International Journal of Biological Macromolecules最新文献_第3页

Decorating channel walls in ordered macroporous ZIF-8 with hydrophilic PEG to immobilize lipase for efficient chiral resolution.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140117

Hao Li, Bizhu Sun, Meiai Huang, Yipeng Liu, Qian Zhang, Zhuolin Luo, Quan Zeng, Wenjing Zhu, Xuan Li, Juan Chen, Xin Yuan, Panliang Zhang, Kewen Tang

{"title":"Decorating channel walls in ordered macroporous ZIF-8 with hydrophilic PEG to immobilize lipase for efficient chiral resolution.","authors":"Hao Li, Bizhu Sun, Meiai Huang, Yipeng Liu, Qian Zhang, Zhuolin Luo, Quan Zeng, Wenjing Zhu, Xuan Li, Juan Chen, Xin Yuan, Panliang Zhang, Kewen Tang","doi":"10.1016/j.ijbiomac.2025.140117","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140117","url":null,"abstract":"The development of efficient immobilization support for the enhancement of enzyme activity and recyclability is a highly desirable objective. Single-crystalline ordered macro-microporous ZIF-8 (SOM-ZIF-8), has emerged as a highly effective matrix for enzyme immobilization, however, the inherent hydrophobic nature limits its further advancement. Herein, we have customized the immobilization of the Pseudomonas cepacia lipase (LP) in the modification-channels of SOM-ZIF-8 by functionalizing the inner surface-properties with polyethylene glycol (PEG) (LP@SOM-ZIF-8-PEG), and significant enhancement of the activity and (thermal, solvent and cyclic) stability can be realized. The incorporation of PEG into SOM-ZIF-8 regulates its inner surface charge and hydrophobic properties, thereby enhancing enzyme loading, facilitating enzyme conformational adjustments, and achieving a uniform dispersion of LP in SOM-ZIF-8-PEG. LP@SOM-ZIF-8-PEG not only demonstrates a pronounced elevation in enzyme loading and activity over LP@SOM-ZIF-8 but also shows an enzyme activity that is impressively three times greater than LP@ZIF-8. It can completely resolve the 1-phenylethanol racemate in 60 min, with a conversion close to 50 % and an enantioselectivity of 99.8 %. After nine cycles of reuse, the LP@SOM-ZIF-8-PEG still holds onto 95 % of its initial activity. The excellent catalytic performance and stability of LP@SOM-ZIF-8-PEG, along with the universality of the PEG modification strategy for other enzymes, make this work promising in industrial applications.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140117"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and application of a time-temperature hydroxyethyl cellulose ink based on Clitoria ternatea L. blue petals anthocyanin and N-Hydroxyphthalimide for food freshness monitoring.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140060

Anning Song, Yanglin Wu, Chunwei Li

引用次数: 0

Effect of oat β-glucan on the freezing resistance of yeast and the underlying mechanism.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140105

Zhen Li, Shengxin Ji, Jie Cai, Biao Suo, Yunhao Zhu, Zhilu Ai

{"title":"Effect of oat β-glucan on the freezing resistance of yeast and the underlying mechanism.","authors":"Zhen Li, Shengxin Ji, Jie Cai, Biao Suo, Yunhao Zhu, Zhilu Ai","doi":"10.1016/j.ijbiomac.2025.140105","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140105","url":null,"abstract":"The objective of this study was to investigate the protective effects of oat β-glucan (OβG) on yeast subjected to freeze-thaw cycle-induced stress. A range of analytical techniques were employed to identify the underlying molecular mechanisms, including flow cytometry, gas chromatography-mass spectrometry, and quantitative real-time PCR. Following three freeze-thaw cycles, the survival rate of yeast that had been supplemented with 0.5 % OβG was found to be significantly higher than that of the control sample, increasing from 36.21 % to 56.81 %. The addition of 0.5 % OβG resulted in a remarkable reduction in apoptosis, an improvement in cell membrane integrity, and an increase in superoxide dismutase, catalase activity and glutathione content compared to the control group. Furthermore, a noticeable increment in the intracellular trehalose content was observed, from 4.10 mg/g to 7.48 mg/g. OβG modulated the expression of trehalose metabolism-related genes (ATH1, NTH1, NTH2) throughout the freeze-thaw cycle. Therefore, it could be concluded that OβG protected yeast cells against excessive reactive oxygen species and minimised oxidative damage to cellular membranes by upregulating antioxidant enzyme activity and total antioxidant capacity. Moreover, the supplementation of OβG was also found to be effective in increasing intracellular trehalose levels, thereby enhancing the freezing resistance of yeast cells.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140105"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multipurpose packaging system based on intelligent carboxymethyl cellulose film and activated cellulose acetate electrospun nanofibers for seafoods.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140115

