Talanta最新文献

{"title":"A novel system for precise identification and explainability analysis based on multimodal learning combining laser-induced breakdown spectroscopy and laser-induced plasma acoustic signals.","authors":"Wenhan Gao, Boyuan Han, Zhuoyi Sun, Yihui Yan, Yanpeng Ye, Jun Feng, Yuyao Cai, Asiri Iroshan, Yuzhu Liu","doi":"10.1016/j.talanta.2025.128182","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128182","url":null,"abstract":"<p><p>This study presents an innovative approach to identify copper types using Laser-Induced Breakdown Spectroscopy (LIBS) in conjunction with Laser-Induced Plasma Acoustic (LIPA) signals. Traditionally overlooked, plasma acoustic signals can indeed provide valuable insights into plasma characteristics essential for copper identification. This study pioneers a cross-modal learning technique, integrating LIBS and LIPA signals, and employs a Support Vector Machine (SVM) for classification. To enhance feature extraction, Principal Component Analysis (PCA) reduces data dimensionality, while SHapley Additive exPlanations (SHAP) assess feature contributions, aiding feature selection. The combined model demonstrates high identification accuracy, and the interpretability analysis deepens our understanding of feature roles in copper detection. This framework not only boosts LIBS-based identification accuracy but also advances the theoretical foundation for multi-modal data fusion in material analysis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128182"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of total- and water-soluble chloride in concrete using an optical sensor.","authors":"Karl L Sterz, Bernhard Müller, Marlene Sakoparnig, Isabel Galan, Andreas Steinegger, Cyrill Grengg, Florian Mittermayr, Sergey M Borisov, Torsten Mayr","doi":"10.1016/j.talanta.2025.128124","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128124","url":null,"abstract":"<p><p>An optical chloride-sensitive film was prepared and utilized to measure the chloride content in powdered concrete samples. The sensor is composed of N,N'-dimethyl-9,9'-biacridiniumnitrat (Lucigenin), a phosphorescent ruthenium complex in dense silica microparticles and a custom-made poly(acrylonitrile-co-acrylamide) matrix, allowing utilization of the dual lifetime referencing (DLR) technique. No dependency on pH value was observed at pH values from 1.0 to 7.0. Among the relevant anions, the sensor did not show any cross-talk to sulphate, but the response towards chloride was reduced in presence of nitrate and acetate ions. The sensor remains stable in water and 50 % acetic acid, which enables measurements of water-soluble chloride and total chloride. The sensor was challenged with concrete samples of various origins and with strong differences in chloride contamination (0.02-0.38 %w/w). The results of the optical measurements are in excellent agreement with results obtained from potentiometric titrations. The divergent binder chemistry of the selected concrete samples and the reliability of the measured results suggest wide applicability of the sensor. The sensor also shows potential to be suitable for field measurements.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128124"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoengineered hybrid hydrogel-based 3D nanocomposite as an antifouling coating interface for enhanced electrochemical aptasensing of estradiol.","authors":"Gopi Karuppaiah, Aneesh Koyappayil, Sachin Ganpat Chavan, Anna Go, Hwang Seowoo, Pooja Ramrao Rathod, SangWook Lee, Min-Ho Lee","doi":"10.1016/j.talanta.2025.128105","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128105","url":null,"abstract":"<p><p>Electrochemical biosensing has emerged as a promising method for the point-of-care detection of various biomarkers. However, its clinical application faces significant challenges due to biofouling when exposed to clinical samples. This study presents a novel nanocomposite coating aimed at addressing biofouling in electrochemical biosensors by developing an Electrochemical Estradiol Aptasensor (EAS). We developed a nanoengineered, hybrid hydrogel-based 3D antifouling nanocomposite interface (ANcI) by crosslinking carboxymethyl chitosan with sodium carboxymethyl cellulose and incorporating highly conductive Ti₃C₂T_x MXene nanostructures. The Aptasensor was constructed on a screen-printed carbon electrode by applying the ANcI as an antifouling layer, followed by the deposition of a gold nanoparticle support layer modified with estradiol-specific aptamers, which serves as the biorecognition element. We evaluated the antifouling capabilities by comparing the performance of the Aptasensor with and without the ANcI when exposed to human serum and bovine serum albumin. This innovative nanocomposite coating offers excellent antifouling properties along with a