International Journal of Biological Macromolecules最新文献_第2页

Methacrylated-κ-carrageenan/hydroxyapatite composite bioinks for extrusion-based bioprinting: Physicochemical, rheological, mechanical, and in vitro biological investigations 用于挤压生物打印的甲基丙烯酸-κ-卡拉胶/羟基磷灰石复合生物墨水：物理化学、流变学、力学和体外生物学研究

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-28 DOI: 10.1016/j.ijbiomac.2025.144745

Seyithan Kansız , Murat Taner Vurat , Mahmut Parmaksiz , Ayşe Eser Elçin , Yaşar Murat Elçin

{"title":"Methacrylated-κ-carrageenan/hydroxyapatite composite bioinks for extrusion-based bioprinting: Physicochemical, rheological, mechanical, and in vitro biological investigations","authors":"Seyithan Kansız , Murat Taner Vurat , Mahmut Parmaksiz , Ayşe Eser Elçin , Yaşar Murat Elçin","doi":"10.1016/j.ijbiomac.2025.144745","DOIUrl":"10.1016/j.ijbiomac.2025.144745","url":null,"abstract":"<div><div>Composite hydrogels are of interest in modulating the rheological properties of bioinks for extrusion-based 3D-bioprinting. In this study, new composite bioinks composed of different levels of methacrylated κ-carrageenan (κ-Car-L-MA, κ-Car-M-MA, and κ-Car-H-MA) and hydroxyapatite (HAp) were prepared and evaluated for extrusion-based 3D-bioprinting applications, focusing on printability, mechanical and physicochemical properties. The methacrylation degree and incorporation of HAp were found to significantly affect the printing performance of bioinks. Semi-quantitative printability assessment revealed that κ-Car-M-MA and κ-Car-M-MA-HAp bioinks exhibited optimal printability. The mechanical behavior of the bioinks appeared to depend on the methacrylation degree, which affects compressive modulus and toughness. Bioprinting studies were conducted to create a bilayer model using human adipose-derived stem cells and an almost two-fold increase in cell viability in the printed constructs was observed on the seventh day of culture. The findings suggest that the developed bioink composition demonstrates significant potential for 3D-bioprinting-based biomedical applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144745"},"PeriodicalIF":7.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic multi-crosslinked networks in chitosan/Gallic acid/Fe3+ composite biofilms with high UV resistance and antioxidant properties 壳聚糖/没食子酸/Fe3+复合生物膜的协同多交联网络及其抗紫外线和抗氧化性能

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-28 DOI: 10.1016/j.ijbiomac.2025.144686

Hong Zhang, Xin Zhang, Bo Wang, Bo Ren, Xiaodong Yang

{"title":"Synergistic multi-crosslinked networks in chitosan/Gallic acid/Fe3+ composite biofilms with high UV resistance and antioxidant properties","authors":"Hong Zhang, Xin Zhang, Bo Wang, Bo Ren, Xiaodong Yang","doi":"10.1016/j.ijbiomac.2025.144686","DOIUrl":"10.1016/j.ijbiomac.2025.144686","url":null,"abstract":"<div><div>Food spoilage leads to plenty of food being wasted every year. Safe and multifunctional food preservation packaging is urgently required to prevent food from contamination. Chitosan/gallic acid/Fe<sup>3+</sup> (CSGA-Fe<sup>3+</sup>) composite films were synthesized by incorporating gallic acid (GA) and Fe<sup>3+</sup> into a chitosan solution. The integration of GA forms covalent and hydrogen bonds with chitosan, whereas Fe<sup>3+</sup> addition establishes metal-ligand interactions with both chitosan and GA, resulting in a film characterized by a multi-crosslinked network structure. This multi-crosslinked system substantially enhanced the material's mechanical properties and increased resistance to oxidation and ultraviolet degradation. The data indicate that the mechanical strength of chitosan/gallic acid (CSGA) films, with an inclusion of 0.3 mmol of GA, improved by 37.5 % over pure chitosan (CS) films. Moreover, the mechanical strength of the CSGA-Fe<sup>3+</sup> films saw a significant increase of 124.2 %, escalating from 1.32 MPa to 17.72 MPa. Additionally, the free radical scavenging capacities of the CSGA and CSGA-Fe<sup>3+</sup> films rose by 279.4 % and 255.0 %, respectively, compared to the pure CS film. The UV-blocking efficacy of the CSGA-Fe<sup>3+</sup> films, evaluated at 365 nm, reached 84.9 %. The water contact angle of the CSGA-1.5Fe<sup>3+</sup> film increased by 18.08 % compared to the CS film. This study provides innovative ideas to promote the broader application of safe and multifunctional chitosan-based composite films in food preservation packaging.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144686"},"PeriodicalIF":7.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational design of S-adenosylmethionine decarboxylase SpeD and spermidine synthase SpeE for green synthesis of spermidine 合理设计绿色合成亚精胺的s -腺苷蛋氨酸脱羧酶SpeE和亚精胺合成酶SpeE

