Talanta最新文献

{"title":"Automated sample preparation systems for food analysis: A mini-review","authors":"Qiong Xue ,&nbsp;Chang Liu ,&nbsp;Xiao-Hua Zhou ,&nbsp;Dezhao Kong ,&nbsp;Jinghui Zhang ,&nbsp;Wei Shen ,&nbsp;Sheng Tang","doi":"10.1016/j.talanta.2026.129395","DOIUrl":"10.1016/j.talanta.2026.129395","url":null,"abstract":"<div><div>Sample preparation has emerged as an essential component in complex food matrix analysis. Automated systems integrate precise machine operation, cutting-edge sample extraction methodologies and sophisticated detection equipment, and have been proven to effectively improve the accuracy and detection efficiency in food analysis. This review provides a mini-overview of automated sample preparation methods currently employed in the field of food analysis. These primarily encompass automated solid-phase extraction methods, microfluidics, robotic systems and integration with detection equipment. In addition, the main challenges currently faced by automated sample preparation were discussed, and suggestions for future research directions were also proposed. This review aims to serve as a reference for researchers in the field and facilitate their work on automated sample preparation methods for food analysis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129395"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced 2-butanone sensing properties of CeO2/WO3 heterojunction with multilevel channels inherited from pollen biotemplate","authors":"Chuanxuan Zhou ,&nbsp;Shaowei Li ,&nbsp;Ziyi Zhang ,&nbsp;Dousheng Han ,&nbsp;Fuchao Yang","doi":"10.1016/j.talanta.2026.129436","DOIUrl":"10.1016/j.talanta.2026.129436","url":null,"abstract":"<div><div>The ultimate optimization of natural systems in terms of “structure-function-environment adaptation” can provide inspiration for the design of advanced materials. Considering the urgent demand for efficient detection of 2-butanone in industrial safety and environmental monitoring, and the limitations of existing WO₃-based gas sensors including low response intensity and poor selectivity, this research focuses on developing modified WO₃-based composite materials with enhanced gas sensing performance using natural biological templates. In this work, an immersion process combined with calcination technology was employed to successfully replace the biosensitive scaffolds on pollen grains with gas-sensitive CeO₂/WO₃ composites. This material exhibits outstanding response to 2-butanone at 185 °C, significantly surpassing the undoped sample while maintaining high selectivity. The outstanding sensing performance is attributed to its unique hierarchical structure and the synergistic effect of the CeO₂/WO₃ heterostructure. This study provides a new perspective for us to explore low-cost gas sensors with natural biological morphologies and opens up new possibilities for expanding the applications of WO₃-based composites.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129436"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146028063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ HF-etched MXene as a novel electrode modifier for sensitive detection of bisphenol A","authors":"Ana Agurto ,&nbsp;María Pilar da Silva ,&nbsp;Luis Vázquez ,&nbsp;María Dolores Petit-Domínguez ,&nbsp;Elena Casero ,&nbsp;Carmen Quintana ,&nbsp;Elias Blanco","doi":"10.1016/j.talanta.2026.129363","DOIUrl":"10.1016/j.talanta.2026.129363","url":null,"abstract":"<div><div>This study explores the use of MXene, a relatively recent class of 2D materials, as an innovative modifier of glassy carbon (GC) electrodes for the electrochemical detection of bisphenol A (BPA), a compound of significant environmental and health concern. MXene Ti₃C₂ was synthesized via in-situ hydrofluoric acid generation using a NaF/HCl mixture that is considered a milder synthetic route in comparison with ex-situ hydrofluoric acid, and safer because it avoids the handling and storage of commercial HF. Nanomaterials obtained at various stages of MXene synthesis were morphologically characterized using scanning electron microscopy. With respect to the bare GC, MXene improved the BPA electroanalysis due to the known MXene properties such as metallic conductivity. This enhanced response was further improved when MXene was exfoliated via a delamination process. Scan rate studies proved that the BPA mechanism was governed by a diffusion-controlled process. Once MXene concentration in aqueous suspensions used to modify the GC surface was optimized, pH and differential pulse parameters were adjusted. The optimal conditions were 0.1 M phosphate buffer at pH 7 as electrolyte, pulse amplitude of 60 mV and scan rate of 20 mV/s. Sensor performance evaluation yielded a detection limit of 0.11 μM. The applicability of the sensor was supported by the analysis of real water samples. Results were corroborated by ultra-high performance liquid chromatographic analysis, demonstrating the sensor's applicability and reliability.