Talanta最新文献

{"title":"Functional DNA-Zn²⁺ coordination nanospheres for sensitive imaging of 8-oxyguanine DNA glycosylase activity in living cells.","authors":"Yayun Yang, Nan Zhang, Wei Jiang","doi":"10.1016/j.talanta.2024.126779","DOIUrl":"10.1016/j.talanta.2024.126779","url":null,"abstract":"<p><p>Sensitive monitoring of human 8-oxyguanine DNA glycosylase (hOGG1) activity in living cells is helpful to understand its function in damage repair and evaluate its role in disease diagnosis. Herein, a functional DNA-Zn²⁺ coordination nanospheres was proposed for sensitive imaging of hOGG1 in living cells. The nanospheres were constructed through the coordination-driven self-assembly of the entropy driven reaction (EDR) -deoxyribozyme (DNAzyme) system with Zn²⁺, where DNAzyme was designed to split structure and assembled into the EDR system. When the nanospheres entered the cell, the competitive coordination between phosphate in the cell and Zn²⁺ leaded to the disintegration of the nanospheres, releasing DNA and some Zn²⁺. The released Zn²⁺ acted as a cofactor of DNAzyme. In the presence of hOGG1, the EDR was completed, accompanied by fluorescence recovery and the generation of a complete DNAzyme. With the assistance of Zn²⁺, DNAzyme continuously cleaved substrates to produce plenty of fluorescence signals, thus achieving sensitive imaging of hOGG1 activity. The nanospheres successfully achieved sensitive imaging of hOGG1 in human cervical cancer cells (HeLa), human non-small cell lung cancer cells and human normal colonic epithelial cells, and assayed changes in hOGG1 activity in HeLa cells. This nanospheres may provide a new tool for intracellular hOGG1 imaging and related biomedical studies.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent/colorimetric probe for the detection of Cr(Ⅵ) based on MIL-101(Fe)-NH₂ with peroxidase-like activity.","authors":"Shengyuan Zhang, Haiyan Li, Dezhi Yang, Yaling Yang","doi":"10.1016/j.talanta.2024.126785","DOIUrl":"10.1016/j.talanta.2024.126785","url":null,"abstract":"<p><p>In the present research, Fe-based metal-organic frameworks (MIL-101(Fe)-NH₂) nanoparticles were synthesized by simple solvothermal methods and used to assay Cr(Ⅵ). The MIL-101(Fe)-NH₂ performs dual functions: the 2-aminoterephthalic acid (NH₂-BDC) ligand endows a strong fluorescence emission, and the Fe metal nodes are able to facilitate the oxidation of 3,3',5,5'- tetramethylbenzidine (TMB) directly, resulting in the generation of oxidized-TMB (ox-TMB). Our research results showed that reducing agents such as ascorbic acid (AA) can collapse the structures of MIL-101(Fe)-NH₂ because of the reduction of Fe³⁺ by AA, resulting in release of NH₂-BDC. In the presence of Cr(Ⅵ), the fluorescence intensity of the MIL-101(Fe)-NH₂ + AA system will be decreased due to the competitive reduction of Fe³⁺ and Cr(Ⅵ). Nevertheless, Cr(Ⅵ) can significantly accelerate the oxidation of TMB by MIL-101(Fe)-NH₂ as it boosts the electron transfer rate between Fe³⁺ and Fe²⁺. Therefore, a fluorescent/colorimetric dual-mode platform was developed for the detection of Cr(Ⅵ) with an extensive linear range (7.5-750 μg/L and 13.3-1000 μg/L) as well as a remarkably low detection limit (0.99 μg/L and 1.98 μg/L). This MOF with the ability to release ligands not only provides inspiration for the design of new luminescent materials, but also offers a novel and reliable solution for the detection of Cr(Ⅵ).</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescence hyperspectral imaging technology combined with chemometrics for kiwifruit quality attribute assessment and non-destructive judgment of maturity.","authors":"Zhiyong Zou, Qianlong Wang, Qingsong Wu, Menghua Li, Jiangbo Zhen, Dongyu Yuan, Yuchen Xiao, Chong Xu, Shutao Yin, Man Zhou, Lijia Xu","doi":"10.1016/j.talanta.2024.126793","DOIUrl":"10.1016/j.talanta.2024.126793","url":null,"abstract":"<p><p>Dry matter content (DMC), firmness and soluble solid content (SSC) are important indicators for assessing the quality attributes and determining the maturity of kiwifruit. However, traditional measurement methods are time-consuming, labor-intensive, and destructive to the kiwifruit, leading to resource wastage. In order to solve this problem, this study has tracked the flowering, fruiting, maturing and collecting processes of Ya'an red-heart kiwifruit, and has proposed a non-destructive method for kiwifruit quality attribute assessment and