Talanta最新文献

{"title":"Deep learning-assisted Ce/Zr-MOF nanozyme hydrogel sensor for colorimetric/photothermal dual-mode detection of glyphosate.","authors":"Haiting Yu, Ming Xu, Lin Nie, Yukui Tong, Miaomiao Tian","doi":"10.1016/j.talanta.2026.129693","DOIUrl":"10.1016/j.talanta.2026.129693","url":null,"abstract":"<p><p>The extensive application of glyphosate (GLY) in modern agriculture has raised increasing concerns regarding environmental contamination and food safety. In this work, a dual-mode hydrogel sensing platform was constructed based on Ce/Zr-MOF with intrinsic peroxidase-like activity. The synthesized Ce/Zr-MOF efficiently catalyzed the oxidation of TMB into blue oxTMB, enabling colorimetric detection while simultaneously generating a photothermal response. Notably, GLY strongly coordinated with the Ce³⁺/Zr⁴⁺ active centers, effectively inhibiting the catalytic reaction and leading to concentration-dependent attenuation of both color intensity and photothermal heating. Owing to this inhibition mechanism, the hydrogel sensor achieved dual-mode detection of GLY over a wide linear range of 0-500 μM, with low detection limits of 0.13 μM (colorimetric) and 0.08 μM (photothermal). This platform also demonstrates excellent selectivity and long-term stability. Furthermore, a deep learning-based dual-modal concentration prediction network (DMCPNet) was developed to enable rapid and accurate determination of GLY concentrations. The proposed strategy offers reliable on-site detection of GLY and demonstrates the potential of multifunctional hydrogel sensors for environmental and food safety monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"305 ","pages":"129693"},"PeriodicalIF":6.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cysteine exerts surface polarization effect on BiOCOOH for photoelectrochemical detection of small molecules.","authors":"Chunxia Wu, Yaoxuan Jin, Youchao Ding, Xiuming Wu, Lihua Cao, Guang-Li Wang","doi":"10.1016/j.talanta.2026.129687","DOIUrl":"10.1016/j.talanta.2026.129687","url":null,"abstract":"<p><p>The exploration of new kinds of materials with novel sensing mechanisms for photoelectrochemical (PEC) analysis are crucial for advancing efficient PEC detection. This study demonstrated that cysteine (Cys) induced surface polarization effect (SPE) on the surface of the environmentally friendly layered bismuth-based semiconductor material, i.e., BiOCOOH. Based on this finding, we established a detection system for small molecules dehydroisoandrosterone-3-sulfate (DIS) and bisphenol A (BPA) by integrating the SPE sensing mechanism with the aptamer recognition reaction. That was, the Cys bound to the BiOCOOH surface via its thiol group through its interaction with Bi(III) ions, and generated the SPE with a surface polarization electric field, thereby enhancing photocurrent. This detection system demonstrated commendable performance in DIS and BPA analysis, with a linear detection range of 0.1-250 μM for DIS and a detection limit as low as 14 nM. For BPA analysis, the linear range spanned 0.01-100 μM with a detection limit as low as 1.4 nM. This study validates the SPE on extensible semiconductors/polar molecules with potentially extended applications across diverse fields, including multifunctional PEC analysis and the development of highly efficient photocatalysts, photoelectrocatalysts, and solar cells.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"305 ","pages":"129687"},"PeriodicalIF":6.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive chemiluminescent detection of plasma Aβ1-40 based on soluble macromolecular signal amplification carrier.","authors":"Chaolin Guo, Meng Liu, Zhanfei An, Shuaipeng Li, Menglin Cui, Jianqi Nie, Yang Sun, Zhonghu Bai","doi":"10.1016/j.talanta.2026.129688","DOIUrl":"10.1016/j.talanta.2026.129688","url":null,"abstract":"<p><p>The research presents the development of an ultrasensitive chemiluminescent immunoassay (CLIA) for detecting the Alzheimer's disease (AD) biomarker amyloid-β (Aβ) 1-40 in plasma, utilizing novel monoclonal antibodies and an innovative soluble macromolecular signal amplification carrier, Ficoll400-Streptavidin (Ficoll400-SA) conjugates. The study successfully generated two high-affinity monoclonal antibodies (KD ≈ 10^-10 M), designated 2F10 and 12H6, which are epitope-specific and target distinct N-terminal and C-terminal regions of Aβ1-40. These antibodies demonstrated exceptional specificity, exhibiting minimal cross-reactivity (<0.5%) with