Talanta最新文献

{"title":"Lab-created conductive filament based on nickel and graphite particles: An attractive material for the additive manufacture of enhanced electrochemical sensors for non-enzymatic and selective glucose sensing.","authors":"Natalia M Caldas, Lucas V de Faria, Amanda G Batista, Anderson O Alves, Cassiano C de Souza, Pedro H S Borges, Edson Nossol, Renato C Matos, Diego P Rocha, Felipe S Semaan, Rafael M Dornellas","doi":"10.1016/j.talanta.2025.127686","DOIUrl":"10.1016/j.talanta.2025.127686","url":null,"abstract":"<p><p>Developing tailor-made conductive filaments has emerged as a promising niche for producing affordable and high-performance 3D-printed electrochemical sensors. In this context, we propose a novel conductive filament based on graphite, nickel, and polylactic acid (G/Ni/PLA) for the fabrication of non-enzymatic electrochemical sensors aimed at glucose (GLU) determination, a key biomarker in diabetes diagnosis. The materials were thoroughly characterized using morphological, structural, elemental, and electrochemical techniques, which confirmed the effective incorporation of G and Ni into the thermoplastic matrix. Special emphasis was placed on the electrochemical conversion of Ni²⁺ in an alkaline medium (0.1 mol L⁻¹ NaOH) into redox-active species (Ni(OH)₂ and NiOOH), which mediate the electrocatalytic oxidation of GLU. Additionally, the influence of varying nickel contents (7.5 %, 10 %, and 12.5 % wt.) on the electrochemical response towards GLU was systematically investigated, with the best performance observed at the highest nickel loading. The innovative 3D-printed G/Ni/PLA sensor was integrated with a batch injection analysis (BIA) system for rapid and sensitive amperometric detection of GLU in artificial biological fluids. The sensor demonstrated a wide linear range (50-1500 μmol L⁻¹), a low detection limit (2.6 μmol L⁻¹), excellent repeatability (RSD < 9.0 %), and high selectivity, even in the presence of potential interferents such as urea, uric acid, and ascorbic acid. Furthermore, the method was successfully applied to analyze synthetic saliva (a non-invasive sample matrix) and blood plasma under normal and abnormal GLU levels, achieving satisfactory recovery rates ranging from 93 % to 100 %. Therefore, the proposed analytical approach is simple, selective, precise, and accurate, making it highly suitable for non-enzymatic GLU sensing in clinical samples, contributing to the effective diagnosis of diabetes.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127686"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of cross-reactive DNA aptamers to construct the fluorescent sensing array for identifying the origin of Toad Venom.","authors":"Jiwei Li, Guocai Liu, Haixiang Yu, Hongyue Ma, Xiufeng Liu, Jiangwei Tian, Boyang Yu","doi":"10.1016/j.talanta.2025.127705","DOIUrl":"10.1016/j.talanta.2025.127705","url":null,"abstract":"<p><p>Toad venom, a traditional Chinese medicine, has long been used to treat various challenging ailments. Its effectiveness and toxicity can vary depending on the types and concentrations of bufadienolides, which vary from region to region. However, identifying the origin of toad venom is challenging due to the absence of distinct visual characteristics of the original animals. Therefore, developing a scientific and practical method for origin identification is crucial to ensure the safety and efficacy of toad venom. Integrating a fluorescent sensing array with cross-reactive aptamers provides a promising solution to this issue. We isolated cross-reactive aptamers using a combination of complex target-directed SELEX and convergent selection strategies. During the selection process, we used an immobilized stem-loop library to select aptamers and evaluated the enrichment rate and pool affinity using gel elution assays. After high-throughput sequencing, we selected three cross-reactive aptamers designated N4.8, N2.4, and S1 that exhibit distinct binding profiles for bufadienolides as the biorecognition elements for the fluorescent sensing array. This sensor is capable of distinguishing toad venom from different origins with high accuracy (98.7 %), offering convenient operation, and providing a new method for detecting the origin of toad venom.