maturity identification that combines fluorescence hyperspectral imaging (FHSI) technology and chemometrics. Specifically, first of all, three different spectral data preprocessing methods were adopted, and PLSR was used to evaluate the quality attributes (DMC, firmness, and SSC) of kiwifruit. Next, the differences in accuracy of different models in discriminating kiwifruit maturity were compared, and an ensemble learning model based on LightGBM and GBDT models was constructed. The results indicate that the ensemble learning model outperforms single machine learning models. In addition, the application effects of the 'Convolutional Neural Network'-'Multilayer Perceptron' (CNN-MLP) model under different optimization algorithms were compared. To improve the robustness of the model, an improved whale optimization algorithm (IWOA) was introduced by modifying the acceleration factor. Overall, the IWOA-CNN-MLP model performs the best in discriminating the maturity of kiwifruit, with Accuracy_test of 0.916 and Loss of 0.23. In addition, compared with the basic model, the accuracy of the integrated learning model SG-MSC-SEL was improved by about 12%-20 %. The research findings will provide new perspectives for the evaluation of kiwifruit quality and maturity discrimination using FHSI and chemometric methods, thereby promoting further research and applications in this field.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142118671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive SERS detection of melamine based on 3D Ag@porous silicon photonic crystal.","authors":"Wei Li, Shijie Dai, Xiang Li, Qianjin Li, Jianlin Li","doi":"10.1016/j.talanta.2024.126789","DOIUrl":"10.1016/j.talanta.2024.126789","url":null,"abstract":"<p><p>The stability, reproducibility and engineering of SERS substrate faces a great challenge in melamine SERS assay. In this work, a simple, highly sensitive, stable and cost-efficient SERS detection platform for melamine was established based on its Raman fingerprints spectrum. The Ag@ porous silicon photonic crystal (Ag@PPC) was prepared as the 3D SERS substrate by electrochemical etching and magnetron sputter technology. The main influence factors for the preparation of SERS substrate were investigated in detail. The analytical enhancement factor of the 3D SERS substrate can reach to 2.6 × 10⁸. The 3D SERS detection platform showed a wide linear detection range of 10^-4∼10 mg L^-1 and a low limit of detection of 0.1 μg L^-1 for melamine. Moreover, such detection platform showed good stability, high reproducibility and high recovery rates for melamine. The 3D Ag@PPC SERS substrate can be easily prepared and engineered, displaying a great potential application in food safety field.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing green zinc oxide nanoparticles as a sensing platform for ascorbic acid.","authors":"Andressa N R Leal, Winnie Q Brandão, Maurício F de Aguiar, Dionísio G Kór, Emanoel L T França, Celso P de Melo, Yeda M B de Almeida","doi":"10.1016/j.talanta.2024.126769","DOIUrl":"10.1016/j.talanta.2024.126769","url":null,"abstract":"<p><p>We prepared zinc oxide nanoparticles (ZnO NPs) via a green synthesis and used them for the fluorescence sensing of ascorbic acid (AA). For obtaining these nanoparticles, we used an extract from Batavia lettuce as a reducing agent for zinc acetate in a simple, fast, and environmentally friendly synthesis. The ZnO NPs were characterized by X-ray diffractometry (XRD), ultraviolet-visible spectroscopy (UV-vis), Fourier Transform Infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), photoluminescence, point of zero-charge (pH_pzc), and chromaticity studies. We verified that the ZnO NPs had an average diameter of 6 nm, with a wurtzite crystalline structure, and when excited at 320 nm emitted radiation in the blue region. The methodology for AA detection is based on the observed increase in fluorescence of the molecule complex formed on the ZnO NPs surface after 20 min of interaction. The results indicated that the proposed technique of analysis is fast, simple, and highly sensitive, with a detection limit for AA of 5.15 μM. Furthermore, the nanoparticles presented excellent photostability for at least 30 days, and low sensitivity to other biological organic molecules. The green ZnO NPs also exhibited an efficient response to the presence of AA in actual complex samples, suggesting that the platform here proposed can find use in clinical analysis protocols.