Talanta最新文献

{"title":"Comprehensive analysis strategy of quality for traditional Chinese medicine compound based on fingerprint technology and quantitative prediction of spectra","authors":"Ming Cai ,&nbsp;Siqi Wang ,&nbsp;Jiayi Jiang ,&nbsp;Kai Gao ,&nbsp;Guoxiang Sun","doi":"10.1016/j.talanta.2025.127661","DOIUrl":"10.1016/j.talanta.2025.127661","url":null,"abstract":"<div><div>Traditional Chinese medicine compound (TCMC) comes from a wide range of sources, and the complexity of chemical composition leads to differences in quality. Improving quality evaluation standards is the focus as well as the difficulty in its development and application. The traditional fingerprint analysis method has certain limitations for the quality evaluation of TCMC and the judgment of superior and inferior products. Therefore, how to optimize the similarity evaluation method of fingerprint and mine the sample data on the basis of fingerprint to fully reflect the essential differences in the quality of TCMC is the focus of this paper. In this paper, the quantified fingerprint method of integral efficiency (IEQFM) combined with dual control of peak area and retention time was proposed to optimize the similarity evaluation method. The HPLC, GC and UV fingerprints of 35 batches of compound liquorice tablets (CLTs) were evaluated as an example. The offline-wavelength switching method was explored to simplify the multi-wavelength data processing method. Secondly, the 11 components in the CLTs were accurately quantitatively analyzed by the single-linear assay multi-markers method and the standard curve method. Orthogonal partial least squares-discriminant analysis (OPLS-DA) analysis was used to find the signature components affecting the quality difference. For UV spectra, quantum fingerprints were obtained by preprocessing using characteristic concatenation method and quantitatively predicted using the partial least squares regression (PLSR) model (<em>p</em> &gt; 0.7). Finally, the three fingerprint profiles were fused and evaluated after assigning weights to the different evaluation methods using the criteria importance through intercriteria correlation (CRITIC) method. In order to adapt to the evolving TCMC, method optimization is of great significance to improve the quality evaluation criteria. This study provides an exploratory method for the quality analysis of TCMC.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127661"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-sensitivity flexible electrochemical sensor for real-time multi-analyte sweat analysis","authors":"Yan Zhang,&nbsp;Yining Sun,&nbsp;Jingxuan Han,&nbsp;Ming Zhang,&nbsp;Fangjie Li,&nbsp;Dongye Yang","doi":"10.1016/j.talanta.2025.127644","DOIUrl":"10.1016/j.talanta.2025.127644","url":null,"abstract":"<div><div>Flexible sweat sensors play a crucial role in health monitoring and disease prevention by enabling real-time, non-invasive assessment of human physiological conditions. Sweat contains a variety of biomarkers, offering valuable insights into an individual's health status. In this study, we developed an advanced flexible electrochemical sensor featuring reduced graphene oxide (rGO)-based electrodes, modified with a composite material comprising nitrogen and sulfur co-doped holey graphene (HG) and MXene, with in-situ-grown TiO₂ nanoparticles on the MXene. The sensor design leverages the synergistic properties of its components: MXene provides a conductive scaffold, TiO₂ enhances electrocatalytic activity, and the porous HG network facilitates efficient ion and electron transfer, with doping increasing the number of active sites. This configuration enables sensitive and simultaneous detection of ascorbic acid (AA), uric acid (UA), and dopamine (DA). Additionally, a potassium-selective (K⁺) film applied to rGO-enhanced electrodes supports concurrent detection of K⁺ in sweat. The sensor array demonstrates a broad detection range, low detection limits, and high sensitivity, while maintaining mechanical flexibility, anti-interference capabilities, and repeatability. Validated through in-situ sweat biomarker detection during exercise, the sensor effectively tracked fluctuations in K⁺, AA, and UA levels in a volunteer. This practical application underscores the sensor's potential for continuous health monitoring, and early disease detection, establishing it as a promising tool in modern healthcare.