IF 5.6 1区化学

Talanta Pub Date : 2024-09-19 DOI: 10.1016/j.talanta.2024.126920

Zan Gong, Panpan Yuan, Yuqing Gan, Xi Long, Zhiwei Deng, Yalan Tang, Yanjing Yang, Shian Zhong

{"title":"A one-pot isothermal Fluorogenic Mango II arrays-based assay for label-free detection of miRNA.","authors":"Zan Gong, Panpan Yuan, Yuqing Gan, Xi Long, Zhiwei Deng, Yalan Tang, Yanjing Yang, Shian Zhong","doi":"10.1016/j.talanta.2024.126920","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126920","url":null,"abstract":"The capability to detect a small number of miRNAs in clinical samples with simplicity, selectivity, and sensitivity is immensely valuable, yet it remains a daunting task. Here, we described a novel Mango II aptamers-based sensor for the one-pot, sensitive and specific detection of miRNAs. Target miRNA-initiated mediated catalyzed hairpin assembly (CHA) would allow for the production of plenty of DNA duplexes and the formation of the complete T7 promoter, motivating the rolling circle transcription (RCT). Then, the subsequent RCT process efficiently generates a huge number of repeating RNA Mango II aptamers, brightened by the incorporation of fluorescent dye TO1-B for miRNA quantification, realizing label-free and high signal-to-background ratio. Moreover, this assay possesses a remarkable ability to confer high selectivity, enabling the distinction of miRNAs among family members with mere 1- or 2- nucleotide (nt) differences. By employing the proposed assay, we have successfully achieved a sensitive evaluation of miR-21 content in diverse cell lines and clinical serum samples. This offers a versatile approach for the sensitive assay of miRNA biomarkers in molecular diagnosis.","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au³⁺-etching of gold nanobipyramids and CIELab color space. 基于乙酰胆碱酯酶介导的纸基 Au3+-蚀刻金纳米双锥体和 CIELab 色彩空间的有机磷农药现场定量检测纸基多色传感器。

IF 5.6 1区化学

Talanta Pub Date : 2024-09-19 DOI: 10.1016/j.talanta.2024.126925

Feng Zhang, Yu Gao, Enxi Ren, Ling Fang, Weijuan Yang, Liaoyuan Zhang, Zongwen Wang

{"title":"Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space.","authors":"Feng Zhang, Yu Gao, Enxi Ren, Ling Fang, Weijuan Yang, Liaoyuan Zhang, Zongwen Wang","doi":"10.1016/j.talanta.2024.126925","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126925","url":null,"abstract":"On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au3+-etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-deposited nylon membrane embedded within a portable and temperature-controlled paper-based analytical device. This system, coupled with a colorimeter-based quantitative method, enables the development of a practical paper-based multicolor sensor (PMS) for on-site quantitative detection of three common OPs (paraoxon, dichlorvos, and trichlorfon). In the absence of OPs, AChE hydrolyzes acetylthiocholine to thiocholine, which reduces Au3+ to Au+. The presence of OPs inhibits AChE activity, thereby preserving Au3+ to etch AuNBPs on nylon membranes, accompanied by multicolor changes. These color changes can be simply quantified by measuring the a∗ parameter of the CIELab color space using a portable colorimeter. Under optimal conditions, the PMS displayed eight OPs-corresponding color changes with a minimum detectable concentration of 1.0-10 μg/L (visual observation) and limits of detection of 0.8-7.2 μg/L (colorimeter) and 0.2-3.4 μg/L (UV-vis spectrometry). The PMS successfully determined the OPs in vegetable and rice samples with recoveries of 89.0-109 % and RSDs (n = 5) of <6 %. These results were consistent with those obtained using the HPLC-MS method. The PMS demonstrates excellent portability, AuNBPs stability, detection sensitivity, and reproducibility, making it a promising tool for the on-site quantitative detection of OPs residues in food. Furthermore, the paper-based etching system coupled with the colorimeter-based quantitative method provides a valuable reference to develop practical PMSs for various targets in diverse fields.","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target-promoted activation of DNAzyme walker for in situ assembly of hemin/G-quadruplex nanowires enable ultrasensitive and label-free electrochemical myocardial microRNA assay. 靶标促进激活 DNAzyme walker 以原位组装 hemin/G-quadruplex 纳米线，从而实现超灵敏和无标记的电化学心肌 microRNA 检测。

IF 5.6 1区化学

Talanta Pub Date : 2024-09-19 DOI: 10.1016/j.talanta.2024.126923

Tengteng Shan, Lingrong Cui, Huimin Zhang, Kaiqin Li, Jianmei Yang, Yan Zhao, Yun Xiang, Ruo Yuan

