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A robust polymetallic-coated sheathless interface with high acid and alkali resistance for coupling capillary electrophoresis with mass spectrometry. 用于毛细管电泳与质谱联用的坚固耐用、耐酸碱的多金属涂层无护套接口。
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-10-14 DOI: 10.1016/j.talanta.2024.127045
Xiaozhong Hu, Wenqing Gao, Rong Liu, Chen Tang, Huanming Wu, Jiancheng Yu, Yuheng Wang, Keqi Tang
{"title":"A robust polymetallic-coated sheathless interface with high acid and alkali resistance for coupling capillary electrophoresis with mass spectrometry.","authors":"Xiaozhong Hu, Wenqing Gao, Rong Liu, Chen Tang, Huanming Wu, Jiancheng Yu, Yuheng Wang, Keqi Tang","doi":"10.1016/j.talanta.2024.127045","DOIUrl":"10.1016/j.talanta.2024.127045","url":null,"abstract":"<p><p>A robust interface for coupling capillary electrophoresis (CE) to mass spectrometry (MS) was critical to maintain high separation efficiency of CE while achieving high sensitivity of MS. Current interfaces often suffer from problems such as reproducibility and ruggedness. For this purpose, a new polymetallic-coated sheathless interface was developed for the coupling of CE with MS. The electrical contact of the interface was achieved by etching one end of the fused silica capillary into a tapered tip using hydrofluoric acid (HF) solution, and then depositing a thin layer of chromium followed by a layer of platinum on it via physical vapor deposition technique. The performance of the new sheathless interface was systematically evaluated for the effect of flow rate and electrospray ionization (ESI) voltage on MS signal intensity, as well as the sample loading volume on CE separation efficiency and repeatability by using peptide standards and tryptic digest of bovine serum albumin (BSA). The interface was capable of generating stable electrospray even at ultra-low flow rate of 12.2 nL/min. In addition, the acid and alkali resistance of the polymetallic-coated emitter was tested by immersing it into 1 M HCL and 1 M NaOH solution, respectively. The results showed that polymetallic coating was still intact even after continuous immersion in the alkaline solution for 8 days (192 h) and a longer period in the acidic solution, indicating its excellent chemical stability. All the experimental results indicated that the sheathless interface fabricated by the new method in this study was robust and stable, making it promising for both sensitive and robust CE-MS sample analysis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127045"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A selective mitochondria-targeted fluorescent probe for imaging cysteine in drug-induced liver injury. 一种选择性线粒体靶向荧光探针,用于成像药物诱导肝损伤中的半胱氨酸。
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-10-16 DOI: 10.1016/j.talanta.2024.127056
Guixin Qin, Lingli Gao, Nan Yin, Mingxiu Wang, Yuting Wang, Jiali Tang, Jianhua Gong, Qingling Xu
{"title":"A selective mitochondria-targeted fluorescent probe for imaging cysteine in drug-induced liver injury.","authors":"Guixin Qin, Lingli Gao, Nan Yin, Mingxiu Wang, Yuting Wang, Jiali Tang, Jianhua Gong, Qingling Xu","doi":"10.1016/j.talanta.2024.127056","DOIUrl":"10.1016/j.talanta.2024.127056","url":null,"abstract":"<p><p>Cysteine (Cys) is involved in many physiological processes. It's challenging to detect Cys selectively as it has similar chemical structure with other biothiols such as homocysteine (Hcy) and glutathione (GSH). In this work, a novel fluorescence probe toward mitochondrial cysteine, HPXI-6C, has been developed by employing carbonate as a new recognizing unit and hemicyanine as a chromophore. HPXI-6C exhibits a high selectivity to Cys over hydrogen sulfide, homocysteine and glutathione. The limit of detection toward Cys was determined to be 42 nM. HPXI-6C can localize in mitochondria and produce strong fluorescence peaked at 725 nm in response to Cys in tumor cells. The uptake and generation pathways of Cys in acetaminophen hepatotoxicity cells was revealed by using HPXI-6C. HPXI-6C has been successfully applied in imaging of Cys in drug-induced liver injury in vivo. The research demonstrated that HPXI-6C is powerful in monitoring Cys and is conducive to the early diagnosis of drug-induced liver injury diseases.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127056"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Air plasma fast-induced defect-enriched carbon cloth for simultaneous electrochemical detection of dopamine and uric acid. 用于同时电化学检测多巴胺和尿酸的空气等离子体快速诱导缺陷富集碳布。
