International Journal of Biological Macromolecules最新文献_第2页

Starch nanoparticle loading cinnamon essential oil encapsulated by complex coacervation of chitosan and gum Arabic improve the stability and antibacterial potential of the microcapsules 壳聚糖与阿拉伯胶复合凝聚包封肉桂精油后，淀粉纳米粒负载肉桂精油，提高了微胶囊的稳定性和抗菌潜力

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143613

Yang Yang , Wenjie Yang , Xingyu Chen , Huaxi Xiao , Gao-Qiang Liu , Wenfang Han , Jiangtao Li

{"title":"Starch nanoparticle loading cinnamon essential oil encapsulated by complex coacervation of chitosan and gum Arabic improve the stability and antibacterial potential of the microcapsules","authors":"Yang Yang , Wenjie Yang , Xingyu Chen , Huaxi Xiao , Gao-Qiang Liu , Wenfang Han , Jiangtao Li","doi":"10.1016/j.ijbiomac.2025.143613","DOIUrl":"10.1016/j.ijbiomac.2025.143613","url":null,"abstract":"<div><div>Essential oils have excellent antibacterial effects, but the rapid volatility of essential oils reduces their antibacterial effects. Therefore, the adsorption and slow release behavior of essential oils are of great significance for food preservation. This study used starch nanoparticles (SN) with different average particle sizes as carriers to adsorb cinnamon essential oil (CEO) and obtain starch nanoparticle - cinnamon essential oil (CEO-SN) complexes. Chitosan (CS) and Arabic gum (GA) composite films were used as wall materials, and sodium tripolyphosphate (STPP) was used as a curing crosslinking agent to encapsulate CEO-SN, forming CEO microcapsules (CM-CEO-SN). The optimal conditions of preparing CM-CEO-SN were determined by a single-factor experiment and response surface methodology. The thermal stability, morphology, in vitro release and antibacterial potential of the microcapsules were examined using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric differential thermal analysis and differential scanning calorimetry. The results showed that the release rate of CM-CEO-SN300 decreased by 33.7 % and had the better slow release effect and thermal stability. In addition, CM-CEO-SN300 effectively inhibited the spoilage of cherry tomatoes during storage. These findings provide useful guidance for extending food shelf life.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143613"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

m6A modification profiles of the CHO cells with differential recombinant protein expression using MeRIP-seq/RNA-seq 利用MeRIP-seq/RNA-seq技术对具有差异重组蛋白表达的CHO细胞进行m6A修饰谱分析

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143429

Wen Wang , Hai-Tong Wang , Yang Guo , Qi Zhao , Jiang-Tao Lu , Zhao-Ming Cui , Xi Zhang , Le-Le Qiu , Xiao-Yin Wang , Tian-Yun Wang , Yan-Long Jia

{"title":"m6A modification profiles of the CHO cells with differential recombinant protein expression using MeRIP-seq/RNA-seq","authors":"Wen Wang , Hai-Tong Wang , Yang Guo , Qi Zhao , Jiang-Tao Lu , Zhao-Ming Cui , Xi Zhang , Le-Le Qiu , Xiao-Yin Wang , Tian-Yun Wang , Yan-Long Jia","doi":"10.1016/j.ijbiomac.2025.143429","DOIUrl":"10.1016/j.ijbiomac.2025.143429","url":null,"abstract":"<div><div>Chinese hamster ovary (CHO) cells remain the primary host system for recombinant therapeutic protein production. Enhancing transgene expression efficiency while maintaining stable production persists as a key challenge in CHO cell engineering. While N6-methyladenosine (m6A) modification – the most abundant RNA methylation – regulates RNA stability and translational efficiency, its role in modulating recombinant protein expression remains underexplored. In this study, through m6A-specific methylated RNA immunoprecipitation sequencing (MeRIP-seq) of high- (ADM-H) and low- (ADM-L) recombinant adalimumab (ADM)-producing CHO cell lines, we identified 668 differentially methylated peaks. Notably, m6A methylation patterns showed positive correlation with heavy chain (HC)/light chain (LC) expression levels between ADM-H and ADM-L cell lines. Differential expression of factors, such as Igf2bp2, Gli2, and Met correlated with PI3K-Akt and Hippo signaling pathways, suggesting m6A-mediated regulatory functions of recombinant protein expression in CHO cells. Furthermore, pharmacological inhibition of Gli2 or Met in cell culture effectively enhanced ADM production while suppressing target gene expression. These findings elucidate m6A's functional role in recombinant protein production and provide actionable strategies for CHO cell line optimization.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143429"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of highly tough, self-healing sodium alginate/polyacrylamide and copper based nanocomposite hydrogel and its application as strain and pressure sensor for human health monitoring and signature recognition 高韧性自愈海藻酸钠/聚丙烯酰胺和铜基纳米复合水凝胶的制备及其在人体健康监测和特征识别中的应变和压力传感器应用

