International Journal of Biological Macromolecules最新文献_第8页

Effect of fine molecular structure of debranched starch on its digestibility 脱支淀粉精细分子结构对其消化率的影响

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143703

Lu Yang , Ruqing Sun , Zihui Xu , Yang Qin , Liu Xiong , Rui Shi , Qingjie Sun

{"title":"Effect of fine molecular structure of debranched starch on its digestibility","authors":"Lu Yang , Ruqing Sun , Zihui Xu , Yang Qin , Liu Xiong , Rui Shi , Qingjie Sun","doi":"10.1016/j.ijbiomac.2025.143703","DOIUrl":"10.1016/j.ijbiomac.2025.143703","url":null,"abstract":"<div><div>Debranched starch (DBS) has shown promise in the development of slow-digesting starchy foods due to its abundant short-chain glucans (SCGs). However, the effect of its fine molecular structure on digestibility remains unclear. In this study, DBS with varying contents of partially debranched amylopectin (PDA) and debranched SCGs were obtained by adjusting the enzyme content and hydrolysis time, followed by analysis of their multiscale structure and digestibility. The results showed that as the SCGs content increased from 54.16 % to 100 %, the recrystallization rate of DBS significantly increased from 0.19 to 0.62, accompanied by a morphological transition from a worm-like to a spherical structure. SAXS analysis confirmed that high SCGs content led to an ordered and dense DBS structure. Correlation analysis revealed stage-dependent effects of debranching degree and B2 + B3 chain ratio on DBS digestibility: B2 + B3 chains primarily enhanced resistant starch (RS) content when SCGs <85 %, whereas both factors synergistically increased both slowly digestible starch and RS content at SCGs >85 %. These findings suggest that modulating the chain length distribution of DBS is an effective strategy for tailoring its digestive characteristics.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143703"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurogranin facilitates maintaining the immunosuppressive state of hepatocellular carcinoma by promoting TGF-β1 secretion 神经颗粒蛋白通过促进TGF-β1的分泌来维持肝癌细胞的免疫抑制状态

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143716

Dongjie Ye , Zhu Zhang , Yuxin Yao , Banglun Pan , Hao Wu , Xinyu Zhang , Xiaoqian Wang , Nanhong Tang

{"title":"Neurogranin facilitates maintaining the immunosuppressive state of hepatocellular carcinoma by promoting TGF-β1 secretion","authors":"Dongjie Ye , Zhu Zhang , Yuxin Yao , Banglun Pan , Hao Wu , Xinyu Zhang , Xiaoqian Wang , Nanhong Tang","doi":"10.1016/j.ijbiomac.2025.143716","DOIUrl":"10.1016/j.ijbiomac.2025.143716","url":null,"abstract":"<div><div>Immunotherapy has revolutionized cancer treatment, but its effectiveness is limited due to the complexity of the tumor immune microenvironment. Identifying reliable biomarkers that can predict immunotherapy response is essential for enhancing treatment strategies. This study evaluated the potential of <em>Neurogranin</em> (<em>NRGN</em>) as a biomarker for prognosis and immunotherapy response across multiple cancers. Through pan-cancer bioinformatics analyses, coupled with <em>in vitro</em> and <em>in vivo</em> experiments, we explored <em>NRGN</em>'s differential expression across various cancer types and its role in the immune microenvironment. Our approach involved database mining, immune genomic feature correlation analyses, and functional validation through <em>NRGN</em> knockdown and overexpression studies. The results revealed differential <em>NRGN</em> expression across cancers, particularly hepatocellular carcinoma (HCC), where elevated levels correlated with immune evasion, poor prognosis, and upregulation of checkpoint genes like <em>TGFB1</em>. NRGN modulated T cell activity and macrophage polarization by regulating the TGF-β pathway through interaction with TCF4 and promoting its nuclear localization, driving tumor progression. Targeting TGF-β with anti-TGF-β and anti-PD-1 antibodies additively inhibited HCC in an Nrgn-dependent manner in mice. These findings indicate that NRGN may serve as a promising immunotherapeutic target, as its overexpression predicts poor prognosis and immune evasion, thereby offering insights for improving immunotherapy and developing new treatments.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143716"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rehmannia glutinosa polysaccharides alleviate cyclophosphamide-induced immunosuppression by enhancing immunity and restoring intestinal homeostasis 地黄多糖通过增强免疫和恢复肠道稳态来缓解环磷酰胺诱导的免疫抑制

