Talanta最新文献

{"title":"Cathepsin B-induced cascade DNA-AuNP nanomachine for activated tumor theranostics.","authors":"Huihui Liu, Limei Xu, Ting Wang, Yingqi Liu, Jiajia Pan, Weiwei Xiong, Fenfen Zheng, Yemei Wang, Shasha Sun","doi":"10.1016/j.talanta.2025.128103","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128103","url":null,"abstract":"<p><p>Since targeted and efficient accumulation of nanoparticles into tumors is essential for accurate cancer theranostics, spatiotemporally controlling the aggregation of small nanoparticles (such as gold nanoparticles, AuNPs) in the tumor microenvironment holds significant promise for improving the diagnostic and therapeutic efficiency against tumors. Here, we introduce a cascade DNA-AuNP nanomachine (CNM) that can in situ magnify the protease-catalyzed peptide cleavage via DNA amplification machinery for cathepsin B (Cat B) activity imaging and Cat B-responsive photothermal therapy of tumors. The CNM is composed of a nanomediator formed by tethering a mediator DNA/peptide complex on AuNPs (DpAuNP) and a nanoeffector consisted of AuNPs and DNA modules (DNA-AuNP). In the cascade, Cat B-mediated peptide cleavage of mediator DNA/peptide complex on DpAuNPs initiates both the detachment of fluorescent DNA reporter from DNA-AuNPs for Cat B imaging and the aggregation of AuNPs for tumor photothermal therapy via toehold-mediated stand displacement (TMSD) reaction. Our results demonstrate that the CNM not only offers superior sensitivity and specificity for Cat B imaging, but also facilitates the activated aggregation of AuNPs for enhanced photothermal therapy of tumors. This CNM represents a Cat B-specific sense-and-treat paradigm for cancer theranostics.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128103"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-integrated ratiometric colorimetric aptasensor for visual detection of ampicillin residue in animal-derived food samples.","authors":"Reena K Sajwan, Pratima R Solanki","doi":"10.1016/j.talanta.2025.128057","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128057","url":null,"abstract":"<p><p>The antibiotic residue in food accelerates bacteria's resistance process that is responsible for serious health issues. A smartphone-integrated ratiometric colorimetric aptasensor has been developed for ampicillin (AMP) detection in milk and egg samples. The sensing phenomenon utilizes the optical and color-changing properties of gold nanoparticles (AuNPs) and specific aptamer with higher affinity towards AMP for its selective detection. The aptasensor shows linear response with AMP concentration 0.06-9.9 μM with a lower detection limit (LOD) of 45.0 nM. The smartphone-integrated color analyzer application was used to identify the solution's red, green, and blue (RGB) color intensity and calculate the G/R ratio of color intensities in the presence of AMP. Aptasensor show linear response with AMP concentration (0-6.6 μM), with a LOD of 53.0 nM. The sensor's results were further validated with liquid chromatography and mass spectroscopy techniques (LC-MS), showing that the developed sensor is suitable for food monitoring and safety applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128057"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper-manganese bimetallic oxide with excellent laccase-like activity for colorimetric detection of formaldehyde via the specific aldimine condensation reaction.","authors":"Haoyu Chen, Lin Chai, Jialing Gui, Yani Liu, Meiling Liu, Xiaoying Liu, Youyu Zhang, Shouzhuo Yao","doi":"10.1016/j.talanta.2025.128151","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128151","url":null,"abstract":"<p><p>Laccase, known as the \"green catalyst\", holds significant promise for applications in the textile industry and pollutant detection. However, the use of natural laccase is constrained by challenges associated with complex preparation and inherent instability. The emergence of nanozymes provides a pathway for developing laccase-like mimics. This study focuses on the synthesis of copper-manganese oxide (Cu-doped Mn₃O₄) nanoparticles, which demonstrate significant laccase-like activity through the oxidation coupling reaction between 2,4-dichlorophenol (2,4-DCP) and 4-aminoantipyrine (4-AAP) with observable colorimetric change and an obvious absorption peak at 505 nm. Different copper-manganese oxides were synthesized by varying the proportion of metal salts during the synthesis process. A series of studies have demonstrated that copper doping enhances laccase catalytic