closely related isoforms like Aβ1-42. The Ficoll400-SA carrier, formed by conjugating multiple streptavidin molecules to the Ficoll400 backbone and further crosslinking, enabled substantial signal amplification by facilitating rapid, homogeneous reactions and high-density enzyme loading capacity. Optimization of assay conditions demonstrated a 13-fold signal enhancement compared to conventional streptavidin-horseradish peroxidase (SA-HRP) systems, achieving an ultra-low detection limit of 0.28 pg mL^-1 and a broad dynamic range (0.54-1000 pg mL^-1). The assay exhibited excellent precision (CV < 8%), robust stability under accelerated and onboard conditions, and outstanding specificity with negligible cross-reactivity. Clinical validation against a commercial Aβ1-40 assay using human plasma samples showed a remarkable correlation (Spearman's ρ = 0.99) and no systematic bias, confirming the assay's accuracy and translational potential. Overall, this integrated approach offers a robust, cost-effective, and ultrasensitive platform for Aβ1-40 quantification, advancing blood-based AD diagnostics and demonstrating the versatility of soluble macromolecular carriers in immunoassay signal amplification.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"305 ","pages":"129688"},"PeriodicalIF":6.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated analysis of ultra-trace ²³⁶U and natural uranium isotopes via integrated sample preparation and ICP-MS/MS detection.","authors":"Jisheng Chen, Youyi Ni, Wenting Bu, Yue Luo, Bingjun Chen, Xuemei Liu, Chuting Yang, Junwen Wu, Hai Wang","doi":"10.1016/j.talanta.2026.129672","DOIUrl":"10.1016/j.talanta.2026.129672","url":null,"abstract":"<p><p>Highly efficient and accurate analysis of ultra-trace anthropogenic ²³⁶U in the presence of natural uranium isotopes is essential for nuclear forensics and environmental monitoring. In this study, we developed an integrated analytical method for the determination of ²³⁴U, ²³⁵U, ²³⁶U, and ²³⁸U by combining highly automated sample preparation with highly sensitive ICP-MS/MS detection. Automated total dissolution was employed to ensure complete extraction of both endogenous and exogenous uranium from solid matrices, while reproducible chemical separation was achieved using an automated platform equipped with regenerable UTEVA resin. This optimized procedure yielded exceptionally low operational blanks for ²³⁶U at femtogram level. Moreover, the final eluent volume was minimized to 1.5 mL, allowing direct introduction into the ICP-MS/MS. By utilizing a membrane desolvation sample introduction system and a novel mass-shift mode (targeting UO₂⁺ species), the ICP-MS/MS sensitivity exceeded 4.1 × 10⁶ cps/ppb, while the ²³⁵UH ⁺ interference formation rate for ²³⁶U was suppressed to 7.1 × 10^-10. Consequently, the achieved detection limits for ²³⁶U and the ²³⁶U/²³⁸U atom ratio were as low as 1.85 fg/g and 5 × 10^-12, respectively. This method was successfully applied to determine the uranium isotopic composition in sediments from the adjacent sea area of the Daya Bay Nuclear Power Plant. These results demonstrate that the proposed method provides a robust and high-throughput solution for accurately quantifying of ultra-trace ²³⁶U in environmental samples, such as those impacted by the global fallout.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"305 ","pages":"129672"},"PeriodicalIF":6.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AptaStick: A 3D-printed aptamer-functionalized stir-stick for selective extraction of bisphenol A from soft drinks.","authors":"Clara Ochoa-Esteso, Alba Roselló-Carrió, Patricia Reboredo-Rodríguez, María Jesús Lerma-García, José Manuel Herrero-Martínez, Enrique Javier Carrasco-Correa","doi":"10.1016/j.talanta.2026.129681","DOIUrl":"10.1016/j.talanta.2026.129681","url":null,"abstract":"<p><p>This work presents AptaStick, a stereolithography 3D-printed stir-stick device functionalized with a bisphenol A (BPA)-specific aptamer for selective extraction from soft drinks. For this purpose, several geometries were evaluated to optimize extraction performance, with a star-shaped design providing the highest extraction efficiency and reproducibility. Under optimized conditions, BPA retention reached 99%, and quantitative elution was achieved within 15 min using acetonitrile/phosphate-buffered saline containing adenosine triphosphate. The method provided a linear response between 2.5 and 2000 μg L^-1 (r² = 0.996), with limits of detection and quantification of 94 and 313 ng L^-1, respectively. Intra- and inter-device precision were 1.1% and 10.7% (relative standard deviation), and the device was reusable for at least four times. Application to commercial soft drinks yielded BPA recoveries from 87.8 to 99.5%. The proposed platform combines additive manufacturing versatility with aptamer selectivity, offering a simple, reusable, and sustainable alternative for selective extraction of BPA, a relevant endocrine-disrupting compound, in beverage analysis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"305 ","pages":"129681"},"PeriodicalIF":6.