Bionanoclusters of chitosan, indole acetic acid and cobalt chloride enhance seed germination in upland cotton by modulating glyoxylate cycle enzymes and biochemical responses

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI:10.1016/j.ijbiomac.2025.144735

Vikram Singh , Shiwani Mandhania , Rashi Datten , Ajay Pal , Vinod Saharan , Om Prakash Ghimire

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引用次数: 0

Abstract

Seed priming is a quick and easy technique to boost seed germination, seedling development and plant growth. Among many exogenous agents, nanoparticles are one of the promising agents which enable the controlled delivery of multiple active agents simultaneously, improving efficiency and effectiveness. In the present investigation, bionanoclusters (BNCs) composed of cobalt (Co), indole-3-acetic acid (IAA), and chitosan were synthesized and employed as seed priming agents in cotton seeds to examine their effects on seed germination as well as associated phenotypic and biochemical changes. These BNCs demonstrated monodispersity, stability, and a slow release of Co and IAA. Seed priming with 100 mg/l BNCs significantly increased the germination by 16.33 % (p < 0.01). Compared to the control, seedlings from BNCs-primed seeds exhibited significantly longer roots (54.27 %) and greater biomass (28.96 %) by the 12th day of germination (p < 0.01). Additionally, BNCs priming increased the activities of key enzymes of the glyoxylate cycle, including isocitrate lyase (20.43 %) and malate synthase (29.17 %), as well as total soluble sugar content (42.27 %) on the 3rd day of germination to support seed germination. Results highlight the promising role of BNCs in improving seed germination to improve crop productivity.

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壳聚糖、吲哚乙酸和氯化钴的生物纳米团簇通过调节glyoxylate cycle酶和生化反应来促进陆地棉花种子萌发

种子催熟是一种快速简便的促进种子萌发、幼苗发育和植物生长的技术。在众多外源性药物中，纳米颗粒是一种很有前途的药物，它可以同时控制多种药物的递送，提高效率和效果。本研究合成了由钴（Co）、吲哚-3-乙酸（IAA）和壳聚糖组成的生物纳米簇（bnc），并将其作为棉花种子激发剂，研究了它们对棉花种子萌发的影响以及相关的表型和生化变化。这些bnc表现出单分散性、稳定性和Co和IAA的缓慢释放。100 mg/l bnc灌种使种子萌发率显著提高了16.33% (p <；0.01)。与对照相比，经bnc处理的种子在萌发后第12天根系显著延长（54.27%），生物量显著增加（28.96%）。0.01)。此外，在萌发第3天，BNCs启动提高了异柠檬酸裂解酶（20.43%）和苹果酸合成酶（29.17%）等glyoxylate循环关键酶的活性，以及总可溶性糖含量（42.27%），支持种子萌发。结果表明，bnc在促进种子萌发、提高作物产量方面具有重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.