International Journal of Biological Macromolecules最新文献

{"title":"Inhibitory activity and mechanisms of chitosan against Fusarium avenaceum, a pathogen causing Angelica root rot disease.","authors":"Yaya Cheng, Saimai Ma, Jianmei Dong, Wenwen Zhang, Yanjun Ma, Aimei Zhang, Hai Peng, Fujun Han, Weibao Kong","doi":"10.1016/j.ijbiomac.2025.140249","DOIUrl":"10.1016/j.ijbiomac.2025.140249","url":null,"abstract":"<p><p>The polysaccharide chitosan possesses broad-spectrum antimicrobial properties and has proven effective in controlling various postharvest diseases in fruits. Nevertheless, the fundamental mechanisms underlying its action remain unclear. In this study, the antifungal effects of chitosan with different molecular weights against Fusarium avenaceum, a pathogen causing root rot in Angelica sinensis, were evaluated. Additionally, the potential mechanisms of these effects were explored at the microstructural and transcriptomic levels. Notably, low-molecular-weight chitosan (20 kDa) exhibited superior antifungal activity when compared to high-molecular-weight chitosan (500 kDa and 1000 kDa). The half-maximal inhibitory concentration (IC₅₀) of 20, 500, and 1000 kDa chitosan were 0.2103, 0.2183, and 0.2707 g/L, respectively. Morphological and physiological experiments demonstrated that chitosan can inhibit the growth of F. avenaceum by decreasing spore germination, destroying mycelial morphology and microstructure, and promoting the release of intracellular electrolytes. RNA sequencing revealed considerable changes in the transcriptomic profile of F. avenaceum after chitosan treatment, with 2030 genes being differentially expressed. Subsequent KEGG pathway analysis demonstrated that genes associated with translation, human diseases, and transcription were upregulated in F. avenaceum after chitosan treatment. In contrast, genes associated with carbohydrate and amino acid metabolism, cellular processes, exogenous substance degradation and metabolism, and the metabolism of cofactors and vitamins were downregulated. Collectively, these results indicated that chitosan may influence the growth of F. avenaceum by disrupting protein biosynthesis and key metabolic pathways. These findings highlight the substantial potential of chitosan as an alternative agent for the management of fungal diseases in plants used in Chinese herbal medicine.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140249"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of dsDNA binding, enzyme inhibition, antioxidant activities, and molecular docking studies of taxifolin, daidzein, and S-equol.","authors":"Derya Kılıçaslan","doi":"10.1016/j.ijbiomac.2025.140314","DOIUrl":"10.1016/j.ijbiomac.2025.140314","url":null,"abstract":"<p><p>This study investigated the binding mechanism of taxifolin (TA), daidzein (DA), and S-equol (SQ) flavonoids with fish sperm double helix DNA (dsDNA) under the simulated physiological pH condition using UV-Vis and photoluminescence spectroscopy, as well as viscometric methods. Binding constants (K_b) for the flavonoids to dsDNA were determined as 1.8 × 10⁴ M^-1 for SQ, 1.6 × 10⁴ M^-1 for DA and 1.7 × 10⁴ M^-1 for TA, indicating moderate affinity. The groove binding mode was confirmed by competitive binding studies with ethidium bromide or rhodamine B, UV-Vis spectrophotometry and viscosity evaluation. Additionally, the compounds showed high cholinesterase (ChE) inhibitory activity, with TA being the most potent, particularly against BChE (IC₅₀ = 2.93 μM) and AChE (IC₅₀ = 6.42 μM). Antioxidant activities were also evaluated using DPPH and ABTS assays, with TA showing the lowest IC₅₀ values. Additionally, molecular docking studies were performed to assess the interactions and binding affinities of all compounds with AChE and BChE enzymes. As a result, the studied compounds were found to prefer minor groove binding. This research analyzed the contribution of the structure-activities of natural flavones in terms of their biological properties, which is important for their future application against diseases.