Talanta最新文献

{"title":"A ratiometric fluorescence sensor based on integrated bimetallic Zr/Eu-MOF for rapid and visual detection of glyphosate.","authors":"Haojie Shi, Wei Wang, Haoyu Shi, Liangang Mao, Lan Zhang, Lizhen Zhu, Chi Wu, Xingang Liu","doi":"10.1016/j.talanta.2026.130308","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130308","url":null,"abstract":"<p><p>The widespread use of glyphosate in agriculture has raised concerns about environmental contamination and associated health risks, creating an urgent need for rapid, sensitive, and field-deployable analytical tools. In this work, Zr/Eu-metal-organic framework (Zr/Eu-MOFs) as an integrated bimetallic ratiometric fluorescent hydrogel sensor for fast and highly sensitive visual detection of glyphosate has been developed. By doping Eu³⁺ into a robust Zr-based MOF framework, Zr/Eu-MOFs exhibit dual-emission bands at 445 and 618 nm, enabling an intrinsic self-referenced signal without multicomponent assembly. Upon glyphosate addition, the sensor responds within 10 s. Zr/Eu-MOFs display a reliable ratiometric response over 0.05 - 10.0 mg/L with a low detection limit of 0.03 mg/L (0.2 μmol/L), which is far below the permissible maximum residue limit (MRL) for drinking water by the US EPA and China (4.1 μmol/L). Mechanistic evidence indicates that glyphosate forms P-O-Zr coordination linkages and interfacial interactions which modulate the ligand-to-metal charge transfer (LMCT) process, resulting in an enhanced ratiometric fluorescence response at 445 nm, while the Eu³⁺ emission at 618 nm remains essentially unchanged. Moreover, an agarose hydrogel format coupled with smartphone image analysis enables rapid visual detection. A spraying-enabled fluorescence imaging strategy further allows rapid screening of glyphosate contamination on leaves, apple surfaces, and soil-water matrices under UV illumination. Overall, this work provides a facile, integrated, and visually readable ratiometric platform for on-site glyphosate monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130308"},"PeriodicalIF":6.7,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148454219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral plasmonic Cu_2-xS quantum dots enable chirality-dependent fluorescent recognition of tryptophan enantiomers.","authors":"Meihan Guo, Ruiju Yang, Chenlei Gu, Lu Liu, Jian Wang, Shu Jun Zhen, Hong Yan Zou, Cheng Zhi Huang","doi":"10.1016/j.talanta.2026.130307","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130307","url":null,"abstract":"<p><p>The accurate discrimination and quantification of chiral amino acids enantiomers have remained significant challenges in both chemistry and biology. In this study, we employed an etching method to synthesize two types of chiral plasmonic fluorescent Cu_2-xS quantum dots for efficient enantioselective L/D-tryptophan (Trp) enantiomers. Specifically, homochiral interactions between the Cu_2-xS and Trp enantiomers led to fluorescence enhancement, which could be attributed to the aggregation-induced emission (AIE) effect. In contrast, heterochiral interaction led to negligible fluorescence change. Leveraging these fluorescence variations, sensitive discrimination and detection of Trp enantiomers using L-Cu_2-xS with a limit of detection (LOD) of 0.3 μM for L-Trp, where the enantioselective fluorescence enhancement ratio reached up to 447. And the LOD was 0.1 μM for D-Trp using D-Cu_2-xS, where the enantioselective fluorescence enhancement ratio was 11.8. This research not only provides an effective strategy for the preparation of chiral plasmonic materials with strong emission properties but also offers a simple yet sensitive method for chiral recognition, thereby opening up new perspectives and opportunities in related research fields.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130307"},"PeriodicalIF":6.7,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148443626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-function antifouling/sterilization electrically neutral hydrogel mediated bipolar autoluminescencet coreactant-free electrochemiluminescent biosensor.","authors":"Luting Yan, Lei Tian, Zekun Ren, Chao Wang, Xia Sun, Yemin Guo, Maja Milojević-Rakić, Yanyan Zhang","doi":"10.1016/j.talanta.2026.130301","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130301","url":null,"abstract":"<p><p>The scarcity of cathodic autoluminescencet restricts the development of coreactant-free electrochemiluminescent sensor for simultaneous detection of two targets. Herein, a novel coreactant-free electrochemiluminescent sensor was prepared based on Zr-DPA@TCPP MOF and AuAgNCs bimetallic nanoclusters served as a cathodic and anodic autoluminescencet emitter respectively. Significantly, this sensor system without any coreactants, effectively overcoming the simultaneous detection issue typically relying on different coreactants for cathode and anode luminescence. Meanwhile, complex matrix components can compromise the specificity and accuracy of the detection. Therefore, highly conductive and electrically neutral hydrogel was obtained to reduce interference from non-target substances. Poly (3,4-ethylenedioxythiophene): polystyrene sulphonate (PEDOT: PSS) was encapsulated in hydrogel to obtain PEDOT: PSS@hydrogel, besides, the crosslinking agents citronellal and TCPP provide a sterilization effect. Dual-function antifouling/sterilization electrically neutral hydrogel effectively addresses the main issues of complex matrix interference and secondary contamination. Under optimal conditions, the linear range was 1 × 10^-3 ∼ 1 × 10⁵ ng/L for Cd(II) and 1 ∼ 1 × 10⁸ CFU/mL for S. aureus. The biosensor achieves remarkably low limits of detection (LODs) at 1.71 pg/L and 1.36 CFU/mL for Cd(II) and S. aureus, respectively. This work offers a new perspective on the development of coreactant-free ECL technology with high accuracy and stability.