{"title":"Target-promoted activation of DNAzyme walker for in situ assembly of hemin/G-quadruplex nanowires enable ultrasensitive and label-free electrochemical myocardial microRNA assay.","authors":"Tengteng Shan, Lingrong Cui, Huimin Zhang, Kaiqin Li, Jianmei Yang, Yan Zhao, Yun Xiang, Ruo Yuan","doi":"10.1016/j.talanta.2024.126923","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126923","url":null,"abstract":"The concentration elevation of myocardial microRNA (miRNA) biomarker is associated with the pathogenic process of acute myocardial infarction (AMI), and sensitive quantification of myocardial miRNA biomarker plays an important role for early AMI diagnosis and its treatment. In response, this work describes an ultrasensitive and non-label electrochemical biosensor for the assay of myocardial miRNA based on cascade signal amplifications integrated by DNAzyme walker and hemin/G-quadruplex nanowires. The DNAzyme walker is activated by presence of target miRNAs to move along the electrode surface to cyclically cleave the substrate hairpins to release G-quadruplex segments, which further trigger the in situ formation of many hemin/G-quadruplex nanowires. The large amounts of hemin intercalated into the DNA nanowires subsequently generate drastically magnified electrochemical current signals for highly sensitive label-free assay of myocardial miRNAs down to 15.7 fM within dynamic range of 100 fM to 10 nM. Such a biosensor also has high selectivity and can monitor myocardial miRNAs in diluted serums at low levels, providing a sensitive and reliable platform for diagnosing infarct-associated cardiovascular diseases.","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142306870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocompatible sensors for ammonia gas detection. 用于氨气检测的生物兼容传感器。

IF 5.6 1区化学

Talanta Pub Date : 2024-09-19 DOI: 10.1016/j.talanta.2024.126916

Maria Angustias Torres-Molina, Miguel M Erenas, Mariano Ortega Munoz, Luis Fermin Capitan Vallvey, Isabel M Perez de Vargas Sansalvador

引用次数: 0

Rapid and accurate identification of Gastrodia elata Blume species based on FTIR and NIR spectroscopy combined with chemometric methods. 基于傅立叶变换红外光谱和近红外光谱以及化学计量学方法，快速准确地鉴定 Gastrodia elata Blume 的种类。

IF 5.6 1区化学

Talanta Pub Date : 2024-09-19 DOI: 10.1016/j.talanta.2024.126910

Guangyao Li, Jieqing Li, Honggao Liu, Yuanzhong Wang

{"title":"Rapid and accurate identification of Gastrodia elata Blume species based on FTIR and NIR spectroscopy combined with chemometric methods.","authors":"Guangyao Li, Jieqing Li, Honggao Liu, Yuanzhong Wang","doi":"10.1016/j.talanta.2024.126910","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126910","url":null,"abstract":"Different varieties of Gastrodia elata Blume (G. elata Bl.) have different qualities and different contents of active ingredients, such as polysaccharide and gastrodin, and it is generally believed that the higher the active ingredients, the better the quality of G. elata Bl. and the stronger the medicinal effects. Therefore, effective identification of G. elata Bl. species is crucial and has important theoretical and practical significance. In this study, first unsupervised PCA and t-SNE are established for data visualisation, follow by traditional machine learning (PLS-DA, OPLS-DA and SVM) models and deep learning (ResNet) models were established based on the fourier transform infrared (FTIR) and near infrared (NIR) spectra data of three G. elata Bl. species. The results show that PLS-DA, OPLS-DA and SVM models require complex preprocessing of spectral data to build stable and reliable models. Compared with traditional machine learning models, ResNet models do not require complex spectral preprocessing, and the training and test sets of ResNet models built based on raw NIR and low-level data fusion (FTIR + NIR) spectra reach 100 % accuracy, the external validation set based on low-level data fusion reaches 100 % accuracy, and the external validation set based on NIR has only one sample classification error and no overfitting.","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic and metabolomic analysis of plasma for pain at different labor stages 不同产程疼痛血浆的蛋白质组和代谢组分析

IF 5.6 1区化学

Talanta Pub Date : 2024-09-19 DOI: 10.1016/j.talanta.2024.126905

引用次数: 0

Highly efficient and broad-spectrum antibacterial carbon dots combat antibiotic resistance. 高效、广谱的抗菌碳点可对抗抗生素耐药性。

