Talanta最新文献

{"title":"Speciation of selenium-containing small molecules in urine and cell lysate by CE-ICPMS with in-capillary enrichment.","authors":"Yi Lu, Xue Men, Chengxin Wu, Xing Wei, Mingli Chen, Jianhua Wang","doi":"10.1016/j.talanta.2024.126929","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126929","url":null,"abstract":"<p><p>The quantitative speciation of selenium in biological systems is highly important for evaluating health status and elucidating transformations of Se species in physiological and pathological processes. Hyphenation of capillary electrophoresis with inductively coupled plasma mass spectrometry (CE-ICPMS) is promising for this purpose. However, the unfavorable or insufficient sensitivity for selenium analysis with CE-ICPMS seriously limits its practical applications in biological analysis, e.g., cell analysis. Therefore, it is crucial to improve the detection sensitivity for Se species. In this study, CE-ICPMS sensitivities for five selenium species (selenocystamine (SeA), methyl-2-acetamido-2-deoxy-1-seleno-β-d-galactopyranoside (SeSug 1), selenomethionine (SeMet), Se-Methylselenocysteine (MeSeCys) and selenocystine (SeCys)) were improved by in-capillary stacking via pH gradient between the zones of sample-leading buffer and the incorporation of isopropanol. The improvement on sensitivity of up to 9.9 folds was achieved in different biological samples, with LODs of 0.29-0.52 μg L^-1. This approach was further applied for Se speciation in cell lysate, urine and culture medium. It showed that SeMet was more readily reduced in the medium and favorably accumulated by HepG2, HuH-7 and HCCLM3 cells with respect to SeSug 1 and MeSeCys. In cells, all the three Se species were largely transformed into other Se species. Furthermore, more than 70 % of SeMet reduced in medium was transformed into unknown Se species after 48-h interaction with cells.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tunable LDI-MS platform assisted by metal-phenolic network-coated AuNPs for sensitive and customized detection of amino acids.","authors":"Tong Hu, Qi Sang, Dingyitai Liang, Wenjing Zhang, Yuning Wang, Kun Qian","doi":"10.1016/j.talanta.2024.126928","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126928","url":null,"abstract":"<p><p>This study introduces a novel approach for the sensitive and accurate detection of small molecule metabolites, employing metal-phenolic network (MPN) functionalized AuNPs as both adsorbent and matrix to enhance laser desorption/ionization mass spectrometry (LDI-MS) performance. The MPN comprising tannic acid (TA) and transition metal ions (Fe³⁺, Co²⁺, Ni²⁺, Cu²⁺, or Zn²⁺) was coated on the surface of AuNPs, forming metal-TA network-coated AuNPs (M-TA@AuNPs). The M-TA@AuNPs provided a tunable surface for specific interactions with analytes, displaying distinct enrichment efficacies for different amino acids, especially for Cu-TA@AuNPs exhibiting the highest affinity for histidine (His). Under the optimized condition, the proposed method enabled ultrasensitive detection of His, with good linearity (R² = 0.9627) in the low-concentration range (50 nM-1 μM) and a limit of detection (LOD) as low as 0.9 nM. Furthermore, the method was successfully applied to detect His from human urine samples, showcasing its practical applications in clinical diagnostics, particularly in the realm of amino acid-based targeted metabolomics.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical sensor featuring PdFeCo_1-xONPs on carbon paper for the sensitive determination of indole-3-lactic acid levels in serum samples.","authors":"Dieudonne Tanue Nde, Dhananjaya Merum, Gyawali Ghanashyam, Jean Pierre Ndabakuranye, Mohamed A Habila, Arghya Narayan Banerjee, Sang Woo Joo, Theophile Niyitanga, Haekyoung Kim","doi":"10.1016/j.talanta.2024.126919","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126919","url":null,"abstract":"<p><p>A highly sensitive and selective electrochemical sensing platform with self-assembled porous 3-D trimetallic (Pd, Fe, and Co) hybrid anchored on a cost-effective and high-conducting carbon paper (CP) synthesized via a facile and cost-effective hydrothermal impregnation and thermal reduction technique was developed for determining indole-3-lactic acid (ILA) levels in buffer and serum samples. Before the analytical phase, the composite (PdFeCo_1-xONPs@CP electrode) was thoroughly characterized, and different methods were used to investigate the electrochemical properties. The combination of tri-metallics with CP-fibers improved sensing capacities in the linear range of 0.05-30 μM, with sensitivity and limits of detection of and 0.165 ± 0.013 μA/μM and 7.8 ± 0. 