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127644"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reliable and specific biosensing on single- and double-stranded aptamer functionalized remote dual-gate organic field-effect transistors: A comparison","authors":"Xiaoyan Chen ,&nbsp;Mingshuang Li ,&nbsp;Xinyu Yang ,&nbsp;Jiangdong Cui ,&nbsp;Jiacheng Ge ,&nbsp;Ying Liu ,&nbsp;Mengtao Ma","doi":"10.1016/j.talanta.2025.127634","DOIUrl":"10.1016/j.talanta.2025.127634","url":null,"abstract":"<div><div>Organic field-effect transistors (OFETs) integrated with commercial transistors are promising sensing platforms characterized by enhanced device uniformity, functional diversity, and electrical output stability. Aptamers with charged backbones and a high affinity for target molecules are anticipated to mitigate the limitations imposed by Debye screening in physiological environments with high ionic strength, thereby facilitating specific biological recognition in complex surroundings. This study presents two reliable OFET aptasensors for vascular endothelial growth factor (VEGF) and offers a systematic comparison of their performance. The poly[3-(3-carboxypropyl) thiophene-2,5-diyl] (PT-COOH) transducer films on remote dual gates were functionalized with VEGF aptamer (VEap), either alone or in conjunction with its complementary single-stranded DNA (PT-VEap vs. PT-VEap/CS). The single-stranded and double-stranded aptamer-based sensors exhibited opposite changes in threshold voltage in response to VEGF, and the contribution of CS pairing to signaling was evidenced by the lower detection limit of PT-VEap/CS sensors (0.1 ng/mL) compared to that of PT-VEap sensors (1 ng/mL). PT-VEap sensors demonstrated a faster response, while PT-VEap/CS sensors provided more stable and reliable signals. Both sensors maintained high sensitivity to VEGF across a broad pH range (5–9) and ionic strength (0.05–1.0 × PBS), with a slight advantage for PT-VEap in pH resistance and for PT-VEap/CS in salt resistance. The observed differences in sensing performance can be elucidated through the field effect and double electrical layer models. Additionally, the real-time responses and specific molecular recognition of VEGF were analyzed. This study quantifies the differences between single- and double-stranded aptamer-functionalized OFET biosensors, providing foundational data for the design of cost-effective and high-performance FET aptasensors applicable in various real-world scenarios.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127634"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The rational design of europium complex based electrochemiluminescence sensor for highly efficient carbaryl detection","authors":"Shan Zhang ,&nbsp;Yuexing Zhang ,&nbsp;Meng Yuan ,&nbsp;Han Liang ,&nbsp;Nuo Zhang ,&nbsp;Zhen Zhou ,&nbsp;Xia Sun ,&nbsp;Yemin Guo ,&nbsp;Feng Tang ,&nbsp;Tianyan You ,&nbsp;Daopeng Zhang","doi":"10.1016/j.talanta.2025.127659","DOIUrl":"10.1016/j.talanta.2025.127659","url":null,"abstract":"<div><div>To address the issue that most of the reported electrochemiluminescence (ECL) sensors are usually subjected to various complicated chemical modifications, we proposed a sensitive ECL sensor by using a neutral mononuclear lanthanide metal europium complex Eu(L)₃ (L = 2-pyrazol-1-yl-6-(1H-tetrazol-5-yl)-pyridine) as luminophore with simple chemical composition for the accurate detection of carbaryl pesticide residues. The three conjugated tridentate pyrazo-pyridine- tetrazol ligands could put significant effect on the physical and chemical behavior of the central Eu³⁺ ion, leading to the excellent luminescent performance of the Eu(L)₃ complex enhanced by “antenna effect” of the auxiliary anionic organic ligands. The naphthol obtained from the hydrolysis reaction of carbaryl in an alkaline working solution could effectively and quantitatively quench the luminescence intensity of the Eu(L)₃ complex, which has been