Talanta最新文献_第10页

Cyclodextrin mediated non-aqueous electrophoresis for chiral separations of anionic boron clusters. 环糊精介导的非水电泳手性分离阴离子硼簇。

IF 5.6 1区化学

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-10 DOI: 10.1016/j.talanta.2025.128117

Ondřej Horáček, Lukáš Lochman, Kristýna Linková, Ece Zeynep-Tüzün, Bohumír Grüner, Radim Kučera

{"title":"Cyclodextrin mediated non-aqueous electrophoresis for chiral separations of anionic boron clusters.","authors":"Ondřej Horáček, Lukáš Lochman, Kristýna Linková, Ece Zeynep-Tüzün, Bohumír Grüner, Radim Kučera","doi":"10.1016/j.talanta.2025.128117","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128117","url":null,"abstract":"Anionic boron cluster compounds have recently made their way into many areas including medicinal chemistry and sensors due to favorable physical-chemical properties and their various biological activity. Notwithstanding the inherent chirality of these compounds, the exploration of the properties and activity of individual enantiomers remains uncharted territory. The permanent delocalized negative charge enables the electrophoretic mobility of these compounds. Thus, chiral electrophoresis, characterized by minimal consumption of chemicals and a sample, emerges as a promising candidate for a reliable quality control tool. The primary attempts in aqueous electrolytes showed some difficulties related to the limited solubility of these analytes. This study meticulously investigates the electrophoretic behavior and chiral separation of anionic [7,8-nido-C2B9H11]- and cobalt bis(dicarbollide)(1-) derivatives using a methanolic non-aqueous electrolyte with numerous derivatives of cyclodextrins. Randomly substituted hydroxypropyl-β-, methyl-β-, and hydroxypropyl-γ-cyclodextrins were identified as the most effective chiral selectors. The chiral separations delineated herein surpass previously published results in capillary electrophoresis in terms of resolution, peak shape, and the number of theoretical plates. Furthermore, the application of (2-hydroxy-3-N,N,N-trimethylamino)propylated β-cyclodextrin in non-aqueous environment resulted in the chiral separation of seven recently synthesized amino cobalt bis(dicarbollide)(1-) derivatives; thereby, reinforcing the extensive applicability of the developed methodology for different structural types of anionic cobalt bis(dicarbollides)(1-). These results qualify non-aqueous electrophoresis as a valuable tool for the enantiomeric purity control of anionic boron cluster compounds with respect to their further use in various areas.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128117"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bipedal DNA walker integrated resonance energy transfer to construct a sensitive electrochemiluminescence biosensor for serotonin detection. 双足DNA行走器集成共振能量转移构建了灵敏的电化学发光生物传感器用于血清素检测。

IF 5.6 1区化学

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-17 DOI: 10.1016/j.talanta.2025.128175

Hongyuan Li, Yan Jiao, Yibing Shi, Qiumei Feng, Yongguang Gao

{"title":"Bipedal DNA walker integrated resonance energy transfer to construct a sensitive electrochemiluminescence biosensor for serotonin detection.","authors":"Hongyuan Li, Yan Jiao, Yibing Shi, Qiumei Feng, Yongguang Gao","doi":"10.1016/j.talanta.2025.128175","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128175","url":null,"abstract":"The abnormal level of neurotransmitter serotonin often leads to the blood-brain barrier. Thus, it is urgent to develop a sensitive and efficient method for serotonin detection. Here, we constructed a resonance energy transfer-based electrochemiluminescence (ECL-RET) biosensor sensitized with bipedal DNA walker for the sensitive determination of serotonin. Benefitting from the specific recognition of aptamer to target and enrichment ability of magnetic bead separation, this system achieved the signal transduction of serotonin to nucleic acid and excellent selectivity for serotonin analysis among various analogues. The spectral overlap between the emission spectrum of CdS QDs (energy donor) and the absorption of Ag nanoclusters (Ag NCs, energy acceptor) enabled effective ECL-RET. Through proximity ligation, bipedal DNA walker driven by catalytic hairpin assembly (CHA) significantly accelerated the reaction kinetics and resulted in the amplified responses. Relying on the ECL quenching, serotonin was quantified with a linear detection range of 1 pM-1 μM and a low detection limit of 0.28 pM. More notably, the practical application of the sensor was tested in human serum with recovery rates ranging from 95.0 % to 105.7 %. The combination of ECL-RET, proximity ligation and CHA-driven bipedal DNA walker enabled the biosensor to represent a step forward in neurological-related disease diagnosis.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128175"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a CRISPR/Cas12a-assisted fluorescent aptasensor for simultaneous detection of zearalenone and ochratoxin A 同时检测玉米赤霉烯酮和赭曲霉毒素a的CRISPR/ cas12a辅助荧光适配体传感器的建立

