International Journal of Biological Macromolecules最新文献_第6页

Fabrication of alendronate doped sodium alginate composite hydrogel for enhanced adsorption of rare earth elements from wastewater 阿仑膦酸钠掺杂海藻酸钠复合水凝胶的制备及其对废水中稀土元素的强化吸附。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148161

Saeed Ahmed Memon, Wenting Li, Yun Wei

{"title":"Fabrication of alendronate doped sodium alginate composite hydrogel for enhanced adsorption of rare earth elements from wastewater","authors":"Saeed Ahmed Memon, Wenting Li, Yun Wei","doi":"10.1016/j.ijbiomac.2025.148161","DOIUrl":"10.1016/j.ijbiomac.2025.148161","url":null,"abstract":"<div><div>The rising demand for rare earth elements (REEs) in agriculture and industry highlights the risk of environmental contamination due to their inherent toxicity and potential for ecological accumulation. In this work, a simple and cost-effective alendronate modified sodium alginate composite hydrogel (SA@ANDS) was designed and successfully fabricated. Using the adsorption of REEs La (III) as a scenario, several parameters including pH, adsorbent dosage, time, initial concentration and temperature were systematically examined. Furthermore, isothermal adsorption was adopted to analyze the adsorption mechanism. The results revealed that the adsorption capacity of the adsorbent was 163.93 mg g−1. Moreover, the density functional theory calculations revealed an adsorption energy of −9.54 eV, indicating SA@ANDS has a good affinity for La (III). Additionally, the SA@ANDS composite hydrogel showed good adsorption capacities of 169.49, 175.43 and 178. 57 mg g−1 for Ce (III), Pr (III) and Sm (III), respectively. The comparative adsorption performance results demonstrated the SA@ANDS composite hydrogel exhibited a stronger binding affinity for La (III) compared to Al (III), Mg (II), Co (II), and Cd (II). The SA@ANDS composite hydrogel showed good recyclability for La (III) of 82.73 % after 5 cycles and was successfully applied for the extraction of La (III) from industrial wastewater.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148161"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of chitosan-derived carbon dots and Carbomer hydrogel for sustained antibacterial and immunomodulatory efficacy in treating MRSA-infected diabetic wounds. 壳聚糖衍生碳点与卡波姆水凝胶结合治疗mrsa感染的糖尿病伤口的持续抗菌和免疫调节效果

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148229

Rongjie Lin, Yao Wang, Zhenbin Chen, Shuangying Yang, Menghan Zhang, Shaohuang Weng, Jianyong Huang, Min Chen

{"title":"Integration of chitosan-derived carbon dots and Carbomer hydrogel for sustained antibacterial and immunomodulatory efficacy in treating MRSA-infected diabetic wounds.","authors":"Rongjie Lin, Yao Wang, Zhenbin Chen, Shuangying Yang, Menghan Zhang, Shaohuang Weng, Jianyong Huang, Min Chen","doi":"10.1016/j.ijbiomac.2025.148229","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148229","url":null,"abstract":"Bacterial infection and insufficient angiogenesis caused by immune dysregulation pose significant challenges to diabetic wound healing. Effectively combating bacterial infection and modulating endothelial function through the polarization of M2 macrophages to promote angiogenesis are considered as effective strategies for treating diabetic wounds. However, local treatment that achieves dual effects of antibacterial and immunomodulation through simple pathways remains limited. There is a critical need for the development of a wound dressing that possesses both antimicrobial activity and the ability to modulate the immune response within the wound microenvironment. Herein, the positively charged chitosan-derived carbon dots (CCDs) with excellent antibacterial activity were synthesized by a one-step hydrothermal method, demonstrating significant efficacy against methicillin-resistant Staphylococcus aureus (MRSA). The carbomer hydrogel (CH) was designed for loading CCDs to be the hybrid hydrogel (CCDs@CH). CCDs@CH not only possessed pH responsiveness and enhanced mechanical properties that enabled adaptation to the wound microenvironment, but also exhibited pronounced immunomodulatory and pro-angiogenic activities. In a rat model of MRSA-infected skin wound, CCDs@CH significantly accelerated wound healing, resulting in a healing rate of 72.3 % on day 7 and 98.7 % on day 14. Furthermore, a reduction in bacterial load at the wound site by over 90 % was observed in CCDs@CH group, whose cure was also confirmed by histological examination. This topical hydrogel provides a straightforward and effective approach for treating infectious wounds and offers new insights into treating diabetic wounds.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148229"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD26 acts as a host restriction factor to inhibit Influenza A virus (H1N1) infection CD26作为宿主限制因子抑制甲型流感病毒（H1N1）感染

