Talanta最新文献_第10页

Azo-PMA nanopores of sub-20 nm length for unimolecular resolution of nucleic acids and proteins. 亚20 nm长度的偶氮聚甲基丙烯酸甲酯纳米孔用于核酸和蛋白质的单分子分辨率。

IF 5.6 1区化学

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-16 DOI: 10.1016/j.talanta.2024.127402

Guodong Qi, Xun Chen, Daixin Liu, Meili Ren, Rong Tian, Liyuan Liang

{"title":"Azo-PMA nanopores of sub-20 nm length for unimolecular resolution of nucleic acids and proteins.","authors":"Guodong Qi, Xun Chen, Daixin Liu, Meili Ren, Rong Tian, Liyuan Liang","doi":"10.1016/j.talanta.2024.127402","DOIUrl":"10.1016/j.talanta.2024.127402","url":null,"abstract":"<p><p>Owing to the facile fabrication and surface modification, the cost-effective polymer nanopores are widely employed in unimolecular determination of biomacromolecules and selective sensing of small molecules, nanoparticles and biomarkers. However, the documented polymer nanochannels are generally microscale in length with low spatial resolution. We herein synthesized azobenzene side-chain polymer (Azo-PMA) and spin-coated on silicon nitride membrane to obtain a polymer film of nanoscale thickness for further nanopore generation via controlled dielectric breakdown (CDB) approach. The Azo-PMA nanopores demonstrate good ions transporting activities, pH tolerance and stability in high concentration of electrolyte with low ionic current noise. In addition, the azobenzene-containing polymer nanopores exhibit photo-response upon UV/Vis. light irradiation. The Azo-PMA nanopore devices are utilized for linear and quadruple nucleic acids discrimination, sensing of proteins with distinct shapes and sizes, as well as the single amino acid resolution with good capture rate and sensitivity. We established an unimolecular sensing platform using polymer nanopores for nucleic acids and proteins detection with good spatial resolution, which will be an addition for the nanopore-carrier material exploration and applications in potential genomics and proteomics with high spatiotemporal resolution and low cost.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127402"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-enrichment-free electrochemical detection of lead ions using functionalized tungsten oxide: Integration of surface functionalization and redox cycling mechanisms

IF 5.6 1区化学

Talanta Pub Date : 2025-03-11 DOI: 10.1016/j.talanta.2025.127923

Jie Gao , Yuanyuan Cui , Jun Yin , Xiangyue Wang , Xiaofeng Wei , Jingwen Zhang , Dong He , Hui Suo , Lan Ding , Chun Zhao

{"title":"Pre-enrichment-free electrochemical detection of lead ions using functionalized tungsten oxide: Integration of surface functionalization and redox cycling mechanisms","authors":"Jie Gao , Yuanyuan Cui , Jun Yin , Xiangyue Wang , Xiaofeng Wei , Jingwen Zhang , Dong He , Hui Suo , Lan Ding , Chun Zhao","doi":"10.1016/j.talanta.2025.127923","DOIUrl":"10.1016/j.talanta.2025.127923","url":null,"abstract":"<div><div>Methods for electrochemical detection of heavy metal ions have garnered widespread attention due to their high sensitivity, ease of operation, low cost, and suitability for on-site detection. However, these methods typically require a pre-enrichment step to improve the detection limit and sensitivity, which increases operational complexity and introduces potential errors. In this study, tungsten oxide electrodes with various functional groups were prepared by electrodeposition and high-temperature annealing, utilizing the amphoteric properties of <span>l</span>-alanine. The proposed electrodes exhibited high-performance Pb<sup>2+</sup> sensing, with a sensitivity of 26.20 μA μM<sup>−1</sup> cm<sup>−2</sup>, without pre-enrichment. Experimental results demonstrated that the electrode not only possesses excellent anti-interference capability and good reproducibility but also exhibits high accuracy and recovery rates in detecting actual water samples. This pioneering detection strategy harnesses tungsten's valence properties and surface functional groups' adsorption capacity to detect Pb<sup>2+</sup> directly. The method is simple, rapid, and efficient, making it suitable for routine monitoring of heavy metal ions in complex environments. This innovative approach holds significant promise for a wide range of applications in environmental monitoring.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"Article 127923"},"PeriodicalIF":5.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological advances and innovations in conjugated polymer films for high-performance gas sensors

