Talanta最新文献

{"title":"Terahertz Let-7a specific sensing and DNA differentiation enabled by Q-BIC metasurface and phenylboronic acid-gold nanoprobe","authors":"Weinan Shi ,&nbsp;Fei Fan ,&nbsp;Jia Zhang ,&nbsp;Lu Zhang ,&nbsp;Guohui Xiao ,&nbsp;Zhong Ren ,&nbsp;Juanli Zhao ,&nbsp;Xianghui Wang","doi":"10.1016/j.talanta.2025.128577","DOIUrl":"10.1016/j.talanta.2025.128577","url":null,"abstract":"<div><div>Nucleic acids are genetic molecules that carry biological information, the development of accurate nucleic acid detection technology is of great significance for the treatment of some diseases. Here, we proposed a novel scheme to specific detect Let-7a (miRNA) and simultaneous discrimination from DNA based on terahertz (THz) metasurface. The metasurface is designed for supporting quasi-bound states in continuum (Q-BIC) resonance, which can effectively improve the sensing performance. Numerical simulation consistent with experimental results confirms the Q-BIC is induced by the symmetry broken between the metal wires. To specific detect trace amount Let-7a, the PBA-AuNPs probe developed by modifying Au nanoparticles (AuNPs) with phenylboronic acid (PBA), is integrated into metasurface for signal amplification. Our results show that highly sensitive and selective detection of Let-7a is achieved, the detection limit can reach to 0.01 nM. More importantly, due to the selective binding of the nanoprobe, the sensitivity for Let-7a is more than 3 times that of DNA sharing the same sequence. Even in serum samples, the frequency shift of resonance still maintains a good linear relationship with the logarithm of the Let-7a concentration. This miRNA-targeted PBA-AuNPs amplification technology offers a versatile platform with translational potential across disciplines, particularly advancing emerging THz metasurface-based biosensing.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128577"},"PeriodicalIF":5.6,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced peroxidase-like activity of single-atom dispersed cobalt in carbon nitride for glutathione detection via chemiluminescence signals of non-luminol system","authors":"Zhiqiang Wang ,&nbsp;Suxing Jiao ,&nbsp;Yingzi Liu,&nbsp;Xulin Liu,&nbsp;Jing Wu","doi":"10.1016/j.talanta.2025.128580","DOIUrl":"10.1016/j.talanta.2025.128580","url":null,"abstract":"<div><div>Chemiluminescence (CL) analysis is regarded as a significant analytical tool due to its advantages, including no background interference, high sensitivity, easy operation, and cost-effectiveness. However, low emission intensity hinders its wide application. Therefore, it is necessary to develop sensitizers to enhance CL performance. In this work, one kind of single-atom catalysts (SACs) was prepared by atomically dispersing Co atoms on carbon nitride (Co–C₃N₄ SACs) and applied in enhancing the CL intensity of NaHSO₃–H₂O₂ system for 107 times. The mechanism for CL enhancement of Co–C₃N₄ SACs was studied by systematic experiments and theoretic calculation. Co–C₃N₄ SACs exhibited strong peroxidase-like activity in H₂O₂ decomposition, generating reactive oxygen species (ROS) as the key step for CL signal enhancement. The constructed Co–C₃N₄ SACs-NaHSO₃-H₂O₂ CL system was utilized for the detection of glutathione (GSH), demonstrating a linear range of 10 μM–500 μM and a good linear relationship (<em>R</em>² = 0.9978). This approach was successfully applied in detecting GSH in human serum, demonstrating excellent recoveries and repeatability. Our work has extended the application of SACs in non-luminol CL system and explored their capability in developing CL sensors.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128580"},"PeriodicalIF":5.6,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid isotopic analysis of uranium microparticles via SP-ICP-TOF-MS","authors":"Jordan S. Stanberry ,&nbsp;Sarah E. Szakas ,&nbsp;Hunter B. Andrews ,&nbsp;Lyndsey Hendriks ,&nbsp;Brian W. Ticknor ,&nbsp;Rachel A. Bergin ,&nbsp;Shawna K. Tazik ,&nbsp;Philip Kegler ,&nbsp;Stefan Neumeier ,&nbsp;N. Alex Zirakparvar ,&nbsp;Daniel R. Dunlap ,&nbsp;Benjamin T. Manard","doi":"10.1016/j.talanta.2025.128578","DOIUrl":"10.1016/j.talanta.2025.128578","url":null,"abstract":"<div><div>Inductively coupled plasma – time-of-flight – mass spectrometry (ICP-TOF-MS) was employed for the isotopic analysis of uranium