Talanta最新文献

{"title":"Immunoelectroanalytical multiplexing of RNA methylation signatures to decode oncogenic point mutations in cancer cells.","authors":"Víctor Pérez-Ginés, Rebeca M Torrente-Rodríguez, Raquel Rejas-González, Ana Montero-Calle, María Pedrero, José M Pingarrón, Rodrigo Barderas, Susana Campuzano","doi":"10.1016/j.talanta.2026.130180","DOIUrl":"10.1016/j.talanta.2026.130180","url":null,"abstract":"<p><p>Epigenomic and epitranscriptomic alterations-particularly RNA methylations such as N⁶-methyladenosine (m6A), N⁵-methylcytosine (m5C), N¹-methyladenosine (m1A), and N⁷-methylguanosine (m7G)-are increasingly recognized as pivotal regulators in the pathobiology of colorectal cancer (CRC) and other highly prevalent disorders. Despite their growing clinical and biological relevance, rapid and robust electrochemical strategies enabling the single (m1A, m7G) or multiplexed quantification of these RNA modifications remain unavailable. Herein, we report an indirect competitive, four-analyte amperometric immunoassay that enables the simultaneous determination of m6A, m5C, m1A, and m7G. BSA-conjugated methylated synthetic nucleotides covalently attached to magnetic carboxylated microsized-beads (HOOC-MμBs) competed with freely methylated nucleotides in the target sample for specific anti-epimark detection antibodies. Thereafter, labeling with horseradish peroxidase (HRP)-conjugated secondary antibodies (Ab2-HRP) enabled amperometric readout at screen-printed carbon electrodes (SPCEs) for single or multiplexed transduction. The developed bioplatforms exhibited high selectivity, negligible cross-interference, and a significantly reduced assay time (30 min), supporting their integration into a robust multiplexed format. Application in CRC cellular scenarios, using only nanograms of total RNA, allowed differential profiling of basal and pathway-activated states across five cell lines, revealing distinct RNA methylation patterns associated with diverse biological and genetic backgrounds. Moreover, as proof of concept, an octuple-detection configuration was implemented for parallel analysis of the four methylations in two cell types, wild-type and harboring the clinically relevant KRAS G12V mutation, allowing the evaluation of associations between the target epimark expression levels and this oncogenic point mutation.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130180"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric swab and turbidimetric sensing for dual-mode point-of-use testing of demeton pesticide in foods based on oxidase-like Cu₂O@Ag nanoprobe.","authors":"Yi Liu, Yinxia Yang, Jialin Liang, Lei Han, Qian Zhao, Jie Luo, Shi Gang Liu","doi":"10.1016/j.talanta.2026.130192","DOIUrl":"10.1016/j.talanta.2026.130192","url":null,"abstract":"<p><p>Dual-mode sensing can enhance detection reliability by cross-validating results from distinct signals. Herein, a colorimetric and turbidimetric dual-mode sensing platform based on Cu₂O@Ag nanozyme with robust oxidase-like activity is developed for point-of-use (POU) detection of demeton. The Cu₂O@Ag efficiently catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), producing concurrent absorbance and turbidity signals. Demeton blocks active sites and suppresses catalytic activity in a concentration-dependent manner. Based on the principle, two modes for POU testing of demeton are developed. The first mode is based on a smartphone-assisted colorimetric swab and shows a linear range of 50 - 4000 ng/mL with the detection limit of 0.190 ng/mL. The other is based on a portable turbidimeter and exhibits a linear detection range of 50 to 3000 ng/mL with a detection limit of 2.997 ng/mL. The approaches are successfully validated in real food samples including baby cabbage, lettuce, cilantro, and tea leaves. This work presents a rapid, low-cost, and instrument-flexible dual-mode sensing strategy for on-site analysis of demeton residue. Also, the dual-mode detection based on colorimetric and turbidimetric signals for the first time offers a novel insight for dual-mode sensing.