Enhanced peroxidase-like activity of single-atom dispersed cobalt in carbon nitride for glutathione detection via chemiluminescence signals of non-luminol system

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-07-12 DOI:10.1016/j.talanta.2025.128580

Zhiqiang Wang , Suxing Jiao , Yingzi Liu, Xulin Liu, Jing Wu

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引用次数: 0

Abstract

Chemiluminescence (CL) analysis is regarded as a significant analytical tool due to its advantages, including no background interference, high sensitivity, easy operation, and cost-effectiveness. However, low emission intensity hinders its wide application. Therefore, it is necessary to develop sensitizers to enhance CL performance. In this work, one kind of single-atom catalysts (SACs) was prepared by atomically dispersing Co atoms on carbon nitride (Co–C₃N₄ SACs) and applied in enhancing the CL intensity of NaHSO₃–H₂O₂ system for 107 times. The mechanism for CL enhancement of Co–C₃N₄ SACs was studied by systematic experiments and theoretic calculation. Co–C₃N₄ SACs exhibited strong peroxidase-like activity in H₂O₂ decomposition, generating reactive oxygen species (ROS) as the key step for CL signal enhancement. The constructed Co–C₃N₄ SACs-NaHSO₃-H₂O₂ CL system was utilized for the detection of glutathione (GSH), demonstrating a linear range of 10 μM–500 μM and a good linear relationship (R² = 0.9978). This approach was successfully applied in detecting GSH in human serum, demonstrating excellent recoveries and repeatability. Our work has extended the application of SACs in non-luminol CL system and explored their capability in developing CL sensors.

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增强氮化碳中单原子分散钴过氧化物酶样活性的非发光系统化学发光信号检测谷胱甘肽

化学发光分析以其无背景干扰、灵敏度高、操作简便、经济高效等优点被认为是一种重要的分析工具。然而，低排放强度阻碍了其广泛应用。因此，有必要开发增敏剂来提高CL性能。本文将Co原子原子分散在氮化碳（Co - c3n4 SACs）上，制备了一种单原子催化剂（SACs），并将其用于提高NaHSO3-H2O2体系CL强度107倍。通过系统的实验和理论计算，研究了Co-C3N4 SACs的CL增强机理。Co-C3N4 SACs在H2O2分解中表现出较强的过氧化物酶样活性，产生活性氧（ROS）是CL信号增强的关键步骤。构建的Co-C3N4 SACs-NaHSO3-H2O2 CL体系用于谷胱甘肽（GSH）的检测，线性范围为10 μM - 500 μM，线性关系良好（R2 = 0.9978）。该方法已成功应用于人血清中谷胱甘肽的检测，具有良好的回收率和重复性。我们的工作扩展了SACs在非鲁米诺CL系统中的应用，并探索了SACs在CL传感器中的应用能力。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.