Talanta最新文献

{"title":"Evaluating the greenness of MP-AES spectroscopy through AGREE metrics","authors":"Luca Vignali ,&nbsp;Elena Bernardi ,&nbsp;Enrico Sassoni ,&nbsp;Martina Cappelletti ,&nbsp;Elena Cofini ,&nbsp;Daniele Cespi","doi":"10.1016/j.talanta.2026.129536","DOIUrl":"10.1016/j.talanta.2026.129536","url":null,"abstract":"<div><div>The principles of green chemistry have progressively expanded into green analytical chemistry (GAC) and green sample preparation (GSP), fostering the development of quantitative tools such as AGREE and AGREEprep to assess the environmental sustainability of analytical workflows. Although these tools are increasingly adopted, their application is often limited to numerical scoring, with scarce discussion of the underlying assumptions and decision-making processes.</div><div>This study critically evaluates the applicability and effectiveness of AGREE and AGREEprep in the context of Microwave-induced Plasma Atomic Emission Spectroscopy (MP-AES) analyses, using a real analytical workflow adopted within the Italian PRIN2022 project SECURE-COATS focused on developing functionalized and sustainable coatings for the conservation of stone materials in cultural heritage. The case study involves the determination of Ag, Zn, Ca and P in aqueous solutions obtained from immersion tests in bacterial growth media and from artificial ageing tests, aimed at assessing, respectively, the release of potentially antibacterial metals and the durability of the new coatings.</div><div>A systematic and transparent data collection was performed to populate both AGREE tools, and the rationale behind each input parameter is explicitly discussed. This approach allows a critical assessment of how methodological choices, instrumental features and sample preparation steps influence the final greenness scores.</div><div>This work demonstrates their value as decision-support instruments for improving analytical processes also in the field of chemistry for cultural heritage, by reducing both environmental impact and operational costs. The provided data and proposed methodology could serve as a useful reference framework for future green assessments.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129536"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compound-specific hydrogen isotope analysis of lignin methoxy groups for tea provenance discrimination: Comparison with dual-water equilibration","authors":"Kasun Binduhewa ,&nbsp;B.A.T. Amalka ,&nbsp;Champa K. Dissanayake ,&nbsp;Rohana Chandrajith ,&nbsp;L.S.K. Hettiarachchi ,&nbsp;M.A.B. Ranatunga","doi":"10.1016/j.talanta.2026.129540","DOIUrl":"10.1016/j.talanta.2026.129540","url":null,"abstract":"<div><div>Hydrogen stable isotope compositions in plant tissues encode elevation-dependent climatic signals that can be exploited for the authentication of geographic origin. However, bulk hydrogen isotope measurements conflate exchangeable and non-exchangeable hydrogen pools, obscuring the climatic signal. Compound-specific analysis of lignin methoxy groups targets a chemically uniform, non-exchangeable hydrogen pool that preserves source-water isotopic signals. In this study, compound-specific gas chromatography–isotope ratio mass spectrometry (GC–IRMS) analysis of lignin methoxy <em>δ</em>²H was directly compared with non-exchangeable <em>δ</em>²H values derived independently using the dual-water-vapour equilibration method to evaluate methodological agreement and robustness. This approach was applied to 45 Sri Lankan orthodox black tea samples spanning low-grown, mid-grown, and high-grown elevation zones. Methoxy <em>δ</em>²H values decreased systematically with elevation, ranging from −123 ± 3‰ (low-grown) to −150 ± 2‰ (high-grown), yielding a 27‰ gradient across approximately 2000 m. Analysis of variance identified elevation as the dominant source of variation, accounting for 99.5% of the total variance (F = 4202, p &lt; 0.001). Methodological performance was evaluated through direct comparison with non-exchangeable <em>δ</em>²H values obtained by dual-water vapour equilibration, which produced independent <em>δ</em>²H_nex estimates strongly correlated with GC–IRMS methoxy measurements (r = 0.9149, slope = 0.9601). The exchangeable hydrogen fraction remained consistent across all samples (18.49 ± 1%), indicating similar hydrogen-bonding environments at all elevations. Bland–Altman analysis demonstrated predictable inter-method agreement, with a systematic offset of −23‰. The strong proportional agreement, despite a consistent and predictable systematic offset, demonstrates that compound-specific methoxy δ²H analysis yields results consistent with established equilibration-based methods while providing a more direct, chemically defined measurement of non-exchangeable hydrogen. These findings highlight the potential of compound-specific methoxy <em>δ</em>²H analysis as a geochemically robust marker for elevation-based discrimination of Sri Lankan tea and provide a foundation for future multi-laboratory evaluation of isotope-based authentication frameworks.