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143692

Lin Yu, Xiaofeng Shi, Xuanqi Lu, Xuping Zhao, Yaming Yu, Yushan Fang, Deyun Wang

引用次数: 0

Identification of mitochondrial energy metabolism genes associated with obstructive sleep apnea syndrome: integrated bioinformatics analysis 鉴定与阻塞性睡眠呼吸暂停综合征相关的线粒体能量代谢基因：综合生物信息学分析

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143699

Ying Jiang, Junhong Chen, Xiaofang Du, Ling Xiao, Hong Jiang, Fan Wang, Bing Wang

{"title":"Identification of mitochondrial energy metabolism genes associated with obstructive sleep apnea syndrome: integrated bioinformatics analysis","authors":"Ying Jiang, Junhong Chen, Xiaofang Du, Ling Xiao, Hong Jiang, Fan Wang, Bing Wang","doi":"10.1016/j.ijbiomac.2025.143699","DOIUrl":"10.1016/j.ijbiomac.2025.143699","url":null,"abstract":"<div><div>Obstructive sleep apnea syndrome (OSAS) is a common sleep disorder, which is closely related to abnormal mitochondrial energy metabolism in recent years. By analyzing gene expression data of OSAS, we identified differentially expressed genes (DEGs) related to mitochondrial energy metabolism, and further explored the function of NDUFA10 in OSAS and its potential diagnostic value. In this paper, we download OSAS related expression data from a public database and preprocess the data set using a standardized process. Gene set enrichment analysis (GSEA) was used to assess pathways associated with mitochondrial metabolism, and diagnostic models were constructed to assess the expression of key genes. ROC curve analysis was performed for common mitochondrial energy metabolism-related differentially expressed genes (Co-MEMRDEGs) and gene interaction networks were constructed. After data standardization, significantly differentially expressed genes were identified, among which NDUFA10 was identified as one of the genes most associated with OSAS. The constructed diagnostic model showed good prediction accuracy, and ROC curve analysis further verified its clinical diagnostic potential.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143699"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of starch molecular weight and chain length distribution on the 0–3 texture levels 淀粉分子量和链长分布对0-3级织构水平的影响

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143694

Xiaodong Tian, Junrong Huang, Hongliang Li, Chong Zhang, Tao Li, Yue Pan

{"title":"The effects of starch molecular weight and chain length distribution on the 0–3 texture levels","authors":"Xiaodong Tian, Junrong Huang, Hongliang Li, Chong Zhang, Tao Li, Yue Pan","doi":"10.1016/j.ijbiomac.2025.143694","DOIUrl":"10.1016/j.ijbiomac.2025.143694","url":null,"abstract":"<div><div>The 3–0 texture levels belong to liquids according to International Dysphagia Dietary Standardization Initiative classification. The effects of starch molecular weight and chain length distribution on 3–0 texture levels were not clear. Rice starch (Level 7) was hydrolyzed to Level 3–0 by using α-amylase. When the texture fell from Level 3 to 0, the average molecular weight of rice starch was tumbled from 9.48 × 10<sup>7</sup> to 1.17 × 10<sup>4</sup> g/mol, and the Mw/Mn of starch was reduced from 22.0 to 3.3, the chain length distribution of the shortest chain with Dp (6–12) was increased from 41.1 % to 70.5 %, while the longer chain was decreased gradually. Additionally, the height and roughness of starch molecular chains exhibited significant changes, with height decreasing from 1.1 to 0.7 nm and roughness decreasing from 0.332 to 0.208 nm. The amylopectin and amylose were partially hydrolyzed at Levels 3 and 2, and completely hydrolyzed at Levels 1 and 0, converting into smaller molecular weight components. Reducing the molecular weight of rice starch through enzymatic hydrolysis could lower the texture levels from 7 (solid) to 3–0 (liquid). These findings provide a theoretical basis for starch-based liquid food with texture Levels of 3–0.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143694"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NF-kappa-B inhibitor alpha mediates cancer stemness characteristics in oral squamous cell carcinoma by interacting with cathepsin B nf - κ B抑制剂α通过与组织蛋白酶B相互作用介导口腔鳞状细胞癌的癌干性特征

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143690

Tian Xu Qin , Ying Ying Zhu , Wai Hoe Ng , Siew Kit Ng , Min Fey Chek , Kai Dun Tang