activity by increasing the oxygen vacancy. Moreover, the introduction of formaldehyde (FA) results in a decrease of the peak at 505 nm, which is attributed to a condensation reaction between the aldehyde and amino groups under neutral conditions. Based on the laccase-like properties of Cu-doped Mn₃O₄, FA detection is achievable within the range of 100 nM to 100 μM, with a low detection limit of 39.4 nM. This research not only presents a novel laccase mimic with a simple synthesis route but also establishes a colorimetric method for FA detection, representing significant progress in food safety applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128151"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemiluminescence lateral flow immunosensor using luminol-labeled silver nanoparticles for highly sensitive and quantitative detection of cardiac troponin I.","authors":"Jun Chen, Xuanxu Nan, Li Yang, Yue Cui","doi":"10.1016/j.talanta.2025.128159","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128159","url":null,"abstract":"<p><p>Cardiac troponin I (cTnI) is a highly specific biomarker of cardiomyocyte injury, released during cell disintegration and necrosis, and is the gold standard for diagnosing acute myocardial infarction (AMI). At the onset of AMI, cTnI appears in very low concentrations (pg/mL level), necessitating the development of highly sensitive and rapid detection sensors. In this study, an electrochemiluminescence lateral flow immunosensor (ECL-LFI) was designed using luminol-labeled silver nanoparticles (luminol@AgNPs) for the sensitive and quantitative detection of cTnI. A screen-printed electrode (SPE) was integrated beneath the nitrocellulose (NC) membrane with plastic plates of the same thickness applied on both sides of the SPE to ensure a smooth flow surface. Upon addition of cTnI and the luminol-H₂O₂ system, sandwich immune complexes formed by antibody-functionalized luminol@AgNPs on the strips generated electrochemiluminescent (ECL) signals. The ECL-LFI exhibited a broad linear detection range from 5 pg/mL to 100 ng/mL, with a detection limit as low as 1.6 pg/mL. Additionally, the results show excellent correlation with clinical tests, demonstrating that the ECL-LFI provides a promising point-of-care tool for the early diagnosis of AMI and other diseases.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128159"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical review of pesticides in aquatic environment: Current trends, environmental impacts, and advances in analytical extraction techniques.","authors":"Debasmita Datta, Bishwatma Biswas, Ayan Lodh, Vishal Kumar Parida, Sudha Goel","doi":"10.1016/j.talanta.2025.128094","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128094","url":null,"abstract":"<p><p>Pesticides are applied in agricultural fields to manage pests and diseases that threaten crop health and productivity. However, their presence in natural water systems is a significant concern due to their persistent composition and complex molecular structures. Additionally, their toxic and recalcitrant nature poses potential risks, leading to chronic health effects in humans. Typically detected in trace concentrations, pesticides present analytical challenges owing to their intricate chemical structures and diverse physical properties. Recent research highlights notable advancements in conventional pesticide extraction methods, aiming to develop eco-friendly and cost-effective techniques with high enrichment and recovery rates. This review begins by exploring the latest trends and ongoing research related to the occurrence and extraction of pesticides from various aquatic environments. The study then discusses the innovative extraction techniques currently employed for pesticide removal. Among liquid-phase microextraction (LPME) techniques, methods such as ionic liquid-based extraction (IL-LPME), deep eutectic solvent-based extraction (DES-LPME), air-assisted extraction, solidification of a floating organic drop (SFO), and ultrasound-assisted LPME are gaining attention due to their ease of handling, operational simplicity, cost-effectiveness, and environmental sustainability. In the, solid-phase extraction (SPE) field, researchers have increasingly utilized approaches like magnetic solid-phase extraction (MSPE), green sorbents, metal-organic framework (MOF) based extraction, cartridge-based SPE, and carbon nanotube-based SPE as the most widely adopted methods. These methods are preferred for their benefits, including efficient separation, rapid analysis, and environmentally sustainable practices. The latter sections of this review present a detailed comparative analysis of these extraction methods, evaluating critical parameters such as operational time, cost, chemical and energy consumption, and analytical accuracy.