The total antioxidant capacity of fruit was determined by TAMzyme pseudo-peroxidase activity colorimetry.

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2026-01-01 Epub Date: 2025-06-26 DOI:10.1016/j.talanta.2025.128517

Xinli Guo, Shuangshuang Yan, Nishan Jin, Wenbin Zhong, Dongying An, Murui Ying, Yutian Zhou, Yudong Wu, Xin Li, Yang Zhang

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引用次数: 0

Abstract

The total antioxidant capacity (TAC) of fruits is a key indicator for measuring their quality and nutritional value. Traditional detection methods are time-consuming and cumbersome, failing to meet the requirements for rapid detection. In this study, a novel triple metal organic framework nanozyme (TAMzyme) with superior peroxidase-like (POD-like) activity is constructed for colorimetric detection of TAC. Owing to the Fe, Co, Ni doping and unique long spindle structures, the TAMzyme possesses more surface active oxygen species, negative surface charges and rapid mass transport, thus resulting in higher catalytic activity and reaction rate. Thanks to the remarkable peroxidase-like activity, the antioxidant capacity of ascorbic acid (AA), cysteine (Cys), glutathione (Glu), and gallic acid (GA) was evaluated with a detection limit between 0.54 and 1.58 μM. Antioxidant capacity was quantified in μM Trolox Equivalent (TE), with Trolox as the standard reference compound. The sensor accurately determined the TAC in kiwi fruit (194.97 ± 2.73 μM TE) and oranges (205.10 ± 3.85 μM TE), aligning with results from a standardized ABTS kit. This study presents a rapid, cost-effective, and straightforward method for quantifying TAC in complex food matrices.

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采用TAMzyme伪过氧化物酶活性比色法测定果实的总抗氧化能力。

水果的总抗氧化能力（TAC）是衡量水果品质和营养价值的重要指标。传统的检测方法耗时长、操作繁琐，无法满足快速检测的要求。本研究构建了一种具有优异过氧化物酶样活性的新型三金属有机框架纳米酶（TAMzyme），用于TAC的比色检测。由于Fe、Co、Ni的掺杂和独特的长纺锤体结构，使TAMzyme具有更多的表面活性氧，表面负电荷和快速的质量传递，从而具有更高的催化活性和反应速率。由于抗坏血酸（AA）、半胱氨酸（Cys）、谷胱甘肽（Glu）和没食子酸（GA）具有显著的过氧化物酶样活性，检测限在0.54 ~ 1.58 μM之间。以Trolox为标准参比物，用μM Trolox当量（TE）测定抗氧化能力。该传感器可准确测定猕猴桃（194.97±2.73 μM TE）和橙子（205.10±3.85 μM TE）中的TAC，与标准化ABTS试剂盒的结果一致。本研究提出了一种快速、经济、直接的方法来定量复杂食品基质中的TAC。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.