TalantaPub Date : 2025-04-03DOI: 10.1016/j.talanta.2025.128072
Hassan Elzain Hassan Ahmed , Mustafa Soylak
{"title":"A novel nanomotor (ZIF-L(Co)/WSe2 nanocomposite) for nanomotor motion-based dispersive micro-solid phase extraction (NM-d-μSPE) of gold and palladium","authors":"Hassan Elzain Hassan Ahmed , Mustafa Soylak","doi":"10.1016/j.talanta.2025.128072","DOIUrl":"10.1016/j.talanta.2025.128072","url":null,"abstract":"<div><div>A novel method for the separation and enrichment of Au(III) and Pd(II) ions in mining stone and covering samples was developed, using ZIF-L(Co)/WSe<sub>2</sub> as a nanomotor for nanomotor motion-based dispersive micro-solid phase extraction (NM-d-μSPE). The manufactured nanocomposite was characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FE-SEM), and electrochemical analyses. The motion dynamics of the nanomotor have been optimized by modifying the concentration of the fuel (H<sub>2</sub>O<sub>2</sub>) to achieve rapid and efficient movement, which enhanced adsorption performance. Important analytical parameters, such as pH, ligand and sorbent amounts, eluent type and volume, and the influence of matrix ions, were methodically studied. The method exhibited excellent sensitivity, with detection and quantification limits of 0.93 μg L<sup>−1</sup> and 3.10 μg L<sup>−1</sup> for Au(III) and 0.83 μg L<sup>−1</sup> and 2.76 μg L<sup>−1</sup> for Pd(II), respectively. The ZIF-L(Co)/WSe<sub>2</sub> nanomotor showed exceptional adsorption efficiency for the simultaneous extraction of Au(III) and Pd(II) ions in the presence of H<sub>2</sub>O<sub>2</sub> fuel. This method is reliable, fast, and simple, thus offering a useful method for the separation, enrichment, and quantification of Au(III) and Pd(II) in geological samples using flame atomic absorption spectroscopy (FAAS). Additionally, inductively coupled plasma-mass spectrometry (ICP-MS) was used to validate the NM-d-μSPE method.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128072"},"PeriodicalIF":5.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-04-03DOI: 10.1016/j.talanta.2025.128074
Yanbo Zeng , Yixiao Hu , Liyan Zheng , Jianguo Tang , Ganpeng Li , Chunqiong Wang , Mingpeng Liu
{"title":"Synthesis of cuprous iodide coordination polymers using pyridine carbohydrazide as ligands and their application in drug detection","authors":"Yanbo Zeng , Yixiao Hu , Liyan Zheng , Jianguo Tang , Ganpeng Li , Chunqiong Wang , Mingpeng Liu","doi":"10.1016/j.talanta.2025.128074","DOIUrl":"10.1016/j.talanta.2025.128074","url":null,"abstract":"<div><div>Two coordination polymers named α-Copper(I) Iodide-3-pyridinecarbohydrazide (α-CuI-m-iah) and β-Copper(I) Iodide-3-pyridinecarbohydrazide (β-CuI-m-iah) with varying crystal structures and tunable emission wavelengths were obtained by adjusting the ligand (3-pyridinecarbohydrazide, m-iah) -to-metal clusters (CuI) ratio during the preparation process. α-CuI-m-iah was employed for the fluorescence detection of pesticide residues flumetralin, which demonstrated high selectivity, strong resistance to interference, and a wide linear detection range (1 μM–600 μM). It also exhibited a low detection limit (0.49 μM) and a fast response time, making it effective for detecting flumetralin in water samples with recovery rates ranging from 90.53 % to 97.13 %. The detection mechanism involved competitive absorption and fluorescence resonance energy transfer (FRET) between flumetralin and α-CuI-m-iah, resulting in a decrease in fluorescence intensity. This work not only introduces two coordination polymers with tunable emission behaviors but also highlights their potential for applications in pesticide residue detection.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128074"},"PeriodicalIF":5.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-04-03DOI: 10.1016/j.talanta.2025.128052
Pengshen Jing , Qian Chen , Haifeng Ke , WenHui Xu , Yanhong Ni , Chun Mao , Runzhi Deng
{"title":"Magnesium-based micromotors for electrochemical detection of dopamine in blood","authors":"Pengshen Jing , Qian Chen , Haifeng Ke , WenHui Xu , Yanhong Ni , Chun Mao , Runzhi Deng","doi":"10.1016/j.talanta.2025.128052","DOIUrl":"10.1016/j.talanta.2025.128052","url":null,"abstract":"<div><div>In the fields of neuroscience and medicine, the accurate capture and quantitative analysis of dopamine (DA) levels in the blood are of great clinical significance for the early diagnosis of neurological dysfunctions and other pathological conditions. In this study, we developed a laccase (La) sensor based on micromotor technology to accurately detect DA content in blood. Firstly, the magnesium-based micromotor was used to endow the sensor with autonomous motion performance, which significantly increased the contact frequency between La and DA molecules in blood. Secondly, the micromotor-loaded Fe<sub>3</sub>O<sub>4</sub>/PPY/La