International Journal of Biological Macromolecules最新文献_第5页

Tailoring of amino functionalized graphene oxide/cellulose/chitosan composite for the determination of imidacloprid in milk samples. 氨基功能化氧化石墨烯/纤维素/壳聚糖复合材料测定牛奶样品中的吡虫啉。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-04 DOI: 10.1016/j.ijbiomac.2026.152385

Waleed Altaf, Nadeem Raza, Muhammad Hayat, Saba Khalid, Farah Khan, Faisal K Algethami, Jameel Ahmad Baig, Mohamed Rahmtalla Elamin, Suryyia Manzoor

{"title":"Tailoring of amino functionalized graphene oxide/cellulose/chitosan composite for the determination of imidacloprid in milk samples.","authors":"Waleed Altaf, Nadeem Raza, Muhammad Hayat, Saba Khalid, Farah Khan, Faisal K Algethami, Jameel Ahmad Baig, Mohamed Rahmtalla Elamin, Suryyia Manzoor","doi":"10.1016/j.ijbiomac.2026.152385","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.152385","url":null,"abstract":"<p><p>Cellulose and chitosan, due to their high mechanical strength and active functional groups are preferred in designing novel composites for solid phase extraction of diverse analytes from food, biological or environmental samples. In this study, an amino functionalized graphene oxide, cellulose and chitosan-based composite was designed and well characterized for the analysis of imidacloprid (IMI) in cow milk samples. Characterization of synthesized composite was done by using Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray, thermogravimetric analysis and differential scanning calorimetry. Limit of detection and quantification of pesticide were found to be 5.5 × 10<sup>-3</sup> mgL<sup>-1</sup> and 1.65 × 10<sup>-2</sup> mgL<sup>-1</sup>. Kinetic studies revealed that the adsorption process was governed by the pseudo first order mechanism. Optimization of different parameters revealed that best conditions for IMI adsorption were pH 5, adsorbent dose of 20 mg, IMI concentration of 35 mgL<sup>-1</sup> and the contact time of 20 min. Thermodynamic studies showed ΔH° value of +20.0 kJmol<sup>-1</sup>, ΔS<sup>°</sup>, +155.1 JK<sup>-1</sup> mol<sup>-1</sup> and ΔG<sup>°</sup> at 298 K, 308 K, 318 K was -26.13, -27.68 and - 29.23 kJmol<sup>-1</sup>, respectively. To evaluate the real-time application of designed composite, IMI was determined in cow milk samples using dispersive solid phase microextraction followed by high-performance liquid chromatography. The composite showed a high percentage recovery of 95.4 ± 4.1% (n = 9) of IMI. These results indicate the effectiveness of composite in detecting the IMI in complex matrixes like milk, thus highlighting its effectiveness and potential as a reliable method in food analysis.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"152385"},"PeriodicalIF":8.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structurally engineered lignin-based robust, conductive ionic hydrogels via DMSO-assisted dual-network strategy for flexible sensors. 结构工程木质素为基础的坚固，导电离子水凝胶通过dmso辅助双网络策略柔性传感器。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-04 DOI: 10.1016/j.ijbiomac.2026.152384

Xiaoqian Zhou, Jiatong Zhou, Yuanyuan Xu, Linlin Zhao, Cunshi Zhao, Ailing Xu, Xinhui Wang, Tianpeng Zhang, Dong Wang, Qingbo Wang, Yonggui Wang