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148150

Xiaopeng Hu , Chenyang Li , Chenshuo Shao , Haoyang Zhi , Yang Gao , Xingkui Xue

{"title":"CD26 acts as a host restriction factor to inhibit Influenza A virus (H1N1) infection","authors":"Xiaopeng Hu , Chenyang Li , Chenshuo Shao , Haoyang Zhi , Yang Gao , Xingkui Xue","doi":"10.1016/j.ijbiomac.2025.148150","DOIUrl":"10.1016/j.ijbiomac.2025.148150","url":null,"abstract":"<div><div>The frequent mutations and emerging drug resistance of Influenza A virus (IAV) pose a persistent global health challenge. The identification of host restriction factors that can inhibit viral infection is crucial for the development of novel antiviral therapies. CD26 acts as a host restriction factor in inhibiting H1N1 infection and the underlying mechanisms remain unclear. Our findings collectively demonstrate that CD26 inhibits H1N1 infection. In vitro, soluble CD26 (2.5 μg/ml) reduces H1N1 titers, specifically suppresses IL-6 upregulation in H1N1-infected A549 cells via NF-κB/IRF3/STAT1 signaling, and dose-dependently inhibits viral hemagglutinin (HA) expression. Surface Plasmon Resonance (SPR) assays confirm direct binding between sCD26 and H1N1 HA with a dissociation constant of 616 nM, while confocal microscopy demonstrates their colocalization, supporting the interaction. As compared with H1N1-infected Wild-Type mice, H1N1-infected CD26 knockout mice showed uncontrolled viral spread, more severe lung pathology, altered lung immune cell proportions, and elevated CXCL2/IL-1β/IL-6 levels in bronchoalveolar lavage fluid (BALF). Overall, this study identifies CD26 as a host restriction factor against H1N1, thereby providing a potential therapeutic avenue for managing influenza-induced lung complications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148150"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

cRGD (αvβ₃ integrin receptor)-functional thermo/pH sensitive chitosan copolymer magnetic nanoparticles for targeted therapy of breast cancer. cRGD （αvβ3整合素受体）-功能热/pH敏感壳聚糖共聚物磁性纳米颗粒靶向治疗乳腺癌。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148220

Leila Pakjo, Reza Teimuri-Mofrad, Marziyeh Fathi

{"title":"cRGD (αvβ3 integrin receptor)-functional thermo/pH sensitive chitosan copolymer magnetic nanoparticles for targeted therapy of breast cancer.","authors":"Leila Pakjo, Reza Teimuri-Mofrad, Marziyeh Fathi","doi":"10.1016/j.ijbiomac.2025.148220","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148220","url":null,"abstract":"In this study, cyclic Arg-Gly-Asp-D-Phe-Lys (cRGD)-conjugated multifunctional thermo/pH-sensitive magnetic nanoparticles (MNPs) based on chitosan (CS) were designed and synthesized for the target delivery of doxorubicin (DOX). First, CS copolymer (CS/CP) was synthesized, and then, CS/CP MNPs were prepared through a complex between the COOH groups of CS/CP and Fe3O4MNPs. Ultimately, cRGD was conjugated to the NH2 groups of the CS/CP MNPs as an integrin αvβ3 receptor-targeting ligand. The structure, size, morphology, and magnetic response of RGD/CS/CP MNPs were characterized using FT-IR, 1H NMR, and UV-Vis spectroscopies, SEM-EDX analysis, X-ray diffraction, dynamic light scattering, and a vibrating sample magnetometer. The DOX loading capacity (DLC%) and encapsulation efficiency (EE%) were about 33.26 % and 95 %, respectively, and the DOX release rate was higher at pH = 5.8. The MTT test showed that the DOX-loaded cRGD/CS/CP MNPs had significant toxicity against MCF-7 cells. The cellular uptake analysis confirmed that cRGD/CS/CP MNPs could be easily internalized by MCF-7 cells through αvβ3 integrin receptor-mediated endocytosis. The assessment of apoptosis/necrosis indicated a higher apoptosis rate of cRGD/CS/CP MNPs-DOX (80.15 %) compared to free DOX (26.99 %). Therefore, the prepared novel nanocarrier could be a good candidate as a targeted delivery agent for breast cancer treatment.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148220"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave-assisted diol DES system enables efficient biomass fractionation and lignin grafting stabilization from peanut shells via a lignin-first strategy. 微波辅助二醇DES系统通过木质素优先策略实现花生壳的高效生物质分馏和木质素接枝稳定。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148154

