Talanta最新文献_第10页

Development of a novel method of biochemical oxygen demand colorimetric detection and its application in actual water 生化需氧量比色检测新方法的建立及其在实际水体中的应用

IF 5.6 1区化学

Talanta Pub Date : 2025-07-07 DOI: 10.1016/j.talanta.2025.128563

Ling Yang , Jihao Su , Zhixuan Yu , Yanqing Chang , Dengbin Yu , Zhiquan Zhang , Shaojun Dong

{"title":"Development of a novel method of biochemical oxygen demand colorimetric detection and its application in actual water","authors":"Ling Yang , Jihao Su , Zhixuan Yu , Yanqing Chang , Dengbin Yu , Zhiquan Zhang , Shaojun Dong","doi":"10.1016/j.talanta.2025.128563","DOIUrl":"10.1016/j.talanta.2025.128563","url":null,"abstract":"<div><div>In this study, a novel and efficient colorimetric biosensor was constructed for biochemical oxygen demand (BOD) detection based on visual analysis and Qualcomm measurement with enzyme-labeling instrument. Microorganisms cultured in situ with actual water and Prussian blue were used as model bacteria and indicator of colorimetric biosensor, respectively. To accurately detect BOD content in water, the reaction conditions such as cell concentration, K<sub>3</sub>[Fe(CN)<sub>6</sub>] concentration, reaction temperature, and reaction time were optimized. The optimal conditions were OD<sub>600</sub> = 7, 10 mM, 37 °C and 40 min, respectively. The dominant bacteria in the color reaction process were <em>Acinetobacter</em>, <em>Bacillus</em> and <em>Exiguobacterium</em>. The detection range of the biosensor was 5–80 mg L<sup>−1</sup>, and the detection limit was 3.32 mg L<sup>−1</sup>. The relative error of BOD content in actual water was 15.49 %. In summary, the high-efficiency colorimetric biosensor can detect BOD content quickly and sensitively, which implied its potential application prospects in the field of BOD detection.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128563"},"PeriodicalIF":5.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vanadium oxide compounds as mimic enzymes: hidden catalytic ability 钒氧化物化合物作为模拟酶：隐藏的催化能力

IF 5.6 1区化学

Talanta Pub Date : 2025-07-07 DOI: 10.1016/j.talanta.2025.128562

Jingtian Chi , Peng Ju , Zhenbo Xiang , Siyu Wen , Tiantong Jiang , Quantong Jiang , Xiaofan Zhai

{"title":"Vanadium oxide compounds as mimic enzymes: hidden catalytic ability","authors":"Jingtian Chi , Peng Ju , Zhenbo Xiang , Siyu Wen , Tiantong Jiang , Quantong Jiang , Xiaofan Zhai","doi":"10.1016/j.talanta.2025.128562","DOIUrl":"10.1016/j.talanta.2025.128562","url":null,"abstract":"<div><div>Nanozymes, innovative artificial enzymes crafted from engineered nanomaterials, have been designed to address the inherent limitations of natural enzymes, such as expensive storage requirements, lack of structural stability, and sensitivity to chemicals. Vanadium oxide-based compounds have garnered significant interest lately, owing to their excellent biocompatibility, potent catalytic performance, and ease of surface modification, positioning them as a promising candidate for use in biosensing and environmental remediation. In this review, vanadium oxide-based compounds that mimicked different oxidoreductases, exhibiting peroxidase-, catalase-, oxidase/laccase-, and superoxide dismutase-like activities were summarized. Moreover, the current review offers an in-depth examination of the mechanisms, latest progress, and practical applications associated with each reported oxidoreductase-like function of nanozymes constructed from vanadium oxide-based compounds. In brief, this review identifies and discusses the present hurdles in the transition of vanadium oxide-based compounds into viable applications, providing recommendations for future research avenues.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128562"},"PeriodicalIF":5.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applying pulse UV irradiation-induced chemiluminescence approach for high-throughput screening assay of tyrosinase inhibitors 应用脉冲紫外照射诱导化学发光法进行酪氨酸酶抑制剂高通量筛选试验

IF 5.6 1区化学

Talanta Pub Date : 2025-07-07 DOI: 10.1016/j.talanta.2025.128560

Ali Abdel-Hakim , Mahmoud El-Maghrabey , Ayaka Tsubokami , Fathalla Belal , Mohamed A. Hammad , Naotaka Kuroda , Naoya Kishikawa