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From biosensing to herbal discovery: A nanozyme cascade platform for acetylcholinesterase monitoring and inhibitor screening in synthetic and natural sources","authors":"Shuang Cai ,&nbsp;Bao-Wen Fan ,&nbsp;Jia Qin ,&nbsp;Cheng-Yang Pu ,&nbsp;Ya-Jian Qin ,&nbsp;Cong-Ting Wu ,&nbsp;Wen Xu ,&nbsp;Jing Li","doi":"10.1016/j.talanta.2026.129523","DOIUrl":"10.1016/j.talanta.2026.129523","url":null,"abstract":"<div><div>Early diagnosis and therapeutic intervention for Alzheimer's disease (AD) necessitate advanced tools for detecting acetylcholinesterase (AChE) activity and screening AChE inhibitors (AChEIs). This study developed a novel bimetallic MOF nanozyme, Cu–NH₂–88B(Fe), exhibiting significant peroxidase-like activity. This nanozyme was integrated with AChE to construct a dual-enzyme cascade biosensing platform, which achieved highly sensitive AChE detection, with a detection limit of 0.01 mU/mL, and demonstrated excellent accuracy in fetal bovine serum (spiked recoveries: 92.07–111.09%). Additionally, the platform also enabled quantitative assessment of synthetic AChEIs, determining IC₅₀ values for donepezil (9.85 nM), neostigmine (1.41 μM), huperzine A (6.21 μM), and galantamine (611.31 μM), all of which exhibited broad linear ranges and high sensitivity. All four compounds exhibited a broad linear range and excellent sensitivity. Innovatively, the platform was applied to screen AChE inhibitory activity in seven traditional Chinese medicines (TCMs). Crucially, when comparing four extraction methods, ultrasound-assisted extraction (UAE) proved most effective in liberating active compounds. The UAE-obtained extract of <em>Poria cocos</em> (Yunnan) showed the strongest inhibition, achieving an AChE inhibition rate of 39.03% at 0.01 mg/mL. Notably, the screening results across different species, origins, and extraction methods (including the superior UAE) showed high consistency with the classical Ellman method, validating the platform's reliability. This study not only provides a low-cost, easy-to-operate, sensitive, and reliable analytical strategy for AChE activity detection and AChE inhibitor development, but more importantly, it successfully applies nanozyme technology to the complex system of TCMs, offering strong technical support for the screening and preliminary identification of potential anti-AD active components from TCM resources.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129523"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid magnetic solid-phase extraction approach employing a modified graphene oxide nanomaterial for the determination of 20 pharmaceuticals and transformation products in treated urban wastewater","authors":"Ana Belén Martínez-Piernas ,&nbsp;Irene Sánchez-Trujillo ,&nbsp;Carlos Vereda-Alonso ,&nbsp;María del Mar López-Guerrero ,&nbsp;Elisa Isabel Vereda-Alonso","doi":"10.1016/j.talanta.2026.129521","DOIUrl":"10.1016/j.talanta.2026.129521","url":null,"abstract":"<div><div>A magnetic solid-phase extraction (MSPE) method based on a green magnetic graphene oxide material (d-M@GO) was developed and validated for the simultaneous determination of 20 contaminants of emerging concern (CECs), including pharmaceuticals and transformation products, in wastewater using liquid chromatography–high-resolution mass spectrometry (LC-HRMS). The adsorption behaviour of analytes on d-M@GO was investigated through kinetic and equilibrium modelling, indicating mass-transfer-limited adsorption consistent with a linear isotherm. A two-zone kinetic model, accounting for fast and slow adsorption regions, provided the best fitting for most compounds, confirming the coexistence of easily accessible and diffusion-limited adsorption sites.