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130192"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing spectral interpretability and band selection prior to prediction model development via CAKE.","authors":"Minh-Quan Nguyen, Mizuki Tsuta, Mito Kokawa","doi":"10.1016/j.talanta.2026.130168","DOIUrl":"10.1016/j.talanta.2026.130168","url":null,"abstract":"<p><p>A direct, singular causal relationship between the objective variable and spectral data underpins reliable and robust prediction models that avoid spurious correlations. However, machine learning models often lack causal interpretability due to their \"black-box\" nature. To address this, we developed the Causal Analysis via Kernel Estimation (CAKE), a framework that reveals single-component bands using only variables from the calibration model. CAKE employs an information-theoretic approach by calculating the differences in mutual information between variables and regression residuals to indicate causal direction. A Kernel Density Estimation (KDE) classifier further distinguishes causal structures prone to spurious correlations. The framework was optimized on simulated data and validated on real-world spectral measurements. Seven causal structures were constructed for the simulated data, while real-world data came from near-infrared and fluorescence spectroscopy of three-solvent mixtures (dimethyl sulfoxide, ethylene glycol, and glycerol). Three causality types - spectral bands with a single direct cause, hidden causes, and confounders - were distinctly characterized by density functions within the framework, enabling the accurate classification of all single-component bands into their respective causal structures. Compared with post-hoc approaches, such as external model validation and common variable selection indices, CAKE identifies reliable spectral bands that exclude spurious correlations across diverse spectroscopic applications while operating independently of the calibration process and requiring no prior knowledge of pure spectra. Although CAKE is designed as a pre-calibration method rather than a prediction-optimized variable selector, it achieves improved predictive accuracy, demonstrating that causal reliability and practical performance are not mutually exclusive.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130168"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicomponent recognition and on-site detection of oral antidiabetic drugs based on a host-guest fluorescent sensor array.","authors":"Hong-Yuan Hu, Qing Tang, Qing Fan, Jin-Xian Luo, Nan Dong, Zhu Tao, Ying Huang","doi":"10.1016/j.talanta.2026.130165","DOIUrl":"10.1016/j.talanta.2026.130165","url":null,"abstract":"<p><p>Hypoglycemia, a serious complication of diabetes, requires prompt identification of its underlying cause. Unlike conventional lock-and-key methods, which generally require a dedicated sensor for each analyte, we constructed a supramolecular fluorescent sensor array for multiplex oral antidiabetic drug (OAD) detection using only two sensing units: cucurbit[8]uril (Q[8]) as the host, along with a synthesized bpep-Am derivative and palmatine as fluorescent probes. Upon introduction of OADs, competitive guest displacement generates differential fluorescence \"fingerprints\". In combination with linear discriminant analysis, the array fully discriminated five OADs (metformin, phenformin, buformin, rosiglitazone, and sitagliptin), with a detection limit as low as 0.218 μM, 100% classification accuracy in artificial urine and guinea pig serum, and >93% recovery for spiked sitagliptin and phenformin. The sensor array was further integrated into a smartphone-enabled portable device for rapid on-site visual quantification of sitagliptin. This strategy provides a promising tool for OAD screening and assessment of hypoglycemia etiology.