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129540"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a cELISA based on a trivalent nanobody–HRP fusion protein targeting BP26 for brucellosis diagnosis","authors":"Gaowa WuDong ,&nbsp;Qing Lu ,&nbsp;Shurong Yin ,&nbsp;Yong Shi ,&nbsp;Xuan Bu ,&nbsp;Yunyi Zhai ,&nbsp;Xiaofang Liu ,&nbsp;Yuanhao Yang ,&nbsp;Lu Zhang ,&nbsp;Dong Zhou ,&nbsp;Yaping Jin ,&nbsp;Aihua Wang","doi":"10.1016/j.talanta.2026.129497","DOIUrl":"10.1016/j.talanta.2026.129497","url":null,"abstract":"<div><div>Brucellosis is a zoonotic disease that poses a serious threat to both humans and animals, not only creating a significant public health risk but also causing considerable economic losses. Timely and accurate diagnosis is critical for the effective prevention and control of brucellosis. However, current ELISAs target lipopolysaccharides (LPS) and often cross-react with other Gram-negative bacteria, resulting in limited specificity. Moreover, their high cost limits large-scale implementation and routine laboratory screening. In this study, a cELISA targeting the <em>Brucella</em> protein BP26 was developed using a trivalent nanobody–horseradish peroxidase fusion protein (3Nbs-HRP). The assay exhibited excellent performance, with a sensitivity of 99% and a specificity of 95.9%, and demonstrated high repeatability. High concordance was achieved compared with the serum agglutination test (SAT) and a commercial ELISA. In conclusion, these findings suggest that the assay is an efficient and cost-effective serological method suitable for clinical screening in routine diagnostic laboratory settings, with the potential to enhance brucellosis surveillance and control efforts.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129497"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentiometric electrodes for total dissolved solids: Universal ion response from crosslinked polymer membranes","authors":"Ye Zhang ,&nbsp;Yu Cheng ,&nbsp;Dajing Yuan ,&nbsp;Maoguo Li","doi":"10.1016/j.talanta.2026.129532","DOIUrl":"10.1016/j.talanta.2026.129532","url":null,"abstract":"<div><div>Total dissolved solids (TDS) are a critical parameter in water quality assessment. Conductivity measurements are commonly used for TDS estimation but it is easily biased by factors such as temperature and ionic composition. Here, we introduce novel potentiometric all-crosslinked membrane electrodes for direct assessment of TDS in water samples. The ion-sensitive electrodes, fabricated using epoxy-amine and thiol-ene click polymerization, demonstrated high sensitivity to a range of common cations and anions covering a wide concentration range (0.1 – 100 mM). Experimental results showed an excellent linear correlation between the open circuit potential of the electrodes and logarithmic TDS in fresh water samples. The proposed membrane electrodes offered several advantages including lower cost, ease of operation, and rapid response. We believe that potentiometry based on the electrodes could become an alternative to traditional conductivity-based approaches, providing convenient and reliable TDS measurements in various water matrices.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129532"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-functional molecularly imprinted polymers for the selective removal and Ultrasensitive FAPA-MS detection of picloram and diclofenac in environmental waters","authors":"Tomasz Nazim,&nbsp;Michał Cegłowski","doi":"10.1016/j.talanta.2026.129528","DOIUrl":"10.1016/j.talanta.2026.129528","url":null,"abstract":"<div><div>This study introduces molecularly imprinted polymers (MIPs) based on a polyethyleneimine (PEI) backbone for their selective removal and detection. To enhance molecular recognition, the PEI was pre-functionalized with 3,4-dichlorophenyl isocyanate (3,4-DCPI) at varying degrees (0-30%) before cross-linking. This significantly improved adsorption, with the most functionalized MIPs achieving high maximum capacities of 466 mg g⁻¹ for picloram and 282 mg g⁻¹ for diclofenac, significantly outperforming conventional methacrylic-based MIPs prepared for