International Journal of Biological Macromolecules最新文献_第3页

Comparing solution blow spinning and electrospinning methods to produce collagen and gelatin ultrathin fibers: A review. 比较溶液吹丝法和电纺法生产胶原蛋白和明胶超细纤维：综述。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137806

Murilo Álison Vigilato Rodrigues, Mirella Romanelli Vicente Bertolo, Marilia Marta Horn, Ademar Benévolo Lugão, Luiz Henrique Capparelli Mattoso, Ana Maria de Guzzi Plepis

{"title":"Comparing solution blow spinning and electrospinning methods to produce collagen and gelatin ultrathin fibers: A review.","authors":"Murilo Álison Vigilato Rodrigues, Mirella Romanelli Vicente Bertolo, Marilia Marta Horn, Ademar Benévolo Lugão, Luiz Henrique Capparelli Mattoso, Ana Maria de Guzzi Plepis","doi":"10.1016/j.ijbiomac.2024.137806","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137806","url":null,"abstract":"Ultrathin fibers have been used to design functional nanostructured materials for technological and biomedical applications. Combining the use of renewable and compatible sources with the emerging alternative SBS (solution blow spinning) technique opens new opportunities for material applications. In this review, we introduce the benefits of SBS over the classical electrospinning technique by following studies that use collagen or gelatin. SBS offers distinct advantages over electrospinning in the preparation of ultrathin fibers based on natural proteins, including the absence of high-voltage sources and the possibility of using fewer toxic solvents. Notably, there is also the prospect of using SBS directly in injured tissues, opening new strategies for in situ structure assembly SBS is a suitable approach to produce fibers at the nanoscale that can be tailored to distinct diameters by blending or simply adjusting experimental conditions. The focus on producing collagen or gelatin fibers contributes to designing highly biocompatible mats with potential for promoting cellular growth and implantation, even though their applications can be found also in food packaging, energy, and the environment. Therefore, a comprehensive analysis of the topic is essential to evaluate the current strategies regarding these materials and allow for their expanded production and advanced applications.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137806"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Folic-acid-targeted drug delivery system implementing Angelica gigas polysaccharide: A potential strategy for colorectal cancer treatment. 当归多糖叶酸靶向给药系统：治疗结直肠癌的潜在策略

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137653

Yunfei Ge, Mi-Hye Kwon, Fang Kou, Rajavel Arumugam Uthamapriya, Peng Zhang, Dong-Jin Lee, Ruijuan Yang, Honghui Bao, Subramanian Palanisamy, SangGuan You

{"title":"Folic-acid-targeted drug delivery system implementing Angelica gigas polysaccharide: A potential strategy for colorectal cancer treatment.","authors":"Yunfei Ge, Mi-Hye Kwon, Fang Kou, Rajavel Arumugam Uthamapriya, Peng Zhang, Dong-Jin Lee, Ruijuan Yang, Honghui Bao, Subramanian Palanisamy, SangGuan You","doi":"10.1016/j.ijbiomac.2024.137653","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137653","url":null,"abstract":"The study focuses on the development of folate-targeted conjugates utilizing Angelica gigas polysaccharide (F2) as a drug carrier for colorectal cancer therapy. We synthesized F2-C-5-FU conjugates by linking carboxymethyl-5-fluorouracil (C-5-FU) with folic acid (FA) through ester bonding. The drug release behavior of F2-C-5-FU-FA was pH-dependent, favoring release under alkaline conditions. After 96 h in phosphate buffer (pH 7.4), the conjugate exhibited a cumulative release of 54.7 %, which was higher compared to other pH environments. In vitro, F2-C-5-FU-FA showed enhanced cytotoxicity and increased cellular uptake in folate receptor-positive HCT-116 cells compared to A549 cells. The conjugate also induced G2/M cell cycle arrest and modulated the BAX/BCL-2 mRNA expression ratio through the MAPK and NF-κB signaling pathways. In vivo, F2-C-5-FU-FA increased tumor fluorescence intensity, prolonged drug circulation, and reduced organ toxicity to non-target organs. The treatment promoted cancer cell apoptosis by inhibiting the expression of apoptosis-related proteins. Overall, F2-C-5-FU-FA conjugates demonstrate potential as an effective drug delivery system for targeted colorectal cancer therapy.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137653"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano spray-dried particles of in-situ crosslinked alginate and their toxicological characterisation. 原位交联海藻酸盐纳米喷雾干燥颗粒及其毒理学特征。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137750