theoretically confirmed by the detailed density functional theory (DFT) and time-dependent DFT (TDDFT) calculations, thus obviously strengthening the target detection capability on carbaryl. Based-on such the idea, the skillfully constructed ECL sensor showed superior carbaryl detection performance with good linearity in the range of 1 pg/mL to 1000 ng/mL, and a satisfied detection limit of 8.8 pg/mL (S/N = 3) under the optimized conditions. The current simple ECL carbaryl detection platform exhibited the conspicuous superiority to those existed commercial ones and thus, more importantly, provided a very promising practical value in the field of environmental detection (oil, soil, water, etc.) of organic phenol pollutants.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127659"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AuNCs@ZIF-8 with enhanced AIE effect to develop a lateral flow immunosensor for POC dual-modal detection of acetochlor","authors":"Pengyue Song ,&nbsp;Doudou Lei ,&nbsp;Lingling Li ,&nbsp;Nan Long ,&nbsp;Qingbin Xu ,&nbsp;Ying Li ,&nbsp;Lidong Zhou ,&nbsp;Ruile Pan ,&nbsp;Weijun Kong","doi":"10.1016/j.talanta.2025.127649","DOIUrl":"10.1016/j.talanta.2025.127649","url":null,"abstract":"<div><div>Regarding the high incidence of residue and serious toxicity of acetochlor (ATC) pesticide, it is urgent to explore simple and low-cost methods for their sensitive detection in food. In this study, with zeolite-like imidazole frameworks (ZIFs) as the carriers for embedding glutathione-modified luminescent gold nanoclusters (GSH-AuNCs) exhibiting aggregation-induced emission (AIE) effect, a GSH-AuNCs@ZIF-8 probe driven strip-based lateral flow immunosensor (LFIS) was developed for visual qualitative and fluorescent quantitative dual-modal detection of ATC. The encapsulation of GSH-AuNCs into ZIF-8 with restriction effect not only significantly improved the stability and anti-interference ability of AuNCs, but also remarkably enhanced the fluorescence efficiency for signal amplification due to the enhanced AIE effect of GSH-AuNCs, endowing the LFIS platform with unique performance. Under optimal conditions, the LFIS platform could realize the point-of-care visualization and quantitation of ATC within 20 min with a detection limit of 8.3 pg/mL and a broad linear range of 0.01–10.0 ng/mL. High recoveries of 96.44%–106.8 % in the spiked ginger samples confirmed the outstanding accuracy and dependability of the new platform for ATC analysis of real samples. Compared with other immunoassays, the AuNCs@ZIF-8-based dual-modal LFIS exhibited the advantages of simple preparation, simple operation, short analytical time, and low cost, which could be expanded to monitor more pesticides and more trace contaminants in diverse food and agricultural products, and medicinal material matrices.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127649"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new ternary deep eutectic solvent for electropolymerization of thionine on glassy carbon electrodes modified with multiwalled carbon nanotubes for the determination of ascorbic acid","authors":"Zeferino S.B. Pedro,&nbsp;Joseany M.S. Almeida,&nbsp;Christopher M.A. Brett","doi":"10.1016/j.talanta.2025.127653","DOIUrl":"10.1016/j.talanta.2025.127653","url":null,"abstract":"<div><div>The phenazine redox dye, thionine (Th), was used to prepare poly(thionine) (PTh) polymer film modified electrodes. PTh was electrodeposited on glassy carbon electrodes (GCE) and on GCE modified with multiwalled carbon nanotubes (MWCNT) by potential cycling electropolymerization in the ternary deep eutectic solvent composed of choline chloride (ChCl), thymol (Thy), ethylene glycol (EG), ChCl-Thy-EG (1:1:4) with the addition of different doping acids. The MWCNT promote greater polymer growth, conductivity and increased stability of the polymer films. The modified electrodes, PTh/MWCNT/GCE, were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and the morphology of its surface by scanning electron microscopy. PTh/MWCNT/GCE was used in the determination of ascorbic acid (AA) using fixed potential amperometry with a linear range of 2.5 μM–100 μM and a detection limit of 0.68 μM. The proposed sensor was tested on commercial pharmaceutical samples successfully with good sensitivity, repeatability and reproducibility.