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128094"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional AuNRs/MXene/NFs -based SERS platform for determination of quetiapine in urine.","authors":"Jiamin Gong, Boran Zhang, Qinyi Li, Jiangli Shi, Xinling Liu, Yiping Wu, Ying Wen, Xiaoyu Guo, Haifeng Yang","doi":"10.1016/j.talanta.2025.128157","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128157","url":null,"abstract":"<p><p>Determination of antipsychotic drugs in humans is meaningful for both individualized therapy and therapeutic drug monitoring. Quetiapine (QTP), as one of the newly developed antipsychotic drugs, may pose risks to human health if used improperly. In this work, we synthesized a three-dimensional (3D) Au nanorods/MXene (Ti₃C₂T_x)/nickel foams (named as AuNRs/MXene/NFs) composite material for detecting the antipsychotic drugs QTP. The prepared negatively charged MXene was first assembled on the surface of the NFs through electrostatic interaction to form MXene/NFs, and then the prepared AuNRs were anchored on the MXene/NFs to obtain the final 3D AuNRs/MXene/NFs. By integrating the porous structure and magnetic properties of NFs, large specific surface area of MXene, and the plasmonic hot spots of Au nanorods, the AuNRs/MXene/NFs-based SERS platform can easily and sensitively detect QTP in human urine with the limit of detection of 1.71 × 10^-9 mol/L. The linearity was distinguished over the concentration range of QTP from 1 × 10^-7 to 1 × 10^-4 mol/L (calculated at 1030 cm^-1), with correlation coefficients (R²) of 0.9955. The presented AuNRs/MXene/NFs-based SERS strategy realizes QTP detection using SERS technology and provides a novel protocol for the therapeutic drug monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128157"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A smartphone sensing colorimetric detection of ascorbic acid in fruit samples based on Cu₂Fe(CN)₆@PVP nanozyme.","authors":"Xinyu Zhang, Xuan Zhou, Quan Wan, Taolei Sun, Guanbin Gao","doi":"10.1016/j.talanta.2025.128152","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128152","url":null,"abstract":"<p><p>Prussian blue analogue nanozymes (PBANZs) have been widely employed in the colorimetric detection of various organic small molecules due to their peroxidase-like activity. However, the practical applications of PBANZ are limited by its narrow operating pH range, insufficient catalytic activity, and poor stability. In this study, we synthesized a novel Cu₂Fe(CN)₆@PVP PBA nanozyme (Cu-PBANZ) using polyvinylpyrrolidone (PVP) as a capping ligand. This novel Cu-PBANZ not only exhibited enhanced peroxidase-like activity and improved stability under near-neutral conditions but also demonstrated higher sensitivity and a broader linear range in the detection of hydrogen peroxide (20-4000 μM, LOD 5 μM) and ascorbic acid (10-2000 μM, LOD 3.9 μM). Besides, a smartphone-based platform was developed for detecting AA content in fruits, achieving a detection limit of 7.8 μM. Both UV-Vis spectrophotometry and smartphone-based colorimetry were validated with recovery rates of 95.1 %-106.7 % and 94.8 %-108.9 %, respectively. This study proposed a promising biosensor incorporating Cu-PBANZ for rapid colorimetric detection of AA in fruits using a smartphone.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128152"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic recyclable metal-organic framework sheets grafted with curcumin for highly efficient adsorption and sensitive detection of fluoride.","authors":"Shisen Li, Yinghui Wang, Zhaopeng Yang, Ping Liu, Yanting Ren, Yue Li, Haiyan Jiang, Qingli Yang, Junghwan Kim, Yongchao Ma","doi":"10.1016/j.talanta.2025.128156","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128156","url":null,"abstract":"<p><p>The contents of fluoride in drinking water should be limited to a safe range. Hence, design and development of a simple and efficient technology for adsorption and detection of fluoride is urgently required. We presented a curcumin in situ grafted MOF magnetic material (Fe₃O₄/MOF-Cur) for dual-functionalized applications of fluoride detection and adsorption. Owing to the electrostatic interaction, ligand exchange at the metal sites and boron-fluorine complexation, the