Talanta最新文献

{"title":"Ratiometric fluorescence sensing and discrimination of tetracycline analogs by using coumarin-embedded Eu-MOF nanosensor.","authors":"Peng-Xiang Yuan, Yu-Ping Wang, Fangfang Du, Liu-Pan Yang, Li-Li Wang","doi":"10.1016/j.talanta.2024.126914","DOIUrl":"10.1016/j.talanta.2024.126914","url":null,"abstract":"<p><p>As widely used antibiotics, tetracycline residues exist in food and environmental media, which pose certain hidden dangers and negative effects on public health. Therefore, the sensing and discrimination of tetracycline analogs (TCs) have great significance for improving food safety and preventing environmental pollution. Herein, a 7-hydroxycoumarin-3-carboxylic acid-embedded Eu-MOF (HC@Eu-MOF) material was constructed and then developed for the detection of TCs. Upon addition of TCs, the synthesized sensor displays opposite fluorescence changes at two different wavelengths due to the simultaneous presence of the inner filter effect (IFE) and the antenna effect (AE), and achieves a stable ratio signal response within 90 s. In addition, six important tetracycline analogs, including chlortetracycline (CTC), oxytetracycline (OTC), tetracycline (TC), metacycline (MC), doxycycline (DC) and demeclocycline (DMC) can be discriminated with 100 % accuracy through the principal component analysis even in extremely complicated mixtures. Further, a smartphone-assisted portable device was applied for visual sensing of TCs. The as-developed platform possessed the characteristics of simple synthesis, fast response, high sensitivity, and high stability, which further lays a further foundation for the on-site visual detection and discrimination of TCs in real samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126914"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA.","authors":"Anna Andrew, Magdline S H Sum, Ewe Seng Ch'ng, Thean-Hock Tang, Marimuthu Citartan","doi":"10.1016/j.talanta.2024.126842","DOIUrl":"10.1016/j.talanta.2024.126842","url":null,"abstract":"<p><p>Chikungunya fever, caused by Chikungunya virus (CHIKV) exhibits clinical features that mimic that of other arbovirus infections such as dengue. CHIKV Envelope 2 (E2) protein, an antigenic epitope of CHIKV, has been identified as an ideal marker for diagnostics. The current CHIKV antigen detection tests are largely based on antibodies but are beleaguered by issues such as sensitivity to high temperature, expensive and prone to batch-to-batch variations. Aptamers are suitable alternatives to antibodies as they are cheaper and have no batch-to-batch variations compared to antibodies. In this study, DNA aptamer selection against CHIKV E2 proteins was performed using two different randomized ssDNA libraries. Chik-2 (96-mer) and Chik-3 (76-mer) were isolated from these two libraries and were identified as the potential aptamers against CHIKV E2 protein. The binding affinity of Chik-2 and Chik-3 against CHIKV E2 protein was estimated at 177.5 ± 32.69 nM and 30.01 ± 3.60 nM, respectively. A sandwich ELASA was developed, and this assay showed a detection limit of 2.17 x 10³ PFU/mL. The sensitivity and specificity of the assay were 80 % and 100 %, respectively. The assay showed no cross-reactivity with dengue-positive samples, demonstrating the enormous diagnostic potential of these aptamers for the detection of CHIKV.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126842"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fe₃O₄@nitrogen-doped carbon@Pd core-double shell nanotubes as a novel nanosorbent for ultrasonic assisted dispersive magnetic solid phase extraction of organophosphorus pesticides.","authors":"Zolfaghar Aladaghlo, Ali Sahragard, Alireza Fakhari, Neda Salarinejad, Siyavash Kazemi Movahed, Minoo Dabiri","doi":"10.1016/j.talanta.2024.126911","DOIUrl":"10.1016/j.talanta.2024.126911","url":null,"abstract":"<p><p>In this study, an ultrasonic assisted dispersive magnetic solid phase extraction leveraging Fe₃O₄@nitrogen-doped carbon@Pd core-double shell nanotubes was developed for the extraction of organophosphorus pesticides (OPPs) in trace levels from real samples. Incorporation of Pd species into the structure of the nanosorbent could enhance its interactions with sulfur groups in the structure of OPPs. X-ray photoelectron spectroscopy and X-ray diffraction, brunauer-emmett-teller, field emission scanning electron microscopy, and high-resolution transmission electron microscopy were used to characterize the nanosorbent after its synthesis. Then, effective variables on the extraction efficiency of OPPs using the nanosorbent were optimized. These parameters included 2-propanol as the adsorption solvent; the sample pH of 7.0; the sorbent quantity of 10 mg; and the extraction and desorption times of 3 min. Under optimized conditions, linear ranges with determination coefficients (R²) higher than 0.99, low detection limits of 0.30 ng mL^-1, high preconcentration factors (423-470) and relatively high extraction recoveries (84-94 %) were obtained. The proposed extraction system was then successfully applied to the analysis of OPPs in fruits, vegetables, water, and agricultural soil samples, yielding relative recoveries from 90.4 to 107 %.