magnetic nanoparticles provide an efficient electrical signal transmission interface for La, while the presence of polypyrrole (PPY) prevented electrode passivation caused by La. In addition, we used magnetic to collect Fe<sub>3</sub>O<sub>4</sub>/PPY/La after capture and tested it by differential pulse voltammetry. Finally, thanks to the excellent catalytic performance and high selectivity of La, the effective recognition and detection of DA in blood samples were realized, and an efficient electrochemical detection method was developed. This innovative detection scheme has a wide linear detection range and high sensitivity, which is expected to provide a more accurate diagnostic tool for health problems related to DA abnormalities, so as to better cope with and prevent related diseases.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128052"},"PeriodicalIF":5.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Voltammetric determination of lercanidipine in urine, blood plasma, and serum using hollow fiber-based liquid phase microextraction","authors":"Oleksandra Labzova , Vojtěch Hrdlička , Renáta Šelešovská , Tomáš Navrátil","doi":"10.1016/j.talanta.2025.128038","DOIUrl":"10.1016/j.talanta.2025.128038","url":null,"abstract":"<div><div>This study investigates a synergistic combination of hollow fiber-based liquid phase microextraction (HF-LPME) and voltammetric analysis using screen-printed boron-doped diamond electrodes (SP-BDDE) for the determination of antihypertensive drug lercanidipine (LCN) in urine, blood plasma, and blood serum. Optimum HF-LPME conditions were: dodecane as supported liquid membrane, Britton-Robinson buffer (BRB) pH 7 as donor solution, BRB pH 3 as acceptor solution, and extraction time 30 min. Square-wave voltammetry (SWV) of 10 μL acceptor solution at SP-BDDE was utilized with amplitude 75 mV, frequency 10 Hz, and potential step 4 mV. The calibration dependence of the combined HF-LPME-SWV method was established in the range of 1–1000 nmol L<sup>−1</sup>, with limits of quantification (<em>LOQ</em>) and detection (<em>LOD</em>) of 3.3 and 1.1 nmol L<sup>−1</sup>, respectively. The applicability of the developed method was verified by analysis of human urine, blood plasma, and blood serum samples spiked with 20 and 100 nmol L<sup>−1</sup> LCN with recoveries ranging from 93.2 to 112.9 % with respective <em>RSD</em>s 1.72–8.5 % (n = 3).</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128038"},"PeriodicalIF":5.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-04-03DOI: 10.1016/j.talanta.2025.128069
Tingting He , Daxiu Li , Yao Qin , Yi Xiang , Yike Tang , Jian Tian , Yaqin Tang
{"title":"Element-bridged dual-walker amplification for sensitive detection of multiple miRNAs by inductively coupled plasma mass spectrometry","authors":"Tingting He , Daxiu Li , Yao Qin , Yi Xiang , Yike Tang , Jian Tian , Yaqin Tang","doi":"10.1016/j.talanta.2025.128069","DOIUrl":"10.1016/j.talanta.2025.128069","url":null,"abstract":"<div><div>The simultaneous detection of multiple microRNAs (miRNAs) is critical for advancing precise disease diagnosis and treatment strategies. Herein, we introduce a novel method for the sensitive detection of multiple miRNAs using inductively coupled plasma mass spectrometry (ICP-MS), based on a platinum group element-bridged DNAzyme dual-walker amplification system. Upon binding to target miRNAs, the terminal of DNAzyme is released, triggering two DNAzyme-mediated ribonucleotide hydrolysis walking processes driven by Mg<sup>2+</sup>. These DNAzyme-walking sequences are recycled, enabling the continuous cleavage of substrate strands. After magnetic separation, the generated element pairs are quantified by mass spectrometry, enabling simultaneous and highly sensitive detection of multiple miRNAs. The method demonstrates a linear detection range from 5 fM to 10 pM with detection limits of 1.8 fM for miRNA-21, 1.1 fM for miRNA-199a, and 1.5 fM for miRNA-499, respectively.Furthermore, the approach exhibits remarkable specificity, making it suitable for detecting miRNAs in human serum. By demonstrating such a high sensitivity and specificity, this DNA walker-based sensing strategy holds significant potential for the development of versatile platforms to detect other miRNAs at varying expression levels.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128069"},"PeriodicalIF":5.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-04-02DOI: 10.1016/j.talanta.2025.128073
Muhammad Nadeem , Muhammad Faheem , Maryam Manzoor , Amna Gulzar , Muhammad Bilal , Yasir Jamil