{"title":"Applying pulse UV irradiation-induced chemiluminescence approach for high-throughput screening assay of tyrosinase inhibitors","authors":"Ali Abdel-Hakim , Mahmoud El-Maghrabey , Ayaka Tsubokami , Fathalla Belal , Mohamed A. Hammad , Naotaka Kuroda , Naoya Kishikawa","doi":"10.1016/j.talanta.2025.128560","DOIUrl":"10.1016/j.talanta.2025.128560","url":null,"abstract":"<div><div>Tyrosinase is an enzyme that metabolizes L-tyrosine and is found in various organisms. Its overactivity can lead to health issues in humans, such as hyperpigmentation, and can adversely affect human skin, leading to skin cancers. This has heightened the significance of tyrosinase inhibitors in pharmaceuticals and cosmetics, particularly skin-whitening formulations. In this study, we developed a high-throughput screening assay for identifying tyrosinase inhibitors. This assay leverages the strong chemiluminescence signal emitted by L-tyrosine upon nanosecond UV irradiation in the presence of L-012 chemiluminescence dye, which is based on the formation of reactive oxygen species (ROS). We measured the decrease in chemiluminescence signal induced by tyrosinase enzyme, which converts chemiluminescent L-tyrosine into non-chemiluminescent L-DOPA. The addition of tyrosinase inhibitors prevents this conversion, leading to recovery in chemiluminescence of L-tyrosine. However, the reliability of the assay can be compromised by the ROS-scavenging activity and phenolic nature of certain enzyme inhibitors. To mitigate potential false results caused by some inhibitors, tyrosinase was immobilized on the microplate surface, and the inhibitors were incubated with the fixed enzyme, then, the enzyme activity was assessed after washing away the inhibitors. The proposed assay successfully facilitated high-throughput screening (less than 1 min per sample) of numerous tyrosinase inhibitor candidates from various pharmacological classes. The percentage inhibition of tyrosinase activity determined by our assay was statistically compared with results from a previously reported assay, revealing comparable outcomes and confirming the reliability of our approach. In addition, we evaluated the environmental impact and applicability of the assay using two recent metrics, yielding promising results.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128560"},"PeriodicalIF":5.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility of laser-induced breakdown spectrometry for elemental mapping in fish-mint plant (Houttuynia cordata Thunb.) leaves exposed to iron 激光诱导击穿光谱法在鱼薄荷植物（鱼腥草cordata Thunb.）暴露于铁中的可行性

IF 5.6 1区化学

Talanta Pub Date : 2025-07-05 DOI: 10.1016/j.talanta.2025.128552

Leon de Oliveira Lima , Aline Pereira de Oliveira , Higor Bolignano de Oliveira , Cassiana Seimi Nomura , Juliana Naozuka

{"title":"Feasibility of laser-induced breakdown spectrometry for elemental mapping in fish-mint plant (Houttuynia cordata Thunb.) leaves exposed to iron","authors":"Leon de Oliveira Lima , Aline Pereira de Oliveira , Higor Bolignano de Oliveira , Cassiana Seimi Nomura , Juliana Naozuka","doi":"10.1016/j.talanta.2025.128552","DOIUrl":"10.1016/j.talanta.2025.128552","url":null,"abstract":"<div><div>Laser-induced breakdown spectroscopy (LIBS) emerges as a rapid, direct analysis method requiring minimal or no sample preparation. In plant science, LIBS adds another interesting applicability, which is the possibility to obtain spatial distribution information through elemental mapping. Spatial distribution allows to verify the uptake, translocation and accumulation of elements in different plant parts, resulting in studies of metabolic pathways and phytotoxicity studies. In front of this, in this work, seedlings of <em>Houttuynia cordata</em> Thunb. (<em>dokudami,</em> or fish-mint plant) were exposed via foliar application to solution of 600 μg L<sup>−1</sup> of Fe (Fe(NO<sub>3</sub>)<sub>3</sub>∙9H<sub>2</sub>O). The total concentration of Ca, Fe, K, and Mg in the control and Fe-exposed leaves were determined by ICP OES after digestion using microwave oven and diluted oxidant mixture. The elemental mapping by LIBS covered the entire abaxial surface of the leaf with 1.5 mm of distance between the ablation points, considering a predefined point-to-point pattern. The emission intensities in each region were used to elemental mapping construction. The emission intensity of C (247.856 nm) was applied as a correction standard. The Fe exposition promoted the Ca and K accumulation, while reducing Mg levels. The elemental variation was observed by LIBS mapping, revealing applicability in biological, as well as nutritional and toxicity studies.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128552"},"PeriodicalIF":5.6,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recognition of bioactive saponins from Polygonatum based on UPLC-QTOF/MS and molecular docking 基于UPLC-QTOF/MS和分子对接的黄精生物活性皂苷识别

