Talanta最新文献_第10页

A novel solid-phase extraction method for separation and preconcentration of Nickel(II) in hydrogenated vegetable oils using Sudan III functionalized Fe3O4 采用苏丹丹III功能化Fe3O4固相萃取分离富集氢化植物油中镍（II）

IF 5.6 1区化学

Talanta Pub Date : 2025-04-15 DOI: 10.1016/j.talanta.2025.128164

Enes Alegöz , Sema Bağdat , Feyzullah Tokay

{"title":"A novel solid-phase extraction method for separation and preconcentration of Nickel(II) in hydrogenated vegetable oils using Sudan III functionalized Fe3O4","authors":"Enes Alegöz , Sema Bağdat , Feyzullah Tokay","doi":"10.1016/j.talanta.2025.128164","DOIUrl":"10.1016/j.talanta.2025.128164","url":null,"abstract":"<div><div>This study presents a novel approach for separation/preconcentration of Ni(II) from hydrogenated vegetable oils prior to FAAS detection. Sudan III modified Fe<sub>3</sub>O<sub>4</sub> that was characterized by FTIR and SEM, was utilized as solid phase extractor. Critical parameters effective on sorption and elution of Ni(II) were optimized using central composite design. The optimum conditions were 58.3 s vortexing time, 0.22 g sorbent mass and 19.1 mL sample volume for sorption and 6 mL of 0.86 mol L<sup>−1</sup> HNO<sub>3</sub> with 95 s vortexing time for elution. LOD and LOQ of the method were 35.9 μg kg<sup>−1</sup> and 119.6 μg kg<sup>−1</sup>, respectively. Preconcentration factor was obtained as 3.2 and sample frequency was 23.4 h<sup>−1</sup>. Organometallic Ni standard was used in validation and recovery was 97 ± 4 % with 4.1 % precision. Applicability was proved by the analysis of commercial hydrogenated vegetable oils with recoveries between 93.0 and 108.6 %. Environmental sustainability was assessed using the Analytical GREENness calculator, resulting in a greenness score of 0.51. This method offers a sensitive, efficient, and eco-friendly approach for trace Ni(II) analysis in complex oily matrixes.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128164"},"PeriodicalIF":5.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-switchable dsDNA promoter regulates the activity of CRISPR-Cas12a for APE1 detection 结构可切换的dsDNA启动子调节CRISPR-Cas12a的活性以检测APE1

IF 5.6 1区化学

Talanta Pub Date : 2025-04-15 DOI: 10.1016/j.talanta.2025.128161

Xingrong Li , Yumei Li , Cuixiang Wang , Jiatong Chai , Hongmao Liu , Xinli Jiang , Yirong Li

{"title":"Structure-switchable dsDNA promoter regulates the activity of CRISPR-Cas12a for APE1 detection","authors":"Xingrong Li , Yumei Li , Cuixiang Wang , Jiatong Chai , Hongmao Liu , Xinli Jiang , Yirong Li","doi":"10.1016/j.talanta.2025.128161","DOIUrl":"10.1016/j.talanta.2025.128161","url":null,"abstract":"<div><div>Apurinic/apyrimidinic endonuclease 1 (APE1) has been considered as a promising biomarker since it is associated with numerous human diseases, involving neurodegenerative diseases and cancer. However, current APE1 detection methods mainly rely on immunology-based methods, which are burdened by time-consuming and procedural complexity. To overcome these shortcomings, we have developed an innovative all-in-one technique that simplifies APE1 detection by integrating enzyme-responsive elements structure-switchable dsDNA promoter with CRISPR/AsCas12a methodology, namely EDC. In this work, the structure-switchable dsDNA promoter has been well-designed to trigger the site-directed incision of APE1 and then release the split activator to illumine the CRISPR/AsCas12a catalyst system by coupling it with another truncated activator. Under optimal circumstances, the proposed strategy enables sensitive detection of the target APE1 with a detection limit of 4.8 × 10<sup>−5</sup> U/mL and a wide linear range from 5.0 × 10<sup>−5</sup> to 1.0 × 10<sup>−1</sup> U/mL. Moreover, this strategy could be gratifyingly applied to screen APE1 inhibitors and monitor APE1 in lysates from cell extractions or clinical serum samples. Overall, this study presents a novel approach that utilizes dsDNA promoter as programmable switching components, effectively enhancing CRISPR/Cas12a-based diagnostic platforms and demonstrating the significant potential for clinical translation.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128161"},"PeriodicalIF":5.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free electrochemiluminescence aptasensor for rapid and accurate detection of cardiac troponin I 用于快速准确检测心肌肌钙蛋白I的无标记电化学发光感应传感器

