Talanta最新文献

{"title":"Quantitative approaches for spatial metabolomics with isomer differentiation using surface sampling capillary electrophoresis mass spectrometry","authors":"Anastasia Golubova,&nbsp;Ingela Lanekoff","doi":"10.1016/j.talanta.2025.128482","DOIUrl":"10.1016/j.talanta.2025.128482","url":null,"abstract":"<div><div>The importance of metabolites and their isomeric structures in biological function and dysfunction is increasingly recognized. However, achieving quantitative mapping of metabolites within tissue regions, particularly with isomeric specificity, remains an analytical challenge. This work presents the development of a quantitative surface sampling capillary electrophoresis method for spatial metabolomics with isomeric resolution. Five quantitation strategies were evaluated, with the optimal approach identified as sequential injection of metabolites directly from tissue alongside standards. This methodology was applied to a rat brain tissue section in a proof-of-principle study, enabling quantitative spatial analysis of metabolites, neurotransmitters, and isomeric species. Among the findings, the aromatic amino acids tyrosine, phenylalanine, and tryptophan exhibited the most dynamic distributions across four brain regions, while leucine and isoleucine demonstrated distinct spatial profiles, with leucine consistently being the more abundant isomer. This method offers a promising tool for advancing the understanding of spatially resolved biochemical processes underlying biological function and dysfunction.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128482"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach for profiling of nature-inspired and bioactive proteins and peptides isolated from selected medicinal plants and their implications in Alzheimer diseases by in-silico analysis","authors":"Abinaya Kanagaraja ,&nbsp;Nagasathiya Krishnan ,&nbsp;Anantha Krishnan Dhanabalan ,&nbsp;Munusamy Thirumavalavan ,&nbsp;Velmurugan Devadasan ,&nbsp;Pachaiappan Raman","doi":"10.1016/j.talanta.2025.128481","DOIUrl":"10.1016/j.talanta.2025.128481","url":null,"abstract":"<div><div>This study reported the profiling and the <em>in-silico</em> analysis of the therapeutic potential of proteins/peptides (for Alzheimer disease) isolated from <em>Tinospora cordifolia, Evolvulus alsinoides, Centella asiatica</em> and <em>Convolvulus pluricaulis.</em> The proteins/peptides were extracted by using four different pH based buffer solutions. The trypsin digested proteins/peptides were analyzed by LC-MS/MS based peptide mass fingerprinting which showed the presence of high number of proteins/peptides involved in regulating the oxidative stress. The sequential purification with 10 kDa and 3 kDa cut-off ultrafiltration membranes for buffer based extracted proteins/peptides was performed. The evaluation of crude and these filtrates revealed the highest antioxidant potential for 3 kDa cut-off filtrate of 0.1 M Tris HCl buffer (pH 8.0) from FRAP, DPPH, ABTS and NOS assays. The presence of peptides in 3 kDa cut-off filtrates was detected by HPLC, identified by MALDI-TOF MS and the fragmentation pattern was obtained by LC-MS/MS. The <em>in-silico</em> docking study revealed that the identified peptides showed the highest binding affinity against the Alzheimer targets (BACE1, nAChR, Aβ, AChE, GSK-3β, JNK). Thus, the findings of this study provided the preliminary evidence for the antioxidant and neuroprotective potential of the selected medicinal plants, by supporting their relevance in delaying the onset of neurodegeneration and highlighting their prospects for drug development.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128481"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterials-mediated electrochemical detection of neutrophil gelatinase-associated lipocalin for chronic kidney disease: Advances, challenges, and future prospects","authors":"Poornima Bhatt ,&nbsp;Arslan Gour ,&nbsp;Deepak Kukkar ,&nbsp;Ki-Hyun Kim","doi":"10.1016/j.talanta.2025.128473","DOIUrl":"10.1016/j.talanta.2025.128473","url":null,"abstract":"<div><div>Chronic kidney disease (CKD) is characterized by a gradual and permanent deterioration in kidney function, resulting in disruption of the body's internal balance. Traditional diagnostic methods based on serum metabolites often suffer from inaccuracy for CKD diagnosis due to interference from factors such as age, gender, and comorbidities. Neutrophil gelatinase-associated lipocalin (NGAL) is considered an effective clinical marker for early and reliable CKD diagnosis due to its rapid elevation in both serum and urine following renal tubular injury. Among various diagnostic strategies, electrochemical (EC) sensing stands out for its high sensitivity, reproducibility, and suitability for complex biological matrices. The integration of