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127653"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic field facilitated-colorimetric aptasensor for selective and sensitive detection of Staphylococcus aureus using aptamer-labeled Zn/Co ICPs nanoenzyme","authors":"Siwei Zhou ,&nbsp;Peipei Qiu ,&nbsp;Ruihua Feng ,&nbsp;Junhe Zhang ,&nbsp;Zhengquan Su ,&nbsp;Yan Bai ,&nbsp;Jincan He","doi":"10.1016/j.talanta.2025.127633","DOIUrl":"10.1016/j.talanta.2025.127633","url":null,"abstract":"<div><div>Colorimetric detection of pathogenic bacteria (such as <em>S. aureus</em>) in complex sample confronts challenges regarding sensitivity, selectivity, and accuracy. In this paper, a magnetic field facilitated (MFF)-colorimetric aptasensor was proposed for <em>S. aureus</em> detection based on magnetic separation and nanoenzyme-facilitated chromogenic reaction. The vancomycin-functionalized magnetic chitosan (CS-MNPs-Van) was introduced to capture and isolate <em>S. aureus</em> from the complex matrix through magnetic field separation. The zinc/cobalt infinite coordination polymers (Zn/Co ICPs) was rapidly prepared with a green synthesis technology at room temperature. It was directly functionalized with aptamer through interactions of Zn²⁺/Co²⁺ and phosphate backbone, large surface area and π-π stacking reactions, avoiding the tedious chemical modification. The aptamer-labeled Zn/Co ICPs (Zn/Co ICPs-Apt) exhibited not only the excellent peroxidase-like activity, but also the good specificity toward the captured <em>S. aureus</em> to generate CS-MNPs-Van@<em>S. aureus</em>@Zn/Co ICPs-Apt complexes. The residual unreacted Zn/Co ICPs-Apt catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue oxTMB, which can be distinguished with naked eyes. The <em>S. aureus</em> concentration showed a negative correlation with the absorbance (<em>A</em>_{652 nm}) in the range of 1.8 × 10¹ to 1.8 × 10⁵ CFU/mL, with a detection limit of 4 CFU/mL. The MFF-colorimetric aptasensor presents advantages of high sensitivity, good selectivity, low matrix interference and naked visualization without complicated devices, making it competitive candidate for on-site and large-scale detection of pathogens.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127633"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A label-free fluorescence aptasensor for salivary exosomes based on a nano-micro dual-scale signal amplification strategy","authors":"Lu-Shuang Li ,&nbsp;Xiao-Hao Chen ,&nbsp;Yu Pei ,&nbsp;Xiao-Lin Xie ,&nbsp;Cong-Ting Wu ,&nbsp;Shuang Cai ,&nbsp;Jing Li ,&nbsp;Wei Gong","doi":"10.1016/j.talanta.2025.127638","DOIUrl":"10.1016/j.talanta.2025.127638","url":null,"abstract":"<div><div>Oral squamous cell carcinoma (OSCC) is the most prevalent histological subtype of oral cancer and urgently requires a noninvasive approach for timely detection to improve patients’ prognose. Salivary exosome act as a promising biomarker, while the complex and expensive analytical methods impeded its popularization in clinical applications. In this study, we developed a label-free aptasensor for salivary exosomes that utilizes a “one exosome to multiple carbon dots” signal conversion method along with nano-micro dual-scale signal amplification strategy. SiO₂ microspheres served as substrates, while carbon dots acted as luminophores, with CD63 aptamers functioning as selective recognition elements. Exosomes compete to bind with the aptamers, thereby leading to the release of non-specifically adsorbed carbon dots from the substrates. The concentration of exosomes is proportional to the variation in fluorescence intensity, enabling highly sensitive detection in artificial saliva samples, with a favorable linearity range of 2.5 × 10²∼5 × 10⁸ particles/mL and a relatively low LOD of 100 particles/mL. The anti-interference ability is deemed acceptable, and the fabrication process is affordable coupled with a straightforward signal output mode. The aptasensor was applied successfully to distinguish OSCC patients from healthy individuals, suggesting its potential for screening OSCC in physical examination centers and communities.