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130301"},"PeriodicalIF":6.7,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synchronous modulation of fluorescence and oxidase-mimicking activity in a Mn-based MOF nanozyme via post-synthetic metal exchange for Cu²⁺ sensing.","authors":"Linying Cheng, Xiang Liu, Zixin Zhang, Jing Liu, Jianru Tang, Xiaohua Zhu, Youyu Zhang, Meiling Liu","doi":"10.1016/j.talanta.2026.130300","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130300","url":null,"abstract":"<p><p>Copper ions (Cu²⁺) are essential micronutrients but become hazardous when accumulated in excess, necessitating reliable, sensitive, and field-deployable detection methods. Herein, we report a fluorescent Mn-based MOF nanozyme (Mn-TCPP) that exhibits intrinsic oxidase-like activity, capable of catalyzing the oxidation of non-fluorescent o-phenylenediamine (OPD) to fluorescent 2,3-diaminophenazine (DAP) with an emission peak at 560 nm, while the Mn-TCPP itself displays characteristic fluorescence at 655 nm. Upon exposure to Cu²⁺, the stronger affinity of Cu²⁺ toward the porphyrinic TCPP ligand displaces Mn²⁺ from the framework, forming Cu-TCPP. This metal-exchange process simultaneously quenches the 655 nm fluorescence of the MOF and attenuates its oxidase-mimicking activity, whereas free Cu²⁺ in solution independently catalyzes OPD oxidation, further enhancing the 560 nm emission. Leveraging this dual-response mechanism, we develop a ratiometric fluorescence sensing strategy for Cu²⁺ detection based on the intensity ratio F₅₆₀/F₆₅₅. The calibration curve follows a linear equation: F₅₆₀/F₆₅₅ = 0.201x-0.001 (0.2-10 μM, R² = 0.985) and y = 0.002x+2.061 (10-100 μM, R² = 0.994) with the limit of detection (LOD) of 81 nM (S/N = 3). The sensor exhibits excellent selectivity over competing metal ions, and the method reliability is validated by standard addition recovery experiments in real water and rice samples. Furthermore, the Mn-TCPP nanozyme is embedded into an agarose hydrogel matrix, enabling visual, instrument-free monitoring of Cu²⁺ contamination: increasing Cu²⁺ concentrations induce a discernible fluorescence color transition from orange to yellow under 365 nm illumination. This work presents a tunable MOF platform that exploits competitive targets synchronous modulation of fluorescence and enzyme-mimicking activity, providing a robust, selective, and field-deployable ratiometric sensor for Cu²⁺.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130300"},"PeriodicalIF":6.7,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148454257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A gelatinase-responsive cascade microfluidic chemiluminescence assay for ultrasensitive detection of drug-resistant bacteria and antibiotic susceptibility.","authors":"Xueming Zhu, Wenchao Zhu, Bing Fan, Meng Gao, Dan Gao","doi":"10.1016/j.talanta.2026.130291","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130291","url":null,"abstract":"<p><p>Antibiotic-resistant bacterial infections pose a global health crisis, driving the need for innovative diagnostic approaches. Conventional methods for detecting resistant strains and antibiotic susceptibility testing (AST) are often limited by long assay times, low sensitivity, and operational complexity. Herein, a novel gelatinase-responsive cascade microfluidic chemiluminescence (CL) biosensor was developed for ultrasensitive detection of gelatinase-producing bacteria, with phenotypic AST validated using Staphylococcus aureus (S. aureus) as the model pathogen. Peptide-DNA probes were designed to respond to gelatinase secreted by bacteria remain metabolically active following antibiotic exposure. Bacterial gelatinase cleaves peptides to release initiator single-stranded DNA (ssDNA), triggering hybridization chain reaction (HCR) amplification in micropillar-structured microchannels. Through streptavidin-biotin interaction, poly-horseradish peroxidase (poly-HRP) is anchored in the channel to catalyze luminol based CL. This biosensor achieves high specificity and sensitivity, with a detection limit of 15 CFU/mL, which is better or comparable to previously reported biosensors. Moreover, the potential clinical applicability of this platform is further verified by testing four clinical S. aureus strains against five antibiotics, yielding 95% categorical agreement rate with standard AST methods. The area under the receiver operating characteristic ROC curve (AUC) was 0.944, close to the ideal value of 1, indicating excellent diagnostic performance for AST. The assay time from isolated colonies to AST result takes approximately 4-5 h. Featuring portability and low cost, the platform enables universal bacterial AST, providing a reliable tool for precise antimicrobial therapy and antimicrobial resistance control.