nanomaterials (NMs) with EC platforms further enhances detection performance, enabling precise, real-time monitoring of NGAL levels. This review systematically assesses recent progress in EC-based NGAL biosensors, compares NGAL with other CKD biomarkers, and highlights key design parameters, current limitations, and future opportunities for clinical translation. It specifically presents the unique relevance of NGAL for early CKD diagnosis, followed by a brief discussion on the distinctive advantages of NMs in enhancing EC sensing potentials. Various types of EC biosensors developed based upon potentiometric, amperometric, and conductometric principles are then examined in detail. The review concludes with insights into the current challenges hindering NGAL sensor development and prospective strategies for advancing their clinical applicability.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128473"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lab-In-Syringe automation of dispersive micro-solid phase extraction based on in situ formation of Ni/Fe-layered double hydroxides for the determination of angiotensin receptor blockers in environmental waters","authors":"Hilal Rabia Çevik ,&nbsp;Tuğçe Özyiğit ,&nbsp;Arzu Kocaoğlu ,&nbsp;Burkhard Horstkotte ,&nbsp;Sercan Yıldırım","doi":"10.1016/j.talanta.2025.128489","DOIUrl":"10.1016/j.talanta.2025.128489","url":null,"abstract":"<div><div>Pharmaceutical residues in environmental waters continue to raise significant public and ecological concerns, necessitating advanced analytical methodologies for their monitoring. In this work, a novel Lab-In-Syringe automated dispersive micro solid-phase extraction (LIS-DMSPE) method was developed to determine three angiotensin receptor blockers in water samples. The approach was based on the <em>in situ</em> synthesis of Ni/Fe-layered double hydroxides within the void of an automatic syringe pump through controlled pH adjustment following the aspiration of precursor solutions. This enabled the rapid formation of the adsorbent and eliminated the need for pre-synthesized or magnetized sorbents. Enhanced sedimentation speed, achieved by increasing ionic strength with NaNO₃, allowed isolating the sedimented sorbent from the sample matrix without centrifugation and filtration. The instrumental setup was successfully coupled online with HPLC-DAD. After in-syringe washing and dissolving the sediment, the low organic solvent content of the extract enabled large-volume injection (200 μL), thereby boosting sensitivity. Parameters including type and volume of precursor solution, NaNO₃ addition, buffer volume and pH, stirring rate and time, and the composition of elution/destruction solution were carefully optimized. Recoveries and enrichment factors were in the ranges of 62.0–88.7 % and 13.3–25.8, respectively. The method was linear over a 5–200 μg L⁻¹ concentration range for all analytes. Accuracies ranged from 88.7 % to 105.8 % for real samples spiked at two concentrations with RSDs less than 3.5 %. To our knowledge, this is the first report on a LIS-DMSPE method that does not require pre-synthesis or magnetization of the sorbent for extraction and uses accelerated sedimentation for adsorbent isolation.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128489"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robust removal of thallium(I) from water with nano-MnO2 implanted zwitterionic porous hydrogel","authors":"Hongjie Wu ,&nbsp;Dirong Gong ,&nbsp;Xiaoyu Jia","doi":"10.1016/j.talanta.2025.128484","DOIUrl":"10.1016/j.talanta.2025.128484","url":null,"abstract":"<div><div>The uncontrolled disposal of Tl(I) waste comes at a significant economic and environmental cost because of the acute toxicity and high mobility. To resolve the aggregation problem for most reported MnO₂ based adsorbent materials which significantly affected the adsorption efficiency, porous zwitterionic ammonium propyl sulfonate (zw) polyacrylamide hydrogel (PAAm) has been fabricated to disperse nano-MnO₂ as a support. The developed nano-MnO₂@PAAm-zw exhibited a specific surface area of 5.06 m²/g and a pore diameter of 23.8 nm. particularly amenable to removal of Tl(I) for environmental remediation. The factors, including adsorbent MnO₂ incorporation, feeding amount, adsorption time, pH of the solution, Tl(I) initial concentration and existence of competing cations that governing the adsorption performances were fully elucidated. The maximal removal capacity <em>Q</em>_<em>e</em> reaches up to 336.5 mg (Tl) g⁻¹(MnO₂) with 99.92 % elimination efficiency and a low residue of 4.5 μg L⁻¹. The materials exhibit an astonishing robustness against nature matrix and high concentration of cations with adsorption capacity being 928.6–41.43 times larger than various competing counterparts for lab-made and mineral samples, representing the