Talanta最新文献

{"title":"Electrochemical genomagnetic assay for detection of SARS-CoV-2 RNA using a disposable microfluidic platform","authors":"Daniel Júnior Almeida dos Santos ,&nbsp;Tássia Regina de Oliveira ,&nbsp;Henrique Pott-Junior ,&nbsp;Matias Eliseo Melendez ,&nbsp;Ester Cerdeira Sabino ,&nbsp;Ronaldo Censi Faria","doi":"10.1016/j.talanta.2025.128186","DOIUrl":"10.1016/j.talanta.2025.128186","url":null,"abstract":"<div><h3>Background</h3><div>The COVID-19 pandemic exposed the world to one of the greatest challenges in our history, causing profound social and economic impacts. With the advance of the pandemic, it became evident that there was a need for new diagnostic tests for monitoring and controlling the disease. In this sense, we describe the development of a genomagnetic assay based on the use of a fully disposable electrochemical microfluidic device for detection of RNA from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in saliva samples.</div></div><div><h3>Results</h3><div>A simple and low-cost disposable microfluidic platform (DμP) containing unmodified carbon-based electrodes was developed and coupled to a 3D holder containing neodymium magnets for detection of SARS-CoV-2 RNA in saliva samples. The device allows up to 8 simultaneous detections using the genomemagnetic assay, providing sensitivity with a limit of detection of 0.44 fmol L⁻¹ and a limit of quantification of 1.44 fmol L⁻¹, with a linear range of 5.0 fmol L⁻¹ to 200.0 nmol L⁻¹. The proposed assay has been successfully applied for the diagnosis of COVID-19 in cohorts of positive and negative individuals, showing excellent agreement with the results obtained by reverse transcription-polymerase chain reaction (RT-PCR). The genomagnectic assay showed the ability to discriminate between healthy individuals and patients infected with SARS-CoV-2, showing 82.4 % and 100.0 % of clinical sensitivity and specificity, respectively.</div></div><div><h3>Significance</h3><div>The method developed allows a simple, low-cost, and quantitative detection of viral RNA requiring no transcription or DNA amplification steps showing excellent reproducibility. The genomagnetic assay proposed can be an alternative tool for the diagnosis and monitoring of COVID-19 and even could be easily adapted for detection of other single-stranded RNA viruses.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128186"},"PeriodicalIF":5.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid and specific fluorescent probe based on ESIPT-AIE-active for copper ion quantitative detection in food and environmental samples","authors":"Xiaomei Ren ,&nbsp;Ji Liu ,&nbsp;Yating Zhang ,&nbsp;Jianing Zhang ,&nbsp;Yuanlan Yang ,&nbsp;Wenjian Yang ,&nbsp;Jianhui Liu ,&nbsp;Anxiang Su ,&nbsp;Hui Xu ,&nbsp;Zhenwei Yuan","doi":"10.1016/j.talanta.2025.128188","DOIUrl":"10.1016/j.talanta.2025.128188","url":null,"abstract":"<div><div>In the field of food safety, the identification and measurement of active components in food is a pressing issue. The concentration of copper ions (Cu²⁺) in the environment is closely linked to food safety, and overall biological health. Therefore, developing rapid and accurate analytical techniques to monitor Cu²⁺ in food is of great significance. In this study, two fluorescent probes L-2 and L-3 were synthesized through a simple Schiff base condensation reaction. And L-3 demonstrated better anti-interference ability to Cu²⁺ than that of L-2. Meanwhile, spectroscopic experiments showed that L-3 possessed an extremely low detection limit (LOD) and low limits of quantification (LOQ) (LOD = 92.79 nM, LOQ = 309.33 nM), and quickly respond time (&lt;30 s). Probe L-3 for monitoring effectively quantitatively identified Cu²⁺ in food and environmental samples, achieving an accuracy rate ranging from 84.42% to 117.45% and precision with a relative standard deviation (RSD) of less than 6.0%. The accuracy had been validated using the inductively coupled plasma-mass spectrometry (ICP-MS). Simultaneously, a WeChat Mini Program has been developed to detect total copper content in food samples based on fluorescence values, enabling consumers to evaluate food safety more intuitively. Moreover, L-3 also facilitated the quantitative visualization of Cu²⁺ in biological systems, underscoring its compatibility and practicality.