IF 6.1 1区化学

Talanta Pub Date : 2025-07-29 DOI: 10.1016/j.talanta.2025.128641

Zahira A. Contreras-Atrisco , Camila S. Gómez-Navarro , Sofia Cornejo-León , Rocío Fonseca-Aguiñaga , Walter M. Warren-Vega , Ana I. Zárate-Guzmán , Luis A. Romero-Cano

{"title":"Green electrochemical detection of copper in alcoholic beverages using agave waste electrodes and digital twin integration","authors":"Zahira A. Contreras-Atrisco , Camila S. Gómez-Navarro , Sofia Cornejo-León , Rocío Fonseca-Aguiñaga , Walter M. Warren-Vega , Ana I. Zárate-Guzmán , Luis A. Romero-Cano","doi":"10.1016/j.talanta.2025.128641","DOIUrl":"10.1016/j.talanta.2025.128641","url":null,"abstract":"<div><div>The sustainable monitoring of trace metals in food and beverage production is critical for ensuring product safety, regulatory compliance, and minimizing the environmental footprint of conventional analytical methods. However, widespread adoption of electrochemical sensing in industrial settings remains limited by the reliance on non-renewable materials, high instrumentation costs, and limited integration with digital control systems. In this study, we address these challenges by developing low-cost, agave bagasse-derived activated carbon (S<sub>BET</sub> = 873 m<sup>2</sup> g<sup>−1</sup>, –COOH functionalized) as a renewable material for carbon paste electrodes (20 % activated carbon, 80 % graphite). The resulting sensor exhibited an electroactive area of 6.69 × 10<sup>−4</sup> cm<sup>2</sup> and enabled sensitive copper detection at 0.0 V vs. Ag/AgCl<sub>(sat)</sub>, with a quantification limit of 60 μg L<sup>−1</sup> and a linear range of 60–2000 μg L<sup>−1</sup>. Performance was statistically comparable to atomic absorption spectroscopy in real Tequila and Mezcal samples. To further enhance applicability, the sensor was embedded into a MATLAB-Simulink digital twin prototype for real-time simulation, predictive monitoring, and automated alerts. This work not only valorizes a major agroindustrial waste stream but also presents a digitally enabled, eco-efficient alternative to conventional techniques—reducing chemical use, energy demands, and equipment complexity—while supporting sustainable quality control in agave spirit manufacturing.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128641"},"PeriodicalIF":6.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MnO2 nanozyme-based ratiometric fluorescent nanoplatform for glutathione detection and intracellular imaging 基于MnO2纳米酶的比例荧光纳米平台用于谷胱甘肽检测和细胞内成像

IF 6.1 1区化学

Talanta Pub Date : 2025-07-28 DOI: 10.1016/j.talanta.2025.128645

Lin Chai , Haoyu Chen , Xing Yang , Jing Liu , Qiquan Yang , Shu Huang , Min Tang , Xiaohua Zhu , Haitao Li , Youyu Zhang , Meiling Liu

{"title":"MnO2 nanozyme-based ratiometric fluorescent nanoplatform for glutathione detection and intracellular imaging","authors":"Lin Chai , Haoyu Chen , Xing Yang , Jing Liu , Qiquan Yang , Shu Huang , Min Tang , Xiaohua Zhu , Haitao Li , Youyu Zhang , Meiling Liu","doi":"10.1016/j.talanta.2025.128645","DOIUrl":"10.1016/j.talanta.2025.128645","url":null,"abstract":"<div><div>Ratiometric fluorescent nanoplatforms with self-calibrating capabilities offer enhanced detection accuracy by minimizing environmental interference. However, challenges such as signal cross-interference and nanomaterial instability persist in complex biological systems. To address these issues, we developed a glutathione (GSH)-responsive ratiometric fluorescent nanoprobe by integrating a porphyrin-based metal-organic framework (PCN-224) with MnO<sub>2</sub> nanosheets (NSs). The PCN-224 serves as both a nanocarrier and luminescent moiety, while MnO<sub>2</sub> acts as oxidase (OXD) mimetic nanozyme and the reactive site for GSH. The ratiometric detection mechanism relies on the intrinsic fluorescence of PCN-224 (emission at 655 nm) and the OXD-like activity of PCN-224@MnO<sub>2</sub>, which catalyzes the oxidation of <em>o</em>-phenylenediamine (OPD) to generate fluorescent 2,3-diaminophenazine (DAP, emission at 570 nm). Upon GSH exposure, the redox reaction between GSH and MnO<sub>2</sub> produces Mn<sup>2+</sup>, quenching the OXD-like activity and diminishing the DAP fluorescence, while the PCN-224 fluorescence recovers due to MnO<sub>2</sub> decomposition. The nanoplatform exhibits a wide detection range spanning from 0.1 to 60 μM with a low detection limit of 15 nM and high selectivity. Furthermore, it enables accurate GSH quantification in complex biological matrices, including human serum and cell lysates, and facilitates real-time monitoring of endogenous and exogenous GSH in living cells via fluorescence imaging. This study not only presents sensitive and reliable platform for GSH detection, but also overcomes key limitations in ratiometric sensing, such as signal interference and nanomaterial instability, by utilizing a stimuli-responsive fluorescent nanoprobe design.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128645"},"PeriodicalIF":6.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast visualization of multiple trace antibiotics by modified Eu/Tb MOF test paper 改良Eu/Tb MOF试纸快速可视化多种微量抗生素