highly porous structure and electrical conductivity, which are essential for maintaining sensor performance in complex clinical samples. By addressing the limitations of existing antifouling materials, this approach paves the way for the commercialization of electrochemical biosensors with enhanced accuracy and sensitivity. The sensors demonstrate a clinically relevant concentration range of 0.1 pg/mL to 1000 pg/mL, with a limit of detection of 0.127 pg/mL. These findings highlight the potential of this innovative approach to improve electrochemical biosensing across various applications and significantly impact biomarker detection in clinical samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128105"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphitic carbon nitride/1-pyrenebutyric acid N-hydroxysuccinimide/polythiophene nanocomposite photoelectrochemical biosensor for CA 19-9 detection.","authors":"Adriel Yan Sheng Tan, Faliang Cheng, Min Zhang, Michelle T T Tan, Sivakumar Manickam, Kasturi Muthoosamy","doi":"10.1016/j.talanta.2025.128065","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128065","url":null,"abstract":"<p><p>A photoelectrochemical (PEC) biosensor composed entirely of carbon nanomaterials was synthesized to detect carbohydrate antigen 19-9 (CA 19-9). The biosensor platform integrated graphitic carbon nitride (GCN), known for its light sensitivity, polythiophene (PTh), an organic conductive and optically active material, and 1-pyrenebutyric acid N-hydroxysuccinimide (PBASE), which functions both as a biolinker to conjugate CA 19-9 antibody and antigen and as an electron mediator to facilitate electron transfer from GCN to PTh. The formation of a Schottky heterojunction between PTh and GCN reduced the bandgap of GCN from 2.66 to 1.96 eV, which enhanced transfer of photogenerated electrons for cathodic photocurrent generation. The improvement of charge transfer due to heterojunction formation and π-π stacking between GCN and the pyrene group of PBASE is confirmed by cyclic voltammetry (CV), electron impedance spectroscopy (EIS), and chronoamperometry (CA) findings. The highest current of 1.31 μA is observed for combination of 5 wt% PTh with a GCN/PBASE ratio of 1:0.5. Besides evaluating the electron mobility of GCN/PBASE/PTh, CV, EIS, and CA were also used to evaluate the sensor performance. Optimization studies revealed that 0.6 μg of CA 19-9 antibody and 1 h of antigen-antibody immobilization time significantly improved the biosensor response. The GCN/PBASE/PTh biosensor demonstrated high sensitivity to CA 19-9 antigen across a concentration of 50-1000 U/ml and a detection limit as low as 0.052 U/ml. The reported working range is within the limits required for diagnostic testing of patients with hepatic and heart problems as well as for post-treatment monitoring of colorectal and pancreatic cancer patients.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128065"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A smartphone-integrated CuFe aerogel nanozyme for rapid and visual detection of ascorbic acid in real samples.","authors":"Lijun Mao, Wenhua Bi, Jianqi Ye, Xinhao Wan, Yarui Tang, Yao Chen, Wei Liu, Dan Wen","doi":"10.1016/j.talanta.2025.128169","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128169","url":null,"abstract":"<p><p>The development of highly active nanozymes for advanced bioanalysis has attracted considerable attention, yet significant challenges remain. Inspired by the structural features of natural oxidases with transition metal active sites, we designed a bimetallic CuFe aerogel with significantly enhanced oxidase-like activity for the facile and visual detection of ascorbic acid (AA). The incorporation of Fe into the Cu aerogel matrix resulted in a remarkable 7.8-fold increase in oxidase-like activity compared to pure Cu aerogel, as confirmed by comprehensive experimental analyses and theoretical calculations. Leveraging the inhibitory effect of AA on the Cu₆Fe aerogel-catalyzed oxidation reaction, we developed a rapid and sensitive colorimetric detection method, achieving a low detection limit of 0.1 μM. Furthermore, by integrating the Cu₆Fe aerogel with portable test strips and smartphone-based colorimetric analysis, we constructed a visual sensing platform capable of accurate and reliable AA quantification. The practical applicability of this platform was successfully demonstrated through the detection of AA in real-world samples, including fruits and commercial vitamin C beverages. This work not only provides a novel strategy for designing highly active metal aerogel-based nanozymes but also opens new avenues for their application in visual sensing and point-of-care diagnostics.