Talanta最新文献

{"title":"Efficient analysis of n-alkanes by high-pressure photoionization-induced NO₂⁺ chemical ionization time-of-flight mass spectrometry.","authors":"Xuefeng Sun, Bo Li, Guolong Wang, Yifan Ding, Anshan Xiao","doi":"10.1016/j.talanta.2026.130161","DOIUrl":"10.1016/j.talanta.2026.130161","url":null,"abstract":"<p><p>Normal alkanes (n-alkanes) are important constituents of anthropogenic volatile organic compounds (VOCs) and petroleum products. Their rapid, sensitive, and selective detection is crucial for environmental monitoring and industrial process control. Conventional GC-based methods are time-consuming, while existing direct MS techniques often suffer from low sensitivity or severe fragmentation. In this study, we introduce a novel high-pressure photon ionization-NO₂⁺ chemical ionization time-of-flight mass spectrometry (HPPI-PNO₂CI-TOFMS) platform for the efficient analysis of C₄-C₁₁ n-alkanes. A vacuum ultraviolet (VUV) lamp is used to photoionize NO₂ reagent gas at pressures of approximately 600-700 Pa, generating NO₂⁺ reactant ions that undergo hydride-transfer reactions with n-alkanes to produce predominantly [M - H]⁺ ions with minimal fragmentation, with [M - H]⁺ branching ratios exceeding 90-96% for C₄-C₁₁ alkanes. Under optimized conditions (1 V, ∼660 Pa), limits of detection (LODs) reach ∼8.6 ppbv for n-butane and 0.21-2.57 ppbv for C₅-C₁₁ n-alkanes with 5 s integration times. The method was successfully applied to refinery gas samples, facilitating the selective and rapid quantification of n-alkanes against complex VOC backgrounds. However, this method does not detect C₁-C₃ n-alkanes and cannot distinguish n-alkanes from their branched or cyclic isomers by mass spectrometry alone. Nevertheless, the HPPI-PNO₂CI-TOFMS thus provides a robust, real-time analytical tool characterized by high sensitivity, clean spectra, and minimal fragmentation, making it well suitable for environmental monitoring, emissions tracking, and industrial process control.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130161"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"¹O₂-driven photobleaching pathways of xanthene dyes: A comparative study via LC-QTOF-MS/MS analysis and FT-IR spectroscopy.","authors":"Simone Bassini, Victoria Beltran, Nick Sleegers, Andrea Marchetti, Koen Janssens, Alexander L N van Nuijs, Karolien De Wael","doi":"10.1016/j.talanta.2026.130176","DOIUrl":"10.1016/j.talanta.2026.130176","url":null,"abstract":"<p><p>Singlet oxygen (¹O₂)-driven photodegradation is a key factor that controls the stability of xanthene dyes and a mechanism of their photobleaching is still lacking. Here, we report a comparative photodegradation study of five xanthene dyes (i.e., erythrosin B, eosin Y, fluorescein, phloxine B and rose bengal) under illumination conditions maximizing ¹O₂ generation. Time-resolved degradation was analyzed mainly by LC-QTOF-MS and FTIR spectroscopy, enabling identification of major intermediates and allowing the description of a general degradation mechanism. Despite the different molecular structures, only two different pathways were identified: i) all dyes were consecutively oxidized, yielding a major product with partial cleavage of the tricyclic xanthene core; ii) dyes lacking chlorine substitution on the peripheral benzoate ring presented a faster route, leading to complete chromophore breakdown and formation of low molecular weight oxidation products. These results show a relationship between structure and degradation pathway and provide important insights relevant to design, lifetime and conservation of xanthene-based dyes.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130176"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"X-ray fluorescence analysis of contemporary metal sculptures.","authors":"Leonardo S G Teixeira, Gianni Gallello, Miguel de la Guardia","doi":"10.1016/j.talanta.2026.130167","DOIUrl":"10.1016/j.talanta.2026.130167","url":null,"abstract":"<p><p>A portable X-ray fluorescence spectrometer (pXRF) has been employed to study the composition of contemporary metal sculptures. The equipment calibration was verified against a reference material and by using circulating coins with compositions declared by central banks. It was shown that coins can serve as a cheaper alternative for verifying the accuracy of XRF measurements. The