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-28 DOI: 10.1016/j.ijbiomac.2025.144680

Ziyue Zhao , Dian Zou , AnYing Ji, Yingchao Wu, Ailing Guo, Xuetuan Wei

{"title":"Rational design of S-adenosylmethionine decarboxylase SpeD and spermidine synthase SpeE for green synthesis of spermidine","authors":"Ziyue Zhao , Dian Zou , AnYing Ji, Yingchao Wu, Ailing Guo, Xuetuan Wei","doi":"10.1016/j.ijbiomac.2025.144680","DOIUrl":"10.1016/j.ijbiomac.2025.144680","url":null,"abstract":"<div><div>Spermidine, a natural polyamine, possesses multiple biological activities and holds excellent application value. However, the low-activity enzymes in spermidine synthesis pathway limits spermidine production, including S-adenosylmethionine decarboxylase (SpeD) and spermidine synthase (SpeE). In <em>Bacillus amyloliquefaciens</em> PM1 with xylose as substrate, this study performed the alanine scanning mutagenesis to screen the beneficial mutants of SpeD and SpeE by rational design. Therein, the spermidine titers of mutants PM1/<em>speD</em><sup>I39A/D22A</sup> and PM1/<em>speE</em><sup>I108A/T54A</sup> were improved by 53 % and 44 % compared to the control strain, respectively, and the enzyme activities of the SpeD<sup>I39A/D22A</sup> and SpeE<sup>I108A/T54A</sup> increased by 58 % and 44 % accordingly. The mechanism of the enhanced enzymatic activity was further explained by molecular dynamics simulations. Moreover, the optimal engineering strain PM1::D/E was constructed by combination of <em>speD</em><sup>I39A/D22A</sup> and <em>speE</em><sup>I108A/T54A</sup> to enhance spermidine pathway. Through fed-batch fermentation, the maximum spermidine titer reached 683.14 mg/L, representing a 16.38-fold increase in spermidine production compared with the unmodified strain PM1. This study provides a novel strategy for green synthesis of spermidine from xylose, which will promote the clean and efficient production of spermidine.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144680"},"PeriodicalIF":7.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan/zinc oxide (ZnO) nanocomposites: A critical review of emerging multifunctional applications in food preservation and biomedical systems 壳聚糖/氧化锌纳米复合材料：在食品保鲜和生物医学系统中的新兴多功能应用综述

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-28 DOI: 10.1016/j.ijbiomac.2025.144773

Bekinew Kitaw Dejene , Mulat Alubel Abtew

{"title":"Chitosan/zinc oxide (ZnO) nanocomposites: A critical review of emerging multifunctional applications in food preservation and biomedical systems","authors":"Bekinew Kitaw Dejene , Mulat Alubel Abtew","doi":"10.1016/j.ijbiomac.2025.144773","DOIUrl":"10.1016/j.ijbiomac.2025.144773","url":null,"abstract":"<div><div>The increasing uses of petroleum-based materials for food packaging and medical applications raised significant environmental concerns. Researchers and industry stakeholders are actively exploring sustainable alternatives to replace those materials. Chitosan/zinc oxide (ZnO) bio-nanocomposites have emerged as promising alternatives to replace conventional plastics. Chitosan, a biodegradable biopolymer, exhibits exceptional film-forming properties, biocompatibility, and antimicrobial activity for sustainable application. On contrary, it has also certain drawbacks that limit its industrial applications including poor mechanical properties and high sensitivity to humidity. The integration of ZnO nanoparticles addresses these limitations by enhancing their mechanical strength, UV-blocking ability, stability, and functional properties with superior antimicrobial and antioxidant capabilities. This study critically examines past and current research, innovations, and development in chitosan/ZnO nanocomposites, with a focus on their sources, latest synthesis methods, properties, limitations and potentials applications in the food preservation and biomedical fields. Findings indicate that these nanocomposites can not only extend the shelf life of food products and are used for effective wound treatment, drug delivery, and tissue engineering but also reduces environmental impact. By highlighting recent advancements and future research directions, this review aims to promote the development of sustainable and eco-friendly solutions to address global challenges in food waste and healthcare.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144773"},"PeriodicalIF":7.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bamboo powder lignocellulose/polybutylene adipate terephthalate biodegradable bioplastic composite film for food packaging materials 竹粉木质纤维素/聚己二酸丁二酯可生物降解生物塑料复合薄膜，用于食品包装材料

