Talanta最新文献

{"title":"Corrigendum to \"Magnetic nanoparticles amplification-based terahertz and fluorescence dual-mode assays for efficient isolation and sensitive detection of circulating tumor cells\" [Talanta 298 (2026) 128894].","authors":"Yi Zhou, Mei Xin, Ling Liang, Fengxin Xie, Huiyan Tian, Weidong Jin, Yitong Ren, Jia Shi, Ke Yang, Xiang Yang","doi":"10.1016/j.talanta.2026.130463","DOIUrl":"10.1016/j.talanta.2026.130463","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130463"},"PeriodicalIF":6.7,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148786792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ce-doped ZnO upconversion nanoparticles for optical thermometry, antibacterial therapy, and cardiac biomarker detection.","authors":"Yuemei Li, Yongmei Li","doi":"10.1016/j.talanta.2026.130277","DOIUrl":"10.1016/j.talanta.2026.130277","url":null,"abstract":"<p><p>Lanthanide-doped upconversion nanoparticles (UCNPs) due to their unique optical properties are promising for applications ranging from bioimaging to biosensing. However, their practical implementation remains challenging owing to relatively low luminescence efficiency. Herein, we synthesize a series of Ce-doped Yb/Eu/ZnO, Yb/Er/ZnO and Yb/Tm/ZnO UCNPs via a hydrothermal method. Ce ions incorporation promotes anisotropic growth, which increases surface roughness and modulates the local coordination environment. Electrochemical impedance spectroscopy and Tafel plot analyses reveal that Ce doping significantly enhances charge separation and transfer kinetics. Ce ion doping markedly increases the emission intensities of Eu³⁺, Er³⁺ and Tm³⁺, attributed to the formation of Yb³⁺ dimers and efficient energy transfer under 980 nm excitation. Benefiting from these improvements, the Ce-doped ZnO UCNPs exhibit three distinct bio-applications: (i) superior low-temperature optical thermometry with a maximum relative sensitivity of 4.81% K^-1 at 80 K; (ii) excellent antibacterial activity against S. aureus and E. coli (up to 82.16% and 87.64% inhibition at 8 mol% Ce); and (iii) successful employment as luminescent probes in a multiplex immunoassay for the cardiac markers BNP (detection range 10-100 pg/mL) and ST2 (5-25 ng/mL), with high specificity and sensitivity. These findings underscore the potential of Ce-doped ZnO UCNPs as multifunctional platforms for optical thermometry, antibacterial therapy and biomedical diagnostics.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130277"},"PeriodicalIF":6.7,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148443569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral plasmonic Cu_2-xS quantum dots enable chirality-dependent fluorescent recognition of tryptophan enantiomers.","authors":"Meihan Guo, Ruiju Yang, Chenlei Gu, Lu Liu, Jian Wang, Shu Jun Zhen, Hong Yan Zou, Cheng Zhi Huang","doi":"10.1016/j.talanta.2026.130307","DOIUrl":"10.1016/j.talanta.2026.130307","url":null,"abstract":"<p><p>The accurate discrimination and quantification of chiral amino acids enantiomers have remained significant challenges in both chemistry and biology. In this study, we employed an etching method to synthesize two types of chiral plasmonic fluorescent Cu_2-xS quantum dots for efficient enantioselective L/D-tryptophan (Trp) enantiomers. Specifically, homochiral interactions between the Cu_2-xS and Trp enantiomers led to fluorescence enhancement, which could be attributed to the aggregation-induced emission (AIE) effect. In contrast, heterochiral interaction led to negligible fluorescence change. Leveraging these fluorescence variations, sensitive discrimination and detection of Trp enantiomers using L-Cu_2-xS with a limit of detection (LOD) of 0.3 μM for L-Trp, where the enantioselective fluorescence enhancement ratio reached up to 447. And the LOD was 0.1 μM for D-Trp using D-Cu_2-xS, where the enantioselective fluorescence enhancement ratio was 11.8. This research not only provides an effective strategy for the preparation of chiral plasmonic materials with strong emission properties but also offers a simple yet sensitive method for chiral recognition, thereby opening up new perspectives and opportunities in related research fields.