{"title":"Machine Learning Assisted LIBS Classification of Burnt and Unburnt Paper Samples: A Forensic Perspective","authors":"Muhammad Nadeem , Muhammad Faheem , Maryam Manzoor , Amna Gulzar , Muhammad Bilal , Yasir Jamil","doi":"10.1016/j.talanta.2025.128073","DOIUrl":"10.1016/j.talanta.2025.128073","url":null,"abstract":"<div><div>A crucial part of investigating into a possible arson case is analyzing the chemical makeup of the fire's effects. In order to identify the chemical makeup of burnt materials that are frequently found in homes and businesses, the current study attempts to explore the potential of LIBS in forensic applications including burning of as well as forgery in paper-based documents. Various kinds of paper were chosen as a model sample to investigate the potential of Laser induced breakdown spectroscopy (LIBS) in forensics. First of all, LIBS spectra were recorded for unburnt samples by using Nd: YAG laser operated at 532nm, by optimizing the specific energy and delay between the laser pulse and shutter of the spectrometer, their elemental compositions were evaluated. The plasma parameters such as, plasma temperature, and electron number density were studied comprehensively to validate the local thermodynamic equilibrium condition. The three different kinds of ignition sources namely disposable lighter, candle, and gas stove were used to burn the sample to check the variation in elemental composition after the burning process for forensic applications as these sources are often present in homes and workplaces. The LIBS results suggest that all the samples have almost the same elemental composition even after using various kinds of ignition sources. Therefore, machine learning was applied to LIBS data by recording 100 datasets for each sample when ignited with different sources. PCA discriminates the samples slightly, but supervised machine learning algorithms LDA, QDA, and Linear SVM showed superior i.e., 100 % classification accuracies for various datasets that suggest the machine learning-assisted LIBS is a promising tool for forensic applications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128073"},"PeriodicalIF":5.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-04-02DOI: 10.1016/j.talanta.2025.128050
A. Cruz-Zabalegui, P. Tirado-Cantú, E.J. Alvarado-Muñoz, J.J. Alcantar-Peña, G. Martínez-Saucedo, I.R. Chávez-Urbiola
{"title":"Microfabricated Ti/Ni electrodes for non-enzymatic glucose detection: Mechanistic insights and interference analysis in blood-mimicking conditions","authors":"A. Cruz-Zabalegui, P. Tirado-Cantú, E.J. Alvarado-Muñoz, J.J. Alcantar-Peña, G. Martínez-Saucedo, I.R. Chávez-Urbiola","doi":"10.1016/j.talanta.2025.128050","DOIUrl":"10.1016/j.talanta.2025.128050","url":null,"abstract":"<div><div>Accurate glucose monitoring is essential for diabetes management, and while enzymatic sensors dominate the market, their limitations in stability and reliability under extreme conditions require alternative approaches. This study presents a non-enzymatic glucose sensor based on Ti/Ni electrodes fabricated via microfabrication techniques, designed to operate across a broad glucose concentration range (0–30 mM) and under physiological conditions. Electrochemical evaluations using cyclic voltammetry and chronoamperometry confirm the catalytic oxidation of glucose on Ni surfaces, demonstrating high sensitivity and selectivity. The sensor achieves a LoD of 1.29 mM, a LoQ of 3.93 mM, in alkaline solution. Interference analysis with common blood analytes such as uric acid, acetaminophen, and ascorbic acid, reveals that Ti/Ni electrodes outperform copper-based alternatives in minimizing cross-reactivity, meeting ISO 15197 standards for selectivity. Integrating NaOH-modified cellulose fibers for pH stabilization further supports the sensor's adaptability for in situ applications. These findings underscore the potential of Ti/Ni electrodes to enhance the development of stable, reliable, and non-enzymatic glucose sensors for clinical and wearable technologies.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128050"},"PeriodicalIF":5.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-04-02DOI: 10.1016/j.talanta.2025.128082
Mengting Liu , Zuncai Gu , Lu Qian , Kawtar Ettayri , Tao Deng , Chenhao Zhang , Jing Qian , Xingyi Huang , Chengquan Wang
{"title":"Enhanced quenching efficiency of UiO-66-NH2 over UiO-66 to engineer high-performance fluorescence aptasensor for oxytetracycline monitoring","authors":"Mengting Liu , Zuncai Gu , Lu Qian , Kawtar Ettayri , Tao Deng , Chenhao Zhang , Jing Qian , Xingyi Huang , Chengquan Wang","doi":"10.1016/j.talanta.2025.128082","DOIUrl":"10.1016/j.talanta.2025.128082","url":null,"abstract":"<div><div>Oxytetracycline (OTC), a broad class of antibacterial medication, has been widely utilized in poultry husbandry and fish farming. However, the presence of OTC residues in the food has become a matter of great concern. These residues have the potential to induce the emergence and spread of bacterial resistance, which can have far-reaching consequences for public health. In this work, we focused on the comparison of the