International Journal of Biological Macromolecules最新文献_第7页

Bionanoclusters of chitosan, indole acetic acid and cobalt chloride enhance seed germination in upland cotton by modulating glyoxylate cycle enzymes and biochemical responses 壳聚糖、吲哚乙酸和氯化钴的生物纳米团簇通过调节glyoxylate cycle酶和生化反应来促进陆地棉花种子萌发

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144735

Vikram Singh , Shiwani Mandhania , Rashi Datten , Ajay Pal , Vinod Saharan , Om Prakash Ghimire

{"title":"Bionanoclusters of chitosan, indole acetic acid and cobalt chloride enhance seed germination in upland cotton by modulating glyoxylate cycle enzymes and biochemical responses","authors":"Vikram Singh , Shiwani Mandhania , Rashi Datten , Ajay Pal , Vinod Saharan , Om Prakash Ghimire","doi":"10.1016/j.ijbiomac.2025.144735","DOIUrl":"10.1016/j.ijbiomac.2025.144735","url":null,"abstract":"<div><div>Seed priming is a quick and easy technique to boost seed germination, seedling development and plant growth. Among many exogenous agents, nanoparticles are one of the promising agents which enable the controlled delivery of multiple active agents simultaneously, improving efficiency and effectiveness. In the present investigation, bionanoclusters (BNCs) composed of cobalt (Co), indole-3-acetic acid (IAA), and chitosan were synthesized and employed as seed priming agents in cotton seeds to examine their effects on seed germination as well as associated phenotypic and biochemical changes. These BNCs demonstrated monodispersity, stability, and a slow release of Co and IAA. Seed priming with 100 mg/l BNCs significantly increased the germination by 16.33 % (<em>p</em> < 0.01). Compared to the control, seedlings from BNCs-primed seeds exhibited significantly longer roots (54.27 %) and greater biomass (28.96 %) by the 12th day of germination (<em>p</em> < 0.01). Additionally, BNCs priming increased the activities of key enzymes of the glyoxylate cycle, including isocitrate lyase (20.43 %) and malate synthase (29.17 %), as well as total soluble sugar content (42.27 %) on the 3rd day of germination to support seed germination. Results highlight the promising role of BNCs in improving seed germination to improve crop productivity.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144735"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutral polysaccharide from Dendrobium officinale alleviates acute alcohol-induced liver injury via the gut microbiota-short chain fatty acids-liver axis. 铁皮石斛中性多糖通过肠道菌群-短链脂肪酸-肝轴减轻急性酒精性肝损伤。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144719

Mingxing Tao, Yujun Xie, Xinxin Fan, Xiaodong Yan, Wanyu Fan, Ruiqin Cao, Manman Li, Renshi Li, Lei Wang

{"title":"Neutral polysaccharide from Dendrobium officinale alleviates acute alcohol-induced liver injury via the gut microbiota-short chain fatty acids-liver axis.","authors":"Mingxing Tao, Yujun Xie, Xinxin Fan, Xiaodong Yan, Wanyu Fan, Ruiqin Cao, Manman Li, Renshi Li, Lei Wang","doi":"10.1016/j.ijbiomac.2025.144719","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.144719","url":null,"abstract":"<p><p>The structure and bioactivities of a new neutral polysaccharide (DOP) from Dendrobium officinale were investigated. The DOP was obtained by hot-water extraction, ethanol precipitation and further purification with DEAE cellulose-52. The DOP molecular weight of the main peak was 422.74 kDa and mainly contained glucose and mannose with molar ratios of about 1:3. In acute alcoholic liver injury (AALI) model, DOP attenuated liver injury by reducing ALT, AST and TG levels, alleviated oxidative stress by restoring SOD and CAT activities and reducing MDA content, and suppressed inflammation by inhibiting lipopolysaccharide (LPS), IL-6, IL-1β, and TNF-α levels. The gut-liver axis modulation was achieved by strengthening intestinal barrier function (ZO-1, Occludin, Claudin-1), restoring microbial balance, and promoting the production of advantageous metabolites. DOP intervention increased the relative abundance of Ligilactobacillus, Akkermansia, and Lachnospiraceae_NK4A136_group to reverse gut microbiota disorder, and improved acetic acid, butyric acid and propionic acid levels. DOP alleviated alcohol-induced liver injury by modulating gut microbiota composition, enhancing short-chain fatty acids (SCFAs) production, restoring intestinal barrier integrity, and curbing LPS leakage. This study demonstrated the potential of DOP as a functional food for AALI mitigation while establishing the foundation for therapeutic product development.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"144719"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling in-kernel starch multiscale structure and nutritional profiles of thermal remodeling whole grain black tartary buckwheat 热重塑全粒黑苦荞籽粒内淀粉多尺度结构及营养特性的研究

