IF 5.6 1区化学

Talanta Pub Date : 2025-05-02 DOI: 10.1016/j.talanta.2025.128257

Junlan Yu , Wenjie Li , Jiachen Zhao , Guijie Hao , Zhe Feng , Fangkai Han , Jiahua Wang , Xiaodan Liu , Fuwei Pi , Huang Dai , Xin Liu , Yafang Shen

{"title":"A MOF-818-based nanozyme-linked aptasensor for microcystin-LR detection in edible algae powder","authors":"Junlan Yu , Wenjie Li , Jiachen Zhao , Guijie Hao , Zhe Feng , Fangkai Han , Jiahua Wang , Xiaodan Liu , Fuwei Pi , Huang Dai , Xin Liu , Yafang Shen","doi":"10.1016/j.talanta.2025.128257","DOIUrl":"10.1016/j.talanta.2025.128257","url":null,"abstract":"<div><div>The development of simple, rapid, and sensitive detection methods for microcystin-leucine-arginine (MC-LR) is crucial for ensuring food safety. A novel nanozyme-linked colorimetric aptasensor was developed using metal-organic framework (MOF) material MOF-818. The aptamer and MOF-818 with complementary DNA (cDNA) on its surface (MOF-818/cDNA) served as bioreceptors and signal reporters, respectively. Without MC-LR, MOF-818/cDNA bound to the aptamer, catalyzing the oxidation of 3,5-Di-tert-butylcatechol (3,5-DTBC) and producing yellow-colored products. While in the presence of MC-LR, it bound to the aptamers competitively, inhibited MOF-818/cDNA capture and reduced catalytic activity, enabling colorimetric quantification. The method achieved a linear detection range of 1–200 ng/mL and a limit of detection of 0.849 ng/mL. It successfully quantified MC-LR in spiked lake water and tap water, as well as MC-LR-contaminated edible algae (Spirulina platensis and Chlorella pyrenoidosa) powders, with recovery rates ranging from 81.45 % to 136.67 %. This research provided a cost-effective, efficient and portable method for MC-LR screening in food and environmental samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"Article 128257"},"PeriodicalIF":5.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput microanalysis of steel segregation using picosecond laser-induced breakdown spectroscopy 皮秒激光诱导击穿光谱法对钢偏析的高通量微分析

IF 5.6 1区化学

Talanta Pub Date : 2025-05-02 DOI: 10.1016/j.talanta.2025.128229

Shoujie Li , Zixiong Qin , Yuan Lu , Lihui Ren , Jingjun Lin , Zhenzhen Wang , Yoshihiro Deguchi , Ronger Zheng

引用次数: 0

Analysis of volatile organic compounds (VOCs) in e-cigarettes: A thermal desorption coupled with gas chromatography-mass spectrometry (TD-GC-MS) approach 电子烟中挥发性有机化合物（VOCs）的分析：热脱附-气相色谱-质谱（TD-GC-MS）方法

IF 5.6 1区化学

Talanta Pub Date : 2025-05-02 DOI: 10.1016/j.talanta.2025.128255

Eduard F. Valenzuela , Ivana Ferreira Simões , Fernanda Warken Rosa Camelier , Aníbal de Freitas Santos Júnior , Zenilde das Graças Guimarães Viola , Giselle Carolina da Fonseca Andrade