IF 6.1 1区化学

Talanta Pub Date : 2025-10-01 DOI: 10.1016/j.talanta.2025.128937

Ling Li , Menglei Li , Sai Wang , Yiyang Dong

{"title":"Development of a CRISPR/Cas12a-assisted fluorescent aptasensor for simultaneous detection of zearalenone and ochratoxin A","authors":"Ling Li , Menglei Li , Sai Wang , Yiyang Dong","doi":"10.1016/j.talanta.2025.128937","DOIUrl":"10.1016/j.talanta.2025.128937","url":null,"abstract":"<div><div>Mycotoxins, such as zearalenone (ZEN) and ochratoxin A (OTA), represent significant hazards to both human and animal health, necessitating strict monitoring and regulation of mycotoxin levels in food, feed, and environment. In this study, a simple and efficient CRISPR/Cas12a-assisted fluorescent aptasensor is presented for the simultaneous detection of ZEN and OTA. Utilizing a Bifunctional aptamer (B-APT), this biosensor achieves dual recognition of the both targets, subsequently converting their concentrations into observable fluorescent signals through the Cas12a/crRNA cis-cleavage activity. Rational modifications of the complementary strands specific to the two targets enable distinct emission wavelengths under the same excitation light, facilitating simultaneous and independent quantitative determination of ZEN and OTA. Under optimized conditions, the CRISPR/Cas12a-aptasensor demonstrates robust detection capabilities for individual ZEN and OTA targets, as well as their mixture, yielding consistent standard curves. This methodology exhibits reliable detection of ZEN and OTA concentrations spanning from 0.25 nM to 256 nM and 1 nM to 1024 nM, with respective limit of detection (LOD) values of 190 pM and 931 pM. Furthermore, this method showcases exceptional selectivity and considerable recovery rates (89.17 %–109.88 % for ZEN and 101.19 %–106.51 % for OTA) in corn oil samples, underscoring its efficacy as an advanced tool for ZEN and OTA detection and offering valuable insights into the simultaneous detection of diverse targets.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 ","pages":"Article 128937"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

l(+)-tartaric acid of grape origin: Definition of threshold limits for the ¹³C/¹²C and ¹⁸O/¹⁶O stable isotope ratios and validation of the isotopic method through an interlaboratory study. 葡萄原产l(+)-酒石酸：13C/12C和18O/16O稳定同位素比值阈值的定义及实验室间同位素方法的验证

IF 5.6 1区化学

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-02 DOI: 10.1016/j.talanta.2025.128071

Matteo Perini, Silvia Pianezze, Laura Pienti, Giovanna Randi, Pietro Franceschi, Roberto Larcher

{"title":"l(+)-tartaric acid of grape origin: Definition of threshold limits for the 13C/12C and 18O/16O stable isotope ratios and validation of the isotopic method through an interlaboratory study.","authors":"Matteo Perini, Silvia Pianezze, Laura Pienti, Giovanna Randi, Pietro Franceschi, Roberto Larcher","doi":"10.1016/j.talanta.2025.128071","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128071","url":null,"abstract":"l(+)-tartaric acid is one of the most used additives in the oenological practices. Its properties make it useful as preservative and acidulant in grape-derived products. This additive can be obtained through the extraction from grapes, from other fruits or through synthesis from petrochemical sources. In Europe, only l(+)-tartaric acid from grapes can be added to wine products, but the price difference between the forms could lead to the fraudulent use of the cheaper synthetic l(+)-tartaric acid. In other continents where such limitation is not applied, the clear commercial identification of the products is still desirable. Stable isotope analysis of carbon (δ13C) and oxygen (δ18O) proved to be a robust and effective method in discriminating between synthetic and grape-derived l(+)-tartaric acid. In this study, to define the threshold limits of δ13C (above -24.8 ‰) and δ18O (above +25.6 ‰) of grape-derived l(+)-tartaric acid, a database consisting of 81 authentic natural samples was created. Moreover, the validation of the isotopic method through an interlaboratory study involving nine European and extra-European laboratories, was provided. The repeatability and the reproducibility standard deviation of the method were calculated.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128071"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel system for precise identification and explainability analysis based on multimodal learning combining laser-induced breakdown spectroscopy and laser-induced plasma acoustic signals. 结合激光诱导击穿光谱和激光诱导等离子体声信号的基于多模态学习的精确识别和可解释性分析新系统。