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127705"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MXene-mediated fluorescent recombinase polymerase amplification for rapid and sensitive DNA methylation biosensing.","authors":"Bingshan Guo, Jiaoying Peng, Yongzhi Wang, Ying Chen, Ran Li, Xuemei Xu, Yongchao Yao, Wenchuang Walter Hu","doi":"10.1016/j.talanta.2025.127685","DOIUrl":"10.1016/j.talanta.2025.127685","url":null,"abstract":"<p><p>DNA methylation serves as a promising biomarker for early cancer detection, with its assessment providing crucial insights for prognosis and diagnosis. Herein, we present a novel MXene-mediated fluorescent recombinase polymerase amplification (RPA) strategy that achieves rapid and sensitive non-small cell lung cancer (NSCLC)-associated methylated DNA detection. Remarkably, MXene can effectively quench the fluorescence of fluorescein amidate (FAM) labeled primer, with fluorescence recovery occurring as primers extend to double-stranded DNA and are released during the RPA process. This method exhibits exceptional sensitivity and selectivity, enabling the detection of methylated DNA at concentrations as low as 10 fM, with assay completion achieved in less than 1 h. Its ability to precisely evaluate gene-specific methylation showcases its potential to advance methylation as a disease biomarker, particularly in NSCLC.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127685"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ultrasensitive homogeneous electrochemical strategy for ochratoxin a sensing based on nanoscale PCN-224@MB@Apt.","authors":"Xi Wu, Chenggang Geng, Weiwei Cheng, Zhenjiong Wang, Yan Zhang, Di Wu, Xiaozhi Tang","doi":"10.1016/j.talanta.2025.127695","DOIUrl":"10.1016/j.talanta.2025.127695","url":null,"abstract":"<p><p>In this reasearch, a homogeneous electrochemical sensor based on PCN-224@MB@Apt was fabricated for the ultrasensitive determination of ochratoxin A (OTA). Firstly, nanoscale PCN-224 were synthesized as the nanocarrier to embed the signal probe of methylene blue (MB). Then, the OTA aptamer (Apt) was added and connected to PCN-224@MB via the Zr-O-P bond between Zr metal sites of PCN-224 and phosphate group of Apt as the biogate. When OTA exists, the Apt would preferentially bind with OTA and fall off from PCN-224@MB, leading to the release of MB and generation of differential pulse voltammetry (DPV) response. The DPV response of MB was linearly correlated with the amount of OTA. The optimized PCN-224@MB@Apt sensor showed outstanding detection performance towards OTA with a low detection limit of 2.6 × 10^-5 ng/mL (S/N = 3) and wide linear range (10^-4-10 ng/mL). Meanwhile, the fabricated homogeneous electrochemical sensor exhibited splendid stability, reproducibility, and specificity. To assess the practical applicability, the PCN-224@MB@Apt sensor was applied to detect OTA in real corn samples and desirable recovery rates varying from 83.2 % to 109.6 % were obtained.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127695"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable epidermal sensor patch with biomimetic microfluidic channels for fast and time-sequence monitoring of sweat glucose and lactate.","authors":"Chenchen Liao, Shuyi Li, Chaohuan Yang, Chengyu Du, Hecheng Yao, Zhiwu Han, Urszula Stachewicz, Yan Liu","doi":"10.1016/j.talanta.2025.127683","DOIUrl":"10.1016/j.talanta.2025.127683","url":null,"abstract":"<p><p>Wearable sweat sensors enable non-invasive tracking and monitoring of human physiological information, which is expected to attract wide interest and rapid development in dietary health management and disease prevention. Unfortunately, sweat sensors are limited by rapid evaporation and low secretion rates of sweat. Herein, a sweat detection patch is proposed, which integrates bionic microchannels and multiparameter electrochemical sensors. The microfluidic channel (5°), which mimics ginkgo biloba veins, provided a 40 % higher flow rate compared to the normal channel (0°). Combined with burst pressure, the bionic channel enabled unidirectional transport of 6 μL sweat, effectively avoiding the mixing of old and new sweat. The electrochemical sensors possessed excellent specificity recognition, stability