International Journal of Biological Macromolecules最新文献_第2页

Corrigendum to "Dose dependent safety implications and acute intravenous toxicity of aminocellulose-grafted-polycaprolactone coated gelatin nanoparticles in mice" [Int. J. Biol. Macromol. 192 (2021) 1150-1159].

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-12-03 DOI: 10.1016/j.ijbiomac.2024.137885

Rehan Khan, Anas Ahmad, Md Meraj Ansari, Abdullah F Alasmari, Nemat Ali, Mir Tahir Maqbool, Syed Shadab Raza

引用次数: 0

Corrigendum to "Effects of ultrasound combined with pH shift modification on functional and structural properties of peanut proteins" [Int. J. Biol. Macromol. 283 (2024) 137874].

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-30 DOI: 10.1016/j.ijbiomac.2024.138184

Xuan Zhang, Dongmei Yan, Wenjuan Qiu, Siyu Chen, Yu Hu, Jian Jin, Chibuike C Udenigwe

引用次数: 0

Effect of deep eutectic solvents on activity, stability, and selectivity of enzymes: Novel insights and further prospects.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-28 DOI: 10.1016/j.ijbiomac.2024.138148

Duan Zhou, Xuefeng Chen, Gaimiao Li, Mouming Zhao, Daoming Li

{"title":"Effect of deep eutectic solvents on activity, stability, and selectivity of enzymes: Novel insights and further prospects.","authors":"Duan Zhou, Xuefeng Chen, Gaimiao Li, Mouming Zhao, Daoming Li","doi":"10.1016/j.ijbiomac.2024.138148","DOIUrl":"10.1016/j.ijbiomac.2024.138148","url":null,"abstract":"Deep eutectic solvents (DESs) have been extensively concerned since 2008 as reaction media in biocatalysis because of their excellent solvent performances. Here, we try to clarify the effects of DESs on the catalytic properties, structure, and conformation of enzymes. Through comprehensive analysis, it is found that the catalytic properties of enzymes can be designed in different DESs through modulating the hydrogen bond acceptors, hydrogen bond donors, and their molar ratio. Structural changes of different enzymes in various DESs are not always consistent, which may be attributed to the original structure of enzymes, DES composition, and the interactions between enzymes and DESs. Moreover, we try to elucidate how DESs interact with varying amounts of water, and furthermore how water in DES affects the catalytic properties of enzymes. The available researches indicate that proper amount of water can integrate into the network of DESs and strengthen the hydrogen-bonding interactions while excessive water will destroy the integrity of DESs. Water affects the performance of enzymes in two possible ways: 1) affecting enzyme affinity and structure directly; 2) influencing the properties of DESs, thus modulating the efficiency of enzymes. This review paves road for researchers to design DESs with desired properties for specific applications.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138148"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insect chitosan derived from Hermetia illucens larvae suppresses adipogenic signaling and promotes the restoration of gut microbiome balance.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-28 DOI: 10.1016/j.ijbiomac.2024.138168

Eun-Ju Kim, Seok-Hui Lee, Tae Hoon Kim, Jin Lee, Chang-Hyung Choi, Sei-Jung Lee

{"title":"Insect chitosan derived from Hermetia illucens larvae suppresses adipogenic signaling and promotes the restoration of gut microbiome balance.","authors":"Eun-Ju Kim, Seok-Hui Lee, Tae Hoon Kim, Jin Lee, Chang-Hyung Choi, Sei-Jung Lee","doi":"10.1016/j.ijbiomac.2024.138168","DOIUrl":"10.1016/j.ijbiomac.2024.138168","url":null,"abstract":"Chitosan, the deacetylated form of chitin, is considered a valuable source of compounds in the feed and food industries. However, the impact of Hermetia illucens larvae chitosan (HCS) with specific physicochemical characteristics on obesity mediated by lipid accumulation and microbiome dysbiosis has not been fully elucidated. We purified HCS with a low molecular weight (84 kDa), low crystallinity, and a high deacetylation rate, characterizing it through several analytical techniques, including gel permeation chromatography, FT-IR, 1H NMR, FE-SEM, and XRD analysis. HCS effectively inhibited the differentiation of 3T3-L1 preadipocytes by suppressing the production of reactive oxygen species. The adipogenic signaling of preadipocytes, mediated by the phosphorylation of mTOR and PPARγ, which are essential for the expression of fatty acid synthase, was attenuated by HCS. In mouse models fed high-fat diets, the oral administration of HCS prevented changes in white adipose tissue and liver weight and reduced plasma levels of total cholesterol. Additionally, the analysis of the microbiota using 16S rRNA revealed that HCS improved dysbiosis by modulating the composition and abundance of specific bacterial genera, including F. rodentium, L. gasseri, L. reuteri, and L. murinus. These findings highlight the potential of HCS as a candidate for the treatment of obesity-related metabolic diseases.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138168"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-scale structural influence of starch on their interaction of caffeic acid and starch after freeze-thaw: Taking potato starch and lotus seed starch as examples. 淀粉的多尺度结构对冻融后咖啡酸与淀粉相互作用的影响：以马铃薯淀粉和莲子淀粉为例。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-23 DOI: 10.1016/j.ijbiomac.2024.137997