Tianle Xie, Li Chen, Manni Ren, Qinghua Ji, Cunshan Zhou

{"title":"Microwave-assisted diol DES system enables efficient biomass fractionation and lignin grafting stabilization from peanut shells via a lignin-first strategy.","authors":"Tianle Xie, Li Chen, Manni Ren, Qinghua Ji, Cunshan Zhou","doi":"10.1016/j.ijbiomac.2025.148154","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148154","url":null,"abstract":"Lignocellulosic biomass represents a promising renewable resource, but its valorization is often hindered by irreversible lignin condensation during conventional pretreatment processes. This study developed a novel microwave-assisted diol-based deep eutectic solvent (DES) system (ChCl/diol/AlCl₃·6H₂O) to achieve integrated fractionation of peanut shells while preserving the lignin structure. Under mild conditions (120 °C, 30 min), the system enabled near-complete removal of hemicellulose (91.17 %) and lignin (86.39 %), yielding cellulose-rich solids with enhanced enzymatic digestibility (glucose: 92.64 %, xylose: 94.56 %). Importantly, diol achieved α-etherification grafting of lignin, thereby inhibiting lignin condensation. The grafted lignin retained an intact structure with a high β-O-4 content (39.29 per 100 Ar) and retention rate (84.19 %). The DES exhibited excellent recyclability and 73.04 % total biomass valorization. This work provides a sustainable approach for integrated lignocellulose biorefining.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148154"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic polypyrrole-alginate microspheres derived from biogenic CaCO₃ from oyster shells for bisphenol A removal in aqueous environment: Kinetics and thermodynamic study. 磁性聚吡啶-海藻酸微球从牡蛎壳中提取生物CaCO3去除水环境中的双酚A：动力学和热力学研究。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148192

Sunday Ikechukwu Onukwube, Si Ling Ng, Musfirah Zulkurnain, Azim Patar, Noorfatimah Yahaya, Ngee Sing Chong, Yong Foo Wong

{"title":"Magnetic polypyrrole-alginate microspheres derived from biogenic CaCO3 from oyster shells for bisphenol A removal in aqueous environment: Kinetics and thermodynamic study.","authors":"Sunday Ikechukwu Onukwube, Si Ling Ng, Musfirah Zulkurnain, Azim Patar, Noorfatimah Yahaya, Ngee Sing Chong, Yong Foo Wong","doi":"10.1016/j.ijbiomac.2025.148192","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148192","url":null,"abstract":"Adsorption is a widely used remediation technique for the removal of bisphenol A (BPA) in water, yet many adsorbents exhibit limited adsorption capacity and poor reusability. To address these limitations, this study synthesized a low-cost and green porous magnetic polypyrrole@Fe3O4@alginate adsorbent (magnetic strength of 2.658 emu/g), utilizing sacrificial CaCO3 derived from waste oyster shells. The magnetic adsorbent was comprehensively characterized using FTIR, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, vibrating sample magnetometry, thermogravimetric analysis, and Brunauer-Emmett-Teller analysis, confirming its structural and morphological integrity. Adsorption performance was investigated using spectrofluorimetric analysis under optimized conditions (5 mg/L BPA, pH 3, 25 °C, 50 min contact time, and 20 mg adsorbent dosage), provided a maximum adsorption capacity of 87.23 mg/g at 25 °C. Kinetic studies demonstrated that the adsorption process followed Elovich kinetic model with R2 = 0.9993 and the lowest χ2 = 2.6746, while equilibrium data were best described by the Redlich-Peterson isotherm model with the highest R2 = 0.9977 and the lowest χ2 = 2.6746. Notably, the adsorbent demonstrated good adsorption efficiency, and relatively good reusability (removal efficiency of 41.5 % efficiency after five cycles), highlighting its potential for removing BPA removal from aqueous environments.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148192"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

p  p <通过连接体工程提高里氏木霉纤维生物水解酶II （CBHII）的水解活性和木质素抗性。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148218