and durability, and have been used to detect substances in sweat, in particular to analyze changes in glucose concentration at different dietary intakes and changes in lactate metabolism after exercise. The rapid collection effect of the microchannels on trace sweat and the fast response of sensors have broad application prospects in real-time human health monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127683"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-field and far-field competitive couplings between plasmonic nanodisk array and nanoparticles for rapid and facile heparin assay.","authors":"Shuwen Chu, Huizhen Yuan, Yuzhang Liang, Cheng Yang, Qiang Liu, Wei Peng","doi":"10.1016/j.talanta.2025.127690","DOIUrl":"10.1016/j.talanta.2025.127690","url":null,"abstract":"<p><p>Plasmonic biosensors hold an enticing prospect for drug testing and disease diagnostics as they have demonstrated impressive superiority in ultrasensitive and label-free detection. In this paper, we demonstrate a plasmonic sensing strategy for the heparin assay by leveraging near-field and far-field competitive couplings between gold (Au) nanoparticles (NPs) and nanodisk (ND) array. Specifically, the near-field coupling of the ND array and Au NPs binding to its surface results in a spectral redshift. Meanwhile, the far-field coupling of dispersed Au NPs in mixed solution and ND array induces a spectral blueshift. As a result, heparin concentration-dependent near-field and far-field competitive couplings determine the direction and amount of the spectral shift. Compared with existing detection technologies, this sensing strategy also presents a balance point, enabling rapid assessment of heparin dosage safety. Additionally, an exceptionally wide dynamic range of 10^-4 μg/mL to 10³ μg/mL, a low detection limit of 29 pg/mL, and excellent selectivity are demonstrated. The analysis of heparin in serum further underscores this assay approach's potential for advancing disease diagnosis and therapeutic monitoring at the point of care.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127690"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of thermal and solvent stable SPME fibers for metabolomics studies performed in living systems.","authors":"Kübra Kahremanoğlu, Karol Jaroch, Paulina Szeliska, Wojciech Filipiak, Bartłomiej Charemski, Karolina Żuchowska, Enes Çetin, Ahmet E Eroğlu, Barbara Bojko, Ezel Boyaci","doi":"10.1016/j.talanta.2025.127646","DOIUrl":"10.1016/j.talanta.2025.127646","url":null,"abstract":"<p><p>Solid phase microextraction (SPME), as a sampling/sample preparation technique, offers unique solutions for the most challenging applications, including metabolomics studies of living systems. However, for global metabolomics it is critical to use an SPME sampler facilitating the extraction of both volatiles and nonvolatiles, which at the same time is compatible with thermal and solvent-assisted desorption. As a promising universal coating, recently hydrophilic-lipophilic balanced (HLB) particles immobilized in PTFE have been introduced as a new SPME sampler to provide a wide-range of analyte coverage and compatibility with solvent and thermal desorption. Thus, making it suitable for both gas and liquid chromatography (GC/LC) based applications. However, its potential in metabolomics has not been investigated to date. In this study, HLB/PTFE SPME fibers were prepared, evaluated with selected polar and non-polar metabolites relevant to biological systems, and validated for cell-line studies. The validation proved that these fibers can extract a wide-range of molecules (LogP: 4.2 to 15.6) with acceptable accuracy (≤19% RE%) and repeatability (intra-day ≤17% and inter-day 12% RSD%). The LOQ was determined to vary between 150.0 and 500.0 ng/mL. Upon validation, the fibers were used in a proof-of-concept study for extraction of endometabolome and exometabolome of melanoma B16F10 and lung cancer LL2 cell lines. The metabolome studies showed that HLB/PTFE fibers provide lower coverage, but for some compounds higher extraction efficiency compared to HLB/PAN fibers used in LC-based metabolomics. Fibers also proved suitable for GC-MS analysis, allowing for the detection of 36 volatile organic compounds in the headspace of the cell lines and RPMI medium.