analyses of 14 sculptures revealed that the same object can exhibit parts with markedly different compositions, particularly when these parts are independent and perform different functions within the artwork. On the other hand, a tendency towards compositional homogeneity was observed across the main body of the same artwork, consistent with the use of a single metal alloy in the sculpture's manufacture. Furthermore, it was not possible to establish unequivocal compositional characteristics for works attributed to the same artist, and it was found that certified limited-edition sculptures exhibited high-quality bronze, whereas other works did not adhere to a specific metal-alloy standard, reflecting different manufacturing processes. It has been confirmed that compositional analysis of sculptures using pXRF can be important for the certification and authentication of these artworks, as well as for evaluating material quality and potential restorations.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130167"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Competitive kinetic mechanisms in one-pot isothermal amplification-CRISPR systems: From model construction to performance evaluation.","authors":"Hao Jiang, Junyuan Yang, Anyi Li, Di Mou, Yulin Deng, Xuefei Lv","doi":"10.1016/j.talanta.2026.130184","DOIUrl":"10.1016/j.talanta.2026.130184","url":null,"abstract":"<p><p>The urgent demand for integrating real-time molecular diagnosis with isothermal amplification and CRISPR-based detection has underscored the critical need for streamlined, single-tube one-pot methodologies. However, such integration is often hindered by temporal incompatibilities, including premature activation of early amplification products by CRISPR components, which leads to primer degradation and reduced amplification efficiency. To address this challenge, we established a dedicated quantitative competitive kinetic framework for enzyme-free isothermal amplification-CRISPR one-pot systems, using hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) as representative models. Two core inhibitory mechanisms were identified: \"pre-activation-degradation inhibition\", in which early CRISPR activation degrades amplification intermediates, and \"substrate competition inhibition\" in which hairpin probes compete with reporter probes for CRISPR trans-cleavage. Corresponding kinetic equations were derived to describe these interactions quantitatively. Systematic experimental validation of key parameters such as DNA activator concentration, reporter probe concentration, hairpin probe concentration, and ribonucleoprotein (RNP) complex concentration, confirmed the reliability and predictive accuracy of the proposed models. These results provide mechanistic insights into the factors governing one-pot HCR/CHA-CRISPR coupling and identify conditions that optimize assay performance. Overall, this study offers a theoretical foundation and experimental guidance for probe design, reaction condition optimization, and sensitivity enhancement in enzyme-free isothermal amplification-CRISPR one-pot platforms, and provides a general reference for the rational integration of isothermal amplification and CRISPR-based detection.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130184"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to' MXene/zinc ion embedded agar/sodium alginate hydrogel for rapid and efffcient sterilization with photothermal and chemical synergetic therapy' [Talanta 266 (2024) 125101-125109/214574].","authors":"Yuwei Hu, Qin Zeng, Yifan Hu, Jinyun He, Heye Wang, Chunyan Deng, Dai Li","doi":"10.1016/j.talanta.2026.130024","DOIUrl":"10.1016/j.talanta.2026.130024","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130024"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Radiocarbon-based direct liquid scintillation counting method to quantify biogenic carbon and fossil-derived synthetic adulterants in commercial essential oils\" [Talanta 296 (2026) 129583].","authors":"K Arya Krishnan, S Bharath, N Karunakara","doi":"10.1016/j.talanta.2026.130127","DOIUrl":"10.1016/j.talanta.2026.130127","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130127"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A thiol-tolerant Au-Se anchored fluorescent aptamer nanosensor for GP73 detection with anti-interference