International Journal of Biological Macromolecules最新文献

{"title":"Systematic rheological investigation of chitosan-modified magnetite-reinforced thermosensitive Pluronic F127/chitosan hybrid hydrogels.","authors":"V S Aiswarya Gowri, Karolinekersin Enoch, Anbumozhi Angayarkanni Somasundaram","doi":"10.1016/j.ijbiomac.2026.154346","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.154346","url":null,"abstract":"<p><p>Polymer-modified magnetite nanoparticles hold great promise for diverse biomedical applications, including drug delivery, magnetic hyperthermia, and tissue engineering. However, ensuring their long-term functionality and stability for in vivo applications remains a significant challenge. In this work, chitosan-modified magnetite nanoparticles of different concentrations (0-4% w/v) were incorporated into a Pluronic F127/chitosan thermosensitive hydrogel matrix. All hydrogel formulations exhibited a temperature-induced sol-gel transition in the range of 27-28.94 °C, with viscosity increasing with increasing temperature, confirming their thermoresponsive behaviour. Furthermore, all the hydrogels displayed non-Newtonian shear-thinning behaviour. Incorporation of 4% (w/v) chitosan-modified magnetite nanoparticles into the neat hydrogel enhanced the viscosity and yield stress by 28% and 56%, respectively. The storage modulus exceeded the loss modulus for all hydrogels across the oscillatory strain range within the linear viscoelastic region and over the entire frequency range with no crossover points, validating their structural integrity and suitability for long-term in vivo applications. Thixotropic studies revealed excellent structural recovery in these hydrogels upon removal of the applied shear stress. These rheological findings position chitosan-modified, magnetite-incorporated thermoresponsive hydrogels as a structurally resilient and stable system that exhibits both magnetic and thermoresponsive behaviour, making them suitable for multifunctional biomedical applications such as magnetic hyperthermia, targeted drug delivery, and regenerative medicine.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154346"},"PeriodicalIF":8.7,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-temperature-tolerant Cas12a enables instrument-free detection of fungal pathogens at mushroom cultivation temperatures.","authors":"Fan''ge Kong, Yuchong Zhang, Xin Zhang, He Zhang, Yu Li, Jiasi Wang, Di Wang","doi":"10.1016/j.ijbiomac.2026.154352","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.154352","url":null,"abstract":"<p><p>Reliable molecular diagnosis at the point of care remains challenging owning to its dependence on specialized instrumentation and temperature control. However, clustered regularly interspaced short palindromic repeats (CRISPR) and the associated Cas12a protein have shown promise for point-of-care detection. Therefore, an engineered Cas12a variant from Lachnospiraceae bacterium, namely L.b. Cas12a Ultra, was developed. It exhibited markedly enhanced functional stability and low-temperature tolerance, maintaining robust activity across typical mushroom cultivation temperatures (21 °C-29 °C), compared with wild-type LbaCas12a. By combing this low-temperature-tolerance Cas12a with recombinase-aided amplification (RAA), an instrument-free nucleic acid detection system, termed RAA-CUTE, was established targeting the mushroom cobweb disease pathogen Cladobotryum. This system operates within the typical temperature range for mushroom cultivation. Visualization of RAA-CUTE is achieved using lateral-flow assay strips and fluorescent probes. The method shows high specificity and selectivity in complex sample matrices, with a detection limit of 10 fM and minimal background interference. It also effectively discriminates closely related species through specially designed CRISPR crRNAs, as confirmed in validation assays. This single-tube detection platform provides a sensitive, specific, and field-deployable solution for molecular diagnostics in settings with constrained temperature control and instrumentation, making it a promising tool for on-site crop disease surveillance.