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130165"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A light-activated one-pot ERA/CRISPR-Cas12a assay for cost-effective dual-mode detection of HBV DNA.","authors":"Xuan Zhou, Lang Wang, XiaoZhen Peng, Mengzhao Liu, Yue Li, Fang Gui, Lanying Zhou, Zhongliang Deng","doi":"10.1016/j.talanta.2026.130329","DOIUrl":"10.1016/j.talanta.2026.130329","url":null,"abstract":"<p><p>Light-activatable CRISPR-Cas systems offer an effective strategy to overcome the kinetic incompatibility in one-pot nucleic acid assays; however, their practical application remains limited by high reagent cost, the lack of compatible reaction buffers, and reliance on instrument-dependent readout. In this work, we developed a cost-effective light-controlled one-pot ERA/CRISPR-Cas12a platform for rapid hepatitis B virus (HBV) DNA detection. NPOM-caged crRNA was employed to temporarily suppress Cas12a activity during amplification and to trigger target-dependent trans-cleavage by a brief 30 s UV irradiation. By replacing RPA with ERA, the per-reaction reagent cost of the one-pot assay was reduced from approximately US$6.0 to US$2.0, corresponding to a reduction of approximately 66.7%, while maintaining comparable analytical performance. In addition, a PEG-free unified buffer was established through systematic optimization, enabling efficient integration of ERA and Cas12a reactions in a sealed single-tube format. The proposed platform provided dual-mode readout through fluorescence and lateral flow assay (LFA), with a limit of detection of 1 copy μL^-1 and a total assay time of 30 min. Clinical evaluation using 79 serum samples showed a sensitivity of 100% for fluorescence readout and 96.3% for LFA, with excellent agreement with qPCR (Cohen's κ = 0.94 - 1.00). Owing to its low cost, high sensitivity, and operational simplicity, this platform represents a promising tool for rapid HBV molecular diagnosis in both centralized laboratories and resource-limited settings.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130329"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148676600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-substrate-based full-color fluorescent probe TPE-4PA@Tb/Eu-GMP-acac ICPs for alkaline phosphatase detection and its on-site application for algal bloom alert.","authors":"Yuanyue Wu, Chunqian Yu, Yiwen Min, Changshun Chen, Ke Zhou, Wanying Zhai, Tianshu Zhou, Jingjing Deng","doi":"10.1016/j.talanta.2026.130159","DOIUrl":"10.1016/j.talanta.2026.130159","url":null,"abstract":"<p><p>The development of rapid, on-site analytical methods and test strips for alkaline phosphatase activity (APA) is crucial for addressing drinking water safety crises, as it enables eutrophication assessment and algal bloom warnings under phosphorus-limited conditions. In this study, we incorporate aggregation-induced emission luminogen (AIEgen) guest TPE-4PA into bimetallic lanthanide-based infinite coordination polymers (Tb/Eu-GMP ICPs). TPE-4PA exhibits unprecedented binding preference for Tb³⁺ over Eu³⁺, triggering coordination-induced emission (CIE) at 450 nm and modulating the antenna effect (AE) and the tandem energy transfer (TER) from Tb³⁺ to Eu³⁺. Subsequently, acetylacetone (acac) was further introduced to yield TPE-4PA@Tb/Eu-GMP-acac ICPs with an appropriate full-color fluoresce response capability. The addition of alkaline phosphatase (ALP) hydrolyzes GMP and TPE-4PA dual substrates, resulting in reduced characteristic emission of the bimetallic Ln-ICP host at 493, 548, 593, 619 and 703 nm, increased monomer emission of TPE-4PA at 392 nm, as well as the solvent-driven AIE of the enzymatic product TPE-4OH at 430 nm, which constitutes a novel ratiometric ALP sensing mechanism with pale-purple to blue fluorescence color shifts. Featuring high sensitivity, doubly assured selectivity, and operational simplicity, this \"all-in-one\" probe enables real-time APA monitoring via dual-substrate kinetics and is suitable for on-site ALP test strip development. Moreover, the unique color transition point of the dual-substrate-based TPE-4PA@Tb/Eu-GMP-acac ICPs probe can be designed to signal an APA surge prior to algal bloom onset, which holds significant potential for the development of microalgae pollution early-warning systems, thereby safeguarding drinking water safety.