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-28 DOI: 10.1016/j.ijbiomac.2025.144781

Zheng Cheng , Mei Ning , Kaiyun Mao , Xinmei Nong , Jialin Li , Naiyu Xiao , Xueqin Zhang , Qijun Ding , Honglei Wang , Meixian Liu

{"title":"Bamboo powder lignocellulose/polybutylene adipate terephthalate biodegradable bioplastic composite film for food packaging materials","authors":"Zheng Cheng , Mei Ning , Kaiyun Mao , Xinmei Nong , Jialin Li , Naiyu Xiao , Xueqin Zhang , Qijun Ding , Honglei Wang , Meixian Liu","doi":"10.1016/j.ijbiomac.2025.144781","DOIUrl":"10.1016/j.ijbiomac.2025.144781","url":null,"abstract":"<div><div>Herin, a biodegradable bioplastic composite packaging film was prepared by utilizing bamboo powder partially in replace of plastic. Bamboo powder lignocellulose and polybutylene adipate terephthalate (PBAT) resin granules were mixed together with certain percentage to form bamboo-plastic complex, and then through hot-pressed to obtain the bamboo/PBAT bioplastic composite films. The effect of bamboo powder content on overall properties of the composite film was systematically investigated. Results showed that the addition of bamboo powder could greatly improve the mechanical properties of composite films, especially the tensile strength and elastic modulus increased by 18.90 %, 251.58 %, respectively. Besides, the bioplastic composite film exhibited superior water resistance including the high water contact angle value of 108.13°, low water absorption rate (2.38 %), and water absorption thickness expansion rate (1.08 %) with 10.0 % bamboo powder content. Notably, the enhanced bonding between bamboo powder and PBAT contributed to the excellent gas barrier performance (1.48 × 10<sup>−2</sup> cm<sup>3</sup>·m/(m<sup>2</sup>·24 h·0.1 MPa)). With the increase of bamboo powder addition, the melt flow rate of the composite was increased, indicating the improved processing performance. More importantly, the bamboo/PBAT bioplastic composite film showed good packaging preservation ability for strawberry and excellent biodegradability in soil, presenting feasible and green alternatives to biodegradable plastic food packaging material.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144781"},"PeriodicalIF":7.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the formation mechanism of Tartary buckwheat starch-flavonoid complexes with different treatments 不同处理下苦荞淀粉类黄酮配合物形成机理的研究

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-28 DOI: 10.1016/j.ijbiomac.2025.144758

Yiming Zhou, Shunyu Li, Yi Huang, Xiaoli Zhou, Minglong Wang

{"title":"Study on the formation mechanism of Tartary buckwheat starch-flavonoid complexes with different treatments","authors":"Yiming Zhou, Shunyu Li, Yi Huang, Xiaoli Zhou, Minglong Wang","doi":"10.1016/j.ijbiomac.2025.144758","DOIUrl":"10.1016/j.ijbiomac.2025.144758","url":null,"abstract":"<div><div>The complexation of Tartary buckwheat starch with flavonoids facilitates the preparation of resistant starch. However, the research on the formation mechanism of Tartary buckwheat starch-flavonoid complexes under various treatment methods remains limited. In this study, we investigated the effects of high hydrostatic pressure treatment and heat treatment on Tartary buckwheat starch-flavonoid complexes (BSF), and explored the binding mechanisms between Tartary buckwheat starch (TBS) and flavonoids. The results demonstrated that the degree and type of BSF were significantly altered under different treatments. UV and fluorescence spectra analysis revealed that the binding mechanism of starch and flavonoids differed under high hydrostatic pressure (HHP) treatment compared to heat treatment. Interaction force studies indicated that in the HT complex, starch and flavonoids were primarily bonded via hydrogen bonds, whereas in the HHP treated complex, bonding occurred through both hydrogen bonds and hydrophobic interactions. The conformational changes, solubility, interaction energy, solvation free energy, and binding free energy of the complexes were systematically investigated using molecular dynamics simulations. High hydrostatic pressure facilitates complex formation through hydrogen bonding and hydrophobic interactions, with 400 MPa HHP exhibiting the highest binding energy (−49.93 kcal/mol). Heat treatment promotes complex formation predominantly through hydrogen bonding but with lower binding energy (−37.47 kcal/mol). Overall, HHP enables flavonoids to enter the starch spiral cavity, resulting in a more compact structure, while heat treatment induces starch despiralization of starch, thereby increasing the binding sites available for flavonoids.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144758"},"PeriodicalIF":7.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan coatings with modulated surface roughness for the inhibition of marine macrofouling 调节表面粗糙度的壳聚糖涂层对海洋大污染的抑制作用