adsorption capacity of Fe₃O₄/MOF-Cur for fluoride is 99.92 mg/g, and the detection limit is 1.17 μM. The fluoride adsorption behavior on different active units was further visualized by theoretical calculations. Favorable stability (79.52 mg/g after 5 adsorption-desorption cycles and insignificant change of fluorescence intensity within 30 days), mild reaction conditions (pH = 3-5) and excellent anti-interference ability promote the application of Fe₃O₄/MOF-Cur in real samples. Finally, the paramagnetic properties (29 emu/g) allow for easy recovery from water. These findings deepen the understanding on the design and development of dual-functionalized MOF materials, and prove the application potential of Fe₃O₄/MOF-Cur for the remediation of fluoride-related environmental pollution.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128156"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Femtomolar-level detection of procalcitonin using a split aptamer-based fiber optic nanogold-linked sorbent assay for diagnosis of sepsis.","authors":"Yen-Ta Tseng, Yi-Hyuan Yu, Ying-Yi Yeh, Phuong Chi Mai, Tze-Ta Huang, Chun-Jen Huang, Lai-Kwan Chau, Yuh-Ling Chen","doi":"10.1016/j.talanta.2025.128150","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128150","url":null,"abstract":"<p><p>Sepsis requires very sensitive and rapid detection of biomarkers to guide antibiotic therapy to lower the patient mortality rate. Here, we report an ultrasensitive, rapid, and low-cost method for detection of the most popular sepsis biomarker, procalcitonin (PCT), based on a split aptamer-based fiber optic nanogold-linked sorbent assay. After successful development of a novel aptamer for PCT by SELEX, a pair of split aptamer fragments from the parent aptamer was predicted through molecular interaction simulation via free webservers. Then one fragment (AptF1) is modified on a sensor fiber and the other fragment (AptF2) is conjugated to a gold nanoparticle (AuNP@AptF2). The interactions among PCT and the two aptamer fragments lead to the formation of a AuNP@AptF2-PCT-AptF1 sandwich-like nanocomplex on the fiber core surface, leading to strong nanoplasmonic absorption near the fiber core surface. This method provides a linear response ranging from 500 fg/mL to 5.0 ng/mL and a detection limit of 50 fg/mL (3.9 fM). The method had been validated against the clinical accepted electrochemiluminescence immunoassay using clinical samples and very good correlation between the results by both methods was found. Therefore, this method offers a potential for point-of-care diagnosis of sepsis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128150"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-platinum nanozyme-mediated ratiometric fluorescent immunosensor with dual catalytic cascades for sensitive detection of fenitrothion.","authors":"Jie Liu, Hao Deng, Qing-Chun Yin, Min-Fu Wu, Lin Luo, Hong Wang, Zhen-Lin Xu, Bao-Zhu Jia","doi":"10.1016/j.talanta.2025.128162","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128162","url":null,"abstract":"<p><p>Organophosphorus pesticide residues pose significant threats to food safety and environmental health, and their monitoring methods demand sensitivity and anti-interference capability. In this study, we developed a novel ratiometric fluorescent immunosensor for fenitrothion (FN) detection by employing chitosan-modified platinum nanoparticles (Ch-Pt NPs) as catalytic nanozymes. Ch-Pt NPs exhibit oxidase-mimicking activity, enabling the oxidation of ascorbic acid (AA) and o-phenylenediamine (OPD) to produce dehydroascorbic acid (DHAA) and 2,3-diaminophenazine (DAP, Em = 568 nm), respectively. Then, DHAA further reacted with the remaining OPD to generate quinoxaline derivatives (DFQ, Em = 430 nm). These two products further form ratiometric fluorescent signals. Taking AA as a breakthrough point, the dual-catalytic cascade of natural enzymes and nanozymes was achieved by the combination with the alkaline phosphatase (ALP) -based enzyme-linked immunosorbent assay (ALP-ELISA). Compared with the conventional colorimetric ELISA, the proposed sensor demonstrated a nearly 20-fold enhancement in sensitivity (detection limit: 0.48 ng/mL) and achieved satisfactory recoveries of 80.0-108.3 % in spiked samples. The ratiometric fluorescent immunosensor demonstrates outstanding performance and holds great potential for extensive applications in the field of pesticide monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128162"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}