Prosecco grape pomace: a new feedstock for high molecular weight hyaluronic acid

IF 8.5 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-10-09 DOI:10.1016/j.ijbiomac.2025.148203

Irene Gugel , Filippo Marchetti , Ilenia Gugel , Giulia Scoponi , Nicola Marchetti , Nada Baraldo , Anna Baldisserotto , Stefania Costa , Stefano Manfredini , Marco Scoponi , Silvia Vertuani

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Abstract

Due to its remarkable properties, hyaluronic acid (HA) has always attracted the attention of academia and industry. However, to meet the growing demand for HA, reduce costs and adopt sustainable approaches lignocellulosic raw materials were investigated as potential low-cost bio-resources. For example, the wine industry provides waste that can be valorised as fermentation substrates. In this study, grape pomace from Prosecco vinification underwent a three-step pre-treatment, resulting in the recovery of approximately 30 g of sugars per 100 g of dry matrix.

The resulting sugar-rich medium was used for the growth of Streptococcus equi subsp. zooepidemicus ATCC 39920, which produced a hyaluronic acid titre of 0.41 g/L after 11 h of batch fermentation. Although derived from a waste matrix, the hyaluronic acid obtained exhibited a lower polydispersity index and a higher molecular weight than a commercially available high molecular weight hyaluronic acid. Therefore, this study demonstrates the feasibility of using lignocellulosic raw materials to produce high-quality hyaluronic acid, while promoting a biorefinery approach to improve the sustainability of wineries.

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普罗赛克葡萄渣：制备高分子量透明质酸的新原料

透明质酸（HA）由于其优异的性能，一直受到学术界和工业界的关注。然而，为了满足对透明质酸日益增长的需求，降低成本并采取可持续发展的方法，木质纤维素原料被研究为潜在的低成本生物资源。例如，葡萄酒行业提供的废物可以作为发酵底物。在这项研究中，普罗赛克酿造过程中的葡萄渣经过了三步预处理，每100克干燥基质中回收了大约30克糖。所得富糖培养基用于马链球菌亚种的生长。ATCC 39920动物流行病菌，批量发酵11 h后产生的透明质酸滴度为0.41 g/L。虽然从废弃基质中提取，但与市售的高分子量透明质酸相比，所获得的透明质酸表现出较低的多分散指数和较高的分子量。因此，本研究证明了使用木质纤维素原料生产高质量透明质酸的可行性，同时促进生物精炼方法提高酿酒厂的可持续性。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.