Talanta最新文献_第10页

Dual-mode exosome detection leveraging a nanozyme-active artificial receptor: PDA@Fe@Zn-based nucleic acid aptamer sensor. 利用纳米酶活性人工受体的双模式外泌体检测：PDA@Fe@锌基核酸适体传感器。

IF 5.6 1区化学

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-21 DOI: 10.1016/j.talanta.2024.127380

Linghao Zhao, Jingjing Kuang, Keheng Xiang, Jiayue Gan, Yuxin Zeng, Xichen Zhang, Yicheng Yan, Min Zhang, Hongyang Zhang, Ping Hu

{"title":"Dual-mode exosome detection leveraging a nanozyme-active artificial receptor: PDA@Fe@Zn-based nucleic acid aptamer sensor.","authors":"Linghao Zhao, Jingjing Kuang, Keheng Xiang, Jiayue Gan, Yuxin Zeng, Xichen Zhang, Yicheng Yan, Min Zhang, Hongyang Zhang, Ping Hu","doi":"10.1016/j.talanta.2024.127380","DOIUrl":"10.1016/j.talanta.2024.127380","url":null,"abstract":"<p><p>Exosomes, extracellular vesicles crucial for intercellular communication, are emerging as significant biomarkers for disease diagnosis, especially in cancer. This study presented a dual-mode exosome detection platform using polydopamine microspheres doped with iron and zinc ions (PDA@Fe@Zn). These materials served as both artificial receptors for nucleic acid aptamers and nanozymes with peroxidase-like activity. By integrating colorimetric and fluorescence detection, the platform enables cross-validation of results. PDA@Fe@Zn nanozymes catalyzed the TMB-H<sub>2</sub>O<sub>2</sub> reaction under acidic conditions, producing a colorimetric signal proportional to exosome concentration. Concurrently, the fluorescence of FAM-labeled aptamers was dynamically quenched by PDA@Fe@Zn and the presence of exosomes restores the fluorescence signal for a \"turn-on\" detection mode. DNase I amplified detection signals by cleaving bound exosomes for multiple cycles, achieving a limit of detection (LOD) of 4.7 × 10<sup>4</sup> particles/mL for colorimetric detection and 2.2 × 10<sup>4</sup> particles/mL for fluorescence detection. Notably, the colorimetric platform revealed that the relative expression of the CD63 protein on exosomes from breast cancer cells MCF-7 and MDA-MB-231 was approximately 2.7 and 2.4 times higher, respectively, than in normal breast cells MCF-10A; similar fold changes of 2.9 and 2.4 were observed with fluorescence detection, underscoring the robustness of the dual-mode system. The platform demonstrated rapid detection (within 30 min), high sensitivity, strong anti-interference capability, and the ability to distinguish exosomes from cancerous and normal cells effectively.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127380"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid on-site diagnosis of PEDV and PoRV co-infection by gold magnetic nanoparticles-based SERS immunochromatography. 基于金磁性纳米粒子的SERS免疫层析快速现场诊断PEDV和PoRV共感染。

IF 5.6 1区化学

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-19 DOI: 10.1016/j.talanta.2024.127428

Zhaoyuan He, Xiaoge Duan, Zhi Zhao, Yingkai Chen, Chengxiang Fu, Fuxin Zhang, Jinzi Wang, Jianyuan Feng, Nanxin Lin, Hailan Chen