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144730

Mengna Zhang , Pinjia Chen , Fengwei Yang , Tian Zhao , Jie Wang , Shiqi Li , Zhigang Chen , Jianchang Jin , Jinyan Gong

{"title":"Unraveling in-kernel starch multiscale structure and nutritional profiles of thermal remodeling whole grain black tartary buckwheat","authors":"Mengna Zhang , Pinjia Chen , Fengwei Yang , Tian Zhao , Jie Wang , Shiqi Li , Zhigang Chen , Jianchang Jin , Jinyan Gong","doi":"10.1016/j.ijbiomac.2025.144730","DOIUrl":"10.1016/j.ijbiomac.2025.144730","url":null,"abstract":"<div><div>Thermal treatments can improve the processing suitability and nutritional utilization of cereals. In this study, the effects of three thermal treatments, namely heat fluidization, microwave, and baking, on the physical, nutritional, and structural properties of whole grain black Tartary buckwheat (BTB), as well as the multiscale structures and digestive characteristics of in-kernel starches, were evaluated. Thermal treatments induced grain expansion, surface cracking, and porous internal structures, with heat fluidization causing the most significant reduction (32.06 %) in kernel hardness. Heat fluidization also enhanced rutin extractability by 68.00 % and antioxidant activities. Volatile compound analysis revealed heat fluidization reduced aldehyde and hydrocarbon levels, especially reducing hexanal from 27.06 to 3.86 mg/kg. For in-kernel starches, heat fluidization increased cold-water viscosity (56-fold), disrupted A-type crystallinity (relative crystallinity dropped to 8.49 %), reduced short-range ordered structure (R<sub>993/1018</sub> decreased by 2.21 %), and enhanced resistant starch content (33 %). The study highlighted heat fluidization as the most effective method for enhancing processing and nutritional properties of whole grain BTB, offering insights for developing nutrient-dense whole grain products.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144730"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PTPN2: Advances and perspectives in cancer treatment potential and inhibitor research PTPN2：肿瘤治疗潜力及抑制剂研究进展与展望

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144740

Yaru Zhang , Xiaocui Shuai , Yang Lei , Tingnan Ma , Tao Yuan , Shaomin Zhu , Lei Zhong

{"title":"PTPN2: Advances and perspectives in cancer treatment potential and inhibitor research","authors":"Yaru Zhang , Xiaocui Shuai , Yang Lei , Tingnan Ma , Tao Yuan , Shaomin Zhu , Lei Zhong","doi":"10.1016/j.ijbiomac.2025.144740","DOIUrl":"10.1016/j.ijbiomac.2025.144740","url":null,"abstract":"<div><div>Protein tyrosine phosphatase non-receptor 2 (PTPN2) is a well-established protein tyrosine phosphatase (PTP) that counterbalances protein tyrosine kinases (PTKs) activity by dephosphorylating tyrosine residues. Accumulating evidence demonstrates that dysregulated PTPN2 expression or activity regulates cancer biology, drives immune evasion, and confers resistance to immune checkpoint blockade therapy, establishing it as a compelling therapeutic target for cancer immunotherapy. Mechanistically, PTPN2 exerts immunosuppressive effects by dephosphorylating and negatively regulating immune response signaling, thereby dampening antitumor immunity and fostering an immunosuppressive tumor microenvironment. Recent breakthroughs in PTPN2 inhibitor development, such as small molecules, natural compounds, and PROTAC degraders, have shown promising efficacy in restoring immune-mediated tumor control. However, the anti-tumor therapy targeting PTPN2 still faces challenges, including its controversial role in some tumors, potential side effects, and the lack of highly effective and selective antagonists. In this comprehensive review, we synthesize the crucial functions of PTPN2 in tumor progression and immune regulation, systematically present the latest research progress of PTPN2 inhibitors, critically analyze unresolved hurdles in drug development, and provide strategic perspectives on addressing these challenges, with the hope of accelerating the clinical translation of PTPN2-targeted therapy from bench to bedside.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144740"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative unsaturated polyurethane for cardiac regeneration: Enhancing cytocompatibility and gene expression through gelatin modification 用于心脏再生的创新不饱和聚氨酯：通过明胶修饰增强细胞相容性和基因表达