IF 5.6 1区化学

Talanta Pub Date : 2025-07-05 DOI: 10.1016/j.talanta.2025.128485

Yunyun Zheng , Bingge Liu , Xiaojiang Wang , Xiaorui Wu , Pei Wang , Heng Sun , Yini Fu , Kouadio Jean Eric-Parfait Kouame , Xingqian Ye , Jianle Chen

{"title":"Recognition of bioactive saponins from Polygonatum based on UPLC-QTOF/MS and molecular docking","authors":"Yunyun Zheng , Bingge Liu , Xiaojiang Wang , Xiaorui Wu , Pei Wang , Heng Sun , Yini Fu , Kouadio Jean Eric-Parfait Kouame , Xingqian Ye , Jianle Chen","doi":"10.1016/j.talanta.2025.128485","DOIUrl":"10.1016/j.talanta.2025.128485","url":null,"abstract":"<div><div>Saponins are one of the main active ingredients in <em>Polygonatum</em>. This study aimed to identify <em>Polygonatum</em> saponins with superior biological properties from four species and screen for saponin compounds with high bioactivities. The results indicated that saponins content and bioactivity varied by species, especially PK, which demonstrated superior performance in both yield (extraction yield = 2.43 %, purification yield = 9.86 %) and bioactivities. Specifically, the IC<sub>50</sub> values for <em>α</em>-amylase and <em>α</em>-glucosidase were 0.877 mg/mL and 0.747 mg/mL, respectively. UPLC-QTOF/MS analysis further identified three saponins in PK, namely Sibiricoside B, Kingianoside C, and Kingianoside H, which demonstrated remarkable antioxidant activity in vitro. Molecular docking analyses revealed that Sibiricoside B (−8.051 kcal/mol) and Kingianoside H (−7.757 kcal/mol) exhibited significantly lower binding energies and shorter hydrogen bond distances compared to acarbose (−7.641 kcal/mol). These findings suggest that Sibiricoside B and Kingianoside H may serve as promising candidates for the development of natural inhibitors targeting type 2 diabetes mellitus, as well as potential active components in functional foods. Furthermore, all these findings also provide a basis for the valorization of <em>Polygonatum</em> species as natural resources, highlighting their potential to develop novel natural antioxidants and functional food ingredients.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128485"},"PeriodicalIF":5.6,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile fabrication of net-like fluorescent microsphere/AuNP tags for a dual-modal colorimetric/fluorescence lateral flow assay 用于双模态比色/荧光侧流分析的网状荧光微球/AuNP标签的简易制备

IF 5.6 1区化学

Talanta Pub Date : 2025-07-05 DOI: 10.1016/j.talanta.2025.128553

Xueguo Liu , Shasha Li , Yizhuo Zhao , Xueying Meng , Xiaojing Xing , Hong-Min Meng

{"title":"Facile fabrication of net-like fluorescent microsphere/AuNP tags for a dual-modal colorimetric/fluorescence lateral flow assay","authors":"Xueguo Liu , Shasha Li , Yizhuo Zhao , Xueying Meng , Xiaojing Xing , Hong-Min Meng","doi":"10.1016/j.talanta.2025.128553","DOIUrl":"10.1016/j.talanta.2025.128553","url":null,"abstract":"<div><div>Lateral flow assays (LFAs) offer a simple and rapid option for diagnosis, widely utilized for point-of-care or at-home testing. However, they often suffer from low sensitivity and high false positive owing to the single output signal. Herein, we developed a dual-mode colorimetry/fluorescence LFA <em>via</em> employing a novel net-like nanprobe as a reporter tag, which was obtained by coupling the fluorescent microspheres (FMs) with gold nanoparticles (AuNPs). Upon the introduction of the target (using miRNA-21 as a model), it hybridizes with the net-like nanprobe, subsequently trapped by the capture DNA immobilized on the test line, producing a prominent visual and fluorescent signal. Benefiting from the outstanding signal amplification capability of FMs/AuNPs nanoprobe, the developed dual-mode LFA enables highly sensitive detection of miRNA-21, with a detection limit (LOD) as low as 1 pM, which is 10 times and 20 times more sensitive than conventional FMs or AuNPs-based LFA, respectively. Additionally, in comparison to the LFA with single output signal, the analytical results of the dual-mode LFA are more reliable owing to the mutual verification of two different signals. Importantly, the platform can detect miRNA-21 in complex serum samples and distinguish cancer cells from normal cells in cell extract samples analysis, determining its great potential for point-of-care tests.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128553"},"PeriodicalIF":5.6,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel fluorescent ficin-templated Ni nanoclusters for nucleus imaging: a green and biocompatible alternative to traditional DNA stain 用于核成像的新型荧光纤维模板镍纳米簇：传统DNA染色的绿色和生物相容性替代品