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127646"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FeCuS multilayer nanoflowers loaded with N-CDs dual-mode method for the detection of uric acid and gout risk prediction with the aid of chemometrics.","authors":"Rou Cheng, Chunyan Yan, Zhengyue Xiao, Xiaomin Tang, Peng Xu, Ping Qiu","doi":"10.1016/j.talanta.2025.127700","DOIUrl":"10.1016/j.talanta.2025.127700","url":null,"abstract":"<p><p>Transition metal sulfides have attracted much attention due to their low cost, high stability, adjustable performance, diverse morphology and good catalytic activity. In this work, nitrogen-doped carbon dots (N-CDs) were loaded onto bimetallic sulfides (FeCuS) and FeCuS/N-CDs could act as catalyst and fluorescent probe, which applied to the detection of uric acid (UA) in human blood and urine using dual-model assay (colorimetry and ratiometric fluorescence). The nanozyme has excellent peroxidase-like activity and steady-state kinetic results showed that the material prepared under optimized conditions had better affinity (K_m = 0.42 mM) for the substrate and faster reaction rate (V_max = 14.1 × 10^-8 M s^-1). •OH and O₂^•- were involved in the catalytic process of o-phenylenediamine (OPD) by FeCuS/N-CDs nanozymes. A profound discussion and quantitative analysis about the sensing mechanism of UA and fluorescence quenching mechanism between FeCuS/N-CDs and diaminophenazine (DAP) was performed. According to Parker equation, the inhibition efficiency of DAP in FeCuS/N-CDs was as high as 83 % of the total inhibition efficiency, confirming that the inhibition efficiency mainly came from internal filtering effect (IFE). The detection limits of ratiometric fluorescence method and colorimetric method were as low as 0.13 μM and 0.27 μM, respectively. Finally, principal component analysis was used to successfully distinguish three groups of people (healthy people, potential people and gout patients) and the model has potential value in clinical applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127700"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MALDI-TOF mass spectrometric immunoassay of multiple tumor biomarkers for non-small cell lung cancer screening.","authors":"Mengchen Wang, Chengxiang Zhu, Nan Feng, Yiran Li, Jiahui Sun, Huangxian Ju","doi":"10.1016/j.talanta.2025.127550","DOIUrl":"10.1016/j.talanta.2025.127550","url":null,"abstract":"<p><p>Cancer biomarkers have been facing some issues such as poor accuracy and low sensitivity in the early diagnosis of tumors. Utilizing biotin-labelled peptide as a mass tag (MT), this work proposes a high-throughput biosensing strategy for matrix-assisted laser desorption/ionization-time of flight mass spectrometric (MALDI-TOF-MS) immunoassay of multiple lung cancer biomarkers. Due to little required dosage, satisfied stability, high sensitivity and accuracy, this method can achieve off-site centralized signal detection after on-site sample incubation. The proposed approach has been successfully applied for the detection of carcinoembryonic antigen (CEA), carbohydrate antigen199 (CA199), carbohydrate antigen 125 (CA125) and cytokeratin-19-fragment (CY211) in serum samples from various stages of non-small cell lung cancer. Based on the analysis of multiple parameters and pathological results, significant differences in biomarkers are found in serum samples of lung cancer patients at different stages. More importantly, the analysis of multiple tumor biomarkers can improve the accuracy and sensitivity of early diagnosis. Therefore, the multiple immunoassay based on MALDI-TOF MS exhibits exceptional performance in terms of high throughput, little sample dosage, stability and sensitivity.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127550"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Bright luminescent Zn2GeO4:Mn NP/MXene hydrogel-based ECL biosensor for glioblastoma diagnosis\" [Talanta 276 (2024) 126214].","authors":"Shuanglin Deng, Wenyan Li, Zhenrun Li, Peilin Wang, Qiang Ma","doi":"10.1016/j.talanta.2025.127628","DOIUrl":"10.1016/j.talanta.2025.127628","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"127628"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}