{"title":"Analysis of volatile organic compounds (VOCs) in e-cigarettes: A thermal desorption coupled with gas chromatography-mass spectrometry (TD-GC-MS) approach","authors":"Eduard F. Valenzuela , Ivana Ferreira Simões , Fernanda Warken Rosa Camelier , Aníbal de Freitas Santos Júnior , Zenilde das Graças Guimarães Viola , Giselle Carolina da Fonseca Andrade","doi":"10.1016/j.talanta.2025.128255","DOIUrl":"10.1016/j.talanta.2025.128255","url":null,"abstract":"<div><div>This study investigates 67 volatile organic compounds (VOCs) in exhaled e-cigarette aerosol using thermal desorption coupled with gas chromatography-mass spectrometry (TD-GC-MS). As e-cigarettes become increasingly popular as an alternative to traditional smoking, it is essential to understand the chemical composition of their emitted aerosol and their potential health and environmental impacts. The methods employed were optimized and validated, demonstrating high accuracy, excellent linearity, and robust detection limits. The analytical range extended from 0.5 to 25 ng in tube, with detection limits between 0.08 and 0.33 ng in tube and quantification limits from 0.12 to 0.50 ng in tube. Linearity was confirmed with R2 values surpassing 0.9900 for calibration curves. Recovery rates ranged from 88 % to 109 %, and coefficients of variation were between 2 % and 13 %. Twenty-one e-liquid samples were obtained from liquor stores in Belo Horizonte, Brazil, featuring fruity flavors (e.g., watermelon, apple, mint, vanilla, strawberry, pineapple, mango) and three brands. A total of 28 VOCs were identified and quantified, including regulated substances such as BTEX (0.55–9.50 ng in tube or 0.28–4.75 ng mL−1), acetone (0.55–0.86 ng in tube or 0.28–0.43 ng mL−1), and Chlorinated organic pollutants. The TD-GC-MS method proves effective for VOCs analysis in e-cigarettes, providing valuable insights for future research and regulatory efforts.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128255"},"PeriodicalIF":5.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MALDI-TOF mass spectrometric immunoassay of multiple tumor biomarkers for non-small cell lung cancer screening. 多种肿瘤生物标志物的MALDI-TOF质谱免疫分析用于非小细胞肺癌筛查。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-06 DOI: 10.1016/j.talanta.2025.127550

Mengchen Wang, Chengxiang Zhu, Nan Feng, Yiran Li, Jiahui Sun, Huangxian Ju

{"title":"MALDI-TOF mass spectrometric immunoassay of multiple tumor biomarkers for non-small cell lung cancer screening.","authors":"Mengchen Wang, Chengxiang Zhu, Nan Feng, Yiran Li, Jiahui Sun, Huangxian Ju","doi":"10.1016/j.talanta.2025.127550","DOIUrl":"10.1016/j.talanta.2025.127550","url":null,"abstract":"Cancer biomarkers have been facing some issues such as poor accuracy and low sensitivity in the early diagnosis of tumors. Utilizing biotin-labelled peptide as a mass tag (MT), this work proposes a high-throughput biosensing strategy for matrix-assisted laser desorption/ionization-time of flight mass spectrometric (MALDI-TOF-MS) immunoassay of multiple lung cancer biomarkers. Due to little required dosage, satisfied stability, high sensitivity and accuracy, this method can achieve off-site centralized signal detection after on-site sample incubation. The proposed approach has been successfully applied for the detection of carcinoembryonic antigen (CEA), carbohydrate antigen199 (CA199), carbohydrate antigen 125 (CA125) and cytokeratin-19-fragment (CY211) in serum samples from various stages of non-small cell lung cancer. Based on the analysis of multiple parameters and pathological results, significant differences in biomarkers are found in serum samples of lung cancer patients at different stages. More importantly, the analysis of multiple tumor biomarkers can improve the accuracy and sensitivity of early diagnosis. Therefore, the multiple immunoassay based on MALDI-TOF MS exhibits exceptional performance in terms of high throughput, little sample dosage, stability and sensitivity.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127550"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Bright luminescent Zn2GeO4:Mn NP/MXene hydrogel-based ECL biosensor for glioblastoma diagnosis" [Talanta 276 (2024) 126214]. “用于胶质母细胞瘤诊断的明亮发光Zn2GeO4:Mn NP/MXene水凝胶型ECL生物传感器”的勘误表[Talanta 276(2024) 126214]。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-02-02 DOI: 10.1016/j.talanta.2025.127628

Shuanglin Deng, Wenyan Li, Zhenrun Li, Peilin Wang, Qiang Ma

引用次数: 0

Fluorescent distinguishing flavonoid glycosides against aglycones based on the selective recognization of boric acid-functional Eu(III)-organic framework. 基于硼酸-功能Eu(III)-有机骨架选择性识别的黄酮类苷与苷元的荧光识别。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-07 DOI: 10.1016/j.talanta.2025.127559

Shuyun Zhu, Kunming Sun, Shuyi Liu, Qian Wang, Jiatong Fan, Xian-En Zhao, Guobao Xu