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130291"},"PeriodicalIF":6.7,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymeric microneedle patches for rapid DNA extraction and molecular diagnosis of foliar pathogens of mung bean.","authors":"Asim Mushtaq, Barsha Poudel, Alina Karki, Pouria Azarikhah, Ahmed Saad, Adrian Dinsdale, Sonia Fiorito, Noel L Knight, Zahra Faraji Rad","doi":"10.1016/j.talanta.2026.130267","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130267","url":null,"abstract":"<p><p>Global food security is threatened by crop losses due to plant diseases. Early detection of pathogens is essential for implementing effective management practices to mitigate the negative impacts of disease. On-site molecular diagnostic techniques have significantly advanced for rapid and accurate identification of pathogens, but DNA extraction remains a labour-intensive and time-consuming bottleneck. This study demonstrates the ability of polyvinyl alcohol/polyvinylpyrrolidone (PVA/PVP) microneedle patches to extract detectable amounts of bacterial and plant DNA from diseased leaves within 1 min. This included DNA of a gram-negative Pseudomonas savastanoi pv. phaseolicola (Psp) and a gram-positive Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) bacteria, as well as mung bean (Vigna radiata). These bacteria cause halo blight and tan spot of mung bean, respectively. The DNA extracted using microneedle patches was of adequate quality and quantity to identify pathogens using real-time quantitative and droplet digital PCR (ddPCR). For leaves expressing halo blight symptoms, Psp DNA was detected in all DNA templates from microneedle (n = 5) and conventional extractions (n = 5). Similarly, in a time course study, Psp inoculated samples at day 6 showed the same detection in templates collected with microneedles and conventionally extracted samples. Detection of Cff DNA was less frequent, suggesting an impact of the sampling or inoculation technique. This study supports ongoing development of microneedle-based extraction as a simple and cost-effective method that could be used to support diagnosis of plant diseases, facilitate rapid decision-making for disease management, enhance biosecurity responses and area-freedom datasets, and prevent crop losses.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130267"},"PeriodicalIF":6.7,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148454247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ce-doped ZnO upconversion nanoparticles for optical thermometry, antibacterial therapy, and cardiac biomarker detection.","authors":"Yuemei Li, Yongmei Li","doi":"10.1016/j.talanta.2026.130277","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130277","url":null,"abstract":"<p><p>Lanthanide-doped upconversion nanoparticles (UCNPs) due to their unique optical properties are promising for applications ranging from bioimaging to biosensing. However, their practical implementation remains challenging owing to relatively low luminescence efficiency. Herein, we synthesize a series of Ce-doped Yb/Eu/ZnO, Yb/Er/ZnO and Yb/Tm/ZnO UCNPs via a hydrothermal method. Ce ions incorporation promotes anisotropic growth, which increases surface roughness and modulates the local coordination environment. Electrochemical impedance spectroscopy and Tafel plot analyses reveal that Ce doping significantly enhances charge separation and transfer kinetics. Ce ion doping markedly increases the emission intensities of Eu³⁺, Er³⁺ and Tm³⁺, attributed to the formation of Yb³⁺ dimers and efficient energy transfer under 980 nm excitation. Benefiting from these improvements, the Ce-doped ZnO UCNPs exhibit three distinct bio-applications: (i) superior low-temperature optical thermometry with a maximum relative sensitivity of 4.81% K^-1 at 80 K; (ii) excellent antibacterial activity against S. aureus and E. coli (up to 82.16% and 87.64% inhibition at 8 mol% Ce); and (iii) successful employment as luminescent probes in a multiplex immunoassay for the cardiac markers BNP (detection range 10-100 pg/mL) and ST2 (5-25 ng/mL), with high specificity and sensitivity. These findings underscore the potential of Ce-doped ZnO UCNPs as multifunctional platforms for optical thermometry, antibacterial therapy and biomedical diagnostics.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130277"},"PeriodicalIF":6.7,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148443569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel targeted high-throughput workflow for serum chemical mixture profiling in population exposome-wide association studies.","authors":"Yan Wu, Linlin Chen, Jie Zhang, Lisong Chen, Shanshan Du, Rui Ding, Weimin Ye","doi":"10.1016/j.talanta.2026.130275","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130275","url":null,"abstract":"<p><p>Simultaneous determination of environmental pollutants with complex compositions and wide-ranging acid dissociation constants in biological matrices presents a significant analytical challenge. This study developed a highly sensitive and broad-spectrum analytical