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154352"},"PeriodicalIF":8.7,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel type III pullulan hydrolase from the bacterium Thermus scotoductus: Biochemical characterization and functional analysis of its carbohydrate-binding domains.","authors":"Yi Gao, Zijian Fu, Lan Xu, Xiaorong Xue, Yazhong Xiao, Hui Peng","doi":"10.1016/j.ijbiomac.2026.154354","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.154354","url":null,"abstract":"<p><p>A novel type III pullulan hydrolase (PulTS) was identified from the thermophilic bacterium Thermus scotoductus, representing the first bacterial member of this enzyme class. The recombinant PulTS exhibited optimal activity at pH 6.5 and 80 °C, with a specific activity of 46.3 ± 2.4 U/mg toward pullulan. The enzyme hydrolyzed pullulan into maltotriose, panose, maltose, and glucose, and also degraded soluble starch, amylose, amylopectin, dextran, glycogen, and γ-cyclodextrin. PulTS contains sequentially arranged carbohydrate-binding module family 48 (CBM48), CBM34, and glycoside hydrolase family 13 subfamily 20 (GH13_20) catalytic domains, an arrangement previously observed only in archaeal species. Deletion of CBM48 enhanced the thermostability of PulTS by approximately 3-fold, yet the enzyme still retained approximately 69% of its catalytic efficiency toward pullulan. Deletion of CBM34 resulted in no further changes. Affinity gel electrophoresis revealed that CBM48 bound pullulan and, more weakly, soluble starch, while CBM34 exhibited only weak binding ability toward pullulan. The results indicated that CBM48 was the major contributor to substrate binding and enzymatic activity, whereas CBM34 played a minor role in PulTS. This work expands our knowledge of pullulan-hydrolyzing enzymes.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154354"},"PeriodicalIF":8.7,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A green and efficient approach for extracting pectin from citrus peels using a ternary deep eutectic solvent: Process optimization, structural characterization, and comparative property evaluation.","authors":"Zhen Wang, Jinshuo Liu, Zonghan Yu, Haojun Lin, Xiangjun Wang, Jia Wu, Weichun Pan, Xiaoxiang Han","doi":"10.1016/j.ijbiomac.2026.154317","DOIUrl":"10.1016/j.ijbiomac.2026.154317","url":null,"abstract":"<p><p>Pectin is a high-molecular-weight polysaccharide widely used in food and pharmaceutical applications. Conventional acid extraction methods are limited by low efficiency and the use of environmentally hazardous reagents. In this study, a novel ternary deep eutectic solvent (DES), composed of choline chloride, malic acid, and ethylene glycol, was developed for efficient pectin extraction from citrus peel powder. Optimal extraction conditions were determined using response surface methodology, yielding a pectin extraction rate of 25.4%. The physicochemical and structural properties of DES-extracted pectin were systematically compared with those obtained via conventional acid extraction. Results showed that DES significantly improved pectin yield. Both samples were identified as high-methoxyl pectins, mainly composed of galacturonic acid, galactose, and arabinose. The DES-extracted pectin exhibited higher antioxidant activity and gel strength, but slightly lower water- and oil-holding capacities and viscosity. These findings demonstrate that ternary DES is a green and efficient alternative for pectin extraction with enhanced functional properties.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154317"},"PeriodicalIF":8.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Electrospun nanofibers based on carboxymethyl cellulose/polyvinyl alcohol as potential antimicrobial wound dressing\" [Int. J. Biol. Macromol. 214 (2022) 111-119].","authors":"Fahimeh Kazeminava, Siamak Javanbakht, Mohammad Nouri, Khosro Adibkia, Mehdi Yousefi, Majid Ahmadi, Pourya Gholizadeh, Hossein Samadi Kafil","doi":"10.1016/j.ijbiomac.2026.154264","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.154264","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154264"},"PeriodicalIF":8.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a cellulose nanofiber-based magnetic hydrous zirconium oxide nanocomposite for phosphate adsorption and struvite recovery.","authors":"Xiaohuan Liu, Enci Hu, Jiafeng Wang, Zongteng Niu, Dan Qiu, Jiayao Yang, Enmin Zong","doi":"10.1016/j.ijbiomac.2026.154342","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.154342","url":null,"abstract":"<p><p>Utilizing sustainable cellulose nanofibers (CNF) as a substrate, this study developed a magnetic nanocomposite for efficient phosphate adsorption and recovery. Through a facile synthetic method, nanoscale ferroferric oxide (Fe₃O₄) and hydrous zirconium oxide (ZrO₂·nH₂O) were anchored onto polyethyleneimine (PEI)-functionalized CNF to fabricate magnetic zirconium-functionalized aminated nanocellulose composites. The optimal composite (CNF-PEI@Fe₃O₄-Zr4) exhibited