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129363"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-acetylcysteine-functionalised a multimodal HPLC stationary phase for broad-range separations","authors":"Hayriye Aral ,&nbsp;Tarık Aral ,&nbsp;Murat Sunkur ,&nbsp;Mehmet Çolak ,&nbsp;Mesut Bağcık","doi":"10.1016/j.talanta.2026.129397","DOIUrl":"10.1016/j.talanta.2026.129397","url":null,"abstract":"<div><div>In this study, a new multimodal HPLC stationary phase derived from N-acetyl-<span>l</span>-cysteine was synthesized and comprehensively evaluated across a broad polarity spectrum. In chromatographic stationary phase design, two fundamental criteria are particularly important: a facile, low-cost synthesis and broad applicability across diverse analyte classes. The present stationary phase fulfils both requirements, as it is prepared from a commercially available starting material via a simple single-step immobilisation onto silica, providing a practical and economical synthetic route alongside wide analytical utility. The molecular architecture of the ligand, incorporating a pH-responsive carboxyl group, a hydrogen-bond-donating and -accepting amide moiety, a polarisation-sensitive sulfur atom, and hydrophobic methyl and methylene units, creates a versatile interaction environment capable of mediating hydrophobic, polar, dispersive, and weak cation-exchange processes. This multifunctional design enables the stationary phase to adapt its selectivity according to analyte structure and mobile-phase conditions, supporting both reversed-phase-like and HILIC-like retention behaviour within a single chromatographic platform. The chromatographic performance of the NAC-derived stationary phase was systematically evaluated using a wide range of chemically and biologically relevant analytes. Under HILIC conditions, the column successfully resolved 7 of the 9 highly polar nucleobases and nucleosides. In reversed-phase mode, compounds with high hydrophobicity—including 6 alkyl benzenes, 4 polycyclic aromatic hydrocarbons, 6 Sudan dyes, 8 anilines, and 7 benzoic acid derivatives—were efficiently separated. Under the same reversed-phase conditions, the stationary phase successfully resolved 10 analytes from a twelve-analyte plant growth regulator mixture and 9 sulphonamides. In addition, a 6 herbicide was also effectively separated, highlighting the multimodal selectivity of the stationary phase. Retention trends were interpreted using analyte logD and pKa values, clarifying the combined contributions of hydrophobic, polar, and weak ion-exchange interactions to overall selectivity. The N-acetyl-<span>l</span>-cysteine-based stationary phase provides a finely balanced interaction profile, enabling high separation performance for analytes spanning from extreme polarity to pronounced hydrophobicity and demonstrating broad analytical utility.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129397"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-oxidation and proximity crosslinking of G-quadruplex in G-quadruplex /hemin peroxidase system","authors":"Xiaojuan Yang ,&nbsp;Jin Zhou ,&nbsp;Dihua Shangguan","doi":"10.1016/j.talanta.2025.129344","DOIUrl":"10.1016/j.talanta.2025.129344","url":null,"abstract":"<div><div>G-quadruplex (G4)/hemin peroxidase has emerged as a robust platform for signal amplification in biosensors. While offering distinct advantages over protein enzymes, its susceptibility to inactivation often results in low apparent catalytic activity, thereby limiting broader applications. To address this, we investigated the side reactions occurring during the catalytic process of G4/hemin DNAzyme in both N-acetyl-cysteine (NAC) and H₂O₂ systems. Notably, high-molecular-weight products were observed when the hemin/G4 ratio exceeded 10. Further analysis revealed that these products primarily resulted from the self-oxidation of G4 sequences or proximity cross-linking with hemin fractions, substrates, and coexisting DNA sequences. Our findings provide critical insights into the inactivation mechanism and offer guidance for enhancing the catalytic efficiency of G4-based biosensors.