Talanta最新文献

{"title":"Immunosensors for dual tumor biomarker detection based on ternary electrochemiluminescence using confined CeO2 nanozyme as Co-reactant enhancers for luminol-O2 system","authors":"Chao Lu ,&nbsp;Xue Fan ,&nbsp;Fengna Xi ,&nbsp;Yucheng Zhou","doi":"10.1016/j.talanta.2026.129524","DOIUrl":"10.1016/j.talanta.2026.129524","url":null,"abstract":"<div><div>Sensitive and parallel individual detection of multiple tumor biomarkers is critical for early diagnosis and therapeutic monitoring of pancreatic cancer. Herein, a ternary electrochemiluminescence (ECL) system was developed by employing nanochannel-confined cerium dioxide (CeO₂) nanozyme as co-reactant enhancers for the luminol-dissolved oxygen (DO) system, enabling parallel individual immunoassays of carcinoembryonic antigen (CEA) and carbohydrate antigen 199 (CA199). Vertically-ordered mesoporous silica film (VMSF) was rapidly grown on an indium tin oxide (ITO) electrode, forming nanochannel array with thickness of 97 nm and diameters of 2.7 nm. CeO₂ nanozyme was synthesized <em>in situ</em> within the VMSF nanochannels <em>via</em> electrochemical deposition. Transmission electron microscopy (TEM), high-angle annular dark-field scanning TEM (HAADF-STEM), and energy-dispersive X-ray spectroscopy (EDX) elemental mapping confirmed CeO₂ nanozyme was confined in nanochannels with high dispersion. CeO₂ nanozyme exhibited peroxidase-like catalytic activity and enhanced the ECL intensity of the luminol-DO system by 13.9-fold under near-neutral conditions through catalysis of both oxygen reduction and luminol oxidation. After derivatization of the VMSF outer surface with epoxy groups, antibodies specific to CEA or CA199 were covalently immobilized to construct two immunorecognition interfaces. Target binding formed antigen-antibody complexes, increasing interfacial resistance and hindering diffusion of the ECL emitters, enabling a signal-off immunoassay mode. The resulting immunosensors displayed wide detection linear ranges from 0.1 pg mL⁻¹ to 100 ng mL⁻¹ for CEA and 0.1 × 10⁻³ mU mL⁻¹ to 1.0 × 10⁴ mU mL⁻¹ for CA199, with limit of detection (LOD) of 1.3 fg mL⁻¹ and 0.5 × 10⁻⁴ mU mL⁻¹, respectively. Both immunosensors exhibited good selectivity, reproducibility, and storage stability.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129524"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hollow Sn-doped ZnO nanotubes for synergistically enhanced sensing performance toward 3-hydroxy-2-butanone with preliminary exploration of a multi-parameter intelligent system","authors":"Xiao-Xue Lian,&nbsp;Jing-Wen Liu,&nbsp;Yan Li,&nbsp;Shu-Mei Yang","doi":"10.1016/j.talanta.2026.129512","DOIUrl":"10.1016/j.talanta.2026.129512","url":null,"abstract":"<div><div>Targeted detection of the 3-hydroxy-2-butanone (3H-2B) biomarker represents a feasible and reliable strategy for monitoring <em>Listeria monocytogenes</em>, a highly virulent foodborne pathogen that poses severe threats to public health and food safety. The significance of this study lies in addressing the urgent demand for rapid, sensitive, and on-site detection of <em>Listeria monocytogenes</em>, as traditional detection methods are often time-consuming, labor-intensive, and unsuitable for field applications. Herein, we successfully prepared Sn-doped ZnO hollow nanotubes via an electrospinning process, and further designed a multi-parameter intelligent sensing system based on this material for portable 3H-2B detection. A key improvement of our work is the synergistic integration of Sn doping and morphological engineering, which dramatically enhances gas-sensing performance: Sn doping introduces a high density of oxygen vacancies and free charge carriers, while the tailored hollow nanotube configuration maximizes the accessibility of active sites and facilitates rapid gas diffusion across the sensing interface. The optimized 3% Sn-doped ZnO sensor exhibits excellent 3H-2B sensing performance, with an optimal response of 155.1 to 100 ppm 3H-2B at 200 °C, a rapid response time of 1 s, and a low detection limit of 100 ppb, significantly outperforming undoped ZnO and other Sn-doped counterparts. This sensing system shows promising application prospects in food safety supervision, catering industry, and food processing workshops, enabling rapid on-site monitoring of <em>Listeria monocytogenes</em> contamination. This study not only establishes Sn-doped ZnO hollow nanotubes as a superior sensing platform for 3H-2B but also opens a new avenue for the rapid, sensitive detection of foodborne pathogens, providing technical support for food safety guarantee.