Renjie Zhao, Jia Yao, Chi Li, Qiannan Liu, Wei Liu, Liang Zhang, Zhenzhen Zhang, Ruixuan Zhao, Honghai Hu

{"title":"Multi-scale structural influence of starch on their interaction of caffeic acid and starch after freeze-thaw: Taking potato starch and lotus seed starch as examples.","authors":"Renjie Zhao, Jia Yao, Chi Li, Qiannan Liu, Wei Liu, Liang Zhang, Zhenzhen Zhang, Ruixuan Zhao, Honghai Hu","doi":"10.1016/j.ijbiomac.2024.137997","DOIUrl":"10.1016/j.ijbiomac.2024.137997","url":null,"abstract":"Interactions between polyphenols and starch affect the quality of starch-based products. In this study, we investigated the interaction between caffeic acid (CA) and starches with different structures (lotus seed starch (LSS) and potato starch (PS)) after freeze-thaw, and the effects of CA on the physicochemical properties of starch. The results show that CA has distinct effects on starch, driven by differences in the multiscale structure. LSS exhibited a stronger binding capacity to CA based on complexing index and iodine binding analysis. Hydrogen bonding was identified as the primary driving force between CA and starch chains of LSS and PS supported by Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). After addition of CA, the thermodynamic parameters and swelling power of LSS increased, whereas those of PS decreased, which was attributed to variations in the interactions between the -OH groups of CA and amylopectin/amylose through hydrogen bonding. Rheological, textural, and microstructural analyses revealed that CA decreased the viscoelasticity, gelation ability, textural characteristics of both starch gels, and disrupted the starch gel network, with LSS being the most affected. This study provides a theoretical basis for adjusting the quality of frozen starch-based foods based on the interactions between polyphenols and starch.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137997"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of in vitro pectin fermentation on the human fecal microbiome and O-glycosylation of HT29-MTX cells. 体外果胶发酵对人类粪便微生物群和 HT29-MTX 细胞 O-糖基化的影响

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-23 DOI: 10.1016/j.ijbiomac.2024.137710

Tong Zhao, Sining Liu, Yutong Shuai, Xinyi Zhang, Min Chen, Sijie Pei, Yuxi Duan, Shukai Wang, Yu Lu, Zhongfu Wang, Guiping Gong, Linjuan Huang

{"title":"Influence of in vitro pectin fermentation on the human fecal microbiome and O-glycosylation of HT29-MTX cells.","authors":"Tong Zhao, Sining Liu, Yutong Shuai, Xinyi Zhang, Min Chen, Sijie Pei, Yuxi Duan, Shukai Wang, Yu Lu, Zhongfu Wang, Guiping Gong, Linjuan Huang","doi":"10.1016/j.ijbiomac.2024.137710","DOIUrl":"10.1016/j.ijbiomac.2024.137710","url":null,"abstract":"Pectin is a structurally complex heteropolysaccharide that affects intestinal microorganisms and mucin O-glycans. The present study employed an in vitro model to investigate dynamic changes in microbiota during pectin fermentation. Residual pectin fragments arising from its fermentation were applied to HT29-MTX cells to study the effect of pectin structure on mucin O-glycosylation. Prevotella, Bacteroides, and Parabacteroides were found to preferentially degrade galactose, arabinose, and on the rhamnogalacturonan RG-I side chain region and methyl esterification groups of pectin. Bifidobacterium, Enterococcus, Megamonas, and Dorea metabolized the galacturonic HG region on pectin to produce butyrate. All pectin fragments were found to up-regulate total O-glycans (1.55-2.73 fold) and neutral O-glycans (1.11-1.49 fold) on HT29-MTX mucins. The large HG fragment (81.04 kDa) increased significantly the amount of non-fucosylated glycans (by 2.46-fold); whereas the small HG fragment (16.02 kDa) promoted fucosylated (by 9.25 fold), and especially di-fucosylated O-glycans. Collectively, these results demonstrate that gut microorganisms degrade pectin fragments in the following order of utilization: RG-I, RG-II, and HG. The small fragment of HG improves the expression of fucosylated O-glycans in HT29-MTX cells, mainly owing to an increase in di-fucosylated O-glycans.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137710"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive evolution and mechanism elucidation for ethanol tolerant Saccharomyces cerevisiae used in starch based biorefinery.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-27 DOI: 10.1016/j.ijbiomac.2024.138155

