International Journal of Biological Macromolecules最新文献

{"title":"Fabrication of alginate dialdehyde-gelatin crosslinked hydrogels incorporated with cinnamaldehyde nanoparticles for meat preservation.","authors":"Ce Shi, Rui Xu, Wei Hu, Tariq Aziz, Majid Alhomrani, Haiying Cui, Lin Lin","doi":"10.1016/j.ijbiomac.2025.140063","DOIUrl":"10.1016/j.ijbiomac.2025.140063","url":null,"abstract":"<p><p>Meat spoilage caused by Salmonella Typhimurium (S. typhimurium) contamination has become a major concern worldwide. Herein, the antibacterial mechanisms of cinnamaldehyde (CIN) against S. typhimurium were evaluated, and the alginate dialdehyde (ADA)-gelatin (Gel) crosslinked hydrogels loaded with CIN nanoparticles prepared by zein and carboxymethyl chitosan (ZCC/CINNPs) were fabricated for meat preservation. Results showed the efficient antibacterial effects of CIN via interfering with the S. typhimurium cell membrane, increasing the membrane permeability, disrupting the membrane integrity, thereby leading to the intracellular materials leakage and cell death. ZCC/CINNPs were prepared and characterized, showing potent antibacterial and antioxidant activities, and excellent stabilities. ZCC/CINNPs addition into ADA/Gel hydrogels markedly improved the barrier, antibacterial and antioxidant properties, while decreased the water loss. Furthermore, the release behaviors of CIN from the composite hydrogels in different food systems suggested the suitability for the preservation of various food products. Practical application results showed the composite hydrogels with 25 % ZCC/CINNPs (AGH-ZC 25 %) enhanced the storage stability of chicken and pork meat at 4 °C, which decreased the weight loss rate, maintained the pH, color, and textural stabilities, retarded the microbial growth and lipid oxidation of meat samples. Findings here suggested AGH-ZC 25 % hydrogels had great potential in meat preservation.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140063"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Exosome-loaded microneedle patches: Promising factor delivery route\" [Int. J. Biol. Macromol. 243 (2023) 125232].","authors":"Sonia Fathi-Karkan, Morteza Heidarzadeh, Maryam Taghavi Narmi, Narges Mardi, Hassan Amini, Sepideh Saghati, Fateme Nazary Abrbekoh, Solmaz Saghebasl, Reza Rahbarghazi, Ali Baradar Khoshfetrat","doi":"10.1016/j.ijbiomac.2025.139995","DOIUrl":"10.1016/j.ijbiomac.2025.139995","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"139995"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Facile degradation of chitosan-sodium alginate-chromium (III) gel in relation to leather re-tanning and filling\" [International Journal of Biological Macromolecules, 240 (2023) 124437].","authors":"Xiaoyu Guan, Bingyuan Zhang, Shiyong Liu, Meng An, Qingxin Han, Dongping Li, Ping Rao","doi":"10.1016/j.ijbiomac.2025.139961","DOIUrl":"10.1016/j.ijbiomac.2025.139961","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"139961"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Bovine serum albumin-liposome stabilized high oil-phase emulsion: Effect of liposome ratio on interface properties and stability\" [Int. J. Biol. Macromol. 266 (2024) 131040].","authors":"Yang Chen, Xiangzhou Yi, Zhisheng Pei, Xuan Zhang, Xia Gao, Weimin Zhang, Xuanri Shen","doi":"10.1016/j.ijbiomac.2025.139781","DOIUrl":"10.1016/j.ijbiomac.2025.139781","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"139781"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photothermal antimicrobial guar gum hydrogel cross-linked by bioactive small molecule lysine for infected wound healing.","authors":"Chufan Yang, Yan Wang, Zhiyuan Zhang, Chen Wang, Fangzheng Yu, Zheng Zhao, Zhenyu Wang, Lingling Zhang","doi":"10.1016/j.ijbiomac.2025.140328","DOIUrl":"10.1016/j.ijbiomac.2025.140328","url":null,"abstract":"<p><p>The healing of bacteria-infected wounds has long posed a significant clinical challenge. Traditional hydrogel wound dressings often lack self-healing properties and effective antibacterial characteristics, making wound healing difficult. In this study, a bioactive small