IF 5.6 1区化学

Talanta Pub Date : 2024-09-19 DOI: 10.1016/j.talanta.2024.126926

Huimin Miao, Panyong Wang, Jie Wu, Xinlu Li, Yuwei Du, Haiyang Yan, Qiannan You, Wenfei Dong, Li Li

{"title":"Highly efficient and broad-spectrum antibacterial carbon dots combat antibiotic resistance.","authors":"Huimin Miao, Panyong Wang, Jie Wu, Xinlu Li, Yuwei Du, Haiyang Yan, Qiannan You, Wenfei Dong, Li Li","doi":"10.1016/j.talanta.2024.126926","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126926","url":null,"abstract":"Bacterial infections have become a major global public health issue, particularly with the emergence of multidrug-resistant strains. Therefore, developing non-antibiotic antimicrobial agents is crucial for treating drug-resistant bacterial infections. Building on previous research into natural products as novel antibacterial agents, this study synthesized curcumin-derived carbon dots using curcumin and ethylenediamine as raw materials through a hydrothermal method. The resulting carbon dots not only improved the water solubility and stability of curcumin but also exhibited highly efficient broad-spectrum antibacterial activity. Detailed investigations into the antibacterial performance and mechanisms of the carbon dots were conducted through experiments such as minimum inhibitory concentration (MIC) determination, live/dead bacterial staining, morphological studies, nucleic acid concentration detection, and reactive oxygen species (ROS) detection. The results indicated that the carbon dots significantly damaged the structural integrity of bacteria and generated large amounts of ROS. They exhibited remarkable antibacterial effects against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, and effectively inhibited drug-resistant MRSA. Their antibacterial efficacy was notably superior to that of broad-spectrum antibiotics such as chloramphenicol and Sulfadiazine. This study highlights the potential application of curcumin-derived carbon dots in combating bacterial infections and provides valuable insights for developing novel antibacterial agents derived from natural products.","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A smartphone-based multichannel magnetoelastic immunosensor for acute aortic dissection supplementary diagnosis. 用于急性主动脉夹层辅助诊断的基于智能手机的多通道磁弹性免疫传感器。

IF 5.6 1区化学

Talanta Pub Date : 2024-09-18 DOI: 10.1016/j.talanta.2024.126915

Yunmei Cao, Qiannan Wang, Mengshu Han, Yunxuan Zhang, Zhongyun Yuan, Kai Zhuo, Hongpeng Zhang, Zhijin Xing, Hu Jin, Chun Zhao

{"title":"A smartphone-based multichannel magnetoelastic immunosensor for acute aortic dissection supplementary diagnosis.","authors":"Yunmei Cao, Qiannan Wang, Mengshu Han, Yunxuan Zhang, Zhongyun Yuan, Kai Zhuo, Hongpeng Zhang, Zhijin Xing, Hu Jin, Chun Zhao","doi":"10.1016/j.talanta.2024.126915","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126915","url":null,"abstract":"Some biomarkers of acute aortic dissection (AAD) can be used for the potential supplementary diagnosis of AAD, such as C-reactive protein (CRP), smooth muscle myosin heavy chain (SmMHC), and D-dimer (D-D). However, the current measurement methods for common markers primarily rely on sophisticated instruments. The operation process is complicated, and the reagents used are expensive. To provide chronic disease monitoring and home self-examination services for potential AAD patients in real time, we developed a smartphone-based multichannel magnetoelastic (ME) immunosensing device to detect protein levels. Our immunosensor reduced the aforementioned restrictions and demonstrated excellent performance for the supplementary diagnosis of AAD. In this paper, we successfully combined the intelligent terminal with the hardware system to sample the resonance frequency shift (RFS) on the multichannel ME immunosensor. According to the target detection objects with their respective antibodies in the immune binding response, multiple experiments were conducted to detect multiple groups of samples, and we found that a CRP concentration, a SmMHC concentration, and a D-D concentration in the range of 0.1-100μg/mL, 1-4ng/mL, and 0.25-5μg/mL were linearly proportional to the RFS of the ME immunosensor, respectively. For CRP, SmMHC, and D-D, the sensitivities were 13.37Hz/μg∙mL-1, 155.19Hz/ng∙mL-1, and 332.72Hz/μg∙mL-1, respectively, and the detection limits were 2.634×10-3μg/mL, 1.155×10-2ng/mL, and 3.687×10-3μg/mL, respectively. The experiments demonstrated that the accuracy and stability of our device were comparable to those of the vector network analyzer (VNA, Calibration instrument).","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Achieving precise dual detection: One-tube reverse transcription-recombinase aided amplification (RT-RAA) combined with lateral flow strip (LFS) assay for RNA and DNA target genes from pepper mild mottle virus and Colletotrichum species in crude plant samples. 实现精确的双重检测：单管反转录-重组酶辅助扩增（RT-RAA）结合侧流条带（LFS）检测植物粗样品中辣椒轻度斑驳病病毒和壳斗孢菌的 RNA 和 DNA 目标基因。