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129512"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemometrics-assisted validation of a high-throughput method for quantitative analysis of multiclass phytohormones across plant matrices by UHPLC-QQQ-MS/MS for targeted metabolomics","authors":"Tingting Tong ,&nbsp;Lili He ,&nbsp;Liya Qiao ,&nbsp;Yaqin Zhou ,&nbsp;Jieqiong Li ,&nbsp;Jie Liang ,&nbsp;Jine Fu ,&nbsp;Shugen Wei ,&nbsp;Gaowu Huang ,&nbsp;Xianghua Xia","doi":"10.1016/j.talanta.2026.129541","DOIUrl":"10.1016/j.talanta.2026.129541","url":null,"abstract":"<div><div>The quantification of phytohormones in plant samples is significantly challenging because of their trace concentrations and the complex plant matrix. This study establishes and optimizes a simple and fast method for simultaneous quantification of multiclass phytohormones using ultra high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-QQQ-MS) for high-throughput targeted metabolomics analysis. It is characterized by simplicity and operational feasibility, and it involves sequential steps of ultrasound-assisted extraction, centrifugation, and nitrogen flow-drying. The assessment of sample preprocessing method employs the technique for order preference by similarity to an ideal solution (TOPSIS) chemometrics-assisted approach, and methanol containing 1% formic acid was selected as the optimal extraction solvent. Quantitative analysis is performed via a multiple reaction monitoring method. The method was validated satisfactory for 31 phytohormones, the specific concentration levels used to estimate recovery and precision were 0.25–200 ng/mL, and the results showed good linearity (correlation coefficients: 0.9910–0.9999), sensitivity (LOD: 0.01–2.87 ng/g; LOQ: 0.02–8.70 ng/g), accuracy (recovery rates: 64.50–129.67%), precision (relative standard deviations &lt;20%, except for IAA-Trp), and its applicability to a variety species of medicinal plant was verified, including <em>Taxillus chinensis</em> (DC.) Danser, <em>Ophiopogon japonicus</em> (L.f.) Ker-Gawl, <em>Citrus grandis</em> “Tomentosa,” <em>Plumeria rubra L.</em> “Acutifolia,” and <em>Pogostemon cablin</em> (Blanco) Benth. In the real samples analyzed, auxins concentrations exceeded 4.07 μg/g, salicylic acids concentrations ranged from 0.027 to 79.7 μg/g, and concentrations of other categorize of phytohormones were below 1 μg/g. The established method demonstrated high throughput, accuracy, stability, and reliability. It can be extended to cover additional phytohormone types and diverse application scenarios. This method serves as a potent tool for investigating the function of multiclass phytohormones in the regulation of secondary metabolite accumulation in medicinal plants, elucidating complex signal transduction mechanisms and interaction networks, and laying the groundwork for improving the quality of Chinese medicinal materials.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129541"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic bead-assisted extraction combined with ID-LC-MS/MS for simultaneous quantification of fat-soluble vitamins A, D, E, and K in human serum","authors":"Qingqing Pan ,&nbsp;Jing Lin ,&nbsp;Chunlan Tang ,&nbsp;Tao Yu ,&nbsp;Min Shen ,&nbsp;Guangliang Wu ,&nbsp;Huoyan Ji","doi":"10.1016/j.talanta.2026.129520","DOIUrl":"10.1016/j.talanta.2026.129520","url":null,"abstract":"<div><div>This study developed and validated a high-throughput method based on automated magnetic solid-phase extraction (MSPE) with isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) for the simultaneous and accurate quantification of five fat-soluble vitamins (VA, 25(OH)D₃, 25(OH)D₂, VE, and VK₁) in human serum. For the MSPE, a functionalized silica-based magnetic core-shell material was synthesized, featuring a poly (polystyrene-co-divinylbenzene-co-N-vinylpyrrolidone) coating and a covalently immobilized 2-vinylbenzofuran affinity ligand linked via a hydrophilic polyethylene glycol spacer. This design facilitated efficient enrichment and purification of the target analytes. The validated method demonstrated excellent specificity, with complete chromatographic separation, without significant matrix effects or interferences. It exhibited superior sensitivity and linearity, with a satisfactory linear response (<em>R</em>² &gt; 0.995 for all analytes) and a low limit of quantification (0.15 ng/mL-0.49 μg/mL). Recoveries were between 90.48 % and 101.95 %, while precision was satisfactory with intra-day and inter-day coefficients of variation (CV) below 7.14 % and 4.68 %, respectively. The accuracy of the method was further demonstrated by analyzing NIST