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144659

Reza Behnam , Mohammad Dinari , Tayebeh Behzad

{"title":"Innovative unsaturated polyurethane for cardiac regeneration: Enhancing cytocompatibility and gene expression through gelatin modification","authors":"Reza Behnam , Mohammad Dinari , Tayebeh Behzad","doi":"10.1016/j.ijbiomac.2025.144659","DOIUrl":"10.1016/j.ijbiomac.2025.144659","url":null,"abstract":"<div><div>Developing a biomimetic platform that can effectively support myocardial repair and regeneration after a heart attack remains a major challenge for researchers. Polyurethanes (PU), known for their versatile properties, are increasingly being recognized as promising materials for creating cardiac patches. This research study developed the synthesis of a novel unsaturated chain extender via the Baylis-Hillman reaction for incorporation into the backbone of PU to create the unsaturated bridge for post-modification response. Thiol-ene chemistry performed post-modification of unsaturated hard domains of PU with thiolated gelatin as another reactant. Modification of the hard segment domains of PU with gelatin can have a synergistic effect on fabricating a new platform for repairing cardiac tissue. The films were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Nuclear Magnetic Resonance (NMR), mechanical tests, in vitro degradation rate, water contact angle measurement, and swelling measurement. Metabolic activity and cytocompatibility of the cells onto the films were assessed by MTT assay. An investigation of cardiac-specific marker expression was conducted using immunofluorescence staining and quantitative real-time PCR. The findings showed that none of the substances were cytotoxic to cardiomyocytes, and that the expression of cadiac-specific genes, such as Troponin T, GATA4, Connexin43, and Alpha-SMA, was higher than that of the control group.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144659"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulose nanocrystal-based intelligent hydrogels: Innovations, challenges, and prospective application in advanced wound healing 基于纤维素纳米晶体的智能水凝胶：创新、挑战和在高级伤口愈合中的应用前景

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144752

Saah Siaffa Martin , Lu Gan , Lingli Zhang , Xurui Yang , Zhongbiao Tan , Hao Shi , Lingfeng Long , Hongliang Li

{"title":"Cellulose nanocrystal-based intelligent hydrogels: Innovations, challenges, and prospective application in advanced wound healing","authors":"Saah Siaffa Martin , Lu Gan , Lingli Zhang , Xurui Yang , Zhongbiao Tan , Hao Shi , Lingfeng Long , Hongliang Li","doi":"10.1016/j.ijbiomac.2025.144752","DOIUrl":"10.1016/j.ijbiomac.2025.144752","url":null,"abstract":"<div><div>Cellulose nanocrystals (CNCs) have emerged as a transformative material in biomedical engineering due to their exceptional mechanical properties, high aspect ratio, and biocompatibility. Recent advances in CNC-based smart hydrogels show great potential in wound care through responsive drug delivery, moisture retention, and infection control. This review critically evaluates CNC-reinforced hydrogels' synthesis, functionalization, and biomedical applications, emphasizing their role in addressing chronic wound healing challenges. Despite promising results, clinical use is limited by scalability, cost, and long-term biocompatibility. Future research should optimize sustainable CNC extraction, integrate smart sensing, and explore cellular mechanisms. Collaboration with industry and pilot projects will provide insights, while workshops can train professionals in smart sensing and CNC optimization. Incorporating Internet of Things (IoT) sensors in CNC machines enables real-time monitoring of key parameters like temperature, vibration, and tool wear, facilitating predictive maintenance and process optimization through data analytics. Developing closed-loop control systems to adjust machining parameters based on real-time data enhances precision and reduces waste. CNC extraction optimization should include determining ideal process parameters, exploring advanced tooling materials, and using simulation software to streamline machining processes. This comprehensive analysis underscores the potential of CNC-based hydrogels to redefine regenerative medicine and personalized wound care.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144752"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Causal relationship between 91 circulating inflammatory proteins and gynecological diseases: A two-sample bidirectional Mendelian randomization study 91种循环炎症蛋白与妇科疾病的因果关系：一项双样本双向孟德尔随机研究

