Talanta最新文献

{"title":"Real-time visualization of \"small Molecules-ssDNA\" complexes for aptamer screening based on online competition CE-SELEX","authors":"Ge Yang ,&nbsp;Wenzhe Tian ,&nbsp;Yangyang Hu ,&nbsp;Yalun Wen ,&nbsp;Yi Zhao ,&nbsp;Guangyu Jiang ,&nbsp;Hao Liu ,&nbsp;Liping Zhao ,&nbsp;Chao Zhu ,&nbsp;Feng Qu","doi":"10.1016/j.talanta.2024.127199","DOIUrl":"10.1016/j.talanta.2024.127199","url":null,"abstract":"<div><div>Capillary electrophoresis-based systematic evolution of ligands by exponential enrichment (CE-SELEX) is one of the most efficient techniques for aptamers screening. However, CE-SELEX is generally considered challenging for small molecule targets due to a slight charge-to-mass ratio (z/m) difference that fails to create a clear separation between small molecule-ssDNA complexes and ssDNA libraries, thus heavily restricting the screening target scopes. Herein, a novel online competition CE-SELEX (ocCE-SELEX) strategy was introduced for real-time visualization of \"small molecules-ssDNA\" complexes to screen aptamers for small molecule targets in free solution. To achieve this vision, the \"pair-wise\" interaction-based \"Catcher-ssDNA-Target\" ternary system was flexibly designed via online CE mode. First, we introduced a conceptual protein of an ssDNA catcher (Catcher) that is capable of capturing all sequences in ssDNA library completely through an online controllable procedure by adjusting the injection time of different zones, resulting in the disappearance of original peaks of the ssDNA library in electropherogram. Then, the \"pair-wise\" interaction would restore the peaks through an online competition process, in which the Catcher with a faster migration rate would traverse the equilibrium zone of small molecule/ssDNA mixture and capture free ssDNA, as well as compete with small molecules to capture weakly bound ssDNA sequences. Due to the different z/m changes, the ssDNA captured by Catcher continues to migrate rapidly and the retained ssDNA in small molecule-ssDNA complex approximately maintains its original migration rate. Consequently, the peaks of Catcher-captured ssDNA and small molecule-ssDNA complex achieved complete separation and real-time visualization, allowing the complex to be collected readily. Furthermore, the implementation of ocCE-SELEX strategy was verified with two small molecules (vitamin B12 and ofloxacin) and six polypeptides. The study expands the application of CE-SELEX in small molecules in terms of real-time visualization and accurate collection of small molecules-ssDNA complexes, opening a new path for aptamer screening for small molecule targets in their natural state.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"284 ","pages":"Article 127199"},"PeriodicalIF":5.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A magnetic dual-aptamer electrochemical sensor with MOF-on-MOF-derived electrocatalyst as a signal amplifier for sensitive detection of cardiac troponin I.","authors":"Xiaolun Peng, Ruijie Xu, Fan Yu, Junhui Xu, Yazhen Wang, Shengfu Wang","doi":"10.1016/j.talanta.2024.127177","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.127177","url":null,"abstract":"<p><p>Considering the close association between cardiac troponin I (cTnI) level and various cardiovascular diseases, it becomes essential to explore sensitive and accurate detection methods for monitoring their levels in the early stages of disease. In this work, a magnetic dual-aptamer electrochemical sensor for cTnI detection was constructed in the first utilizing MOF-on-MOF-derived electrocatalyst as a signal amplifier in collaboration with high-efficient separation of magnetic beads (MBs). Employing zeolitic imidazolate framework-67 (ZIF-67) with high surface area as host MOF, MOF-on-MOF heterostructure (ZIF-67@PBA) was facilely prepared by in-situ growth of conductive prussian blue analogue (PBA) as guest MOF onto the surface of ZIF-67 with a simple ion-exchange method. After low-temperature calcination, N doped derived electrocatalyst (N-ZIF-67@PBA) was obtained with intact skeletons and pore structures of MOFs. This not only integrated bimetallic active centers with various valence states and diversiform nanostructures of dual MOF, but endowed N-ZIF-67@PBA 8.3-fold increase of electrocatalytic activity for catalytic amplification. Further using aptamer-modified MBs as capture carriers for recognizing and separating cTnI from complex samples with high specificity, the magnetic dual-aptamer sensor successfully achieved the sensitive detection of cTnI with a low detection limit of 0.31 fg/mL. This work provided a new viewpoint on the use of MOF-on-MOF-derived electrocatalyst for ultrasensitive electrochemical sensing analysis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"283 ","pages":"127177"},"PeriodicalIF":5.