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143734

Usman Muhammad , Xuan Cao , Taoyi Zhang , Wenxi Ji , Rulong Lv , Jing Chen , Yun Wei

{"title":"Fabrication of highly tough, self-healing sodium alginate/polyacrylamide and copper based nanocomposite hydrogel and its application as strain and pressure sensor for human health monitoring and signature recognition","authors":"Usman Muhammad , Xuan Cao , Taoyi Zhang , Wenxi Ji , Rulong Lv , Jing Chen , Yun Wei","doi":"10.1016/j.ijbiomac.2025.143734","DOIUrl":"10.1016/j.ijbiomac.2025.143734","url":null,"abstract":"<div><div>Conductive hydrogel-based strain and pressure sensors have been extensively employed in various fields such as soft robotics and human-machine interaction. Nonetheless, it still remains challenging to synthesize a conductive hydrogel with exquisite mechanical properties, electrical conductivity and sensitivity. Herein, a novel double network nanocomposite conductive hydrogel was fabricated by using sodium alginate (SA), polyacrylamide (PAm) and copper metal nanoparticles (CuNPs) and further utilized to construct highly sensitive strain and pressure sensors. The optimized SA:PAm/CuNPs-18 hydrogel exhibited a tensile strength of 0.42 MPa, an elongation at break of 1448 %, a toughness of 3.90 MJ m<sup>−3</sup> and an electrical conductivity of 2.4 S m<sup>−1</sup>. Furthermore, the SA:PAm/CuNPs-18 hydrogel-based strain sensor was successfully utilized for multi-scale sensing and monitoring of the movements of elbow joint, knee joint, wrist joints, neck muscles, facial expressions and pulse of humans. In addition, the SA:PAm/CuNPs-18 hydrogel-based pressure sensor also showed great potential to detect and differentiate handwritten letters of English even at variable applied pressures and speeds. All these results indicate that the strain and pressure sensors can be integrated in wearable electronic devices, which are useful in medical observation and accurate signature recognition of humans.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143734"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Starch granular architecture of three typical Chinese indica rice cultivars (Huiliangyou 16, Liangyou 6176 and Zhongzao 83) and its impact on textural properties of rice noodles 3个典型籼稻品种徽优16号、两优6176和中早83淀粉颗粒结构及其对米粉质构特性的影响

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143741

Kai Du , Xuefei Yang , Hanwen Hu , Xiyang Zhong , Shuizhong Luo , Liang Wu , Caishuo Bao , Zhi Zheng

