International Journal of Biological Macromolecules最新文献_第2页

Effect of bioactive Rosa roxburghii Tratt fruit polysaccharide on the structure and emulsifying property of lactoferrin protein.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140016

Wang Pingping, Du Yutong, Chai Xianghua, Chen Chun, Wu Kegang, Fu Xiong

{"title":"Effect of bioactive Rosa roxburghii Tratt fruit polysaccharide on the structure and emulsifying property of lactoferrin protein.","authors":"Wang Pingping, Du Yutong, Chai Xianghua, Chen Chun, Wu Kegang, Fu Xiong","doi":"10.1016/j.ijbiomac.2025.140016","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140016","url":null,"abstract":"Lactoferrin protein (LF) is a natural protein with certain emulsifying ability, but is sensitive to be affected by environmental factors and has poor oxidative stability to be used as emulsifier. In this study, the emulsifying ability of LF was significantly improved after conjugation with Rosa roxburghii Tratt fruit polysaccharides (RTFP), and the emulsion stability mechanism of LF-RTFP conjugates (L-R) were elucidated through the utilization of CLSM (confocal laser scanning microscopy), interfacial tension, apparent viscosity, and protein adsorption rate. The emulsion stabilized by L-R showed the smaller particle size (17.17 ± 0.13 μm), which reduced by 51 % compared with that of LF. After conjugation with RTFP, the hydrophobic amino acids that are originally inside the structure of LF would be exposed on the protein surface. In addition, the protein adsorption rate of L-R stabilized emulsion interface was 62.70 ± 0.71 %, 2.4 times higher than that of LF. The optical microscopy and CLSM experiments indicated that the glycosylation with RTFP increased the bridged flocculation and further formed gel network structure in the emulsion stabilized by LF. These findings suggested that the novel emulsifier prepared by the Maillard reaction between LF and RTFP showed potential to stable emulsion.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140016"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication and characterization of emulsion stabilized by tannic acid/soluble potato starch complexes.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.139904

Xianling Wei, Rui Lei, Ziqing Hu, Weidong Bai, Xiaofang Zeng, Xiaoyan Liu, Huan Xie, Jieyu Chen

{"title":"Fabrication and characterization of emulsion stabilized by tannic acid/soluble potato starch complexes.","authors":"Xianling Wei, Rui Lei, Ziqing Hu, Weidong Bai, Xiaofang Zeng, Xiaoyan Liu, Huan Xie, Jieyu Chen","doi":"10.1016/j.ijbiomac.2025.139904","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139904","url":null,"abstract":"In this study, the influence of tannic acid (TA)/soluble potato starch (PS) mass ratio and PS concentration on TA/PS complexes and emulsions stabilized by TA/PS complexes were studied. The size, hydrophobicity and emulsifying properties of TA/PS complexes were all controlled by TA/PS mass ratio and PS concentration. In detail, the hydrophobicity of PS (θow = 48°) improved after complexing with TA to form TA/PS complexes (θow max = 64°). The emulsions size decreased and then increased with increasing TA/PS ratio. Additionally, the emulsifying properties of TA/PS complexes improved by increasing PS concentration. Analysis of the interfacial tension after adsorption equilibrium (0.25 mass ratio, TA/PS complexes (13.03 mN/m), TA (14.21 mN/m) and PS (20.25mN/m)), TA and PS had a synergistic effect of stabilizing the oil-water surface. Among them, TA mainly played a role in emulsifying property, and PS mainly played a role of stabilization. All emulsions exhibited obvious creaming. However, at high PS concentration or TA/PS ratio, the creaming was prevented by formed smaller emulsion size, interface complexes networks or high viscosity (Increased from 0.004 to 0.060 Pa.s). It showed that TA/PS complexes can act as emulsifiers to improve the physical and oxidative stability of emulsions, making them suitable for delivering oxidation-sensitive fat-soluble bioactive compounds.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139904"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of chitosan-capped quercetin nanoparticles on chitosan/poly(vinyl) alcohol multifunctional films: A sustainable approach for bread preservation.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140029