comparison. Adsorption kinetics followed the pseudo-first-order model, and isotherms fit the Langmuir model; the process was found to be spontaneous and exothermic. The MIPs demonstrated excellent selectivity, achieving selectivity factors of up to 6.14 for picloram and 9.22 for diclofenac against challenging structural analogs (including ibuprofen and naproxen), and maintained high performance with only ∼13% reduction in capacity over five adsorption-desorption cycles. When coupled with flowing atmospheric-pressure afterglow mass spectrometry (FAPA-MS) as a solid-phase extraction medium, the MIPs lowered the limits of detection to 0.03 μmol dm⁻³ (picloram) and 0.076 μmol dm⁻³ (diclofenac). The method's robustness was validated in spiked river water and bovine plasma samples, showing excellent accuracy and precision in both environmental and biological matrices, presenting a versatile platform for remediation and sensitive analytical applications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129528"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEGDA hydrogel-based biosensor for continuous salivary cortisol monitoring without pre-treatment","authors":"Suyoung Lee ,&nbsp;Eunseo Cho ,&nbsp;Jaeyoon Song ,&nbsp;Neethu Ramakrishnan ,&nbsp;Dong-Sik Shin ,&nbsp;Youngeun Kwon ,&nbsp;Jinsik Kim","doi":"10.1016/j.talanta.2026.129518","DOIUrl":"10.1016/j.talanta.2026.129518","url":null,"abstract":"<div><div>Cortisol is a steroid hormone that has wide-ranging effects on the body, including roles in mental health, immune response, and metabolic regulation, and it is directly linked to various disorders. However, its use in point-of-care settings has been limited, mainly because cortisol levels fluctuate constantly in the body and traditional biofluids like blood or saliva usually need extensive pre-treatment before analysis. Here, we introduce a poly(ethylene glycol) diacrylate (PEGDA) hydrogel-based biosensor to address these challenges. PEGDA hydrogels form polymer networks with mesh structures, and by adjusting the mesh size, interfering substances in samples, such as mucin in saliva, can be automatically filtered by size. To enable detection in the nanomolar range, we established a signal amplification system by incorporating cortisol antibodies and cortisol-HRP into the hydrogel and ensuring their stable retention. This allows for quantitative detection of cortisol in both artificial and real saliva across physiologically relevant concentration ranges. Additionally, continuous monitoring of salivary cortisol was achieved, and by applying time-series multidimensional analysis, the biosensor demonstrated the ability to accurately track dynamic cortisol levels in the body. Through the development of this hydrogel-based biosensor, we anticipate advances in disease diagnosis, long-term monitoring, and personalized health management systems.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129518"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Site-specific immobilization of tyrosinase on magnetic polystyrene nanospheres as dual-functional biosensor for enhanced colorimetric analysis of atrazine in vegetables","authors":"Xiaochu Chen ,&nbsp;Minxing Huang ,&nbsp;Jianbin Chen ,&nbsp;Xiaopeng Wang ,&nbsp;Qiaoyun Cheng ,&nbsp;Zhaohua Ping ,&nbsp;Guihua Wang ,&nbsp;Yufeng Gao","doi":"10.1016/j.talanta.2026.129522","DOIUrl":"10.1016/j.talanta.2026.129522","url":null,"abstract":"<div><div>The enzyme-based colorimetric sensor allows rapid and visual detection of food contaminants but is limited by low sensitivity and impurity interference. In the present work, a colorimetric biosensor for atrazine detection was fabricated through oriented immobilization of tyrosinase onto magnetic polystyrene nanospheres (MPSNPs). MPSNPs carriers were prepared by surface amination of monodisperse polystyrene nanospheres, followed by concanavalin A modification to introduce specific affinity sites for glycosyl groups on tyrosinase. Then, tyrosinase was bound to these specific sites via affinity self-assembly. The obtained MPSNPs immobilized tyrosinase (MPSNP-ConA/Tyr) were characterized and displayed uniform and stable structure, excellent enzyme activity, and good adsorption properties for the target atrazine. The MPSNP-ConA/Tyr, combined with 4-aminoantipyrine/catechol colorimetric indicator and smartphone, was used to analyze atrazine in three vegetable samples. The method enabled atrazine enrichment, enzyme activity inhibition reaction, and quantitative analysis in a single platform. This MPSNP-ConA/Tyr based method exhibited good linearity in the range of 0.1-2.0 mg L⁻¹ (γ &gt; 0.9953) with a limit of detection (LOD) of 0.054 mg L⁻¹. The accuracy evaluated by recoveries ranged from 73.1% to 100.5% with relative standard deviations (RSDs) of 1.2-12.5%. A comparison with the GC-MS/MS method was performed using real vegetable samples (cucumber, carrot, and lettuce), and comparable atrazine concentrations were obtained, validating the practical applicability of the proposed method.