International Journal of Biological Macromolecules最新文献_第2页

Loading of tea polyphenols into electrospun starch-based nanofibrous films for active package of pre-processed fish fillets. 茶多酚在电纺丝淀粉基纳米纤维膜上的负载，用于预处理鱼片的活性包装。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145370

Jun Wang, Yuxuan Si, Yanan Liu, Xun Zhang, Jingguo Xu, Huiqing Xu, Zhuangli Kang, Lingyan Kong, Songnan Li

{"title":"Loading of tea polyphenols into electrospun starch-based nanofibrous films for active package of pre-processed fish fillets.","authors":"Jun Wang, Yuxuan Si, Yanan Liu, Xun Zhang, Jingguo Xu, Huiqing Xu, Zhuangli Kang, Lingyan Kong, Songnan Li","doi":"10.1016/j.ijbiomac.2025.145370","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145370","url":null,"abstract":"<p><p>This study explores the development of environmentally friendly tea polyphenol (TP)-loaded natural polysaccharide-based nanofibrous films (FFs) using green electrospinning techniques for active packaging of pre-processed fish fillets. Three types of TPs were incorporated into an aqueous octenylsuccinylated starch (OSS)/pullulan (PUL) dispersion to produce TP/OSS/PUL-FFs, whose structural characteristics (appearance, chromaticity, and fiber morphology) and functional properties (antioxidant capacity, antibacterial activity, thermal stability, and water contact properties) were comprehensively evaluated. Among the FFs, TP3/OSS/PUL-FF exhibited the best performance, including the highest thermal degradation temperature (302.04 °C), the lowest water contact angle (17.85°), and the strongest antioxidant and antibacterial properties, attributed to its high epigallocatechin gallate (EGCG) content. When applied to pre-processed fish fillets during an 8-day storage period at 4 °C, the TP3/OSS/PUL-FF active packaging significantly reduced spoilage indicators, including total colony count, pH fluctuation, total volatile basic nitrogen, and thiobarbituric acid reactive substances, outperforming control groups. These findings highlight the potential of TP3/OSS/PUL-FF as an effective and sustainable active packaging material for extending the shelf life of pre-processed meat products.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145370"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae-based bio-fabrication using zinc oxide-chitosan nanocomposite for industrial effluent degradation and pollutant reduction. 氧化锌-壳聚糖纳米复合材料制备微藻降解工业废水及降低污染物。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145379

Karthik Subramani, Naren Vidaarth Tamilselvi Mohanasundaram, Surendhiran Srinivasan, Jagan Krishnaveni Selva Ganeshan, Aran Incharoensakdi

{"title":"Microalgae-based bio-fabrication using zinc oxide-chitosan nanocomposite for industrial effluent degradation and pollutant reduction.","authors":"Karthik Subramani, Naren Vidaarth Tamilselvi Mohanasundaram, Surendhiran Srinivasan, Jagan Krishnaveni Selva Ganeshan, Aran Incharoensakdi","doi":"10.1016/j.ijbiomac.2025.145379","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145379","url":null,"abstract":"<p><p>The current study investigates the photocatalytic degradation of harmful pollutants from the textile and pharmaceutical industries. Specifically, a chitosan zinc nanocomposite's high photocatalytic degradation efficiency was demonstrated. Zinc nanoparticles were synthesized using an extract derived from Chlorella sp. as a reducing agent. XRD analysis revealed a crystal size of 21 nm for the nanocomposite, confirming its hexagonal phase with a wurtzite structure. Particle size analysis determined an average particle size of 38.7 nm for the nanocomposite. The nanocomposite's photocatalytic degradation of the textile pollutant (methyl orange) and the pharmaceutical pollutant (metformin) was evaluated, achieving 98.7 % degradation in 90 min and 98.2 % degradation in 120 min, respectively. Notably, chitosan zinc nanocomposite exhibited maximum degradation efficiencies of 99.1 % and 98.9 %, respectively, against methylene blue and rhodamine B dyes after 105 min, whereas congo red and eosin yellow achieved 96.8 % and 95.5 % degradation efficiencies after 120 min, respectively. Following the degradation process, the photocatalyst's stability and reusability were assessed over seven cycles. Furthermore, the antibacterials and antioxidant activity of the nanocomposite were evaluated, demonstrating superior performance compared to standard agents. These results highlight the multifunctional capabilities of chitosan zinc nanocomposite for environmental remediation and biomedical applications.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145379"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water-soluble β-glucan from G. lucidum as a potential functional food ingredient with gut microbiota-regulating and immune-enhancing activities. 灵芝水溶性β-葡聚糖作为一种潜在的功能性食品成分，具有调节肠道微生物群和增强免疫的作用。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145361