{"title":"Rapid on-site diagnosis of PEDV and PoRV co-infection by gold magnetic nanoparticles-based SERS immunochromatography.","authors":"Zhaoyuan He, Xiaoge Duan, Zhi Zhao, Yingkai Chen, Chengxiang Fu, Fuxin Zhang, Jinzi Wang, Jianyuan Feng, Nanxin Lin, Hailan Chen","doi":"10.1016/j.talanta.2024.127428","DOIUrl":"10.1016/j.talanta.2024.127428","url":null,"abstract":"<p><p>Porcine epidemic diarrhea virus (PEDV) and porcine rotavirus (PoRV) are the two main pathogens causing porcine diarrhea, which are characterized by high morbidity and mortality. Most of the diagnostic methods available are limited to the laboratory or fail to highlight their advantages in terms of target species, detection time, sensitivity, and stability. To meet the demand for rapid on-site diagnosis of PEDV and PoRV co-infection, a surface-enhanced Raman scattering (SERS) immunochromatographic sensor based on gold magnetic nanoparticles (MNPs) was developed. The sensor is dual-mode, detecting on the basis of color signals by the naked eye and Raman signals. After a series of optimizations, the constructed sensor could perform simultaneous qualitative and quantitative detection of PEDV and PoRV in just 18 min, with visualized (color signals observed by the naked eye) limits of detection (LOD) of 3.13 × 10<sup>2</sup> TCID<sub>50</sub>/mL and 4.69 × 10<sup>2</sup> copies/μL, respectively. The LOD based on Raman signal analysis was as low as 4.63 × 10<sup>1</sup> TCID<sub>50</sub>/mL and 3.30 × 10<sup>2</sup> copies/μL for PEDV and PoRV, respectively. In addition, the sensor exhibited excellent specificity without cross-reactivity with common pathogens. The overall compliance rate with RT-PCR was 92.1 % (35/38) for 38 clinical samples. Therefore, the sensor is characterized by high sensitivity, specificity, reproducibility, and accuracy, making it suitable for the simultaneous rapid on-site detection of PEDV and PoRV.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127428"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A smartphone-based dual-modal lateral flow immunochromatographic assay for multiplex detection of illegal stimulant laxatives in slimming foods. 基于智能手机的双模态横向流动免疫层析检测减肥食品中非法兴奋剂泻药的多重检测。

IF 5.6 1区化学

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-21 DOI: 10.1016/j.talanta.2024.127433

Shuiyuan Liang, Sha Chen, Zhenlin Xu, Jiahong Chen, Hongtao Lei, Tian Guan

{"title":"A smartphone-based dual-modal lateral flow immunochromatographic assay for multiplex detection of illegal stimulant laxatives in slimming foods.","authors":"Shuiyuan Liang, Sha Chen, Zhenlin Xu, Jiahong Chen, Hongtao Lei, Tian Guan","doi":"10.1016/j.talanta.2024.127433","DOIUrl":"10.1016/j.talanta.2024.127433","url":null,"abstract":"<p><p>Stimulant laxatives (especially bisacodyl and sodium picosulfate) are frequently found to be adulterated into slimming foods, causing health-threatening effects to consumers. Sensitive, accurate, easy-to-operate and portable multiplex analytical techniques are still desired for the rapid screening of stimulant laxatives in slimming foods. In this work, a highly sensitive dual-modal colorimetric/photothermal lateral flow immunochromatographic assay (LFIA) was established based on facilely prepared concentrated gold nanoparticles (cAuNPs). Due to the enhanced local surface plasmon resonance and absorption intensity of our unique cAuNPs, this LFIA can simultaneously detect bisacodyl, bis-(p-hydroxyphenyl)-pyridyl-2-methane and sodium picosulfate in slimming food as low as 0.014-0.308 ng/mL by a smartphone-based photothermal device or 1.4-10 ng/mL by naked eyes. Compared with traditional AuNPs-LFIA, it demonstrated 8.48-10.27 times higher sensitivity in photothermal mode. Moreover, in the recovery test and blind sample analysis, this dual-modal LFIA exhibited reasonable recoveries (85.04 %-119.18 %) and high correlation with authorized LC-MS/MS method, suggesting high accuracy and applicability. This work offers a highly sensitive, accurate and convenient way for the on-site determination of stimulant laxatives, which can also be employed to detect other food hazards, environmental pollutants, or clinical analytes within a few minutes.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127433"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive trypsin sensor based on the regulation of microscale ionic current rectification by the selectivity hydrolysis of hydrogel filled in microchannel. 灵敏胰蛋白酶传感器基于微尺度离子电流的调节，通过选择性水解填充在微通道中的水凝胶进行整流。

