International Journal of Biological Macromolecules最新文献

{"title":"A complex interplay between alginate/pectin and rice varieties in determining the starch digestibility of reconstituted rice beads","authors":"Senbin Cao,&nbsp;Cheng Li","doi":"10.1016/j.ijbiomac.2025.143809","DOIUrl":"10.1016/j.ijbiomac.2025.143809","url":null,"abstract":"<div><div>Non-starch polysaccharide (NSP) encapsulated rice flour is a novel type of rice analogue, while the influence of different NSPs and rice varieties on starch digestibility remains unknown. Therefore, three rice varieties with different structures were encapsulated by alginate or pectin, and their digestibility and textures were investigated with regards to the alterations of their multi-scale structures during digestion. An approximately 1.9 times difference of enzymatic binding rate constant and 10 % difference of the maximum starch digested percentage were found among different rice beads. Nanjing rice beads showed slower starch digestibility when encapsulated by alginate, while Panjin and Subei rice showed the opposite. Alginate encapsulated rice beads showed a harder texture than pectin encapsulated ones, although the texture also depended on rice varieties. Mechanistically, amylopectin chains with a peak DP around 15 from Nanjing rice were proposed to interact with alginate, thus reducing its digestibility. In comparison, the large pores formed in pectin encapsulated Panjin and Subei rice beads after cooking promoted the re-association of starch molecules into more ordered structure and thus reduced digestibility. These results suggest that careful selection of both NSP types and rice varieties is required to develop rice beads/analogues with desirable starch digestibility.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143809"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between Bacteroides and HG-type pectins with different molecular weights","authors":"Song Li ,&nbsp;Xiaomao Peng ,&nbsp;Zengbo Wang ,&nbsp;Chunhua Chen ,&nbsp;Xin Li ,&nbsp;Qixing Nie ,&nbsp;Xiaojun Huang ,&nbsp;Shuigen Bian ,&nbsp;Junyi Yin ,&nbsp;Steve W. Cui ,&nbsp;Huizi Tan ,&nbsp;Shaoping Nie","doi":"10.1016/j.ijbiomac.2025.142363","DOIUrl":"10.1016/j.ijbiomac.2025.142363","url":null,"abstract":"<div><div>Pectins, a complex class of polysaccharides, are prominently represented by HG-chains, which are both abundant and extensively studied. <em>Bacteroides</em> species exhibit a remarkable ability to metabolize plant- and animal-derived polysaccharides, including the degradation of HG-type pectins through polysaccharide utilization locus (PUL). However, the effects of structurally diverse HG-type pectins on PUL expression, metabolite production, and metabolic pathways of <em>Bacteroides</em> remain unclear. Addressing this, this study chose HG-type pectins with different molecular weights from citrus (CP) and pomelo (PP), identified <em>Bacteroides</em> species capable of utilizing these pectins, studied the resulting metabolites through non-targeted metabolomics coupled with short-chain fatty acids analysis, and examined the activation of PUL and metabolic pathways by transcriptomic studies. The results showed that <em>Bacteroides thetaiotaomicron</em> A4 and <em>Bacteroides caccae</em> K9 could utilize HG-type pectins with different molecular weights. The production of propionic acid by <em>Bacteroides thetaiotaomicron</em> A4 was significantly affected by the molecular weight of pectins. Utilizing CP, <em>B. thetaiotaomicron</em> A4 enriched metabolites such as carbohydrates, amino acids, peptides, amines, and significantly enhanced pathways such as sphingolipid metabolism and drug metabolism (other enzymes) through PUL75, PUL63, and PUL55. In contrast, when using PP, <em>B. thetaiotaomicron</em> A4 enriched similar metabolites and further upregulated pathways related to sphingolipid metabolism and pyrimidine metabolism. The molecular weight of HG-type pectins differentially affected the expression of carbohydrate-active enzymes and metabolic pathways, resulting in different metabolite profiles. This study aims to contribute to the understanding of structure-activity relationship between pectins and gut microbiota and to inform precision nutrition strategies.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142363"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Exosome-loaded hyaluronic acid hydrogel composite with oxygen-producing 3D printed polylactic acid scaffolds for bone tissue repair and regeneration” [Int. J. Biol. Macromol. 