IF 6.1 1区化学

Talanta Pub Date : 2025-07-28 DOI: 10.1016/j.talanta.2025.128649

Fang Yuan, Dingfan Yan, Shuaibo Song, Jinbo Zhang, Zheng Chen, Jiahao Lu, Simin Wang, Tao Li

{"title":"Fast visualization of multiple trace antibiotics by modified Eu/Tb MOF test paper","authors":"Fang Yuan, Dingfan Yan, Shuaibo Song, Jinbo Zhang, Zheng Chen, Jiahao Lu, Simin Wang, Tao Li","doi":"10.1016/j.talanta.2025.128649","DOIUrl":"10.1016/j.talanta.2025.128649","url":null,"abstract":"<div><div>To address the current situation of the poor detection efficiency of multiple trace antibiotic contamination in the aqueous environment, we synthesized a modified lanthanide MOF material, Ln-MOF(NH<sub>2</sub>)@R6G, for the rapid visual quantitation of trace norfloxacin (NOR), ciprofloxacin (CIP) and tetracycline (TC). By introducing the ligands of NH<sub>2</sub>-BDC and R6G with abundant amino groups, the interaction of material with antibiotics was largely strengthened. Sensitive detection completed within 1 min with dramatically low detection limit of LOD<sub>NOR</sub> = 16.56 nM, LOD<sub>CIP</sub> = 6.14 nM, LOD<sub>TC</sub> = 19.6 nM. Based on the visible color change to the naked eyes, we succeeded in loading the synthesized material onto the test paper, which realized semi-quantitative detection of antibiotics (concentration range of 10–100 μM) in actual water systems. Taking advantages of the significant difference of the NOR and CIP induced fluorescence color, as well as the non-interfering property with each other, we developed a data model for simultaneously visual quantifying NOR and CIP by the RGB-CIE variation maps in their coexisting actual system (recoveries 96.48 %–105.34 %, RSD 0.03 %–0.81 %). The result developed a promising portable visual analysis for the trace antibiotics in complex waters, and provided a novel strategy for in situ screening of environmental pollutants.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128649"},"PeriodicalIF":6.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High sensitive optical-chemical sensors based on optical fibers in a reflection scheme combined with C-shaped waveguides of MIP-microbeads 基于反射方案的光纤与mip微珠c形波导结合的高灵敏度光化学传感器

IF 6.1 1区化学

Talanta Pub Date : 2025-07-28 DOI: 10.1016/j.talanta.2025.128647

Rosalba Pitruzzella , Filipa Sequeira , Alessandra Cutaia , Chiara Marzano , Francesco Arcadio , Catarina Cardoso Novo , Ricardo Oliveira , Maria Pesavento , Luigi Zeni , Giancarla Alberti , Rogerio Nunes Nogueira , Nunzio Cennamo