IF 5.6 1区化学

Talanta Pub Date : 2025-04-15 DOI: 10.1016/j.talanta.2025.128160

Xilin Chen , Yanli Li , Ling Chen , Junrong Tu , Jianbin Pan , Xiaoru Zeng , Yu Fu , Duanping Sun

{"title":"Label-free electrochemiluminescence aptasensor for rapid and accurate detection of cardiac troponin I","authors":"Xilin Chen , Yanli Li , Ling Chen , Junrong Tu , Jianbin Pan , Xiaoru Zeng , Yu Fu , Duanping Sun","doi":"10.1016/j.talanta.2025.128160","DOIUrl":"10.1016/j.talanta.2025.128160","url":null,"abstract":"<div><div>Acute myocardial infarction (AMI) is the most common cause of death in individuals with cardiovascular disease. Cardiac troponin I (cTnI) is acknowledged as the most prominent biomarker for AMI. However, the key problem of practical applications is how to effectively improve the detection speed and sensitivity. In this study, we designed a novel electrochemiluminescence (ECL) aptasensor for the rapid and quantitative determination of cTnI. This sensor employs self-luminous europium-based metal-organic framework@CdS quantum dots (Eu-MOF@CdS QDs) as a signal probe. The Eu-MOF@CdS QDs can produce a robust cathodic ECL signal via a synergistic effect. Furthermore, a ferrocene-labeled aptamer was used as a quenching probe for quenching the ECL emission of the Eu-MOF@CdS QDs. In the presence of cTnI, the ECL intensity increased with increasing cTnI concentration after ferrocene-labeled aptamer specifically recognized cTnI and was detached from the electrode interface. Under optimal conditions, the aptasensor demonstrated precise analytical capabilities for cTnI ranging from 1.0 <em>pg/mL</em> to 1.0 <em>ng/mL</em> with a notably low detection limit of 0.08 <em>pg/mL</em> within 60 min. The results show that the developed ECL sensing device demonstrates the potential applications and perspectives for the detection of cTnI in serum samples as well as in the field of biomedical analyses.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128160"},"PeriodicalIF":5.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in nanoparticle-enhanced paper sensor for detecting toxic metals in water 纳米粒子增强纸张传感器检测水中有毒金属的研究进展

IF 5.6 1区化学

Talanta Pub Date : 2025-04-14 DOI: 10.1016/j.talanta.2025.128146

Marzieh Sadat Hosseini , Rajiv Padhye , Xin Wang , Shadi Houshyar

{"title":"Advances in nanoparticle-enhanced paper sensor for detecting toxic metals in water","authors":"Marzieh Sadat Hosseini , Rajiv Padhye , Xin Wang , Shadi Houshyar","doi":"10.1016/j.talanta.2025.128146","DOIUrl":"10.1016/j.talanta.2025.128146","url":null,"abstract":"<div><div>Toxic metals in water pose a serious threat to public health and the environment, especially in regions with limited access to advanced laboratory infrastructure. Traditional methods for detecting toxic metals, such as atomic absorption spectrometry (AAS) and inductively coupled plasma mass spectrometry (ICP-MS), are accurate but expensive, complex, and unsuitable for on-site use. In contrast, paper-based analytical devices (PADs) offer a low-cost, and user-friendly alternative, especially in resource-limited areas. Recent advancements have significantly improved PAD performance, especially through the integration of nanoparticles and the use of enhanced colorimetric and electrochemical detection methods. These improvements have enabled faster, more sensitive detection while maintaining simplicity and field readiness. This review explores key advancements in PAD technology from 2015 to 2025 including advances in sensitivity, nanoparticle functionalization, and smartphone-based readouts. Unlike previous reviews, this study presents a comparative analysis of PAD detection mechanisms, evaluates commercialization and regulatory challenges, and explores emerging trends such as smartphone integration and microextraction techniques. By addressing these aspects, this review highlights key advancements and optimization strategies to enhance the stability, selectivity, and practical implementation of PADs for water quality monitoring.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128146"},"PeriodicalIF":5.6,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical immunoplatform for the quantification of epithelial extracellular vesicles applied to prostate cancer diagnosis 用于前列腺癌诊断的上皮细胞外囊泡定量的电化学免疫平台

IF 5.6 1区化学

Talanta Pub Date : 2025-04-13 DOI: 10.1016/j.talanta.2025.128130

Emiliano Felici , Coral González-Martínez , Teresa Valero Griñán , Sheila Gato-Zambrano , Sirley V. Pereira , Martín A. Fernández-Baldo , Francisco G. Ortega-Sanchez