IF 5.6 1区化学

Talanta Pub Date : 2025-03-11 DOI: 10.1016/j.talanta.2025.127904

Vinh Van Tran , Viet-Duc Phung , Ha Huu Do

{"title":"Morphological advances and innovations in conjugated polymer films for high-performance gas sensors","authors":"Vinh Van Tran , Viet-Duc Phung , Ha Huu Do","doi":"10.1016/j.talanta.2025.127904","DOIUrl":"10.1016/j.talanta.2025.127904","url":null,"abstract":"<div><div>Conjugated polymers (CPs) are considered one of the most important gas-sensing materials due to their unique features, combining the benefits of both metals and semiconductors, along with their outstanding mechanical properties and excellent processability. However, CPs with conventional morphological structures, such as largely amorphous and bulky matrices, face limitations in practical applications because of their inferior charge transport characteristics, low surface area, and insufficient sensitivity. Therefore, the design and development of novel morphological nanostructures in CPs have attracted significant attention as a promising strategy for improving morphological and electrical characteristics, thereby enabling a considerable increase in the sensing performance of corresponding gas sensors. Numerous CP nanostructures have been developed and implemented for high-performance gas sensors. Highlighting the morphological advances and bottlenecks of these nanostructures is crucial for providing an overview of developing trends, potential strategies, and emerging areas for the future development of CP nanostructures in the field. In this regard, this study describes state-of-the-art CP nanostructures, emphasizing their attractive morphological and electrical characteristics to help readers and researchers better understand emerging trends, promising future directions, and key obstacles for the application of CP nanostructure-based gas sensors. The most crucial aspects of CP nanostructures, including advanced preparation techniques, morphological properties, and sensing characteristics, are discussed and assessed in detail. Moreover, development strategies and perspectives for achieving high sensing efficiency in CP nanostructure-based flexible and wearable sensors are summarized and emphasized.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"Article 127904"},"PeriodicalIF":5.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A PEG-based strategy to improve detection of clinical microRNA 155 by bio-Field Effect Transistor in high ionic strength environment

IF 5.6 1区化学

Talanta Pub Date : 2025-03-11 DOI: 10.1016/j.talanta.2025.127881

Francesco Lavecchia di Tocco , Salvatore Cannistraro , Anna Rita Bizzarri

{"title":"A PEG-based strategy to improve detection of clinical microRNA 155 by bio-Field Effect Transistor in high ionic strength environment","authors":"Francesco Lavecchia di Tocco , Salvatore Cannistraro , Anna Rita Bizzarri","doi":"10.1016/j.talanta.2025.127881","DOIUrl":"10.1016/j.talanta.2025.127881","url":null,"abstract":"<div><div>microRNAs are small oligonucleotides involved in post-transcriptional gene regulation whose alteration is found in several diseases, including cancer, and therefore their detection is crucial for diagnosis, prognosis, and treatment purposes. Field-Effect Transistor-based biosensors (bioFETs) represent a promising technology for the clinical detection of microRNAs. However, one of the main challenges associated with this technology is the Debye screening, becoming significant at the high ionic strengths required for effective hybridization. We aimed at detecting oncogenic microRNA-155 by using a bioFET system using as capture element a complementary RNA probe (antimiR-155) combined with the introduction of PEG molecules (20 kDa, PEG20), at an ionic strength of 300 mM. We optimized the co-immobilization ratio between antimiR-155 and PEG20 and assessed its impact on the interactions between the oligonucleotides. The kinetics can be well described by the Langmuir-Freundlich isotherm with an affinity constant within the range typical of nucleic acid interactions. The introduction of PEG20 significantly enhanced the detection sensitivity of miR-155 by reaching a level of less than 200 pM, together with excellent discrimination against other clinically relevant microRNAs. Our findings demonstrate that the incorporation of PEG20 constitutes an effective strategy to mitigate the Debye screening effects and facilitates bioFET-based clinical applications at physiological ionic strengths.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"Article 127881"},"PeriodicalIF":5.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isotope-coded hydrazide tags for MALDI-MS based quantitative glycomics