IF 5.6 1区化学

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-21 DOI: 10.1016/j.talanta.2024.127422

Huabin Cai, Runhao Yuan, Shaokun Huang, Yanling Huang, Cuiying Lin, Yue Lin, Fang Luo, Zhenyu Lin, Lixin Wang

{"title":"Sensitive trypsin sensor based on the regulation of microscale ionic current rectification by the selectivity hydrolysis of hydrogel filled in microchannel.","authors":"Huabin Cai, Runhao Yuan, Shaokun Huang, Yanling Huang, Cuiying Lin, Yue Lin, Fang Luo, Zhenyu Lin, Lixin Wang","doi":"10.1016/j.talanta.2024.127422","DOIUrl":"10.1016/j.talanta.2024.127422","url":null,"abstract":"<p><p>Filling the microchannel with negatively charged hydrogel can exhibit microsacle ion current rectification (ICR) behavior, which is attributed to the space negative charge and structural asymmetry of hydrogel. In this study, this character had been applied to develop a trypsin sensor for the first time. A hydrogel synthesized with bovine serum albumin (BSA) and glyoxal (BSAG hydrogel) was filled at the tip of microchannel firstly. Subsequently, the BSAG hydrogel-filled microchannel was immersed in a trypsin solution to hydrolyze the BSA within the BSAG hydrogel. This process changes the space charge density and pore size of the BSAG hydrogel-filled microchannel, leading to a change in microscale ICR, which can be used for quantifying trypsin. Then the key parameters affecting the sensing performance such as the concentration of BSA, strength of the electrolyte, pH and reaction time were optimized. The detection range was from 10.0 ng/mL to 100 μg/mL with a detection limit as low as 2.55 ng/mL (S/N = 3). Due to the distinctive three-dimensional pore structure of the hydrogel and the specificity of trypsin for BSA hydrolysis, the sensor exhibits high sensitivity and specificity, as well as remarkable reproducibility and stability. This sensor has been effectively used to measure trypsin levels in human serum samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127422"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azo-PMA nanopores of sub-20 nm length for unimolecular resolution of nucleic acids and proteins. 亚20 nm长度的偶氮聚甲基丙烯酸甲酯纳米孔用于核酸和蛋白质的单分子分辨率。

IF 5.6 1区化学

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-16 DOI: 10.1016/j.talanta.2024.127402

Guodong Qi, Xun Chen, Daixin Liu, Meili Ren, Rong Tian, Liyuan Liang

{"title":"Azo-PMA nanopores of sub-20 nm length for unimolecular resolution of nucleic acids and proteins.","authors":"Guodong Qi, Xun Chen, Daixin Liu, Meili Ren, Rong Tian, Liyuan Liang","doi":"10.1016/j.talanta.2024.127402","DOIUrl":"10.1016/j.talanta.2024.127402","url":null,"abstract":"<p><p>Owing to the facile fabrication and surface modification, the cost-effective polymer nanopores are widely employed in unimolecular determination of biomacromolecules and selective sensing of small molecules, nanoparticles and biomarkers. However, the documented polymer nanochannels are generally microscale in length with low spatial resolution. We herein synthesized azobenzene side-chain polymer (Azo-PMA) and spin-coated on silicon nitride membrane to obtain a polymer film of nanoscale thickness for further nanopore generation via controlled dielectric breakdown (CDB) approach. The Azo-PMA nanopores demonstrate good ions transporting activities, pH tolerance and stability in high concentration of electrolyte with low ionic current noise. In addition, the azobenzene-containing polymer nanopores exhibit photo-response upon UV/Vis. light irradiation. The Azo-PMA nanopore devices are utilized for linear and quadruple nucleic acids discrimination, sensing of proteins with distinct shapes and sizes, as well as the single amino acid resolution with good capture rate and sensitivity. We established an unimolecular sensing platform using polymer nanopores for nucleic acids and proteins detection with good spatial resolution, which will be an addition for the nanopore-carrier material exploration and applications in potential genomics and proteomics with high spatiotemporal resolution and low cost.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127402"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple colorimetric and paper-based-smartphone sensing platform based on the enhanced peroxidase-like activity of Al doping Prussian blue for point-of-care detection of GSH