{"title":"Fluorescent distinguishing flavonoid glycosides against aglycones based on the selective recognization of boric acid-functional Eu(III)-organic framework.","authors":"Shuyun Zhu, Kunming Sun, Shuyi Liu, Qian Wang, Jiatong Fan, Xian-En Zhao, Guobao Xu","doi":"10.1016/j.talanta.2025.127559","DOIUrl":"10.1016/j.talanta.2025.127559","url":null,"abstract":"Flavonoid glycosides are formed by dehydration condensation of aglycones and sugar molecules. Therefore, discrimination of flavonoid glycosides from their corresponding aglycones is a challenging task because they contain the same aglycone part in their molecular structures. Herein, boric acid-functional Eu(III)-organic framework (BA-Eu-MOF) was applied to discriminate flavonoid glycosides including baicalin (Bai), wogonoside (Wog), rutin (Rut), puerarin (Pue), quercitrin (Que) and astragalin (Ast) from their corresponding aglycones for the first time. Besides as organic ligand to sensitize the luminescence of Eu3+ through \"antenna\" effect, 5-boronobenzene-1,3 dicarboxylic acid provided recognition site for flavonoid glycosides. Infrared, fluorescence, UV-vis, and mass spectra were used to investigate the recognition reaction between BA-Eu-MOF and flavonoid glycosides. The data indicated that the cis-diols of flavonoid glycosides from sugars covalently bonded to boric acid group to form cyclic boronic esters, which quenched the fluorescence of BA-Eu-MOF at 620 nm through decreasing the intersystem efficiency, inner filter effect and photoelectron transfer. In contrast, aglycones could not alter the fluorescence of BA-Eu-MOF because of no covalent bond between them. This probe exhibited high sensitivity towards flavonoid glycosides with the low detection limits of 3.3 nM, 3.5 nM, 33 nM, 56 nM, 5.1 nM and 5.5 nM for Bai, Que, Wog, Ast, Pue and Rut, respectively. The unique recognition ability of boric acid group enables selective and sensitive detection of flavonoid glycosides without the interference of their corresponding aglycones.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127559"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to 'A dual-targeted electrochemical aptasensor for neuroblastoma-related microRNAs detection' [Talanta, Volume 280, (2024) 126772]. “用于神经母细胞瘤相关microrna检测的双靶向电化学感应传感器”的勘误[Talanta，卷280，（2024）126772]。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-10 DOI: 10.1016/j.talanta.2025.127523

Gabriel Bk Sasa, Baixun He, Chong Chen, Zetao Chen, Shuang Li, Cherie S Tan

引用次数: 0

Ultrasensitive photoelectrochemical detection of cancer markers based on heterojunctions constructed from Bi₂O₃ star-like flower nanoclusters and CdS hollow nanorods. 基于Bi2O3星形花纳米团簇和CdS空心纳米棒异质结的超灵敏光电化学检测癌症标志物。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-09 DOI: 10.1016/j.talanta.2025.127563

Zimeng Wang, Jiawei Wang, Jingchun Ou, Xianbiao Hu, Minglun Wei, Zinan Chen, Rongshan Lin, Jianying Yang, Wenhua Gao

{"title":"Ultrasensitive photoelectrochemical detection of cancer markers based on heterojunctions constructed from Bi2O3 star-like flower nanoclusters and CdS hollow nanorods.","authors":"Zimeng Wang, Jiawei Wang, Jingchun Ou, Xianbiao Hu, Minglun Wei, Zinan Chen, Rongshan Lin, Jianying Yang, Wenhua Gao","doi":"10.1016/j.talanta.2025.127563","DOIUrl":"10.1016/j.talanta.2025.127563","url":null,"abstract":"CYFRA21-1 is a tumor marker for lung cancer, and its rapid and accurate detection can provide evidence for the early diagnosis of lung cancer. In this work, Bi-Fe turnbull blue analogues (Bi-Fe-TBA) were synthesized by the self-templating method. Bi2O3-SFNs was prepared by simple oxidation in air using Bi-Fe-TBA as a template. Bi2O3 Star-like Flower Nanoclusters (Bi2O3-SFNs) and CdS Hollow Nanorods (CdS-HNRs) were used to form a unique type II heterojunction for the first time. The arrangement of energy levels between CdS-HNRs and Bi2O3-SFNs, along with their hollow structure and star shape, effectively suppressed the recombination of photogenerated electrons and holes while shortening carrier transport distance. An ultra-sensitive PEC biosensor was developed to detect the lung cancer marker CYFRA21-1, leveraging the superior photoelectric conversion capabilities of Bi2O3-SFNs/CdS-HNRs. The sensor demonstrates outstanding stability, specificity, reproducibility as well as a wide linear range (10-4 - 10 ng mL-1) and low detection limit (4.23 × 10-5 ng mL-1). This study is valuable for the preparation of other functional materials using TBA as a template.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127563"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a cpGFP-based biosensor for enhanced quantification of glycolate production in Escherichia coli. 设计一种基于cpgfp的生物传感器，用于增强大肠杆菌中乙醇酸生产的定量。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-13 DOI: 10.1016/j.talanta.2025.127529