Xue Wang, Linlin Ma, Yuanning Wang, Jialei Xu, Jieyi Long, Xingda Ju, Yunhe Qu, Kevin H Mayo, Yifa Zhou, Lin Sun, Hairong Cheng

{"title":"Water-soluble β-glucan from G. lucidum as a potential functional food ingredient with gut microbiota-regulating and immune-enhancing activities.","authors":"Xue Wang, Linlin Ma, Yuanning Wang, Jialei Xu, Jieyi Long, Xingda Ju, Yunhe Qu, Kevin H Mayo, Yifa Zhou, Lin Sun, Hairong Cheng","doi":"10.1016/j.ijbiomac.2025.145361","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145361","url":null,"abstract":"<p><p>Ganoderma lucidum is a nutraceutical and a traditional medicinal mushroom. β-glucans are the predominant bioactive components in G. lucidum. The molecular weight of β-glucan extracted from G. lucidum by using traditional method is too high, resulting in low solubility and low biological activity. In this study, we obtain a soluble and homogeneous branched β-glucan with (1 → 3)-linked backbone and (1 → 6)-linked side chains (named GLP-D) by using membrane filtration and ultrasonic degradation. GLP-D has simple structures, which is easy for the quality control in future production. GLP-D showed a significant protective effect against cyclophosphamide-induced suppression of lymphocyte proliferation. When gut microbiota was depleted via antibiotic treatment, GLP-D-induced enhancement of splenic lymphocyte proliferation was significantly abrogated. Further, using streptomycin treatment and in vitro fluorescence-activated cell sorting (FACS), we identified Lactobacillus and Alistipes as the bacterium potentially responsible for the effects of GLP-D. GLP-D may be serving as a novel prebiotic which is beneficial for immunocompromised individuals. These findings are crucial to the utilization of G. lucidum.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145361"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Lycium barbarum polysaccharide alleviate cadmium-induced mitochondrial dysfunction mediated pyroptosis in duck liver" [Int. J. Biol. Macromol., volume 311 part 4 (June 2025), 143989]. 枸杞多糖减轻镉诱导的线粒体功能障碍介导的鸭肝脏焦亡[j]。生物。絮凝。， 311卷第4部分（2025年6月），143989]。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.144991

Jie Song, Xianzu Luo, Ling Xiong, Sifan Li, Yan Chen, Wei Liu, Yan Yuan, Yonggang Ma, Jianchun Bian, Zongping Liu, Hui Zou

引用次数: 0

Biotransformation method for corneal tissue engineering: In vitro study of the fabrication and characterization of a decellularized hydrogel from salmon skin extracellular matrix. 角膜组织工程的生物转化方法：鲑鱼皮肤细胞外基质脱细胞水凝胶的制备和表征的体外研究。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145373