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128188"},"PeriodicalIF":5.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an indirect competitive enzyme immunoassay for detection of a plant elicitor methiadinil","authors":"Yue Zhang ,&nbsp;Rongyan Shen ,&nbsp;Yuting Huang ,&nbsp;Zhihong Wang ,&nbsp;Liangfu Tang ,&nbsp;Zhijin Fan","doi":"10.1016/j.talanta.2025.128149","DOIUrl":"10.1016/j.talanta.2025.128149","url":null,"abstract":"<div><div>Plant elicitors with distinctive benefits including low toxicity, small application dosages and an innovative mode of action are considered as green agrochemicals by stimulating the host plant immunity. Several new plant elicitors have been developed for tobacco protection against virus attack. Among them, as a milestone in Chinese plant elicitor development, methiadinil (IUPAC: 4-methyl-<em>N</em>-(5-methylthiazol-2-yl)-1,2,3-thiadiazole-5-carboxamide) shows broad application prospects. Because of its low application dosage, its residue determination requires diverse highly sensitive method. Here we developed an indirect enzyme-linked immunosorbent assay (ELISA) method for methiadinil residue determination with the lowest detection limit of 10.69 ng/mL and a linear detection range between 21.84 ng/mL of IC₂₀ and 1513.81 ng/mL of IC₈₀ by a rational hapten designation. Meanwhile, this indirect ELISA method showed good specificity with low cross-reactivity for methiadinil analogue determination and a good correlation with the HPLC method for tobacco sample measurement. This sensitive, rapid and accurate methiadinil residue determination method in tobacco leaves is of great significance for the monitoring of methiadinil in tobacco and guidance for other environmental sample determination and the rational application of methiadinil.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128149"},"PeriodicalIF":5.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable arginine sensing on polyvinyl alcohol membrane based on aggregation-induced emission enhancement of carbon dots","authors":"Dou Yang ,&nbsp;Tong Shao ,&nbsp;Peng Li ,&nbsp;Xiaoshuang Wang ,&nbsp;Suyuan Zeng ,&nbsp;Min Hong ,&nbsp;Rui Li ,&nbsp;Qiaoli Yue","doi":"10.1016/j.talanta.2025.128177","DOIUrl":"10.1016/j.talanta.2025.128177","url":null,"abstract":"<div><div>Carbon dots (CDs) were highly promised nanomaterials for application in food analysis, owing to their exceptional optical properties, superior photostability, and negligible biological toxicity. N-doped CDs (NCDs) were synthesized via a straightforward hydrothermal method utilizing folic acid as only one precursor material. NCDs exhibited deep blue fluorescence under an ultraviolet lamp with a quantum yield (QY) of 14.54 %. In the presence of arginine (Arg), there was a noticeable redshift of 10 nm in the maximum emission wavelength of NCDs, accompanied by fluorescence transition from dark blue to bright blue. The QY of NCDs was 16.71 % in the presence of Arg. In other words, NCDs showed aggregation-induced emission enhancement (AIEE) in the presence of Arg. The fluorescence enhancement exhibited a strong linear correlation with Arg concentration within the range of 0–300 μM, and the limit of detection was 0.31 μM. Furthermore, to facilitate Arg detection, a portable Arg sensor was developed using a polyvinyl alcohol membrane-assisted design. The sensor demonstrated satisfactory performance in detecting Arg in real samples. These findings highlighted the versatile capabilities of NCDs with AIEE properties, paving the way for their potential applications in food analysis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128177"},"PeriodicalIF":5.