Hossein Ahangari, Alireza Ebrahimi, Ali Ehsani, Sajed Amjadi

{"title":"Multipurpose packaging system based on intelligent carboxymethyl cellulose film and activated cellulose acetate electrospun nanofibers for seafoods.","authors":"Hossein Ahangari, Alireza Ebrahimi, Ali Ehsani, Sajed Amjadi","doi":"10.1016/j.ijbiomac.2025.140115","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140115","url":null,"abstract":"The objective of this research is to develop a natural macromolecules-based smart double-layer film using carboxymethyl cellulose (CMC) film containing pomegranate peel anthocyanins (PPA) and cellulose acetate nanofibers (CANFs) with Artemisia sieberi Besser essential oil-loaded nanostructured lipid carriers (ABNLCs). Based on the performance as a color indicator, and other studied properties, the CMC/PPA 8 % film was selected as the optimized film. The double-layer film was constructed by electrospinning the CANFs containing ABNLCs on the optimized CMC film. The average diameter of the CANFs was 332.87 ± 65.80 nm. Furthermore, the XRD patterns of CA/ABNLCs NFs showed many diffraction peaks that the sharp peaks at 2θ of 25° and 28°, indicating its crystalline structure. In addition, the Young's modulus and elongation at break point values of the CMC/PPA 8 % double-layered film were reached to 151.47 MPa and 11.39 %, respectively. The intelligent layer demonstrated acceptable performance in detecting the freshness of shrimp and fish fillets. The double-layered film showed good antibacterial activity with inhibition zones of 10 and 12 mm against E. coli and S. aureus pathogens, respectively.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140115"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astragalus polysaccharide reduces the severity of acute pancreatitis under a high-fat diet through enriching L. reuteri and propionate.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.140021

Qiang Wang, Xuxu Liu, Zhiwei Du, Yi Zheng, Ziang Meng, Zhenyi Lv, Liyi Wang, Dongbo Xue

{"title":"Astragalus polysaccharide reduces the severity of acute pancreatitis under a high-fat diet through enriching L. reuteri and propionate.","authors":"Qiang Wang, Xuxu Liu, Zhiwei Du, Yi Zheng, Ziang Meng, Zhenyi Lv, Liyi Wang, Dongbo Xue","doi":"10.1016/j.ijbiomac.2025.140021","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140021","url":null,"abstract":"Acute pancreatitis (AP) is a severe digestive disorder, worsened by a high-fat diet (HFD) through inflammation and gut microbiota disruption. Astragalus polysaccharides (APS), known for their anti-inflammatory properties, may alleviate HFD-induced exacerbation of AP by modulating gut microbiota. This study investigates the effect of APS on AP severity under a HFD (HAP). Results show that HFD significantly worsens AP, with elevated serum enzymes, pro-inflammatory cytokines, and pancreatic damage. Single-cell RNA sequencing revealed increased ICAM1+ neutrophils and activation of the NF-κB/necroptosis pathway in HAP mice. Treatment with APS reduced neutrophil infiltration, downregulated NF-κB, and suppressed necroptosis. APS also restored gut microbiota balance, boosting Lactobacillus reuteri (L. reuteri) and propionate (PA) levels. Interventions with L. reuteri or PA reduced HAP severity, with combined treatment showing synergistic effects. These findings suggest that the protective effect of APS is mediated by microbiota-dependent mechanisms, highlighting the gut-pancreas axis as a potential therapeutic target for AP.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140021"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in isolation, structures, and bioactivities of Ficus carica L. polysaccharides: A review.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.139887

Changwei Wu, Binbin Liao, Chaohai Li, Zemei Zhu, Baozhong Duan, Anzhong Peng, Fumei He

