of microRNAs in living cells","authors":"Lu Lu,&nbsp;Shihua Gan,&nbsp;Shixiu Xiao,&nbsp;Xiaomei Mu,&nbsp;Shulin Zhao,&nbsp;Jianniao Tian","doi":"10.1016/j.ijbiomac.2025.143605","DOIUrl":"10.1016/j.ijbiomac.2025.143605","url":null,"abstract":"<div><div>We constructed a “double-locked” CRISPR/Cas12a system based on DNAzyme for miRNA detection and precise imaging. One lock function to close the catalytic activity of DNAzyme, while the other lock serves to inhibit the cleavage function of CRISPR-induced RNA (crRNA). This “double-lock” mechanism ensures that the system is inhibited in the absence of the target molecule miRNA-141, effectively reducing the background signal. When the target miRNA-141 is present, the “lock” of DNAzyme is opened, and DNAzyme further opens the “lock” of crRNA, which activates the trans-cleavage ability of Cas12a on F-Q probe, and the fluorescence signal is restored. The linear range of miRNA-141 was 50 pmol/L ~ 15 nmol/L, and the detection limit was 47 pmol/L (S/N = 3). The system has been successfully applied to detect miRNA-141 expression levels in cell lysates. Meanwhile, this method can be applied for intracellular miRNA-141 fluorescence imaging and fluctuations in intracellular miRNA-141 expression. Overall, this strategy not only offers new prospects for programmable Cas12a detection systems, but also provides new insights for early diagnosis and screening of diseases by combining this in vitro assay with live cell imaging analysis for the detection of cancer markers.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143605"},"PeriodicalIF":7.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-anchored tissue inhibitor of matrix metalloproteinase (TIMP)-1 promotes cell death in head and neck cancer by inducing DNA damage, accumulating collagen II and disrupting cell survival mechanisms","authors":"Pengyuan Zhang ,&nbsp;Yiming Gao ,&nbsp;Renrong Tao ,&nbsp;Jipeng Zheng ,&nbsp;Yunting Fu ,&nbsp;Yidan He ,&nbsp;Qingzhe Meng ,&nbsp;Meng Huee Lee","doi":"10.1016/j.ijbiomac.2025.143558","DOIUrl":"10.1016/j.ijbiomac.2025.143558","url":null,"abstract":"<div><div>Head and neck squamous cell carcinoma (HNSCC) remains a significant global health challenge with limited therapeutic options. Here, we investigate a novel membrane-anchored variant of Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1), designated T1^Pr, as a potential therapeutic agent against HNSCC. Utilizing a comprehensive approach involving biochemical, cellular, animal, mRNA-sequencing, and omics techniques, we demonstrate that T1^Pr exhibits remarkable anti-tumorigenic effects in CAL27 cells in vitro and in vivo. Phospho-proteomics analysis revealed that T1^Pr induces profound changes in the cellular phosphorylation landscape, particularly targeting critical cell cycle regulators like CDK1 and CSNK2A1. Further, T1^Pr disrupts nuclear transport and cell cycle pathways, causes chromosomal DNA damage possibly by impeding the nuclear localization of membrane-associated MMPs and ADAM proteinases. Notably, T1^Pr triggers significant extracellular matrix remodeling, including collagen II accumulation, and suppresses numerous pro-tumor genes and proteins. T1^Pr also upregulates tumor-suppressive genes like PAWR, TPM1, and THBS1 linked to cytoskeletal stability and apoptosis induction. Functionally, T1^Pr drives CAL27 cells toward apoptosis through persistent DNA damage and disrupted mitotic checkpoints. Importantly, T1^Pr does not compromise immune checkpoint mechanisms. These findings position T1^Pr as a promising molecular therapeutic approach for targeting invasive cancers, particularly HNSCC, by orchestrating a comprehensive cellular shutdown through multiple interconnected mechanisms.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143558"},"PeriodicalIF":7.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review and in silico analysis of the role of survivin (BIRC5) in hepatocellular carcinoma hallmarks: A step toward precision","authors":"Nermin M. Mohamed ,&nbsp;Rania Hassan Mohamed ,&nbsp;John F. Kennedy ,&nbsp;Mahmoud M. Elhefnawi ,&nbsp;Nadia M. Hamdy","doi":"10.1016/j.ijbiomac.2025.143616","DOIUrl":"10.1016/j.ijbiomac.2025.143616","url":null,"abstract":"<div><div>Hepatocellular carcinoma (HCC) is a complex malignancy driven by the dysregulation of multiple cellular pathways. Survivin, a key member of the inhibitor of apoptosis (IAP) family, plays a central role in HCC tumorigenesis and progression. Despite significant research, a comprehensive understanding of the contributions of survivin to the hallmarks of cancer, its molecular network, and its potential as a