{"title":"Electrochemical immunoplatform for the quantification of epithelial extracellular vesicles applied to prostate cancer diagnosis","authors":"Emiliano Felici , Coral González-Martínez , Teresa Valero Griñán , Sheila Gato-Zambrano , Sirley V. Pereira , Martín A. Fernández-Baldo , Francisco G. Ortega-Sanchez","doi":"10.1016/j.talanta.2025.128130","DOIUrl":"10.1016/j.talanta.2025.128130","url":null,"abstract":"<div><div>Prostate cancer (PCa) is the second most commonly diagnosed cancer in men worldwide, and its early detection is critical for improving patient outcomes through timely and effective treatment. In this work, we present the first electrochemical immunoplatform based on magnetic microbeads (MBs) for the determination of epithelial extracellular vesicles (EpEVs), which are emerging as promising biomarkers for PCa diagnosis and prognosis. The immunoplatform employs MBs functionalized with anti-EpCAM antibodies to selectively capture EpEVs, forming sandwich-type immune complexes that are detected via amperometry at disposable screen-printed carbon electrodes. The method demonstrated a detection limit of 0.4 ng μL<sup>−1</sup> of EpEVs obtained from PC-3 cell line's culture, excellent reproducibility (coefficient of variation <5 %), and high selectivity against potential interferences. Comparative analysis with colorimetric immune-magnet ELISA test showed a strong correlation between the two methods, confirming the reliability of the proposed approach. Furthermore, the electrochemical platform provided better precision and a lower limit of detection than the immune magnet ELISA method, indicating its superior analytical performance. Clinical validation using patient samples revealed that the combination of EpEV detection with PSA levels significantly improves the sensitivity and specificity of PCa diagnosis. This novel immunoplatform represents a promising analytical tool for early detection and monitoring of PCa, with potential applications in personalized cancer management.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128130"},"PeriodicalIF":5.6,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waveguide microwave gas sensor based on monolithic In2O3 for enhanced ammonia detection 基于单片In2O3的波导微波气体传感器增强氨检测

IF 5.6 1区化学

Talanta Pub Date : 2025-04-13 DOI: 10.1016/j.talanta.2025.128122

Ling Gao , Juhua Xu , Xianwang Yang , Chang Xu , Xiaolong Wang , Quan Jin

{"title":"Waveguide microwave gas sensor based on monolithic In2O3 for enhanced ammonia detection","authors":"Ling Gao , Juhua Xu , Xianwang Yang , Chang Xu , Xiaolong Wang , Quan Jin","doi":"10.1016/j.talanta.2025.128122","DOIUrl":"10.1016/j.talanta.2025.128122","url":null,"abstract":"<div><div>For commonly used chemical resistance sensors, achieving room-temperature detection often results in reduced performance. Microwave gas sensors have been rapidly expanding in recent years, attributed to their advantages of room-temperature operation and low power consumption. Waveguide, as a three-dimensional microwave circuit with separated electric and magnetic field distributions, resulting in high quality factor, is expected to significantly enhance the sensitivity of microwave sensors. Additionally, the hierarchical porous structure of monolithic In<sub>2</sub>O<sub>3</sub> facilitates gas diffusion, further enhancing sensing performance. Herein, a microwave gas sensor is designed by applying porous monolithic In<sub>2</sub>O<sub>3</sub> and waveguide resonator. By adjusting the content of the polymer PVP, In<sub>2</sub>O<sub>3</sub> with different pore sizes is synthesized in which hierarchical porous In<sub>2</sub>O<sub>3</sub> sample with a PVP content of 3.0 g (3.0-HPS In<sub>2</sub>O<sub>3</sub>) exhibits the best NH<sub>3</sub> sensing properties, including a wide concentration range (0.01–2000 ppm), a low limit of detection (LOD) of 7 ppb, excellent selectivity, good moisture resistance, and long-term stability. This study offers a promising approach for the development of room-temperature ammonia gas sensors with an extended detection range and has preliminarily explored the influence of pore sizes on gas sensing performance.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128122"},"PeriodicalIF":5.6,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantum dots encoded superparamagnetic micron-particles based on layer-by-layer assembly for multiplexed nucleic acid detection 基于逐层组装的量子点编码超顺磁性微米粒子，用于多路核酸检测

IF 5.6 1区化学

Talanta Pub Date : 2025-04-13 DOI: 10.1016/j.talanta.2025.128109

Xiaoshuang Zhao , Jieying Zhang , Yifan Chen , Ziting Chen , Zhe Liu , Yi Xu , Xingjun Zhu , Yang Li , Xianqiang Mi