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-10-16 DOI: 10.1016/j.talanta.2024.127048
Jun Wang, Jing Ren, Yutong An, Xinyue Xu, Shiqi Yin, Liantao Xin, Hui Zhang, Qingxian Yu, Tianrong Zhan
{"title":"Air plasma fast-induced defect-enriched carbon cloth for simultaneous electrochemical detection of dopamine and uric acid.","authors":"Jun Wang, Jing Ren, Yutong An, Xinyue Xu, Shiqi Yin, Liantao Xin, Hui Zhang, Qingxian Yu, Tianrong Zhan","doi":"10.1016/j.talanta.2024.127048","DOIUrl":"10.1016/j.talanta.2024.127048","url":null,"abstract":"<p><p>Herein, we report a fast (10 min) and simple surface treatment of pure carbon cloth (CC) only by an air plasma. The structural characterizations indicate that the air plasma process brings out higher rugosity, more defects, and increased oxygen-related groups on CC surfaces than Ar- or N<sub>2</sub>-plasma, which can offer abundant capture sites, large electroactive area, and superhydrophilic interface for analytes. As a result, the air-treated CC (CC-P<sub>Air</sub>) electrode achieves a pronounced improvement of electrocatalytic activity for the [Fe(CN)<sub>6</sub>]<sup>3-</sup>/[Fe(CN)<sub>6</sub>]<sup>4-</sup> probe evidenced by increased peak currents, decreased peak-to-peak separation, and the lowered resistance of charge transfer. It is also demonstrated that the self-supported CC-P<sub>Air</sub> electrode possesses excellent sensing performance toward dopamine (DA) and uric acid (UA). The feasibility of the simultaneous electrochemical detection of DA and UA can be verified by their large peak potential gap (∼112 mV) for differential pulse voltammetry. The chronoamperometric sensor yields wide linear ranges of 0.05-100 μM for DA and 0.5-300 μM for UA. The corresponding detection limits are estimated to be 2.6 and 20.4 nM for DA and UA, respectively. The sensor also displays satisfactory selectivity, stability, and reproducibility. Due to good flexibility, the CC-P<sub>Air</sub> electrode presents great potential for manufacturing wearable and soft electronics for human health monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127048"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integration WGCNA with LC-MS data for evaluating the processing status and transformation rules of Ligustri Lucidi Fructus: A novel strategy for evaluating the processing technology of traditional Chinese medicines. 将WGCNA与LC-MS数据相结合,评价枸杞子的加工状态和转化规律:一种评估中药加工技术的新策略
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-10-10 DOI: 10.1016/j.talanta.2024.127029
Xuemei Zhang, Jinyan Li, Lin Zhang, Xiaolin Wu, Yijun Wang, Lele Zhang, Ying Zhou, Lifeng Han, Liming Wang, Erwei Liu
{"title":"Integration WGCNA with LC-MS data for evaluating the processing status and transformation rules of Ligustri Lucidi Fructus: A novel strategy for evaluating the processing technology of traditional Chinese medicines.","authors":"Xuemei Zhang, Jinyan Li, Lin Zhang, Xiaolin Wu, Yijun Wang, Lele Zhang, Ying Zhou, Lifeng Han, Liming Wang, Erwei Liu","doi":"10.1016/j.talanta.2024.127029","DOIUrl":"10.1016/j.talanta.2024.127029","url":null,"abstract":"<p><p>Ligustri Lucidi Fructus (LLF) is a traditional Chinese medicine (TCM) to treat hepatopathy and osteopathy. Wine-processed LLF (WLLF) was much more widely used than raw LLF (RLLF) in clinical practice, however, there is no consensus on processing time. To investigate the processing status of WLLF and transformation rules during processing, a UHPLC-Q-Orbitrap-MS method combined with data-independent acquisition (DIA) mode was firstly established and 227 compounds were identified or tentatively identified. Subsequently, a novel strategy using integration weighted gene co-expression network analysis (WGCNA) with LC-MS data was proposed. A total of 73 differential metabolites were screened out between RLLF and WLLF (wine steaming for 18 h). Meanwhile, the concentration of 11 differential compounds for WLLF was quantified. Finally, correlations between compounds were analyzed by WGCNA and the top five compounds negatively correlated with salidroside were validated, revealing that G13, specnuezhenide, oleuropein, acteoside, and neonuzhenide could be transformed into salidroside and its analogues during processing, respectively. The results indicated that our proposed strategy could be effectively employed to evaluate the processing status of TCMs.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127029"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial lipidomic profiles of atherosclerotic plaques: A mass spectrometry imaging study. 动脉粥样硬化斑块的空间脂质体特征:质谱成像研究