IF 5.6 1区化学

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-17 DOI: 10.1016/j.talanta.2025.128182

Wenhan Gao, Boyuan Han, Zhuoyi Sun, Yihui Yan, Yanpeng Ye, Jun Feng, Yuyao Cai, Asiri Iroshan, Yuzhu Liu

{"title":"A novel system for precise identification and explainability analysis based on multimodal learning combining laser-induced breakdown spectroscopy and laser-induced plasma acoustic signals.","authors":"Wenhan Gao, Boyuan Han, Zhuoyi Sun, Yihui Yan, Yanpeng Ye, Jun Feng, Yuyao Cai, Asiri Iroshan, Yuzhu Liu","doi":"10.1016/j.talanta.2025.128182","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128182","url":null,"abstract":"This study presents an innovative approach to identify copper types using Laser-Induced Breakdown Spectroscopy (LIBS) in conjunction with Laser-Induced Plasma Acoustic (LIPA) signals. Traditionally overlooked, plasma acoustic signals can indeed provide valuable insights into plasma characteristics essential for copper identification. This study pioneers a cross-modal learning technique, integrating LIBS and LIPA signals, and employs a Support Vector Machine (SVM) for classification. To enhance feature extraction, Principal Component Analysis (PCA) reduces data dimensionality, while SHapley Additive exPlanations (SHAP) assess feature contributions, aiding feature selection. The combined model demonstrates high identification accuracy, and the interpretability analysis deepens our understanding of feature roles in copper detection. This framework not only boosts LIBS-based identification accuracy but also advances the theoretical foundation for multi-modal data fusion in material analysis.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128182"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of total- and water-soluble chloride in concrete using an optical sensor. 用光学传感器分析混凝土中总氯和水溶性氯。

IF 5.6 1区化学

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-11 DOI: 10.1016/j.talanta.2025.128124

Karl L Sterz, Bernhard Müller, Marlene Sakoparnig, Isabel Galan, Andreas Steinegger, Cyrill Grengg, Florian Mittermayr, Sergey M Borisov, Torsten Mayr

{"title":"Analysis of total- and water-soluble chloride in concrete using an optical sensor.","authors":"Karl L Sterz, Bernhard Müller, Marlene Sakoparnig, Isabel Galan, Andreas Steinegger, Cyrill Grengg, Florian Mittermayr, Sergey M Borisov, Torsten Mayr","doi":"10.1016/j.talanta.2025.128124","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128124","url":null,"abstract":"An optical chloride-sensitive film was prepared and utilized to measure the chloride content in powdered concrete samples. The sensor is composed of N,N'-dimethyl-9,9'-biacridiniumnitrat (Lucigenin), a phosphorescent ruthenium complex in dense silica microparticles and a custom-made poly(acrylonitrile-co-acrylamide) matrix, allowing utilization of the dual lifetime referencing (DLR) technique. No dependency on pH value was observed at pH values from 1.0 to 7.0. Among the relevant anions, the sensor did not show any cross-talk to sulphate, but the response towards chloride was reduced in presence of nitrate and acetate ions. The sensor remains stable in water and 50 % acetic acid, which enables measurements of water-soluble chloride and total chloride. The sensor was challenged with concrete samples of various origins and with strong differences in chloride contamination (0.02-0.38 %w/w). The results of the optical measurements are in excellent agreement with results obtained from potentiometric titrations. The divergent binder chemistry of the selected concrete samples and the reliability of the measured results suggest wide applicability of the sensor. The sensor also shows potential to be suitable for field measurements.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128124"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoengineered hybrid hydrogel-based 3D nanocomposite as an antifouling coating interface for enhanced electrochemical aptasensing of estradiol. 纳米工程杂化水凝胶基三维纳米复合材料作为增强雌二醇电化学适配的防污涂层界面。

IF 5.6 1区化学

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-09 DOI: 10.1016/j.talanta.2025.128105

Gopi Karuppaiah, Aneesh Koyappayil, Sachin Ganpat Chavan, Anna Go, Hwang Seowoo, Pooja Ramrao Rathod, SangWook Lee, Min-Ho Lee