{"title":"Quantum dots encoded superparamagnetic micron-particles based on layer-by-layer assembly for multiplexed nucleic acid detection","authors":"Xiaoshuang Zhao , Jieying Zhang , Yifan Chen , Ziting Chen , Zhe Liu , Yi Xu , Xingjun Zhu , Yang Li , Xianqiang Mi","doi":"10.1016/j.talanta.2025.128109","DOIUrl":"10.1016/j.talanta.2025.128109","url":null,"abstract":"<div><div>With the rapid development of precise medicine, microbeads-based fluorescence bio-coding technology for multiplexed detection has attracted increasing attentions. However, traditional multiplexed bio-analysis could not meet the demand for multiplexed detection due to the limitations of labeling dyes and microscope channels. Here, a novel fluorescence encoding strategy was proposed by using multi-color quantum dots (QDs) acted as fluorescence tags and micron sized superparamagnetic particle acted as encoding carrier. By adjusting the color and number of labeled QDs, fluorescence encoded superparamagnetic particles (FESPs) with distinct fluorescence colors and intensities were constructed. The FESPs were prepared by metal coordination-based layer-by-layer assembly with excellent binding effect. By binding DNA capture oligonucleotides, FESP probes were constructed for multiplexed detection of miRNA-21, miRNA-122 and miRNA-130b with excellent specificity. Moreover, the miRNA targets could be easily separated within 5 s originated from the excellent superparamagnetism, enabling great simplified bio-analysis process. The results demonstrated here will provide opportunities to realize the full potentials of fluorescence bio-coding in biomedical detection and diagnosis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128109"},"PeriodicalIF":5.6,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of universal isotachophoresis – capillary zone electrophoresis method for trace determination of bromide in complex ionogenic samples 开发用于痕量测定复杂离子源样品中溴化物的通用等位电泳-毛细管区带电泳方法

IF 5.6 1区化学

Talanta Pub Date : 2025-04-11 DOI: 10.1016/j.talanta.2025.128113

Marián Masár, Jasna Hradski, Roman Szucs

{"title":"Development of universal isotachophoresis – capillary zone electrophoresis method for trace determination of bromide in complex ionogenic samples","authors":"Marián Masár, Jasna Hradski, Roman Szucs","doi":"10.1016/j.talanta.2025.128113","DOIUrl":"10.1016/j.talanta.2025.128113","url":null,"abstract":"<div><div>Trace determination of bromide in water samples and high-purity chemicals was accomplished by capillary zone electrophoresis (CZE) with online isotachophoresis (ITP) sample pretreatment and photometric detection at 200 nm. The adverse effect of bromide on human health necessitates its monitoring in drinking water. Bromide is also a precursor of bromate, a carcinogenic by-product of water disinfection that can be formed in drinking water during ozonation (oxidation of bromide to bromate). ITP-CZE separations were performed at acidic pH (2.7) as this facilitates high selectivity of the separation system. Addition of the zwitterionic surfactant <em>N</em>-dodecyl-<em>N</em>,<em>N</em>-dimethyl-3-ammonio-1-propanesulfonate to the leading electrolyte enhanced the separation of bromide from anionic macrocomponents in drinking water. The first separation step, ITP, served as a very efficient sample clean-up. Linear response was observed in the presence and absence of matrix in the concentration range 1–50 μg/L. ITP-CZE separations were characterized by very good repeatability of migration times (up to 0.4 % RSD) as well as peak areas (up to 3.7 % RSD) of bromide. The achieved limit of detection for bromide was 0.3 μg/L in the presence of matrix. To assess the practical applicability of the developed test procedure, samples of mineral and drinking water, as well as highly pure chloride salts, were analyzed. Recoveries of bromide in the analyzed samples were in the range of 98–103 %. The developed ITP-CZE method can be used for highly selective determination of trace concentrations of bromide present in ionogenic samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128113"},"PeriodicalIF":5.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To take unseen: direct gel-supported liquid extraction for multi-technique characterization of organic colourants in paint layers 未曾谋面：直接凝胶支撑液体萃取用于油漆层中有机着色剂的多技术表征

IF 5.6 1区化学

Talanta Pub Date : 2025-04-11 DOI: 10.1016/j.talanta.2025.128041

Adele Bosi , Alessandro Ciccola , Ilaria Serafini , Sanne Berbers , Art Néss Proano Gaibor , Elke Cwiertnia , Paolo Postorino , Gabriele Favero , Maarten R. van Bommel