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128169"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mechanism detecting fluoride and tetracycline in food matrices using red-emitting carbon dots.","authors":"Yumin Hao, Xiaole Wen, Xiaofang Zheng, Huiping Wang, Wenjuan Dong, Yang Liu, Shengmei Song, Shaomin Shuang, Yujing Guo, Chuan Dong, Xiaojuan Gong","doi":"10.1016/j.talanta.2025.128126","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128126","url":null,"abstract":"<p><p>Excessive exposure to fluoride and tetracycline can cause severe dental damage, including tetracycline-induced tooth discoloration and dental fluorosis. Herein, we introduce a dual-mechanism sensing strategy using red-emitting carbon dots (R-CDs) for the independent detection of fluoride and tetracycline. A key advantage of R-CDs as sensors is their ability to selectively identify both analytes through long-wavelength emission with a large Stokes shift. For fluoride detection, we developed a fluorescence-enhanced sensor based on R-CDs-Fe³⁺ via a competitive binding mechanism. Meanwhile, tetracycline detection was achieved using a fluorescence-quenching sensor leveraging static quenching and the internal filter effect (IFE). The successful quantification of fluoride and tetracycline in food matrices demonstrates the practical potential of R-CDs in food safety monitoring. Additionally, this study presents a novel framework for designing multi-target detection systems using a single type of carbon dots across different sensing mechanisms.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128126"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-site microfluidic aptasensor for rapid and highly selective detection of microcystin-LR.","authors":"Jihye Lee, Byeolnim Oh, Jaewon Park, Jee Young Kim, Jino Son, Hyun Soo Kim, Yoon-E Choi","doi":"10.1016/j.talanta.2025.128147","DOIUrl":"10.1016/j.talanta.2025.128147","url":null,"abstract":"<p><p>To detect microcystin-LR (MC-LR), a potent cyanotoxin harmful to human health, fast and precise monitoring tools are essential, particularly for on-site applications. In this study, we developed a novel on-site microfluidic aptasensor system that utilizes a fluorescence-tagged aptamer for the rapid and selective detection of MC-LR. By employing a target non-immobilized aptamer selection technique, high-affinity DNA aptamers for MC-LR were identified, and then further optimized through sequence truncation to enhance detection efficiency. The developed aptamer was designed such that its fluorescence is quenched in the absence of MC-LR, but recovered in its presence, enabling a clear signal detection that correlates with a toxin concentration. The developed system achieved a detection limit of 1.9 ppb, significantly lower than the safety threshold suggested by world health organization (WHO) for recreational waters, demonstrating sufficient sensitivity for reliable on-site monitoring. In addition, the microfluidic aptasensor system demonstrated high specificity, exhibiting the strongest response to MC-LR compared to other cyanotoxins. This system is the first portable MC-LR detection tool that allows for on-site environmental monitoring with the advantages of its fast response time and ease of operation. Combining a low detection limit with high accuracy, the microfluidic aptasensor system presents a promising alternative to conventional laboratory-based methods, providing a practical and reliable solution for water quality assessment.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128147"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A triple-helix molecular switch-based fluorescent aptasensor using Klenow fragment-assisted target recycling and Ribonuclease H-powered DNA walker cascade amplification for detection of MRSA.","authors":"Jiaxin Xu, Zidong Zhang, Fangguo Lu, Yi Ning","doi":"10.1016/j.talanta.2025.128131","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128131","url":null,"abstract":"<p><p>MRSA is an antibiotic resistant bacterium that poses a significant threat to the environment and human health due to its bioaccumulation and potential widespread contamination. The prompt and accurate identification of MRSA is essential for enhancing environmental monitoring and clinical management. Here, we develop a triple-helix molecular switch (THMS) fluorescent aptasensor for the determination of MRSA using Klenow fragment (KF)-assisted target recycling and Ribonuclease H (Rnase H)-powered DNA walker cascade amplification. In this method, the target opens the THMS by specifically binding with the aptamer, resulting in the release of target/aptamer complex and DNA walker. KF then initiates the target recycling process via strand-displacement polymerization reaction under the assistance of carboxyfluorescein (FAM)-labeled primer and dNTPs, creating plenty of double-stranded DNA (dsDNA) products. These dsDNA products show low affinity to graphene oxide (GO) and generate strong fluorescence. This