{"title":"Recent advances in isolation, structures, and bioactivities of Ficus carica L. polysaccharides: A review.","authors":"Changwei Wu, Binbin Liao, Chaohai Li, Zemei Zhu, Baozhong Duan, Anzhong Peng, Fumei He","doi":"10.1016/j.ijbiomac.2025.139887","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.139887","url":null,"abstract":"Ficus carica (F. carica) is a well-known medicinal and edible plant with high nutritive values. Polysaccharides are the primary and important bioactive components in F. carica, offering various health benefits. Recently, F. carica polysaccharides (FCPs) have attracted considerable attention due to their various activities, such as anti-oxidant, immunomodulatory, anti-tumor, and anti-diabetic properties. Despite advances in the isolation and characterization of FCPs, critical gaps remain in understanding their structure-activity relationships (SARs), safety concerns, and applications, hindering their full utilization and development. Therefore, a comprehensive review of the latest developments in FCPs is of great significance. Herein, we reviewed the extraction and purification methods, structural characterization, biological activities, SARs, safety concerns, and applications of FCPs. FCPs are water-soluble polysaccharides with molecular weights (Mw) ranging from 21.9 to 6890 kDa, primarily composed of glucose, galactose, arabinose, rhamnose, mannose, and xylose. Factors such as the Mw, monosaccharide composition, and extraction methods are crucial in pharmacological activities. Additionally, current challenges and prospects of FCPs are discussed, aiming to provide valuable insights for future research and clinical translation. In conclusion, this article provides insights and foundational knowledge for further research aimed at applying the therapeutic and multifunctional biomaterial properties of FCPs.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139887"},"PeriodicalIF":7.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Facile degradation of chitosan-sodium alginate-chromium (III) gel in relation to leather re-tanning and filling" [International Journal of Biological Macromolecules, 240 (2023) 124437].

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-19 DOI: 10.1016/j.ijbiomac.2025.139961

Xiaoyu Guan, Bingyuan Zhang, Shiyong Liu, Meng An, Qingxin Han, Dongping Li, Ping Rao

引用次数: 0

UV responded polyvinyl alcohol bio-active films containing oregano essential oil microcapsules by chitosan-incorporated TiO₂: Physical properties, release characterizations, bio-functional performances and applications.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-18 DOI: 10.1016/j.ijbiomac.2025.140049

Lina Xu, Xinglian Xu, Yayan Mao, Yujuan Xu, Mingyuan Huang

{"title":"UV responded polyvinyl alcohol bio-active films containing oregano essential oil microcapsules by chitosan-incorporated TiO2: Physical properties, release characterizations, bio-functional performances and applications.","authors":"Lina Xu, Xinglian Xu, Yayan Mao, Yujuan Xu, Mingyuan Huang","doi":"10.1016/j.ijbiomac.2025.140049","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140049","url":null,"abstract":"In the present study, with oregano essential oil (OEO) as the active ingredient and polyvinyl alcohol/citric acid (PVA/CA) as the composite matrix, ultraviolet (UV) responded PVA bio-active films incorporated with microcapsules, which were established by chitosan-incorporated titanium dioxide (TiO2), were constructed. The UV light-triggered process changed the structure of films, including the degradation of PVA, the fracture of ester bonds and the breaking of glycosidic bonds. UV irradiation reduced the elongation at break, increased the light resistance ability, the surface hydrophobicity and the roughness, and accelerated the release of OEO in films. The release process of films in food simulations and air environment conformed to Fick's second law and the first-order dynamics model, which were attributed to the synergistic effects of the random diffusion and the structural relaxation that were promoted by UV irradiation, leading to the increase of release rate, antioxidant activities and antibacterial capacities of films. Finally, chicken thighs with UV responded PVA bio-active films had better quality and the films could be considered as active packages for preservation.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140049"},"PeriodicalIF":7.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of sugar-based natural framework as phytopathogenic virus capsid protein inhibitors using a state-of-the-art multiple screening strategy.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-18 DOI: 10.1016/j.ijbiomac.2025.140075