Mahshad Kamalvand, Mohammad Sadegh Nourbakhsh, Saeed Heidari Keshel

{"title":"Biotransformation method for corneal tissue engineering: In vitro study of the fabrication and characterization of a decellularized hydrogel from salmon skin extracellular matrix.","authors":"Mahshad Kamalvand, Mohammad Sadegh Nourbakhsh, Saeed Heidari Keshel","doi":"10.1016/j.ijbiomac.2025.145373","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145373","url":null,"abstract":"<p><p>Decellularized marine tissues are ideal for tissue engineering due to their' unique features. In this study, considering the structural similarity of the salmon skin ECM with the human cornea and using the pH sensitivity of biological macromolecules in the fish skin ECM, the irregular structure of salmon skin ECM was transformed into a regular layered structure similar to the human corneal ECM, and its potential as a tissue engineering scaffold was explored. We evaluated decellularization protocols using varying concentrations of TX100 (0.1_0.5 %) to create 3D hydrogels from salmon skin. The decellularized hydrogel membranes were prepared at pH 4 using ascorbic acid solution and modified with EDC/NHS and Riboflavin at varying concentrations (0.01_1%). The samples were evaluated for structural analysis, physical, mechanical, and biological properties. The results showed all membranes demonstrated excellent transparency, and structural analysis revealed a successful transformation from irregular to regular structure in the decellularized hydrogel cross-sections. Increasing crosslinker concentration enhanced the denaturation temperature from 40.5 °C to 43.2 °C, doubled the UTS, and extended the degradation time from 4 days to over a month. The decellularized hydrogel scaffolds showed biocompatibility, strong cell adhesion, supported the viability and proliferation of human corneal epithelial cells, and maintained the epithelial phenotype.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145373"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of egg white protein-whey protein mixtures on the characteristics and quality attributes of rice flour and rice cakes. 蛋清蛋白-乳清蛋白混合物对米粉和年糕特性和品质的影响。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145354

Shang-Cheng Liu, Jin-Zhi Li, Qi Lin, Si-Yuan Zhang

{"title":"Effects of egg white protein-whey protein mixtures on the characteristics and quality attributes of rice flour and rice cakes.","authors":"Shang-Cheng Liu, Jin-Zhi Li, Qi Lin, Si-Yuan Zhang","doi":"10.1016/j.ijbiomac.2025.145354","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145354","url":null,"abstract":"<p><p>Rice cake is a traditional food known for its unique taste and characteristics, primarily composed of rice flour. However, its single-component formulation leads to several textural and eating quality issues, such as rapid aging and hardening, elevated glycemic index, and poor water retention. In this study, whey and egg white proteins (EWPM) were mixed with rice flour and pure starch in different ratios to investigate their impacts on textural properties, rheological behavior, and digestive characteristics of the rice flour as well as their interactions with starch. And then the rice flour with added EWPM was steamed into rice cakes to evaluate its impact on the physicochemical properties and quality attributes of the final product. EWPM promoted the whole starch system to more compact microstructures and crystalline structures (confirmed by scanning electron microscope and X-ray diffraction). Furthermore, the introduction of EWPM diminished the hardness, chewiness, and setback values (1349.67-1082.67 cP), while enhancing water-holding capacity, peak viscosity (2099.67-2658.67 cP), shear resistance, and slowly digestible starch content (32.55 %-40.10 %) of rice cakes. Optimal performance was achieved at mass ratios of 3:2 (egg white protein to whey protein) and 10:1 (rice flour to EWPM), demonstrating the composite's significant potential for preparing high-quality rice cakes.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145354"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

F-box ubiquitin-ligating enzyme ZEITLUPE 1 inhibits the promotion of caffeic acid O-methyltransferase 1 in salt tolerance of apple by the multimeric E3 protein complex SCF^ZTL1-mediated ubiquitination modification and protein degradation. F-box泛素连接酶ZEITLUPE 1通过多聚体E3蛋白复合物scfztl1介导的泛素化修饰和蛋白降解抑制咖啡酸o -甲基转移酶1对苹果耐盐性的促进作用。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145399

Liu Shao, Shuqin Chen, Jiaxiang Liu, Xinjie Yao, Xiaolong Zhou, Yongqing He, Yutong Ju, Tianyu Li, Tingting Yan, Kun Xiao, Weiping Mo, Lin Wang