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-28 DOI: 10.1016/j.talanta.2024.126954
Sphamandla Ntshangase, Shazia Khan, Louise Bezuidenhout, Taťána Gazárková, Jakub Kaczynski, Stephanie Sellers, Nicholas Jw Rattray, David E Newby, Patrick Wf Hadoke, Ruth Andrew
{"title":"Spatial lipidomic profiles of atherosclerotic plaques: A mass spectrometry imaging study.","authors":"Sphamandla Ntshangase, Shazia Khan, Louise Bezuidenhout, Taťána Gazárková, Jakub Kaczynski, Stephanie Sellers, Nicholas Jw Rattray, David E Newby, Patrick Wf Hadoke, Ruth Andrew","doi":"10.1016/j.talanta.2024.126954","DOIUrl":"10.1016/j.talanta.2024.126954","url":null,"abstract":"<p><p>Lipids contribute to atherosclerotic cardiovascular disease but their roles are not fully understood. Spatial lipid composition of atherosclerotic plaques was compared between species focusing on aortic plaques from New Zealand White rabbits and carotid plaques from humans (n = 3), using matrix-assisted laser desorption/ionization mass spectrometry imaging. Histologically discriminant lipids within plaque features (neointima and media in rabbits, and lipid-necrotic core and fibrous cap/tissue in humans) included sphingomyelins, phosphatidylcholines, and cholesteryl esters. There were 67 differential lipids between rabbit plaque features and 199 differential lipids in human, each with variable importance in projection score ≥1.0 and p < 0.05. The lipid profile of plaques in the rabbit model closely mimicked that of human plaques and two key pathways (impact value ≥ 0.1), sphingolipid and glycerophospholipid metabolism, were disrupted by atherosclerosis in both species. Thus, mass spectrometry imaging of spatial biomarkers offers valuable insights into atherosclerosis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"126954"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to 'Four-dimensional trapped ion mobility spectrometry proteomics reveals circulating extracellular vesicles encapsulated drivers of nasopharyngeal carcinoma distant dissemination' [Talanta 282, 1 January 2025, 126907]. 四维阱式离子迁移谱蛋白质组学揭示循环细胞外囊泡包裹鼻咽癌远处扩散的驱动因素》[Talanta 282,2025 年 1 月 1 日,126907]的更正。
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-10-16 DOI: 10.1016/j.talanta.2024.127015
Pingan Lu, Liu Yang, Weiling Chen, Kaiguo Li, Xuxia Chen, Song Qu
{"title":"Corrigendum to 'Four-dimensional trapped ion mobility spectrometry proteomics reveals circulating extracellular vesicles encapsulated drivers of nasopharyngeal carcinoma distant dissemination' [Talanta 282, 1 January 2025, 126907].","authors":"Pingan Lu, Liu Yang, Weiling Chen, Kaiguo Li, Xuxia Chen, Song Qu","doi":"10.1016/j.talanta.2024.127015","DOIUrl":"10.1016/j.talanta.2024.127015","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"127015"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
1-(o-Tolyl)thiourea-based deep eutectic solvent as a stationary phase in flow injection analysis system for mercury and copper determination in edible oils. 以 1-(邻甲苯基)硫脲为基础的深共晶溶剂作为固定相,在流动注射分析系统中测定食用油中的汞和铜。
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-10-19 DOI: 10.1016/j.talanta.2024.127079
Andrey Shishov, Ulyana Markova, Valeriia Mulloyarova, Peter Tolstoy, Natalya Shkaeva, Dmitry Kosyakov, Nipu Kumar Das, Tamal Banerjee
{"title":"1-(o-Tolyl)thiourea-based deep eutectic solvent as a stationary phase in flow injection analysis system for mercury and copper determination in edible oils.","authors":"Andrey Shishov, Ulyana Markova, Valeriia Mulloyarova, Peter Tolstoy, Natalya Shkaeva, Dmitry Kosyakov, Nipu Kumar Das, Tamal Banerjee","doi":"10.1016/j.talanta.2024.127079","DOIUrl":"10.1016/j.talanta.2024.127079","url":null,"abstract":"<p><p>In this work, a novel deep eutectic solvent (DES) composed of thymol and 1-(o Tolyl)thiourea 9/1 (mol) is presented for the first time. This DES has not been described in the literature. This DES was first used as a stationary phase in an extraction column integrated into a flow injection analysis system for the simultaneous