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and synthesis of esterase-activated fluorescent probe for diagnosis and surgical guidance of liver cancer.","authors":"Yaling Su, Zhongsheng Xu, Jiemin Wang, Jing Qian, Cong Liu, Junqi Shi, Wei Liu, Xiaoli An, Wenwu Qin, Yun Liu","doi":"10.1016/j.talanta.2024.127210","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.127210","url":null,"abstract":"<p><p>Liver cancer seriously threatens the health of human beings. Studies have found that esterase is overexpressed in liver cancer cells. Therefore, esterase can be one of the biomarkers of liver cancer. Previous literature studies have shown that the structures of fluorescent probe detection groups significantly impact the probes themselves and enzyme detection. In this paper, three \"off-on\" esterase-activated fluorescent probes (RHO-1, RHO-2 and RHO-3) with different length of the carbon chains of the detection groups were designed and synthesized. Density functional theory (DFT) calculation and Michaelis-Menten equations were applied to study the optical properties and affinity with esterase of the probes. Compared with RHO-1 and RHO-2, RHO-3 showed superior optical properties and affinity with esterase. Subsequently, RHO-3 was further used to detect esterase activity in vitro and in vivo. RHO-3 was the first esterase-activated fluorescent probe applied to image-guided diagnosis and surgical resection of liver cancer. It was expected to be a promising molecular imaging diagnostic tool in clinical applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"283 ","pages":"127210"},"PeriodicalIF":5.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput screening of fentanyl analogs.","authors":"Samuel A Miller, Andrew R Forero, Lilian Valadares Tose, Jordan E Krechmer, Felician Muntean, Francisco Fernandez-Lima","doi":"10.1016/j.talanta.2024.127191","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.127191","url":null,"abstract":"<p><p>This study presents an analytical approach coupling novel ambient ionization sources with trapped ion mobility spectrometry (TIMS) and tandem mass spectrometry (MS/MS) for the rapid characterization of fentanyl analogs. Two ambient ionization sources were illustrated for minimal sample preparation and rapid analysis: electrospray ionization (nESI) and direct analysis in real time (DART). Fentanyl analogs can be separated using nESI-TIMS-MS/MS based on differences in their mobility and/or fragmentation pattern; reference mobility spectra are reported for 234 single standards. In contrast, DART-TIMS-MS/MS allowed for the characterization of 201 compounds due to differences in the protonation pattern and efficiency when compared to nESI. The TIMS high resolving power (R > 80) allowed baseline separation for most isomers and mobility trends were established for methylated and fluorinated isomers, with the more compact ortho-substituted analogs showing distinct separation from para- and meta-substituted species. This multi-dimensional strategy offers a comprehensive characterization of fentanyl analogs and other synthetic opioids with minimal sample preparation. This analysis shows significant potential for high-throughput screening (<5 min) and high sensitivity detection (<pg level) of emerging illicit drugs, supporting ongoing forensic investigations and public health initiatives. The use of alternative mobility calibration methods using internal/external standards with ambient ionization sources coupled to TIMS-MS is also provided, enhancing its robustness and applicability.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"283 ","pages":"127191"},"PeriodicalIF":5.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic study for the identification of symptomatic carotid plaque biomarkers","authors":"Marina Botello-Marabotto ,&nbsp;Emma Plana ,&nbsp;M. Carmen Martínez-Bisbal ,&nbsp;Pilar Medina ,&nbsp;Andrea Bernardos ,&nbsp;Ramón Martínez-Máñez ,&nbsp;Manuel Miralles","doi":"10.1016/j.talanta.2024.127211","DOIUrl":"10.1016/j.talanta.2024.127211","url":null,"abstract":"<div><div>Carotid artery stenosis is mainly produced due to the progressive accumulation of atherosclerotic plaque in the vascular wall. The atherosclerotic plaque is characterized by the accumulation of lipids, low density proteins, expression of chemokines and adhesion molecules, and migration of monocytes and lymphocytes into the plaque. Its rupture can produce stroke, but embolic propensity depends principally on the composition and vulnerability of plaque rather than the severity of stenosis. It is important, then, to ascertain which patients with carotid artery stenosis have a greater risk of developing neurological symptomatology. Here, we present a metabolomic study by using nuclear magnetic resonance (NMR) spectroscopy in atheroma plaque and serum samples from patients with recently symptomatic and asymptomatic carotid stenosis to search for metabolites that could be used as biomarkers associated with plaque vulnerability and subsequent risk of rupture. Thirty-eight atheromatous plaque samples (24 asymptomatic patients and 14 symptomatic) and 70 serum samples (43 asymptomatic and 27 symptomatic) were studied by NMR spectroscopy. The data were analysed using multivariate statistics (PLS-DA) to determine a model to discriminate between symptomatic and asymptomatic samples (atheroma plaques and sera). The calculated PLS-DA models showed a 100 % sensitivity and a 96.6 % specificity for the cross validation to discriminate between symptomatic and asymptomatic plaques, and 88.37 % sensitivity and 77.78 % specificity when serum samples were analysed. According to the results of our multivariate and univariate analysis, the most discriminative metabolites for plaque vulnerability were threonine in serum samples, and glutamate in plaque samples. Also, an analysis of the main metabolic pathways involved in plaque vulnerability revealed that <span>d</span>-glutamine and <span>d</span>-glutamate metabolism, and phenylalanine, tyrosine, and tryptophan biosynthesis were the most affected pathways in plaque and serum, respectively.