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130307"},"PeriodicalIF":6.7,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148443626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-function antifouling/sterilization electrically neutral hydrogel mediated bipolar autoluminescencet coreactant-free electrochemiluminescent biosensor.","authors":"Luting Yan, Lei Tian, Zekun Ren, Chao Wang, Xia Sun, Yemin Guo, Maja Milojević-Rakić, Yanyan Zhang","doi":"10.1016/j.talanta.2026.130301","DOIUrl":"10.1016/j.talanta.2026.130301","url":null,"abstract":"<p><p>The scarcity of cathodic autoluminescencet restricts the development of coreactant-free electrochemiluminescent sensor for simultaneous detection of two targets. Herein, a novel coreactant-free electrochemiluminescent sensor was prepared based on Zr-DPA@TCPP MOF and AuAgNCs bimetallic nanoclusters served as a cathodic and anodic autoluminescencet emitter respectively. Significantly, this sensor system without any coreactants, effectively overcoming the simultaneous detection issue typically relying on different coreactants for cathode and anode luminescence. Meanwhile, complex matrix components can compromise the specificity and accuracy of the detection. Therefore, highly conductive and electrically neutral hydrogel was obtained to reduce interference from non-target substances. Poly (3,4-ethylenedioxythiophene): polystyrene sulphonate (PEDOT: PSS) was encapsulated in hydrogel to obtain PEDOT: PSS@hydrogel, besides, the crosslinking agents citronellal and TCPP provide a sterilization effect. Dual-function antifouling/sterilization electrically neutral hydrogel effectively addresses the main issues of complex matrix interference and secondary contamination. Under optimal conditions, the linear range was 1 × 10^-3 ∼ 1 × 10⁵ ng/L for Cd(II) and 1 ∼ 1 × 10⁸ CFU/mL for S. aureus. The biosensor achieves remarkably low limits of detection (LODs) at 1.71 pg/L and 1.36 CFU/mL for Cd(II) and S. aureus, respectively. This work offers a new perspective on the development of coreactant-free ECL technology with high accuracy and stability.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130301"},"PeriodicalIF":6.7,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical detection of cisplatin-DNA adduct formation using dielectrophoresis-based impedance spectroscopy.","authors":"Michihiko Nakano, Masafumi Inaba, Boonchai Techaumnat, Junya Suehiro","doi":"10.1016/j.talanta.2026.130268","DOIUrl":"10.1016/j.talanta.2026.130268","url":null,"abstract":"<p><p>Chemicals that form DNA adducts can be carcinogenic; therefore, their detection is essential for cancer research and genotoxicity screening. However, existing methods often require complex instrumentation or electrode modification, highlighting the need for simpler detection approaches. We aimed to develop a simple and rapid method for detecting DNA adduct formation using dielectrophoresis (DEP)-based impedance spectroscopy. Cisplatin was used as a model DNA-adduct-forming agent and incubated with DNA-labeled microbeads. Changes in DEP behavior were analyzed through crossover frequency measurements and impedance spectroscopy during frequency sweeps (200 kHz-10 MHz). Cisplatin treatment under conditions intended to promote DNA adduct formation was associated with a concentration-dependent decrease in the crossover frequency of DNA-labeled microbeads. During frequency sweeps, the conductance changed in the frequency region corresponding to the DEP transition, and the peak frequency derived from the conductance response showed strong agreement with the crossover-frequency trend. Quantitative detection was achieved over 0.05-0.5 mM, with a detection limit of 0.05 mM (50 μM). This DEP-based impedance approach offers a simple, cost-effective alternative to conventional methods that require electrode modification. Although the detection limit requires substantial improvement for future biological or clinical applications, the method provides a simple proof-of-concept platform for preliminary screening and methodological development.