validation and proof-of-concept serum/cellular imaging.","authors":"Lailin Fu, Congcong Xu, Guowen An, Lingyun Wang, Peng Li, Mei Lv, Xiaofei Zhang","doi":"10.1016/j.talanta.2026.130149","DOIUrl":"10.1016/j.talanta.2026.130149","url":null,"abstract":"<p><p>Golgi protein 73 (GP73) is a clinically relevant biomarker associated with hepatocellular carcinoma (HCC). However, fluorescence-based quantification and intracellular imaging are susceptible to interference and signal instability in thiol-rich biological environments. Herein, we report a fluorescent aptamer nanosensor based on Au-Se anchoring, which integrates a mechanistically guided anti-interference design with a calibratable, buffer-based quantitative workflow for GP73 detection. The sensing interface translates GP73 recognition into fluorescence recovery via target-induced aptamer conformational switching and increased fluorophore-gold nanoparticle distance, while the Au-Se bond mitigates thiol-driven false activation. Under optimized conditions, the assay shows a linear response in the range of 0-800 ng/mL with a detection limit of 1.51 ng/mL. Stability and tolerance studies demonstrate its robust performance across a pH range of 6.2-8.0 and in the presence of physiologically relevant concentrations of glutathione. Notably, in 5 mM glutathione, the signal increases by less than 1.5-fold compared to the untreated control, and its performance is significantly superior to that of the conventional Au-S anchored probe. Specificity experiments confirm that the probe responds significantly only to GP73, showing negligible responses to a panel of non-target proteins and a scrambled aptamer control. Preliminary serum experiments further demonstrate feasibility in a 10× diluted human serum matrix, where the probe preliminarily differentiates GP73-positive HCC patient sera from sera of other cancer patients using a ΔF strategy to reduce serum background fluorescence; however, we emphasize that the cohort size is limited and larger clinical validation is required for definitive clinical claims. Cellular experiments reveal GP73-correlated fluorescence contrast between HepG2 and normal liver (THLE-2) cells, and the thiol-depletion control (N-ethylmaleimide) supports that intracellular signal activation is thiol-independent. In summary, this work provides a robust Au-Se-stabilized nanosensor for quantitative GP73 readout in buffer and cellular imaging under thiol-rich conditions. The control experiments clearly define its specificity and anti-interference advantages, delineating key practical operating boundaries and providing a foundation for future expanded validation in complex clinical matrices.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130149"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIR-responsive MnO₂-functionalized mesoporous silica hydrogel: Fabrication and antibacterial activity evaluation.","authors":"Xue Yang, Yu Si, Shasha Jiang, Meng Zhuo, Tong Ren, Zijun Han, Xuetong Zhao, Xueyan Hou, Yongli Shi","doi":"10.1016/j.talanta.2026.130186","DOIUrl":"10.1016/j.talanta.2026.130186","url":null,"abstract":"<p><p>In this study, an 808 nm near-infrared (NIR)-responsive hydrogel (NOR-MSN@MnO₂-gel) was developed for the treatment of bacteria-infected wounds. The norfloxacin (NOR)-loaded manganese dioxide-modified mesoporous silica (NOR-MSN@MnO₂) nanoparticles were selected as a photothermal material and encapsulated in the furfuryl amine-modified hyaluronic acid hydrogel (HA-FA^gel). Studies found that MSN@MnO₂-gel exhibited a three-dimensional porous network structure, favorable injectability, excellent water absorption & retention capacity, and desirable photothermal conversion efficiency. Benefiting from pH/NIR dual-responsive characteristics, NOR-MSN@MnO₂-gel realized controllable NOR release and achieved efficient elimination of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) through synergistic photothermal therapy (PTT) and chemotherapy. In vivo infected wound studies demonstrated that NOR-MSN@MnO₂-gel combined with NIR irradiation (NOR-MSN@MnO₂-gel + NIR) could effectively inhibit bacterial growth, alleviate inflammation, accelerate re-epithelialization, and promote collagen deposition & angiogenesis, while maintaining satisfactory biological safety. Collectively, the NOR-MSN@MnO₂-gel + NIR regimen provides a promising strategy for clinical treatment of bacterial infected wounds.