determination of mercury and copper in edible oils. The automated approach involves passing an aqueous sample solution obtained after microwave mineralization through a microcolumn of DES retained on polytetrafluoroethylene. This leading to the extraction and concentration of the analytes. The metals are then eluted with an aqueous thiourea solution for subsequent analysis by inductively coupled plasma-optical emission spectrometry. The limits of detection (LODs) for mercury and copper were 3 μg L⁻<sup>1</sup> and 2.5 μg L⁻<sup>1</sup>, respectively. The approach demonstrated high accuracy. Relative standard deviations (RSD) for repeatability and inter-day reproducibility ranged from 3 % to 11 %. Extraction recovery of both metals exceeded 95 %, indicating the high efficiency of the DES-based extraction process. Environmental assessment using the AGREEprep method yielded a favorable environmental index of 0.54, highlighting the robustness of the approach. This novel use of DES as a stationary phase in flow injection analysis system provides a robust, efficient and environmentally friendly approach to the determination of trace metals in edible oils. This method can also be applied to the analysis of other samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127079"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Towards the development of a portable device based on modified-voltammetric sensors for the detection of illicit drugs and seized samples. 开发基于改良伏安传感器的便携式设备,用于检测非法药物和缉获样品。
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-10-16 DOI: 10.1016/j.talanta.2024.127055
Xavier Cetó, Florina Maria Truta, Ana-Maria Dragan, Elena Rodríguez-Franch, Mihaela Tertis, Ángela Sánchez-Pereña, Sara Comellas-Tena, Cecilia Cristea, Manel Del Valle
{"title":"Towards the development of a portable device based on modified-voltammetric sensors for the detection of illicit drugs and seized samples.","authors":"Xavier Cetó, Florina Maria Truta, Ana-Maria Dragan, Elena Rodríguez-Franch, Mihaela Tertis, Ángela Sánchez-Pereña, Sara Comellas-Tena, Cecilia Cristea, Manel Del Valle","doi":"10.1016/j.talanta.2024.127055","DOIUrl":"10.1016/j.talanta.2024.127055","url":null,"abstract":"<p><p>Illicit drugs are a global burden, not only for society, but also for the various control authorities for which its rapid on-site detection remains a challenge. In this context, the potential of a voltammetric electronic tongue (ET) for the analysis of different drugs is evaluated herein. Concretely, the discrimination and identification of cocaine, heroin, 3,4-Methylenedioxymethamphetamine (MDMA), methamphetamine and ketamine in self-prepared and real samples were attempted. For its analysis, an array of three carbon-based screen-printed electrodes (SPE) was prepared, and their responses towards the different drugs and some of the more common cutting agents and adulterants were assessed by means of square wave voltammetry (SWV). To this aim, a tiny amount (ca. 3 mg) of the drug powder was added to the electrochemical cell containing phosphate buffer (pH 12), shaken, and measured directly without any other pre-treatment than its dilution. Next, to identify their characteristic fingerprint, obtained voltammograms were submitted to linear discriminant analysis (LDA), which allowed to correctly identify the different drugs regardless of the presence of the different cutting agents and other possible interfering compounds, or their concentration. Satisfactory results were obtained both for the synthetic and the \"street\" seized samples, with a classification rate of 100 % for the external test subset of the latter (n = 10).</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127055"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Achieving precise dual detection: One-tube reverse transcription-recombinase aided amplification (RT-RAA) combined with lateral flow strip (LFS) assay for RNA and DNA target genes from pepper mild mottle virus and Colletotrichum species in crude plant samples. 实现精确的双重检测:单管反转录-重组酶辅助扩增(RT-RAA)结合侧流条带(LFS)检测植物粗样品中辣椒轻度斑驳病病毒和壳斗孢菌的 RNA 和 DNA 目标基因。
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-18 DOI: 10.1016/j.talanta.2024.126908
Yuhao Cao, Dankan Yan, Huijie Zhou, Kelei Han, Qionglian Wan, Jiejun Peng, Hongying Zheng, Lin Lin, Fei Yan, Xuemei Song