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130268"},"PeriodicalIF":6.7,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel targeted high-throughput workflow for serum chemical mixture profiling in population exposome-wide association studies.","authors":"Yan Wu, Linlin Chen, Jie Zhang, Lisong Chen, Shanshan Du, Rui Ding, Weimin Ye","doi":"10.1016/j.talanta.2026.130275","DOIUrl":"10.1016/j.talanta.2026.130275","url":null,"abstract":"<p><p>Simultaneous determination of environmental pollutants with complex compositions and wide-ranging acid dissociation constants in biological matrices presents a significant analytical challenge. This study developed a highly sensitive and broad-spectrum analytical method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). By employing phospholipid removal purification (PRP) technology and optimizing mobile phase pH conditions, we achieved simultaneous quantification of 230 environmental pollutants in serum. Comparative evaluation of sample pretreatment techniques revealed that low-temperature concentration caused preservative loss, while liquid-liquid extraction showed poor recovery for multiple compound classes. The PRP method was selected as the optimal approach due to its superior performance in reducing matrix effects compared to direct protein precipitation. Method validation demonstrated accurate quantification of all target analytes (relative standard deviation, RSD <15.1%), with 98.3% of compounds exhibiting satisfactory recoveries (80-120%). Analysis of serum samples from the Fuqing cohort participants detected 22 contaminants with detection frequencies >30%, among which PFHpA and PFOA were identified as risk factors for hyperuricemia. Notably, we provide the first evidence of a dose-response relationship between dehydroacetic acid exposure and hyperuricemia prevalence in a human population. This study established a robust solution for simultaneous screening of exposome-scale pollutants in complex biological samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130275"},"PeriodicalIF":6.7,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to \"Preparation and characterization of magnetic nanocomposite of Schiff base/silica/magnetite as a preconcentration phase for the trace determination of heavy metal ions in water, food and biological samples using atomic absorption spectrometry\" [Talanta 97 (2012) 87-95].","authors":"Hasan Bagheri, Abbas Afkhami, Mohammad Saber-Tehrani, Hosein Khoshsafar","doi":"10.1016/j.talanta.2026.130437","DOIUrl":"10.1016/j.talanta.2026.130437","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130437"},"PeriodicalIF":6.7,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148786849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymeric microneedle patches for rapid DNA extraction and molecular diagnosis of foliar pathogens of mung bean.","authors":"Asim Mushtaq, Barsha Poudel, Alina Karki, Pouria Azarikhah, Ahmed Saad, Adrian Dinsdale, Sonia Fiorito, Noel L Knight, Zahra Faraji Rad","doi":"10.1016/j.talanta.2026.130267","DOIUrl":"10.1016/j.talanta.2026.130267","url":null,"abstract":"<p><p>Global food security is threatened by crop losses due to plant diseases. Early detection of pathogens is essential for implementing effective management practices to mitigate the negative impacts of disease. On-site molecular diagnostic techniques have significantly advanced for rapid and accurate identification of pathogens, but DNA extraction remains a labour-intensive and time-consuming bottleneck. This study demonstrates the ability of polyvinyl alcohol/polyvinylpyrrolidone (PVA/PVP) microneedle patches to extract detectable amounts of bacterial and plant DNA from diseased leaves within 1 min. This included DNA of a gram-negative Pseudomonas savastanoi pv. phaseolicola (Psp) and a gram-positive Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) bacteria, as well as mung bean (Vigna radiata). These bacteria cause halo blight and tan spot of mung bean, respectively. The DNA extracted using microneedle patches was of adequate quality and quantity to identify pathogens using real-time quantitative and droplet digital PCR (ddPCR). For leaves expressing halo blight symptoms, Psp DNA was detected in all DNA templates from microneedle (n = 5) and conventional extractions (n = 5). Similarly, in a time course study, Psp inoculated samples at day 6 showed the same detection in templates collected with microneedles and conventionally extracted samples. Detection of Cff DNA was less frequent, suggesting an impact of the sampling or inoculation technique. This study supports ongoing development of microneedle-based extraction as a simple and cost-effective method that could be used to support diagnosis of plant diseases, facilitate rapid decision-making for disease management, enhance biosecurity responses and area-freedom datasets, and prevent crop losses.