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126911"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of transferrin uptake into living cells at single-molecule level.","authors":"Marta Pilz, Tomasz Kalwarczyk, Krzysztof Burdzy, Robert Hołyst","doi":"10.1016/j.talanta.2024.127031","DOIUrl":"10.1016/j.talanta.2024.127031","url":null,"abstract":"<p><p>Endocytosis, apart from providing a mechanism for cells to take up nutrients, is the principal route of entry for many nanomedicines. The evaluation of therapeutic delivery efficiency without providing quantitative parameters, like the number of molecules entering the cell and the time of their entry, is very limited. Despite advances in single-molecule methods for in-cell quantitative measurements, they have not become widely used in the study of endocytosis. Their application, however, is challenging owing to the appropriate experimental design and applicable analysis methods. Here, by using an integrated approach based on the multidimensional time-correlated single-photon counting (TCSPC) technique, we demonstrate the quantitative method to measure the time- and concentration-dependent uptake of TRITC-transferrin into living cells with single-molecule sensitivity. The methodology opens possibilities for quantitatively assessing the delivery efficiency of fluorescent molecules into living cells.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127031"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering of in-plane SnS₂-SnO₂ nanosheets heterostructures for enhanced H₂S sensing.","authors":"Xuan-Yu Yang, Jian-Yong Yuan, Yang Ye, Li-Juan Yue, Fei-Long Gong, Ke-Feng Xie, Yong-Hui Zhang","doi":"10.1016/j.talanta.2024.127059","DOIUrl":"10.1016/j.talanta.2024.127059","url":null,"abstract":"<p><p>In-plane heterostructures has attracted considerable interest due to exceptional electron transport properties, high specific surface area, and abundant active sites. However, synthesis of in-plane SnS₂-SnO₂ heterostructures are rarely reported, and the deep investigation of the fine structure on reactivity is of great significance. Here, we propose partial in-situ oxidation strategy to construct the in-plane SnS₂-SnO₂ heterostructures and the surface properties, the ratio of two components can be finely tuned by precisely adjusting the treatment temperature. In particular, the SnS₂-SnO₂ heterostructures formed after annealing of SnS₂ nanosheets at 350 °C exhibits a unique electronic structure and surface properties due to rich grain boundaries, which exhibits excellent gas sensing performance to H₂S (R_a/R_g = 169.81 for 5 ppm H₂S at 160 °C, fast response and recovery dynamic (41/101 s), excellent reliability (σ = 0.01) and sensing stability (φ = 0.11 %)). Notably, the in-plane heterostructures endow the material with abundant grain boundaries and effectively regulates the electronic structure of the Sn p-orbital, which facilitate the formation of active oxygen species (O^-(ad)), thus contributing to the sensing performance. Our work provides a promising platform to design in-plane heterostructures for various advanced applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127059"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microgalvanic cell-mediated green synthesis of Cu₂O nanocubes with (100) facets for boosting dopamine hydrochloride sensing performance.","authors":"Yiyue Ma, Xintong Wu, Haoyu Pan, Wenxin Zhu, Chengyi Lu, Jianlong Wang","doi":"10.1016/j.talanta.2024.126995","DOIUrl":"10.1016/j.talanta.2024.126995","url":null,"abstract":"<p><p>Despite numerous efforts have been made on exploring the preparation, properties and application of Cu₂O nanocrystal, there is still a lack of a facile and green synthesis strategy to obtain well-defined Cu₂O nanocubes (NCs). And exploration of the superior low-index lattice plane of Cu₂O in electrochemical sensing is also inadequate. Herein, we proposed a Ni(OH)₂-mediated in-situ synthetic strategy for the preparation of Cu₂O NCs enclosed by low-index facets with simple procedure, mild temperature and low energy-consumption. The Ni(OH)₂ sites not only facilitated the contact between Cu²⁺ and the substrate Ni foam (NF), but also can combine with the NF to act as a primary battery to regulate the nucleation and growth rate of Cu₂O (100) facets. Benefiting from the high ratio of exposed electroneutral (100) lattice planes of nanocubes, the Cu₂O NCs formed on Ni(OH)₂-abundant Ni Foam (Cu₂O NCs/NF_EO) exhibited a wide linear range (3.25-1178.8 μM), a low detection limit (1.86 μM) and a high sensitivity (900 μA mM^-1 cm^-2) in dopamine hydrochloride (DAH) electrochemical sensing. This work expects to provide more clues about the relationship between different dominant low-index facets of Cu₂O NCs and electrochemical sensing performance towards DAH, and thereby contributes to the development of functional materials based on Cu₂O nanocrystals with desirable facets.