IF 5.6 1区化学

Talanta Pub Date : 2025-03-30 DOI: 10.1016/j.talanta.2025.128020

Ying Yang , Liuyan Wei , Weiqin You, Huisha Huang, Shuai Wang, Dandan Hou, Chujun Qin, Zhiheng Su, Mei Li

{"title":"A simple colorimetric and paper-based-smartphone sensing platform based on the enhanced peroxidase-like activity of Al doping Prussian blue for point-of-care detection of GSH","authors":"Ying Yang , Liuyan Wei , Weiqin You, Huisha Huang, Shuai Wang, Dandan Hou, Chujun Qin, Zhiheng Su, Mei Li","doi":"10.1016/j.talanta.2025.128020","DOIUrl":"10.1016/j.talanta.2025.128020","url":null,"abstract":"<div><div>Glutathione (GSH) is a very important antioxidant and also participates in many important physiological processes, accurate determination of GSH in food and blood fluid is crucial for human health. Herein, we introduce a simple colorimetric and paper-based-smartphone dual signal output platform for point-of-care (POC) detection of GSH in garlic and human serum. The experiment revealed that the peroxidase-like activity of Aluminum-doped Prussian blue (AlPB) could be enhanced by the doping of Al element on Prussian blue. AlPB exhibited excellent peroxidase-like activity and efficiently catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H<sub>2</sub>O<sub>2</sub> to generate blue oxidized TMB, resulting in an absorbance and color-based-smartphone dual signal readouts. Enzyme kinetic studies also indicated that the AlPB showed high affinity towards TMB. Hence, a reliable colorimetric and color-based-smartphone dual signal readouts assay was constructed based on AlPB-mediated the peroxidase-like activity, it was used for highly sensitive colorimetric and POC detection of GSH in food and human serum within 7 min with a wide range of linear response from 1 to 20 μM, a low detection limit of 0.1748 μM. This work demonstrates a novel and versatile strategy to develop superior peroxidase mimics and holds great potential for rapid and portable detection of GSH in food, healthcare and clinical diagnosis, and also open promising avenues for more powerful paper-based POC tests.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128020"},"PeriodicalIF":5.6,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controllable construction of ZnSnO3 polyhedrons abundant in oxygen vacancies via cobalt doping and their acetone sensing performance