a high specific surface area and delivered an exceptional phosphate adsorption capacity of 78.13 mg P/g, surpassing some zirconium-based adsorbents and significantly outperforming pristine hydrous zirconium oxide (48.08 mg P/g). Phosphate adsorption followed pseudo-second-order kinetics and the Langmuir isotherm, and the thermodynamic analysis indicated an endothermic and spontaneous process. The composite maintained highly suitable for phosphate removal from wastewater containing high levels of sulfate, nitrate, or chloride. After seven consecutive adsorption-desorption cycles, the removal rate underwent only a marginal decrease, retaining over 96% of its initial efficiency. Furthermore, phosphate was successfully recovered from the spent adsorbent as crystalline struvite (MgNH₄PO₄·6H₂O), as confirmed by XRD, FTIR, and SEM-EDX analyses. The recovered product exhibited characteristic orthorhombic morphology with an Mg:P molar ratio of 0.96:1, and its XRD pattern matched the commercial struvite standard. The adsorption mechanism was attributed to a synergistic effect of electrostatic attraction and ligand exchange. This work presents a facile strategy to transform renewable cellulose into a high-performance adsorbent for advanced phosphate remediation and resource recovery.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154342"},"PeriodicalIF":8.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Development and analysis of de novo transcriptome assemblies of multiple genotypes of Cymbopogon spp. reveal candidate genes involved in the biosynthesis of aromatic monoterpenes\" [Int. J. Biol. Macromol. (2023) 253(Pt 8):127508. doi:10.1016/j.ijbiomac.2023.127508].","authors":"Sheetal Bhat, Arti Sharma, Priyanka Sharma, Kanwaljeet Singh, Maridul Kundan, Mohd Fayaz, Mir Abdul Wajid, Sumeet Gairola, Prashant Misra","doi":"10.1016/j.ijbiomac.2026.154258","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.154258","url":null,"abstract":"","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154258"},"PeriodicalIF":8.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered biodegradable films from derived carbohydrate with dual UV-shielding and antioxidant activity for packaging.","authors":"Sanjoy K Basu, Nirupam Das, Anirban J Banerjee, Rajiv K Kar","doi":"10.1016/j.ijbiomac.2026.154310","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.154310","url":null,"abstract":"<p><p>The environmental crisis triggered by the use of plastic derivatives demands the development of efficient and biodegradable alternatives for packaging materials. The strategy of utilizing agri-waste for developing nanocomposites that can serve as a bioplastic can be valuable. In this study, we report the development of films by incorporating banana pseudo-stem derived carbon quantum dots (CQDs) and nanocellulose (NC) into polyvinyl alcohol/starch/glycerol /carboxymethyl cellulose (CMC) matrix. The CQDs of size 2-3 nm were synthesised via the hydrothermal method, while the NC was extracted through acid hydrolysis exhibiting a crystallinity index of 52%. The films show optimal mechanical and optical properties. Incorporation of CQDs helps achieve a concentration-dependent UV-shielding efficiency up to 97.84% against UV_B and 99.87% against UV_C and prevents photo-oxidative degradation. Additionally, incorporation of CMC results in significant ductility with elongation at break of >101% and antioxidating property with 97.76% radical scavenging rate. We accounted for the real-time packaging efficiency of these films by experimenting on banana via coating and jujube by wrapping as model fruits. Our experiment shows delayed senescence, chlorophyll degradation, and extended shelf-life up to 8 days. Furthermore, the nanocomposite films exhibit nearly 80% biodegradation under natural conditions in soil over 35 days. Overall, the sustainable nanocomposite platform demonstrates potential for packaging application where UV shielding, antioxidant protection, and mechanical flexibility are required.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154310"},"PeriodicalIF":8.