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130267"},"PeriodicalIF":6.7,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148454247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoelectroanalytical multiplexing of RNA methylation signatures to decode oncogenic point mutations in cancer cells.","authors":"Víctor Pérez-Ginés, Rebeca M Torrente-Rodríguez, Raquel Rejas-González, Ana Montero-Calle, María Pedrero, José M Pingarrón, Rodrigo Barderas, Susana Campuzano","doi":"10.1016/j.talanta.2026.130180","DOIUrl":"10.1016/j.talanta.2026.130180","url":null,"abstract":"<p><p>Epigenomic and epitranscriptomic alterations-particularly RNA methylations such as N⁶-methyladenosine (m6A), N⁵-methylcytosine (m5C), N¹-methyladenosine (m1A), and N⁷-methylguanosine (m7G)-are increasingly recognized as pivotal regulators in the pathobiology of colorectal cancer (CRC) and other highly prevalent disorders. Despite their growing clinical and biological relevance, rapid and robust electrochemical strategies enabling the single (m1A, m7G) or multiplexed quantification of these RNA modifications remain unavailable. Herein, we report an indirect competitive, four-analyte amperometric immunoassay that enables the simultaneous determination of m6A, m5C, m1A, and m7G. BSA-conjugated methylated synthetic nucleotides covalently attached to magnetic carboxylated microsized-beads (HOOC-MμBs) competed with freely methylated nucleotides in the target sample for specific anti-epimark detection antibodies. Thereafter, labeling with horseradish peroxidase (HRP)-conjugated secondary antibodies (Ab2-HRP) enabled amperometric readout at screen-printed carbon electrodes (SPCEs) for single or multiplexed transduction. The developed bioplatforms exhibited high selectivity, negligible cross-interference, and a significantly reduced assay time (30 min), supporting their integration into a robust multiplexed format. Application in CRC cellular scenarios, using only nanograms of total RNA, allowed differential profiling of basal and pathway-activated states across five cell lines, revealing distinct RNA methylation patterns associated with diverse biological and genetic backgrounds. Moreover, as proof of concept, an octuple-detection configuration was implemented for parallel analysis of the four methylations in two cell types, wild-type and harboring the clinically relevant KRAS G12V mutation, allowing the evaluation of associations between the target epimark expression levels and this oncogenic point mutation.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130180"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric swab and turbidimetric sensing for dual-mode point-of-use testing of demeton pesticide in foods based on oxidase-like Cu₂O@Ag nanoprobe.","authors":"Yi Liu, Yinxia Yang, Jialin Liang, Lei Han, Qian Zhao, Jie Luo, Shi Gang Liu","doi":"10.1016/j.talanta.2026.130192","DOIUrl":"10.1016/j.talanta.2026.130192","url":null,"abstract":"<p><p>Dual-mode sensing can enhance detection reliability by cross-validating results from distinct signals. Herein, a colorimetric and turbidimetric dual-mode sensing platform based on Cu₂O@Ag nanozyme with robust oxidase-like activity is developed for point-of-use (POU) detection of demeton. The Cu₂O@Ag efficiently catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), producing concurrent absorbance and turbidity signals. Demeton blocks active sites and suppresses catalytic activity in a concentration-dependent manner. Based on the principle, two modes for POU testing of demeton are developed. The first mode is based on a smartphone-assisted colorimetric swab and shows a linear range of 50 - 4000 ng/mL with the detection limit of 0.190 ng/mL. The other is based on a portable turbidimeter and exhibits a linear detection range of 50 to 3000 ng/mL with a detection limit of 2.997 ng/mL. The approaches are successfully validated in real food samples including baby cabbage, lettuce, cilantro, and tea leaves. This work presents a rapid, low-cost, and instrument-flexible dual-mode sensing strategy for on-site analysis of demeton residue. Also, the dual-mode detection based on colorimetric and turbidimetric signals for the first time offers a novel insight for dual-mode sensing.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130192"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}