Manjushree Nagaraj Gunaki, Saraswati P Masti, Lingaraj Kariyappa Kurabetta, Jennifer P Pinto, Ajitkumar Appayya Hunashyal, Nagarjun Prakash Dalbhanjan, Ravindra B Chougale, S K Praveen Kumar

{"title":"Influence of chitosan-capped quercetin nanoparticles on chitosan/poly(vinyl) alcohol multifunctional films: A sustainable approach for bread preservation.","authors":"Manjushree Nagaraj Gunaki, Saraswati P Masti, Lingaraj Kariyappa Kurabetta, Jennifer P Pinto, Ajitkumar Appayya Hunashyal, Nagarjun Prakash Dalbhanjan, Ravindra B Chougale, S K Praveen Kumar","doi":"10.1016/j.ijbiomac.2025.140029","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140029","url":null,"abstract":"Food packaging industries are growing to meet consumer demand and prevent pollution by adopting significant biopolymer advancements. Therefore, this study aimed to develop functionally active chitosan (CS)/polyvinyl alcohol (PVA)-based biopolymer films and evaluate the effect of Justicia Adhatoda extract (JAE), pure quercetin (Q), and CS-capped quercetin nanoparticles ((Q)CS NPs) on sustainable bread packaging. CS was successfully loaded onto (Q) by the one-pot method, which was confirmed by light absorption spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The fabricated films were examined using different analytical techniques. FTIR and XRD patterns confirmed that the CS/PVA matrix had molecular interactions through hydrogen bonds with JAE, (Q), and (Q)CS NPs. SEM micrograms revealed a uniform distribution and denser surface with small aggregations by adding (Q)CS NPs. The (Q)CS NPs added CPE(Q)CS nanocomposite exhibited excellent UV light shielding (99.99 % UV-A and UV-B blocking), water resistance ability (contact angle:99.44°, WVP:3.68×10-7 gh-1m-1Pa-1), and oxygen (0.614×10-6gh-1 m-1 atm-1) barrier properties. Adding (Q)CS NPs enhanced the antimicrobial properties of CPE(Q)CS against foodborne microbes (E. coli:15.45 mm, P. aeruginosa:14.50 mm, B. subtilis:14.25 mm, S. aureus:13.52 mm, and C. albicans:15.16 mm). In addition, incorporating (Q)CS NPs, increased the shelf life of bread compared to unpacked and polyethylene-packed bread samples.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140029"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyaluronan-coated gold nanoshells for enhanced synergistic effect and immunogenic cell response of chemo-photothermal therapy on lung cancer.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140114

Ke-Cheng Chen, Shu-Jyuan Yang, Shih-Hung Yang, Jui-An Pai, Ming-Jium Shieh

{"title":"Hyaluronan-coated gold nanoshells for enhanced synergistic effect and immunogenic cell response of chemo-photothermal therapy on lung cancer.","authors":"Ke-Cheng Chen, Shu-Jyuan Yang, Shih-Hung Yang, Jui-An Pai, Ming-Jium Shieh","doi":"10.1016/j.ijbiomac.2025.140114","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140114","url":null,"abstract":"Lung cancer (LC) is the predominant cause of cancer-related fatalities globally, with the highest death rates in both genders, primarily attributed to smoking. The non-kinase transmembrane cell surface glycoprotein, CD44, enhances LC cell migration and invasion, leading to drug resistance and an unfavorable prognosis. This research formulated a cisplatin-loaded gold nanoshell (HCP@GNS) integrated with hyaluronan (HCP@GNS@HA) to enhance targeting capability and realize a synergistic effect of chemo-photothermal therapy (chemo-PTT) against LC. The coating of hyaluronic acid (HA) facilitated the uptake of HCP@GNS@HA into CD44-rich cancer cells, maintaining the superior photothermal conversion capacity of HCP@GNS nanoparticles for hyperthermia and photothermal eradication of tumor tissues under near-infrared exposure. As a nanocarrier, HCP@GNS@HA exhibited high biocompatibility and hemocompatibility, showing stronger cytotoxicity than either the free drug or photothermal therapy alone when combined with NIR irradiation, especially at high cis-diamminedichloroplatinum (II) (CDDP) concentrations. The chemo-PTT mediated by HCP@GNS@HA effectively curtailed tumor growth without adverse effects, significantly mobilizing B cells, DC cells, macrophages, natural killer (NK) cells, and NKT cells in the distal tumor against tumor growth. In conclusion, the developed hyaluronic acid (HA)-coated gold nanoshells could potentially serve as a promising candidate for nanomedicine, tackling both primary and distal LC growth.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140114"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Preparation of cellulose-based fluorescent aggregations with various morphologies and their microstructure-correlated fluorescence behavior" [Int. J. Biol. Macromol., Volume 290 (2025) 139015].