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two fructans from Allium macrostemon Bunge alleviate dextran sulfate sodium-induced intestinal injury in Drosophila melanogaster.","authors":"Qiuye Heng, Junhao Li, Qian Zhang, Han Zhao, Zhongnan Wu, Xiaolong Xu, Shaojie Zhang","doi":"10.1016/j.ijbiomac.2026.154197","DOIUrl":"10.1016/j.ijbiomac.2026.154197","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is a chronic inflammatory bowel disease for which current therapies remain limited by incomplete responses, disease relapse, adverse effects, and high treatment costs. Accordingly, the development of safe and effective natural bioactive compounds has attracted increasing interest. Two polysaccharides, designated AMP80-1 and AMP80-2, were isolated and purified from Allium macrostemon Bunge. AMP80-1 (6276 Da) and AMP80-2 (13,168 Da) were determined to be fructans, which were composed of the following sugar residues: β-D-Fruf-(2→, →1)-β-D-Fruf-(2→, →6)-α-D-Glcp-(1→, →6)-β-D-Fruf-(2→, and →1,6)-β-D-Fruf-(2→. Both fructans significantly alleviated intestinal barrier leakage and midgut shortening, suppressed excessive expansion of intestinal stem cells (ISCs) and enteroblasts (EBs), reduced intestinal epithelial cell death and apoptosis-associated DNA fragmentation, and attenuated intestinal oxidative stress. Direct comparisons showed that AMP80-1 exerted significantly greater effects than AMP80-2 on midgut length, 7-aminoactinomycin D (7-AAD) fluorescence, and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) fluorescence, whereas no significant between-group differences were observed in Brilliant Blue accumulation, ISC/EB abundance, or dihydroethidium (DHE) fluorescence. Further reverse transcription quantitative PCR (RT-qPCR) analysis showed that AMP80-1 attenuated dextran sulfate sodium (DSS)-induced increases in p38b, bsk, upd1, upd2, upd3, and Stat92E expression, suggesting an association with attenuated p38/JNK-related epithelial stress and restrained Upd/JAK/STAT-associated regenerative responses. Overall, these findings demonstrate that AMP80-1 and AMP80-2 protect against DSS-induced intestinal injury in D. melanogaster and provide preliminary evidence that molecular size and fine structural differences may influence the intestinal protective activity of A. macrostemon fructans.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154197"},"PeriodicalIF":8.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular RNA Fgfr2 suppresses hepatic fibrosis through the miR-1941-5p/DDAH1 axis and modulates oxidative stress- and autophagy-related signaling.","authors":"Li Gong, Sijia Huang, Bingxin Dai, Pingping Wang, Hailan Qin, Fangting Wu, Gangqiang Xu, Siwei Xia, Weice Sun, Qingyue Hu, Zhenhua Zhang, Zhenzhong Feng, Cuiping Ren, Jijia Shen, Miao Liu","doi":"10.1016/j.ijbiomac.2026.154341","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2026.154341","url":null,"abstract":"<p><p>Hepatic fibrosis is driven by sustained activation of hepatic stellate cells (HSCs), but the post-transcriptional mechanisms that couple pathogen-induced liver injury to HSC activation remain poorly understood. Here, we profiled circular RNAs (circRNAs) in primary HSCs from Schistosoma japonicum-infected mice and identified circFgfr2, a 639-nt circRNA generated from exons 3-6 of Fgfr2, as a fibrosis-associated transcript. CircFgfr2 was reduced in activated HSCs and fibrotic mouse livers. In human liver specimens from mixed non-schistosomal etiologies, reduced circFgfr2 expression was associated with greater fibrosis severity. CircFgfr2 displayed canonical circular features, including a back-splice junction, resistance to RNase R and enhanced transcript stability, and was predominantly cytoplasmic. Mechanistically, the RNA-binding protein Fused in Sarcoma (FUS) promoted circFgfr2 biogenesis by binding the downstream flanking intron of pre-Fgfr2. CircFgfr2 overexpression suppressed HSC activation and proliferation, whereas selective circFgfr2 knockdown increased α-SMA and COL1A1 expression in JS-1 cells. In vivo, early adeno-associated virus serotype 8 (AAV8)-mediated circFgfr2 overexpression attenuated the subsequent development of schistosomiasis-associated hepatic fibrosis. In the murine experimental system, cytoplasmic circFgfr2 acted as a competing endogenous RNA (ceRNA) for mmu-miR-1941-5p, thereby relieving mmu-miR-1941-5p-mediated repression of dimethylarginine dimethylaminohydrolase 1 (DDAH1), reducing intracellular reactive oxygen species (ROS) accumulation, and altering autophagy-related signaling. These findings support an inhibitory role of circFgfr2 in HSC activation and experimental hepatic fibrogenesis and establish a circFgfr2/mmu-miR-1941-5p/DDAH1 regulatory pathway in the murine experimental system. However, the efficacy of circFgfr2 against established fibrosis and conservation of the murine miRNA-mediated mechanism in humans remain to be determined.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"154341"},"PeriodicalIF":8.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}