fluorescence is considerably significantly amplified in the presence of SYBR Green I (SGI), attributable to the synergistic interaction between dsDNA and SGI. In the interim, Rnase H drives the released DNA walker to automatically walk on the carboxylated graphene oxide surface by cleaving FAM-labeled RNA signal probe (SP), causing the FAMs to dissociate from the carboxylated graphene oxide (CGO). Therefore, fluorescent signal originating from the two reaction pathways can be detected at excitation/emission wavelengths of 480/514 nm. The target measured by this strategy demonstrates a broad linear working range from 10² colony-forming units (CFU)/mL to 10⁷ CFU/mL, with a detection limit (LOD) of 15 CFU/mL. Moreover, this method performs well in milk and pus sample analysis. These results reveal that this aptasensor is highly specific and sensitive for detecting MRSA and is endowed with good potential for food monitoring and clinical diagnosis applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128131"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target-initiated autocatalytic and concatenated DNAzyme/CHA amplification cascades for highly sensitive fluorescent detection of TET1 dioxygenase.","authors":"Daxiu Li, Jie Xiang, Yanni Wang, Yun Xiang, Ruo Yuan","doi":"10.1016/j.talanta.2025.128114","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128114","url":null,"abstract":"<p><p>The sensitive detection of the dysregulated expression of ten-eleven translocation 1 (TET1) dioxygenase, a key DNA 5-methylcytosine (5 mC) oxidation regulator in the expression of developmental genes, is of significant importance for the diagnosis of various genetic diseases and cancers. This study describes the establishment of a highly sensitive fluorescent TET1 bioassay based on the 5 mC-modified/Zn²⁺-dependent DNAzyme-containing hairpin probe and the autocatalytic and concatenated DNAzyme/catalytic hairpin assembly (CHA) signal amplification cascades. TET1 target molecules specifically recognize and cut the 5 mC sites in the hairpin probes to release active DNAzyme sequences, which bind and cleave the double-stem-loop substrate strands to trigger multiple concatenated signal amplification recycling cycles with the presence of the fuel strands and two fluorescently quenched signal hairpins. These DNA reaction cascades thus result in the unfolding of lots of signal hairpins to substantially recover fluorescence for highly sensitive TET1 assay with a calculated detection limit of 6.9 fM. Additionally, such bioassay shows high selectivity toward TET1 and its real applicability has been successfully demonstrated for cancer cell lysate and human serum samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128114"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal triple-mode probe with colorimetric-fluorescence-SERS (CFSERS) for sensitive and quantitative detection of C-reactive protein in clinical diagnostics.","authors":"Wei Li, Xinrui Wang, Mingze Zhu, Xin Huang, Pacifique Hirwa Umutoni, Ting-Hsuan Chen, Jian Lu, Shih-Chi Chen, Guangming Tan, Bryan P Yan, Bee Luan Khoo","doi":"10.1016/j.talanta.2025.128100","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128100","url":null,"abstract":"<p><p>Chronic inflammation remains a major global health concern, necessitating the development of advanced tools for continuous, precise monitoring. This study introduces a novel, clinically impactful triple-mode probe that integrates colorimetric, fluorescence, and surface-enhanced Raman scattering (SERS) signals, offering unparalleled multimodal detection capabilities. The probe's design leverages europium chelate-doped polystyrene nanoparticles (ECNPs), ensuring minimal signal cross-interference through a long Stokes shift. A key innovation is the development of a multimodal mapping algorithm that seamlessly integrates these optical signals, providing a sensitive and robust platform for biomarker detection with broad dynamic ranges. The probe's clinical relevance is demonstrated by its application in lateral flow assays (LFAs) for detecting C-reactive protein (CRP) levels, achieving a detection limit of 6.31 ng/mL and dynamic ranges from 23.13 to 2000 ng/mL, significantly outperforming single-mode detection methods. In clinical validation using urine samples from 31 patients, the triple-mode LFA showed excellent correlation (0.9377) and agreement (93.55 %) with gold standard enzyme-linked immunosorbent assay (ELISA) results. This demonstrates that the proposed multimodal platform offers a highly sensitive, cost-effective, and versatile tool for monitoring inflammation and other disease biomarkers, with substantial potential for clinical applications in diagnostics and disease management.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128100"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}