{"title":"Starch granular architecture of three typical Chinese indica rice cultivars (Huiliangyou 16, Liangyou 6176 and Zhongzao 83) and its impact on textural properties of rice noodles","authors":"Kai Du , Xuefei Yang , Hanwen Hu , Xiyang Zhong , Shuizhong Luo , Liang Wu , Caishuo Bao , Zhi Zheng","doi":"10.1016/j.ijbiomac.2025.143741","DOIUrl":"10.1016/j.ijbiomac.2025.143741","url":null,"abstract":"<div><div>In this work, three typical Chinese indica rice cultivars, Huiliangyou 16 (Hly 16), Liangyou 6176 (Ly 6176), and Zhongzao 83 (Zz 83), were analyzed to assess the synergistic effects of their starch granular architecture on the production and textural properties of rice noodles. The thermal, pasting and rheological properties of rice starches were significantly influenced by their amylose content and amylopectin chain length distribution. Comparatively, the starch isolated from Zz 83 exhibited the highest amylose content (29.76 %), B3-chains content (13.38 %) and molecular weight (140,996.49 kDa), as well as the lowest relative crystallinity (15.11 %) and A-chains content (22.33 %). Rice noodles made from Zz 83 demonstrated excellent textural properties (hardness, 3816.31 g; springiness, 0.94; cohesiveness, 0.74; gumminess, 2741.36; chewiness, 2529.49; resilience, 0.43), which was attributed to the starch extracted from Zz 83 exhibiting the rheological properties of large storage modulus (G′) and small tan δ values. Taken together, the formation of high-quality rice noodles was ascribed to the combined effects of the physicochemical and structural properties of rice starches. These findings may provide valuable insights into the contribution of starch to the preparation of premium-quality rice noodles.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143741"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A complex interplay between alginate/pectin and rice varieties in determining the starch digestibility of reconstituted rice beads 海藻酸盐/果胶与水稻品种之间的复杂相互作用决定了重组水稻珠的淀粉消化率

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143809

Senbin Cao, Cheng Li

{"title":"A complex interplay between alginate/pectin and rice varieties in determining the starch digestibility of reconstituted rice beads","authors":"Senbin Cao, Cheng Li","doi":"10.1016/j.ijbiomac.2025.143809","DOIUrl":"10.1016/j.ijbiomac.2025.143809","url":null,"abstract":"<div><div>Non-starch polysaccharide (NSP) encapsulated rice flour is a novel type of rice analogue, while the influence of different NSPs and rice varieties on starch digestibility remains unknown. Therefore, three rice varieties with different structures were encapsulated by alginate or pectin, and their digestibility and textures were investigated with regards to the alterations of their multi-scale structures during digestion. An approximately 1.9 times difference of enzymatic binding rate constant and 10 % difference of the maximum starch digested percentage were found among different rice beads. Nanjing rice beads showed slower starch digestibility when encapsulated by alginate, while Panjin and Subei rice showed the opposite. Alginate encapsulated rice beads showed a harder texture than pectin encapsulated ones, although the texture also depended on rice varieties. Mechanistically, amylopectin chains with a peak DP around 15 from Nanjing rice were proposed to interact with alginate, thus reducing its digestibility. In comparison, the large pores formed in pectin encapsulated Panjin and Subei rice beads after cooking promoted the re-association of starch molecules into more ordered structure and thus reduced digestibility. These results suggest that careful selection of both NSP types and rice varieties is required to develop rice beads/analogues with desirable starch digestibility.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143809"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stabilization of high internal phase emulsions by combination of polysaccharide and protein: Synergistic efficiency, mechanism, and application 多糖与蛋白质复合稳定高内相乳剂：协同效率、机理及应用

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143544

Yuqian Zhu , Yubo Zhu , Jingchen Yang, Yanbo Wang, Chen Tan

引用次数: 0

Novel bioink derived from low methoxyl pectin, gelatin and aloe vera as natural biomaterials for fabricating scaffolds encapsulated with living cells by 3D bioprinting 新型生物胶来源于低甲氧基果胶，明胶和芦荟，作为天然生物材料，用于通过生物3D打印制造包裹活细胞的支架

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143364

Jirasak Jitpibull , Thunyaporn Ravangnam , Nalinrat Petpiroon , Wichaya Kalaithong , Kanittha Boonpavanitchakul , Sasitorn Aueviriyavit , Wiyong Kangwansupamonkon