{"title":"Structurally engineered lignin-based robust, conductive ionic hydrogels via DMSO-assisted dual-network strategy for flexible sensors.","authors":"Xiaoqian Zhou, Jiatong Zhou, Yuanyuan Xu, Linlin Zhao, Cunshi Zhao, Ailing Xu, Xinhui Wang, Tianpeng Zhang, Dong Wang, Qingbo Wang, Yonggui Wang","doi":"10.1016/j.ijbiomac.2026.152384","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.152384","url":null,"abstract":"<p><p>Electronic skins and wearable sensors exhibit enormous application potential in emerging fields such as human health monitoring and human-machine interaction. Ionic hydrogels are promising soft conductors for electronic skins and wearable sensors, but they suffer from weak mechanical performance, swelling, and unstable ionic conduction in practical scenarios. Here, we propose a DMSO-assisted dual-network strategy for constructing lignin-based ionic hydrogels that integrate robustness, high ionic conductivity, and antibacterial activity. Alkaline lignin is modified into a multifunctional building block (LigBM<sub>3</sub>-C<sub>16</sub>) with crosslinkable sites and antibacterial property via esterification and quaternization. Copolymerization of LigBM<sub>3</sub>-C<sub>16</sub> with acrylic acid and acrylamide in DMSO forms a homogeneous covalent DMSO gel, followed by FeCl<sub>3</sub> solvent exchange to capture Fe<sup>3+</sup> on uniformly phenolic and carboxylic groups from lignin and acrylic acid, forming a continuous hydrate coordination network. The resulting LigBM<sub>3</sub>-C<sub>16</sub>-Fe hydrogels exhibits excellent tensile strength (3.38 MPa) and toughness (9.76 MJ m<sup>-3</sup>) and ionic conductivity (σ = 27.4 mS cm<sup>-1</sup>), overcoming the typical trade-off between robustness and ion transport. Fe<sup>3+</sup> coordination and quaternized lignin together provide broad-spectrum antibacterial/antioxidant activity. The resulting ionic hydrogel functions as stable strain sensors for real-time human motion monitoring and gesture-controlled human-machine interaction, offering a simple method to robust, multifunctional ionic hydrogels for flexible conducting materials.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"152384"},"PeriodicalIF":8.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of tea processing wastewater into multifunctional chitosan-based films for active food packaging. 茶叶加工废水转化为食品活性包装用多功能壳聚糖基薄膜的研究。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-03 DOI: 10.1016/j.ijbiomac.2026.152368

Tianyi Hu, Wanqin Xu, Qun Liu, Nan Zhou, Wuyin Weng, Yucang Zhang

引用次数: 0

Angelica sinensis polysaccharides modulate iron metabolism and macrophage polarization to alleviate rheumatoid arthritis 当归多糖调节铁代谢和巨噬细胞极化减轻类风湿关节炎。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-01 Epub Date: 2026-04-23 DOI: 10.1016/j.ijbiomac.2026.152182

Yuxue Zhan , Hengqi Zhang , Tingting Guo , Hailong Qin , Lei Huang , Nire Wu , Jingyi Wang , Xu Mu , Jinglin Wang , Mingming Li , Kaiping Wang , Yu Zhang

{"title":"Angelica sinensis polysaccharides modulate iron metabolism and macrophage polarization to alleviate rheumatoid arthritis","authors":"Yuxue Zhan , Hengqi Zhang , Tingting Guo , Hailong Qin , Lei Huang , Nire Wu , Jingyi Wang , Xu Mu , Jinglin Wang , Mingming Li , Kaiping Wang , Yu Zhang","doi":"10.1016/j.ijbiomac.2026.152182","DOIUrl":"10.1016/j.ijbiomac.2026.152182","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation and systemic complications. Recent evidence has indicated that disrupted iron metabolism, notably elevated hepcidin expression and excessive iron accumulation in tissues, contributes to RA progression. In RA, Macrophages, which play a central role in both immune regulation and iron homeostasis, are often polarized toward the proinflammatory M1 phenotype. In this study, we investigated the therapeutic effects of <em>Angelica sinensis</em> polysaccharides (ASP, 100–400 mg/kg) in a collagen-induced arthritis (CIA) mouse model. ASP significantly alleviated joint swelling, histopathological damage, and iron deposition in the liver and spleen. It also downregulated hepcidin expression and restored the M1/M2 macrophage balance. Furthermore, immunofluorescence and western blotting confirmed increased ferroportin (FPN) and decreased ferritin levels in splenic macrophages. In an <em>in vitro</em> coculture model mimicking inflammation, ASP indirectly modulated macrophage iron handling and polarization by suppressing hepatocyte-derived hepcidin. RNA-seq analysis revealed that ASP treatment downregulated HIF-1α and NF-κB pathways, which link iron metabolism to macrophage activation, whereas the results of reactive oxygen species (ROS) assays supported its role in reducing oxidative stress associated with iron overload. Together, these findings suggest that ASP alleviates RA symptoms by restoring iron homeostasis and modulating macrophage polarization, primarily by targeting the hepcidin-FPN axis and related inflammatory pathways. ASP therefore holds promise as a low-toxicity, immunomodulatory candidate for RA therapy.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"364 ","pages":"Article 152182"},"PeriodicalIF":8.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, characterization, and pre-clinical evaluation of acarbose-guar gum solid dispersions in diabetic rats 糖尿病大鼠糖瓜尔胶固体分散体的制备、表征和临床前评价。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-01 Epub Date: 2026-04-22 DOI: 10.1016/j.ijbiomac.2026.152172