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.139955

Yijun Yao, Dong Fu, Kexin Hao, Yuan Xiao, Miao Wang, Guangbing Wei, Hailiang Wu

引用次数: 0

Conformational flexibility of human ribokinase captured in seven crystal structures.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140109

Naomi N Akanmori, Murray S Junop, Radhey S Gupta, Jaeok Park

{"title":"Conformational flexibility of human ribokinase captured in seven crystal structures.","authors":"Naomi N Akanmori, Murray S Junop, Radhey S Gupta, Jaeok Park","doi":"10.1016/j.ijbiomac.2025.140109","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140109","url":null,"abstract":"d-ribose is a critical sugar substrate involved in the biosynthesis of nucleotides, amino acids, and cofactors, with its phosphorylation to ribose-5-phosphate by ribokinase (RK) constituting the initial step in its metabolism. RK is conserved across all domains of life, and its activity is significantly enhanced by monovalent metal (M+) ions, particularly K+, although the precise mechanism of this activation remains unclear. In this study, we present several crystal structures of human RK in both unliganded and substrate-bound states, offering detailed insights into its substrate binding process, reaction mechanism, and conformational changes throughout the catalytic cycle. Notably, bound ATP exhibited significant conformational flexibility in its triphosphate moiety, a feature shared with other RK homologues, suggesting that achieving a catalytically productive triphosphate configuration plays a key role in regulating enzyme activity. We also identified a unique conformational change in the M+ ion binding loop of human RK, specifically the flipping of the Gly306-Thr307 peptide plane, likely influenced by the ionic radius of the bound ion. These findings provide new insights into the RK reaction mechanism and its activation by M+ ions, paving the way for future investigations into the allosteric regulation of human RK and related sugar kinase enzymes.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140109"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of curcumin-conjugated pH-responsive lignin-based nanoparticles for alleviating oxidative stress: Stability, antioxidant activity and biocompatibility.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140036

Huanli Gao, Farzad Seidi, Yuqun Cai, Ziwei Sun, Huiyang Bian, Hongqi Dai, Tingting Xu

引用次数: 0

Bioactive nanocellulose films by incorporation of enzymatically polymerized lignin nanoparticles.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140051

Luisa L García-Fuentevilla, Raquel Martín-Sampedro, Margarita Darder, David Ibarra, María E Eugenio

{"title":"Bioactive nanocellulose films by incorporation of enzymatically polymerized lignin nanoparticles.","authors":"Luisa L García-Fuentevilla, Raquel Martín-Sampedro, Margarita Darder, David Ibarra, María E Eugenio","doi":"10.1016/j.ijbiomac.2025.140051","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140051","url":null,"abstract":"In the search of new bioactive and biobased films, the use of lignin nanoparticles (LNP) and cellulose nanofibers (CNF) has gained potential relevance in the last years. In this context, an enzymatic and environmentally friendly pretreatment with laccases has been proposed in this work to modify the properties of the developed cellulose-lignin nanocomposite films. Thus, the laccase treatment successfully polymerized kraft lignin as indicated by the increase in weight average molecular weight (from 3621 to 5681 Da) and the reduction in phenol content (from 552 to 324 mg GAE/g lignin). Moreover, this polymerization also caused a significant reduction in the size of the resulting LNP (6.8 ± 2.4 nm) compared to those obtained from untreated lignin (62 ± 22 nm). The incorporation of both untreated and treated LNP conferred antioxidant, antibacterial and UV-shielding capabilities to the final LNP-CNF films, observing higher antioxidant and UV-shielding values with polymerized LNP probably due to its tiny size and conjugated functional groups, respectively. Furthermore, films with 5 % LNP also showed better thermal stability, elongation at break, water vapor permeability and transparency, compared to CNF control films.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140051"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erucic acid promotes intramuscular fat deposition through the PPARγ-FABP4/CD36 pathway.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140121

