Talanta最新文献

{"title":"The application of desorption electrospray ionization mass spectrometry and mass spectrometry imaging in metabolomics, lipidomics and proteomics analysis","authors":"Haofan Liu ,&nbsp;Sicheng Huang ,&nbsp;Lina Yang ,&nbsp;Yaqian He ,&nbsp;Yongshuai Jing ,&nbsp;Yinghua Xie ,&nbsp;Beibei Hu ,&nbsp;Zhongqiu Li ,&nbsp;Haichao Bi ,&nbsp;Zhiwei Li","doi":"10.1016/j.talanta.2025.128611","DOIUrl":"10.1016/j.talanta.2025.128611","url":null,"abstract":"<div><div>At present, mass spectrometry (MS) and mass spectrometry imaging (MSI) have developed into versatile analytical techniques, applicable for the analysis of both small molecules and macromolecular compounds and complexes. Desorption electrospray ionization (DESI), as an ambient MS (AMS) technique, enables rapid analysis and imaging under open conditions with minimal or no sample preparation required. In this review, the principles and mechanisms of DESI were described, especially for the factors affecting the analysis including suppression effects, device condition parameter settings, and ionization and transmission efficiency. Moreover, the relevant improvements of DESI technique were introduced in detail. Emphasis has been made to discuss the recent research on the application of DESI MS and MSI in the fields of metabolomics, lipidomics, and proteomics. In these applications, DESI MS and MSI exhibit exceptional capabilities for qualitative analysis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128611"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic chip coupled with photoelectrochemical/fluorescence dual-modal sensing system for the efficient enrichment and detection of circulating tumor cells","authors":"Qingfeng Lin ,&nbsp;Xiaoyang Chen ,&nbsp;Yawen Hong ,&nbsp;Jingyu Huang ,&nbsp;Jiuhong Li ,&nbsp;Yanying Wang ,&nbsp;Lei Chen ,&nbsp;Chunya Li ,&nbsp;Gang Luo","doi":"10.1016/j.talanta.2025.128613","DOIUrl":"10.1016/j.talanta.2025.128613","url":null,"abstract":"<div><div>Conventional percutaneous lung biopsy and imaging modalities are often associated with adverse effects and may yield false-negative results, limiting their clinical efficacy. Circulating tumor cells (CTCs) are pivotal mediators in early tumorigenesis and metastatic dissemination. The detection of CTCs offers unparalleled advantages for early diagnosis and metastasis monitoring. NCI–H460 and NCI–H1650 cell lines serve as representative models for non-small cell lung cancer (NSCLC) and are critical biomarkers given that NSCLC accounts for over 85 % of lung cancer cases. Effective enrichment and precise identification of CTCs from whole blood are essential for early diagnostic applications. Herein, a microfluidic platform integrated with a dual-modal detection system, comprising photoelectrochemical (PEC) sensing and fluorescence imaging, was fabricated for the enrichment and quantification of CTCs (NCI–H460 and NCI–H1650 cells). The Hcy-Thiol probe-labeled CTCs in whole blood were separated by a microfluidic chip, and then a cathodic photoelectrochemical sensing system based on different aptamer-modified CuInS₂ nanoflowers was used to selectively capture CTCs and provide photocurrent signals, thus achieving fluorescence/photoelectrochemical dual-mode detection. Under the optimized conditions, the separation efficiency and purity of CTCs were 83.4 % and 76.8 %, respectively. Quantitative analysis of spiked blood samples at 100 cells/mL yielded cell counts of 107 ± 5 NCI–H460 cells and 87 ± 4 NCI–H1650 cells via photocurrent responses, corroborated by fluorescence signals with counts of 110 ± 4 and 85 ± 3 cells/mL, respectively. This microfluidic integrated with PEC/fluorescence dual-modal sensing system exhibits promising potential for early NSCLC detection and metastasis evaluation with high specificity and sensitivity.