{"title":"NF-kappa-B inhibitor alpha mediates cancer stemness characteristics in oral squamous cell carcinoma by interacting with cathepsin B","authors":"Tian Xu Qin , Ying Ying Zhu , Wai Hoe Ng , Siew Kit Ng , Min Fey Chek , Kai Dun Tang","doi":"10.1016/j.ijbiomac.2025.143690","DOIUrl":"10.1016/j.ijbiomac.2025.143690","url":null,"abstract":"<div><div>The role of NF-kappa-B inhibitor alpha (IκBα), a well-established negative regulator of the NF-κB signalling pathway in cancer is complex, as evidenced by either promoting or suppressing tumourigenesis, depending on the cancer type, however, in oral squamous cell carcinoma (OSCC) remains unelucidated. Here, for the first time, we report that the elevated levels of both NKFBIA mRNA and IκBα protein in OSCC tumour tissues and OSCC cell lines. Meanwhile, IκBα silencing resulted in the suppression of cell proliferation, migration and invasion in OSCC. In addition, we demonstrated that IκBα can mediate the OSCC stemness and epithelial-mesenchymal transition (EMT) characteristics by directly interacting with cathepsin B (CTSB) and thus, facilitating the progression toward carcinogenesis. More importantly, we identified Psammaplin A (PsA), a natural metabolite derived from marine sponges that would disrupt the IκBα-CTSB interaction <em>via</em> competition, thereby reducing oral spheres formation, cell viability and OSCC tumour growth <em>in vivo</em>, clearly demonstrating its potential as an effective therapeutic agent that specifically targets this oncogenic complex. In summary, we have unveiled a novel mechanism underlying the oncogenic role of IκBα-CTSB complex in OSCC, which may offer the therapeutic potential of targeting this complex with PsA for the treatment of OSCC.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143690"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oat beta-glucan inhibited obesity in mice though stem cell remodeling 燕麦β -葡聚糖通过干细胞重塑抑制小鼠肥胖

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143747

Tengteng Ji , Bing Fang , Ming Zhang , Le Cheng , Xinlei Yuan , Yutong Jin , Fang Wu , Chenyan Zheng , Yumeng Ma

{"title":"Oat beta-glucan inhibited obesity in mice though stem cell remodeling","authors":"Tengteng Ji , Bing Fang , Ming Zhang , Le Cheng , Xinlei Yuan , Yutong Jin , Fang Wu , Chenyan Zheng , Yumeng Ma","doi":"10.1016/j.ijbiomac.2025.143747","DOIUrl":"10.1016/j.ijbiomac.2025.143747","url":null,"abstract":"<div><div>Oat beta-glucan has been reported to be beneficial to glucose and lipid metabolism in human and animal studies. However, weight-loss was shown only in animal studies, whereas human studies reported an improvement in hyperlipidemia. This study investigated the effects of oat beta-glucan on obesity and related metabolic disturbances at the doses based on the Chinese recommended daily intake of 100 g of whole grains which contain approximately 10 % β-glucan. Our data showed that 200 mg/kg efficiently blocked the process of obesity as determined by significantly decreased body weight (7.57 g) and adipose tissue mass (5.24 g) after the 4-week intervention. Lower serum low-density lipoprotein cholesterol (0.32 mmol/L) and reduced atherosclerosis were also observed. Oat beta-glucan improved hepatic lipid metabolism at the low dose (100 mg/kg) and restored it to normal levels in lean mice at the high dose. To our knowledge, our study is the first to analyze the effects of oat beta-glucan on adipose stem cells and reveal the anti-obesity mechanism of oat β-glucan is modulating the adipose stem cell microenvironment. This study determined an efficient supplementary dose for blocking high-fat diet-induced obesity, which not only improved lipid metabolism but also altered stem cell composition in adipose tissue.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143747"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards a better understanding of the structure-function relationship of chestnut starches 为了更好地了解板栗淀粉的结构-功能关系

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143702

Simeng Li , Chen Chao , Jinglin Yu , Les Copeland , Yuedong Yang , Shujun Wang

{"title":"Towards a better understanding of the structure-function relationship of chestnut starches","authors":"Simeng Li , Chen Chao , Jinglin Yu , Les Copeland , Yuedong Yang , Shujun Wang","doi":"10.1016/j.ijbiomac.2025.143702","DOIUrl":"10.1016/j.ijbiomac.2025.143702","url":null,"abstract":"<div><div>Chestnut starch is of increasing interest for applications in foods but has been studied to a much lesser extent than other food starches. Hence, the structure-function relationships of starches isolated from eight chestnut cultivars were examined. The starch granules were round, oval and conical in shape, with average particle size and amylose content ranging between 7.14 and 9.39 μm and 29.9 to 34.0 %, respectively. Chain length distributions showed larger differences between the starches in the A chains than in the B<sub>1</sub> ∼ B<sub>3</sub> chains. All chestnut starches exhibited a typical B-type crystalline pattern, with the relative crystallinity ranging from 34.1 to 40.6 %. Significant differences were also observed in short-range molecular order and in functional properties. Pearson correlation analyses showed that amylose content was positively correlated with peak and breakdown viscosities and negatively correlated with the rate of <em>in vitro</em> digestion of the starches. The short A chains were negatively and the B<sub>2</sub> or B<sub>3</sub> chains positively correlated with the peak and breakdown viscosities, but the opposite correlations were noted for the rate of <em>in vitro</em> digestion of the starches. This study contributes new insights into an understanding the structure-function relationships of chestnut starches and for their applications in foods.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143702"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An effective and sustainable strategy to improve the stability and adhesion of thermoplastic starch to polyester/cotton yarns via the mixture of gellan gum 通过结冷胶混合物提高热塑性淀粉对涤纶/棉纱的稳定性和附着力的有效和可持续的策略