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of monoclonal antibodies recognizing pharmacologically active metabolites of metamizole based on rational hapten design and their application in the detection of animal-derived food.","authors":"Linwei Zhang, Jiacan Wang, Yiting Wang, Hao Wen, Mingyue Ding, Jiaxu Xiao, Hongfei Yang, Xiaoming Pan, Shiyun Han, Dapeng Peng","doi":"10.1016/j.talanta.2024.126753","DOIUrl":"10.1016/j.talanta.2024.126753","url":null,"abstract":"<p><p>Metamizole (MET) is an antipyretic and analgesic drug, the illegal use of which can result in residues of MET metabolites in edible tissues of animals. In this study, a computational chemistry-assisted hapten screening strategy was used to screen for the optimal immunogenic hapten-A and the optimal coating antigen hapten-G-OVA. A monoclonal antibody capable of recognizing two pharmacologically active metabolites of MET, 4-methylamidinoantipyrine (MAA) and 4-aminoantipyrine (AA), was prepared from the hapten-A. The antibody showed excellent specificity for MAA and AA and almost no cross-reactivity with the pharmacologically inactive metabolites 4-formamidinoantipyrine (FAA) and 4-acetamidinoantipyrine (AAA). An ic-ELISA was developed for the simultaneous detection of MAA and AA in animal-derived food, the limits of detection for MAA ranged from 0.93 to 1.18 μg/kg, while those for AA ranged from 1.74 to 4.61 μg/kg. The recovery rate fell within the range of 82 %-110 %, with a coefficient of variation less than 16.39 %.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cascade internal electric field dominated carbon nitride decorated with gold nanoparticles as SERS substrate for thiram assay.","authors":"Shuting Zhang, Jingxuan Pei, Yanfang Zhao, Xiang Yu, Lei Yang","doi":"10.1016/j.talanta.2024.126762","DOIUrl":"10.1016/j.talanta.2024.126762","url":null,"abstract":"<p><p>The development of valid chemical enhancement strategy with charge transfer (CT) for semiconductors has great scientific significance in surface-enhanced Raman scattering (SERS) technology. Herein, a phosphorus doped crystalline/amorphous polymeric carbon nitride (PCPCN) is fabricated by a facile molten salt method, and is employed as a SERS substrate for the first time. Upon the synergies of phosphatization and molten salt etching, PCPCN owns a cascaded internal electric field (IEF) due to the formation of p-n homojunction (interface-IEF) and crystalline/amorphous homojunction (bulk-IEF). The interface-IEF and bulk-IEF could effectively suppress the recombination of charge carriers and promote electron transfer between PCPCN and target methylene blue (MB), respectively. The strong CT interaction endows PCPCN substrate with superior SERS activity with an enhancement factor (EF) of 5.53 × 10⁵. Au nanoparticles (Au NPs) are subsequently decorated on PCPCN to introduce electromagnetic enhancement for a better SERS response. The Au/PCPCN substrate allows to reliably detect trace crystal violet, as well as the thiram residue on cherry tomato. This work offers an integrated solution to enhance CT efficiency based on collaborative homojunction and internal electric field, and may inspire the design of novel semiconductor-based SERS substrates.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of shape-controlled covalent organic frameworks for light scattering detection of iron and chromium ions.","authors":"Zheng-Fen Pu, Qiu-Lin Wen, Bi-Chao Wu, Chun-Hua Li, Rong Sheng Li, Jian Ling, Qiue Cao","doi":"10.1016/j.talanta.2024.126682","DOIUrl":"10.1016/j.talanta.2024.126682","url":null,"abstract":"<p><p>Fabricating covalent organic frameworks with different morphologies based on the same structural motifs is both interesting and challenging. Here, a TTA-TFP-COF was synthesized by both solvothermal and room temperature methods, with 2,4,6-Tris(4-aminophenyl)-1,3,5-triazine (TTA) and 1,3,5-tris(4-formylphenyl)-benzene (TFP) as raw material. Using different synthesis conditions and adding aniline and benzaldehyde as regulators in the synthesis process, we found that these processes could slow down the reaction speed, increase the exchange and metathesis reactions of dynamic reversible reactions, and improve the reversibility of the reaction system. Thus, controllable synthesis of TTA-TFP-COF with different morphologies, including micro-particles, hollow tubes with controllable diameters, and micro-flowers was achieved. Our further study found that metal ions, Fe³⁺ and Cr³⁺ ions, could coordinate with N and O in TTA-TFP-COF and partially destroy the structure of TTA-TFP-COF. The particle size of the TTA-TFP-COF became smaller, thus resulting in the decrease of the light scattering intensity of the COF. An excellent linear relationship exists between the light scattering changes (ΔI) and metal ions concentration (c) from 2.0 to 350.0 μM for Fe³⁺ and 40.0-800.0 μM for Cr³⁺, respectively. Thus, rapid and selective analytical methods for detecting metal ions were developed by TTA-TFP-COF here.