</div><div>The MSPE procedure was optimised to establish the most efficient adsorption and elution conditions. Method validation in treated urban wastewater demonstrated its suitability as a green alternative to conventional solid-phase extraction. Eighteen of the twenty analytes showed recoveries between 70% and 120%, with RSDs below 20%. Low method quantification limits (MQLs) were determined, ranging from 2 to 20 ng/L. Application of the method for the analysis of five wastewater samples enabled the quantification of up to 19 analytes at concentrations ranging from 6.37 to 1321 ng/L. In addition, non-target screening using MS-DIAL open-source software (a platform for untargeted metabolomics and lipidomics data processing) expanded the analytical scope, allowing the tentative identification of 24 additional CECs, five of which were confirmed using reference standards. The combination of target and non-target analyses demonstrates the capability of the method for comprehensive monitoring of CECs in wastewater. With a 60–70% lower carbon footprint than conventional SPE approaches, the d-M@GO-MSPE–LC-HRMS workflow represents a robust, rapid, and sustainable solution.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129521"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunosensors for dual tumor biomarker detection based on ternary electrochemiluminescence using confined CeO2 nanozyme as Co-reactant enhancers for luminol-O2 system","authors":"Chao Lu ,&nbsp;Xue Fan ,&nbsp;Fengna Xi ,&nbsp;Yucheng Zhou","doi":"10.1016/j.talanta.2026.129524","DOIUrl":"10.1016/j.talanta.2026.129524","url":null,"abstract":"<div><div>Sensitive and parallel individual detection of multiple tumor biomarkers is critical for early diagnosis and therapeutic monitoring of pancreatic cancer. Herein, a ternary electrochemiluminescence (ECL) system was developed by employing nanochannel-confined cerium dioxide (CeO₂) nanozyme as co-reactant enhancers for the luminol-dissolved oxygen (DO) system, enabling parallel individual immunoassays of carcinoembryonic antigen (CEA) and carbohydrate antigen 199 (CA199). Vertically-ordered mesoporous silica film (VMSF) was rapidly grown on an indium tin oxide (ITO) electrode, forming nanochannel array with thickness of 97 nm and diameters of 2.7 nm. CeO₂ nanozyme was synthesized <em>in situ</em> within the VMSF nanochannels <em>via</em> electrochemical deposition. Transmission electron microscopy (TEM), high-angle annular dark-field scanning TEM (HAADF-STEM), and energy-dispersive X-ray spectroscopy (EDX) elemental mapping confirmed CeO₂ nanozyme was confined in nanochannels with high dispersion. CeO₂ nanozyme exhibited peroxidase-like catalytic activity and enhanced the ECL intensity of the luminol-DO system by 13.9-fold under near-neutral conditions through catalysis of both oxygen reduction and luminol oxidation. After derivatization of the VMSF outer surface with epoxy groups, antibodies specific to CEA or CA199 were covalently immobilized to construct two immunorecognition interfaces. Target binding formed antigen-antibody complexes, increasing interfacial resistance and hindering diffusion of the ECL emitters, enabling a signal-off immunoassay mode. The resulting immunosensors displayed wide detection linear ranges from 0.1 pg mL⁻¹ to 100 ng mL⁻¹ for CEA and 0.1 × 10⁻³ mU mL⁻¹ to 1.0 × 10⁴ mU mL⁻¹ for CA199, with limit of detection (LOD) of 1.3 fg mL⁻¹ and 0.5 × 10⁻⁴ mU mL⁻¹, respectively. Both immunosensors exhibited good selectivity, reproducibility, and storage stability.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129524"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hollow Sn-doped ZnO nanotubes for synergistically enhanced sensing performance toward 3-hydroxy-2-butanone with preliminary exploration of a multi-parameter intelligent system","authors":"Xiao-Xue Lian,&nbsp;Jing-Wen Liu,&nbsp;Yan Li,&nbsp;Shu-Mei Yang","doi":"10.1016/j.talanta.2026.129512","DOIUrl":"10.1016/j.talanta.2026.129512","url":null,"abstract":"<div><div>Targeted detection of the 3-hydroxy-2-butanone (3H-2B) biomarker represents a feasible and reliable strategy for monitoring <em>Listeria monocytogenes</em>, a highly virulent foodborne pathogen that poses severe threats to public health and food safety. The significance of this study lies in addressing the urgent demand for rapid, sensitive, and on-site detection of <em>Listeria monocytogenes</em>, as traditional detection methods are often time-consuming, labor-intensive, and unsuitable for field applications. Herein, we successfully prepared Sn-doped ZnO hollow nanotubes via an electrospinning process, and further designed a