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143727

Yixuan He , Suhao Fan , Xinyi Han , Shengyi Yuan , Yijie Zhu , Xiang Li , Wangbing Li , Ying Li , Yingnan Ma , Kang Zhang

{"title":"An effective and sustainable strategy to improve the stability and adhesion of thermoplastic starch to polyester/cotton yarns via the mixture of gellan gum","authors":"Yixuan He , Suhao Fan , Xinyi Han , Shengyi Yuan , Yijie Zhu , Xiang Li , Wangbing Li , Ying Li , Yingnan Ma , Kang Zhang","doi":"10.1016/j.ijbiomac.2025.143727","DOIUrl":"10.1016/j.ijbiomac.2025.143727","url":null,"abstract":"<div><div>Starch sizing agent had a low mechanical strength, poor stability, and weak adhesion to polyester fibers. In this study, corn starch/gellan gum composites (CS/GG) were used for sizing polyester/cotton blended yarns (P/C 80/20), and performance of sizing agents were investigated. The results showed that CS/GG composite films exhibited stronger tensile strength, more sustained inhibition long-term retrogradation of starch, better gas barrier, higher antioxidant activity, and lower water uptake compared to thermoplastic starch films. The CS/GG film, paste, and sizing at the weight ratio of 8: 2 had the superior tensile strength, viscosity stability, and adhesion to P/C 80/20, respectively. It was due to that gellan gum could form a strong hydrogen bond interaction with the hydroxyl group on starch, thereby increasing the crystallinity of the thermoplastic starch film. The rupture work of sized P/C 80/20 by CS/GG with 9: 1 and 8: 2 were 2931.3 mJ and 3024.0 mJ, respectively. CS/GG at the weight ratio of 9: 1 was more suitable for sizing P/C 80/20 than the 8: 2 in terms of cost.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143727"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition effects of xanthohumol on α-amylase and α-glucosidase: Kinetics, multi-spectral and molecular docking 黄腐酚对α-淀粉酶和α-葡萄糖苷酶的抑制作用：动力学、多光谱和分子对接

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI: 10.1016/j.ijbiomac.2025.143676

Bin Zhang, Yuansheng Deng, Yuanrui Song, Fa Yang, Yingying He, Tao Guo

{"title":"Inhibition effects of xanthohumol on α-amylase and α-glucosidase: Kinetics, multi-spectral and molecular docking","authors":"Bin Zhang, Yuansheng Deng, Yuanrui Song, Fa Yang, Yingying He, Tao Guo","doi":"10.1016/j.ijbiomac.2025.143676","DOIUrl":"10.1016/j.ijbiomac.2025.143676","url":null,"abstract":"<div><div>α-Amylase and α-glucosidase, two therapeutic targets for diabetes management, play crucial roles in carbohydrate metabolism and glucose absorption. Xanthohumol, one chalcone derived from hops, has demonstrated potential as a natural healthy phytonutrient. But its inhibition effects against α-amylase and α-glucosidase were still unclear. This study employs a comprehensive analytical strategy, including kinetics, multi-spectral, and molecular docking methods, to dissect the inhibition effects of xanthohumol against α-amylase and α-glucosidase. Our findings indicated that xanthohumol exerted a reversible mixed-type inhibition on both enzymes, with IC<sub>50</sub> values of 71.07 ± 5.82 μM for α-amylase and 32.58 ± 3.11 μM for α-glucosidase. Multi-spectral analyses (fluorescence quenching, synchronous fluorescence, 3D fluorescence, ANS-binding fluorescence, and CD) revealed that xanthohumol binding induced conformation changes and microenvironment alterations in the enzymes, thereby inhibiting their activities. Molecular docking and dynamics studies further substantiated the interaction forces between xanthohumol and the enzymes. This research provided insights into the effects of xanthohumol as an inhibitor of α-amylase and α-glucosidase, offering valuable data to support the development of xanthohumol as a natural therapeutic for diabetes management.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143676"},"PeriodicalIF":7.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0