Sourbh Suren Garg , Ankit Saneja , Jeena Gupta

{"title":"Preparation, characterization, and pre-clinical evaluation of acarbose-guar gum solid dispersions in diabetic rats","authors":"Sourbh Suren Garg , Ankit Saneja , Jeena Gupta","doi":"10.1016/j.ijbiomac.2026.152172","DOIUrl":"10.1016/j.ijbiomac.2026.152172","url":null,"abstract":"<div><div>Acarbose, a clinically approved α-glucosidase inhibitor for type 2 diabetes, has limited efficacy due to gastrointestinal side effects, highlighting the need for delivery systems that modulate release and improve tolerability. This study aimed to develop and characterize acarbose-guar gum solid dispersions (AGSDs) and evaluate their physicochemical, <em>in vitro</em>, and <em>in vivo</em> properties. Solid-state characterization confirmed amorphous molecular dispersion with spherical particles and drug-polymer interactions. Among the developed solid dispersions, AGSD-3 showed the highest drug entrapment efficiency (84.24 ± 0.48%) and controlled release (86.19 ± 1.66% over 24 h), compared to free acarbose. It exhibited strong enzyme inhibition with IC₅₀ values of 6.97 ± 0.21 μg/mL (α-glucosidase) and 12.86 ± 0.18 μg/mL (α-amylase), along with favourable cytocompatibility and hemocompatibility. In high-fat diet (HFD)-fed, streptozotocin (STZ)-induced diabetic rat model, AGSD-3 at 50 mg/kg significantly reduced body weight and blood glucose (<em>p</em> < 0.001), while gastrointestinal side effects, such as diarrhoea, were limited to a single day (score 1), indicating improved tolerability compared to acarbose alone. Treatment also restored pancreatic weight (<em>p</em> < 0.001) and histology, and attenuated oxidative stress, and inflammation (p < 0.001), indicating protective effects against diabetes-induced tissue damage. Overall, these findings suggest that guar gum-based solid dispersion represents a promising strategy to enhance the therapeutic efficacy and tolerability of acarbose for diabetes management.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"364 ","pages":"Article 152172"},"PeriodicalIF":8.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-tunable electro-responsive sodium alginate/chitosan-based Janus polyelectrolyte hydrogel with reversible bidirectional bending ph可调电响应海藻酸钠/壳聚糖基Janus聚电解质可逆双向弯曲水凝胶。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-01 Epub Date: 2026-04-27 DOI: 10.1016/j.ijbiomac.2026.152237

Ting Wu , Qiwei Wang , Sihan Zhou , Hecheng Chen , Libing Zhang

{"title":"pH-tunable electro-responsive sodium alginate/chitosan-based Janus polyelectrolyte hydrogel with reversible bidirectional bending","authors":"Ting Wu , Qiwei Wang , Sihan Zhou , Hecheng Chen , Libing Zhang","doi":"10.1016/j.ijbiomac.2026.152237","DOIUrl":"10.1016/j.ijbiomac.2026.152237","url":null,"abstract":"<div><div>Polyelectrolyte hydrogels are ideal for soft actuators, but conventional chitosan/sodium alginate (CS/SA) ionic hydrogels face a critical trade-off among flexibility, ionic content, and actuation speed, hindering their practical applications. Herein, dimethylaminoethyl methacrylate (DMAEMA) and sodium acrylate (AAS) were used to modify CS-based polycationic and SA-based polyanionic networks, respectively. A Janus polyelectrolyte hydrogel (PCDM/PSSM) with seamless interface was fabricated via one-pot synthesis, electrohydrodynamic (EHD) printing, and in-situ photopolymerization. Microstructural characterization verified successful monomer grafting, amorphous/low-crystallinity networks, and robust interlocked interfaces. The hydrogel exhibited superior mechanical properties (PSSM achieving a tensile stress of 78.6 kPa and a strain of 775.6%, and PCDM achieving a tensile stress of 52.2 kPa and a strain of 808.9%, respectively), high ionic conductivity (13.81 × 10<sup>−3</sup> S/cm for PSSM), and efficient electro-actuation (155° bending at 10 V). Its core advantage is pH-programmable reversible bidirectional bending: −145° toward the polyanionic layer at pH = 2 and + 151° toward the polycationic layer at pH ≥ 7, originating from pH-regulated asymmetric swelling of protonated/deprotonated functional groups and enhanced by electric-field-induced ion migration. Demonstrated applications included an underwater soft gripper for fragile object manipulation and a biomimetic flower for pH-responsive petal closure. This work resolves the longstanding trade-off of CS/SA hydrogels and provides a scalable strategy for high-performance stimuli-responsive soft actuators, promising broad use in soft robotics, bioinspired systems, and biomedical engineering.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"363 ","pages":"Article 152237"},"PeriodicalIF":8.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phage display enabled the identification of single-domain nanobody antitoxins against botulinum neurotoxin serotype C 通过噬菌体展示，鉴定出了针对C型肉毒杆菌神经毒素的单域纳米体抗毒素。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-01 Epub Date: 2026-04-24 DOI: 10.1016/j.ijbiomac.2026.152226

