Weinan Shi , Fei Fan , Jia Zhang , Lu Zhang , Guohui Xiao , Zhong Ren , Juanli Zhao , Xianghui Wang
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引用次数: 0
Abstract
Nucleic acids are genetic molecules that carry biological information, the development of accurate nucleic acid detection technology is of great significance for the treatment of some diseases. Here, we proposed a novel scheme to specific detect Let-7a (miRNA) and simultaneous discrimination from DNA based on terahertz (THz) metasurface. The metasurface is designed for supporting quasi-bound states in continuum (Q-BIC) resonance, which can effectively improve the sensing performance. Numerical simulation consistent with experimental results confirms the Q-BIC is induced by the symmetry broken between the metal wires. To specific detect trace amount Let-7a, the PBA-AuNPs probe developed by modifying Au nanoparticles (AuNPs) with phenylboronic acid (PBA), is integrated into metasurface for signal amplification. Our results show that highly sensitive and selective detection of Let-7a is achieved, the detection limit can reach to 0.01 nM. More importantly, due to the selective binding of the nanoprobe, the sensitivity for Let-7a is more than 3 times that of DNA sharing the same sequence. Even in serum samples, the frequency shift of resonance still maintains a good linear relationship with the logarithm of the Let-7a concentration. This miRNA-targeted PBA-AuNPs amplification technology offers a versatile platform with translational potential across disciplines, particularly advancing emerging THz metasurface-based biosensing.
期刊介绍:
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.