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in capillary electrophoresis for plant analysis","authors":"Petr Tůma","doi":"10.1016/j.talanta.2025.128171","DOIUrl":"10.1016/j.talanta.2025.128171","url":null,"abstract":"<div><div>Capillary and microchip electrophoresis plays an important role in the analysis of the chemical composition of plants and nutrient soils, which finds applications in plant physiology, agrochemistry, medicine, toxicology and food science. Electrophoretic methods are used to determine minerals such as nutrients, heavy metal ions, primary and secondary metabolites, herbicides, phytohormones, peptides, proteins and extracellular vesicles. Progress is particularly evident in the following topics: i) development of mobile electrophoretic analysers for field-based monitoring of soil mineral supply, ii) direct analysis of xylem sap without sample treatment, iii) coupling of capillary and microchip electrophoresis with mass spectrometry for comprehensive metabolome and proteome characterization, iv) determination of secondary metabolites as biologically active compounds with a range of therapeutic and toxicological effects, v) monitoring of herbicides and their degradation dynamics, vi) research on plant exudates, extracellular vesicles and specific protein interactions.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128171"},"PeriodicalIF":5.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual enzyme-mimicking bimetallic MOF for selective SERS detection of L-DOPA in human serum based on cascade catalytic reaction","authors":"Shuojiang Liu,&nbsp;Ying Chen,&nbsp;Xingliang Cheng,&nbsp;Gongke Li,&nbsp;Yuling Hu","doi":"10.1016/j.talanta.2025.128178","DOIUrl":"10.1016/j.talanta.2025.128178","url":null,"abstract":"<div><div>Surface-enhanced Raman scattering (SERS) in conjunction with nanozymes has emerged as a powerful technique for bioanalysis due to their signal conversion and amplification effect. However, clinical applications continue to encounter challenges such as inadequate selectivity. Herein, we proposed a novel approach for the selective SERS detection of L-DOPA in human serum with dual enzyme-mimicking MOF-919(Fe–Cu). This material exhibits both catechol oxidase-like and peroxidase-like activities, enabling it to catalyze catecholic compounds to <em>o</em>-quinones and subsequently oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) molecules into SERS-active ox-TMB. Ultrasonic-assisted synthesis ensures uniform and dense loading of AgNPs within MOF-919(Fe–Cu), thereby enhancing SERS activity. A sensitive and selective method for SERS detection of L-DOPA was developed. The catechol oxidase-like activity of MOF-919(Fe–Cu) provides ideal selectivity and anti-interference to L-DOPA, while the cascade catalytic system transforms weak SERS signals of L-DOPA into amplified SERS signals of ox-TMB. The mechanism of dual enzyme-mimicking activity was further discussed. The detection process was optimized to achieve high sensitivity towards L-DOPA with a linear range 0.5–100 μmol/L and low limit of detection (LOD) of 0.16 μmol/L. Furthermore, it has been applied in serum sample of Parkinson's patients, demonstrating excellent recovery and low relative error compared to HPLC-UV. This approach offers an economical, efficient and accurate strategy for the detection of L-DOPA and provides valuable insights for the integration of selective multifunctional nanozyme and sensitive SERS detection method.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128178"},"PeriodicalIF":5.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in quantum dot-based fluorescence sensors for environmental and biomedical detection","authors":"Samaneh Ghanbarlou ,&nbsp;Davood Kahforoushan ,&nbsp;Hossein Abdollahi ,&nbsp;Payam Zarrintaj ,&nbsp;Adam Alomar ,&nbsp;Carlos Villanueva ,&nbsp;Seyed Mohammad Davachi","doi":"10.1016/j.talanta.2025.128176","DOIUrl":"10.1016/j.talanta.2025.128176","url":null,"abstract":"<div><div>This review explores the evolution and application of fluorescence sensors based on quantum dots (QDs) for detecting environmental and biological analytes across diverse real-world scenarios and complex sample matrices and also categorizes different types of quantum dots, such as carbon dots (C-dots), graphene quantum dots (GQDs), and metal-doped QDs and examines their properties, including tunable fluorescence, low toxicity, and photostability, which make them ideal for a variety of applications. Key sensing mechanisms, including Förster Resonance Energy Transfer (FRET) and fluorescence quenching, are discussed alongside innovations like paper-based, ratiometric, and turn-on/turn-off sensors. Additionally, case studies are provided to showcase the application of these sensors in environmental and biomedical fields, where they provide rapid, sensitive, and cost-effective solutions. This review presents the potential of quantum dot-based fluorescence sensors to transform analytical detection technologies, offering new opportunities in environmental monitoring, bioimaging, and public health safety.