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127638"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A split-type near-infrared photoelectrochemical and colorimetric dual-mode biosensor for the high-performance determination of HepG2 cells","authors":"Yawen Hong ,&nbsp;Lian Xu ,&nbsp;Hengwei Sun ,&nbsp;Wen Wu ,&nbsp;Xiaojun Cai ,&nbsp;Qingfeng Lin ,&nbsp;Xiaoyang Chen ,&nbsp;Yanying Wang ,&nbsp;Chunya Li ,&nbsp;Jinmiao Qu ,&nbsp;Dong Sun","doi":"10.1016/j.talanta.2025.127622","DOIUrl":"10.1016/j.talanta.2025.127622","url":null,"abstract":"<div><div>Hepatocellular carcinoma (HCC) stands as a grave illness characterized by elevated death rates. Early identification plays a vital role in improving patient survival. Herein, a novel split-type dual-mode biosensor featuring with near-infrared photoelectronchemical (PEC) and colorimetric sensing characteristics was developed for the high-performance detection of HepG2 cells. Biotin labeled aptamer (Bio-Apt1) was immobilized onto 96-well plates functionalized with streptavidin to capture HepG2 cells through specific binding. HepG2 cells were then labeled with another aptamer (Apt-2) by recognizing GPC3 on the surface of HepG2 cells. Apt 2 could form DNA double strand (dsDNA-ALP) with ALP-labeled complementary DNA (cDNA-ALP). Subsequently, ALP was released to catalyze AAP to form ascorbic acid (AA), and AA reduced HAuCl₄ to form gold nanoparticles (AuNPs). Then the mixture containing AuNPs was introduced onto the surface of Y-MOFs/GCE to enhance the photocurrent response. The change of photocurrent corresponding to the concentration of HepG2 cells can be used for the PEC determination. ALP can catalyze the hydrolysis of disodium phenyl phosphate to produce phenol, followed by a reaction with 4-aminoantipyrine and potassium ferricyanide, resulting in a quinone derivative for the colorimetric determination. The photoelectrochemical and colorimetric detection models show excellent selectivity and sensitivity in identifying HepG2 cells, exhibiting a linear reaction range from 1.0 × 10² to 1.0 × 10⁶ cells mL⁻¹ and a detection limit of 13 cells mL⁻¹ and 51 cells mL⁻¹, respectively. The dual-mode split type biosensor avoided direct damage to biomolecules from high-energy light, and the independent signal transduction enabled the acquisition of reliable results.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127622"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zirconium-doped iron oxide nanoparticles for enhanced peroxidase-like activity","authors":"Peng Hu,&nbsp;Mengxiang Li,&nbsp;Su Li,&nbsp;Shengqiang Wang","doi":"10.1016/j.talanta.2025.127629","DOIUrl":"10.1016/j.talanta.2025.127629","url":null,"abstract":"<div><div>Fe₃O₄ nanoparticles (NPs) have emerged as pioneering nanozymes with applications in clinical diagnosis, environmental protection and biosensing. However, it is currently limited by insufficient catalytic activity due to poor electron transfer. In this study, we synthesized electron-rich-Zr-doped defect-rich Fe₃O₄ NPs (Zr₃Fe₃O₄) using a one-pot solvothermal method. Compared with intrinsic Fe₃O₄ NPs, the resultant Zr₃Fe₃O₄ NPs exhibit enhanced peroxidase (POD)-like activity attributed to the presence of active centers of Zr-O-Fe bridges and adsorption sites of asymmetric oxygen vacancies (OVs) Zr-OVs-Fe. The Zr-O-Fe bridges facilitate electron transfer from Zr to Fe, promoting the regeneration of surface Fe²⁺ in Fe₃O₄ NPs. Furthermore, the rich Zr-OVs-Fe significantly enhances the adsorption and electron transfer between catalyst and substrates, thereby regulating the generation pathway of ¹O₂. Leveraging the remarkable POD-like activity of Zr₃Fe₃O₄ NPs, we developed a tandem enzyme-catalyzed reaction for colorimetric detection of glucose. This strategy of constructing active centers by atom doping provides valuable guidance for the development of more efficient Fenton-like catalytic systems with broad applications on a large scale.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"Article 127629"},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}