274 (2024) 132970]","authors":"Yifan Zhang ,&nbsp;Min Fang ,&nbsp;Junbin Zhu ,&nbsp;Ting Li ,&nbsp;Na Li ,&nbsp;Bo Su ,&nbsp;Guo-Dong Sun ,&nbsp;Lihua Li ,&nbsp;Changren Zhou","doi":"10.1016/j.ijbiomac.2025.142032","DOIUrl":"10.1016/j.ijbiomac.2025.142032","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142032"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the structural characterization of Premna microphylla Turcz polysaccharides and their improvement effect on the properties of chitosan composite gel","authors":"Bingmin Wu ,&nbsp;Yong Zhu ,&nbsp;Yao Liu ,&nbsp;Yu Wang ,&nbsp;Yuhe Dong ,&nbsp;Jihang Chen ,&nbsp;Tian Zhong","doi":"10.1016/j.ijbiomac.2025.143015","DOIUrl":"10.1016/j.ijbiomac.2025.143015","url":null,"abstract":"<div><div>Bioactive polysaccharide composite gels provide strategy for improving the defects of chitosan (CS) gel. The aim of this study was to characterize the structure of <em>Premna microphylla</em> Turcz polysaccharide (PMTP) and investigate the improvement of different PMTP concentrations (4, 6, 8, 10, and 12 wt%) on the PMTP/CS composite gels' properties. PMTP is an acidic heteropolysaccharide with →4)-α-D-GalpA-(1→ and →4)-α-D-Galpa-6-Ome-(1→ as the backbone structure. And PMTP transformed the gel's framework from the lamellar structure of CS gel to 3D porous network constructed by connected nanofibers. This resulted in the increase of surface area from 11.28 to 89.72 m²/g and reduction of pore size from 5.76 to 0.52 μm. Moreover, the mechanical properties of PMTP/CS composite gel was significantly improved 17.9 times higher than that of CS gel. Accordingly, the water holding, swelling, rheology and thermal stability of PMTP/CS gel were further improved. Study on gelation mechanism proved that the formation of composite gel's 3D network was mainly dominated by electrostatic interaction. Finally, Caco-2 cell assay in vitro confirmed the excellent cytocompatibility of PMTP/CS gel. In conclusion, this work provides a scientific reference for the design of bioactive polysaccharide composite gel for food engineering field.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 143015"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stabilization of high internal phase emulsions by combination of polysaccharide and protein: Synergistic efficiency, mechanism, and application","authors":"Yuqian Zhu ,&nbsp;Yubo Zhu ,&nbsp;Jingchen Yang,&nbsp;Yanbo Wang,&nbsp;Chen Tan","doi":"10.1016/j.ijbiomac.2025.143544","DOIUrl":"10.1016/j.ijbiomac.2025.143544","url":null,"abstract":"<div><div>This review presents recent advances in the combined use of protein and polysaccharide for synergistic stabilization of high internal phase emulsions (HIPEs). Four types of protein-polysaccharide systems that can be used as stabilizes for HIPEs are discussed, including non-covalent complexes, covalent conjugates, core-shell nanoparticles, and ternary complexes. These binary/ternary systems exhibit favorable performance in the construction of stable HIPEs, protection and controlled delivery of bioactive compounds. We discuss various material sources, formation mechanism, and physicochemical characteristic of each system. The challenges associated with the appropriate selection of wall materials, interaction with digestive systems, and <em>in vivo</em> biological fate of HIPEs are also highlighted. This review could help comprehensively understand the synergistic efficiencies of protein and polysaccharide on HIPEs for the functional food applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143544"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel bioink derived from low methoxyl pectin, gelatin and aloe vera as natural biomaterials for fabricating scaffolds encapsulated with living cells by 3D bioprinting","authors":"Jirasak Jitpibull ,&nbsp;Thunyaporn Ravangnam ,&nbsp;Nalinrat Petpiroon ,&nbsp;Wichaya Kalaithong ,&nbsp;Kanittha Boonpavanitchakul ,&nbsp;Sasitorn Aueviriyavit ,&nbsp;Wiyong Kangwansupamonkon","doi":"10.1016/j.ijbiomac.2025.143364","DOIUrl":"10.1016/j.ijbiomac.2025.143364","url":null,"abstract":"<div><div>In recent years, 3D bioprinting has emerged as a promising technology for developing complex tissue structures, precisely controlled and designed <em>via</em> computer systems to create intricate organ model. This study focuses on the development of a bioink composed of living cells and hydrogel, specifically using