Insights into the effects of endogenous non-starch polysaccharide on the temperature-cycled retrogradation behavior of yam starch using asymmetrical flow field-flow fractionation coupled with multidetector

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-07-10 DOI:10.1016/j.talanta.2025.128568

Mu Wang , Liu Yang , Tinghui Yin , Siyu Wang , Yueqiu Li , Tongtong Liu , Haiyang Dou

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Abstract

Starch retrogradation is a complex process, affected by several factors including storage temperature and the presence of non-starch components (such as polysaccharide). In this study, the effects of the endogenous yam non-starch polysaccharide (YNSP) with different concentrations on the yam starch (YS) retrogradation behavior were investigated by asymmetrical flow field-flow fractionation (AF4), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Furthermore, the effects of isothermal and temperature-cycled storage on the retrogradation of YNSP + YS blend were systematically studied. An effect mechanism of the endogenous YNSP on the YS retrogradation during temperature-cycled storage was proposed. The results revealed that 4/-20 °C temperature-cycled storage facilitated YS retrogradation, owing to the formation of starch-rich regions during frozen storage. The mechanical squeezing force caused by the size growth of ice crystal during long-term frozen storage encouraged starch molecules to constantly rearrange, promoting YS retrogradation. Moreover, the YNSP can not only interact with amylose and amylopectin molecules to form aggregates that could promote YS short-term retrogradation, but also compete with amylopectin for water molecules, which might enhance the syneresis process during repeated freeze-chill storage, thereby facilitating YS retrogradation. The results suggested that a combination of AF4, FTIR, and XRD is a powerful method not only for monitoring the interaction between YNSP and YS, but also for investigating the effects of YNSP content and temperature on YS retrogradation. The present study might offer a theoretical foundation and guidance for the application of YNSP to starchy products.

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利用不对称流场-流分馏-多检测器耦合技术研究内源性非淀粉多糖对山药淀粉温度循环降解行为的影响

淀粉的降解是一个复杂的过程，受多种因素的影响，包括储存温度和非淀粉成分（如多糖）的存在。采用不对称流场-流动分选（AF4）、傅里叶变换红外光谱（FTIR）和x射线衍射（XRD）技术，研究了不同浓度的内源山药非淀粉多糖（YNSP）对山药淀粉（YS）降解行为的影响。此外，还系统地研究了等温储存和温度循环储存对YNSP + YS共混物降解的影响。提出了内源YNSP对低温循环贮藏过程中YS退化的影响机制。结果表明，4/-20°C温度循环贮藏有利于YS的退化，这是由于在冷冻贮藏过程中形成了富含淀粉的区域。在长期冷冻储存过程中，冰晶尺寸增大所产生的机械挤压力促使淀粉分子不断重排，促进了YS的降解。此外，YNSP不仅可以与直链淀粉和支链淀粉分子相互作用形成聚集体，促进YS的短期退化，而且还可以与支链淀粉竞争水分子，这可能会在重复冷冻冷藏过程中增强协同作用过程，从而促进YS的退化。结果表明，结合AF4、FTIR和XRD，不仅可以监测YNSP与YS之间的相互作用，而且可以研究YNSP含量和温度对YS降解的影响。本研究可为YNSP在淀粉类产品中的应用提供理论基础和指导。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.