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130186"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-aided Cu₃(HHTP)₂/COOH-MWCNTs electrochemical sensing platform for simultaneous detection of dopamine and uric acid in human serum.","authors":"Zihao Zhao, Jianlong Li, Jinmi Zhang, Qiang Li, Sheng Li, Yingbo Shi, Xiaoli Xiong","doi":"10.1016/j.talanta.2026.130163","DOIUrl":"10.1016/j.talanta.2026.130163","url":null,"abstract":"<p><p>The simultaneous determination of dopamine and uric acid in complicated biological matrices remains challenging due to overlapping electrochemical signals and interference from coexisting species. Herein, a machine learning-assisted electrochemical sensor based on a Cu₃(HHTP)₂/COOH-MWCNTs hybrid is developed for the simultaneous determination of these analytes. Benefiting from the unique π-π stacking and π-d conjugation characteristics of Cu₃(HHTP)₂, as well as the excellent coordination effect between COOH-MWCNTs and Cu²⁺ and the good dispersibility of carbon nanotubes themselves, the composite material exhibits more prominent surface activity, significantly boosting the exposure of active sites and accelerating the electron transfer kinetics at the electrode-electrolyte interface. On this basis, we constructed an electrochemical sensing platform for the simultaneous detection of DA and UA in human serum samples. The sensor show excellent performance with dual linear ranges of 0.5-15 and 15-80 μM for DA, and 1-200 and 200-350 μM for UA, achieving low LODs of 0.039 μM and 0.083 μM, respectively (S/N = 3). Furthermore, machine learning (ML) algorithms were employed to optimize electrochemical signal processing, enabling more accurate and robust analysis of complex data. The proposed approach achieved successful application in the simultaneous determination of DA and UA in human serum matrix samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130163"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-assisted molecularly imprinted fluorescent hydrogel microneedles for on-site screening of patulin on fruit surfaces.","authors":"Keyu Sun, Ziwuzhen Wang, Shuhong Ye","doi":"10.1016/j.talanta.2026.130590","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130590","url":null,"abstract":"<p><p>Patulin (PAT) is a hazardous mycotoxin frequently detected in fruits and fruit-derived products, requiring rapid, portable, and visual strategies for on-site food safety screening. Herein, a gelatin methacryloyl (GelMA)/quantum dot-loaded molecularly imprinted polymer (QDs@MIP) hydrogel microneedle platform was developed by integrating molecular imprinting-based recognition, quantum dot fluorescence transduction, and microneedle-assisted surface sampling. QDs@MIP particles, prepared using QDs as fluorescent probes and molecularly imprinted polymers as selective recognition layers, were embedded into GelMA microneedles. Multiscale characterization confirmed successful QDs@MIP construction and preservation of red fluorescence after imprinting and microneedle encapsulation. The obtained microneedles exhibited well-defined arrays, uniform fluorescence distribution, rapid swelling, elastic gel-network behavior, and reliable compressive resistance, enabling conformal attachment and in situ sampling on fruit surfaces. Under optimized conditions, PAT binding and enrichment were accompanied by fluorescence attenuation at approximately 662 nm and a visible color transition under 365 nm UV-vis irradiation. Smartphone-assisted red-green-blue (RGB) analysis converted the fluorescence variation into quantitative signals, with the R/G ratio serving as the analytical parameter for visual PAT determination, achieving a limit of detection (LOD) of 0.009 μg L^-1 and a limit of quantification (LOQ) of 0.1 μg L^-1. The method provided recoveries of 82.0-112.0% across multiple fruit matrices and showed strong agreement with UPLC-MS/MS analysis. Moreover, the microneedles exhibited enzymatic and soil degradation potential and no obvious in vitro cytotoxicity. This work provides a portable, sustainable, and user-friendly strategy for rapid on-site screening of PAT in fruits.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt C","pages":"130590"},"PeriodicalIF":6.7,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}