2 nM, respectively, towards ILA determination. Furthermore, the developed sensing platform was utilized for the analyses of ILA in sigma, human normal, and alcohol use disorder patients' serum samples. Liquid chromatography in tandem with mass spectrometry was equally used to quantify ILA levels in the serum samples and the results of both methods were compared.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lanthanide MOF-based luminescent sensor array for detection and identification of contaminants in water and biomarkers.","authors":"Wenxi Zhang, Jing Sun, Xiao Li, Sibo Wang, Weitao Zhang, Yaru Gong, Lei Liu, Zhongmin Su","doi":"10.1016/j.talanta.2024.126853","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126853","url":null,"abstract":"<p><p>In today's society, heavy metal ions and antibiotic contaminants have caused great harm to water systems and human health. In this study, six isostructural lanthanide metal-organic frameworks [Ln(H₃imda)₂(TPA)(H₂O)₂](Tb for CUST-881, Eu for CUST-882, Dy for CUST-883, Er for CUST-884, Nd for CUST-885, Sm for CUST-886) were constructed by selecting terephthalic acid (TPA) and 4,5-Imidazoledicarboxylic acid (H₃imda) and lanthanide metal ions via solvethermal method. Among them, CUST-881 and CUST-882 can selectively detect Fe³⁺, Cr₂O₇^2-, CrO₄², and ceftriaxone sodium (CRO) in water systems and uric acid in urine. CUST-881 shows very low detection limits for these five substances. Furthermore, Principal Component Analysis (PCA) was used to distinguish Fe³⁺, Cr₂O₇^2-, CrO₄^2-, and CRO in water. To our knowledge, this is the first time that they have been able to be simultaneously distinguished. In addition, the possible sensing mechanism was studied through UV-visible spectroscopy, Infrared spectroscopy, and PXRD analysis. Furthermore, the probe also showed satisfactory repeatability and recovery when applied to UA samples that simulated urine. Based on the above results, lanthanide metal-organic frameworks have great potential for practical monitoring of contaminants in water environments.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel electrochemiluminescence platform utilizing AuNPs@Uio-66-NH₂ bridged luminescent substrates and aptamers for the detection of pesticide residues in Chinese herbal medicines.","authors":"Chengqiang Li, Haifang Wang, Jiashuai Sun, Peisen Li, Jiwei Dong, Jingcheng Huang, Haowei Dong, Lingjun Geng, Zhiping Yu, Pengwei Zhang, Wei Chen, Yemin Guo, Xia Sun","doi":"10.1016/j.talanta.2024.126924","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126924","url":null,"abstract":"<p><p>A large number of Chinese herbal medicines (CHMs) are included in daily recipes, but their pesticide residues have aroused more and more concerns. In this paper, an electrochemiluminescence aptasensor was constructed for the trace detection of acetamiprid (ACE) in Angelica sinensis and Lycium barbarum. Possessing a large specific surface area, UiO-66 was modified with amino groups to improve biocompatibility, and the addition of AuNPs allowed UiO-66-NH₂ to catalyze the formation of excited states of luminescent molecules (TPrA^⁎; Ru(bpy)₃²⁺^⁎), and AuNPs@UiO-66-NH₂ was used to bridge the aptamer (Au-S) and luminescent substrate (peptide bond). The conventional luminescent reagent Ru(bpy)₃²⁺ was doped with multi-walled carbon nanotubes (MWCNTs) to obtain a more powerful and stable light signal. After optimizing the experimental parameters, the aptasensor could give results in 10 min with a detection range from 1×10^-2-1×10⁴ nM and a lower limit of detection (LOD) of 0.8 pM. The LOD of the study was at least one order of magnitude lower than that of the fluorescence detection method. Furthermore, the accuracy of the aptasensor was validated for spiked recovery experiments.