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128176"},"PeriodicalIF":5.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Matter","authors":"","doi":"10.1016/S0039-9140(25)00624-1","DOIUrl":"10.1016/S0039-9140(25)00624-1","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"Article 128134"},"PeriodicalIF":5.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionic liquid-assisted synthesis of In2O3 nanoparticles for ultra-fast detection of unsymmetrical dimethylhydrazine","authors":"Weiyi Bu ,&nbsp;Yan Zhang ,&nbsp;Dan Huang ,&nbsp;You Zhou ,&nbsp;Na Liu ,&nbsp;Wenjiang Han ,&nbsp;Xiaohong Chuai ,&nbsp;Zhijie Zhou ,&nbsp;Changhua Hu ,&nbsp;Geyu Lu","doi":"10.1016/j.talanta.2025.128140","DOIUrl":"10.1016/j.talanta.2025.128140","url":null,"abstract":"<div><div>Due to the unique physiochemical properties of ionic liquids, they are extensively used as agent or solvent in the synthesis process of nanomaterials. The tailored growth process of functional nanomaterials might result in performance modification for them. In this work, In₂O₃ nanoparticles were prepared via the hydrothermal method and subsequent calcination (400 °C, 500 °C, 600 °C and 700 °C). An ionic liquid (1-hexadecyl-3-methylimidazolium chloride, [C₁₆Mim] Cl) was introduced in the hydrothermal process. After calcination, trace of [C₁₆Mim] Cl inclusion in In₂O₃ was pyrolyzed with Cl residue (Cl–In₂O₃). In compare with control sample synthesized without [C₁₆Mim] Cl (pure In₂O₃), In₂O₃-600 (Cl–In₂O₃ calcined at 600 °C) exhibited an excellent UDMH sensing performance at 225 °C. The fabricated sensor achieved a high response value (71.0 ± 2.1 to 100 ppm), rapid response time (2 s), good selectivity and a low theoretical limit of detection (1.72 ppb) to UDMH gas. Various characterizations, in regard to oxygen vacancy, surface texture structure, energy band structure and surface acidity, were utilized to analyze the cause for enhanced performance. The ionic liquid inclusion increases the contents of adsorbed oxygen species and improves the adsorption capacity to UDMH gas. This work indicates that the synthesized In₂O₃ nanoparticles could be the potential candidates for rapid UDMH detection in some specific application scenarios.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128140"},"PeriodicalIF":5.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly selective detection of trace Lead(II) ions based on MoS2 QDs/TiO2 NRs photoelectrochemical sensing","authors":"Jiawei Chen ,&nbsp;Jun Wang ,&nbsp;Ganghai Ni ,&nbsp;Jin Liu ,&nbsp;Min Liu ,&nbsp;Liangxing Jiang","doi":"10.1016/j.talanta.2025.128167","DOIUrl":"10.1016/j.talanta.2025.128167","url":null,"abstract":"<div><div>MoS₂ quantum dots/TiO₂ nanorods (MQDs/TNRs) is designed as a photoelectrochemical (PEC) platform for trace Pb²⁺ detection. High photocurrent density is achieved by means of Pb²⁺ induced formation of the band-tail states near the conduction band minimum of MoS₂ and relieved cliff-like band alignment of the MQDs/TNRs p-n junctions. Among the cations of Pb²⁺, Li⁺, Na⁺, K⁺, Co²⁺, Cu²⁺, Zn²⁺, Ni²⁺, Cd²⁺, Fe³⁺, Mn³⁺, Pb²⁺ shows the highest selectivity due to the lowest adsorption energy of Pb²⁺ about −1.119 eV at the edge S sites of MoS₂. Between the relative increase in the photocurrent density and the logarithm of Pb²⁺ concentration there are outstanding linear relationships in the concentration range of 1 μM–1000 μM, with a low detection limit of 48 nM. In addition, high recoveries of Pb²⁺ are realized at the concentrations of 20 μm for 97.95 % and 200 μm for 96.81 % in the sample analysis of tap water. This sensor offers a fast and stable response for trace lead ions detection in environmental governance and water treatment.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128167"},"PeriodicalIF":5.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}