Jaka Levanič, Ilja Gasan Osojnik Črnivec, Iza Rozman, Mihaela Skrt, Alja Štern, Bojana Žegura, Nataša Poklar Ulrih

{"title":"Nano spray-dried particles of in-situ crosslinked alginate and their toxicological characterisation.","authors":"Jaka Levanič, Ilja Gasan Osojnik Črnivec, Iza Rozman, Mihaela Skrt, Alja Štern, Bojana Žegura, Nataša Poklar Ulrih","doi":"10.1016/j.ijbiomac.2024.137750","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137750","url":null,"abstract":"The feasibility and technical capacity for producing crosslinked sub-micron gels with a nano spray-dryer were studied with variable pH systems incorporating alginate, pectin, and pullulan. The obtained powders were characterized for their morphology, particle size distribution, and their toxicological safety profile using genotoxicity and cytotoxicity assays. Additionally, quercetin was added to the encapsulation system to study the potential of the system to encapsulate this material. The produced powders exhibited morphologies and particle size distributions characteristic for nano spray-drying. The addition of pullulan and pectin to the feed solutions resulted in a particle size increase, with crosslinked alginate particles having a mean value of 1.43 μm, while particles with added pectin and pullulan had a mean particle size of 1.70 and 1.75 μm, respectively. The inclusion of quercetin proved to be problematic with this encapsulation system. Extremely high degradation rates and extremely low encapsulation efficiencies were observed due to the alkaline pH (~10) of the system that is needed to keep the feed dispersion in a liquid state and prevent premature crosslinking of the alginate. Although pectin and pullulan provided some protection for quercetin in the alkaline dispersion, the absolute quercetin content in the final product remained very low, with a maximum achieved encapsulation efficiency of 2.06 %. The safety profile of most produced powders was favourable, as they did not exhibit any significant cytotoxic and genotoxic activity in the HepG2 cell line, except in the case of Alginate/Pullulan which showed a 43 % decrease in cell viability at 500 μg/ml. Samples where quercetin was added did not show any increased toxicological effect.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137750"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polysaccharide with anticancer activity from Grifola frondosa cultured in industrial wastewater of Agaricus bisporus. 从双孢蘑菇工业废水中提取的具有抗癌活性的多糖。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137489

Tao Pan, Jiafu Huang

{"title":"Polysaccharide with anticancer activity from Grifola frondosa cultured in industrial wastewater of Agaricus bisporus.","authors":"Tao Pan, Jiafu Huang","doi":"10.1016/j.ijbiomac.2024.137489","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137489","url":null,"abstract":"Culture conditions for Grifola frondosa using Agaricus bisporus industrial wastewater as a medium were optimized using Plackett-Burman and Box-Behnken methodologies. Plackett-Burman screening identified culture temperature, shaking speed, and wastewater solubility as the key factors influencing G. frondosa biomass. The Box-Behnken design then established optimal fermentation conditions: 23.7 °C, 202 rpm shaking speed, 10.4 % wastewater solubility, pH 6, 150 mL culture volume in a 250 mL flask, 9-day culture duration, and 10 % inoculation volume. Polysaccharides were isolated and purified from both the mycelia and the culture medium. UV and molecular weight analysis determined that the polysaccharides AIPGF01, NEPGF01, AEPGF01, and NIPGF01 were homogeneous, with molecular weights of 234.7 kDa, 125.2 kDa, 80.0 kDa, and 48.6 kDa, respectively. GC revealed that NIPGF01 and AIPGF01 contained only glucose, while NEPGF01 and AEPGF01 comprised both mannose and glucose in ratios of 12.37:83.69 and 10.06:85.78, respectively. FT-IR confirmed the presence of β-glycosidic linkages in all polysaccharides. NIPGF01 exhibited strong anticancer activity, significantly inhibiting human gastric cancer (MGC80-3) and liver cancer (Hep G2) cells. Apoptosis rates at 50 μg/mL, 200 μg/mL, and 800 μg/mL of NIPGF01 were 38.5 ± 3.5 %, 61.4 ± 4.3 %, and 79.2 ± 5.5 %, respectively. Flow cytometry indicated that NIPGF01 induced apoptosis in MGC80-3 cells by arresting them in the S and G2 phases, leading to a marked reduction in G1 phase cells.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137489"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waste lignocellulosic biomass-derived graphitic carbon encased bimetallic nickel‑palladium oxide nanofibers for efficient organic dye pollutant removal and antibacterial actions. 废弃木质纤维素生物质衍生的石墨碳包裹双金属氧化镍钯纳米纤维，用于高效去除有机染料污染物和抗菌。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137655