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of 4-n-butylresorcinol by fluorescence derivatization based on dopamine.","authors":"Qing Liu, Si Chen, Yiming Nie, Qian Li, Fang Chen","doi":"10.1016/j.talanta.2024.126909","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126909","url":null,"abstract":"<p><p>4-n-butylresorcinol (4nBR) is a frequently utilized as whitening ingredients in skincare cosmetics. Compared with other whitening ingredients, it can effectively inhibit tyrosinase with lower toxicity and superior inhibition efficacy. Under alkaline conditions, an induced oxidative coupling reaction can occur between 4nBR and dopamine (DA) to generate strong fluorescent substance azamonardine with an intense emission band centering at 476 nm when excited at 440 nm. This phenomenon can be used to establish a fluorescence analysis method for 4nBR. The results indicated that the linear range of the method was 1.0-24.0 nmol L^-1, and the detection limit was as low as 0.25 nmol L^-1. The method showed high sensitivity, good selectivity, mild experimental conditions and low cost. The proposed method was successfully used to detect 4nBR in cosmetics, and the results were consistent with those of HPLC. The spiking recoveries were between 98.2% and 108 %.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical review on metal organic frameworks (MOFs)-based sensors for foodborne pathogenic bacteria detection","authors":"","doi":"10.1016/j.talanta.2024.126918","DOIUrl":"10.1016/j.talanta.2024.126918","url":null,"abstract":"<div><p>The pervasive threat of foodborne pathogenic bacteria necessitates advancements in rapid and reliable detection methods. Traditional approaches suffer from significant limitations including prolonged processing times, limited sensitivity and specificity. This review comprehensively examines the integration of metal organic frameworks (MOFs) with sensor technologies for the enhanced detection of foodborne pathogens. MOFs, with their unique properties such as high porosity, tunable pore sizes, and ease of functionalization, offer new avenues for sensor enhancement. This paper provides a comprehensive analysis of recent developments in MOFs-based sensors, particularly focusing on electrochemical, fluorescence, colorimetric, and surface-enhanced Raman spectroscopy sensors. We have provided a detailed introduction for the operational principles of these sensors, highlighting the role of MOFs play in enhancing their performance. Comparative analyses demonstrate MOFs' superior capabilities in enhancing signal response, reducing response time, and expanding detection limits. This review culminates in presenting MOFs as transformative materials in the detection of foodborne pathogens, paving the way for their broader application in ensuring food safety.</p></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational design of Ag-doped MnFe₂O₄/HNTs for peroxidase-mimicking activity and colorimetric sensing of uric acid in human serum.","authors":"Han Lu, Zhenbo Xiang, Hailong Pang, Qiang Ren","doi":"10.1016/j.talanta.2024.126913","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126913","url":null,"abstract":"<p><p>Mimicking enzyme have significantly advanced sensing assays by replicating native enzyme functions, yet achieving both high catalytic activity and easy recyclability remains a challenge. In this study, Ag-doped MnFe₂O₄/halloysite nanotubes (HNTs) were rationally designed as a novel nanozyme by depositing in-situ Ag and MnFe₂O₄ nanoparticles onto HNTs. The resulting nanocomposite exhibited excellent peroxidase-like activity along with magnetic properties. Leveraging these features, a highly efficient and sensitive colorimetric system for detecting uric acid (UA) was developed. The Ag-doped MnFe₂O₄/HNTs catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H₂O₂, causing a color change from colorless to blue. The system showed a linear absorbance response to UA concentrations ranging from 1 to 20 μM, with a detection limit of 59 nM. Mechanistic studies revealed that reactive oxygen species intermediates (¹O₂) were generated through the decomposition of H₂O₂, leading to peroxidase-like activity in the Ag-doped MnFe₂O₄/HNTs. The assay was successfully applied to detect UA in human serum with recoveries over 99.68 %. This study indicates the successful application of Ag-doped MnFe₂O₄/HNTs for colorimetric UA detection in human serum. This research introduces a novel approach for designing recyclable, high-performance mimicking enzyme and establishes an effective colorimetric sensing platform for UA detection in human serum.