method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). By employing phospholipid removal purification (PRP) technology and optimizing mobile phase pH conditions, we achieved simultaneous quantification of 230 environmental pollutants in serum. Comparative evaluation of sample pretreatment techniques revealed that low-temperature concentration caused preservative loss, while liquid-liquid extraction showed poor recovery for multiple compound classes. The PRP method was selected as the optimal approach due to its superior performance in reducing matrix effects compared to direct protein precipitation. Method validation demonstrated accurate quantification of all target analytes (relative standard deviation, RSD <15.1%), with 98.3% of compounds exhibiting satisfactory recoveries (80-120%). Analysis of serum samples from the Fuqing cohort participants detected 22 contaminants with detection frequencies >30%, among which PFHpA and PFOA were identified as risk factors for hyperuricemia. Notably, we provide the first evidence of a dose-response relationship between dehydroacetic acid exposure and hyperuricemia prevalence in a human population. This study established a robust solution for simultaneous screening of exposome-scale pollutants in complex biological samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130275"},"PeriodicalIF":6.7,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autocatalytic DNA cascade circuits via split-triggered recombination for ultrasensitive programmable nucleic acid detection.","authors":"Shanshan Tao, Suqin Wang, Songbai Zhang, Hongbo Li, Ruqin Yu","doi":"10.1016/j.talanta.2026.130262","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130262","url":null,"abstract":"<p><p>Accurate detection of microRNAs is of great significance for the clinical diagnosis and therapeutic guidance of cancer. Herein, we have established an isothermal self-catalytic DNA circuit that can combine an autocatalytic hairpin assembly (ACHA) converter with autocatalytic hybridization chain reaction (AHCR) amplification for the sensitive detection of miRNA-21. HCR is initiated by the recognition of the target analyte, generating new trigger strands that subsequently activate the CHA amplifier. One terminus of the CHA amplifier is engineered to expose the identical HCR initiator sequence, thereby enabling sustained HCR propagation. The DNAzyme moiety at the opposite terminus functions as a fluorescent signal reporter, efficiently cleaving the fluorogenic reporter probe. Therefore, the self-catalytic feedback loop between the HCR and CHA systems allows trace targets to produce fluorescence signals with exponential amplification. This circuit conducted a sensitive, reliable and universal analysis of miRNA-21, with a detection limit of 33.4 fM. Self-feedback cascade circuits provide a promising tool for molecular sensing in complex biological environments, this cascaded circuit can be used for the analysis of miRNA-21 in human serum samples. It has broad application prospects in low-abundance biomolecular sensing and early diagnosis of diseases.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130262"},"PeriodicalIF":6.7,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148443542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical detection of cisplatin-DNA adduct formation using dielectrophoresis-based impedance spectroscopy.","authors":"Michihiko Nakano, Masafumi Inaba, Boonchai Techaumnat, Junya Suehiro","doi":"10.1016/j.talanta.2026.130268","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130268","url":null,"abstract":"<p><p>Chemicals that form DNA adducts can be carcinogenic; therefore, their detection is essential for cancer research and genotoxicity screening. However, existing methods often require complex instrumentation or electrode modification, highlighting the need for simpler detection approaches. We aimed to develop a simple and rapid method for detecting DNA adduct formation using dielectrophoresis (DEP)-based impedance spectroscopy. Cisplatin was used as a model DNA-adduct-forming agent and incubated with DNA-labeled microbeads. Changes in DEP behavior were analyzed through crossover frequency measurements and impedance spectroscopy during frequency sweeps (200 kHz-10 MHz). Cisplatin treatment under conditions intended to promote DNA adduct formation was associated with a concentration-dependent decrease in the crossover frequency of DNA-labeled microbeads. During frequency sweeps, the conductance changed in the frequency region corresponding to the DEP transition, and the peak frequency derived from the conductance response showed strong agreement with the crossover-frequency trend. Quantitative detection was achieved over 0.05-0.5 mM, with a detection limit of 0.05 mM (50 μM). This DEP-based impedance approach offers a simple, cost-effective alternative to conventional methods that require electrode modification. Although the detection limit requires substantial improvement for future biological or clinical applications, the method provides a simple proof-of-concept platform for preliminary screening and methodological development.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"311 ","pages":"130268"},"PeriodicalIF":6.7,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}