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"284 ","pages":"Article 127211"},"PeriodicalIF":5.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Notice to \"Reverse iontophoresis generated by porous microneedles produces an electroosmotic flow for glucose determination\" [Talanta 267 (2024) 125156].","authors":"Qi-Yao He, Jia-Hao Zhao, Shi-Ming Du, De-Gui Li, Zhi-Wei Luo, Xue-Qiu You, Jing Liu","doi":"10.1016/j.talanta.2024.127102","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.127102","url":null,"abstract":"<p><p>This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/policies/article-withdrawal). This article has been retracted at the request of the Editor-in-Chief. The authors have plagiarized part of a paper that had already appeared in Nat. Commun.12, (2021) 658, https://doi.org/10.1038/s41467-021-20948-4. One of the conditions of submission of a paper for publication is that authors declare explicitly that their work is original and has not appeared in a publication elsewhere. Re-use of any data should be appropriately cited. As such this article represents a severe abuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"127102"},"PeriodicalIF":5.6,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary glucose detection based on platinum metal hydrogel prepared mouthguard electrochemical sensor.","authors":"Dingxi Lu, Haotian Li, Nan Xiao, Mengyi Jiang, Yeerkentai Zuna, Shiqin Feng, Zhanhong Li, Jianjun Long, Jean Louis Marty, Zhigang Zhu","doi":"10.1016/j.talanta.2024.127197","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.127197","url":null,"abstract":"<p><p>A mouthguard electrochemical sensor for salivary glucose detection based on platinum metal hydrogel is proposed in this work. Conventional enzyme-based electrochemical glucose sensors are fraught with issues such as high cost, oxygen dependency, intricate immobilization procedures, and susceptibility to variations in temperature, pH, and so on. The detection of glucose in saliva, as a non-invasive sensing approach, presents a more convenient solution for diabetes monitoring. This study employs Pt metal hydrogel as the electrocatalytic material for glucose, with its microstructure characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques. The sensor's electrochemical properties, sensing performance, anti-interference capability, and stability were assessed through methods including cyclic voltammetry (CV), chronoamperometry, and electrochemical impedance spectroscopy (EIS). Under neutral pH phosphate buffer (PB) solution in the laboratory setting, the sensor demonstrated an outstanding linear range (0-40 mM) and a low detection limit (0.119 mM). Implemented in a wearable mouthguard format, this electrochemical sensor enables the detection of glucose in physiological environments, specifically saliva, exhibiting favorable detection characteristics: a linear range of 0.58-3.08 mM and a detection limit of 0.082 mM. This innovation thus offers a practical and efficacious tool for the non-invasive monitoring of glucose levels relevant to diabetes management.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"283 ","pages":"127197"},"PeriodicalIF":5.6,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric polymerase chain reaction mediated by pH indicator: Rapid detection of drug-resistant nosocomial bacteria","authors":"Thi Ngoc Diep Trinh ,&nbsp;Kieu The Loan Trinh ,&nbsp;Nae Yoon Lee","doi":"10.1016/j.talanta.2024.127193","DOIUrl":"10.1016/j.talanta.2024.127193","url":null,"abstract":"<div><div>Polymerase chain reaction (PCR) is the gold standard for molecular diagnoses due to its high sensitivity and accuracy. However, conventional PCR exhibits limitations including extended analysis time and complicated sample treatment. To shorten the analysis time of standard three-step PCR, we employed a two-step PCR allowing for fast amplification of nucleic acid in a chip format. Moreover, to realize a fully-integrated and on-site visual discrimination of opportunistic and multidrug-resistant pathogens, namely, <em>Enterococcus faecium</em> and <em>Acinetobacter baumannii</em>. DNA was extracted using FTA card, and pH-dependent color changes of the PCR amplicons were monitored by using phenolphthalein as a pH indicator. The overall reaction took less than 35 min and exhibited high selectivity and sensitivity confirmed by the limit of detection of approximately 10³ CFU/mL for <em>E. faecium</em>. In addition, carbapenem-resistant <em>A. baumannii</em> was successfully detected demonstrating the clinical applicability of the introduced technique using a thermoplastic chip. <em>E. faecium</em> both in bacterial cell culture solution and a contaminated surface were also successfully detected proving the feasibility of the chip-based detection system and paving the way for a facile discrimination of opportunistic pathogens prevalent in hospitals and our environment.