molecule cross-linking agent 4-FPBA/Lys/4-FPBA (FLF) composed of 4-formylphenylboronic acid (4-FPBA) and lysine (Lys) was utilized to cross-link guar gum (GG) and a tannic acid/iron (TA/Fe³⁺) chelate through multiple dynamic bonds, leading to the formation of a novel self-healing hydrogel dressing GG-FLF/TA/Fe. The hydrogel exhibited excellent stretchability and self-healing ability, which enabled it to adapt to irregular wound sites. Meanwhile, the hydrogel demonstrated remarkable antibacterial efficacy (>99 %) facilitated by the synergistic effects of photothermal properties and aromatic Schiff bases. Additionally, it had adjustable rheological properties, good mechanical characteristics, conductivity, antioxidant characteristics and biocompatibility. Notably, the GG-FLF/TA/Fe hydrogel dressing irradiated with NIR displayed superior therapeutic effects in a mouse wound infection model (wound healing rate: 94.8 %), promoting recovery from bacterially infected wounds by enhancing collagen deposition, facilitating the formation of skin appendages and blood vessels, and regulating inflammatory factors. In summary, this study presented a novel approach to prepare biologically active antibacterial polysaccharide hydrogels and highlighted the substantial potential of this hydrogel as a biomedical antibacterial dressing.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140328"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Augmented silver sulfadiazine nanostructured lipid carriers impregnated collagen sponge for promoting burn wound healing.","authors":"Hadel A Abo El-Enin, Isra H Ali, Ibrahim A Naguib, Nahla Sameh Tolba, Hend Mohamed Abdel-Bar","doi":"10.1016/j.ijbiomac.2025.140371","DOIUrl":"10.1016/j.ijbiomac.2025.140371","url":null,"abstract":"<p><p>Silver sulfadiazine (SSD) is a widely used antibacterial agent for burn wound treatment owing to its capability in re-epithelialization and wound healing. However, due to its low solubility, the need for an effective drug delivery system is mandatory. This study aimed to optimize SSD nanostructured lipid-based carriers (NLCs), incorporated in a collagen sponge form as an innovative topical dosage form for effective burn wound treatment. SSD-NLCs were prepared by applying Box-Behnken design and characterized in terms of particle size, zeta potential, and entrapment efficiency (EE). The optimized SSD-NLCs formula was selected and incorporated into a cross-linked collagen sponge and lyophilized for 24 h. The SSD-NLCs sponge morphological structure, porosity, swelling ratio, in vitro drug release profile and antibacterial activity were assessed. Furthermore, investigating the competitive inhibitory efficiency of SSD against para-aminobenzoic acid (PABA), the native ligand for the dihydropteroate synthetase enzyme based on the calculated binding free energy using the CB-Dock docking server was evaluated. Additionally, the wound healing activity and histopathological studies were evaluated on a second-degree burn wounds in a rat model. The optimized SSD-NLCs were spherical, possessing size of 115.69 ± 3.25 nm, EE% of 89.69 ± 1.36 % and a porosity of 71.22 %. Furthermore, the SSD-NLCs sponge showed an enhanced swelling ratio and improved antimicrobial activity compared to SSD. Finally, in vivo studies in rats showed that SSD-NLCs sponge are effective wound healing formulation owing to their ability to improve the quality of tissue regeneration without scars formation. Results showed that SSD-NLCs sponge can enhance the SSD efficacy in treatment of burn wounds. Further toxicological studies are still needed before clinical application.