{"title":"Achieving precise dual detection: One-tube reverse transcription-recombinase aided amplification (RT-RAA) combined with lateral flow strip (LFS) assay for RNA and DNA target genes from pepper mild mottle virus and Colletotrichum species in crude plant samples.","authors":"Yuhao Cao, Dankan Yan, Huijie Zhou, Kelei Han, Qionglian Wan, Jiejun Peng, Hongying Zheng, Lin Lin, Fei Yan, Xuemei Song","doi":"10.1016/j.talanta.2024.126908","DOIUrl":"10.1016/j.talanta.2024.126908","url":null,"abstract":"<p><p>Ensuring the detection sensitivity of both RNA-derived and DNA-derived target genes in a single reaction has posed a significant challenge for on-site detection of plant pathogens. This challenge was addressed by developing a one-tube dual RT-RAA assay combined with LFS for the rapid on-site detection of pepper mild mottle virus (PMMoV) and four Colletotrichum species causing anthracnose in Solanaceous crops. By testing four different combinations of primer groups, two combinations were precisely adjusted within the dual RT-RAA system to balance amplification efficiency and maintain consistent levels of amplification in crude plant samples. Utilizing commercially accessible small-scale equipment and following a streamlined optimization strategy, the assay achieved a limit of detection of 0.32 copies/μL of target genes in the reaction. Importantly, it demonstrated no cross-reactivity with other plant pathogens, thereby affirming the high sensitivity and specificity of the developed dual RT-RAA-LFS detection assay. Moreover, the entire process took only 25 min from sample collection to the visible presentation of results. The assay was validated with 60 field samples and 10 seed samples, producing results consistent with reverse transcription quantitative polymerase chain reaction (RT-qPCR). Notably, it successfully detected PMMoV in systemic leaves without visible symptoms three days post-inoculation, underscoring its effectiveness in early disease detection. This streamlined strategy offers a valuable approach for rapid, low-cost, and highly sensitive on-site simultaneous detection of RNA genome-contained PMMoV and DNA genome-contained Colletotrichum species.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126908"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescence of europium activated by molecular-like silver clusters for the detection of alkaline phosphatase activity. 用于检测碱性磷酸酶活性的分子样银簇激活的铕荧光。
IF 5.6 1区 化学
Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-16 DOI: 10.1016/j.talanta.2024.126892
Chen-Xi Zhao, Xiao-Xia Li, Yang Shu
{"title":"Fluorescence of europium activated by molecular-like silver clusters for the detection of alkaline phosphatase activity.","authors":"Chen-Xi Zhao, Xiao-Xia Li, Yang Shu","doi":"10.1016/j.talanta.2024.126892","DOIUrl":"10.1016/j.talanta.2024.126892","url":null,"abstract":"<p><p>Alkaline phosphatase (ALP) is abnormally expressed in some cancers and promotes the growth, metastasis, and invasion of cancer cells. The detection of ALP is of great significance for both pathological study and clinical detection. In this work, a europium (Eu)-based fluorescence detection sensor was prepared in a mild reaction condition. LaF<sub>3</sub>:Eu nanoparticles was mixed with ethylene imine polymer (PEI) and Ag<sup>+</sup> ions. PEI was used as stabilizer and reducing agent, and Ag<sup>+</sup> ions were reduced as molecular-like silver clusters (ML-Ag NCs). The fluorescence of LaF<sub>3</sub>:Eu nanoparticles was enhanced by ML-Ag NCs through energy transfer. When ascorbic acid 2-phosphate (AAP) was hydrolyzed to ascorbic acid (AA) in the presence of ALP, AA reduced Ag<sup>+</sup> ions to silver nanoparticles (Ag NPs) and quenched the fluorescence of LaF<sub>3</sub>:Eu/PEI/Ag. The activity of ALP was detected by measuring the fluorescence intensity of Eu<sup>3+</sup> at 618 nm. In the concentration range from 2.0 to 16.0 U/L, the fluorescence intensity ratio ((F<sub>0</sub>-F)/F<sub>0</sub>) had a linear relationship with the logarithm of ALP concentration. The limit of detection (LOD) was 1.3 U/L. Moreover, the ALP activity was detected successfully in cancer cells by this method. The sensing platform has application potential in the detection of ALP activity in biological systems.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126892"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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