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140314"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocomposite TiO₂/ZnO/chitosan by method sol-gel for self-cleaning application.","authors":"R Novra Gabriela, Heryanto Heryanto, Dahlang Tahir","doi":"10.1016/j.ijbiomac.2025.140076","DOIUrl":"10.1016/j.ijbiomac.2025.140076","url":null,"abstract":"<p><p>TiO₂/ZnO/Chitosan coated cotton fabric as a self-cleaning, which has been synthesized by various concentrations of TiO₂: 0.5 g, 1 g, and 2 g through the sol-gel method at pH 9. The self-cleaning test was conducted on TiO₂/ZnO/Chitosan-coated cotton fabric samples by irradiating for 15 h using UVA-UVB lamps with clothing stain dye. TiO₂/ZnO/Chitosan composite's structural properties were analyzed from X-ray diffraction (XRD) spectra, chemical bonding by Fourier Transform Infrared (FTIR), and bandgap by quantitative analysis from UV-visible spectroscopy. The XRD diffraction peaks showed a slight shift to the right, except for the sample with the highest TiO₂ concentration, which showed a more significant shift. FTIR spectra showed the presence of Ti-O-Ti bonds at wavenumbers 500 cm^-1 - 700 cm^-1, which identified the presence of TiO₂, and at wavenumber 3485 cm^-1, which was used for stretching-OH and -NH₂ of chitosan. The band gaps were 5.64 eV, 5.63 eV, and 5.58 eV for TiO₂: 0.5 g, 1 g, and 2 g, respectively. The self-cleaning test showed that the best results were in the TiO2 sample with a concentration of 2 g at pH 9, where the dye successfully disappeared after exposure to UVA-UVB lamps for 15 h of irradiation.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140076"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of interpenetrating networks from chitosan and poly(hydroxypropyl methacrylate) or p(hydroxyethyl methacrylate) for controlled release of doxorubicin and curcumin: Investigation of potential use in wound dressing.","authors":"Fatemeh Shakouripour, Ali Olad, Gulay Bayramoglu","doi":"10.1016/j.ijbiomac.2025.140929","DOIUrl":"10.1016/j.ijbiomac.2025.140929","url":null,"abstract":"<p><p>The IPNs hydrogel films based on chitosan (CS), 2-hydroxyethyl methacrylate (HEMA), and 2-hydroxypropyl methacrylate (HPMA) were prepared, and their potential for drug delivery and wound dressing was evaluated. The characterizations of the IPNs were examined through swelling tests, FTIR, DSC, SEM, mechanical properties, and BET analyses. The percent swelling of the CS/p(HEMA)1 and CS/p(HPMA)1 were obtained as 240 % and 110 %, respectively. The release behavior of prepared hydrogel formulations was investigated in two different pH values for DOX and CUR at pH 5.5 and 7.4, respectively, at varying drug concentrations. In vitro, drug release profiles revealed a time-dependent release pattern, with a maximum release observed at 48 h for all formulations. Among the IPNs, CS/p(HEMA)1 formulation containing CS/HEMA in a 1:1 ratio showed the highest drug release rates of 76.0 % for doxorubicin and 75.5 % for curcumin. MTT assays revealed that the IPNs formulations exhibit enhanced interaction with drugs, leading to an improved drug release rate. A marked decrease in cell viability was observed as the concentration of both drugs increased for testing the ATCC-CRL 2451 leukemia cell line in the prepared formulations. These findings highlight the potential of these composite hydrogels as efficient drug delivery systems for wound dressing applications.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140929"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting JAK/STAT3 in glioblastoma cells using an alginate-PNIPAm molecularly imprinted hydrogel for the sustained release of ruxolitinib.","authors":"Alexandra-Iulia Bărăian, Lajos Raduly, Oana Zănoagă, Bogdan-Cezar Iacob, Lucian Barbu-Tudoran, Elena Dinte, Ioana Berindan-Neagoe, Ede Bodoki","doi":"10.1016/j.ijbiomac.2025.140025","DOIUrl":"10.1016/j.ijbiomac.2025.140025","url":null,"abstract":"<p><p>Glioblastoma (GBM) is a notoriously aggressive primary brain tumor characterized by elevated recurrence rates and poor overall survival despite multimodal treatment. Local treatment strategies for GBM are safer and more effective alternatives to systemic chemotherapy, directly tackling residual cancer cells in the resection cavity by circumventing the blood-brain barrier. Molecularly imprinted polymers (MIPs) are promising drug delivery systems due to their high-affinity binding cavities that enable tailored release kinetics. This study reports the development of a semi-synthetic polysaccharide MIP-based hydrogel intended for the post-surgical management of GBM. The biodegradable implant, made of calcium-crosslinked alginate-poly(N-isopropylacrylamide) graft copolymer, was designed for the sustained