{"title":"Novel bioink derived from low methoxyl pectin, gelatin and aloe vera as natural biomaterials for fabricating scaffolds encapsulated with living cells by 3D bioprinting","authors":"Jirasak Jitpibull , Thunyaporn Ravangnam , Nalinrat Petpiroon , Wichaya Kalaithong , Kanittha Boonpavanitchakul , Sasitorn Aueviriyavit , Wiyong Kangwansupamonkon","doi":"10.1016/j.ijbiomac.2025.143364","DOIUrl":"10.1016/j.ijbiomac.2025.143364","url":null,"abstract":"<div><div>In recent years, 3D bioprinting has emerged as a promising technology for developing complex tissue structures, precisely controlled and designed <em>via</em> computer systems to create intricate organ model. This study focuses on the development of a bioink composed of living cells and hydrogel, specifically using low methoxyl pectin (LMP) combined with gelatin to enhance the properties of bioink for tissue engineering applications. Additionally, aloe vera (AV) dry gel was incorporated into the bioink to promote cell proliferation. The rheological properties of the developed hydrogels were evaluated, revealing shear-thinning and thixotropic behaviors suitable for 3D bioprinting applications. FT-IR and TGA analyses were performed to investigate the chemical and thermal properties of the hydrogels, indicating interactions between the constituents. The printability assessment demonstrated that the developed hydrogels (LMP/Gel, LMP/Gel-1AV, LMP/Gel-2AV) were able to maintain accurate shapes with over 90 % precision. Morphological observation of the dried hydrogel revealed a porous structure with interconnected pores, ensuring nutrient diffusion throughout the hydrogel. Furthermore, the developed bioinks demonstrated biodegradability, low toxicity, and the successful formation of an epidermal-like structure, further highlighting their potential for tissue engineering applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143364"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional loofah-inspired microfibrillated cellulose aerogel with excellent thermal insulation and sorption properties 多功能丝瓜络启发微纤化纤维素气凝胶具有优异的隔热和吸附性能

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143737

Wei Qin , Huimin Zhang , Ju Wei , Yitong Chen , Yongping Liao , Xiaoxiao Wei , Meifeng Guo , Jialing Huang , Hua Shen

{"title":"Multifunctional loofah-inspired microfibrillated cellulose aerogel with excellent thermal insulation and sorption properties","authors":"Wei Qin , Huimin Zhang , Ju Wei , Yitong Chen , Yongping Liao , Xiaoxiao Wei , Meifeng Guo , Jialing Huang , Hua Shen","doi":"10.1016/j.ijbiomac.2025.143737","DOIUrl":"10.1016/j.ijbiomac.2025.143737","url":null,"abstract":"<div><div>Biomass aerogels have garnered increasing attention recently due to their wide-ranging applications. Yet, developing cost-effective and versatile aerogel materials remains challenging, as conventional biomass material production processes are complex and environmentally unfriendly. Loofah fiber, a porous cellulose fiber with a natural three-dimensional structure, has been recognized as a new sustainable resource in recent years. In this study, we adopted an innovative approach to fabricate a structurally stable and controllable loofah-derived cellulose aerogel by directly using natural agricultural fibers through simple mechanical treatment and freeze-drying. The achieved multifunctional aerogels exhibited high porosity (99.2 %), hydrophobicity (140.1°), self-cleaning, good mechanical performance, outstanding sorption capacity (40–190 g/g of oil and organic solvents) and thermal insulation (0.030 W/(m·K)) properties. Meanwhile, the aerogel also possessed excellent economy, reusability, and sustainability. This study paves the way for designing next-generation natural biomass-based sorbing and thermal insulation porous materials with great potential for multifunctional applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"311 ","pages":"Article 143737"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physicochemical aspects of hydrogel preparation from algal cellulose 从藻类纤维素制备水凝胶的物理化学方面

