TalantaPub Date : 2025-01-01Epub Date: 2024-09-20DOI: 10.1016/j.talanta.2024.126913
Han Lu, Zhenbo Xiang, Hailong Pang, Qiang Ren
{"title":"Rational design of Ag-doped MnFe<sub>2</sub>O<sub>4</sub>/HNTs for peroxidase-mimicking activity and colorimetric sensing of uric acid in human serum.","authors":"Han Lu, Zhenbo Xiang, Hailong Pang, Qiang Ren","doi":"10.1016/j.talanta.2024.126913","DOIUrl":"10.1016/j.talanta.2024.126913","url":null,"abstract":"<p><p>Mimicking enzyme have significantly advanced sensing assays by replicating native enzyme functions, yet achieving both high catalytic activity and easy recyclability remains a challenge. In this study, Ag-doped MnFe<sub>2</sub>O<sub>4</sub>/halloysite nanotubes (HNTs) were rationally designed as a novel nanozyme by depositing in-situ Ag and MnFe<sub>2</sub>O<sub>4</sub> nanoparticles onto HNTs. The resulting nanocomposite exhibited excellent peroxidase-like activity along with magnetic properties. Leveraging these features, a highly efficient and sensitive colorimetric system for detecting uric acid (UA) was developed. The Ag-doped MnFe<sub>2</sub>O<sub>4</sub>/HNTs catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H<sub>2</sub>O<sub>2</sub>, causing a color change from colorless to blue. The system showed a linear absorbance response to UA concentrations ranging from 1 to 20 μM, with a detection limit of 59 nM. Mechanistic studies revealed that reactive oxygen species intermediates (<sup>1</sup>O<sub>2</sub>) were generated through the decomposition of H<sub>2</sub>O<sub>2</sub>, leading to peroxidase-like activity in the Ag-doped MnFe<sub>2</sub>O<sub>4</sub>/HNTs. The assay was successfully applied to detect UA in human serum with recoveries over 99.68 %. This study indicates the successful application of Ag-doped MnFe<sub>2</sub>O<sub>4</sub>/HNTs for colorimetric UA detection in human serum. This research introduces a novel approach for designing recyclable, high-performance mimicking enzyme and establishes an effective colorimetric sensing platform for UA detection in human serum.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126913"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-01-01Epub Date: 2024-09-24DOI: 10.1016/j.talanta.2024.126937
Yue Kong, Xinyue Qian, Xiao Mei, Jun Ma, Kang Wu, Anping Deng, Jianguo Li
{"title":"Electrochemiluminescence immunoassay system based on PCN-224-Mn and gold-platinum bimetallic nanoflowers for sensitive detection of ochratoxin A.","authors":"Yue Kong, Xinyue Qian, Xiao Mei, Jun Ma, Kang Wu, Anping Deng, Jianguo Li","doi":"10.1016/j.talanta.2024.126937","DOIUrl":"10.1016/j.talanta.2024.126937","url":null,"abstract":"<p><p>In this work, a novel Electrochemiluminescence Immunosensor was constructed using PCN-224-Mn and gold-platinum nanoflowers (AuPt NFs) for the ultrasensitive detection of ochratoxin A (OTA). PCN-224 modified with Mn (II) was synthesized as a probe material. The interaction efficiency of PCN-224 with S<sub>2</sub>O<sub>8</sub><sup>2-</sup> was also greatly improved. AuPt NFs were used as the substrate material for the electrodes. It has favorable biocompatibility, large specific surface area and can bind more antigen. Also greatly increased the electroactive surface area and conductivity of the electrode. OTA was detected using a competitive immunoassay strategy, in which OTA in the sample competes with the encapsulated antigen for a finite number of antibodies. ECLIA for the detection of OTA was designed to be highly sensitive, with a linear range from 0.0002 ng mL<sup>-1</sup> to 1000 ng mL<sup>-1</sup> and a LOD as low as 0.067 pg mL<sup>-1</sup>. In addition, it was evident from the electrochemical analyses that PCN-224-Mn had a stronger and more stable ECL signal compared to the plain PCN-224. The successful preparation of specific, sensitive and reproducible ECL immunosensors confirms the great promise for the detection of OTA or other small molecule mycotoxins.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126937"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-01-01Epub Date: 2024-09-24DOI: 10.1016/j.talanta.2024.126943
Rong Zhao, Qiang Niu, Ghulam Murtaza, Guling Zhang, Yuping Yang