Qiwei Wang, Zhifen Huang, Sen Ma, Mingxue Ma, Sheng Ye, Si Liu

{"title":"Engineering a cpGFP-based biosensor for enhanced quantification of glycolate production in Escherichia coli.","authors":"Qiwei Wang, Zhifen Huang, Sen Ma, Mingxue Ma, Sheng Ye, Si Liu","doi":"10.1016/j.talanta.2025.127529","DOIUrl":"10.1016/j.talanta.2025.127529","url":null,"abstract":"The growing demand for glycolate, fueled by economic development, requires the advancement of production methods. Escherichia coli (E. coli), a preferred host for glycolate production, has undergone extensive metabolic engineering to improve yield. Developing rapid and precise methods for quantifying glycolate concentration is essential for screening high-yielding strains. Here, we present the engineering of a novel circularly permuted green fluorescent protein (cpGFP)-based glycolate sensor, termed GLYCO. GLYCO exhibits high specificity (minimal interference from other metabolites), stability (no decrease in performance after 15 days at -80 °C), and ease of detection via fluorescence measurement, enabling effective in vitro glycolate quantification. GLYCO spans a quantification range from 10 μM to 1 mM, facilitating effective monitoring of glycolate production in metabolically engineered E. coli strains. This biosensor represents a significant advancement in the metabolic engineering toolkit, facilitating efficient detection and optimization of glycolate production in E. coli, with potential applications in industrial biotechnology.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127529"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel method for detecting genetic biomarkers in blood-based liquid biopsies using surface plasmon resonance imaging and magnetic beads shows promise in cancer diagnosis and monitoring. 一种利用表面等离子体共振成像和磁珠在血液液体活检中检测遗传生物标志物的新方法在癌症诊断和监测中显示出前景。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-07 DOI: 10.1016/j.talanta.2025.127543

Noemi Bellassai, Roberta D'Agata, Elena Giordani, Giovanna Ziccheddu, Roberto Corradini, Giuseppe Spoto

{"title":"A novel method for detecting genetic biomarkers in blood-based liquid biopsies using surface plasmon resonance imaging and magnetic beads shows promise in cancer diagnosis and monitoring.","authors":"Noemi Bellassai, Roberta D'Agata, Elena Giordani, Giovanna Ziccheddu, Roberto Corradini, Giuseppe Spoto","doi":"10.1016/j.talanta.2025.127543","DOIUrl":"10.1016/j.talanta.2025.127543","url":null,"abstract":"Directly detecting biomarkers in liquid biopsy for diagnosis and personalized treatment plays a crucial role in managing cancer relapse and increasing survival rates. Typically, the standard analysis of circulating tumour DNA requires lengthy isolation, extraction, and amplification steps, leading to sample contamination, longer turnaround time and higher assay costs. Surface plasmon resonance is an emerging and promising technology for rapid and real-time dynamic biomarker monitoring in liquid biopsy. Here, we propose a new SPR imaging biosensing approach to detect tumour DNA circulating in the blood of colorectal cancer patients by exploiting the unique properties of superparamagnetic particles. Micrometer beads functionalized with a biotinylated oligonucleotide can directly capture DNA target sequences bearing single-nucleotide variations of KRAS oncogene in human blood plasma. Mutated and wild-type peptide nucleic acid probes immobilized on an SPR gold surface recognize complementary and non-complementary DNA targets by discriminating a single nucleotide mismatch. The new assay allows for detecting p.G13D mutated DNA in buffer and spiked human plasma at attomolar level (down to 300 copies mL-1) with minimal sample manipulation and in just a few microliters. The assay was validated using plasma samples from colorectal cancer patients and healthy donors, by discriminating mutated DNA circulating in patients and wild-type DNA found in healthy blood donors. This feature underscores the potential of the liquid biopsy assay as a valuable tool for the diagnosis and monitoring of cancer.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127543"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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