IF 5.6 1区化学

Talanta Pub Date : 2025-03-10 DOI: 10.1016/j.talanta.2025.127921

Jian Fei , Si Liu , Qiuyue Sha , Chao Di , Zhaoyu Hu , Wei Du , Xin Liu

{"title":"Isotope-coded hydrazide tags for MALDI-MS based quantitative glycomics","authors":"Jian Fei , Si Liu , Qiuyue Sha , Chao Di , Zhaoyu Hu , Wei Du , Xin Liu","doi":"10.1016/j.talanta.2025.127921","DOIUrl":"10.1016/j.talanta.2025.127921","url":null,"abstract":"<div><div>The detection of glycosylation alterations is essential for elucidating the roles of glycan functions in biological processes and identifying potential disease biomarkers. Stable isotopic chemical labeling, coupled with mass spectrometry (MS), represents a powerful approach in quantitative glycomics. In this study, we synthesized a novel isotopic hydrazide pair, 2,6-Dimethyl-4-chinolincarbohydrazid (DMQCH) and its deuterium isomer DMQCH-<em>d</em><sub><em>4</em></sub>, via an efficient and cost-effective method, and applied it for the first time in MALDI-MS-based quantitative glycomics. The hydrazide tags, DMQCH/DMQCH-<em>d</em><sub><em>4</em></sub>, enabled stable mass shifts through reductive-terminal reactions with glycans, allowing for differential mass tagging of two samples without additional purification after derivatization. This DMQCH/DMQCH-<em>d</em><sub><em>4</em></sub> pair exhibited high derivatization efficiency (including on-target derivatization), substantial improvements in MS signal intensity (a 15-fold increase for maltoheptaose, high reproducibility (CV < 13.6 %), and excellent linearity (R<sup>2</sup> > 0.99) over two orders of magnitude in dynamic range for the relative quantitative analysis of maltoheptaose. Furthermore, this isotopic hydrazide pair was validated by successfully measuring changes in serum <em>N-</em>glycan profiles from individuals with healthy human serum control and ovarian cancer, highlighting its potential in quantitative glycomics for clinical applications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"Article 127921"},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A lipid droplet-targeted probe for imaging of lipid metabolism disorders during mitochondrial myopathy

IF 5.6 1区化学

Talanta Pub Date : 2025-03-10 DOI: 10.1016/j.talanta.2025.127901

Qiaowen Lin , Yixin Shi , Yumeng Wang , Shixian Cao , Yan Lin , Zhiqiang Liu , Xiaoqiang Yu , Kang-Nan Wang

{"title":"A lipid droplet-targeted probe for imaging of lipid metabolism disorders during mitochondrial myopathy","authors":"Qiaowen Lin , Yixin Shi , Yumeng Wang , Shixian Cao , Yan Lin , Zhiqiang Liu , Xiaoqiang Yu , Kang-Nan Wang","doi":"10.1016/j.talanta.2025.127901","DOIUrl":"10.1016/j.talanta.2025.127901","url":null,"abstract":"<div><div>Lipid metabolism is closely related to various biological processes in cells. The accumulation of Lipid droplets (LDs) is a typical manifestation of certain metabolic diseases, such as mitochondrial myopathy, which shows a significant increase in LDs. The accumulation of LDs can exacerbate the progression of disease, and lysosomes selectively degrade LDs to cope with this phenomenon. Visualizing lipid metabolism disorders and the interaction between LDs and other organelles is of great significance for the diagnosis and understanding of various physiological processes within cells in diseases. In this work, we synthesized two novel LD fluorescent probes and screened the best PDM, which exhibited stable fluorescence performance and strong photobleaching resistance in complex environments. The dynamics of intracellular LDs were tracked using PDM, and abnormal lipid metabolism within mitochondrial myopathy cells was visualized. This provides new tools and perspectives for studying LD dynamics and diagnosing mitochondrial myopathy.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"Article 127901"},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multifunctional biosensor for linked monitoring of inflammation indicators in hypertension drug evaluation and companion diagnostics

IF 5.6 1区化学

Talanta Pub Date : 2025-03-08 DOI: 10.1016/j.talanta.2025.127882

Jun Jiao , Lina Lu , Mengai Yin , Tong Wang , Zhijie Wang , Yu Zhu , Jie Zhao , Xiangrui Wang , Bingxin Hu , Fuju Dai , Lan Xue , Jiaxuan Qiao , Yue Liu , Qiang Chen