{"title":"Integrated identification and detection of hydration state and its evolution using terahertz technology.","authors":"Rong Zhao, Qiang Niu, Ghulam Murtaza, Guling Zhang, Yuping Yang","doi":"10.1016/j.talanta.2024.126943","DOIUrl":"10.1016/j.talanta.2024.126943","url":null,"abstract":"<p><p>The accurate detection of dehydration processes in hydrated drugs can reveal various intermolecular vibration modes mediated by hydrogen bonds between water molecules and other components, which underpin the further development of pharmaceutical science, food safety and biophysics. Herein, terahertz (THz) technology is utilized to investigate the dehydration state of d(+)-Raffinose pentahydrate (Rf·5H<sub>2</sub>O), in conjunction with imaging-based point by point scanning data acquisition and barcodes methods, to establish an innovative platform integrated identification, trace detection, and application capabilities. Our study demonstrates that the dehydration process of Rf·5H<sub>2</sub>O can be dynamically monitored through the evolution of its THz absorption peaks, offering more precise results compared to XRD and Raman spectroscopies. Moreover, the absorbance spectra data collected at each individual pixel is utilized to build visualized THz images, achieving an ultralow minimum content required for detection of 0.032 μg/(50 μm)<sup>2</sup>. Additionally, we introduce a THz spectra-barcode conversion system that not only ensures efficient electronic recordkeeping but also enhances user readability, thereby facilitating the practical applications of THz technology.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126943"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-01-01Epub Date: 2024-10-09DOI: 10.1016/j.talanta.2024.127006
Tingting Han, Sini Chen, Songlin Yang, Tao Song, Xuliang Lin, Qin Yu, Dongxue Han, Johan Bobacka, Li Niu
{"title":"Determination of silver ions in distilled water by stripping voltammetry at a Nafion-coated gold electrode in combination with quartz crystal microbalance and electrochemical impedance spectroscopy.","authors":"Tingting Han, Sini Chen, Songlin Yang, Tao Song, Xuliang Lin, Qin Yu, Dongxue Han, Johan Bobacka, Li Niu","doi":"10.1016/j.talanta.2024.127006","DOIUrl":"10.1016/j.talanta.2024.127006","url":null,"abstract":"<p><p>Here, we propose a stripping voltammetric method for Ag<sup>+</sup> ion determination in distilled water utilizing screen-printed Au electrodes coated with Nafion (Nafion/Au screen-printed electrodes). The concentration of Ag<sup>+</sup> in pure water was determined by linear sweep voltammetry (LSV) after silver deposition on the Nafion/Au electrode. The anodic stripping peak current increased linearly with the concentration of Ag<sup>+</sup> ion in the range from 1 ppm to 22 ppm. The LSV oxidative peak current was increased by extending the silver deposition time from 300 s to 500 s. Repetitive LSV measurements revealed satisfactory reproducibility of the Nafion/Au screen-printed electrodes. The detection mechanism was elucidated by recording mass changes of the Nafion/Au electrode with a quartz crystal microbalance (QCM), and by determining changes in the low-frequency capacitance of the Nafion/Au electrode by electrochemical impedance spectroscopy (EIS). The observed changes in mass and capacitance confirmed Ag<sup>+</sup> accumulation and release processes at the Nafion/Au electrodes, in good agreement with stripping voltammetry. The combination of stripping voltammetry, QCM and EIS allowed a detailed characterization of the ion transfer, deposition and stripping processes at the Nafion/Au electrodes in presence of Ag<sup>+</sup> ions. The Nafion/Au screen-printed electrode enabled voltammetric determination of low Ag<sup>+</sup> concentrations in distilled water, without any sample pretreatment nor addition of supporting electrolyte.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127006"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-01-01Epub Date: 2024-10-17DOI: 10.1016/j.talanta.2024.127062
Haifang Mao, Yucheng Zhou, Zhengyang Xu, Yun Zhao
{"title":"Enhanced enantioselective separation of racemic menthol via reverse-phase high-performance liquid chromatography: Method development and computational insights for pre-screening.","authors":"Haifang Mao, Yucheng Zhou, Zhengyang Xu, Yun Zhao","doi":"10.1016/j.talanta.2024.127062","DOIUrl":"10.1016/j.talanta.2024.127062","url":null,"abstract":"<p><p>A robust and efficient enantioselective separation of racemic menthol was achieved on a standard C<sub>18</sub> column with reverse-phase high-performance liquid chromatography (RP-HPLC) and UV detector. (R)-α-hydroxy-4-methylbenzeneacetic acid was utilized as the pre-column derivatization reagent. The impact of mobile phase composition on diastereomer selectivity was thoroughly investigated, resulting in a high resolution of 2.11 under optimized conditions. The method was rigorously validated for linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). Notably, a separation pre-screening mechanism (SPM) and a prediction model was developed based on density functional theory (DFT) studies. This model elucidated the relationship between molecular polarity differences (△MPI) and chromatographic behavior, facilitating the interpretation and prediction of racemic menthol resolution with various chiral derivatization reagents. The present work not only presents an efficient and economical approach for menthol enantiomeric separation, but also offers valuable insights for the innovative design and advancement of chromatographic methodologies for a wide array of chiral enantiomers.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127062"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-01-01Epub Date: 2024-10-16DOI: 10.1016/j.talanta.2024.127051
