Terahertz Let-7a specific sensing and DNA differentiation enabled by Q-BIC metasurface and phenylboronic acid-gold nanoprobe

IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Weinan Shi , Fei Fan , Jia Zhang , Lu Zhang , Guohui Xiao , Zhong Ren , Juanli Zhao , Xianghui Wang
{"title":"Terahertz Let-7a specific sensing and DNA differentiation enabled by Q-BIC metasurface and phenylboronic acid-gold nanoprobe","authors":"Weinan Shi ,&nbsp;Fei Fan ,&nbsp;Jia Zhang ,&nbsp;Lu Zhang ,&nbsp;Guohui Xiao ,&nbsp;Zhong Ren ,&nbsp;Juanli Zhao ,&nbsp;Xianghui Wang","doi":"10.1016/j.talanta.2025.128577","DOIUrl":null,"url":null,"abstract":"<div><div>Nucleic acids are genetic molecules that carry biological information, the development of accurate nucleic acid detection technology is of great significance for the treatment of some diseases. Here, we proposed a novel scheme to specific detect Let-7a (miRNA) and simultaneous discrimination from DNA based on terahertz (THz) metasurface. The metasurface is designed for supporting quasi-bound states in continuum (Q-BIC) resonance, which can effectively improve the sensing performance. Numerical simulation consistent with experimental results confirms the Q-BIC is induced by the symmetry broken between the metal wires. To specific detect trace amount Let-7a, the PBA-AuNPs probe developed by modifying Au nanoparticles (AuNPs) with phenylboronic acid (PBA), is integrated into metasurface for signal amplification. Our results show that highly sensitive and selective detection of Let-7a is achieved, the detection limit can reach to 0.01 nM. More importantly, due to the selective binding of the nanoprobe, the sensitivity for Let-7a is more than 3 times that of DNA sharing the same sequence. Even in serum samples, the frequency shift of resonance still maintains a good linear relationship with the logarithm of the Let-7a concentration. This miRNA-targeted PBA-AuNPs amplification technology offers a versatile platform with translational potential across disciplines, particularly advancing emerging THz metasurface-based biosensing.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128577"},"PeriodicalIF":5.6000,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039914025010677","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Nucleic acids are genetic molecules that carry biological information, the development of accurate nucleic acid detection technology is of great significance for the treatment of some diseases. Here, we proposed a novel scheme to specific detect Let-7a (miRNA) and simultaneous discrimination from DNA based on terahertz (THz) metasurface. The metasurface is designed for supporting quasi-bound states in continuum (Q-BIC) resonance, which can effectively improve the sensing performance. Numerical simulation consistent with experimental results confirms the Q-BIC is induced by the symmetry broken between the metal wires. To specific detect trace amount Let-7a, the PBA-AuNPs probe developed by modifying Au nanoparticles (AuNPs) with phenylboronic acid (PBA), is integrated into metasurface for signal amplification. Our results show that highly sensitive and selective detection of Let-7a is achieved, the detection limit can reach to 0.01 nM. More importantly, due to the selective binding of the nanoprobe, the sensitivity for Let-7a is more than 3 times that of DNA sharing the same sequence. Even in serum samples, the frequency shift of resonance still maintains a good linear relationship with the logarithm of the Let-7a concentration. This miRNA-targeted PBA-AuNPs amplification technology offers a versatile platform with translational potential across disciplines, particularly advancing emerging THz metasurface-based biosensing.

Abstract Image

Q-BIC超表面和苯基硼酸-金纳米探针实现太赫兹Let-7a特异性传感和DNA分化
核酸是携带生物信息的遗传分子,发展准确的核酸检测技术对某些疾病的治疗具有重要意义。在此,我们提出了一种基于太赫兹(THz)超表面特异性检测Let-7a (miRNA)并同时与DNA区分的新方案。该超表面用于支持连续共振(Q-BIC)中的准束缚态,可以有效地提高传感性能。数值模拟结果与实验结果一致,证实了Q-BIC是由金属丝之间的对称性破坏引起的。为了特异检测微量Let-7a,将苯基硼酸(PBA)修饰金纳米粒子(AuNPs)制成的PBA-AuNPs探针集成到超表面上进行信号放大。结果表明,该方法对Let-7a具有较高的灵敏度和选择性,检测限可达0.01 nM。更重要的是,由于纳米探针的选择性结合,对Let-7a的敏感性是具有相同序列的DNA的3倍以上。即使在血清样品中,共振频移仍与Let-7a浓度的对数保持良好的线性关系。这种靶向mirna的PBA-AuNPs扩增技术提供了一个多功能平台,具有跨学科的转化潜力,特别是促进了新兴的基于太赫兹超表面的生物传感。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Talanta
Talanta 化学-分析化学
CiteScore
12.30
自引率
4.90%
发文量
861
审稿时长
29 days
期刊介绍: Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信