IF 5.6 1区化学

Talanta Pub Date : 2025-07-05 DOI: 10.1016/j.talanta.2025.128548

Huili Cheng , Mingming Jin , Jinxin Fang , Ziheng Yuan , Yuan Wei , Baojuan Wang , Qingqing Huang

{"title":"Novel fluorescent ficin-templated Ni nanoclusters for nucleus imaging: a green and biocompatible alternative to traditional DNA stain","authors":"Huili Cheng , Mingming Jin , Jinxin Fang , Ziheng Yuan , Yuan Wei , Baojuan Wang , Qingqing Huang","doi":"10.1016/j.talanta.2025.128548","DOIUrl":"10.1016/j.talanta.2025.128548","url":null,"abstract":"<div><div>Fluorescence imaging of subcellular structures is crucial for understanding cellular functions and disease mechanisms. However, existing fluorescent dyes are plagued by inadequate biocompatibility and insufficient stability, which impede the advancement of high-resolution, long-term, and live-cell imaging. In this work, we innovatively synthesized a novel nickel nanocluster encapsulated with ficin (Ficin-Ni NCs) through a straightforward biomineralization approach as a fluorescent probe for imaging the nucleus. The Ficin-Ni NCs with intense green fluorescence exhibited a particle size of approximately 1.56 nm, significant Stokes shift (110 nm), and remarkable stability (98 % fluorescence intensity after 14 days). Meanwhile, the Ni NCs demonstrated excellent biocompatibility, maintaining cell viability above 90 % for both Raw264.7 and HEK-293T cells at a concentration of 60 μg/mL. Significantly, DNA gel electrophoresis experiments, using ethidium bromide (EB) as a reference, confirmed the ability of the NCs to bind DNA. Furthermore, the as-prepared Ficin-Ni NCs could achieve precise imaging of cell nuclei consistent with 4’,6-diamidino-2-phenylindole (DAPI) within 15 min in a variety of animal cells, including HEK-293T cells, Raw264.7, bullfrog erythrocytes, and human oral epithelial cells. Simultaneously, Ni NCs also demonstrated remarkable potential for visualizing the nuclear structures in plant cells, such as onion and cinnamon epidermal cells. Therefore, this innovative fluorescent probe, initially prepared via biomineralization, emerges as a promising alternative to traditional EB or DAPI dyes, paving the way for rapid response, effectiveness, and reusability in cellular imaging.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128548"},"PeriodicalIF":5.6,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly imprinted polymers based enrichment and separation for trace analysis in capillary electrophoresis 基于分子印迹聚合物的毛细管电泳富集与分离

IF 5.6 1区化学

Talanta Pub Date : 2025-07-05 DOI: 10.1016/j.talanta.2025.128549

Siyuan Peng , Yuhao Wen , Yongfei Ming , Tianzhou Huang , Gang Xu , Jingyi Yan , Jingying Huang , Zhihua Song , Weihua Wang , Michael Charles Breadmore , Lingxin Chen , Jinhua Li