Zhaoxian Xu, Yuanyuan Sha, Muzi Li, Sitong Chen, Jie Li, Boning Ding, Yuwei Zhang, Pingping Li, Kang Yan, Mingjie Jin

{"title":"Adaptive evolution and mechanism elucidation for ethanol tolerant Saccharomyces cerevisiae used in starch based biorefinery.","authors":"Zhaoxian Xu, Yuanyuan Sha, Muzi Li, Sitong Chen, Jie Li, Boning Ding, Yuwei Zhang, Pingping Li, Kang Yan, Mingjie Jin","doi":"10.1016/j.ijbiomac.2024.138155","DOIUrl":"10.1016/j.ijbiomac.2024.138155","url":null,"abstract":"Ethanol tolerant Saccharomyces cerevisiae is compulsory for ethanol production in starch based biorefinery, especially during high-gravity fermentation. In this study, adaptive evolution with increased initial ethanol concentrations as a driving force was harnessed for achieving ethanol tolerant S. cerevisiae. After evolution, an outstanding ethanol tolerant strain was screened, which contributed to significant improvements in glucose consumption and ethanol production in scenarios of 300 g/L initial glucose, high solid loadings (30 wt%, 33 wt%, 35 wt% and 40 wt%) of corn, and high solid loadings (30 wt% and 33 wt%) of cassava, compared with the original strain. Genome re-sequencing was applied for the evolved strain, and 504 sense mutations in 205 genes were detected, among which PAM1 gene was demonstrated related to the elevated ethanol tolerance. In sum, this study provided a practical approach for obtaining ethanol tolerant strain and the identified PAM1 gene enhanced our understanding on ethanol tolerant mechanism, as well as provided a target basis for rational metabolic engineering.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"284 Pt 1","pages":"138155"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalytic transfer hydrogenolysis of lignin derived aromatic ethers over MOF derived porous carbon spheres anchored by Ni species.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-30 DOI: 10.1016/j.ijbiomac.2024.138125

Fei Ge, Haihong Xia, Yanrong Wang, Xiaohui Yang, Jianchun Jiang, Minghao Zhou

{"title":"Catalytic transfer hydrogenolysis of lignin derived aromatic ethers over MOF derived porous carbon spheres anchored by Ni species.","authors":"Fei Ge, Haihong Xia, Yanrong Wang, Xiaohui Yang, Jianchun Jiang, Minghao Zhou","doi":"10.1016/j.ijbiomac.2024.138125","DOIUrl":"10.1016/j.ijbiomac.2024.138125","url":null,"abstract":"The efficient hydrogenolysis of CO ether bonds in lignin is the key for producing bio-oil and high-value chemicals. In this work, we synthesized a series of Ni-MOF-derived porous carbon spheres anchored Ni catalysts (Ni/C-x-T) with different metal/ligand molar ratios and calcination temperatures through solvothermal and carbothermal reduction method, and evaluated their catalytic transfer hydrogenolysis (CTH) performance for lignin model compounds using isopropanol as H-donor. The Ni/C-2-400 catalyst exhibited the excellent CTH performance, affording almost 100 % conversion of 2-phenoxy-1-phenylethanol even at a low reaction temperature of 120 °C. It was worth noting that the further hydrogenation of hydrogenolysis products phenol and ethylbenzene could be controlled by adjusting the reaction conditions, achieving phenol and ethylbenzene as main products at 120 °C, cyclohexanol and ethylbenzene at 140 °C, and cyclohexanol and ethylcyclohexane at 200 °C for 4 h. Based on the characterization results, the high catalytic activity of Ni/C-2-400 was attributed to the good dispersion and small particle size of metal Ni particles. Mechanistic studies showed that the cleavage of CO ether bonds was the main reaction pathway, and high temperature helped accelerate hydrogenolysis and subsequent hydrogenation. Moreover, the Ni/C-2-400 catalyst had good stability and applicability to other model compounds. This work could provide some help for the upgrading of lignin and its derivative.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138125"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the inhibition of non-small cell lung cancer mediated by chitosan-based gene carrier delivering STAT3-shRNA.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-29 DOI: 10.1016/j.ijbiomac.2024.138211