particles of varying ²³⁵U enrichment levels. Here, a single particle (SP)-based introduction scheme was employed such that individual particles, in a suspension, were analyzed. The uranium oxide microparticles were comprised of depleted uranium (DU, ²³⁵U/²³⁸U of 0.0017316(14)), natural uranium (NU, ²³⁵U/²³⁸U of 0.0072614(39)), and low enriched uranium (LEU, ²³⁵U/²³⁸U of 0.051025(15)). The percent relative difference of the SP-ICP-TOF-MS measured isotopic ratios compared to the expected values for the DU, NU, and LEU particle populations were 8.75, 0.12, and 1.23 %, respectively. After characterization, the DU and NU particles were doped within a complex sample matrix (Arizona Test Dust) containing Fe, Ti, Al, and Si particles, among others. Then, the suspension was analyzed via SP-ICP-TOF-MS and the detected particles were classified as DU or NU based on their measured ²³⁵U/²³⁸U ratio. In the same analysis, the matrix particles (i.e., Al, Fe, and Ti) were detected, demonstrating the simultaneous nuclide detection provided by the measurement platform. The presented SP-ICP-TOF-MS methodology for uranium particle characterization proved to be a high throughput method for detecting and isotopically discerning uranium particles with varying enrichment levels, in a complex matrix.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128578"},"PeriodicalIF":5.6,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual micron-sized coal aerosol particle for quantitative analysis based on hollow laser trapping-LIBS and machine learning","authors":"Jiaqiang Du ,&nbsp;Tianlong Zhang ,&nbsp;Hua Li ,&nbsp;Min Wang ,&nbsp;Tianguo Wang ,&nbsp;Fengguang Li ,&nbsp;Tingting Chen","doi":"10.1016/j.talanta.2025.128565","DOIUrl":"10.1016/j.talanta.2025.128565","url":null,"abstract":"<div><div>Fine particulate matter is one of the major pollutants emitted from coal combustion. Particulate matter and gaseous pollutants emitted from coal combustion are one of the main pollutants that are concerned. Laser-induced breakdown spectroscopy (LIBS) is a powerful and versatile analytical tool for real-time, simultaneous, and all-element detection. However, its broader applications are constrained by spectral interference, matrix effects, weak spectral intensity and so on. To address this challenge, multi-element quantitative analysis methods based on hollow laser trapping assisted LIBS signal enhancement of fiber collimated system were established by machine learning. The influence of five different spectral preprocessing methods and three different variable selection methods on the prediction performance of the RF calibration model was investigated. The Savitzky-Golay convolution derivative-variable importance projection-random forest (SG-VIP-RF) (Fe) and first-order derivative-variable importance measurement-random forest (D1st-VIM-RF) (Ca) calibration models were constructed based on the optimal spectral preprocessing method and variable selection method. The prediction performance of Fe and Ca elements are shown as follows: Fe (R_p² = 0.9861, MREP = 0.0477, RMSEP = 1.408 %) and Ca (R_p² = 0.9580, MREP = 0.0627, RMSEP = 2.1461 %), and their relative standard deviation (RSD) values are 2.2 % and 5.2 %, respectively. The results demonstrate that the RF calibration model based on the optical fiber collimated LIBS signal enhancement method is successfully applied to the quantitative analysis of standard coal samples. It is expected to provide theoretical basis and technical support for in-situ online rapid monitoring of coal-fired energy materials, and further promote the wide application of LIBS in on-line monitoring fields such as environmental monitoring, geological exploration and metallurgical analysis, adhere to green and low-carbon sustainable development, and help promote the goal of carbon peak carbon neutralization.