{"title":"Molecularly imprinted polymers based enrichment and separation for trace analysis in capillary electrophoresis","authors":"Siyuan Peng , Yuhao Wen , Yongfei Ming , Tianzhou Huang , Gang Xu , Jingyi Yan , Jingying Huang , Zhihua Song , Weihua Wang , Michael Charles Breadmore , Lingxin Chen , Jinhua Li","doi":"10.1016/j.talanta.2025.128549","DOIUrl":"10.1016/j.talanta.2025.128549","url":null,"abstract":"<div><div>Capillary electrophoresis (CE) is well known for its high resolution, rapidity, low sample and reagent consumption, and ability to simply change separation modes, and has been widely used for separation and detection of various analytes important for many fields. One major limitation is the concentration limits of detection, which can be further worsened in complex samples with a significant matrix component. Molecularly imprinted polymers (MIPs) based solid-phase extraction (SPE) and stationary phases (SPs) enjoy great popularity in CE, owing to the recognition specificity, physical robustness, thermal stability, as well as low cost and easy preparation of MIPs. Therefore, herein, recent advances in MIPs-SPE and MIPs-SPs based enrichment and separation for trace analysis in CE are comprehensively reviewed. To the best of our knowledge, this work is the first review covering this topic centered on how MIPs improve CE performance, and provides meaningful advancement over the existing reviews. Three types of approaches to improving CE performance are discussed, including different CE modes, on/off-line molecularly imprinted SPE (MISPE) coupled with CE, and MIPs used as SPs in capillary. Subsequently, typical applications of MISPE and MIPs-SPs for CE determination of a variety of analytes in the fields of food safety, biological medicine and environmental monitoring are summarized. Finally, the possible challenges and perspectives, such as elaborate preparation of MIPs, rational selection of CE modes, pressing development of portable CE devices, are proposed, for promoting CE's detection capability and expanding its real-world applications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128549"},"PeriodicalIF":5.6,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogel-coated microneedle biosensor for selective and low-potential glucose detection in skin-mimicking interstitial fluid phantoms 水凝胶包被的微针生物传感器在皮肤模拟间质液幻象中用于选择性和低电位葡萄糖检测

IF 5.6 1区化学

Talanta Pub Date : 2025-07-05 DOI: 10.1016/j.talanta.2025.128555

Samira Mansouri Majd

{"title":"Hydrogel-coated microneedle biosensor for selective and low-potential glucose detection in skin-mimicking interstitial fluid phantoms","authors":"Samira Mansouri Majd","doi":"10.1016/j.talanta.2025.128555","DOIUrl":"10.1016/j.talanta.2025.128555","url":null,"abstract":"<div><div>The development of minimally invasive biosensors for continuous glucose monitoring has attracted considerable attention to improve patient compliance and comfort. In this study, a hydrogel-coated microneedle (MN) biosensor was designed and fabricated for the selective and low-potential electrochemical detection of glucose in skin-mimicking interstitial fluid (ISF) phantoms. Stainless steel MNs were modified with a glucose oxidase (GOx)-chitosan composite and further encapsulated within an alginate-polyvinyl alcohol (Alg-PVA) hydrogel matrix to enhance biocompatibility and analyte diffusion. The GOx immobilization method, the hydrogel composition, and the drying process were optimized. Cyclic voltammetry (CV) confirmed successful fabrication and efficient electron transfer. The biosensor exhibited a wide linear detection range of 1.0–50.0 mM with a limit of detection (LOD) of 0.11 mM in phosphate-buffered saline (PBS) and a linear range of 1.0–40.0 mM with an LOD of 0.20 mM in artificial ISF. The optimized low operating potential (−0.2 V) significantly minimized interference from common electroactive species such as ascorbic acid (AA), uric acid (UA), and acetaminophen (AP). Stability assessments demonstrated excellent repeatability (RSD <4 %) and reliable performance over a 14-day storage period. Furthermore, glucose measurements performed in skin-mimicking gel-based PBS and artificial ISF phantoms exhibited a linear response range of 1.0–25 mM and an LOD of 0.11 mM and linear response range of 1.0–40.0 mM and an LOD of 0.46 mM, respectively. These results demonstrate the practical applicability and reliability of the hydrogel-coated microneedle biosensor for accurate, painless, and real-time glucose monitoring under physiologically relevant conditions, highlighting its potential for wearable healthcare applications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128555"},"PeriodicalIF":5.6,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative quadrupolar NMR (qQNMR) determination of taurine in undiluted human urine samples using 1D and 2D 33S NMR 定量四极核磁共振（qQNMR）用1D和2D 33S核磁共振测定未稀释的人尿液样品中的牛磺酸

IF 5.6 1区化学

Talanta Pub Date : 2025-07-05 DOI: 10.1016/j.talanta.2025.128556

Arantxa Forte-Castro, Ana Belén Ruiz-Muelle, Ignacio Fernández

引用次数: 0