therapeutic target remains incomplete. In this review, we integrated bioinformatics analysis with an extensive literature review to provide deeper insights into the role of survivin in HCC. Using bioinformatics tools such as the Human Protein Atlas, GEPIA, STRING, TIMER, and Metascape, we analyzed survivin expression and its functional associations and identified the top 20 coexpressed genes in HCC. These include TK1, SPC25, SGO2, PTTG1, PRR11, PLK1, NCAPH, KPNA2, KIF2C, KIF11, HJURP, GTSE1, FOXM1, CEP55, CENPA, CDCA3, CDC45, CCNB2, CCNB1 and CTD-2510F5.4. Our findings also revealed significant protein–protein interactions among these genes, which were enriched in pathways associated with the FOXM1 oncogenic signaling cascade, and biological processes such as cell cycle regulation, mitotic checkpoints, and diseases such as liver neoplasms.</div><div>We also discussed the involvement of survivin in key oncogenic pathways, including the PI3K/AKT, WNT/β-catenin, Hippo, and JAK/STAT3 pathways, and its role in modulating cell cycle checkpoints, apoptosis, and autophagy. Furthermore, we explored its interactions with the tumor microenvironment, particularly its impact on immune modulation through myeloid-derived suppressor cells (MDSCs), tumor-associated macrophages, and natural killer cell function in HCC. Additionally, we highlighted its involvement in alkylglycerone phosphate synthase (AGPS)-mediated lipid reprogramming and identified important gaps in the survivin network that warrant further investigation. This review also examined the role of survivin in cancer stemness, inflammation, and virally mediated hepatocarcinogenesis. We evaluated its potential as a diagnostic, prognostic, predictive, and pharmacodynamic biomarker in HCC, emphasizing its relevance in precision medicine. Finally, we summarized emerging survivin-targeted therapeutics and ongoing clinical trials, underscoring the need for novel strategies to effectively target survivin in HCC.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143616"},"PeriodicalIF":7.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced antibacterial and wound healing efficacy of a novel CQDs@AgNPs@CS-based nanocomposites: A multifunctional approach for advanced wound care","authors":"Sanae El Ghacham ,&nbsp;Lamia Hejji ,&nbsp;Youssef Aoulad El Hadj Ali ,&nbsp;Anass Wahby ,&nbsp;Lahcen Tamegart ,&nbsp;Luis Pérez-Villarejo ,&nbsp;Zakaria Mennane ,&nbsp;Badredine Souhail ,&nbsp;Abdelmonaim Azzouz","doi":"10.1016/j.ijbiomac.2025.143621","DOIUrl":"10.1016/j.ijbiomac.2025.143621","url":null,"abstract":"<div><div>Wound healing, particularly in the presence of bacterial infections, remains a significant challenge in clinical settings. This study explores the synthesis and characterization of carbon quantum dots (CQDs), silver nanoparticles (AgNPs), and chitosan (CS), designated as CQDs@AgNPs@CS nanocomposites. The primary aim is to evaluate its wound healing efficacy and antibacterial activity. An <em>in vivo</em> incisional wound model in mice is utilized alongside <em>in vitro</em> assessments against clinically relevant Gram-positive (<em>Staphylococcus aureus</em>) and Gram-negative (<em>Escherichia coli</em>, <em>Acinetobacter baumannii</em>, and <em>Klebsiella pneumoniae</em>) bacteria strains. The nanocomposites are characterized using X-ray diffraction, Fourier-transform infrared spectroscopy, zeta potential analysis, and Scanning electron microscopy to confirm its structural integrity and functional properties. Preliminary results indicate that the CQDs@AgNPs@CS nanocomposites significantly enhance wound healing and demonstrate potent antibacterial activity, with minimum bactericidal concentrations and minimum inhibitory concentrations ranging from 0.234 to 7.5 mg/mL. Histological analysis indicates that wounds treated with the CQDs@AgNPs@CS nanocomposites exhibit accelerated re-epithelialization, improved wound repair, and a reduced inflammatory response, as evidenced by decreased neutrophil counts in the skin tissue, compared to untreated wounds and those treated with individual components (CQDs, AgNPs, CS) as well as the CQDs@CS composite. Notably, the CQDs@AgNPs@CS nanocomposites also display impressive antioxidant activity, achieving an 80.21 % radical scavenging capacity against DPPH^•. These findings suggest that CQDs@AgNPs@CS nanocomposites hold great promise for advanced wound care strategies, effectively integrating healing efficacy and infection control into a single formulation.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143621"},"PeriodicalIF":7.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}