A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate.

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-12-01 Epub Date: 2025-05-25 DOI:10.1016/j.talanta.2025.128384

Zhichao Zhang, Zefeng Li, Ruen Xie, Zhen Tian, Yicun Yao, Bingwu Yang, Jingao Zhang, Haili Ma, Lan Rao, Kuiru Wang, Binbin Yan, Xinzhu Sang, Chongxiu Yu, Bo Fu, Shengli Pu, Jinhui Yuan, Qiang Wu

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引用次数: 0

Abstract

P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05-5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.

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用于快速检测293T细胞裂解液中p53蛋白的级联锥形七芯纤维生物传感器。

P53蛋白是主要的癌症生物标志物之一，其检测对于乳腺癌、直肠癌、肺癌等多种癌症的早期诊断和治疗具有重要意义。本文提出了一种用于p53蛋白快速检测的级联锥形七芯纤维（CTSCF）生物传感器。实验结果表明，所构建的CTSCF生物传感器可有效检测浓度为0.05 ~ 5 ng/mL的p53蛋白，检出限（LoD）低至0.0496 ng/mL。此外，验证了CTSCF生物传感器检测293T细胞裂解液中p53蛋白的能力，并与传统的western blotting （WB）方法进行了比较。结果表明，CTSCF生物传感器的LoD远低于WB方法。所提出的CTSCF生物传感器具有检测时间快、稳定性和特异性好、灵敏度高、LoD低等特点，在临床医学和病理生理学方面具有很大的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.