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128613"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an SI-traceable magnetic solid-phase extraction coupled with isotope dilution LC-MS/MS method for accurate quantification of alpha-fetoprotein in human serum","authors":"Haofeng Sun ,&nbsp;Jianyi Liu ,&nbsp;Qi Zhang ,&nbsp;Lei Yang ,&nbsp;Dan Song ,&nbsp;Min Zhou ,&nbsp;Dewei Song","doi":"10.1016/j.talanta.2025.128606","DOIUrl":"10.1016/j.talanta.2025.128606","url":null,"abstract":"<div><div>Alpha-fetoprotein (AFP) is a critical biomarker widely used for the screening and diagnosis of hepatocellular carcinoma and germ cell tumors. Although immunoassays are commonly employed for AFP detection, significant discrepancies in measurement results across different laboratories persist due to poor harmonization among methods. To improve the accuracy and harmonization of AFP quantification, this study developed a novel magnetic solid-phase extraction coupled with isotope dilution liquid chromatography-tandem mass spectrometry (MSPE-ID-LC-MS/MS) method for the precise measurement of AFP in serum. The method utilizes high-affinity AFP antibodies cocktails conjugated with magnetic nanoparticles to enhance the recovery of low-abundance AFP, combined with optimized elution conditions to ensure analytical reliability. The protocol involves one-step denaturation and alkylation, followed by tryptic digestion. This enables accurate quantification with SI-traceability using three signature peptides. Method validation demonstrated that intra- and inter-day precisions were &lt;10 %, recovery rates of 99.8–101.4 %, and the limit of quantification (LOQ) of 1.5 ng/mL. This work establishes a metrologically robust reference procedure for AFP measurement and paves the way for standardization of clinical protein assays.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128606"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative lipidomics for three-dimensional cell culture using deuterium oxide metabolic labeling","authors":"Jonghyun Kim ,&nbsp;Kyoung-Jin Choi ,&nbsp;Sung Bum Park ,&nbsp;Yoon-Ju Na ,&nbsp;Ki Young Kim ,&nbsp;Tae-Young Kim","doi":"10.1016/j.talanta.2025.128612","DOIUrl":"10.1016/j.talanta.2025.128612","url":null,"abstract":"<div><div>Three-dimensional (3D) cell culture offers a more physiologically relevant model than traditional two-dimensional culture, yet standardized methods for lipid quantification in 3D systems are lacking. This study presents a novel quantitative lipidomic approach combining 3D culture with deuterium oxide (D₂O) metabolic labeling to provide comprehensive insights into metabolic alterations. Using a hydrogel-based 3D system, we cultured preadipocytes and adipocytes, incorporating macrophage co-culture to induce insulin resistance. Relative lipid quantification was achieved using D₂O labeling for global omics relative quantification (DOLGOReQ). This method enabled the quantification of hundreds of lipids across major categories, including glycerolipids, glycerophospholipids, fatty acyls, and sphingolipids, while also revealing cell-type-specific D-labeling efficiencies. DOLGOReQ analysis revealed that macrophage co-culture significantly reduced long-chain free fatty acids and triacylglycerols (TGs). Quantitative correlation analysis between TGs and free fatty acids indicated that the macrophage-mediated TG reduction stemmed from decreased free fatty acid availability, the precursors for lipid synthesis. Furthermore, macrophages increased D-labeling efficiency, suggesting enhanced lipolysis contributing to TG reduction. DOLGOReQ not only facilitates relative quantification of lipid changes but also provides valuable insights into lipid turnover dynamics. These findings establish DOLGOReQ as a powerful tool for investigating global lipid metabolism changes induced by external stimuli in 3D cell culture.