AngelaaLincy MariaJoseph, Manunya Okhawilai, Saravanan Rajendran, Prasit Pattananuwat

{"title":"Waste lignocellulosic biomass-derived graphitic carbon encased bimetallic nickel‑palladium oxide nanofibers for efficient organic dye pollutant removal and antibacterial actions.","authors":"AngelaaLincy MariaJoseph, Manunya Okhawilai, Saravanan Rajendran, Prasit Pattananuwat","doi":"10.1016/j.ijbiomac.2024.137655","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137655","url":null,"abstract":"A one-stone-for-three-bird strategy comprising lignocellulose waste management, photocatalytic toxic organic dye degradation, and anti-bacterial activity has been demonstrated using waste coconut coir derived carbon-supported NiO/PdO (NiO/PdO@C) nanocomposite. The formation of interconnected fibrous morphology with intact formation of face-centered cubic NiO and tetragonal PdO within the graphitic carbon shell in NiO/PdO@C was identified from various structural and morphological analyses. Additionally, the elemental mapping and high magnification transmission electron microscopy analyses observed the homogeneous distribution of bimetallic oxides and their complete coverage by multilayered carbon shell. After systematic structural and morphological analyses, the prepared materials were exploited as photocatalysts for the degradation of rhodamine 6G dye. The importance of NiO and PdO heterostructure formation toward overall photocatalytic activity was analyzed by performing catalytic efficiency of individual NiO@C and PdO@C nanostructures and achieving the dye removal efficiencies of 44 % and 34 %, respectively. By integrating NiO and PdO, the electron-hole charge separation was greatly increased while the electron-hole recombination was decreased, and thereby NiO/PdO@C-equipped catalysis degraded 94 % of rhodamine 6G dye within 20 min. Furthermore, similar to photocatalytic activity, the NiO/PdO@C also exhibited exceptional anti-bacterial activity against Klebsiella pneumonia (K. pneumonia), Pseudomonas aeruginosa (P. aeruginosa), and Staphylococcus aureus (S. aureus) bacteria.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137655"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water-in-oil-in-water (W/O/W) emulsions with antioxidant and bacteriostatic capabilities: A preliminary exploration of food preservation films. 具有抗氧化和抑菌能力的油包水（W/O/W）乳液：食品保鲜薄膜初探。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137657

Yue Hu, Ligen Xu, Haoyang Sun, Wei Wu, Yanli Wang, Lizhi Lu, Tao Zeng, Long Sheng, Zhaoxia Cai

{"title":"Water-in-oil-in-water (W/O/W) emulsions with antioxidant and bacteriostatic capabilities: A preliminary exploration of food preservation films.","authors":"Yue Hu, Ligen Xu, Haoyang Sun, Wei Wu, Yanli Wang, Lizhi Lu, Tao Zeng, Long Sheng, Zhaoxia Cai","doi":"10.1016/j.ijbiomac.2024.137657","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137657","url":null,"abstract":"The development of stable water-in-oil-in-water (W/O/W) emulsions for edible preservation coatings and films, utilizing their properties, deserves scientific attention. In this study, oregano essential oil and D‑sodium erythorbate were simultaneously loaded into W/O/W emulsions, and the homogenization conditions of the W/O/W emulsions were optimized. The structure and interactions of gum Arabic (GA) and whey protein isolate (WPI) as the outer phase were analyzed. Stable W1/O/W2 emulsions with excellent antimicrobial and antioxidant activities could be produced under the conditions of GA: WPI at 1:1 and W2: W1/O at 5:5. The diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2'-azinobis-(3-ethylbenzenthiazoline-6-sulphonic acid) (ABTS) radical scavenging rates were 86.35 % and 89.35 %, and the inhibition zone diameters for S. aureus and E. coli were 14.03 ± 0.42 mm and 14.17 ± 0.70 mm, respectively. Finally, the W1/O/W2 emulsions were successfully applied to prepare chitosan-based films. This study has the potential to promote the application of W/O/W emulsions in food preservation, emphasizing the need for advancements for real-world adaptability.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137657"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced activity of enzymes encapsulated in spheres metal azolate framework-7 with defects. 增强封装在有缺陷的偶氮金属框架-7 中的酶的活性。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137689