IF 5.6 1区化学

Talanta Pub Date : 2025-03-29 DOI: 10.1016/j.talanta.2025.128051

Cheng Chen , Jiarong Chen , Yibo Wang , Zhen Han , Wen Zeng

{"title":"Controllable construction of ZnSnO3 polyhedrons abundant in oxygen vacancies via cobalt doping and their acetone sensing performance","authors":"Cheng Chen , Jiarong Chen , Yibo Wang , Zhen Han , Wen Zeng","doi":"10.1016/j.talanta.2025.128051","DOIUrl":"10.1016/j.talanta.2025.128051","url":null,"abstract":"<div><div>Increasing attentions have been paid to the development of ZnSnO<sub>3</sub>-based gas sensors for acetone detection, owing to their unique advantages of high electron mobility, low visible adsorption and excellent electrical conductivity. However, some intrinsic problems associated with low response remain to be satisfactorily resolved. Herein, cobalt (Co) doping was implemented in this work to simultaneously modulate the electronic structure and oxygen vacancy of ZnSnO<sub>3</sub>, a series of ZnSnO<sub>3</sub> doped with varying quantities of Co ions were harvested through a hydrothermal method. The results reveal that the sensor, fabricated using ZnSnO<sub>3</sub> with the incorporation of 10 mol% Co ions, manifests exceptional response (109) to 100 ppm acetone at 260 °C, accompanied by a speedy response/recover time (21/11 s). Furthermore, the gas device exhibits excellent comprehensive properties, including an ultra-low theoretical limit of detection (25 ppb), reliable long-term stability, as well as good anti-humidity performance. It is concluded by a series of characterization techniques that large BET surface area and abundant oxygen vacancies conduce to the significant enhancement in sensing performance. This study sheds light on fundamental understanding of how to tailor high-performance ZnSnO<sub>3</sub>-based sensing devices for toxic gas detection.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128051"},"PeriodicalIF":5.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RT-RPA-assisted scaffold RNA transcription amplification activation Cas12a trans-cleavage strategy for one-pot miRNA detection

IF 5.6 1区化学

Talanta Pub Date : 2025-03-29 DOI: 10.1016/j.talanta.2025.128049

Cheng-Jun Zha , Hong-Shuai Zhang , Sheng He , Na Zhao , Liang He , Nan Yang , Zhan-Ming Ying

{"title":"RT-RPA-assisted scaffold RNA transcription amplification activation Cas12a trans-cleavage strategy for one-pot miRNA detection","authors":"Cheng-Jun Zha , Hong-Shuai Zhang , Sheng He , Na Zhao , Liang He , Nan Yang , Zhan-Ming Ying","doi":"10.1016/j.talanta.2025.128049","DOIUrl":"10.1016/j.talanta.2025.128049","url":null,"abstract":"<div><div>Split crRNA is utilized in the CRISPR system as a highly specific and sensitive tool for nucleic acid identification in molecular diagnostics. Here, we introduce a novel one-pot RT-RPA-assisted Scaffold RNA Transcription Amplification Activation Cas12a <em>Trans</em>-cleavage (ScRNA-TAAT) strategy for miRNA detection. Capable of completing miRNA detection in 50 min with a detection limit of 2.66 aM. The miRNA was amplified into double-stranded DNA amplicons with a T7 promoter using RT-RPA, which is subsequently transcribed into RNA trigger. A split T7 promoter extension sequence with template binds to the RNA trigger, assembly a three-way linker to initiate transcription of the scaffold RNA. The three-way linker with sticky ends joins to its RNA products, assembling a DNA-RNA complex that acts as both a spacer RNA and an activator. Simultaneously, the DNA-RNA hybrid complexes and scaffold RNA could replace the split crRNA and combined with Cas12a, forming an active ribonucleoprotein (RNP) complex with <em>trans</em>-cleavage activity comparable to that of the wild-type Cas12a RNP. Furthermore, we have successfully integrated the ScRNA-TAAT strategy into lateral flow analysis, enabling the visual detection of miRNAs. The assay's exceptional specificity and universality are highlighted by its application to complex biological matrices, including serum and cell lysates. The simplicity, sensitivity, and adaptability of the ScRNA TAAT assay render it a promising candidate for point-of-care testing and high sensitivity in molecular diagnostics.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128049"},"PeriodicalIF":5.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive and smart trace detection via interface-enhanced multi-phonon resonance Raman scattering

IF 5.6 1区化学

Talanta Pub Date : 2025-03-29 DOI: 10.1016/j.talanta.2025.128047

Yao Jin , Zhipeng Li , Kaikai Ba , Xiaokun Wen , Zhibo Xue , Huan Wang , Tengfeng Xie , Jun Li , Xia Hong , Yichun Liu