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128612"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural-derived sorbents: Application of biochar materials as green extractive approach in food analysis","authors":"Carla Iglesias-Martín,&nbsp;Ana M. Ares,&nbsp;José Bernal,&nbsp;Adrián Fuente-Ballesteros","doi":"10.1016/j.talanta.2025.128608","DOIUrl":"10.1016/j.talanta.2025.128608","url":null,"abstract":"<div><div>The transition toward greener methodologies in analytical chemistry has intensified interest in biochar as a sustainable sorbent for food analysis. Derived from the pyrolysis of agro-industrial residues, biochar combines low-cost production with good properties such as high surface area, porosity, and surface tunability. This review provides a critical and feedstock-oriented overview of biochar applications in food sample preparation, categorizing sorbents based on their biomass origin (fruit waste, nut and seed residues, cereal by-products, lignocellulosic fibers, and wood waste). Each source is examined in terms of its physicochemical characteristics, extraction efficiency, and performance across different food matrices. Special emphasis is placed on sorbent modification strategies, the use of environmentally compatible desorption solvents, and the alignment of biochar use with green analytical chemistry (GAC) principles. Additionally, the review identifies key research gaps, limitations in analytical reproducibility, and challenges for regulatory acceptance. Overall, biochar emerges as a versatile and eco-efficient material with strong potential to enhance sustainability in food safety analysis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128608"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asymmetrically modified hydrogel nanochannel biosensor for label-free and selective kanamycin monitoring","authors":"Lili Liu,&nbsp;Xingtong Liu,&nbsp;Linna Li,&nbsp;Yandi Hui,&nbsp;Jiajia Lu,&nbsp;Zhen Li,&nbsp;Fujun Yao,&nbsp;Xiaofeng Kang,&nbsp;YanLi Guo","doi":"10.1016/j.talanta.2025.128590","DOIUrl":"10.1016/j.talanta.2025.128590","url":null,"abstract":"<div><div>Nanochannel sensors have attracted much attention in the field of biosensing due to their unique domain-limiting effect and signal transduction mechanism. However, the traditional surface modification strategy often suffers from the low probe immobilisation efficiency, channel clogging and limited modification sites. To address these challenges, this study proposes a synergistic construction strategy combining hydrogel filling and asymmetric modification. Using polyethylene terephthalate (PET) membranes as the substrate, we constructed acrylic acid (AAc)-co-acrylamide (AAm)-co-methyl methacrylate (MMA) ternary copolymerised hydrogel within cylindrical nanochannel through free radical polymerisation reaction. Further functionalization was achieved through PEI/Zr⁴⁺ asymmetric modification, enabling precise charge gradient regulation and yielding a composite nanochannel with significant ionic current rectification (ICR) effect. As a proof of concept, kanamycin was detected with high sensitivity through aptamer recognition, triggered DNA conformational transitions, and specific coordination of Zr⁴⁺ to double-stranded DNA (dsDNA). The hydrogel's three-dimensional porous architecture significantly improves the probe loading capacity while the charge gradient optimization and the signal amplification mechanism collectively enables the sensing platform to exhibit excellent analytical performance and stability. It provides a new idea for the development of high-performance biosensors with important applications in the fields of food safety and clinical diagnosis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128590"},"PeriodicalIF":5.6,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel electrochemical approach for sensitive and simultaneous detection of 7,8-dihydro-8-oxoadenine and 2-hydroxyadenine based on a screen-printed carbon electrode modified with carbon-encapsulated Fe3O4","authors":"Yue Wang ,&nbsp;Fei Yu ,&nbsp;Qing-Hua Liu ,&nbsp;Cai-Yun Wang ,&nbsp;Guo-Yuan Zhu ,&nbsp;Li-Ping Bai ,&nbsp;Ke-Ying Guo ,&nbsp;Zhi-Hong Jiang ,&nbsp;Wei Zhang","doi":"10.1016/j.talanta.2025.128584","DOIUrl":"10.1016/j.talanta.2025.128584","url":null,"abstract":"<div><div>Excessive reactive oxygen species attack