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129344"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel nanomaterial-based SERS sensors for antibiotic detection: Design strategies and enhancement mechanisms","authors":"Rui Yang ,&nbsp;Jiaqi Hou ,&nbsp;Yingnan Quan ,&nbsp;Tian Liang ,&nbsp;Xiangbin Kong ,&nbsp;Bin Yang ,&nbsp;Yujie Yu ,&nbsp;Panpan Wang ,&nbsp;Mingxiao Li","doi":"10.1016/j.talanta.2025.129335","DOIUrl":"10.1016/j.talanta.2025.129335","url":null,"abstract":"<div><div>Rapid detection and accurate quantification of antibiotic residues in the environment are essential for reducing associated health risks. Surface-enhanced Raman spectroscopy (SERS) amplifies Raman signals of target molecules through nanostructured substrates, and combined with its unique molecular fingerprint recognition capability, enables highly sensitive and rapid detection of trace levels of antibiotics in environmental samples. This paper introduces the principles and detection mechanisms of SERS technology and reviews recent advances in the application of SERS sensors based on semiconductors, graphene, organic frameworks, and aptamers for antibiotic detection. We further outline the construction methods and performance characteristics of these SERS sensors, analyze the advantages and limitations of different nanostructured substrates in antibiotic detection, and discuss future research directions and trends in SERS-based sensing platforms. Overall, this review provides comprehensive insights into the use of SERS with diverse nanostructured substrates for antibiotic detection, offering valuable reference points for environmental antibiotic monitoring.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129335"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-channel microfluidic PEC aptasensor driven by S-scheme heterojunction BiOBr@CdIn2S4 for integrated detection of three mycotoxins","authors":"Tiantong Liu ,&nbsp;Jing Zhang ,&nbsp;Xing Liu ,&nbsp;Wenli Jiang ,&nbsp;Yifan Chen ,&nbsp;Jifang Zhang ,&nbsp;Xiaojian Li ,&nbsp;Jinhui Feng","doi":"10.1016/j.talanta.2026.129413","DOIUrl":"10.1016/j.talanta.2026.129413","url":null,"abstract":"<div><div>The co-occurrence of mycotoxins in food represents a significant threat to human health, with aflatoxin B1 (AFB1), fumonisin B1 (FB1), and ochratoxin A (OTA) being among the most hazardous examples. Consequently, the development of reliable, multiplexed, and ultrasensitive methods for their integrated detection is essential for effective food safety monitoring. A three-channel microfluidic aptasensor has been constructed for concurrent determination of AFB1, FB1, and OTA with high sensitivity and specificity. This device incorporates an S-scheme heterojunction photoelectrode based on a metal-organic framework <u>(</u>MOF)-derived BiOBr@CdIn₂S₄ (M-BiOBr@CdIn₂S₄) composite, where CdIn₂S₄ nanosheets are uniformly anchored onto a tubular M-BiOBr framework. This configuration provides abundant sites for aptamer immobilization, promotes directional charge migration and enables efficient charge separation through rational band alignment, resulting in a stable and amplified photocurrent response. Upon toxin binding, the photocurrent decreases in proportion to the logarithm of the toxin concentration, providing a wide dynamic range: 5.0 × 10⁻⁵ to 5.0 × 10¹ ng mL⁻¹ for AFB1, 5.0 × 10⁻³ to 5.0 × 10³ ng mL⁻¹ for FB1, and 5.0 × 10⁻⁴ to 5.0 × 10² ng mL⁻¹ for OTA. The detection limits are as low as 1.7 × 10⁻² pg mL⁻¹, 1.6 pg mL⁻¹, and 1.5 × 10⁻¹ pg mL⁻¹, respectively. The aptasensor demonstrates exceptional reproducibility, specificity, and stability within complex food matrices, thereby establishing a versatile platform for the rapid and ultrasensitive monitoring of multiple mycotoxins, which is crucial for safeguarding food safety and public health.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129413"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-ligand-functionalized gold nanoparticles on ZIF-8: A bimodal ratiometric sensor for sensitive detection of penicillin G","authors":"Yi Ding,&nbsp;Zhiyuan Xu,&nbsp;Tianxia Chen,&nbsp;Mengxia Huang,&nbsp;Weiwei Li,&nbsp;Wenxin Ji,&nbsp;Xiangyu Liu","doi":"10.1016/j.talanta.2026.129383","DOIUrl":"10.1016/j.talanta.2026.129383","url":null,"abstract":"<div><div>Metal-organic frameworks (MOFs) provide a unique platform for tailoring the optical microenvironment of metal nanoparticles. However, simultaneously achieving high brightness, dual-emission self-calibration, and target-specific recognition remains challenging. In this sense, we prepare a dual-ligand engineered gold nanoparticle (Au NPs) system exhibiting well-resolved bimodal fluorescence. The Au NPs can be integrated with ZIF-8 via surface loading and in-situ encapsulation, respectively, yielding two kinds of composites. Comparative studies reveal that, compared to the in-situ encapsulated material (Au NPs@ZIF-8), the surface-loaded architecture (Au NPs/ZIF-8) demonstrates significantly higher quantum yield and photostability, thus enabling an optimal scaffold for sensing applications. Leveraging this, a ratiometric fluorescent probe is developed to linearly quantify penicillin G down to 0.8 nM in real samples with recoveries of 86–108 %. Notably, the dual-emission ratiometric is able to read-out mitigates matrix effects without sophisticated sample pretreatment, indicating robustness against turbidity and autofluorescence. This work proposes a new approach to fabricate high-performance ratiometric fluorescence sensors for sensitive detection of penicillin G in food and environment.