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"126995"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared-driven dual-photoelectrode photoelectrochemical sensing for fumonisin B1: Integrating a photon up-conversion bio-photocathode with an enhanced light-capturing photoanode.","authors":"Jiang Guo, Xuqiao Liu, Jianqiao Liu, Kai Yan, Jingdong Zhang","doi":"10.1016/j.talanta.2024.127047","DOIUrl":"10.1016/j.talanta.2024.127047","url":null,"abstract":"<p><p>Fumonisin B1 (FB1), the most prevalent and highly toxic mycotoxin within the fumonisins family, poses threats to humans, especially in children and infants, even at trace levels. Therefore, it is essential to design an easy and sensitive detection strategy. Herein, a brand-new dual-photoelectrode photoelectrochemical (PEC) sensing platform for FB1 detection under near-infrared irradiation was unveiled. This platform integrated a photon up-conversion bio-photocathode substrate (UCNPs/Au/CuInS₂, UCNPs: NaYF₄: Yb³⁺, Er³⁺, Nd³⁺) and used a SnO₂/SnS₂@Bi/Bi₂S₃ heterojunction photoanode to greatly enhance light capture. Additionally, ZnO coated with polydopamine (ZnO@PDA) was utilized as a signal inhibitor. The restoration of photocurrent occurred due to the strong binding affinity between FB1 and its aptamer (FB1-Apt), facilitating the dissociation of FB1-Apt/ZnO@PDA from the photoelectrode. The PEC sensing performance and the electron transfer process were thoroughly examined. The developed \"signal-restoration\" PEC aptasensor exhibited a wider dynamic linear range from 1.0 × 10^-3 to 1.0 × 10² ng/mL, with a lower limit of detection (0.13 pg/mL). It has demonstrated excellent practical detection performance in unspiked real samples, such as corn paste, with the FB1 enzyme-linked immunosorbent assay (ELISA) Kit serving as a reference, indicating its potential for routine analysis of other mycotoxins. Thus, this research establishes a feasible dual-photoelectrode PEC framework for the effective detection of mycotoxins and other hazardous substances.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127047"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-pronged strategy: A mitochondria targeting AIE photosensitizer for hydrogen sulfide detection and type I and type II photodynamic therapy.","authors":"Jizhong Shi, Manshan Liang, Yiting Qiu, Jin Zhang, Shihua Wang, Heng Fang, Yongsheng Jiang, Xiaoxia Ye, Yanshu Luo, Zu-Sheng Huang, Yun-Yun Quan","doi":"10.1016/j.talanta.2024.127074","DOIUrl":"10.1016/j.talanta.2024.127074","url":null,"abstract":"<p><p>Multifunctional type-I photosensitizers (PSs) for hydrogen sulfide (H₂S) detection and photodynamic therapy (PDT) of hypoxia tumors exhibits attractive curative effect but remains a challenging task. Herein, a mitochondria targeted aggregation-induced emission (AIE) photosensitizer TSPy-SS-P was designed and synthesized, which could be used for H₂S detection and simultaneously type I and type II PDT. TSPy-SS-P had excellent selectivity and anti-interference abilities for endogenous and exogenous H₂S detection in tumor cells. TSPy-SS-P was able to distinguish tumor cells with high level of H₂S from normal cells by fluorescence \"turn off\" response to H₂S. In addition, TSPy-SS-P showed type Ⅰ and type Ⅱ reactive oxygen species (ROS) generation ability to effectively ablate hypoxic tumor cells. TSPy-SS-P showed mitochondria targeting capacity which could produce ROS in situ to disrupt mitochondria and promote cell apoptosis. In vivo PDT experiments showcased that TSPy-SS-P had excellent tumor retention capability, effective tumor ablation ability and good biocompatibility. This work provided a two-pronged strategy to design organelles targeted photosensitizers for H₂S detection and effective PDT of tumors.