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing mass spectrometry interpretability by ComDim-ICA multi-block analysis: Geographical and varietal traceability of Brazilian Coffea canephora.","authors":"Michel Rocha Baqueta, Juliana Azevedo Lima Pallone, Eduardo Jorge Pilau, Bruno Henrique Fermino Goulart, Rodrigo Barros Rocha, Patrícia Valderrama, Douglas N Rutledge","doi":"10.1016/j.talanta.2024.126927","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126927","url":null,"abstract":"<p><p>Mass spectrometry can gain analytical interpretability by studying complementarity and synergy between the data obtained by the same technique. To explore its potential in an untargeted metabolomic application, the objective of this work was to obtain organic and aqueous coffee extracts of three coffee Canephora groups produced in Brazil with distinctive aspects: geographical origin and botanical variety. Aqueous and organic extracts of roasted coffee beans were analyzed by direct infusion electrospray ionization mass spectrometry. Due to the large number of samples, the injector of the liquid chromatography system was used to automate the analysis. The column was removed, and a peak tube was added to connect the system directly to the mass spectrometer to inject both polar and nonpolar fractions of the coffee extracts individually. The technique provided characteristic fingerprinting mass spectra that not only allowed for differentiation of geographical origins but also between robusta and conilon botanical varieties. The mass spectra of the organic and water extracts represented two separate data blocks to be analyzed by the ComDim-ICA multi-block data analysis method. While the classical ComDim is based on applying PCA to the iteratively reweighted concatenated matrices, in the ComDim-ICA, the factorization is done using independent components analysis, which promotes specific improvements since it is based on extracting components that are statistically independent of one another. The results highlighted by ComDim-ICA show that both water and organic extracts contributed with important ions to the characterization of the coffee composition. However, the results revealed a high variability of metabolomic composition within each botanical variety (Robusta Amazônico and Conilon Capixaba) and geographical provenance (Rondônia indigenous-1, Rondônia non-indigenous-2 and Espírito Santo-3). Even so, water mass spectra differentiated the botanical variety Conilon from Robusta based on significant ions related to trigonelline, caffeic acid, caffeoylquinic acid, and methylpyridinium; both water and organic mass spectra differentiated Rondônia indigenous from Rondônia non-indigenous and Espírito Santo Conilon based on significant ions related to benzoic acid, pentose, coumaric acid, caffeine in the organic extract and malonic acid, pentose, caffeoylquinic acid, methyl pyridinium, caffeine, and sucrose present in the water extract. With the proposed approach acquiring ion fingerprints of different coffee extracts and their subsequent analysis by ComDim-ICA, new complementary chemical aspects of Brazilian Coffea canephora were put in evidence.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly active Ti3C2Tx MXene nanoribbons@AuPt bimetallic nanozyme constructed in a “two birds with one stone” manner for colorimetric sensing of dipterex","authors":"","doi":"10.1016/j.talanta.2024.126881","DOIUrl":"10.1016/j.talanta.2024.126881","url":null,"abstract":"<div><div>The bimetallic nanoparticles have received significant attention in the field of colorimetric sensing, while the majority of the previous methods for synthesizing such nanoparticles typically require the use of reducers and involve highly harsh synthesis conditions. In this work, the AuPt bimetallic nanoparticles (AuPtNPs) decorated Ti₃C₂T_xNR nanohybrid (Ti₃C₂T_xNR@AuPt) was firstly synthesized in a “two birds with one stone” manner. Innovatively, during the synthesis process, Ti₃C₂T_xNR served as both reducing agent and supporting agent to prevent aggregation of bimetallic nanoparticles. Due to the good synergistic effect of AuPtNPs and the strong metal-support interaction between AuPtNPs and Ti₃C₂T_xNR, the as-prepared Ti₃C₂T_xNR@AuPt exhibited boosted peroxidase (POD)-like activity. In advantage of the exceptional POD-like activity, a cost-effective and sensitive colorimetric sensing platform was fabricated for dipterex detection with a broad linearity of 1.0 ng mL⁻¹-1.0 μg mL⁻¹ and a low detection limit (0.479 ng mL⁻¹). The detection of dipterex in insecticide samples also yielded favorable recoveries. Moreover, this approach provided a convenient and effective strategy to design and develop novel nanozymes via a “two birds with one stone” strategy.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}