quenching efficiency of UiO-66 and its amino group-modified counterpart UiO-66-NH<sub>2</sub>, towards the fluorescence of nitrogen-doped graphene quantum dots (NGQDs) labeled aptamer (NGQDs-apt). It was found that UiO-66-NH<sub>2</sub> demonstrated a more potent fluorescence quenching ability towards NGQDs-apt, as high as 98.8 %, compared to 69.7 % for UiO-66 without amino functionalization. This enhanced performance is attributed to the improved interaction between the amino groups on UiO-66-NH<sub>2</sub> and the NGQDs-apt, facilitating more effective electron transfer. Consequently, UiO-66-NH<sub>2</sub> was selected as the optimal quencher for the development of a highly sensitive aptasensor. This aptasensor demonstrates a quantitative detection range for OTC spanning from 0.001 to 200 μM with an exceptionally low detection limit of 0.25 nM, showing simplicity, accuracy, stability and great potential for antibiotic residue detection in food samples. This work addresses the urgent need to develop reliable methods for detecting OTC residues in food due to the potential of OTC to trigger bacterial resistance, which is crucial for food safety and public health.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128082"},"PeriodicalIF":5.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-04-02DOI: 10.1016/j.talanta.2025.128083
Peifang Chen , Yu Li , Mingwei Qin , Shuo Qi , Ning Ding , Dayun Tang , Zhouping Wang
{"title":"Inspired by enzyme-linked aptamer assay: Polydopamine coating-enhanced colorimetric-photothermal lateral flow assay for detection of enrofloxacin","authors":"Peifang Chen , Yu Li , Mingwei Qin , Shuo Qi , Ning Ding , Dayun Tang , Zhouping Wang","doi":"10.1016/j.talanta.2025.128083","DOIUrl":"10.1016/j.talanta.2025.128083","url":null,"abstract":"<div><div>Developing rapid and portable tests for enrofloxacin (ENR) residues is critical to ensuring food safety. At present, aptamer-based lateral flow analysis (Apt-LFA) has received extensive attention due to low cost and user friendliness. The study presents a novel LFA for the sensitive detection of ENR, using bovine serum albumin-enrofloxacin (BSA-ENR) conjugates as the competitor. Based on the competitive binding between ENRapt and both ENR and BSA-ENR, we developed an indirect competitive enzyme-linked aptamer assay (icELAA). Leveraging the above findings, an Apt-LFA for rapid ENR analysis was designed. Polydopamine was in situ grown on plasmonic Au nanorods to serve as signal probes (Au NRs/PDA), with improved colorimetric and photothermal responses and enhanced structural stability. The dual-readout Apt-LFA achieved a visual limit of detection of 5 ng/mL, about 20 times more sensitive than conventional Au nanoparticles-based LFA. Notably, the Au NRs/PDA-LFA simplifies the assay process of HRP-based icELAA, presenting a flexible and promising tool for extensive food safety assessment.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128083"},"PeriodicalIF":5.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-04-02DOI: 10.1016/j.talanta.2025.128060
Qiaoli Yang , Xiao Yang , Ke Guo , Guangrui Pan , Qiuzheng Du , Liang Ming , Wanhai Wang
{"title":"Live bacteria detection with high specificity by utilizing Eu3+@MIL-53 (Al) and bacteriophages-based fluorescence biosensor","authors":"Qiaoli Yang , Xiao Yang , Ke Guo , Guangrui Pan , Qiuzheng Du , Liang Ming , Wanhai Wang","doi":"10.1016/j.talanta.2025.128060","DOIUrl":"10.1016/j.talanta.2025.128060","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> (PA) infection poses a significant threat to human health and the economy, with the issue of drug resistance becoming increasingly severe. How to quickly, accurately, and inexpensively detect PA and differentiate live bacteria from dead ones is crucial for clinical follow-up treatment. To this end, we have developed a biosensor based on fluorescent metal-organic frameworks (MOFs) (Eu<sup>3+</sup>@MIL-53(Al)) and specific-phages for PA detection, where the Eu<sup>3+</sup>@MIL-53(Al) prepared by one-pot method exhibits excellent fluorescence properties. By covalently binding specific phages isolated from hospital wastewater to the EuMOF surface, we constructed a fluorescent biosensor capable of detecting PA within 15 min, with a sensitivity threshold of 2 CFU/mL. Notably, this biosensor demonstrates high specificity, accurately quantifying live PA in complex bacterial mixtures and distinguishing live from dead bacteria. Its practical application has been validated in clinical blood and stool samples. As a novel platform for clinical microbial monitoring, the Eu<sup>3+</sup>@MIL-53(Al)-phage biosensor not only holds substantial economic potential but also carries significant public health implications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128060"},"PeriodicalIF":5.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}