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140371"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurately sensing analysis of active adenine DNA glycosylase (MutY) via the high identification/excision capability to specific base-mismatches of dsDNA chains","authors":"Jingqi Sun ,&nbsp;Jiaxin Li ,&nbsp;Yanzhi Xing ,&nbsp;Han Leng ,&nbsp;Wei Chen ,&nbsp;Yanfeng Zhang ,&nbsp;Xuwei Chen","doi":"10.1016/j.ijbiomac.2025.141789","DOIUrl":"10.1016/j.ijbiomac.2025.141789","url":null,"abstract":"<div><div>Adenine DNA glycosylase (MutY) is a crucial member of DNA glycosylase family, and the abnormal expression of the human MutY homologs is associated with the pathogenesis of various diseases, therefore, convenient and cost-effective assessing the activity of MutY holds significant biological and medical importance. Herein, the precise identification/excision capacity of MutY to mismatched G-A base pair of dsDNA chains and the DNA-template-dependant fluorescence behaviors of copper nano cluster (CuNCs) was exploited for the accurate sensing of active MutY. Hairpin DNA with G-A base mismatch was excised by MutY to produce dsDNA chains with repetitive AAT-TTA base pairs. The newly formed dsDNA provided more active sites for the growth of CuNCs compared to the original hairpin DNA, resulting in the significantly enhanced fluorescence of final CuNCs. MutY was accurately quantified with a detection limit of 9.98 nmol L⁻¹. The developed sensing protocol exhibited excellent selectivity toward MutY over various ions, neutral biomolecules, and protein species. Most importantly, The sensing system is capable to distinguish the active MutY from other MutY homologs with low activity, e.g., de-[4Fe4S] cluster MutY (DIS-MutY), and the practicality was well demonstrated by detecting active MutY contents in various cell lysates.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"306 ","pages":"Article 141789"},"PeriodicalIF":7.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium alginate aerogel derived SiC@Co-C 3D network enhances electromagnetic wave absorption and thermal conductivity of PDMS based composite","authors":"Lilin Xiang ,&nbsp;Duo Pan ,&nbsp;Junting Lei ,&nbsp;Najla AlMasoud ,&nbsp;Taghrid S. Alomar ,&nbsp;Issayev Gani Issaulu ,&nbsp;Yaming Wang ,&nbsp;Zeinhom M. El-Bahy ,&nbsp;Chuntai Liu ,&nbsp;Zhanhu Guo ,&nbsp;Seilkhan Ainur ,&nbsp;Zhexenbek Toktarbay","doi":"10.1016/j.ijbiomac.2025.141539","DOIUrl":"10.1016/j.ijbiomac.2025.141539","url":null,"abstract":"<div><div>Interface packaging materials with both electromagnetic wave absorption and high thermal conductivity remains a research hotspot in modern electronics industry and aerospace field. In this paper, the cobalt alginate aerogel derived from biomass sodium alginate was carbothermal reduced to obtain a three-dimensional skeleton structure composed of zero-dimensional Co metal particles, one-dimensional silicon carbide (SiC) whiskers and biomass derived carbon (C). The dielectric-electromagnetic effect of the filler network makes the obtained polydimethylsiloxane (PDMS) based composite (PDMS/SiC@Co-C) exhibits good electromagnetic wave absorption performance, with the lowest reflection loss (RL_min) value can reach −40.08 dB, and the effective absorption bandwidth (EAB_max) can reach 4.16 GHz at the thickness of 1.9 mm. The unique three-dimensional overlapping structure makes the thermal conductivity of PDMS/SiC@Co-C increase to 1.856 W/m·K, which is 1345 % higher than that of PDMS. This work provides an important reference value for the development of interface packaging materials used in microelectronics and aerospace fields.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"306 ","pages":"Article 141539"},"PeriodicalIF":7.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the substrate-binding pocket: Endowing carbonyl reductase BaSDRX with intriguing inconsistent stereoselectivity towards similar structured β-Ketoesters","authors":"Aipeng Li ,&nbsp;Shuqi Guo ,&nbsp;Xinying Wang ,&nbsp;Tianyi Lu ,&nbsp;Yifan Wang ,&nbsp;Lianbing Zhang ,&nbsp;Qiang Fei","doi":"10.1016/j.ijbiomac.2025.141591","DOIUrl":"10.1016/j.ijbiomac.2025.141591","url":null,"abstract":"<div><div>Tailoring of carbonyl reductase with desired stereoselectivity for the asymmetric reduction of given <em>β</em>-ketoesters continues to pose a significant challenge. The reconstruction of substrate-binding pocket endowed carbonyl reductase BaSDRX with inconsistent stereoselectivity towards similar structured <em>β</em>-ketoesters. The variant F86C/E142A showed