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127074"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple and accurate method for the determination of Rh, Pd, and Pt in e-waste and spent automotive catalysts using HR-CS FAAS for assessing the value of secondary raw materials.","authors":"Magda Zabielska-Konopka, Elżbieta Zambrzycka-Szelewa, Zofia Kowalewska, Beata Godlewska-Żyłkiewicz","doi":"10.1016/j.talanta.2024.126894","DOIUrl":"10.1016/j.talanta.2024.126894","url":null,"abstract":"<p><p>This work presents a simple and accurate method for the fast sequential determination of Rh, Pd, and Pt in spent automotive catalysts and e-wastes using high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS). Extensive research was carried out in model systems on the impact of potential interfering substances on analyte's signals measured in two types of flame (air-C₂H₂ and N₂O-C₂H₂). Mutual analyte interactions were also taken into account. Different background corrections offered by the HR-CS AAS spectrometer were tested to obtain interference-free analyte signals and the best detectability. Using an air-C₂H₂ flame and 1 % La solution as a spectrochemical buffer provided good sensitivity and accurate determinations of Rh, Pd, and Pt using a simple calibration graph. Microwave-assisted leaching of PGE from waste samples with aqua regia at 240 °C for 60 min efficiently leached all target metals, which significantly simplified and shortened the sample preparation step. The detectability of the method (detection limit of 0.4, 0.6, and 5 mg kg^-1 for Rh, Pd, and Pt, respectively) and precision (< 7 %) were satisfactory. The accuracy of the method was confirmed by analysis of certified reference materials (spent automotive catalyst (ERM-EB504), electronic scrap (BAM-M505a)), and calculated zeta score values. The recoveries for Rh, Pd, and Pt in ERM-EB504 were 93, 101, and 96 %, respectively, and for Pd in BAM-M505a, 97 %. The developed method can be used to assess the value of secondary raw materials, such as various types of spent catalysts and e-waste containing Rh, Pd, and Pt.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126894"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing mass spectrometry interpretability by ComDim-ICA multi-block analysis: Geographical and varietal traceability of Brazilian Coffea canephora.","authors":"Michel Rocha Baqueta, Juliana Azevedo Lima Pallone, Eduardo Jorge Pilau, Bruno Henrique Fermino Goulart, Rodrigo Barros Rocha, Patrícia Valderrama, Douglas N Rutledge","doi":"10.1016/j.talanta.2024.126927","DOIUrl":"10.1016/j.talanta.2024.126927","url":null,"abstract":"<p><p>Mass spectrometry can gain analytical interpretability by studying complementarity and synergy between the data obtained by the same technique. To explore its potential in an untargeted metabolomic application, the objective of this work was to obtain organic and aqueous coffee extracts of three coffee Canephora groups produced in Brazil with distinctive aspects: geographical origin and botanical variety. Aqueous and organic extracts of roasted coffee beans were analyzed by direct infusion electrospray ionization mass spectrometry. Due to the large number of samples, the injector of the liquid chromatography system was used to automate the analysis. The column was removed, and a peak tube was added to connect the system directly to the mass spectrometer to inject both polar and nonpolar fractions of the coffee extracts individually. The technique provided characteristic fingerprinting mass spectra that not only allowed for differentiation of geographical origins but also between robusta and conilon botanical varieties. The mass spectra of the organic and water extracts represented two separate data blocks to be analyzed by the ComDim-ICA multi-block data analysis method. While the classical ComDim is based on applying PCA to the iteratively reweighted concatenated matrices, in the ComDim-ICA, the factorization is done using independent components analysis, which promotes specific improvements since it is based on extracting components that are statistically independent of one another. The results highlighted by ComDim-ICA show that both water and organic extracts contributed with important ions to the characterization of the coffee composition. However, the results revealed a high variability of metabolomic composition within each botanical variety (Robusta Amazônico and Conilon Capixaba) and geographical provenance (Rondônia indigenous-1, Rondônia non-indigenous-2 and Espírito Santo-3). Even so, water mass spectra differentiated the botanical variety Conilon from Robusta based on significant ions related to trigonelline, caffeic acid, caffeoylquinic acid, and methylpyridinium; both water and organic mass spectra differentiated Rondônia indigenous from Rondônia non-indigenous and Espírito Santo Conilon based on significant ions related to benzoic acid, pentose, coumaric acid, caffeine in the organic extract and malonic acid, pentose, caffeoylquinic acid, methyl pyridinium, caffeine, and sucrose present in the water extract. With the proposed approach acquiring ion fingerprints of different coffee extracts and their subsequent analysis by ComDim-ICA, new complementary chemical aspects of Brazilian Coffea canephora were put in evidence.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126927"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}