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"284 ","pages":"Article 127193"},"PeriodicalIF":5.6,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of fluorescent biosensor based on aptamer recognition and enzyme-free nucleic acid signal amplification reaction and its application in cocaine detection","authors":"Yuan Zhang,&nbsp;Quanfang Li,&nbsp;Chen Ma,&nbsp;Shuoyun Wei,&nbsp;Shufang Ren","doi":"10.1016/j.talanta.2024.127165","DOIUrl":"10.1016/j.talanta.2024.127165","url":null,"abstract":"<div><div>Cocaine is a white crystalline alkaloid extracted from coca leaf. It can block human nerve conduction, produce local anesthetic effect, and has strong addiction. Therefore, the qualitative and quantitative analysis of cocaine is of great significance in forensic toxicology, pharmacy and metabolomics. Cocaine aptamer probe is expected to become a powerful tool for on-site detection of cocaine due to its good stability, fast response speed, and easy preparation of kits for on-site application. In our work, we construct a fluorescent biosensor for the detection of cocaine by using the characteristics of specific binding of nucleic acid aptamers to targets and combining with enzyme-free nucleic acid signal amplification reaction. First, streptavidin-modified magnetic beads (MBs) can specifically bind to biotin-modified cocaine aptamer probe (Aptamer) to form MB-DNA. The nucleic acid aptamer complementary probe (tDNA) can hybridize with the aptamer through base complementary pairing to form a double strand, thereby obtaining the MB-DNA-tDNA complex. When cocaine is present in the system, cocaine specifically binds to aptamer, thereby replacing the probe tDNA. The probe tDNA can undergo hybridization chain reaction with the hairpin probe 1 (HP1) and hairpin probe 2 (HP2), resulting in the opening of the hairpin structure, and the fluorescence signal response of the fluorophore 6-carboxyfluorescein (FAM) modified on the HP1 is turned on, so as to achieve rapid, low-cost and efficient cocaine detection. The establishment of this method can enrich and develop the basic theory of drug analysis and detection, and provide theoretical guidance and technical support for the accurate, efficient and convenient detection of drug analysis methods on site.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"283 ","pages":"Article 127165"},"PeriodicalIF":5.6,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic enhancement of collagen detection using lanthanide-functionalized collagen targeted peptides.","authors":"Jean Claude Munyemana, Xiuxia Sun, Lu Li, Chunxia Zhang, Eskandar Qaed, Jianxi Xiao","doi":"10.1016/j.talanta.2024.127170","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.127170","url":null,"abstract":"<p><p>Monitoring collagen denaturation is crucial for diagnosing collagen-related diseases such as tumors and fibrosis. Herein, we have developed specific probes to detect denatured collagen (d-Col) and collagen I (Col I), utilizing peptide probes with sequences (GOP)₁₀ and HVWMQAP, targeting at d-Col and Col I, respectively. These peptides were conjugated with 1,10-phenanthroline-5-carboxylic Acid (Phen), forming Phen-Ahx-(GOP)₁₀ and Phen-Ahx-HVWMQAP. Phen acts as both an antenna sensitizer and a chelator, coordinating with Terbium (III) and Europium (III) ions via its nitrogen atom, facilitating fluorescent emission in green and red, respectively. The investigation demonstrated that Tb³⁺ interacts with three (GOP)₁₀ peptide units through Phen, while Eu³⁺ connects with four units of Ahx-HVWMQAP peptides. Additionally, it is important to note that the structure of the peptides remains unchanged after chelating with the lanthanide ions, maintaining their integrity throughout the process. These probes have effectively demonstrated their ability to bind to specific collagen types with selectivity, enabling accurate identification of their presence. The excellent binding of these probes is due to the branched structure of the formed lanthanide-peptide complexes. A dose-dependent linear association was observed in the binding of Eu-[Phen-Ahx-HVWMQAP]₄ to Col I, with concentration levels ranging from 0.5 to 100 μM and a minimal detectable concentration of 0.113 μM. We anticipate that our developed probes will improve understanding of collagen remodeling and provide opportunities for the diagnosis of collagen-associated diseases.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"283 ","pages":"127170"},"PeriodicalIF":5.6,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}