Yuxuan Tian, Meng Zhang, Liangkun Long, Shaojun Ding

{"title":"p <Improving the hydrolytic activity and lignin resistance of cellobiohydrolase II (CBHII) from Trichoderma reesei through linker engineering.","authors":"Yuxuan Tian, Meng Zhang, Liangkun Long, Shaojun Ding","doi":"10.1016/j.ijbiomac.2025.148218","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148218","url":null,"abstract":"Cellobiohydrolase II (CBHII) is the second most abundant enzyme in cellulase cocktails derived from Trichoderma reesei. Compared to CBHI, the linker region of CBHII is longer and contains more arginine residues. However, the role of these features in regulating CBHII catalytic activity and lignin tolerance remains poorly understood. In this study, linker-engineered CBHII variants were created by gradually shortening the central part or inserting or substituting arginine residues in linker region, aiming to improve hydrolytic activity and lignin resistance. The presence of the first arginine residue near CBM1 was essential for the proper folding and expression of CBHII. Reducing the length of the linker, as well as decreasing the number of arginine residues and O-glycosylation sites, significantly influenced the catalytic properties, cellulose and lignin-binding capabilities, and solution-phase enzyme conformation. Two engineered variants, CBHII-L-14 and CBHII-L-17, showed notable enhancements in both catalytic performance and lignin resistance, achieving 67.7 % and 60.5 % greater hydrolysis efficiency, respectively, compared with the wild-type CBHII enzyme when acting on filter paper (FP) in lignin-rich environments. Small-angle X-ray scattering (SAXS) experiments indicated these engineered variants possessed shortened yet structurally more rigid linker regions. The combination effect of the more rigidity of the shorter linker, along with the reduced arginine residues and O-glycosylation sites likely accounts for their improved catalytic activities and reduced lignin inhibition. The findings suggest that engineering shorter linker regions in CBHII represents a viable approach for increasing catalytic efficiency, thereby offering potential advancements in enzyme robustness and enhanced hydrolysis of lignocellulosic biomass.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148218"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azobenzene-integrated photoresponsive DNA with universal design principles and cross-disciplinary applications: A review 偶氮苯集成光反应DNA的通用设计原理及其跨学科应用综述。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148159

Chenxi Li , Mengqiu Sun , Rui Song , Zhenshuai Tang , Hao Zhang

{"title":"Azobenzene-integrated photoresponsive DNA with universal design principles and cross-disciplinary applications: A review","authors":"Chenxi Li , Mengqiu Sun , Rui Song , Zhenshuai Tang , Hao Zhang","doi":"10.1016/j.ijbiomac.2025.148159","DOIUrl":"10.1016/j.ijbiomac.2025.148159","url":null,"abstract":"<div><div>Photoresponsive DNA nanotechnology represents an important platform for creating dynamic biomaterials and therapeutic tools. This review presents a systematic approach linking molecular design principles with functional outcomes in azobenzene-DNA systems. Moving beyond previous summaries, we examine key structural features including nucleobase modifications, backbone architectures, and supramolecular organization that govern performance on biological and materials applications. These structural insights explain observed variations in system performance and guide rational design strategies. Recent experimental advances demonstrate practical applications of these principles, particularly in developing infrared-sensitive switches and predictive modeling approaches. The established correlations between molecular structure and functional output provide actionable guidelines for creating more effective light-responsive materials. By bridging fundamental photochemical principles with engineering considerations, this framework facilitates the development of reliable DNA-based systems for biomedical research and nanotechnology applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"330 ","pages":"Article 148159"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intrinsically disordered enteropathogenic E. coli EspF exploits motif mimicry in high-affinity binding to neural Wiskott-Aldrich syndrome protein and sorting nexin 9. 内在紊乱的肠致病性大肠杆菌EspF利用基序模仿与神经Wiskott-Aldrich综合征蛋白和分类连接蛋白9的高亲和力结合。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148227

Helena Tossavainen, Mikael Karjalainen, Lina Antenucci, Maarit Hellman, Perttu Permi