{"title":"F-box ubiquitin-ligating enzyme ZEITLUPE 1 inhibits the promotion of caffeic acid O-methyltransferase 1 in salt tolerance of apple by the multimeric E3 protein complex SCF<sup>ZTL1</sup>-mediated ubiquitination modification and protein degradation.","authors":"Liu Shao, Shuqin Chen, Jiaxiang Liu, Xinjie Yao, Xiaolong Zhou, Yongqing He, Yutong Ju, Tianyu Li, Tingting Yan, Kun Xiao, Weiping Mo, Lin Wang","doi":"10.1016/j.ijbiomac.2025.145399","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145399","url":null,"abstract":"<p><p>Caffeic acid O-methyltransferase (COMT) is involved in melatonin biosynthesis and regulates salt tolerance in plants, but no COMT homologs have been reported in apple. The post-translational mechanisms through which COMT regulates salt tolerance remain elusive in apple. In this study, we identified a novel protein module MdZTL1-MdCOMT1 regulating melatonin biosynthesis and salt tolerance. MdCOMT1 was a key bioactive melatonin synthetase and exhibited positive biology activity in enhancing salt tolerance of apple. MdCOMT1 could interact with MdZTL1, a F-box ubiquitin-ligating enzyme (E3). Interestingly, MdZTL1 negatively regulated melatonin biosynthesis and salt tolerance by forming the multimeric E3 complex SCFZTL1 to target the ubiquitination of MdCOMT1 and its subsequent degradation in the 26S proteasome. These findings suggest that MdZTL1 inhibits the promotion of MdCOMT1 in salt tolerance, with decreasing melatonin biosynthesis and impairing subsequent ROS scavenging. Our study reveals a ubiquitination-mediated post-translational regulation mechanism in salt tolerance, providing novel insights into the improvement of salt-tolerant apple through molecular design breeding.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145399"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of ultrasound-induced sesamol–soybean peptide composite particles (SSPPs) and SSPP-stabilized Pickering emulsions 超声诱导的芝麻-大豆肽复合颗粒（SSPPs）及其稳定的Pickering乳剂的特性

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145367

Ping Han , Fang Ma , Zhouping Wang , Xiang Zhou , Kai Zheng , Shiling Lu , Jingtao Sun , Juan Dong

{"title":"Characteristics of ultrasound-induced sesamol–soybean peptide composite particles (SSPPs) and SSPP-stabilized Pickering emulsions","authors":"Ping Han , Fang Ma , Zhouping Wang , Xiang Zhou , Kai Zheng , Shiling Lu , Jingtao Sun , Juan Dong","doi":"10.1016/j.ijbiomac.2025.145367","DOIUrl":"10.1016/j.ijbiomac.2025.145367","url":null,"abstract":"<div><div>To improve the properties of soybean peptide-based emulsifiers, non-covalent interactions between sesamol and soybean peptides were induced through ultrasound treatment. Accordingly, sesamol–soybean peptide composite particles (SSPPs) with excellent interfacial properties and antioxidant capacity were prepared to achieve the long-term stabilization of emulsion. In SSPPs, the secondary structure of the peptides was altered (increased β-sheet content) and the surface hydrophobicity was reduced owing to hydrophobic interactions and hydrogen bonding between sesamol and soybean peptides. Moreover, the SSPPs could effectively encapsulate sesamol. With the increase in sesamol concentration, the particle size of SSPPs gradually reduced, while their solubility and antioxidant activity increased. LC-MS/MS revealed that the addition of sesamol led to the generation of small peptides (length: 3–7 amino acids) in SSPPs. The proportion of hydrophobic amino acids at the N- and C-terminals of these peptides was >60 %, and the C-terminus was enriched with basic amino acids, which conferred strong antioxidant capacity to the SSPPs. The SSPPs could be effectively adsorbed and rearranged at the oil–water interface, forming an obvious interfacial barrier. Ultimately, Pickering emulsions stabilized by SSPP-1.5 showed the strongest resistance to droplet aggregation as well as lipid and protein oxidation during 30 days of storage.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145367"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CircSETD3 interrupts the bidirectional positive feedback of ErbB3 and Akt by sponging miR-4667-5p to inhibit colorectal cancer progression and cetuximab resistance CircSETD3通过海绵miR-4667-5p阻断ErbB3和Akt的双向正反馈，抑制结直肠癌的进展和西妥昔单抗耐药性