DNA, resulting in the formation of oxidative guanine (oxoG) and oxidative adenine (oxoA) as significant types of oxidative DNA damage. Despite the similar carcinogenic potential and content levels of oxoA, there is still a lack of comprehensive studies and detection methodologies compared to oxoG. Herein, a novel electrochemical approach for sensitive and simultaneous detection of 7,8-dihydro-8-oxoadenine (8-oxoA) and 2-hydroxyadenine (2-oxoA) was first reported and designed using screen-printed carbon electrode (SPCE) modified with carbon-encapsulated Fe₃O₄ (Fe₃O₄@C). The SPCE modified with Fe₃O₄@C (Fe₃O₄@C/SPCE) exhibited excellent electrocatalytic performance for the simultaneous detection of 8-oxoA and 2-oxoA without requiring any enzymes. Although 8-oxoA and 2-oxoA are a pair of isomers, their oxidation peaks could be separated using differential pulse voltammetry. Under the optimal conditions, the corresponding limits of detection were 45.3 nM and 34.5 nM for 8-oxoA and 2-oxoA (S/N = 3), demonstrating high sensitivity and a wide linear range from 0.05 μM to 100 μM. The proposed electrochemical sensor was also applied to oxoA analysis in rat serum samples with satisfactory recovery values. Overall, Fe₃O₄@C/SPCE displayed a promising oxoA assay platform, which expands the choice of biomarkers in purine oxidation and contributes to describing DNA oxidative damage more comprehensively and accurately.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128584"},"PeriodicalIF":5.6,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancement of doxorubicin monitoring with a DNA fragmentation strategy for SYBR Green I-based aptamer biosensors","authors":"Luke Wei,&nbsp;Yuxin Hu,&nbsp;Jiamin Wu,&nbsp;Jing Mao,&nbsp;Tianqin Yin,&nbsp;Jieqiong Qiu","doi":"10.1016/j.talanta.2025.128601","DOIUrl":"10.1016/j.talanta.2025.128601","url":null,"abstract":"<div><div>Doxorubicin (DOX), a widely utilized chemotherapeutic antibiotic, increasingly threatens environmental and public health due to its persistence in marine ecosystems, bioaccumulation in organisms, and contamination of agricultural systems. In response to the urgent demand for effective DOX detection, we have developed a label-free fluorescent aptamer biosensor (FAB) that employs SYBR Green I (SG I)-mediated signal amplification. A key innovation in this design is the strategic fragmentation of the 41-mer complementary strand (CP) of the DOX aptamer into three 11-mer fragments. It significantly mitigates nonspecific SG I binding interference caused by the longer ssDNA CP, enhancing the fluorescence signal ratio (F_dsDNA/F_ssDNA) from 4.6-fold to 12.6-fold. Upon DOX introduction, competitive binding between DOX, SG I, and DNA further suppresses background noise, yielding an exceptional fluorescence quenching ratio (F₀/F) of 52.8. The optimized FAB demonstrates ultra-sensitive DOX detection, with a detection limit of 1.41 nM and a linear response between 1.41 nM and 300 nM. The recoveries of DOX spiked into pond water and drinking water samples ranged from 95.1 % to 101.8 %, with relative standard deviations (RSDs) below 3.46 %, demonstrating negligible matrix effects and confirming the FAB method's robustness for environmental monitoring applications. This strategy synergistically enhances signal transduction efficiency, and strengthens detection reliability, all while maintaining operational simplicity. As a result, this FAB for DOX detection holds transformative potential, poised to revolutionize biosensor technology in the fields of food safety, environmental monitoring, and biosecurity.