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144729

Jie-Lin Wang , Ping-An Zhang , Jing Yuan , Xiang-Chun Huang , Jia-Chen Cheng , Hai-Juan Bao , Jing Li , Shuo Chen

{"title":"Causal relationship between 91 circulating inflammatory proteins and gynecological diseases: A two-sample bidirectional Mendelian randomization study","authors":"Jie-Lin Wang , Ping-An Zhang , Jing Yuan , Xiang-Chun Huang , Jia-Chen Cheng , Hai-Juan Bao , Jing Li , Shuo Chen","doi":"10.1016/j.ijbiomac.2025.144729","DOIUrl":"10.1016/j.ijbiomac.2025.144729","url":null,"abstract":"<div><div>Gynecological diseases pose major public health challenges due to their detrimental impact on women's quality of life and fertility. While growing evidence implicates inflammatory proteins in the pathogenesis of these conditions, their causal roles remain unclear. In this study, we conducted a bidirectional two-sample Mendelian randomization (TSMR) analysis to explore causal relationships between 91 circulating inflammatory proteins and 17 gynecological diseases in a discovery cohort. We identified nine protein–disease pairs with potential causal associations, three of which remained significant after Bonferroni correction, including IL-1α with increased risk of cervical cancer, MMP10 with reduced risk of endometrial cancer, and MCP-1 with increased susceptibility to ovarian benign neoplasms. A meta-analysis incorporating data from an independent replication cohort further suggested that IL-1α increases the risk of cervical cancer. To validate these findings, we performed transcriptomic analysis using the TCGA database and confirmed protein-level expression through Western blot analysis of clinical samples. Collectively, these results provide novel insights into the inflammatory mechanisms underlying gynecological diseases and underscore IL-1α as a promising causal biomarker and potential therapeutic target for cervical cancer.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144729"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal-polyphenol nanoparticles-loaded carboxymethyl cellulose-based microneedle for promoting the healing of diabetic wounds 金属多酚纳米颗粒负载羧甲基纤维素微针促进糖尿病伤口愈合

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144716

Miaoyan Ren , Nan Wang , Liuyan Pu , Xiao-kun Ouyang , Jingfei Mao

{"title":"Metal-polyphenol nanoparticles-loaded carboxymethyl cellulose-based microneedle for promoting the healing of diabetic wounds","authors":"Miaoyan Ren , Nan Wang , Liuyan Pu , Xiao-kun Ouyang , Jingfei Mao","doi":"10.1016/j.ijbiomac.2025.144716","DOIUrl":"10.1016/j.ijbiomac.2025.144716","url":null,"abstract":"<div><div>Inflammation and infection are significant barriers to the healing of diabetic wounds. Infectious wounds are often associated with the formation of bacterial biofilms, which impede the penetration of conventional drugs. In this study, we developed a novel metal-polyphenol composite nanoparticle (EGCG-Cu<sup>2+</sup> NPs) by combining epigallocatechin gallate (EGCG) with Cu<sup>2+</sup> and incorporated these nanoparticles into microneedles (MNs) made from polyvinylpyrrolidone (PVP) and sodium carboxymethyl cellulose (CMC<img>Na). The resulting EGCG-Cu<sup>2+</sup> MNs exhibited remarkable anti-inflammatory and antibacterial properties, achieving over 95 % inhibition against <em>S. aureus</em> and <em>P. aeruginosa</em>. Moreover, they effectively scavenged reactive oxygen species (ROS), inhibited the M1 polarization of macrophages, and promoted their M2 polarization. In diabetic wound infection models, the EGCG-Cu<sup>2+</sup> MNs enhanced collagen deposition and angiogenesis, resulting in over 92 % wound closure within 14 days. This study provides a significant theoretical basis for innovative treatment strategies for infectious diabetic wounds, highlighting its promising clinical application potential.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144716"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Archaeal tyrosinase as a versatile biocatalyst for lignin-derived aromatic compounds valorization 古生菌酪氨酸酶作为木质素衍生芳香化合物增值的多功能生物催化剂