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145352

Xiaomin Li , Tao Chen , Yujie Cai , Jingtao Zhang , Yuxin Wang , Jianjun Wang , Xueyuan Mao , Liancheng Xu , Dongdong Li , Yu Wang , Xiaoyan Wang

{"title":"CircSETD3 interrupts the bidirectional positive feedback of ErbB3 and Akt by sponging miR-4667-5p to inhibit colorectal cancer progression and cetuximab resistance","authors":"Xiaomin Li , Tao Chen , Yujie Cai , Jingtao Zhang , Yuxin Wang , Jianjun Wang , Xueyuan Mao , Liancheng Xu , Dongdong Li , Yu Wang , Xiaoyan Wang","doi":"10.1016/j.ijbiomac.2025.145352","DOIUrl":"10.1016/j.ijbiomac.2025.145352","url":null,"abstract":"<div><div>Circular RNAs play crucial roles in tumor progression and drug resistance. We previously reported that circSETD3 is downregulated in colorectal cancer (CRC) and correlates with tumor size and metastasis; however, the precise biological functions and underlying the mechanisms of action of circSETD3 in CRC remain unclear. Therefore, in the present study, we aimed to investigate the role of circSETD3 in CRC growth, metastasis, and cetuximab resistance using in vitro and in vivo functional assays. Our results demonstrated that circSETD3 acts as a tumor suppressor in CRC progression and cetuximab resistance. Mechanistically, RNA-seq, FISH, dual-luciferase reporter assays, and ChIP assays revealed that reduced circSETD3 expression in CRC activated ErbB3 and its downstream Akt pathway. Notably, we found that the Akt pathway upregulated ErbB3 transcription via HIF1A, indicating the presence of a novel positive feedback loop between ErbB3 and Akt pathway which reinforces CRC progression and drives cetuximab resistance. Furthermore, circSETD3 deficiency in CRC triggered a feedback loop through the miR-4667-5p–RASA4 axis which was effectively suppressed by exosomal circSETD3 supplementation. Thus, our findings highlight circSETD3 to be a promising therapeutic target for inhibiting CRC progression and overcoming cetuximab resistance.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"318 ","pages":"Article 145352"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STAM2-mediated fine-tuning of PD-L1 secretion via small extracellular vesicles in OSCC. stam2介导的OSCC细胞外小泡分泌PD-L1的微调。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI: 10.1016/j.ijbiomac.2025.145350

Xiao-Le Wang, Hou-Fu Xia, Zhuo-Kun Chen, Kui-Ming Wang, Wei Zhang, Gang Chen

{"title":"STAM2-mediated fine-tuning of PD-L1 secretion via small extracellular vesicles in OSCC.","authors":"Xiao-Le Wang, Hou-Fu Xia, Zhuo-Kun Chen, Kui-Ming Wang, Wei Zhang, Gang Chen","doi":"10.1016/j.ijbiomac.2025.145350","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.145350","url":null,"abstract":"<p><p>The secretion of programmed death ligand 1 (PD-L1) via small extracellular vesicles (sEVs) endows this immune checkpoint protein with diverse and systemic roles. This intercellular communication mediated by sEV PD-L1 not only fosters a pro-tumorigenic niche but also induces systematic immunosuppression. Revealing the intricate mechanisms underlying sEV PD-L1 secretion is essential for understanding tumor pathology and developing targeted therapies. Here, we demonstrated the specialization and collaboration among members of the endosomal sorting complex required for transport-0 (ESCRT-0) family in regulating sEV PD-L1 secretion in oral squamous cell carcinoma (OSCC). Mechanistically, STAM2, not STAM1, directly binds cell surface PD-L1, followed by recruiting HRS to initiate the biogenesis of PD-L1<sup>+</sup> sEVs. Specifically, STAM2 binds the functional domains of PD-L1 and HRS through the VHS and ITAM domains respectively, acting as a molecular bridge between PD-L1 and HRS. Therefore, our study delineated the sequential participation of different ESCRT-0 members during sEV PD-L1 secretion and highlighted the pivotal role of STAM2 in orchestrating this process, providing important insights into diseases with aberrant PD-L1 secretion, such as OSCC.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"145350"},"PeriodicalIF":7.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0