Wangwang Zhang, Leyang Song, Yi Zhou, Jishang Sun, Cuiyao Li, Baoqin Han, Jing Chang, Baosan Han, Ting Wang

{"title":"Study on the inhibition of non-small cell lung cancer mediated by chitosan-based gene carrier delivering STAT3-shRNA.","authors":"Wangwang Zhang, Leyang Song, Yi Zhou, Jishang Sun, Cuiyao Li, Baoqin Han, Jing Chang, Baosan Han, Ting Wang","doi":"10.1016/j.ijbiomac.2024.138211","DOIUrl":"10.1016/j.ijbiomac.2024.138211","url":null,"abstract":"Systemic chemotherapy and radiotherapy often yield poor effect in the postoperative treatment of non-small cell lung cancer (NSCLC) and induce drug resistance. Herein, we proposed a targeted therapeutic approach utilizing gene carrier-mediated specific shRNA method. Firstly, the targeted short hairpin shRNA sequence, designed based on the STAT3 gene sequence, was inserted into the eukaryotic expression vector pGPU6/GFP/Neo to form the recombinant plasmid STAT3-shRNA. Next, a novel gene carrier, Vitamin E Succinate-Chitosan-Histidine (VES-CTS-His, VCH), was synthesized through an acylation reaction. The VCH was combined with pGPU6/GFP/Neo STAT3-shRNA recombinant plasmid by electrostatic interactions to form stable particles. VCH/pDNA, with typical nanoscale dimensions, could accumulate in tumor tissues through the EPR effect and enter tumor cells via endocytosis. VCH exhibited good pH responsiveness and could dissociate in the acidic microenvironment of tumors, thereby releasing the plasmids. Subsequently, the plasmids could downregulate STAT3 expression through RNAi effect. Inhibiting or blocking the expression of the STAT3 gene could significantly enhance the apoptotic induction and growth inhibition effects on NSCLC cells through the PI3K and mTOR signaling pathways, thereby achieving the goal of tumor treatment. This study provides a novel method for the construction of novel non-viral gene carriers and clinical gene-targeted therapy for NSCLC.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138211"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive DFT-based study of the antioxidant properties of monolignols: Mechanism, kinetics, and influence of physiological environments. 基于 DFT 的单木质素抗氧化特性综合研究：机理、动力学和生理环境的影响。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-01 Epub Date: 2024-11-25 DOI: 10.1016/j.ijbiomac.2024.138044

Houssem Boulebd

{"title":"A comprehensive DFT-based study of the antioxidant properties of monolignols: Mechanism, kinetics, and influence of physiological environments.","authors":"Houssem Boulebd","doi":"10.1016/j.ijbiomac.2024.138044","DOIUrl":"10.1016/j.ijbiomac.2024.138044","url":null,"abstract":"Monolignols, p-coumaryl alcohol (CouA), coniferyl alcohol (ConiA), and sinapyl alcohol (SinA), are the fundamental materials for lignin biosynthesis, a major component of lignocellulosic biomass. In the present study, we report a comprehensive analysis of the antioxidant properties of monolignols, using density functional theory (DFT) calculations. Under model physiological conditions, monolignols demonstrated a high hydroperoxyl radical scavenging capacity in polar media, with overall rate constants (koverall) ranging from 5.80 × 106 to 1.15 × 107 M-1 s-1. In contrast, this activity was less pronounced in lipid media, with koverall in the range of 2.66 × 102 to 2.61 × 104 M-1 s-1. The single electron transfer (SET) mechanism was found to play a decisive role in water at physiological pH and under basic conditions, whereas the formal hydrogen transfer (FHT) mechanism was the exclusive pathway in aqueous acid conditions and lipid media. Furthermore, the monolignols ConiA and SinA, demonstrated a strong capacity to chelate Cu(II) and Fe(III) ions in water, with apparent equilibrium constants in the range of 9.21 × 1014 to 5.93 × 1021 M-1 s-1. Their complexes were also found to be highly effective in blocking the reduction of Cu(II)-to-Cu(I) and Fe(III)-to-Fe(II) via the ascorbic acid anion pathway.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138044"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0