state-of-the-art level of adsorbents. The adsorbent can be regenerated at least five times retaining 86.0 % of original efficiency. A novel concerted mechanistic pathway of retention mainly associated with re-dox reaction is possible. These features offer great potentials for selective and deep elimination of total thallium in water samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128484"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of gunshot residue from nontoxic ammunition by laser-induced breakdown spectroscopy and machine learning algorithms.","authors":"Rodrigo Wenceslau, Jader S Cabral, Gabriel da Silva Souza, Felipe Lopes Rodrigues Silva, Giorgio S Senesi, Edenir Rodrigues Pereira-Filho, Cicero Cena, Matheus Cicero Ribeiro, Bruno S Marangoni","doi":"10.1016/j.talanta.2025.128483","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128483","url":null,"abstract":"<p><p>Gunshot residue (GSR) is defined as particles generated upon the discharge of ammunition from a firearm. The main components of ammunition include the primer, cartridge case, and bullet. GSR particles originated from a combination of these components as well as from internal firearm parts. For conventional ammunition, GSR can be reliably identified by detecting Pb, Ba, and Sb using scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS). In contrast, GSR from nontoxic ammunition lacks these markers, making SEM-EDS detection ineffective. Laser-induced breakdown spectroscopy (LIBS) was used to analyze GSR-NTA particles collected directly from shooters' hands to identify potential chemical fingerprints. Spectra were acquired across two spectral ranges (186-1042 nm and 186-570 nm), and elements such as H, N, O, C, Ti, Zn, Cu, Ba, Sr, Fe, Mg, and Al were detected. Multivariate analysis and machine learning (ML) algorithms were applied. The dataset was divided into training and external validation sets, with linear discriminant analysis (LDA) achieving 100 % classification accuracy. Spectral analysis revealed that Zn, Ti, Cu, and Fe were the primary elements responsible for sample differentiation, with minor contributions from Ba and Sr. In conclusion, the combination of LIBS and ML shows potential as a forensic tool for identifying GSR-NTA particles on the hands of individuals who have, or have not, discharged a firearm.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128483"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges of Carbon Dots Immobilization – A review","authors":"Marita A. Cardoso ,&nbsp;Paula Martins-Lopes ,&nbsp;Helena M.R. Gonçalves","doi":"10.1016/j.talanta.2025.128477","DOIUrl":"10.1016/j.talanta.2025.128477","url":null,"abstract":"<div><div>Given the potential of Carbon Dots and their wide applications, strategies for their immobilization are vital for the development of specific, stable and sensitive (bio)chemical analysis. In this review we start by demonstrating the most common and efficient immobilization procedures used in Cdots. Subsequently, we describe the Carbon dots-host nanocomposites synthesis, properties, and ultimately outline and revise the fields where they have been used by presenting applications in this field. The Carbon dots-host nanocomposites enhanced properties are also explored, namely: increase photoluminescence intensity and electron transfer rates, induce light absorption, bandgap tuning, abundant introduction of functional groups, lower toxicity and biocompatibility.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128477"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-activated fluorescent NO donor enables spatiotemporal tracking and stomatal closure in Arabidopsis thaliana","authors":"Huan Liu ,&nbsp;Xueting Wang ,&nbsp;Xinyi Cai ,&nbsp;Hailiang Zhu ,&nbsp;Qingcai Jiao ,&nbsp;Xueao Wang ,&nbsp;Yong Qian","doi":"10.1016/j.talanta.2025.128460","DOIUrl":"10.1016/j.talanta.2025.128460","url":null,"abstract":"<div><div>We designed P1G3, a light-triggered NO donor from N-nitroso-naphthalene derivatives, releasing NO via N–NO cleavage while producing fluorescent P1G2 for real-time tracking. The quantity of NO released from P1G3 in the cytoplasm can be precisely regulated by light duration and light intensity, thereby minimizing the risk of unintended self-leakage in biological settings. Utilizing a quantitative mapping relationship between nitric oxide release and fluorescence intensity, we calculated for the first time the nitric oxide conversion rate of a donor system in a cellular environment. Both a method for nitric oxide quantification is proposed and the stable conversion efficiency of this donor is demonstrated. In <em>Arabidopsis thaliana</em>, P1G3 successfully induced Ca²⁺ accumulation in guard cells and triggered the gradual closure of stomata, demonstrating spatiotemporal-controlled NO delivery for enhanced drought tolerance.