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143499

Konstantin Bogolitsyn , Anastasia Parshina , Nikolai Novoselov , Anton Muravyev , Elena Abramova , Sergey Khviuzov , Semyon Shestakov , Alexander Kozhevnikov

{"title":"Physicochemical aspects of hydrogel preparation from algal cellulose","authors":"Konstantin Bogolitsyn , Anastasia Parshina , Nikolai Novoselov , Anton Muravyev , Elena Abramova , Sergey Khviuzov , Semyon Shestakov , Alexander Kozhevnikov","doi":"10.1016/j.ijbiomac.2025.143499","DOIUrl":"10.1016/j.ijbiomac.2025.143499","url":null,"abstract":"<div><div>Brown algae represent a valuable and promising source of cellulosic materials due to their high productivity and widespread, low cultivation cost and ease of processing, attributable to the absence of lignin. The aforementioned advantages are accompanied by the unique properties of algal cellulose (low degree of polymerization and an Iα-type crystal cell structure) due to the peculiarities of biosynthesis. These properties render algae superior to higher plants in terms of producing pure, mesoporous cellulosic materials, e.g. gels. The aim of this study is to first time evaluate the hydration capacity of algal cellulose and nanocellulose during hydrogel formation. Employing FTIR spectroscopy, calorimetry, and nuclear magnetic resonance relaxometry, we explored the interaction of algal cellulose and nanocellulose with water. Nanocellulose has a higher content of free water owing to its developed mesoporous structure. The interaction between cellulose and water is exothermic, liberating heat at 7.2–29.2 J/g d.w. The high moisture retention capacity (37 g/g), coupled with the small size of nanocrystals, facilitates the formation of a stable homogeneous algal nanocellulose hydrogel, which remains stable over extended storage periods. This resultant hydrogel has promising applications in biomedical material production, including wound dressings, anti-adhesive films, and abdominal surgery gels.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143499"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BioSeq_Ksite: Multi-perspective feature-driven prediction of protein succinylation based on an adaptive attention module with SSBCE loss strategy BioSeq_Ksite：基于SSBCE丢失策略的自适应注意力模块的蛋白质琥珀酰化多视角特征驱动预测

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI: 10.1016/j.ijbiomac.2025.143601

Lun Zhu , Ziqi Zhang , Sen Yang

{"title":"BioSeq_Ksite: Multi-perspective feature-driven prediction of protein succinylation based on an adaptive attention module with SSBCE loss strategy","authors":"Lun Zhu , Ziqi Zhang , Sen Yang","doi":"10.1016/j.ijbiomac.2025.143601","DOIUrl":"10.1016/j.ijbiomac.2025.143601","url":null,"abstract":"<div><div>Succinylation is a post-translational modification in which a succinyl group is transferred to the lysine residue of a protein, playing a crucial role in regulating both protein structure and cellular function. This paper introduces a novel sequential model, BioSeq_Ksite, designed to enhance succinylation prediction accuracy by integrating an adaptive attention mechanism and a joint loss function. This study first presents a new hybrid feature, ProtFusion, which combines the physicochemical properties of amino acids with pretrained models. Next, this paper introduces an adaptive attention module that enables the model to autonomously identify important features during training. Additionally, a gated network architecture is adopted to create a dual-branch sequential model. Finally, by combining sensitivity, specificity, and cross-entropy loss, a new joint loss function is proposed, which is used for succinylation prediction for the first time and significantly enhances the model's ability to handle class-imbalanced data. Evaluation on the test dataset shows that BioSeq_Ksite outperforms other models in MCC, Sn, AUC, and F1-Score, with a 7.68 % improvement in MCC over the second-best model. It provides an efficient and reliable tool for succinylation research and application. BioSeq_Ksite can be accessed at <span><span>https://github.com/zzq1124ZHZ/BioSeq_Ksite</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143601"},"PeriodicalIF":7.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0