{"title":"Nanoengineered hybrid hydrogel-based 3D nanocomposite as an antifouling coating interface for enhanced electrochemical aptasensing of estradiol.","authors":"Gopi Karuppaiah, Aneesh Koyappayil, Sachin Ganpat Chavan, Anna Go, Hwang Seowoo, Pooja Ramrao Rathod, SangWook Lee, Min-Ho Lee","doi":"10.1016/j.talanta.2025.128105","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128105","url":null,"abstract":"Electrochemical biosensing has emerged as a promising method for the point-of-care detection of various biomarkers. However, its clinical application faces significant challenges due to biofouling when exposed to clinical samples. This study presents a novel nanocomposite coating aimed at addressing biofouling in electrochemical biosensors by developing an Electrochemical Estradiol Aptasensor (EAS). We developed a nanoengineered, hybrid hydrogel-based 3D antifouling nanocomposite interface (ANcI) by crosslinking carboxymethyl chitosan with sodium carboxymethyl cellulose and incorporating highly conductive Ti3C2Tx MXene nanostructures. The Aptasensor was constructed on a screen-printed carbon electrode by applying the ANcI as an antifouling layer, followed by the deposition of a gold nanoparticle support layer modified with estradiol-specific aptamers, which serves as the biorecognition element. We evaluated the antifouling capabilities by comparing the performance of the Aptasensor with and without the ANcI when exposed to human serum and bovine serum albumin. This innovative nanocomposite coating offers excellent antifouling properties along with a highly porous structure and electrical conductivity, which are essential for maintaining sensor performance in complex clinical samples. By addressing the limitations of existing antifouling materials, this approach paves the way for the commercialization of electrochemical biosensors with enhanced accuracy and sensitivity. The sensors demonstrate a clinically relevant concentration range of 0.1 pg/mL to 1000 pg/mL, with a limit of detection of 0.127 pg/mL. These findings highlight the potential of this innovative approach to improve electrochemical biosensing across various applications and significantly impact biomarker detection in clinical samples.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128105"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of plasma radiation attenuation rate on the uncertainty of LIBS signal and real-time correction method. 等离子体辐射衰减率对LIBS信号不确定度的影响及实时校正方法。

IF 6.1 1区化学

Talanta Pub Date : 2025-10-01 DOI: 10.1016/j.talanta.2025.128940

Chao Li, Tao Zhang, Yaru Li, Runhao Li, Xuan Liu, Caijie Liu, Zhongyi Bao, Qibin Zhang, Yunfeng Bi

{"title":"Effect of plasma radiation attenuation rate on the uncertainty of LIBS signal and real-time correction method.","authors":"Chao Li, Tao Zhang, Yaru Li, Runhao Li, Xuan Liu, Caijie Liu, Zhongyi Bao, Qibin Zhang, Yunfeng Bi","doi":"10.1016/j.talanta.2025.128940","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128940","url":null,"abstract":"The integration process of the spectrometer detector is a critical step in Laser-Induced Breakdown Spectroscopy (LIBS) signal acquisition. However, the influence of plasma radiation attenuation during the integration time on LIBS uncertainty remains unclear. In this study, plasma emission was simultaneously monitored using a combined detection system consisting of an echelle spectrometer with an intensified charge-coupled device (ICCD) and a monochromator with a photomultiplier tube (PMT). Based on this setup, the effect of radiation attenuation rate on spectral line intensity was investigated, and a real-time uncertainty correction method using the attenuation rate was developed. Results show that in repeated independent measurements of a homogeneous sample, the radiation attenuation rate at any wavelength is inversely correlated with spectral line intensity. In contrast, for samples with varying contents of the same element, no statistically significant correlation was found between attenuation rate and elemental content. These findings indicate that the radiation attenuation rate is indeed a factor contributing to LIBS signal uncertainty. Moreover, using radiation attenuation rates as correction coefficients for spectral line intensities reduced the relative standard deviation (RSD) of repeated measurements and improved the coefficient of determination (R2) of calibration curves for gradient-content samples. This work clarifies the relationship between plasma radiation attenuation rate and line intensity, and examines its impact on repeatability and quantitative analysis. The results further refine existing theories and provide valuable insights for the theoretical development and broader application of LIBS technology.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt B","pages":"128940"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graphitic carbon nitride/1-pyrenebutyric acid N-hydroxysuccinimide/polythiophene nanocomposite photoelectrochemical biosensor for CA 19-9 detection. 用于CA 19-9检测的石墨氮化碳/1-芘丁酸n -羟基琥珀酰亚胺/聚噻吩纳米复合光电化学生物传感器。