{"title":"To take unseen: direct gel-supported liquid extraction for multi-technique characterization of organic colourants in paint layers","authors":"Adele Bosi , Alessandro Ciccola , Ilaria Serafini , Sanne Berbers , Art Néss Proano Gaibor , Elke Cwiertnia , Paolo Postorino , Gabriele Favero , Maarten R. van Bommel","doi":"10.1016/j.talanta.2025.128041","DOIUrl":"10.1016/j.talanta.2025.128041","url":null,"abstract":"<div><div>The study of organic colourants employed in artworks is a fundamental topic for history and conservation research; however, the most critical aspect in such matter arises from the necessity of sampling. Instead of binding media and inorganic components, natural organic colourants often exhibit weak signals during non-invasive analyses, which can be easily masked in complex mixtures, such as paint layers. To overcome this problem, the preferred approach is to take a sample, extract the colourants and analyse them using specific analytical techniques. The proposed method of direct agar gel-supported liquid extraction, performed using a Pasteur pipette tip, offers a promising solution to this challenge. By extracting colourants without the need for paint sample removal, it minimises visible impact on the artwork while still providing sufficient material for spectroscopic and mass spectrometric characterization. The experimental approach, which involved mock-up of mixing different organic and inorganic pigments with different binders and subjecting them to artificial ageing, simulates real-world complex matrices and assesses the effectiveness of the extraction method. Photomicrographs were used to document the procedure's visible impact. Mass spectrometry and surface-enhanced Raman scattering spectroscopy, the techniques of choice for invasive analysis of organic colourants, were used on the analytes extracted from the gel. The method application on paint patches from historical catalogues, housed in the <em>Stiftung Preußischer Kulturbesitz</em> collection, adds practical relevance to the study and demonstrates the applicability of the technique to authentic artworks. The procedure enabled the identification of natural and synthetic colourants without compromising the integrity of the paint systems.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128041"},"PeriodicalIF":5.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of metabolic pathways of racemic ketamine and its (S)-enantiomer in rat blood plasma using CE-ESI/MS with partial filling of dual chiral selector system 利用部分填充双手性选择器系统的 CE-ESI/MS 研究外消旋氯胺酮及其 (S) 对映异构体在大鼠血浆中的代谢途径

IF 5.6 1区化学

Talanta Pub Date : 2025-04-11 DOI: 10.1016/j.talanta.2025.128129

Renáta Konášová , Dušan Koval , Petr Tůma , Šimon Vaculín , Václav Kašička

{"title":"Study of metabolic pathways of racemic ketamine and its (S)-enantiomer in rat blood plasma using CE-ESI/MS with partial filling of dual chiral selector system","authors":"Renáta Konášová , Dušan Koval , Petr Tůma , Šimon Vaculín , Václav Kašička","doi":"10.1016/j.talanta.2025.128129","DOIUrl":"10.1016/j.talanta.2025.128129","url":null,"abstract":"<div><div>Ketamine is a chiral drug used as anesthetic, analgesic and antidepressant. Its enantiomers and stereoisomers of its metabolites show different pharmacological and behavioral effects. To study the ketamine metabolic pathway and investigate these effects, highly sensitive and enantioselective methods are required. For that reason, in this study, a new CE method using a partial filling dual chiral selector system and ESI-MS detection has been developed and applied for separation and quantification of enantiomers of ketamine and its main metabolites, norketamine, hydroxynorketamine and dehydronorketamine, extracted by dichloromethane from the blood plasma of laboratory rats. The dual chiral selector system consisting of two zones of highly sulfated β-cyclodextrin (30 mg mL<sup>−1</sup>) and highly sulfated γ-cyclodextrin (10 mg mL<sup>−1</sup>) was introduced consecutively near the capillary outlet end. Both chiral selectors were dissolved in the background electrolyte composed of 10 mM ammonium hydroxide, 104 mM acetic acid, 10 % (v/v) ethanol, pH∗ 3.75. This system enabled enantioseparation of ketamine and its metabolites within a single CE run. High resolutions (3.99–17.61) of enantiomers of all above four analytes within a short time (11 min) were achieved in the fused silica capillary covalently coated with weakly negatively charged polyanionic copolymer (poly(acrylamide-<em>co</em>-sodium-2-acrylamido-2-methylpropanesulfonate), PAMAMPS). This coating minimized analyte sorption to the capillary and provided good repeatability of migration times. The limits of detection and quantification of the above analytes were in the range 108–238 nM and 361–792 nM, respectively. The method was linear within wide concentration range of 0.1–200 μM and the recovery was 91.3–105 %.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128129"},"PeriodicalIF":5.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0