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130159"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive, self-calibrating cortisol immunosensor enabled by dual-modal CuMOF-PEI@AuNPs@HRP immunoreceptor.","authors":"Xinyi Wang, Minghao Wang, Wenbin Zhang, Dewang Yan, Liubing Kong, Gaofeng Wang","doi":"10.1016/j.talanta.2026.130164","DOIUrl":"10.1016/j.talanta.2026.130164","url":null,"abstract":"<p><p>Accurate cortisol monitoring is essential for stress assessment and health management, yet reliable quantification remains challenging because signal fluctuations and matrix effects can compromise the performance of conventional single-mode immunosensors. Herein, we developed a fluorescence-colorimetric (FL-CL) dual-modal immunosensor based on a CuMOF-PEI@AuNPs@HRP (CPA) immunoreceptor for cortisol determination. The integrated sensing architecture generates correlated fluorescence and colorimetric responses from the same recognition event, which are combined through a normalized dual-signal output to improve calibration reliability. Under optimized conditions, the proposed sensor exhibited a detection limit of 0.22 ng/mL and a linear range of 3.1-200 ng/mL, covering physiologically relevant cortisol concentrations in saliva. Compared with single-modal assays and commercial kits, the proposed platform demonstrates enhanced sensitivity, improved correlation, good stability, and batch reproducibility. By integrating signal amplification, dual-modal readout, and simplified immunoreceptor fabrication within a single sensing platform, this work provides a reliable strategy for cortisol monitoring and offers potential for extension to other biomarker detection systems.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130164"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-integrated bifunctional sensing platform for visual detection of ampicillin and tetracycline in animal manure.","authors":"Ming-Ming Chen, Lu-Chen Cheng, Xiao-Yu Dong, Fang-Jie Zhao, Peng Wang","doi":"10.1016/j.talanta.2026.130173","DOIUrl":"10.1016/j.talanta.2026.130173","url":null,"abstract":"<p><p>Antibiotic residues in environmental matrices pose escalating threats to ecosystems and human health, creating demand for rapid detection. Here, we present a portable smartphone-integrated bifunctional sensor based on Fe-CDs that enables sequential colorimetric and fluorescent detection of ampicillin (Amp) and tetracycline (TC). The Fe-CDs, synthesized via a one-pot hydrothermal process, exhibit stable fluorescence and intrinsic peroxidase-mimicking activity. Ampicillin inhibits the Fe-CDs nanozyme-catalyzed TMB/H₂O₂ chromogenic reaction via •OH consumption, allowing visual colorimetric quantification. Meanwhile, tetracycline strongly quenches the fluorescent signal of Fe-CDs through an inner filter effect, enabling a sensitive fluorescence readout. This bifunctional assay achieves high sensitivity (LOD as low as ∼0.012 μg/mL for Amp and 0.005 μg/mL for TC). By incorporating Fe-CDs onto paper test strips and analyzing with smartphone cameras, the platform allows real-time, rapid quantification of antibiotics in complex matrices. In manure samples, the sensor's results (recoveries ∼94.4-104.8%) agree well with LC-MS/MS measurements, underscoring its accuracy and robustness. Overall, the portable bifunctional Fe-CDs sensor provides a sensitive, field-deployable tool for environmental antibiotic surveillance.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130173"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to 'Design and development of lab-on-a-centrifuge based on liquid-core waveguide enhancement technology for colorimetric detection of soil Cr(VI) from sample-to-answer' [Talanta 305 (2026), 129673].","authors":"Bingyu Mu, Xing Na, Zhengqin Shi, Zhe Liu, Jixin Liu","doi":"10.1016/j.talanta.2026.130177","DOIUrl":"10.1016/j.talanta.2026.130177","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130177"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Spatial confinement-enhanced CHA system based on tetrahedral DNA nanostructures for sensitive in situ mRNA analysis\" [Talanta 306 (2026) 129764].","authors":"Yujie Wang, Tongtong Pan, Mengru Li, Xiaoyan Dai, Siyong Zhang, Hongmei Fu, Ying Chen, Chang Xue, Zhifa Shen, Liang Xu","doi":"10.1016/j.talanta.2026.130096","DOIUrl":"10.1016/j.talanta.2026.130096","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130096"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148215804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}