release of ruxolitinib (RUX) in the resection cavity, targeting the Janus kinase/Signal Transducer and Activator of Transcription-3 signaling pathway. The molecularly imprinted hydrogel demonstrated thermo-thickening and shear-thinning behavior, high entrapment efficiency of RUX (84.59 ± 0.73 %), and sustained release over 14 days, underscoring the advantages that molecular imprinting of the alginate matrix provides compared to conventional MIPs. The dose-dependent inhibitory effects of the imprinted hydrogel against U251 and A172 GBM cells were demonstrated by increased apoptosis, reduced confluence, colony formation, and delayed wound healing, whereas the non-imprinted hydrogel was biocompatible. The MIP hydrogel could be a safe and effective GBM treatment.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140025"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bioactive Rosa roxburghii Tratt fruit polysaccharide on the structure and emulsifying property of lactoferrin protein.","authors":"Wang Pingping, Du Yutong, Chai Xianghua, Chen Chun, Wu Kegang, Fu Xiong","doi":"10.1016/j.ijbiomac.2025.140016","DOIUrl":"10.1016/j.ijbiomac.2025.140016","url":null,"abstract":"<p><p>Lactoferrin protein (LF) is a natural protein with certain emulsifying ability, but is sensitive to be affected by environmental factors and has poor oxidative stability to be used as emulsifier. In this study, the emulsifying ability of LF was significantly improved after conjugation with Rosa roxburghii Tratt fruit polysaccharides (RTFP), and the emulsion stability mechanism of LF-RTFP conjugates (L-R) were elucidated through the utilization of CLSM (confocal laser scanning microscopy), interfacial tension, apparent viscosity, and protein adsorption rate. The emulsion stabilized by L-R showed the smaller particle size (17.17 ± 0.13 μm), which reduced by 51 % compared with that of LF. After conjugation with RTFP, the hydrophobic amino acids that are originally inside the structure of LF would be exposed on the protein surface. In addition, the protein adsorption rate of L-R stabilized emulsion interface was 62.70 ± 0.71 %, 2.4 times higher than that of LF. The optical microscopy and CLSM experiments indicated that the glycosylation with RTFP increased the bridged flocculation and further formed gel network structure in the emulsion stabilized by LF. These findings suggested that the novel emulsifier prepared by the Maillard reaction between LF and RTFP showed potential to stable emulsion.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140016"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Characterization and functional properties of walnut protein fibrils for enhanced bioaccessibility of CoQ10 and ALA\" [International Journal of Biological Macromolecules, Volume 285 (2025), 138171].","authors":"Xiaoyu He, Li Zhang, Minjie Cao, Yiwen Guo, Jie Mi, Zhu Zhu, Yuehao Song, Ming Chang, Ruijie Liu, Xingguo Wang","doi":"10.1016/j.ijbiomac.2025.139960","DOIUrl":"10.1016/j.ijbiomac.2025.139960","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"139960"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cobalt polygalacturonates and the pharmacological composition based on them: Preparation, properties and cytotoxicity.","authors":"Salima T Minzanova, Evgenii V Chekunkov, Anna V Khabibullina, Lubov G Mironova, Alexandra D Voloshina, Anna P Lyubina, Kirill V Kholin, Guliya R Nizameeva, Airat R Khamatgalimov, Vasili A Milyukov","doi":"10.1016/j.ijbiomac.2025.140377","DOIUrl":"10.1016/j.ijbiomac.2025.140377","url":null,"abstract":"<p><p>The aim of the present study was to obtain new metal complexes of citrus pectin with cobalt ions based on potassium polygalacturonate and to prepare a new pharmacological composition (PC) PGKCo: PGNaCo (1:1) with antitumor activity based on potassium cobalt polygalacturonate (PGKCo) and sodium cobalt polygalacturonate (PGNaCo). The study of the effect of PGKCo, PGNaCo and PC on the cell viability of tumor cell lines of different genesis in vitro showed that the obtained compounds are soluble in water and exhibit selective cytotoxic activity against the tumor cell lines of human lung carcinoma A549, breast adenocarcinoma MCF-7 and cervical carcinoma M-HeLa, with no significant toxic effect on normal human cells. The possible mechanism of action of the investigated PC on M-HeLa cancer cells was investigated. The mechanism of action of PC was found to be associated with cell cycle arrest in the G0/G1 phase and the induction of apoptosis through the mitochondrial pathway. The results obtained indicate the potential for the non-toxic compounds (PGKCo, PGNaCo and PC) to be developed as drugs for the complex treatment of oncologic diseases.