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128601"},"PeriodicalIF":5.6,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection, characterization and the application of the RNA aptamer in the capture assay of Erythropoietin (EPO)","authors":"Marimuthu Citartan,&nbsp;Ahmad Naqib Shuid,&nbsp;Subash C.B. Gopinath,&nbsp;Thean-Hock Tang","doi":"10.1016/j.talanta.2025.128586","DOIUrl":"10.1016/j.talanta.2025.128586","url":null,"abstract":"<div><div>Erythropoietin (EPO) is an erythropoietic growth factor that induces the production of red blood cells. However, this protein is also extensively manipulated by athletes to illegally enhance their performance, a phenomenon known as doping. Generating a feasible molecular recognition element against EPO would be beneficial for doping detection. In this study, we have successfully isolated an RNA aptamer against EPO using SELEX. Termed REPORA-6, this RNA aptamer has an estimated binding affinity of 25 ± 1 nM. REPORA-6 RNA aptamer also retains its binding against degylosylated EPO and interacts with both Epoetin Beta and Mircera, suggesting that this aptamer binds to the protein domain of the recombinant EPO despite the variation in the glycosylation side chains. Ethylnitrosourea mapping, in-line probing assay and docking revealed important nucleotides for interaction with EPO. Capitalizing these findings, a truncated RNA aptamer was derived, REPORA-6b RNA. This aptamer has an improved binding affinity of 22 ± 2 nM. We have incorporated this aptamer into a capture assay and found out that the limit of detection achieved was 19.6 pM. REPORA-6b RNA has an immense potential in applications involving doping detection of EPO.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128586"},"PeriodicalIF":6.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-targeted metabolomics and machine learning reveal metabolic dysregulation in lymph node tuberculosis","authors":"Peijun Chen ,&nbsp;Yuehui Yu ,&nbsp;Ying Zhang ,&nbsp;Gaoyi Yang","doi":"10.1016/j.talanta.2025.128583","DOIUrl":"10.1016/j.talanta.2025.128583","url":null,"abstract":"<div><h3>Background</h3><div>Lymph node tuberculosis (LNTB) is the most prevalent form of extrapulmonary tuberculosis; however, differentiating it from non-LNTB remains challenging due to overlapping clinical features and suboptimal diagnostic methods. Current diagnostic methods for LNTB lack both sensitivity and specificity. This study aimed to characterize the metabolic differences between LNTB and non-LNTB patients, elucidate the pathological mechanisms underlying LNTB, and identify diagnostic biomarkers using machine learning models.</div></div><div><h3>Methods</h3><div>Serum samples from 40 LNTB patients and 30 non-LNTB patients were analyzed using ultra-high-performance liquid chromatography-mass spectrometry. Differential metabolites were identified based on a variable importance in projection &gt;1, false discovery rate-adjusted p-value &lt;0.05. Pathway enrichment analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG). Machine learning, including support vector machines and random forest, were employed to screen for diagnostic biomarkers, which were validated by receiver operating characteristic curves.</div></div><div><h3>Results</h3><div>Among the 1294 detected metabolites, 89 exhibited significant differences between the two groups. By integrating KEGG enrichment with topological analysis, phenylalanine, tyrosine, and tryptophan biosynthesis possessed the highest impact, followed by phenylalanine metabolism, and aminoacyl-tRNA biosynthesis. Machine learning identified four biomarkers: Leu-Ala [area under the curve (AUC) = 0.8292], evodiamine (AUC = 0.7558), fenazaquin (AUC = 0.7175), and acetol (AUC = 0.7117). Leu-Ala demonstrated the highest diagnostic accuracy, with a sensitivity of 73.5 % and specificity 86.7 % at a cutoff value of 0.62.</div></div><div><h3>Conclusions</h3><div>Untargeted metabolomics revealed dysregulation in the biosynthesis of phenylalanine, tyrosine, and tryptophan, phenylalanine metabolism, as well as in aminoacyl-tRNA biosynthesis in LNTB. Additional, Leu-Ala was identified as a novel diagnostic biomarker. The integrating of metabolomics with machine learning presents a promising approach for LNTB detection, though larger validation studies are necessary.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128583"},"PeriodicalIF":5.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}