Jin-Hong Hu, Zhen-Xing Li, Yue Ding, Yi-Ke Yang, Tai-Hong Zhang, Li-Wei Liu, Xiang Zhou, Song Yang

{"title":"Discovery of sugar-based natural framework as phytopathogenic virus capsid protein inhibitors using a state-of-the-art multiple screening strategy.","authors":"Jin-Hong Hu, Zhen-Xing Li, Yue Ding, Yi-Ke Yang, Tai-Hong Zhang, Li-Wei Liu, Xiang Zhou, Song Yang","doi":"10.1016/j.ijbiomac.2025.140075","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140075","url":null,"abstract":"The prompt and efficient identification of targeted inhibitors against unscrupulous pathogenic viruses holds promise for preventing epidemic disease outbreaks. Herein, a comprehensive multichannel screening method (multiple docking cross-validation, molecular dynamics simulation, and density functional theory calculation) integrated with bioactivity identification is rationally established using sugar-based natural ligand libraries to target tobacco mosaic virus (TMV) capsid proteins. Encouragingly, compounds A0 (Kd = 0.14 μM) and A4 (Kd = 1.43 μM) were evaluated to have excellent binding capacities to TMV capsid protein, evidently exceeding that of viricide Ningnanmycin (Kd = 3.47 μM) by 24.8 and 2.4-folds. Moreover, A0 and A4 significantly down-regulated the expression of capsid proteins at the transcriptional level, effectively blocking the biosynthesis and assembly of TMV in tobacco. Additionally, bioactivity evaluation illustrated that the anti-TMV curative effects of A0 (EC50 = 310.9 μg/mL) and A4 (EC50 = 371.2 μg/mL) were comparable to Ningnanmycin (EC50 = 343.8 μg/mL). Considering the availability, cost and synthesis difficulty of precursors, the more affordable A4 is reckoned to be a promising candidate for capsid protein inhibitors and warrants further exploration in follow-up studies. Current findings highlight that this state-of-the-art virtual strategy, integrated with bioactivity validation, facilitates the discovery of targeted candidates to combat pathogenic viruses.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"298 ","pages":"140075"},"PeriodicalIF":7.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction, purification, characteristics, bioactivities, application, and toxicity of Gastrodia R. Br. polysaccharides: A review.

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-01-18 DOI: 10.1016/j.ijbiomac.2025.140084

Feiya Zhao, Chenglong Yin, Yufeng Lai, Haiming Lin, Zhiming Jian, Aien Tao

{"title":"Extraction, purification, characteristics, bioactivities, application, and toxicity of Gastrodia R. Br. polysaccharides: A review.","authors":"Feiya Zhao, Chenglong Yin, Yufeng Lai, Haiming Lin, Zhiming Jian, Aien Tao","doi":"10.1016/j.ijbiomac.2025.140084","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2025.140084","url":null,"abstract":"Gastrodia elata BL. is a dried tuber of the orchid plant Gastrodia R. Br., mainly distributed in Jilin, Sichuan, Guizhou, Yunnan, and other places, with wind quenching antispasmodic, suppressing the liver and Yang and other efficacy, is one of China's traditional Chinese medicinal herbs. Polysaccharides are the main bioactive substances of Gastrodia R. Br., and they have attracted extensive attention of researchers both at home and abroad. In the past few decades, various methods (such as hot water extraction, ultrasonic extraction, enzymatic extraction, etc) have been applied to extract polysaccharides from Gastrodia R. Br. (GRPs) and the structural features of GRPs are closely related to the methods of extraction and purification. Many studies have confirmed that GRPs possess various remarkable bioactivities, including anti-oxidation, anti-tumor, immune regulation, anti-aging, improving cerebral ischemia, improving memory, lowering blood pressure and glucose, anti-bacterial activities, etc. GRP, as a natural polysaccharide, is widely used in the fields of medicine, food, and health products, and has important research and development value. However, the intrinsic relationship and mechanism of action between its advanced structure and pharmacological effects need further in-depth study. This paper systematically reviews the research on the extraction and purification, structural characteristics, biological activity, modification, and potential applications of GRPs in recent years, providing a theoretical basis for further studying the structure-activity relationship of GRPs and their applications as therapeutic agents and functional foods.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"140084"},"PeriodicalIF":7.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0