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144669

Seul Hoo Lee , Eungsu Kang , Yoo Seong Choi , Kyung-Jin Kim

{"title":"Archaeal tyrosinase as a versatile biocatalyst for lignin-derived aromatic compounds valorization","authors":"Seul Hoo Lee , Eungsu Kang , Yoo Seong Choi , Kyung-Jin Kim","doi":"10.1016/j.ijbiomac.2025.144669","DOIUrl":"10.1016/j.ijbiomac.2025.144669","url":null,"abstract":"<div><div>Biomass-derived aromatic compounds, including those obtained from lignin (which is the most abundant aromatic biopolymer on Earth), are valuable for sustainable chemical production. Various lignin-degrading approaches have been developed to cleave recalcitrant bonds. The incorporation of biocatalysts that operate under environmentally friendly and mild conditions with high substrate specificity is considered one of the emerging strategies for lignin valorization. In this study, an archaeal tyrosinase (Tyr-CNK), derived from the marine archaeon <em>Candidatus nitrosopumilus koreensis</em>, is characterized as a versatile biocatalyst for lignin biodegradation and valorization, based on kinetic studies, protein structure determination, and analysis. Notably, the extremely shallow active site pocket and the unique noncanonical caddy domain, which facilitate efficient copper incorporation without obstructing the active site, collectively empower Tyr-CNK with remarkable catalytic efficiency toward various lignin model compounds, such as <em>p</em>-coumaric acid, 4-phenoxyphenol, 4-(benzyloxy)phenol, and guaiacyl glycerol-β-guaiacyl ether. Together with molecular docking simulations, these catalytic and structural features indicate that Tyr-CNK serves as an efficient biocatalyst for the hydroxylation and oxidative degradation of lignin-derived phenolic compounds. Given its versatility, efficiency, and structural uniqueness, Tyr-CNK demonstrates great promise for expanding the catalytic repertoire for biomass conversions and offering new opportunities in sustainable biocatalysis, enzymatic and microbial biodegradation and biomass valorization.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144669"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Based OSA starch-based microcapsules from Pickering emulsion templates for functional sustained release of pepper essential oil. 基于OSA淀粉基微胶囊的功能性辣椒精油缓释皮克林乳液模板。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144715

Yitong Wang, Hui Teng, Ruizi Zhou, Shuxian Bai, Chao Ai, Lei Chen

{"title":"Based OSA starch-based microcapsules from Pickering emulsion templates for functional sustained release of pepper essential oil.","authors":"Yitong Wang, Hui Teng, Ruizi Zhou, Shuxian Bai, Chao Ai, Lei Chen","doi":"10.1016/j.ijbiomac.2025.144715","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.144715","url":null,"abstract":"<p><p>Pepper essential oil has the effect of anti-inflammatory, anti-oxidant and anti-bacterial activity. Enhance stability and solubility of oil using food-compatible Pickering emulsions. In this study, pepper essential oil of terpenes accounted for 76.11 % and the alcohols accounted for 6.58 %. At the same time, the relatively high fatty acid components were 29.50 % linoleic acid and 26.89 % palmitic acid in pepper essential oil. Then, Pickering emulsion incorporating octenyl succinic acid starch (OSA starch) was employed for the encapsulation of the essential oil. Under prepared conditions of Pickering emulsion, the minimum particle size was 2.11 ± 0.12 μm, the droplet morphology was smooth and uniform. Pickering emulsion has high stability within 30 d, which also can effectively delay the aroma release rate of essential oils. The emulsion showed higher antioxidant effect than the pure pepper essential oil. The same trend was observed with strong anti-bacterial and anti-inflammatory activity. Microcapsules were successfully prepared to reduce free fatty acid production. The results obtained demonstrated that the pepper essential oil contained with OSA starch Pickering emulsion has enormous potential for application in the food industry.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"144715"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0