low methoxyl pectin (LMP) combined with gelatin to enhance the properties of bioink for tissue engineering applications. Additionally, aloe vera (AV) dry gel was incorporated into the bioink to promote cell proliferation. The rheological properties of the developed hydrogels were evaluated, revealing shear-thinning and thixotropic behaviors suitable for 3D bioprinting applications. FT-IR and TGA analyses were performed to investigate the chemical and thermal properties of the hydrogels, indicating interactions between the constituents. The printability assessment demonstrated that the developed hydrogels (LMP/Gel, LMP/Gel-1AV, LMP/Gel-2AV) were able to maintain accurate shapes with over 90 % precision. Morphological observation of the dried hydrogel revealed a porous structure with interconnected pores, ensuring nutrient diffusion throughout the hydrogel. Furthermore, the developed bioinks demonstrated biodegradability, low toxicity, and the successful formation of an epidermal-like structure, further highlighting their potential for tissue engineering applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143364"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of glucans from diverse sources and their influence on fat and carbohydrate absorption, digestion in vitro, and glucose tolerance in vivo","authors":"Yao Liu ,&nbsp;Juan Dai ,&nbsp;Huajuan Song ,&nbsp;Zheng Zeng ,&nbsp;Mengxia Wu ,&nbsp;YuKun Huang ,&nbsp;Lijun Wang ,&nbsp;Pengfei Chen ,&nbsp;Chisong Zhang ,&nbsp;Juan Zhao ,&nbsp;Xiao Yang ,&nbsp;Xianggui Chen","doi":"10.1016/j.ijbiomac.2025.142025","DOIUrl":"10.1016/j.ijbiomac.2025.142025","url":null,"abstract":"<div><div>Glucan, a recognized prebiotic primarily derived from fungi, bacteria, and plants, possesses significant nutritional value and biological activity. It serves as a thickener and emulsifier stabilizer, enhancing the texture and sensory properties of food. This study aimed to compare the inhibitory effects of glucans from yeast, oats, and bacteria on energy intake by characterizing their physicochemical properties and evaluating their impact on fat and glucose adsorption, starch and fat digestion <em>in vitro</em>, and glucose tolerance <em>in vivo</em>. The findings revealed that despite sharing similar active groups, the glucans exhibited distinct structures, viscosities, water solubilities, thermal degradation behaviors, and micromorphologies. All three sources demonstrated effectiveness in adsorbing fat and glucose, inhibiting starch and fat digestion, and improving glucose tolerance in mice, albeit with notable differences. Among these, Salecan glucan, derived from bacterial sources, exhibited superior performance in fat absorption, inhibition of starch and fat digestion, and enhancement of glucose tolerance. This is likely attributable to its higher viscosity, greater water solubility, and linear molecular structure. These results highlight the functional significance of glucans from different sources and underscore their potential application in developing functional foods aimed at managing energy intake.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142025"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional loofah-inspired microfibrillated cellulose aerogel with excellent thermal insulation and sorption properties","authors":"Wei Qin ,&nbsp;Huimin Zhang ,&nbsp;Ju Wei ,&nbsp;Yitong Chen ,&nbsp;Yongping Liao ,&nbsp;Xiaoxiao Wei ,&nbsp;Meifeng Guo ,&nbsp;Jialing Huang ,&nbsp;Hua Shen","doi":"10.1016/j.ijbiomac.2025.143737","DOIUrl":"10.1016/j.ijbiomac.2025.143737","url":null,"abstract":"<div><div>Biomass aerogels have garnered increasing attention recently due to their wide-ranging applications. Yet, developing cost-effective and versatile aerogel materials remains challenging, as conventional biomass material production processes are complex and environmentally unfriendly. Loofah fiber, a porous cellulose fiber with a natural three-dimensional structure, has been recognized as a new sustainable resource in recent years. In this study, we adopted an innovative approach to fabricate a structurally stable and controllable loofah-derived cellulose aerogel by directly using natural agricultural fibers through simple mechanical treatment and freeze-drying. The achieved multifunctional aerogels exhibited high porosity (99.2 %), hydrophobicity (140.1°), self-cleaning, good mechanical performance, outstanding sorption capacity (40–190 g/g of oil and organic solvents) and