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128565"},"PeriodicalIF":5.6,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and application of flexible wearable sensors based on optical fibers","authors":"Zefeng Hou ,&nbsp;Jianzhang Liu ,&nbsp;Yifei Liao ,&nbsp;Jingjing Gong ,&nbsp;Chengli Li ,&nbsp;Miaomiao Li ,&nbsp;Huan Liu ,&nbsp;Qing Huang","doi":"10.1016/j.talanta.2025.128576","DOIUrl":"10.1016/j.talanta.2025.128576","url":null,"abstract":"<div><div>Optical fiber flexible wearable sensors are extensively utilized in healthcare, sports training, smart textiles, and environmental monitoring, owing to their lightweight, portability, biocompatibility, and excellent stretchability. These sensors are distinguished by their high sensitivity, accuracy, and non-invasive monitoring capabilities, excelling at detecting subtle mechanical changes like respiratory rate and muscle activity. Additionally, they offer significant advantages regarding biocompatibility and resistance to electromagnetic interference. Recent research has concentrated on enhancing the performance and flexibility of optical fiber sensors by incorporating novel materials such as polydimethylsiloxane, polymethyl methacrylate, and hydrogels, which improve their stretchability and optical transparency. Furthermore, integrating machine learning and Internet of Things (IoT) technologies has enabled optimized data processing, facilitating real-time, precise monitoring of physiological parameters. However, challenges related to sensor integration, stability, and cost persist. Addressing these issues is crucial for establishing optical fiber wearable sensors as reliable clinical tools. This paper reviews the latest advancements in optical fiber flexible wearable sensors, discussing their working principles, manufacturing processes, and applications in healthcare, sports, and smart textiles. It also highlights challenges related to sensor design, energy management, data transmission, and integration with artificial intelligence, offering recommendations to improve sensor performance and broaden their application scope.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128576"},"PeriodicalIF":5.6,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Programmable flow heart cutting solid phase extraction using polydopamine coated microfibrous sorbent as a front-end to UHPLC for direct determination of xenobiotic residues in surface water","authors":"Lucie Chocholoušová Havlíková ,&nbsp;Petr Chocholouš ,&nbsp;Jakub Erben ,&nbsp;Pavel Holec ,&nbsp;František Švec ,&nbsp;Dalibor Šatínský","doi":"10.1016/j.talanta.2025.128571","DOIUrl":"10.1016/j.talanta.2025.128571","url":null,"abstract":"<div><div>Automation and hyphenation of multiple steps were used for the sensitive determination of xenobiotic residues in surface waters. The online hyphenated sequential injection analysis instrumentation used surface-modified microfibrous sorbent for the effective solid phase extraction of 1.0 mL of sample into 100 μL zone, then subjected to the reversed-phase ultra-high performance liquid chromatography (SIA-SPE-UHPLC). The SPE column was packed with polypropylene microfibers and coated with polydopamine by rinsing with the dopamine solution. The freshly prepared column was used for highly reproducible extraction of more than 200 samples in the SIA-SPE-UHPLC method. The matrix calibration covered the expected range with limits of detection in the range 0.1–0.9 μg/L. The method precision was &lt;13 % RSD (at 3 concentration levels), and the method accuracy was in the range 86.5–114.8 %. The sample throughput of the method was 5 samples/hour when the separation took 12 min, including a 5 min SPE step of the next sample run in parallel. The performance of the developed method was demonstrated with the analysis of fifteen river water samples spiked with eleven residues of xenobiotics (ivermectin, fenbendazole, praziquantel, ibuprofen, diclofenac, 17α-ethynylestradiol, androstenedione, carbamazepine, omeprazole, sotalol, and caffeine) at the levels typical for such monitoring. Initial sample treatment prior to the automated analysis consisted only of methanol addition, pH adjustment, and centrifugation. The innovations in the manifold and process that contribute to the modern and effective analysis of environmental samples are discussed.