Ziye Liu , Qinqin Wang , Xinrui Yue , Jinghan He , Xiaoru Wang , Fuwei Qi , Wantong Ma , Bo Liu , Dang Ding , Xinyao Ma , Lunjia Zhang , Xin Wang , Dejuan Zhi , Dongsheng Wang

{"title":"Phage display enabled the identification of single-domain nanobody antitoxins against botulinum neurotoxin serotype C","authors":"Ziye Liu , Qinqin Wang , Xinrui Yue , Jinghan He , Xiaoru Wang , Fuwei Qi , Wantong Ma , Bo Liu , Dang Ding , Xinyao Ma , Lunjia Zhang , Xin Wang , Dejuan Zhi , Dongsheng Wang","doi":"10.1016/j.ijbiomac.2026.152226","DOIUrl":"10.1016/j.ijbiomac.2026.152226","url":null,"abstract":"<div><div>Botulinum neurotoxin serotype C (BoNT/C) exhibits therapeutic efficacy and duration of action comparable to those of BoNT/A. It has been demonstrated to effectively manage movement disorders such as dystonia and blepharospasm, making it a highly promising alternative to BoNT/A. BoNT/C is also one of the primary etiological botulinum isoforms causing animal botulism and may pose a zoonotic risk. So, it is of great need to develop antidote that can treat human iatrogenic, foodborne and animal botulism caused by BoNT/C. Currently, the approved botulism antidotes primarily are polyvalent horse serum antitoxins, which are severely limited due to insufficient supply and adverse reactions. In this study, we developed a high-affinity nanobody A2, a variable domain of a heavy-chain antibody (VHH) against BoNT/C, based on phage display library screening. Our results demonstrated that A2 effectively blocked the binding of BoNT/C to its target cell. Furthermore, A2 exhibited potent neutralizing activity and rescued BoNT/C-intoxicated mice. These findings supported nanobody A2 as a promising antitoxin candidate with therapeutic efficacy against botulism.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"364 ","pages":"Article 152226"},"PeriodicalIF":8.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitellogenin and vitellogenin receptor in crustaceans: Macromolecular design rules linking yolk deposition, oocyte quality, and innate immunity 甲壳类动物的卵黄原蛋白和卵黄原蛋白受体：连接卵黄沉积、卵母细胞质量和先天免疫的大分子设计规则。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-01 Epub Date: 2026-04-27 DOI: 10.1016/j.ijbiomac.2026.152242

Enjie Chua , Yin Zhang , Leong-Seng Lim , Xueyu Yan , Kianann Tan

{"title":"Vitellogenin and vitellogenin receptor in crustaceans: Macromolecular design rules linking yolk deposition, oocyte quality, and innate immunity","authors":"Enjie Chua , Yin Zhang , Leong-Seng Lim , Xueyu Yan , Kianann Tan","doi":"10.1016/j.ijbiomac.2026.152242","DOIUrl":"10.1016/j.ijbiomac.2026.152242","url":null,"abstract":"<div><div>Vitellogenin (Vg) and the vitellogenin receptor (VgR) are usually discussed as endocrine readouts of ovarian maturation. Here they are treated as a coupled macromolecular system: a high-mass lipid/glyco-protein that must remain stable in hemolymph and a modular endocytic receptor that governs selective uptake, intracellular routing, and storage conversion. This perspective brings several underused mechanistic layers into focus. First, Vg performance is likely encoded by proteoforms created by glycosylation, proteolytic processing, and cargo loading, as shown by site-specific high-mannose glycans in crayfish Vg. Second, VgR functions as a kinetic throughput valve whose binding and recycling properties can become rate-limiting for yolk deposition, supported by receptor biochemistry in mud crab and functional silencing in shrimp. Third, immune functions and reproduction are directly coupled which Vg not only binds microbes via defined domains but also provides systemic antiviral and antioxidant defense, while ovarian VgR expression can modulate systemic immunity by regulating hemolymph Vg accumulation. Finally, recent evidence indicates that VgR-mediated uptake is a stress-buffering mechanism that protects vitellogenic oocyte formation under broader environmental challenges, including thermal, salinity, and heavy metal stress. We synthesize these findings into testable design rules and propose a practical toolkit such as glycoproteomics, lipidomics, binding biophysics, and structure-guided functional assays to move the field from transcript-level descriptions to predictive, intervention-ready mechanisms for elite aquaculture breeding and farm management.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"363 ","pages":"Article 152242"},"PeriodicalIF":8.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization and antitumor activity of a polysaccharide from Polygonatum cyrtonema Hua 黄精多糖的结构表征及抗肿瘤活性。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-01 Epub Date: 2026-04-27 DOI: 10.1016/j.ijbiomac.2026.152253