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144713

Dongkun Yu , Xiaoyang Zhao , Albert Peralta Amores , Yuyi Wang , Joydeep Dutta , Jin-Long Yang , Fei Ye

{"title":"Chitosan coatings with modulated surface roughness for the inhibition of marine macrofouling","authors":"Dongkun Yu , Xiaoyang Zhao , Albert Peralta Amores , Yuyi Wang , Joydeep Dutta , Jin-Long Yang , Fei Ye","doi":"10.1016/j.ijbiomac.2025.144713","DOIUrl":"10.1016/j.ijbiomac.2025.144713","url":null,"abstract":"<div><div>Surface roughness is known to influence biofouling in marine environments. Different from the conventional antifouling coatings based on hydrophobic polymeric materials such as epoxy and polydimethylsiloxane (PDMS), in this study, naturally occurring compound benzophenone-3 (BP-3) modified chitosan (CS) biopolymer coatings with varied surface roughness were prepared and studied for their antifouling effect for the first time. By regulating the competitive reactions between the reactants, different condensation products can be obtained which result in varied root mean square (RMS) roughness of CS-BP-3 coatings from 7.6 nm to 270.0 nm. It is found that the reaction time influences more than the reaction temperature while defining the surface roughness of CS-BP-3 coatings. Specifically, the coatings of CS-BP-3 prepared from a 2-h reaction led to RMS roughness between 7.6 and 55.18 nm, whereas after 12-h reaction roughness between 82.6 and 270.0 nm were measured. Subsequent antifouling experiments indicate that rougher coatings have a more pronounced effect of preventing the settlement of plantigrade mollusk mimicking the lotus effect. This work demonstrates that bio-inspired materials can be designed to achieve desired functions for practical applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144713"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnesium-dependent phosphatase 1 (MDP1) interacts with WRKY 53 and protein phosphatase 2C 80 (PP2C80) to improve salt stress tolerance by scavenging reactive oxygen species in Salix psammophila 镁依赖性磷酸酶1 （MDP1）与WRKY 53和蛋白磷酸酶2C80 （PP2C80）相互作用，通过清除活性氧提高沙柳耐盐性

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144687

Jianbo Li , Yangfei Yang , Fei Wang , Qinghua Ma , Huixia Jia

{"title":"Magnesium-dependent phosphatase 1 (MDP1) interacts with WRKY 53 and protein phosphatase 2C 80 (PP2C80) to improve salt stress tolerance by scavenging reactive oxygen species in Salix psammophila","authors":"Jianbo Li , Yangfei Yang , Fei Wang , Qinghua Ma , Huixia Jia","doi":"10.1016/j.ijbiomac.2025.144687","DOIUrl":"10.1016/j.ijbiomac.2025.144687","url":null,"abstract":"<div><div>The roles of haloacid dehalogenase-like hydrolase (HAD) proteins in plants under salt stress remain largely unexplored. In the present study, we identified and functionally characterized <em>SpsMDP1,</em> a member of the HAD family, from <em>Salix psammophila</em>, which is a shrub adapted to desert environments<em>. SpsMDP1</em> was strongly upregulated by salt stress. Ectopic expression of <em>SpsMDP1</em> in <em>Arabidopsis</em> and poplar enhanced salt tolerance, with increased peroxidase activity and less ROS accumulation. Enhanced xylem development was in transgenic poplar plants overexpressing <em>SpsMDP1</em>. Moreover, Y2H, Co-IP, BiFC, and luciferase complementation analyses demonstrated that SpsPP2C80 can interact with SpsMDP1 both in vitro and in vivo<em>.</em> In addition, Y1H, EMSA, and transient expression analysis revealed that SpsWRKY53 is an upstream regulator of <em>SpsMDP1</em> and can directly bind to the W-box in the promoter region and activate its expression. Both <em>SpsWRKY53</em> and <em>SpsPP2C80</em> can increase salt stress tolerance by increasing the activity of antioxidant enzymes. Taken together, in our study we propose a model for the <em>SpsWRKY53</em>–<em>SpsMDP1</em>–<em>SpsPP2C80</em> module to defend against salt stress by scavenging reactive oxygen species. Our results provide a foundation for better understanding the function of <em>SpsMDP1</em> in response to salt in <em>S. psammophila</em> and identifying candidate genes for transgenic salt resistance breeding.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144687"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bionanoclusters of chitosan, indole acetic acid and cobalt chloride enhance seed germination in upland cotton by modulating glyoxylate cycle enzymes and biochemical responses 壳聚糖、吲哚乙酸和氯化钴的生物纳米团簇通过调节glyoxylate cycle酶和生化反应来促进陆地棉花种子萌发