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128460"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photo-potentiometry: Sensing of sugars using a pH-probe coated with a film of intrinsically microporous polyamine containing graphitic carbon nitride photocatalyst","authors":"Fernanda C.O.L. Martins ,&nbsp;Wanessa R. Melchert ,&nbsp;Mariolino Carta ,&nbsp;Neil B. McKeown ,&nbsp;Frank Marken","doi":"10.1016/j.talanta.2025.128461","DOIUrl":"10.1016/j.talanta.2025.128461","url":null,"abstract":"<div><div>At proof-of-concept level, the photochemical transformation of glucose (or more generally of carbohydrates) can be detected analytically as a localised pH change. Using a conventional potentiometric pH-probe, a microporous coating is developed to explore carbohydrate sensing in the 200–800 μM concentration range based on localised pH changes induced by light. The photo-responsive film is based on fibrous cellulose (to aid permeability), photocatalytic graphitic carbon nitride (g-C₃N₄), and an intrinsically microporous polyamine host (PIM-EA-TB, as reaction environment and binder). The film-modified pH-probe is pre-conditioned in a pH 4 buffer (containing phthalate buffer). When immersed in an aqueous solution, switching on a blue LED (λ = 385 nm, approx. 60 mW cm⁻²) causes a pH transient towards alkaline, which is correlated with the carbohydrate concentration (all three glucose, fructose, or sucrose give very similar signals). The LOD is typically 70 μmol dm⁻³, with a linear range up to 800 μmol dm⁻³. Non-linearity beyond 800 μmol dm⁻³ is tentatively attributed to limited oxygen availability. The photo-electroanalytical mechanism is discussed in terms of competing proton generation and consumption in the photoactive film linked to oxygen depletion (causing alkaline drift) at the pH-probe surface.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128461"},"PeriodicalIF":5.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ergonomic and fully 3D–printed, activated, and modified electrochemical set–up for electroanalytical paracetamol detection","authors":"Grzegorz Kowalski ,&nbsp;Karolina Kwaczyński ,&nbsp;Lukasz Poltorak","doi":"10.1016/j.talanta.2025.128467","DOIUrl":"10.1016/j.talanta.2025.128467","url":null,"abstract":"<div><div>In this work, we manufactured the entire electrochemical configuration (all electrodes along with the ergonomic cell), which was 3D<strong>–</strong>printed using fused deposition modelling (FDM) technology. All electrodes were printed from carbon black/poly(lactic acid) (CB/PLA) filament, while the cell body was produced with neutral PLA. An activation process was conducted to enhance the electrochemical properties of all electrodes (working electrode (WE), counter electrode (CE), and reference electrode (RE)), which included immersion in dichloromethane followed by anodic, and cathodic treatment. The WE was only subjected to the activation process, providing the best output when the synergistic action of organic solvent and electrochemical electrode surface treatment were used. The novel aspects of this work originate from the applied electrode surface treatment, adjustment of all three electrodes' placement in an ergonomic and fully 3D printed cell, and finally, RE and CE properties adjustment. For the latter, the REs properties were defined by electrodeposition of silver particles further covered with AgCl, providing a stable and constant reference potential. The CE was made out of 3D–printed CB/PLA modified with platinum particles, which enhanced its electric conductivity. Even though, in both cases, the surface coverage was found to be nonhomogeneous the electrodes displayed desired properties, creating cheap substitutions to commercially available components. All electrodes were comprehensively inspected with a range of characterization techniques, including voltammetry, chronoamperometry, electrochemical impedance spectroscopy, scanning electron microscopy, optical profilometry, attenuated total reflectance Fourier–transform infrared spectroscopy, laser–induced breakdown spectroscopy along with surface wettability studies. The electrochemical behavior of the system, in the presence of a model redox probe, ferrocenemethanol, was analyzed using cyclic voltammetry. Finally, our fully 3D<strong>–</strong>printed sensing platform was assessed for paracetamol determination giving the limit of detection and limit of quantification to be 0.38 μM and 1.26 μM, respectively. The system was successfully applied to the determination of paracetamol in a pharmaceutical tablet using the standard addition method, confirming its suitability for quantitative analysis in complex sample matrices.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"Article 128467"},"PeriodicalIF":5.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}