{"title":"Ultrasensitive and smart trace detection via interface-enhanced multi-phonon resonance Raman scattering","authors":"Yao Jin , Zhipeng Li , Kaikai Ba , Xiaokun Wen , Zhibo Xue , Huan Wang , Tengfeng Xie , Jun Li , Xia Hong , Yichun Liu","doi":"10.1016/j.talanta.2025.128047","DOIUrl":"10.1016/j.talanta.2025.128047","url":null,"abstract":"<div><div>Trace detection technology has important applications in fields such as biomedicine, food safety, and environmental monitoring. However, it currently faces challenges including insufficient sensitivity, low efficiency, and a need for improved personalized services, which seriously hinder the in-depth development and widespread application of trace detection technology. Herein, a metal/semiconductor heterojunction was designed and constructed. The synergistic effects of interfacial charge transfer and interfacial passivation of anion vacancy defect states density were revealed to significantly enhance the intensity of multi-phonon resonance Raman scattering (MRRS) of the semiconductor with an enhancement factor of up to 1.4 × 10<sup>9</sup>. A MRRS detection platform was developed to achieve a detection limit of 8.5 aM for let-7a and a relatively wide linear range from 50 aM to 100 nM. Compared to conventional semiconductor detection platforms, the heterojunction probes reduced the detection limit by three orders of magnitude, and expanded the linear range by five orders of magnitude. Furthermore, the enhanced synergistic effect at the heterojunction interface directly boosts the MRRS signal intensity, significantly lowering the detection limit, and broadening the linear range. To further improve detection efficiency and user experience, this technology was combined with smartphone intelligent analysis software, allowing for rapid reading and intelligent analysis of MRRS data from serum sample, which enhances the speed and accuracy of data processing. This work not only offers a new, ultrasensitive approach to develop trace detection technology, but also makes significant strides in improving personalized services and promoting the advancement of telemedicine.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128047"},"PeriodicalIF":5.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deformation-resistant coaxial fiber photoelectrochemical sensor with vertical anchoring of graphene nanosheets for ultrasensitive glucose detection

IF 5.6 1区化学

Talanta Pub Date : 2025-03-29 DOI: 10.1016/j.talanta.2025.128048

Yuanyuan Zhang , Chunjing Zhang , Wenhao Guo , Yatao Chang , Jinpeng Liu , Tongtong Qin , Dapeng Liu , Liming Zhao , Zhengpeng Yang , Yongyi Zhang

{"title":"Deformation-resistant coaxial fiber photoelectrochemical sensor with vertical anchoring of graphene nanosheets for ultrasensitive glucose detection","authors":"Yuanyuan Zhang , Chunjing Zhang , Wenhao Guo , Yatao Chang , Jinpeng Liu , Tongtong Qin , Dapeng Liu , Liming Zhao , Zhengpeng Yang , Yongyi Zhang","doi":"10.1016/j.talanta.2025.128048","DOIUrl":"10.1016/j.talanta.2025.128048","url":null,"abstract":"<div><div>Endowing microelectrode architecture with eminent light-absorbing, analyte-trapping and mechanical robustness is pivotal but challenging for state-of-the-art photoelectrochemical monitoring. Herein, an effective tactic to tackle these issues was proposed for building coaxial fiber-shaped photoelectrochemical sensor, with multiscale vertically oriented channels created by highly ordered arrangement of molecule-recognized graphene (G) nanosheets serving as photoexcitation initiator along the direction perpendicular to the core-layer carbon nanotube (CNT) fiber acting as supporting and conductive matrix. The unique architectural features enabled rapid analyte diffusion and ready light spreading to photoactive and specific recognition sites situated at all channel walls, and meanwhile rendered the device with robust structural integrity, thereby showcasing impressive glucose-assaying capability with rapid response (0.3 s), low detection limit (0.7 μM), wide linear range (4–180 μM), good long-time stability (more than 60 days), superior selectivity and deformation endurance. This work opens up a promising route for processing advanced microelectrode architectures toward highly sensitive and selective photoelectrochemical monitoring even under harsh deformations.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128048"},"PeriodicalIF":5.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0