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144735

Vikram Singh , Shiwani Mandhania , Rashi Datten , Ajay Pal , Vinod Saharan , Om Prakash Ghimire

{"title":"Bionanoclusters of chitosan, indole acetic acid and cobalt chloride enhance seed germination in upland cotton by modulating glyoxylate cycle enzymes and biochemical responses","authors":"Vikram Singh , Shiwani Mandhania , Rashi Datten , Ajay Pal , Vinod Saharan , Om Prakash Ghimire","doi":"10.1016/j.ijbiomac.2025.144735","DOIUrl":"10.1016/j.ijbiomac.2025.144735","url":null,"abstract":"<div><div>Seed priming is a quick and easy technique to boost seed germination, seedling development and plant growth. Among many exogenous agents, nanoparticles are one of the promising agents which enable the controlled delivery of multiple active agents simultaneously, improving efficiency and effectiveness. In the present investigation, bionanoclusters (BNCs) composed of cobalt (Co), indole-3-acetic acid (IAA), and chitosan were synthesized and employed as seed priming agents in cotton seeds to examine their effects on seed germination as well as associated phenotypic and biochemical changes. These BNCs demonstrated monodispersity, stability, and a slow release of Co and IAA. Seed priming with 100 mg/l BNCs significantly increased the germination by 16.33 % (<em>p</em> < 0.01). Compared to the control, seedlings from BNCs-primed seeds exhibited significantly longer roots (54.27 %) and greater biomass (28.96 %) by the 12th day of germination (<em>p</em> < 0.01). Additionally, BNCs priming increased the activities of key enzymes of the glyoxylate cycle, including isocitrate lyase (20.43 %) and malate synthase (29.17 %), as well as total soluble sugar content (42.27 %) on the 3rd day of germination to support seed germination. Results highlight the promising role of BNCs in improving seed germination to improve crop productivity.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144735"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling in-kernel starch multiscale structure and nutritional profiles of thermal remodeling whole grain black tartary buckwheat 热重塑全粒黑苦荞籽粒内淀粉多尺度结构及营养特性的研究

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI: 10.1016/j.ijbiomac.2025.144730

Mengna Zhang , Pinjia Chen , Fengwei Yang , Tian Zhao , Jie Wang , Shiqi Li , Zhigang Chen , Jianchang Jin , Jinyan Gong

{"title":"Unraveling in-kernel starch multiscale structure and nutritional profiles of thermal remodeling whole grain black tartary buckwheat","authors":"Mengna Zhang , Pinjia Chen , Fengwei Yang , Tian Zhao , Jie Wang , Shiqi Li , Zhigang Chen , Jianchang Jin , Jinyan Gong","doi":"10.1016/j.ijbiomac.2025.144730","DOIUrl":"10.1016/j.ijbiomac.2025.144730","url":null,"abstract":"<div><div>Thermal treatments can improve the processing suitability and nutritional utilization of cereals. In this study, the effects of three thermal treatments, namely heat fluidization, microwave, and baking, on the physical, nutritional, and structural properties of whole grain black Tartary buckwheat (BTB), as well as the multiscale structures and digestive characteristics of in-kernel starches, were evaluated. Thermal treatments induced grain expansion, surface cracking, and porous internal structures, with heat fluidization causing the most significant reduction (32.06 %) in kernel hardness. Heat fluidization also enhanced rutin extractability by 68.00 % and antioxidant activities. Volatile compound analysis revealed heat fluidization reduced aldehyde and hydrocarbon levels, especially reducing hexanal from 27.06 to 3.86 mg/kg. For in-kernel starches, heat fluidization increased cold-water viscosity (56-fold), disrupted A-type crystallinity (relative crystallinity dropped to 8.49 %), reduced short-range ordered structure (R<sub>993/1018</sub> decreased by 2.21 %), and enhanced resistant starch content (33 %). The study highlighted heat fluidization as the most effective method for enhancing processing and nutritional properties of whole grain BTB, offering insights for developing nutrient-dense whole grain products.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"316 ","pages":"Article 144730"},"PeriodicalIF":7.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0