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128571"},"PeriodicalIF":5.6,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the effects of endogenous non-starch polysaccharide on the temperature-cycled retrogradation behavior of yam starch using asymmetrical flow field-flow fractionation coupled with multidetector","authors":"Mu Wang ,&nbsp;Liu Yang ,&nbsp;Tinghui Yin ,&nbsp;Siyu Wang ,&nbsp;Yueqiu Li ,&nbsp;Tongtong Liu ,&nbsp;Haiyang Dou","doi":"10.1016/j.talanta.2025.128568","DOIUrl":"10.1016/j.talanta.2025.128568","url":null,"abstract":"<div><div>Starch retrogradation is a complex process, affected by several factors including storage temperature and the presence of non-starch components (such as polysaccharide). In this study, the effects of the endogenous yam non-starch polysaccharide (YNSP) with different concentrations on the yam starch (YS) retrogradation behavior were investigated by asymmetrical flow field-flow fractionation (AF4), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Furthermore, the effects of isothermal and temperature-cycled storage on the retrogradation of YNSP + YS blend were systematically studied. An effect mechanism of the endogenous YNSP on the YS retrogradation during temperature-cycled storage was proposed. The results revealed that 4/-20 °C temperature-cycled storage facilitated YS retrogradation, owing to the formation of starch-rich regions during frozen storage. The mechanical squeezing force caused by the size growth of ice crystal during long-term frozen storage encouraged starch molecules to constantly rearrange, promoting YS retrogradation. Moreover, the YNSP can not only interact with amylose and amylopectin molecules to form aggregates that could promote YS short-term retrogradation, but also compete with amylopectin for water molecules, which might enhance the syneresis process during repeated freeze-chill storage, thereby facilitating YS retrogradation. The results suggested that a combination of AF4, FTIR, and XRD is a powerful method not only for monitoring the interaction between YNSP and YS, but also for investigating the effects of YNSP content and temperature on YS retrogradation. The present study might offer a theoretical foundation and guidance for the application of YNSP to starchy products.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128568"},"PeriodicalIF":5.6,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement in detection stability and prediction accuracy of laser-induced breakdown spectroscopy based on plasma acoustic correction using a quartz tuning fork","authors":"Peichao Zheng ,&nbsp;Zhicheng Sun ,&nbsp;Jinmei Wang ,&nbsp;Guanghui Chen ,&nbsp;Zhi Yang ,&nbsp;Biao Li","doi":"10.1016/j.talanta.2025.128572","DOIUrl":"10.1016/j.talanta.2025.128572","url":null,"abstract":"<div><div>To improve the stability and accuracy of quantitative analysis in laser-induced breakdown spectroscopy (LIBS), a correction method of laser induced breakdown spectroscopy using a quartz tuning fork was developed. In this experiment, Cr, Cu, Ni and Mn in steel samples were selected as the target analytes. The laser-induced plasma acoustic and spectral signals were simultaneously acquired using a quartz tuning fork and spectrometer, with the acoustic signal subsequently was used to correct the spectral signal. The results indicated that, compared to the original spectral intensities, the average relative standard deviation (ARSD) of the four elements decreased from 7.07 % to 5.02 %. The average coefficient of determination (R²) of the calibration curves improved by 1.02 %, rising from 0.976 to 0.986. The average relative error (ARE) of the predicted values decreased from 23.16 % to 11.46 %. After acoustic correction using the quartz tuning fork, the R² and the average prediction error of the calibration curve are both superior to those of the internal standard method. The results demonstrated that acoustic correction based on the quartz tuning fork can significantly enhance the stability of spectral signals and effectively improve the accuracy of calibration models.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128572"},"PeriodicalIF":5.6,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of four classes of amphiphilic surfactants by solid phase extraction and spectrophotometric detection at nanomolar levels: environmental applications","authors":"Jim Grisillon ,&nbsp;Laurie Michel ,&nbsp;Julien de Barry ,&nbsp;Barbara Nozière ,&nbsp;Julien Dron ,&nbsp;Anne Monod ,&nbsp;Fabien Robert-Peillard","doi":"10.1016/j.talanta.2025.128575","DOIUrl":"10.1016/j.talanta.2025.128575","url":null,"abstract":"<div><div>Surfactants are natural and anthropogenic compounds commonly found in all environmental compartments and can influence cloud formation due to their surface-active properties. In this work, a new method for the sensitive and