{"title":"A multifunctional biosensor for linked monitoring of inflammation indicators in hypertension drug evaluation and companion diagnostics","authors":"Jun Jiao , Lina Lu , Mengai Yin , Tong Wang , Zhijie Wang , Yu Zhu , Jie Zhao , Xiangrui Wang , Bingxin Hu , Fuju Dai , Lan Xue , Jiaxuan Qiao , Yue Liu , Qiang Chen","doi":"10.1016/j.talanta.2025.127882","DOIUrl":"10.1016/j.talanta.2025.127882","url":null,"abstract":"<div><div>Hypertension, often called the \"silent killer\", is a prevalent chronic disease closely linked to inflammation. However, most current methods monitor only single indicator, providing a limited view of inflammation in hypertension progression. To address this, we developed a multifunctional biosensor featuring a dual target linked monitoring (DTLM) Probe for the simultaneous detection of IL-6 and CRP, two key inflammatory markers in hypertension progression. The DTLM Probe, based on NH<sub>2</sub>-UiO-66@AuNPs with mutually non-interfering signal chains, was optimized for high performance in tracking both indicators simultaneously. The dual outputs operate independently, enabling IL-6 and CRP to be detected together or individually within a single sample injection. Under optimized conditions, the biosensor demonstrated excellent specificity and sensitivity, with detection limits of 355 fg/mL for IL-6 and 367 fg/mL for CRP. Applied to a rat model, the biosensor effectively explored the anti-inflammatory effects of Qishenyiqi, a traditional Chinese medicine, assessing its efficacy in reducing hypertensive heart damage. Additionally, it distinguished IL-6 and CRP levels between healthy and hypertensive individuals, capturing subtle changes after treatments. This ensured targeted anti-inflammatory therapies for patients who would benefit most. This biosensor provides a powerful and versatile platform for dual markers tracking, supporting both drug evaluation and companion diagnostics for tailor treatments in hypertension management.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"291 ","pages":"Article 127882"},"PeriodicalIF":5.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limonene monitoring in citrus industry wastewater using molecularly imprinted voltammetric sensor

IF 5.6 1区化学

Talanta Pub Date : 2025-03-08 DOI: 10.1016/j.talanta.2025.127831

Aléxia Massinatore Zancan , José Luiz da Silva , Nelson Ramos Stradiotto

{"title":"Limonene monitoring in citrus industry wastewater using molecularly imprinted voltammetric sensor","authors":"Aléxia Massinatore Zancan , José Luiz da Silva , Nelson Ramos Stradiotto","doi":"10.1016/j.talanta.2025.127831","DOIUrl":"10.1016/j.talanta.2025.127831","url":null,"abstract":"<div><div>The present work reports the development of a novel molecularly imprinted polymer (MIP) electrochemical sensor for limonene determination in orange industry wastewater. MIP sensor was constructed through the pyrrole electropolymerization in the presence of limonene on glassy carbon electrode surface (GCE/MIP). Electrode surface as characterized by electrochemical, spectroscopy and microscopic techniques. The analysis was performed using differential pulse voltammetry, employing hexacyanoferrate as the electrochemically active probe. Under optimized conditions, the proposed GCE/MIP sensor displayed linear range from 1.0 to 100 pmol L<sup>−1</sup> with a high sensitivity (0.92 nA L pmol<sup>−1</sup>) and low detection limit (0,87 pmol L<sup>−1</sup>), as well as excellent storage stability, repeatability and reproducibility. The imprinted factor found for the sensor was 4.1 with high selectivity. The applicability of the sensor was successfully evaluated by limonene determination in yellow water sample. GCE/MIP showed recovery from 97 to 105 %. The results, altogether, indicate that the GCE/MIP sensor can provide a sensitive and selective method for limonene determination with accuracy and precision.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"Article 127831"},"PeriodicalIF":5.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex lateral flow immunoassay for simultaneous detection and specific discrimination of Xoo and Xoc in rice plants

IF 5.6 1区化学

Talanta Pub Date : 2025-03-08 DOI: 10.1016/j.talanta.2025.127917

Cui Zhang , Xi Zhang , Nairu Liu , Jie Dong , Weijia Mao , Zhaoli Liu , Xueping Zhou , Jianxiang Wu