Leilei Gao , Lu Song , Junhao Ji , Susu Yang , Fang Wang , Jinfeng Chen , Dong Liu , Wei Wang , Bangxing Han

{"title":"Structural characterization and antitumor activity of a polysaccharide from Polygonatum cyrtonema Hua","authors":"Leilei Gao , Lu Song , Junhao Ji , Susu Yang , Fang Wang , Jinfeng Chen , Dong Liu , Wei Wang , Bangxing Han","doi":"10.1016/j.ijbiomac.2026.152253","DOIUrl":"10.1016/j.ijbiomac.2026.152253","url":null,"abstract":"<div><div>Although polysaccharides from <em>Polygonatum cyrtonema</em> Hua have long been considered promising bioactive components, their antitumor effects have remained insufficiently defined. In this study, a homogeneous polysaccharide, PHP-1, was isolated and purified from <em>Polygonatum cyrtonema</em> Hua. Its structural characteristics and anti-tumor activity were analyzed. Structural characterization showed that PHP-1 was a branched fructan with a relatively narrow molecular weight distribution. In vitro experiments revealed that PHP-1 significantly inhibited the proliferation of several tumor cell lines, with the most pronounced effect observed in A375 cells. Additionally, in vitro experiments demonstrated that PHP-1 induced cell cycle arrest and apoptosis by modulating the expression of key regulatory proteins, including CDK2, Cyclin A2, Bax, and Bcl-2. In vivo studies further confirmed that PHP-1 promotes apoptosis and effectively inhibits tumor growth in tumor-bearing mouse model. This study provides preliminary insights into the potential role of the newly identified homogeneous polysaccharide PHP-1 in cancer therapy, offering a theoretical foundation for the high-value utilization of <em>Polygonatum cyrtonema</em> Hua in anticancer research and related functional products.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"364 ","pages":"Article 152253"},"PeriodicalIF":8.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of gellan gum-based bilayer films by incorporating tea polyphenols and Artemisia sphaerocephala Krasch. gum 茶多酚和黄花蒿对结冷胶基双层膜的增强作用。口香糖。

IF 8.5 1区化学

International Journal of Biological Macromolecules Pub Date : 2026-05-01 Epub Date: 2026-04-23 DOI: 10.1016/j.ijbiomac.2026.152213

Yuxuan Peng , Xu Zhang , Chunwei Li

{"title":"Enhancement of gellan gum-based bilayer films by incorporating tea polyphenols and Artemisia sphaerocephala Krasch. gum","authors":"Yuxuan Peng , Xu Zhang , Chunwei Li","doi":"10.1016/j.ijbiomac.2026.152213","DOIUrl":"10.1016/j.ijbiomac.2026.152213","url":null,"abstract":"<div><div>Natural polymers are increasingly recognized as sustainable alternatives to petroleum-based plastics, supporting cradle-to-cradle packaging strategies. Here, a pH-responsive bilayer film (GAC) was developed fabricated with an inner layer based on gellan gum (GG) blended with <em>Artemisia sphaerocephala</em> Krasch. gum (ASKG) and modified mulberry anthocyanins, and an outer layer based on cellulose acetate containing geranyl acetate. A bilayer film without ASKG served as the control (GC). The effects of incorporating tea polyphenols (TP) at different concentrations into the inner-layer matrix were further evaluated. Incorporation of ASKG and TP significantly improved mechanical performance, water resistance, antioxidant capacity, and antibacterial activity. The highest tensile strength (23.46 MPa) was achieved at a TP concentration of 2 g/L (GAC-TP2). In addition, GAC-TP2 exhibited the lowest water vapor permeability (4.78 × 10<sup>−8</sup> g∙m<sup>−1</sup>∙s<sup>−1</sup>∙Pa<sup>−1</sup>), and the water contact angle increased from 40.85° to 54.3°. Antioxidant activity also increased, with DPPH and ABTS radical-scavenging rates rising from 75.62% to 91.61% and from 92.97% to 99.49%, respectively. Overall, GAC-TP2 showed balanced, superior performance, indicating strong potential for intelligent and active food-packaging applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"364 ","pages":"Article 152213"},"PeriodicalIF":8.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0