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129522"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural tuning of bridging linkers on M13 phages for high-affinity detection of enterovirus A71","authors":"Yun Bok Lee,&nbsp;Hyo Jeong Yang,&nbsp;Jae Hwan Shin,&nbsp;Kumarasamy Jayakumar,&nbsp;Jong Pil Park","doi":"10.1016/j.talanta.2026.129516","DOIUrl":"10.1016/j.talanta.2026.129516","url":null,"abstract":"<div><div>Enterovirus A71 (EV−A71), the principal etiological agent of hand, foot, and mouth disease, poses a serious public health concern because infection can induce severe and potentially fatal neurological complications through direct invasion and inflammation of the central nervous system. Current detection methods lack rapid and sensitive analytical capabilities, particularly in the context of wastewater surveillance. This study reports a PEG4-biotin-functionalized M13 bacteriophage engineered as a molecular receptor for the colorimetric detection of EV−A71. VP1-specific phage ligands were identified via phage display and comprehensively characterized using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and transmission electron microscopy (TEM). The optimized biosensor demonstrated strong linearity for VP1 (0.03−3.91 nM, R² = 0.969) and intact virions (10²−10⁶ PFU/mL, R² = 0.974), achieving detection limits of 0.22 nM and ∼10² PFU/mL, respectively. These findings underscore the potential of the PEG4-biotinylated M13 platform for rapid, sensitive, and on-site enterovirus monitoring in public health surveillance systems.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129516"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Memoriam Jaromir (Jarda) Ruzicka: Impressions about a world-renowned scientist.","authors":"E A G Zagatto","doi":"10.1016/j.talanta.2026.130172","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130172","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130172"},"PeriodicalIF":6.7,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A near-infrared fluorescent probe with rapid-response hypochlorite for imaging rheumatoid arthritis and wound healing in vivo","authors":"Zhengyang Shi ,&nbsp;Hanbin Wu ,&nbsp;Zhengxin Chen ,&nbsp;Yao Wang ,&nbsp;Long Zhang ,&nbsp;Zhongxi Huang ,&nbsp;Zhipeng Zhang ,&nbsp;Haiyun Yin ,&nbsp;Yusheng Xie ,&nbsp;Changmin Yu","doi":"10.1016/j.talanta.2026.129485","DOIUrl":"10.1016/j.talanta.2026.129485","url":null,"abstract":"<div><div>Real-time monitoring of hypochlorite (ClO⁻) fluctuations in inflamed tissues is crucial for evaluating the onset, progression, and prognosis of inflammatory diseases such as rheumatoid arthritis (RA). In this study, we rationally developed a small-molecule fluorescent probe (<strong>SX-ClO</strong>) based on a malononitrile–isophorone fluorophore skeleton coupled with a diaminomaleonitrile moiety as the ClO⁻-responsive unit. The probe operated through an intramolecular ICT modulation mechanism upon specific reaction with ClO⁻, enabling a remarkable fluorescence turn-on response with high selectivity, excellent sensitivity, and minimal background interference. <strong>SX-ClO</strong> demonstrated a reliable ability to visualize both endogenous and exogenous ClO⁻ in live RAW 264.7 macrophages, thereby facilitating dynamic evaluation of intracellular oxidative microenvironments. Beyond <em>in vitro</em> performance, <strong>SX-ClO</strong> achieved high-contrast fluorescence imaging of ClO⁻ accumulation in λ-carrageenan–induced RA mouse joints, effectively distinguishing inflamed joints from healthy ones. Moreover, the probe successfully detected elevated ClO⁻ levels in MRSA-infected wound tissues, providing clear visualization of bacterial infection–associated oxidative bursts. Overall, these properties enable real-time visualization and dynamic monitoring of pathological processes at the molecular level, highlighting its potential for clinical translation in early diagnosis and disease progression tracking.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"303 ","pages":"Article 129485"},"PeriodicalIF":6.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146090288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}