Wenmin Zhang, Zhiyong Wang, Lan Zhang
{"title":"Interface-assisted synthesized covalent organic framework film for efficient extraction of microcystins in aquatic organisms.","authors":"Wenmin Zhang, Zhiyong Wang, Lan Zhang","doi":"10.1016/j.talanta.2024.127051","DOIUrl":"10.1016/j.talanta.2024.127051","url":null,"abstract":"<p><p>Covalent organic framework (COF) film-based solid-phase extraction (F-SPE) has garnered great attention in sample pretreatment. However, harsh synthesis conditions of COF films have severely hindered their potential applications. In this study, a kind of COF (TPB-DMTP) films were fabricated via a liquid-liquid interfacial synthesis method at a mild condition. The obtained films exhibited excellent extraction performance towards microcystins (MCs, an algal toxin) due to their porous structure, high specific surface area and abundant accessible adsorption sites. Coupled TPB-DMTP films-based F-SPE with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), a sensitive and environment-friendly analytical method was established for MCs detection. Under the optimal conditions, this method possessed wide linear ranges (2.0-800.0 pg mL<sup>-1</sup>) with good linearity (R ≥ 0.9991), low limits of detection (0.8-3.0 pg mL<sup>-1</sup>) and satisfactory precision (RSDs ≤7.1 %), which then successfully applied for MCs detection in actual aquatic organism samples. Trace amounts of MC-RR (42.4 pg mL<sup>-1</sup>) and MC-YR (14.6 pg mL<sup>-1</sup>) were detected in the mussels. The results demonstrate the excellent application potential of COF films in sample pretreatment.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127051"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enhanced electrochemiluminescence of CdS QDs encapsulated in IRMOF-3 for sensitive detection of human epithelial protein 4.","authors":"Xinyu Liu, Dongyu Han, Feng Jiang, Shanghua Liu, Yueyuan Li, Zhen Xu, Qing Liu, Yueyun Li, Qin Wei","doi":"10.1016/j.talanta.2024.127052","DOIUrl":"10.1016/j.talanta.2024.127052","url":null,"abstract":"<p><p>The advancement of pragmatic and highly-sensitive electrochemiluminescence (ECL) biosensors depends upon signal tags with high and stable signal intensity. Herein, enhanced ECL emission was obtained by encapsulating the dual-stabilizer-capped CdS QDs in a metal-organic framework (MOF), which served as a valid ECL signal tag for detecting biomarkers. Dual-stabilizer-capped CdS QDs reduce dangling bonds on the surface and improved the ECL emission. Furthermore, functionalized isoreticular metal-organic framework-3 (IRMOF-3) can not only load a large quantity of CdS QDs through the encapsulation capability but also serves as a co-reaction accelerator to promote the formation of more SO<sub>4</sub><sup>•-</sup> from the S<sub>2</sub>O<sub>8</sub><sup>2-</sup>, further improving the ECL emission of QDs, while the integrated design of IRMOF-3 co-reaction accelerator and CdS QDs effectively shortens the electron transfer pathway and reduces the energy consumption in ECL system. Using human epithelial protein 4 (HE4) as the model of analysis, the biosensor demonstrated a broad linear range (50 fg mL<sup>-1</sup>∼50 ng mL<sup>-1</sup>) and a low detection limit (9.89 fg mL<sup>-1</sup>) under optimal operating conditions. The study provides an effective and alternative method to improve the ECL efficiency of QDs, significantly broadening their potential applications in sensing analysis, medical diagnostics, and bioimaging.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127052"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mechanobiology studies of bladder tumor cells using laterally squeezing microfluidic flow cytometry.","authors":"Zhiwen Zheng, Shuaihua Zhang, Yiming Liu, Ziyu Han, Hang Qi, Xuexin Duan, Zhihong Zhang","doi":"10.1016/j.talanta.2024.127090","DOIUrl":"10.1016/j.talanta.2024.127090","url":null,"abstract":"<p><p>The deformability and uptake capability of cells are critical indicators of their biomechanical properties and functional behaviors, particularly in tumor heterogeneity and cancer research. Here, we introduce a microfluidic flow cytometry platform integrated with a laterally adjustable squeezing structure for the characterization of bladder tumor cells (including 5637 and EJ cell lines) and uroepithelial cells (SV-HUC-1 cell line). The deformability of these cell types under varying channel width conditions was clearly assessed using this platform. The results