standard reference material SRM 968f and SRM 972a, with a mean bias within ±7 %. Compared to traditional liquid-liquid extraction (LLE) process, the automated MSPE process efficiently extracted the target analytes from serum within 11 min through systematic optimization. Furthermore, method comparison showed a good agreement between the clinical routine LLE method and the MSPE method established. In addition, the method performed well in external quality assessment schemes, also confirming high accuracy and reliability. This robust methodology provides a powerful analytical tool for the precise monitoring of fat-soluble vitamins and nutritional status assessment in clinical practice and has the potential to promote the development of laboratory automation.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129520"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential adoption of electrochemical biosensors for cancer DNA biomarker detection in liquid biopsies: A systematic review","authors":"Anouk Peymen ,&nbsp;Thijs Van der Snickt ,&nbsp;Alejandro Valverde ,&nbsp;Scott Ailliet ,&nbsp;Karen Zwaenepoel ,&nbsp;Pieter Mestdagh ,&nbsp;Karolien De Wael","doi":"10.1016/j.talanta.2026.129488","DOIUrl":"10.1016/j.talanta.2026.129488","url":null,"abstract":"<div><h3>Background</h3><div>The detection of actionable DNA biomarkers in liquid biopsies is essential for advancing precision oncology. While conventional techniques such as PCR and NGS are highly accurate, their high cost, complexity, and slow turnaround time limit widespread clinical applications. Electrochemical biosensors present a promising alternative through their portability, affordability, and rapid analysis capabilities.</div></div><div><h3>Objective</h3><div>This systematic review evaluates the current status and potential clinical adoption of electrochemical biosensors for detecting cancer DNA biomarkers in liquid biopsies, focusing on their sensitivity and specificity compared to standard PCR-based methods.</div></div><div><h3>Methods</h3><div>A comprehensive search across PubMed, Scopus, and Web of Science was conducted up to August 2025, yielding 1723 articles. After applying PRISMA guidelines with strict inclusion and exclusion criteria, 31 studies were selected. The quality of the selected studies was assessed using the SURE checklist. Data on biosensor design, amplification strategies, detection limits, and clinical applicability were extracted.</div></div><div><h3>Results</h3><div>Most biosensors achieved femtomolar to attomolar detection limits (LODs), reaching the required sensitivity limits for germline and somatic mutations respectively These LODs were achieved primarily via signal amplification strategies using redox mediators, nanomaterials, and strand displacement assays. Target amplification was less common and did not demonstrate consistent superiority. Specificity was enhanced through modified probes, enzymatic cleavage, magnetic separation, and blocking agents, though assessment methods varied. In general, covalently immobilized probes improved stability and sensitivity. However, most assays were validated in buffer with synthetic DNA, limiting clinical relevance.</div></div><div><h3>Conclusions</h3><div>With respect to their performance, electrochemical biosensors exhibit considerable potential for clinical integration. In particular when integrating different signal amplification strategies, such as a synergistic approach of redox mediators and nanocomposites, or combining target and signal amplification to reach the required sensitivity limits. Nonetheless, their clinical integration requires evaluation in complex biological samples, more thorough selectivity studies, and rigorous validation on patient samples. When standard validation assays can be provided and performed, electrochemical biosensing assays hold great promise for the detection of cancer DNA biomarkers in liquid biopsies.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129488"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sensitive pH fluorescent probe with large Stokes shift and narrow transition for lysosome imaging during cell cycle","authors":"Bochao Chen ,&nbsp;Jixiang Zhang ,&nbsp;Yifan Zhong ,&nbsp;Xi Liu ,&nbsp;Ji Xu ,&nbsp;Ningning Cui ,&nbsp;Jin Zhou","doi":"10.1016/j.talanta.2026.129534","DOIUrl":"10.1016/j.talanta.2026.129534","url":null,"abstract":"<div><div>Intracellular pH plays a crucial role in regulating cellular functions, and its subtle changes can influence normal physiological activities and contribute to various diseases. Although numerous probes have been developed for detecting microenvironmental pH in