Cai Cheng, Xiaoliang Guo, Yu Feng, Jie Yu, Shi Huang, Liexiong Zhang, Yu Wu, Linna Shao, Xuehan Xu, Lingling Feng

{"title":"Enhanced activity of enzymes encapsulated in spheres metal azolate framework-7 with defects.","authors":"Cai Cheng, Xiaoliang Guo, Yu Feng, Jie Yu, Shi Huang, Liexiong Zhang, Yu Wu, Linna Shao, Xuehan Xu, Lingling Feng","doi":"10.1016/j.ijbiomac.2024.137689","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137689","url":null,"abstract":"Developing metal-organic frameworks (MOFs) with specific structures is critical for improving the activity of embedded enzymes, and defects may be one of the effective methods. Several methods have been demonstrated to be effective in creating defects in MOFs, including post-synthetic treatments, the use of acid as a modulator, and the use of ordinary or thermally sensitive linkers. However, these methods necessitate the utilization of additional substances. Metal azolate framework-7 (MAF-7) is a kind of MOF that was formed by the coordination of Zn2+ with 3-methyl-1,2,4-triazole (Hmtz). This paper presents a method for the preparation of defect MAF-7 by changing the sequence of reactants without the introduction of additional substances. The defects were characterized by a range of techniques, including scanning electron microscopy, transmission electron microscopy, Brunauer-Emmett-Teller, X-ray photoelectron spectroscopy, and powder X-ray diffraction. The activity of microcystinase A (MlrA) encapsulated in defective MAF-7 (CMlrA@DMAF-7) was found to be significantly increased in comparison to non-porous MAF-7 (NMAF-7), and was largely unaffected by alterations in synthesis conditions. It is also noteworthy that lysozyme (LZ) and horseradish peroxidase (HRP), which are commonly used in industry, also demonstrated enhanced activity when encapsulated in DMAF-7. It was therefore anticipated that modifying the sequence of reactant addition would be a straightforward and simple method of introducing defects into MAF-7, thereby improving enzyme utilization.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137689"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functionalisation and behaviours of polysaccharides conjugated with phenolic compounds by oxidoreductase catalysis: A review. 通过氧化还原酶催化与酚类化合物共轭的多糖的功能化和行为：综述。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137660

Koceila Boundaoui, Didier Le Cerf, Virginie Dulong

{"title":"Functionalisation and behaviours of polysaccharides conjugated with phenolic compounds by oxidoreductase catalysis: A review.","authors":"Koceila Boundaoui, Didier Le Cerf, Virginie Dulong","doi":"10.1016/j.ijbiomac.2024.137660","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137660","url":null,"abstract":"Polysaccharides have been extensively studied in recent decades. Their conjugation with phenolic compounds of natural origin has demonstrated high added value, not only enhancing certain inherent properties but also introducing new ones. Recently, a sustainable approach utilizing oxidoreductase enzymes (Oxredases) (laccases (Lac), tyrosinases (Tyr) and peroxidases (Per)) has been employed, and interesting progress has been made. This review aims to provide a comprehensive understanding of the various Oxredases employed and the reaction mechanisms involved in the grafting of phenolic compounds (PCs) onto polysaccharides (PSs). We provide a detailed analysis of these reaction mechanisms based on modified polymer structural analysis techniques and supported by model reactions. The impacts of different reaction parameters on the final products are thoroughly discussed. Additionally, the properties of conjugated PS-PC are extensively explored to provide an overview of their physicochemical and functional behaviours. A comparison of the PS-PC conjugates obtained via the Oxredase approach and other existing chemical- and radical-mediated approaches is also presented, emphasizing the benefits of this enzymatic pathway. Finally, a critical analysis is proposed to identify areas of improvement, aiming to further refine this environmentally friendly approach for conjugating PSs and PCs and its scalability for industrial applications.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137660"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of three-dimensional whey protein amyloid fibril-based hybrid aerogels in oil/water separation and emulsion separation. 基于三维乳清蛋白淀粉样纤维的混合气凝胶在油/水分离和乳液分离中的应用。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137680