Hengwei Yu, Juntao Guo, Bingzhi Li, Jing Ma, Belete Kuraz Abebe, Chugang Mei, Sayed Haidar Abbas Raza, Gong Cheng, Linsen Zan

{"title":"Erucic acid promotes intramuscular fat deposition through the PPARγ-FABP4/CD36 pathway.","authors":"Hengwei Yu, Juntao Guo, Bingzhi Li, Jing Ma, Belete Kuraz Abebe, Chugang Mei, Sayed Haidar Abbas Raza, Gong Cheng, Linsen Zan","doi":"10.1016/j.ijbiomac.2025.140121","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140121","url":null,"abstract":"The regulation of intramuscular fat (IMF) accumulation plays a crucial role in determining meat quality in the beef industry. In humans, fat deposition in skeletal muscle is closely associated with insulin resistance and obesity. However, its underlying mechanisms are not fully elucidated. We previously identified erucic acid (EA) as a key metabolite that may affect IMF deposition of beef using omics strategies. By utilizing bovine intramuscular preadipocytes in vitro, the study demonstrates a dose-dependent increase in lipid storage induced by EA, along with mRNA expression levels of transporters FABP4 and CD36. At a mechanistic level, EA triggers ERK1/2 phosphorylation and enhances the expression of PPARγ, FABP4, and CD36, thereby facilitating the formation of lipid droplets within preadipocytes. In vivo experiments conducted in mice support these findings, indicating that EA stimulates fat accumulation in skeletal muscles and enhances the levels of FABP4 and CD36 proteins. These outcomes not only enhance our comprehension of the molecular mechanisms governing IMF deposition but also provide insights into potential strategies for enhancing meat quality and addressing metabolic disorders linked to fat accumulation in skeletal muscles. The findings of the study contribute to existing scholarly knowledge and lay the groundwork for future research endeavors aimed at improving meat quality and metabolic well-being.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140121"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of soy/pea protein isolate-oil interactions on the physicochemical and structural properties of high-moisture texturized proteins under different oil concentrations.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140026

Ji-Yeun Lee, Hyun Woo Choi, Myeongsu Jo, Hyung Joo Kim, Jungwoo Hahn, Young Jin Choi

{"title":"Impact of soy/pea protein isolate-oil interactions on the physicochemical and structural properties of high-moisture texturized proteins under different oil concentrations.","authors":"Ji-Yeun Lee, Hyun Woo Choi, Myeongsu Jo, Hyung Joo Kim, Jungwoo Hahn, Young Jin Choi","doi":"10.1016/j.ijbiomac.2025.140026","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140026","url":null,"abstract":"This study investigated the effects of oil addition on the physical and chemical properties of high-moisture texturized proteins (HMTPs), focusing on soy protein isolate (SPI) and pea protein isolate (PPI). Rheological analysis revealed contrasting behaviors: SPI exhibited decreased rheological parameters at low oil concentrations (1, 3 %), followed by a significant increase at higher concentrations (5, 10 %), whereas PPI showed a consistent decline across all oil concentrations. The superior emulsifying and gelling abilities of SPI resulted in stronger protein-protein interactions and greater hardness at higher oil concentrations. In contrast, PPI exhibited minimal protein-oil interactions, reducing hardness and weakening textural properties. Further analysis showed that SPI-based and PPI-based HMTP exhibited structural changes upon oil addition, with contrasting directional shifts due to differing degrees of protein-oil interactions. These results emphasize the critical role of protein selection in optimizing oil-containing HMTP formulations, with SPI demonstrating clear advantages in achieving desirable texture due to its superior functional properties. This study provides valuable insights for developing sustainable plant-based meat analogs, particularly those requiring oil incorporation to replicate the textural and structural characteristics of traditional meat products.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140026"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0