living cells, their broad dynamic ranges induce blurred imaging boundaries and may generate false-positive results, compromising the accurate detection of subtle pH fluctuations. In response to these issues, we designed and synthesized a novel 1,8-naphthalic anhydride-based pH fluorescent probe (<strong>NAM</strong>) that demonstrated highly sensitive responses to pH changes with a narrow transition range spanning just 2.4 pH units. The pH responsiveness of <strong>NAM</strong> originated from the deprotonation of its aniline group, which induced an enhanced intramolecular charge transfer (ICT) process. This mechanism enables <strong>NAM</strong> to exhibit a remarkable 15-fold fluorescence enhancement at 569 nm with a pKa value of 6.71 and a significant Stokes shift of 112 nm. Furthermore, <strong>NAM</strong> specifically targets lysosomes and was successfully employed to detect intracellular pH fluctuations induced by lipopolysaccharide (LPS), nutrient deprivation and chloroquine treatment. Significantly, we demonstrate for the first time that <strong>NAM</strong> achieved pH imaging analysis across different cell cycle phases. During the transition from G0/G1 to S phase, intracellular pH gradually alkalinizes, followed by rapid acidification in the G2/M phase. These observations reflected the dynamic balance between energy storage and consumption during cell cycle progression. These distinctive features position <strong>NAM</strong> as a promising tool for investigating physiological and pathological processes associated with abnormal pH variations.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129534"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HRP-induced wide wavelength-tunable in situ fluorogenic system and its immunoassay for cTnI in clinical samples","authors":"Jingjing Yin ,&nbsp;Yuehua Wang ,&nbsp;Shanshan Zhu ,&nbsp;Xiaoxue Liu,&nbsp;Yujie Sun,&nbsp;Hong Yang,&nbsp;Yifei Ma,&nbsp;Jinhua Liu","doi":"10.1016/j.talanta.2026.129511","DOIUrl":"10.1016/j.talanta.2026.129511","url":null,"abstract":"<div><div>The development of wavelength-tunable fluorescence immunoassays for sensitive biomarker detection remains a significant challenge in clinical diagnostics. In this work, we present an HRP-induced <em>in situ</em> fluorogenic immunoassay platform based on the rapid reaction between <em>p</em>-phenylenediamine (PPD) analogues and 6-hydroxyquinoline (6-Hy). The proposed mechanism involves HRP-catalyzed generation of hydroxyl radicals from H₂O₂, which subsequently oxidize PPD to form fluorescent copolymer nanoparticles (FCNPs) with 6-Hy. By strategically modifying the PPD structure with various substituents, we achieved discrete and tunable emission of FCNPs, with maxima spanning from 380 to 600 nm (Δλ ≈ 220 nm). Using cardiac troponin I (cTnI) as a model antigen and zinc-coordination nanoparticles (ZnNPs) as carriers, the immunoassay demonstrated exceptional performance, with a wide dynamic range (0.5-125 ng/mL) and an ultralow detection limit (0.17 ng/mL) for cTnI. The platform was successfully validated in human serum samples, showing excellent correlation with clinical standards. This work not only establishes a novel strategy for wavelength-programmable immunoassays but also provides a versatile platform for advancing biomarker detection in clinical diagnostics.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129511"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended-gate FET biosensor utilizing ionic strength engineering for sensitive prostate-specific antigen detection","authors":"Sheng-Chun Hung ,&nbsp;Kai-Chun Hung ,&nbsp;Chia Kai Lin","doi":"10.1016/j.talanta.2026.129531","DOIUrl":"10.1016/j.talanta.2026.129531","url":null,"abstract":"<div><div>This work presents an extended-gate field-effect transistor (EGFET) biosensor functionalized with prostate-specific antigen (PSA) aptamers. It employs ionic strength engineering to enhance electrostatic coupling and improve detection sensitivity. By systematically adjusting the concentration of phosphate-buffered saline (PBS), we modified the electric double-layer (EDL) structure and Debye length to investigate their effects on charge screening and signal transduction. The proposed biosensor achieved a detection limit of 0.408 ng/mL in 0.001 × PBS, which is significantly lower than the clinical threshold of 4 ng/mL. Additionally, it exhibited an impressive nearly fivefold increase in sensitivity as the ionic strength decreased from 1 × to 0.001 × PBS. These enhancements can be attributed to extended Debye screening and reduced electrostatic shielding in low-ionic environments, facilitating more efficient field-effect coupling between the aptamer–PSA complex and the transistor channel. Temporal noise characterization, conducted through Allan deviation analysis, revealed an optimal averaging range of 30 to 80 s. This range effectively balances noise suppression and signal fidelity. In summary, this study demonstrates a straightforward and effective strategy for regulating ionic strength to enhance the transduction efficiency of BioFET-based biosensors, offering a practical route toward high-performance and label-free PSA biosensing in biomedical diagnostics.