reversed stereoselectivity towards <em>β</em>-ketoesters with a larger substituent on the carbonyl side, giving antiPrelog alcohols with ee values varied from 52.8 % to 95.8 %. However, it displayed only a decreased stereoselectivity for <em>β</em>-ketoesters that contain a smaller methyl group at the same position. Analysis of enzyme-substrate complexes showed that a newly formed groove provided more spaces and chances for <em>β</em>-ketoester with a larger substituent on the carbonyl side to approach the catalytic triad of F86C/E142A in antiPrelog-preferred binding modes. For the <em>β</em>-ketoesters with a smaller methyl group on the carbonyl side, the introduction of mutations exerted less effect on the proximity of the substrate with antiPrelog-preferred binding modes to the catalytic residues. Meanwhile, both <em>β</em>-ketoesters with smaller group and larger group on carbonyl side underwent minor alterations in Prelog-preferred conformation. All of these made the variant F86C/E142A exhibited inconsistent stereoselectivity towards <em>β</em>-ketoesters possessing analogous structure. These results offered detailed mechanisms that govern the stereoselectivity in the enzyme-mediated asymmetric reduction of <em>β</em>-ketoesters.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"306 ","pages":"Article 141591"},"PeriodicalIF":7.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH-responsive nanoparticles for oral delivery of RNAi for sustained protection against Spodoptera exigua","authors":"Yiping Jiang ,&nbsp;Suman Zong ,&nbsp;Xiaofeng Wang ,&nbsp;Keyan Zhu-Salzman ,&nbsp;Jing Zhao ,&nbsp;Liubin Xiao ,&nbsp;Dejin Xu ,&nbsp;Guangchun Xu ,&nbsp;Yongan Tan","doi":"10.1016/j.ijbiomac.2025.141763","DOIUrl":"10.1016/j.ijbiomac.2025.141763","url":null,"abstract":"<div><div>To enhance the RNAi efficiency of dsRNA against the <em>Spodoptera exigua</em> through a feeding method, we developed a pH-responsive nanoparticle, chitosan-polyethylene glycol-carboxyl (CS-PEG-COOH). This nanoparticle enhances RNAi efficiency by improving dsRNA stability in the midgut of <em>S. exigua</em> and can intelligently release dsRNA under alkaline conditions. Firstly, the CS-PEG-COOH carrier was prepared <em>via</em> cross-linking reactions, and the mass ratio of dsRNA to CS-PEG-COOH was obtained using electrophoretic mobility. The carrier composite materials were then characterized using isothermal titration calorimetry (ITC), transmission electron microscopy (TEM), atomic force microscopy (AFM), and Zeta potential analysis. The stability and delivery efficiency of the dsRNA/CS-PEG-COOH complex were then verified using electrophoretic mobility and fluorescence labeling methods. Finally, the RNAi efficiency and synergistic mechanism of the complex were analyzed using feeding methods and RNA-seq. The results show that CS-PEG-COOH (40.16 nm size, + 6.44 mV charge) forms a clustered complex with dsRNA through hydrogen bonding and hydrophobic interactions. CS-PEG-COOH significantly enhancing the stability and delivery efficiency of dsRNA in the midgut of <em>S. exigua</em>. Additionally, at pH &gt; 8, dsRNA could be released from dsRNA/CS-PEG-COOH. The RNAi results showed that, dsRNA/CS-PEG-COOH could effectively inhibit the expression of the Acetylcholinesterase (<em>Ace1 + Ace2</em>) gene (65 %), and led to significantly increase mortality (51.82 %), a prolonged developmental period (25 %) and reduced egg production (22.02 %). The physiological and molecular synergistic mechanisms were revealed by RNA-seq analysis. The CS-PEG-COOH-loaded ds<em>ACE1</em> + ds<em>ACE2</em> led to down-regulation of genes related to drug metabolism, hormone synthesis, and stratum corneum biosynthesis, which inhibited insect growth and development. Overall, We developed an appropriate delivery method for dsRNA application in Lepidoptera, providing a basis for developing RNA pesticides with high efficiency and environmental safety.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"306 ","pages":"Article 141763"},"PeriodicalIF":7.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}