{"title":"Intrinsically disordered enteropathogenic E. coli EspF exploits motif mimicry in high-affinity binding to neural Wiskott-Aldrich syndrome protein and sorting nexin 9.","authors":"Helena Tossavainen, Mikael Karjalainen, Lina Antenucci, Maarit Hellman, Perttu Permi","doi":"10.1016/j.ijbiomac.2025.148227","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148227","url":null,"abstract":"EspF is an enteropathogenic Escherichia coli (EPEC) effector protein that interferes with intestinal epithelial cell signaling by binding to the Src homology 3 (SH3) domain of sorting nexin 9 (SNX9) and the GTPase-binding domain (GBD) of neural Wiskott-Aldrich syndrome protein (N-WASP) with its C-terminal proline-rich repeats. To understand the molecular basis of these interactions, we characterized the structure, dynamics, and binding thermodynamics of EspF and its target protein domain complexes. We also elaborated on our previous study on EspFU, a homologous effector in enterohemorrhagic E. coli (EHEC), and compared the two effectors. We show that EspF is intrinsically disordered but that NMR chemical shifts expose the pre-structured polyproline II (PPII) helical SH3- and helical GBD-binding motifs. These motifs mimic their cellular counterparts but are fine-tuned to prevail in competitive binding. Factors behind EspF's higher affinity for GBD relative to the cellular ligand are key residue mutations and a C-terminally elongated polar interaction interface. The latter compensates for the lack of an \"extended arm\", the critical substitution promoting high affinity for GBD in EspFU. With this advantage, EspF outcompetes the autoinhibitory N-WASP C-helix and stimulates actin polymerization. EspF binds SNX9 SH3 with an extended binding interface, residues N-terminal to the RxAPxxP core motif being essential to strong binding. We define the SNX9 SH3-binding epitope as ϕxPxRxAPxxP and propose to re-delineate the EPEC EspF repeat boundaries accordingly. Furthermore, a characteristic 13C secondary chemical shift pattern is recognized as a fingerprint of polyproline II (PPII) helical conformation in the SH3 binding epitope.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148227"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative bioinformatics and experimental validation identify pyrroline-5-carboxylate reductase 1 as a mitochondrial regulator and diagnostic biomarker in osteosarcoma. 综合生物信息学和实验验证鉴定吡咯-5-羧酸还原酶1是骨肉瘤的线粒体调节因子和诊断生物标志物。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI: 10.1016/j.ijbiomac.2025.148083

Pan Jang, Chunshan Luo, Mingjiu Chen, Yangyang Peng, Tingsheng Lu, Xuan Wang, Yicheng Yang, Xingwei Pu, Shuo Lu

{"title":"Integrative bioinformatics and experimental validation identify pyrroline-5-carboxylate reductase 1 as a mitochondrial regulator and diagnostic biomarker in osteosarcoma.","authors":"Pan Jang, Chunshan Luo, Mingjiu Chen, Yangyang Peng, Tingsheng Lu, Xuan Wang, Yicheng Yang, Xingwei Pu, Shuo Lu","doi":"10.1016/j.ijbiomac.2025.148083","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.148083","url":null,"abstract":"Osteosarcoma (OS) is a highly aggressive bone malignancy with limited effective biomarkers and therapeutic strategies. An integrative analysis was conducted combining transcriptomic profiling, mitochondrial-related gene (MRG) screening, and machine learning approaches to uncover novel diagnostic and prognostic markers. Among differentially expressed MRGs, enrichment analyses highlighted key metabolic pathways. Machine learning models consistently identified pyrroline 5-carboxylate reductase 1 (PYCR1) as the most robust biomarker, achieving high diagnostic accuracy across independent cohorts. Elevated PYCR1 expression was significantly associated with poor prognosis. Functional experiments demonstrated that PYCR1 knockdown suppressed OS cell proliferation, migration, and invasion, while inducing mitochondrial structural disruption, increased ROS, and altered membrane potential, underscoring its essential role in mitochondrial homeostasis and tumor aggressiveness. Moreover, immune infiltration analysis revealed PYCR1-driven remodeling of the tumor immune microenvironment. Collectively, our findings establish PYCR1 as a mitochondrial regulator integrating metabolic and immunological mechanisms in OS and highlight its potential as a biomarker and therapeutic target for precision diagnosis and mitochondria-targeted interventions.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"148083"},"PeriodicalIF":8.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0