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129531"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver nanoflower SERS substrate incorporating polyoxometalate as an internal standard for selective quantification of cationic dyes","authors":"Jie Wang,&nbsp;Chun-Hui Zhang,&nbsp;Yun-Dong Cao,&nbsp;Jun-Peng Wang,&nbsp;Jia-Yuan Zhang,&nbsp;Cai-Li Lv,&nbsp;Hong Liu,&nbsp;Guang-Gang Gao","doi":"10.1016/j.talanta.2026.129529","DOIUrl":"10.1016/j.talanta.2026.129529","url":null,"abstract":"<div><div>Silver nanoparticles (AgNPs) serve as traditional surface-enhanced Raman scattering (SERS) substrates, exhibiting advantages such as low cost, facile synthesis, and high sensitivity. However, conventional AgNPs-based SERS substrates encounter significant limitations regarding quantitative analysis and attaining adequate selectivity in practical applications. In this work, a novel silver nanoflower SERS substrate (<strong>W₁₂@AgNF</strong>s) with high stability and uniform morphology was fabricated by the chemical reduction of a structure-defined silver clusters precursor. The Raman signals originating from polyoxometalates (POMs) within the <strong>W₁₂@AgNFs</strong> substrate serve as a reliable internal standard for quantitative detection. Furthermore, due to the substantial negative charge of POMs, the SERS substrate exhibits selective adsorption towards cationic dyes. The ratiometric SERS signal intensities are proportional to the logarithm of the cationic dye concentrations, facilitating analyte-specific quantitative SERS analysis. Under optimized conditions, this substrate allows for the selective and quantitative detection of crystal violet dye at a concentration as low as 10⁻¹¹ M, with its detection performance notably outperforming most existing testing methods. This study proposes an innovative approach for designing POM-Ag nanocomposites as highly efficient SERS substrates with integrated internal standards, enabling the progression of SERS spectroscopy toward the challenging quantitative and selective analyses of cationic hazards.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129529"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-activity OXD-like MnOx nanozymes: H2O2-free signal amplification strategy for ultrasensitive colorimetric assay of antioxidants in fruit juice","authors":"Wenteng Qiao ,&nbsp;Caihong Yin ,&nbsp;Boyuan Chen ,&nbsp;Yue Zhai ,&nbsp;Xiuling Song","doi":"10.1016/j.talanta.2026.129514","DOIUrl":"10.1016/j.talanta.2026.129514","url":null,"abstract":"<div><div>Total antioxidant capacity represents a critical parameter for assessing food quality and freshness, while conventional detection methodologies are often constrained by operational intricacy or insufficient efficiency. Herein, we developed a rapid and user-friendly colorimetric sensing platform based on manganese oxide (MnOx) nanozymes for the quantification of antioxidants in fruit juice samples. MnOx nanozymes can efficiently catalyze the oxidation of the colorless substrate 3,3′,5,5′-tetramethylbenzidine, yielding a blue reaction product; however, the presence of antioxidants significantly suppresses this catalytic activity, inducing concentration-dependent color shifts in the reaction system. After systematic optimization of key reaction parameters (e.g., nanozyme concentration, TMB dosage, reaction temperature, and incubation time), the proposed platform achieved a detection limit of 1.79 μM, with a naked - eye visual detection limit of 5 μM and a total detection time of &lt;10 min. Practical validation in real fruit juice samples verified the accuracy of the developed method for antioxidant quantification, as evidenced by recovery rates of 88.76%–109.10% and RSDs of all below 3.44%.This work introduces a novel, efficient, and stable approach for the rapid monitoring of TAC in food products, exhibiting considerable potential for food quality control and safety assessment.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"304 ","pages":"Article 129514"},"PeriodicalIF":6.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}