{"title":"Multiplex lateral flow immunoassay for simultaneous detection and specific discrimination of Xoo and Xoc in rice plants","authors":"Cui Zhang , Xi Zhang , Nairu Liu , Jie Dong , Weijia Mao , Zhaoli Liu , Xueping Zhou , Jianxiang Wu","doi":"10.1016/j.talanta.2025.127917","DOIUrl":"10.1016/j.talanta.2025.127917","url":null,"abstract":"<div><div><em>Xanthomonas oryzae</em> pv. <em>oryzae</em> (<em>Xoo</em>) and <em>Xanthomonas oryzae</em> pv. <em>oryzicola</em> (<em>Xoc</em>) are two important rice bacterial pathogens, causing serious losses of global rice yield every year. Effective management of rice bacterial diseases needs timely and accurate detection and quarantine techniques<em>.</em> Herein, a novel Au nanoparticles-based multiplex lateral flow immunoassay (AuNP-mLFIA) was developed for simultaneous detection and specific discrimination of <em>Xoo</em> and <em>Xoc</em> in rice leaf and seed samples. Four anti-<em>Xoo</em> mAbs, four anti-<em>Xoc</em> mAbs, and ten broad-spectrum mAbs towards both <em>Xoo</em> and <em>Xoc</em> were first obtained via hybridoma cell technique. Through checkerboard assays, pairwise mAbs 12H1/19C9 against <em>Xoo</em> and self-paired mAb 18D12 against <em>Xoc</em> were selected to guarantee the highest sensitivity and specificity of the assay. Under the optimal conditions, the AuNP-mLFIA achieved a visual limit of detection (LOD) as low as 7.8 × 10<sup>3</sup> CFU mL<sup>−1</sup> for <em>Xoo</em> and 1.6 × 10<sup>4</sup> CFU mL<sup>−1</sup> for <em>Xoc</em> with no cross-reactivity. In addition, the assay was successfully applied to monitor <em>Xoo</em> and <em>Xoc</em> in 40 field-collected rice leaf and seed samples and the detection results of this assay agreed well with conventional PCR. Therefore, this developed AuNP-mLFIA was sensitive, rapid, and accurate for simultaneous detection of <em>Xoo</em> and <em>Xoc</em>. This study also provides guidelines for the screening of paired mAbs to establish a double-antibody sandwich (DAS)-mLFIA.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"Article 127917"},"PeriodicalIF":5.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cysteine-assisted synthesis of copper nanoclusters for construction of FRET-based ratiometric sensor for visual detection of alkaline phosphatase

IF 5.6 1区化学

Talanta Pub Date : 2025-03-07 DOI: 10.1016/j.talanta.2025.127910

Qinhui Dong, Wei Song, Shusheng Zhang, Xiangfang Lin, Pengfei Shi

{"title":"Cysteine-assisted synthesis of copper nanoclusters for construction of FRET-based ratiometric sensor for visual detection of alkaline phosphatase","authors":"Qinhui Dong, Wei Song, Shusheng Zhang, Xiangfang Lin, Pengfei Shi","doi":"10.1016/j.talanta.2025.127910","DOIUrl":"10.1016/j.talanta.2025.127910","url":null,"abstract":"<div><div>Abnormal alkaline phosphatase (ALP) levels in the human body are closely associated with various diseases, particularly hepatobiliary diseases and bone diseases. Herein, we constructed a ratiometric sensor based on Förster resonance energy transfer (FRET) using strongly photoluminescent copper nanoclusters (Cu NCs) for the detection of ALP with high sensitivity and specificity. The cysteine-stabilized Cu NCs (Cys-Cu NCs) were synthesized through a ligand-exchange reaction and core-size etching focusing, which displayed bright photoluminescence (PL) with a quantum yield (QY) of 10.5 %. Multispectral characterization indicated that zwitterionic cysteine ligands without obvious steric hindrance could significantly enhance intra/inter-ligand-involved charge transfer, leading to a significant increase in fluorescence emission (∼14 folds) compared to precursor Cu NCs. A FRET-based ratiometric sensor for ALP detection was constructed by combining Cys-Cu NCs with a Cu<sup>2+</sup>-assisted oxidation reaction of <em>o</em>-phenylenediamine (OPD) to generate fluorescent 2,3-diaminophenazine (DAP). The strong coordination interaction between the ALP substrate and Cu<sup>2+</sup> significantly affected the FRET process between Cys-Cu NCs and DAP, thereby altering the fluorescence ratio. Based on the specific response of ALP to its substrate, the ratiometric sensors showed good linear relationship within the range of 0.1–50 U/L, with a detection limit (LOD) of 0.075 U/L. Furthermore, the FRET-based ratiometric sensor was integrated with a polymer hydrogel to fabricate a portable hydrogel sensor for simple and visual detection of ALP.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"291 ","pages":"Article 127910"},"PeriodicalIF":5.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0