selective quantification of 4 different classes of amphiphilic surfactants was developed, based on a new solid-phase extraction (SPE) procedure with a graphitized carbon black sorbent and optimized spectrophotometric methods using commercial ion-pair reagents and liquid-liquid extraction. The sequential elution used in the SPE step enabled separate quantification of cationic, non-ionic, weak anionic and strong anionic surfactants. The spectrophotometric methods of detection of all classes of surfactants were optimized. A new method was developed for strong anionic surfactants using Toluidine blue O. Significant improvements were also made to existing methods for weak anionic and non-ionic surfactants using methylene blue and iron thiocyanate, respectively. Limits of detection of 0.08, 0.076, 0.91 and 0.20 nmol were achieved for cationic, non-ionic, weak anionic and strong anionic surfactants, respectively. A classification according to the acidity of the anionic group was proposed to distinguish synthetic surfactants (strong acids) from biosurfactants (weak acids). Issues related to interfering species, losses during filtration steps were also addressed, and a new filtration method with polyethylene frits was demonstrated to improve surfactants recoveries for aerosol analysis, with recoveries above 80 % for all types of surfactants. The procedure was applied to real environmental samples, including seawater and freshwater samples, aerosols extracts, and cloud water. Surfactants were successfully detected in all samples, with total concentrations between 12.1 nM and 495 nM for aqueous samples and between 48.4 pmol m⁻³ and 443 pmol m⁻³ for aerosol samples. Anionic surfactants were found to be the major constituents in all environmental matrices, but low concentrations of cationic and non-ionic surfactants were also detected in several samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128575"},"PeriodicalIF":5.6,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Nb2CTx MXene nanomaterial-supported MIP sensor for detection of HIF–PHI inhibitor roxadustat in biological and pharmaceutical samples","authors":"Mahdi Gharibi ,&nbsp;Ahmet Cetinkaya ,&nbsp;Havva Nur Gurbuz ,&nbsp;Aytekin Uzunoglu ,&nbsp;Esen Bellur Atici ,&nbsp;Sibel A. Ozkan","doi":"10.1016/j.talanta.2025.128573","DOIUrl":"10.1016/j.talanta.2025.128573","url":null,"abstract":"<div><div>Roxadustat (ROX), a hypoxia-inducible factor prolyl hydroxylase inhibitor (HIF–PHI), effectively treats anemia in chronic kidney disease (CKD) by increasing hemoglobin levels by activating the body's natural hypoxic response. This work details the development and manufacturing of an electrochemical sensor based on molecularly imprinted polymers (MIP) and supported by MXene for the detection of ROX. Additionally, MIP-based sensors were created to identify ROX in standard solutions and biological samples. In the MIP-based electrochemical sensor developed using the photopolymerization (PP) method on the glassy carbon electrode (GCE) surface were utilized 2-dimensional Nb₂CT_x MXene (2D Nb₂CT_x) as a pore former, 3-amino phenylboronic acid (3-APBA) served as the functional monomer, and ROX as the template molecule. Under optimized experimental conditions, the designed sensor exhibited a linearity range of 1.0 × 10⁻¹³ – 1.0 × 10⁻¹² M. Interference studies confirmed the superior selectivity of the MIP-based sensor for ROX, demonstrating its effectiveness in various binary mixtures. The recovery values of the MIP-based sensors were found to be 100.80% for commercial tablet samples and 99.53% for commercial serum samples. Moreover, the relative selectivity coefficient (k′) of the proposed sensor was calculated. This recently designed sensor provides a promising method for quickly, sensitively, economically, and selectively analyzing ROX. The results obtained in high selectivity and recovery studies make the developed sensor a valuable tool for various applications in clinical and pharmaceutical environments. In addition, this study achieved better sensitivity than other studies in the literature and is the first electrochemical sensor prepared using Nb₂CT_x MXene nanomaterials-supported MIP technology for ROX measurement.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128573"},"PeriodicalIF":5.6,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}