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129383"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-immobilization of DNAzyme and horseradish peroxidase via biomineralization for electrochemical sensing of antibiotics","authors":"Tai Ye ,&nbsp;Shuying Yue ,&nbsp;Long Bai ,&nbsp;Haohao Chen ,&nbsp;Chenyang Li ,&nbsp;Rehemutula Wubuli ,&nbsp;Kehua Dang ,&nbsp;Min Yuan ,&nbsp;Hui Cao ,&nbsp;Ziqing Ye ,&nbsp;Fei Xu","doi":"10.1016/j.talanta.2026.129418","DOIUrl":"10.1016/j.talanta.2026.129418","url":null,"abstract":"<div><div>Co-immobilization of horseradish peroxidase (HRP) with G-quadruplex/hemin complex often suffer from low efficiency, intricate procedures, and reduced catalytic performance. To address these challenges, we developed a biomineralization strategy that integrates HRP and G-quadruplex/hemin into the single nanosheet structure. By engineering a split aptamer sequence into a G-rich domain, the nanosheets synergistically combine enhanced catalytic activity with better target-specific molecular recognition. Compared with either free HRP or it biomineralized counterpart alone, the prepared nanosheets exhibited markedly improved peroxidase-like activity. In the presence of enrofloxacin, specific aptamer-target binding facilitated the formation of a ternary complex on the electrode surface, enabling the catalysis of H₂O₂-mediated oxidation of TMB. Under the optimized conditions, the electrochemical aptasensor displayed a wide linear detection range of 1 nM–10 μM with a low detection limit of 0.53 nM. Beyond high selectivity, the sensor demonstrated excellent practical applicability in spiked milk and egg white samples. The generality of the developed platform was further confirmed by its successful extension to other antibiotics detection through replacing the split-aptamer pairs.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129418"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid phase microextraction for urinary VOC analysis using portable GC-MS: Method development and validation against benchtop instrumentation","authors":"Mark Woollam ,&nbsp;Serenidy Eckerle ,&nbsp;Eray Schulz ,&nbsp;Sara Button ,&nbsp;Mangilal Agarwal","doi":"10.1016/j.talanta.2025.129329","DOIUrl":"10.1016/j.talanta.2025.129329","url":null,"abstract":"<div><div>Biological volatile organic compounds (VOCs) are expressed in noninvasive sample types such as urine and have emerged as promising biomarkers for disease detection. Gas chromatography-mass spectrometry (GC-MS) is the gold standard for VOC analysis, providing robust separation, quantification, and identification capabilities. While highly precise and accurate, benchtop GC-MS instruments require a large physical footprint and trained personnel for operation and data interpretation. Advancements in portable GC-MS technology have enabled point-of-care VOC analysis outside traditional laboratory settings, though most systems are designed for environmental and hazardous material detection. Therefore, the current study sought to develop a new method which is optimized to detect biological VOCs in urine headspace through solid phase microextraction (SPME) coupled with portable GC-MS. Method development included optimization of sample preparation, on-column, and MS parameters to improve sensitivity, repeatability, and chromatographic resolution. The SPME-based method was compared to another sampling method using the standard air probe, which demonstrated VOCs were enriched in SPME with an average log₂ fold change equal to 3.5. Intra- and inter-day repeatability was benchmarked through analysis of UTAK urine standards, which showed that VOCs generally displayed relative standard deviation (RSD) values below 25 %. Finally, samples from three healthy volunteers and the UTAK standard (n = 3 samples each) were analyzed using the portable GC-MS. This showed that the portable system could discern unique VOC trends between the volunteers. Compound identities and quantitative signal patterns were independently confirmed using a benchtop GC-MS, thus bringing confidence in the accuracy of the portable platform.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129329"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}