demonstrated that tumor cells exhibited higher deformability compared to uroepithelial cells, with the EJ cell line exhibiting the greatest difference. Furthermore, the relationship between the malignancy, deformability, and uptake capability of bladder cells was explored through co-cultivation experiments with 2 μm particles. As the malignancy increased, the cells became more deformable and exhibited stronger phagocytic capability with particles. Subsequently, the heterogeneity of tumor cells was investigated by analyzing the deformability of phagocytic and non-phagocytic subpopulations within EJ cells. The developed microfluidic platform offers a promising high-throughput method to assess the biomechanical and phagocytic characteristics of cells, providing valuable insights into tumor cell biology, and potentially improving clinical status of urinary cytology examinations for bladder cancer.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127090"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-01-01Epub Date: 2024-09-18DOI: 10.1016/j.talanta.2024.126906
Xishun Peng, Qixin Chen, Xinghua Li, Li Yang, Yiheng Yuan, Cheng Zuo, Zhangyu Zhou, Zhongchen Bai
{"title":"A semiconductor SERS sensor of corrosion-resistant PPy/GO composite film by electrochemical growth for detecting crystal violet residues in fresh fish tissue.","authors":"Xishun Peng, Qixin Chen, Xinghua Li, Li Yang, Yiheng Yuan, Cheng Zuo, Zhangyu Zhou, Zhongchen Bai","doi":"10.1016/j.talanta.2024.126906","DOIUrl":"10.1016/j.talanta.2024.126906","url":null,"abstract":"<p><p>Crystal violet (CV) residues in Marine food have produced a severe health threat in human life. In this study, we proposed a semiconductor surface-enhanced Raman scattering (SERS) sensor of corrosion-resistant Polyaniline/Graphene oxide (PPy/GO) film by electrochemical growth method to detect CV residues in fresh fish tissue. A PPy/GO dispersion solution was one-step deposited on a stainless steel sheet surface by electrochemical polymerization process to form a PPy/GO composite film acting as a semiconductor SERS substrate. Since the substrate of PPy/GO film was mainly composed of GO sheet without other metals, it had a good corrosion resistance. The SERS enhancement factor and charge transfer intensity P<sub>CT</sub> of PPy/Go SERS substrate for CV molecules were up to 1.18 × 10<sup>6</sup> and 0.903, respectively. Furthermore, the limit of detection (LOD) of PPy/GO SERS substrate could reach 1.58 nM. In addition, SERS sensor of PPy/GO film could identify CV residues in fresh fish tissues, and its recovery rate was 91.8 %-107 %. This preparing method and detecting method we proposed PPy/GO SERS substrate provide a new pathway for detecting CV residues in Marine food.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126906"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TalantaPub Date : 2025-01-01Epub Date: 2024-09-21DOI: 10.1016/j.talanta.2024.126919
Dieudonne Tanue Nde, Dhananjaya Merum, Gyawali Ghanashyam, Jean Pierre Ndabakuranye, Mohamed A Habila, Arghya Narayan Banerjee, Sang Woo Joo, Theophile Niyitanga, Haekyoung Kim
{"title":"Electrochemical sensor featuring PdFeCo<sub>1-x</sub>ONPs on carbon paper for the sensitive determination of indole-3-lactic acid levels in serum samples.","authors":"Dieudonne Tanue Nde, Dhananjaya Merum, Gyawali Ghanashyam, Jean Pierre Ndabakuranye, Mohamed A Habila, Arghya Narayan Banerjee, Sang Woo Joo, Theophile Niyitanga, Haekyoung Kim","doi":"10.1016/j.talanta.2024.126919","DOIUrl":"10.1016/j.talanta.2024.126919","url":null,"abstract":"<p><p>A highly sensitive and selective electrochemical sensing platform with self-assembled porous 3-D trimetallic (Pd, Fe, and Co) hybrid anchored on a cost-effective and high-conducting carbon paper (CP) synthesized via a facile and cost-effective hydrothermal impregnation and thermal reduction technique was developed for determining indole-3-lactic acid (ILA) levels in buffer and serum samples. Before the analytical phase, the composite (PdFeCo<sub>1-x</sub>ONPs@CP electrode) was thoroughly characterized, and different methods were used to investigate the electrochemical properties. The combination of tri-metallics with CP-fibers improved sensing capacities in the linear range of 0.05-30 μM, with sensitivity and limits of detection of and 0.165 ± 0.013 μA/μM and 7.8 ± 0. 2 nM, respectively, towards ILA determination. Furthermore, the developed sensing platform was utilized for the analyses of ILA in sigma, human normal, and alcohol use disorder patients' serum samples. Liquid chromatography in tandem with mass spectrometry was equally used to quantify ILA levels in the serum samples and the results of both methods were compared.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126919"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}