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140377"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polysaccharides from Scrophularia ningpoensis Hemsl. improve reserpine-induced depression-like behavior by inhibiting HTR2A/HTR2C mediated AKT/GSK3β/β-catenin/CBP/BDNF signalling.","authors":"Ruhui Shen, Jian'an Wang, Yijin Zhao, Zhaojin Dang, Ke Zhang, Ming Li, Qian Yang, Li-Na Gao","doi":"10.1016/j.ijbiomac.2025.140445","DOIUrl":"10.1016/j.ijbiomac.2025.140445","url":null,"abstract":"<p><p>Scrophularia ningpoensis Hemsl. is a traditional Chinese medicine used to regulate blood sugar levels, immunity, etc. We previously isolated polysaccharides from S. ningpoensis Hemsl. (SNPS) and innovatively observed that SNPS exhibit antidepressant properties; however, the underlying mechanism is still unclear. Here, we employed network pharmacology to predict the potential targets and antidepressant mechanism of SNPS. Accordingly, we detected the effects of SNPS on monoamine neurotransmitter synthesis, metabolism, receptor expression and signal transduction in reserpine (RES)-treated mice using ELISA, HPLC-electrochemistry, metabonomics, Golgi-Cox staining and Western blotting. Finally, the mechanism of SNPS on key targets (HTR2A and HTR2C) was verified in vivo and in vitro. Results showed that SNPS ameliorated depression by restoring monoamine neurotransmitter homeostasis and hippocampal neurogenesis. SNPS reversed the depletion of 5-HT, NE and DA by activating the tryptophan (Trp)/5-HT and tyrosine (Tyr)/DA/NE metabolic pathways. SNPS decreased HTR2A and HTR2C contents, leading to the phosphorylation of AKT and GSK3β, followed by increases in β-catenin, CBP and BDNF levels. Mechanistically, SNPS reduced the levels of HTR2A and HTR2C proteins by inhibiting their mRNA transcription, rather than inducing protein degradation. In conclusion, by inhibiting the transcription of HTR2A and HTR2C, SNPS activated the AKT/GSK3β/β-catenin/CBP/BDNF pathway, thereby exerting dose-dependent antidepressant effects.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140445"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-performance silk fibroin/hyaluronic acid interpenetrating network hydrogel microneedles for diabetes management.","authors":"Jiahui Hua, Renyan Huang, Meng Yu, Renchuan You, Lu Wang, Shuqin Yan, Ying Huang, Qiang Zhang","doi":"10.1016/j.ijbiomac.2025.140357","DOIUrl":"10.1016/j.ijbiomac.2025.140357","url":null,"abstract":"<p><p>Hydrogel microneedles (MNs) gained more attentions for diabetes treatments owing to their biocompatibility and versatility. However, the inherent fragility and instability of hydrogels pose limitations on their efficacy in biomedical applications. To overcome this limitation, we developed interpenetrating network hydrogels (IPNs) by incorporating silk fibroin (SF) and methacrylated hyaluronic acid (HAMA). These hydrogels exhibit rapid formation, structural stability, mechanical robustness, and sustainability through photo-crosslinking without the need for crosslinking agents. The hydrogels demonstrated an average formation time of 86 ± 8 s and exhibited favorable elasticity, along with a high compressive stress at break of 70.9 ± 8.2 kPa. Additionally, the extensive proliferation and well-distributed network of human umbilical vein endothelial cells (hUVECs) on the microneedles' (MNs) surface underscored the high cytocompatibility and cell viability of the MNs. In a diabetic mouse model, the MNs were able to maintain normal blood glucose levels for approximately 6 h. The administration of insulin-loaded microneedles to diabetic mice resulted in glucose tolerance levels comparable to those of non-diabetic mice, indicating the efficacy of microneedle therapy in improving the glycemic condition of diabetic subjects. These hydrogel MNs possess a stable structure, can be rapidly fabricated, are sustainable, and hold significant potential for the clinical management of patients with diabetes mellitus.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140357"},"PeriodicalIF":7.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}