IF 5.6 1区化学

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-19 DOI: 10.1016/j.talanta.2025.128065

Adriel Yan Sheng Tan, Faliang Cheng, Min Zhang, Michelle T T Tan, Sivakumar Manickam, Kasturi Muthoosamy

{"title":"Graphitic carbon nitride/1-pyrenebutyric acid N-hydroxysuccinimide/polythiophene nanocomposite photoelectrochemical biosensor for CA 19-9 detection.","authors":"Adriel Yan Sheng Tan, Faliang Cheng, Min Zhang, Michelle T T Tan, Sivakumar Manickam, Kasturi Muthoosamy","doi":"10.1016/j.talanta.2025.128065","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128065","url":null,"abstract":"A photoelectrochemical (PEC) biosensor composed entirely of carbon nanomaterials was synthesized to detect carbohydrate antigen 19-9 (CA 19-9). The biosensor platform integrated graphitic carbon nitride (GCN), known for its light sensitivity, polythiophene (PTh), an organic conductive and optically active material, and 1-pyrenebutyric acid N-hydroxysuccinimide (PBASE), which functions both as a biolinker to conjugate CA 19-9 antibody and antigen and as an electron mediator to facilitate electron transfer from GCN to PTh. The formation of a Schottky heterojunction between PTh and GCN reduced the bandgap of GCN from 2.66 to 1.96 eV, which enhanced transfer of photogenerated electrons for cathodic photocurrent generation. The improvement of charge transfer due to heterojunction formation and π-π stacking between GCN and the pyrene group of PBASE is confirmed by cyclic voltammetry (CV), electron impedance spectroscopy (EIS), and chronoamperometry (CA) findings. The highest current of 1.31 μA is observed for combination of 5 wt% PTh with a GCN/PBASE ratio of 1:0.5. Besides evaluating the electron mobility of GCN/PBASE/PTh, CV, EIS, and CA were also used to evaluate the sensor performance. Optimization studies revealed that 0.6 μg of CA 19-9 antibody and 1 h of antigen-antibody immobilization time significantly improved the biosensor response. The GCN/PBASE/PTh biosensor demonstrated high sensitivity to CA 19-9 antigen across a concentration of 50-1000 U/ml and a detection limit as low as 0.052 U/ml. The reported working range is within the limits required for diagnostic testing of patients with hepatic and heart problems as well as for post-treatment monitoring of colorectal and pancreatic cancer patients.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128065"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A smartphone-integrated CuFe aerogel nanozyme for rapid and visual detection of ascorbic acid in real samples. 一种集成智能手机的CuFe气凝胶纳米酶，用于快速和视觉检测实际样品中的抗坏血酸。

IF 5.6 1区化学

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-15 DOI: 10.1016/j.talanta.2025.128169

Lijun Mao, Wenhua Bi, Jianqi Ye, Xinhao Wan, Yarui Tang, Yao Chen, Wei Liu, Dan Wen

{"title":"A smartphone-integrated CuFe aerogel nanozyme for rapid and visual detection of ascorbic acid in real samples.","authors":"Lijun Mao, Wenhua Bi, Jianqi Ye, Xinhao Wan, Yarui Tang, Yao Chen, Wei Liu, Dan Wen","doi":"10.1016/j.talanta.2025.128169","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128169","url":null,"abstract":"The development of highly active nanozymes for advanced bioanalysis has attracted considerable attention, yet significant challenges remain. Inspired by the structural features of natural oxidases with transition metal active sites, we designed a bimetallic CuFe aerogel with significantly enhanced oxidase-like activity for the facile and visual detection of ascorbic acid (AA). The incorporation of Fe into the Cu aerogel matrix resulted in a remarkable 7.8-fold increase in oxidase-like activity compared to pure Cu aerogel, as confirmed by comprehensive experimental analyses and theoretical calculations. Leveraging the inhibitory effect of AA on the Cu6Fe aerogel-catalyzed oxidation reaction, we developed a rapid and sensitive colorimetric detection method, achieving a low detection limit of 0.1 μM. Furthermore, by integrating the Cu6Fe aerogel with portable test strips and smartphone-based colorimetric analysis, we constructed a visual sensing platform capable of accurate and reliable AA quantification. The practical applicability of this platform was successfully demonstrated through the detection of AA in real-world samples, including fruits and commercial vitamin C beverages. This work not only provides a novel strategy for designing highly active metal aerogel-based nanozymes but also opens new avenues for their application in visual sensing and point-of-care diagnostics.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128169"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0