IF 5.6 1区化学

Talanta Pub Date : 2024-09-18 DOI: 10.1016/j.talanta.2024.126908

Yuhao Cao, Dankan Yan, Huijie Zhou, Kelei Han, Qionglian Wan, Jiejun Peng, Hongying Zheng, Lin Lin, Fei Yan, Xuemei Song

{"title":"Achieving precise dual detection: One-tube reverse transcription-recombinase aided amplification (RT-RAA) combined with lateral flow strip (LFS) assay for RNA and DNA target genes from pepper mild mottle virus and Colletotrichum species in crude plant samples.","authors":"Yuhao Cao, Dankan Yan, Huijie Zhou, Kelei Han, Qionglian Wan, Jiejun Peng, Hongying Zheng, Lin Lin, Fei Yan, Xuemei Song","doi":"10.1016/j.talanta.2024.126908","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126908","url":null,"abstract":"Ensuring the detection sensitivity of both RNA-derived and DNA-derived target genes in a single reaction has posed a significant challenge for on-site detection of plant pathogens. This challenge was addressed by developing a one-tube dual RT-RAA assay combined with LFS for the rapid on-site detection of pepper mild mottle virus (PMMoV) and four Colletotrichum species causing anthracnose in Solanaceous crops. By testing four different combinations of primer groups, two combinations were precisely adjusted within the dual RT-RAA system to balance amplification efficiency and maintain consistent levels of amplification in crude plant samples. Utilizing commercially accessible small-scale equipment and following a streamlined optimization strategy, the assay achieved a limit of detection of 0.32 copies/μL of target genes in the reaction. Importantly, it demonstrated no cross-reactivity with other plant pathogens, thereby affirming the high sensitivity and specificity of the developed dual RT-RAA-LFS detection assay. Moreover, the entire process took only 25 min from sample collection to the visible presentation of results. The assay was validated with 60 field samples and 10 seed samples, producing results consistent with reverse transcription quantitative polymerase chain reaction (RT-qPCR). Notably, it successfully detected PMMoV in systemic leaves without visible symptoms three days post-inoculation, underscoring its effectiveness in early disease detection. This streamlined strategy offers a valuable approach for rapid, low-cost, and highly sensitive on-site simultaneous detection of RNA genome-contained PMMoV and DNA genome-contained Colletotrichum species.","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of hapten and monoclonal antibody of hesperetin and establishment of enzyme-linked immunosorbent assay. 制备七叶皂苷及单克隆抗体并建立酶联免疫吸附试验。

IF 5.6 1区化学

Talanta Pub Date : 2024-09-18 DOI: 10.1016/j.talanta.2024.126912

Yifan Liu, Zihui Jin, Di Sun, Bo Xu, Tianyu Lan, Qiyang Zhao, Yue He, Jing Li, Yongliang Cui, Yaohai Zhang

{"title":"Preparation of hapten and monoclonal antibody of hesperetin and establishment of enzyme-linked immunosorbent assay.","authors":"Yifan Liu, Zihui Jin, Di Sun, Bo Xu, Tianyu Lan, Qiyang Zhao, Yue He, Jing Li, Yongliang Cui, Yaohai Zhang","doi":"10.1016/j.talanta.2024.126912","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126912","url":null,"abstract":"Hesperetin is the aglycone of hesperidin and is widely found in the Rutaceae plants and herbal medicines. It exhibits antioxidant, detoxifying, anti-inflammatory, and antimicrobial properties, similar to hesperidin. It has also shown potential in the regulation of certain diseases. In order to detect hesperetin in complex matrix samples such as citrus and herbal, we developed an anti-hesperetin monoclonal antibody and established an indirect competitive enzyme-linked immunosorbent assay (icELISA). The half maximal inhibitory concentration (IC50) was determined to be 2.03 ng/mL, the detection range was 0.39-12.73 ng/mL. In practical sample testing, the concentration of hesperidin measured by icELISA is consistent with the result of UPLC-MS/MS, and the correlation coefficient (R2) is 0.97. These results showed that the established method has good accuracy, reproducibility and broad application prospects, and can be used for the detection of hesperetin in complex matrix samples (such as citrus and herbal samples).","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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