multi-parameter intelligent sensing system based on this material for portable 3H-2B detection. A key improvement of our work is the synergistic integration of Sn doping and morphological engineering, which dramatically enhances gas-sensing performance: Sn doping introduces a high density of oxygen vacancies and free charge carriers, while the tailored hollow nanotube configuration maximizes the accessibility of active sites and facilitates rapid gas diffusion across the sensing interface. The optimized 3% Sn-doped ZnO sensor exhibits excellent 3H-2B sensing performance, with an optimal response of 155.1 to 100 ppm 3H-2B at 200 °C, a rapid response time of 1 s, and a low detection limit of 100 ppb, significantly outperforming undoped ZnO and other Sn-doped counterparts. This sensing system shows promising application prospects in food safety supervision, catering industry, and food processing workshops, enabling rapid on-site monitoring of <em>Listeria monocytogenes</em> contamination. This study not only establishes Sn-doped ZnO hollow nanotubes as a superior sensing platform for 3H-2B but also opens a new avenue for the rapid, sensitive detection of foodborne pathogens, providing technical support for food safety guarantee.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129512"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential adoption of electrochemical biosensors for cancer DNA biomarker detection in liquid biopsies: A systematic review","authors":"Anouk Peymen ,&nbsp;Thijs Van der Snickt ,&nbsp;Alejandro Valverde ,&nbsp;Scott Ailliet ,&nbsp;Karen Zwaenepoel ,&nbsp;Pieter Mestdagh ,&nbsp;Karolien De Wael","doi":"10.1016/j.talanta.2026.129488","DOIUrl":"10.1016/j.talanta.2026.129488","url":null,"abstract":"<div><h3>Background</h3><div>The detection of actionable DNA biomarkers in liquid biopsies is essential for advancing precision oncology. While conventional techniques such as PCR and NGS are highly accurate, their high cost, complexity, and slow turnaround time limit widespread clinical applications. Electrochemical biosensors present a promising alternative through their portability, affordability, and rapid analysis capabilities.</div></div><div><h3>Objective</h3><div>This systematic review evaluates the current status and potential clinical adoption of electrochemical biosensors for detecting cancer DNA biomarkers in liquid biopsies, focusing on their sensitivity and specificity compared to standard PCR-based methods.</div></div><div><h3>Methods</h3><div>A comprehensive search across PubMed, Scopus, and Web of Science was conducted up to August 2025, yielding 1723 articles. After applying PRISMA guidelines with strict inclusion and exclusion criteria, 31 studies were selected. The quality of the selected studies was assessed using the SURE checklist. Data on biosensor design, amplification strategies, detection limits, and clinical applicability were extracted.</div></div><div><h3>Results</h3><div>Most biosensors achieved femtomolar to attomolar detection limits (LODs), reaching the required sensitivity limits for germline and somatic mutations respectively These LODs were achieved primarily via signal amplification strategies using redox mediators, nanomaterials, and strand displacement assays. Target amplification was less common and did not demonstrate consistent superiority. Specificity was enhanced through modified probes, enzymatic cleavage, magnetic separation, and blocking agents, though assessment methods varied. In general, covalently immobilized probes improved stability and sensitivity. However, most assays were validated in buffer with synthetic DNA, limiting clinical relevance.</div></div><div><h3>Conclusions</h3><div>With respect to their performance, electrochemical biosensors exhibit considerable potential for clinical integration. In particular when integrating different signal amplification strategies, such as a synergistic approach of redox mediators and nanocomposites, or combining target and signal amplification to reach the required sensitivity limits. Nonetheless, their clinical integration requires evaluation in complex biological samples, more thorough selectivity studies, and rigorous validation on patient samples. When standard validation assays can be provided and performed, electrochemical biosensing assays hold great promise for the detection of cancer DNA biomarkers in liquid biopsies.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129488"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}