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesoporous DNA-Co@C nanofibers knitted aptasensors performing onsite determination of trace kanamycin residues.","authors":"Xueli Yuan, Jing Kong, Ying Xie, Xinxin Liu, Wei Zhang, Tao Liu, Zhenyu Chu, Wanqin Jin","doi":"10.1016/j.talanta.2024.126676","DOIUrl":"10.1016/j.talanta.2024.126676","url":null,"abstract":"<p><p>The abuse of kanamycin (KAN) poses an increasing threat to human health by contaminating agricultural and animal husbandry products, drinking water, and more. Therefore, the sensitive detection of trace KAN residues in real samples is crucial for monitoring agricultural pollution, ensuring food safety, and diagnosing diseases. However, traditional assay techniques for KAN rely on bulky instruments and complicated operations with unsatisfactory detection limits. Herein, we developed a novel label-free aptasensor to achieve ultrasensitive detection of KAN by constructing mesoporous DNA-cobalt@carbon nanofibers (DNA-Co@C-NFs) as the recognizer. Leveraging the extended π-conjugation structure, prominent surface area, and abundant pores, the Co@C-NFs can effectively load aptamer strands via π-π stacking interactions, serving as KAN capturer and reporter. Due to the change in DNA configuration upon binding KAN, this aptasensor presented an ultralow detection limit and ultra-wide linear range, along with favorable precision and selectivity. Using real tap water, milk, and human serum samples, the aptasensor accurately reported trace KAN levels. As a result, this convenient and rapid autosensing technique holds promise for onsite testing of other antibiotic residues in agriculture, food safety, and clinical diagnosis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A gold nanoparticle-enhanced dCas9-mediated fluorescence resonance energy transfer for nucleic acid detection.","authors":"Yao Yang, Shanshan Zhai, Li Zhang, Yuhua Wu, Jun Li, Yunjing Li, Xiaofei Li, Longjiao Zhu, Wentao Xu, Gang Wu, Hongfei Gao","doi":"10.1016/j.talanta.2024.126978","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126978","url":null,"abstract":"<p><p>Clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas proteins coupled with pre-amplification have shown great potential in molecular diagnoses. However, the current CRISPR-based methods require additional reporters and time-consuming process. Herein, a gold nanoparticle (AuNP)-enhanced CRISPR/dCas9-mediated fluorescence resonance energy transfer (FRET) termed Au-CFRET platform was proposed for rapid, sensitive, and specific detection of nucleic acid for the first time. In the Au-CFRET sensing platform, AuNP was functionalized with dCas9 and used as nanoprobe. Target DNA was amplified with FAM-labeled primers and then precisely bound with AuNP-dCas9. The formed complex rendered the distance between AuNP acceptor and FAM donor to be short enough for the occurrence of FRET, thus resulting in fluorescence quenching. Moreover, AuNPs were demonstrated to enhance binding efficiency of dCas9 to target DNA in Au-CFRET system. The key factors regarding the FRET efficiency were analyzed and characterized in detail, including the length of donor/acceptor and the size of AuNPs. Under the optimal conditions, Au-CFRET could determinate CaMV35S promoter of genetically modified rice as low as 21 copies μL^-1. Moreover, Au-CFRET sensing system coupled with one-step extraction and recombinase polymerase amplification can identify the genuine plant seeds within 30 min from sampling to results at room/body temperature without expensive equipment or technical expertise, and requires no additional exogenous reporters. Therefore, the proposed sensing platform significantly simplified the system and shortened the assay time for nucleic acid diagnoses.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}