thermal insulation (0.030 W/(m·K)) properties. Meanwhile, the aerogel also possessed excellent economy, reusability, and sustainability. This study paves the way for designing next-generation natural biomass-based sorbing and thermal insulation porous materials with great potential for multifunctional applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143737"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three polysaccharides from Roughhaired Holly Root with immune-enhancing activity via activation of TLR receptor","authors":"Rong Huang ,&nbsp;Xiaoli Huang ,&nbsp;Weining He ,&nbsp;Zhangfeng Zhong ,&nbsp;Haiqin Li ,&nbsp;Qiu Li","doi":"10.1016/j.ijbiomac.2025.142266","DOIUrl":"10.1016/j.ijbiomac.2025.142266","url":null,"abstract":"<div><div>In this study, we isolated three acidic polysaccharide components (IAPS-1, IAPS-2, and IAPS-3) from Rough-haired Holly (<em>Ilex asprella</em>) roots and systematically evaluated their immunomodulatory activity in murine macrophages. The polysaccharides were structurally characterized using integrated chromatographic and spectroscopic techniques. Subsequent bioactivity screening revealed that IAPS-1 demonstrated stronger immunomodulatory effects compared to IAPS-2 and IAPS-3. Mechanistically, in vitro experiments showed that IAPS-1 significantly upregulated TLR4-mediated expression of pro-inflammatory cytokines in RAW 264.7 macrophages. Furthermore, in cyclophosphamide-induced immunosuppressed mice, IAPS-1 administration restored immune organ indices and elevated serum levels of critical inflammatory mediators. Collectively, our findings identify IAPS-1 as a well-defined composition polysaccharide with immunomodulatory activity via TLR4 signaling, highlighting its potential as a therapeutic candidate for immune dysfunction.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142266"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic profiling of polysaccharides from Leccinum crocipodium (Letellier.) Watliag stem fermented by Bacteroides thetaiotaomicron and their immunomodulatory effects","authors":"Yingrun Fan ,&nbsp;Tingting Zheng ,&nbsp;Shuangmin Liang ,&nbsp;Yun Niu ,&nbsp;Zhichao Xiao ,&nbsp;Jiangping Fan","doi":"10.1016/j.ijbiomac.2025.142026","DOIUrl":"10.1016/j.ijbiomac.2025.142026","url":null,"abstract":"<div><div><em>Leccinum crocipodium</em> (Letellier.) Watliag has attracted increasing attention for their biological activity. In this study, the active polysaccharide components (LCSP11 and LCSP22) extracted from the stem of L. <em>crocipodium</em> (Letellier.) Watliag were investigated to assess structural characterization of LCSP11 and LCSP22, their effects on the metabolic profile of <em>Bacteroides thetaiotaomicron</em>, and the immune activities of the resulting fermentation products. The results showed that LCSP11 and LCSP22 were a type of heteropolysaccharide and amorphous state with good stability, which displayed molecular aggregation in aqueous solutions. LCSP11 and LCSP22 were effectively fermented by <em>Bacteroides thetaiotaomicron</em>, producing a variety of microbial metabolites, including organic acids and derivatives (30.13 %), lipids and lipid-like molecules (21.33 %), and organoheterocyclic compounds (17.45 %). Multiple differential metabolites were identified in the fermentation products (F11 and F22), with significant accumulation of peptides, amino acids, nucleotides, steroids, and fatty acids, such as murabutide and L-cystine. KEGG pathway analysis identified six enriched metabolic pathways in F11 and five in F22, with the histidine metabolic pathway significantly enriched in F11. Furthermore, LCSP22 fermentation by <em>Bacteroides thetaiotaomicron</em> produced short-chain fatty acids, including acetic acid, propionic acid, isovaleric acid, and caproic acid. Cellular experiments suggested that these fermentation metabolites exhibited immunoactivating effects on RAW264.7 cells, significantly enhancing phagocytic capacity and promoting the secretion of nitric acid (NO) and cytokines, including TNF-α, IL-6, and IL-2. These results provide new insights into the immunomodulatory activities of polysaccharides from the stem of L. <em>crocipodium</em> (Letellier.) Watliag fermented by <em>Bacteroides thetaiotaomicron</em> and broadens the potential applications of this natural resource in food, nutrition, and biomedicine.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142026"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}