Jia-Ling Tu, You-Ren Lai, Chia-Yu Lin, Steven S-S Wang, Ta-Hsien Lin

{"title":"Applications of three-dimensional whey protein amyloid fibril-based hybrid aerogels in oil/water separation and emulsion separation.","authors":"Jia-Ling Tu, You-Ren Lai, Chia-Yu Lin, Steven S-S Wang, Ta-Hsien Lin","doi":"10.1016/j.ijbiomac.2024.137680","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137680","url":null,"abstract":"Environmental contamination from oil spills and industrial wastewater poses long-term risks to ecosystems and human health. Amyloid fibrils' superior stiffness and stability, outstanding biocompatibility and biodegradability, versatile functional groups, and high specific surface area make them promising sustainable adsorbents. This study is aimed at examining the application of three-dimensional polysaccharide-modified whey protein amyloid fibril (WPIAF) aerogels in oil/water separation and emulsion separation. The WPIAF aerogel was first synthesized through the salting out method coupled with lyophilization, then modified using carboxymethyl cellulose (CMC) or chitosan (CS). Our results showed that WPIAF aerogels exhibited increased mechanical properties and surface hydrophobicity after polysaccharide modification. The CS-modified WPIAF aerogels showed better oil adsorption capacities, oil adsorption capability, and reusability than WPIAF aerogel and CMC-modified WPIAF aerogels. Furthermore, the applicability of polysaccharide-modified WPIAF aerogels in a continuous oil/water separation system was demonstrated. Finally, the separation efficiencies of polysaccharide-modified WPIAF aerogels were determined to be over 90 % in various emulsion systems, and the possible separation mechanism was further investigated. This study provides a nice example of applying amyloid-based aerogels for oil/water separation and emulsion separation.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137680"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan microcarriers loaded with functional drug for stimulating osteogenesis and angiogenesis in vitro. 载入功能性药物的壳聚糖微载体用于体外刺激成骨和血管生成。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2024-11-17 DOI: 10.1016/j.ijbiomac.2024.137598

Ruirui Kong, Jing Chen, Feilong Zhao, Yan Li, Huiyi Yang, Yudong Zheng, Wei He

{"title":"Chitosan microcarriers loaded with functional drug for stimulating osteogenesis and angiogenesis in vitro.","authors":"Ruirui Kong, Jing Chen, Feilong Zhao, Yan Li, Huiyi Yang, Yudong Zheng, Wei He","doi":"10.1016/j.ijbiomac.2024.137598","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137598","url":null,"abstract":"Angiogenesis-osteogenesis coupling plays important roles in bone regeneration; therefore, biomaterials capable of stimulating both osteogenesis and angiogenesis show significant influence in bone repair. Herein, chitosan (CS) microcarriers loaded with functional drug dimethyloxalylglycine (DMOG) were prepared using the emulsion phase separation and impregnation method for stimulating osteogenesis and angiogenesis. FTIR and zeta potential analyses confirmed successful DMOG loading into CS microcarriers, primarily through physical adsorption, particularly hydrogen-bond interaction. As the impregnation concentration of DMOG increased, the amounts of DMOG loaded into the microcarriers increased, while the drug encapsulation efficiency decreased. All microcarriers, ranging in size from 200 to 400 μm, revealed quasi-spherical shapes and an interconnected porous structure with pore sizes mainly between 15 and 30 μm, suitable for cell attachment and proliferation. The introduction of DMOG increased the residues of the microcarriers during thermogravimetric analysis. CS/DMOG microcarriers exhibited sustained drug release (for >19 days) and good degradation ability. Furthermore, CS/DMOG microcarriers supported stem cell adhesion and proliferation. They also enhanced stem cell osteogenesis verified by strengthening alkaline phosphatase expression and mineralization. Moreover, they promoted angiogenesis, as evidenced by stimulating endothelial cell migration and tube formation. These results suggest that CS/DMOG microcarriers have the potential to be used for bone tissue regeneration.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137598"},"PeriodicalIF":7.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0