{"title":"High sensitive optical-chemical sensors based on optical fibers in a reflection scheme combined with C-shaped waveguides of MIP-microbeads","authors":"Rosalba Pitruzzella , Filipa Sequeira , Alessandra Cutaia , Chiara Marzano , Francesco Arcadio , Catarina Cardoso Novo , Ricardo Oliveira , Maria Pesavento , Luigi Zeni , Giancarla Alberti , Rogerio Nunes Nogueira , Nunzio Cennamo","doi":"10.1016/j.talanta.2025.128647","DOIUrl":"10.1016/j.talanta.2025.128647","url":null,"abstract":"<div><div>In order to monitor analytes in liquid matrices, optical fiber probes in reflection mode can be exploited in several application fields. This work presents a novel sensing approach based on C-shaped waveguides obtained by filling PVC C-shaped channels with microbeads of molecularly imprinted polymers (MIP-microbeads). Specifically, for the first time, MIP microbeads are used as a sensitive core of optical waveguides with several advantages. The MIP microbeads C-shaped waveguide is in contact with a polished plastic optical fiber (POF) at one end to connect the sensitive region with the light source and the detector in a reflection-based scheme via a simple POF-based splitter. As a proof of concept, the MIP-microbeads were prepared using 2-furaldehyde (2-FAL) as the template to compare the results with other optical fiber sensors. Moreover, to highlight the performance improvement of the proposed sensing approach compared to MIP-based configurations, a sensor based on an MIP (MIP-mass sensor) instead of MIP-microbeads has been developed, tested, and compared with the MIP-microbeads sensor and the state-of-the-art.</div><div>The results obtained via the MIP-microbeads sensor demonstrated the best performance, with an ultra-low detection limit at a pico-nano molar level and an ultra-wide detection concentration range of about four orders of magnitude. Moreover, the proposed optical-chemical sensor probe is small-size (at a micron scale), simple to realize, low-cost, mechanically robust, suitable for installation even in extreme conditions, and can be used in a real scenario where a reflection scheme sensor is required. An experimental test in the food application field is reported.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128647"},"PeriodicalIF":6.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Membrane-based microfluidic chip constructed and applied in magnetic microparticle anchoring-and-release digital immunoassay 膜基微流控芯片的构建及其在磁微粒锚定释放数字免疫分析中的应用

IF 6.1 1区化学

Talanta Pub Date : 2025-07-28 DOI: 10.1016/j.talanta.2025.128644

Songbai Tian , Tingting Xiang , Xinghu Ji , Fuxiang Zhou , Zhike He

{"title":"Membrane-based microfluidic chip constructed and applied in magnetic microparticle anchoring-and-release digital immunoassay","authors":"Songbai Tian , Tingting Xiang , Xinghu Ji , Fuxiang Zhou , Zhike He","doi":"10.1016/j.talanta.2025.128644","DOIUrl":"10.1016/j.talanta.2025.128644","url":null,"abstract":"<div><div>Digital immunoassay is a valuable method for quantifying protein at the single-molecule level. Traditional digital immunoassay (the single-molecule arrays, SiMoAs) involves distributing the antibody-antigen-antibody sandwich immune complex into droplets, but some targets are lost during this process. To address this issue, we developed a magnetic microparticle anchoring-and-release digital immunoassay method for protein detection. We embedded a commercial track-etched polycarbonate (PCTE) membrane into the microfluidic chip for droplet generation, and modified the magnetic microparticles (MMPs) with enzymes for signal production. This method involves two processes: first, the enzymes modified MMPs are anchored in 96-well plates through the target protein. Second, the enzymes modified MMPs were released, and digitally quantified in the membrane-based microfluidic chip. This magnetic microparticle anchoring-and-released digital immunoassay method was successfully applied to the detection of p24 protein. Results showed that p24 could be digitally detected within the range of 1–100 pg/mL with good selectivity and accuracy using this digital method. By reducing signal loss during the digital immunoassay process, this method represents a significant advancement in digital technology.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128644"},"PeriodicalIF":6.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of spectral variability encountered during FTIR analysis of fresh urine 在新鲜尿液的FTIR分析中遇到的光谱变异性的评估

IF 6.1 1区化学

Talanta Pub Date : 2025-07-28 DOI: 10.1016/j.talanta.2025.128650

Elie Sarkees , Vincent Vuiblet , Olivier Piot

{"title":"Assessment of spectral variability encountered during FTIR analysis of fresh urine","authors":"Elie Sarkees , Vincent Vuiblet , Olivier Piot","doi":"10.1016/j.talanta.2025.128650","DOIUrl":"10.1016/j.talanta.2025.128650","url":null,"abstract":"<div><div>Vibrational techniques, particularly infrared absorption spectroscopy, are promising tools for routine diagnosis due to their high chemical specificity, enabling the detection of disease-associated molecular changes. Urine is a valuable biofluid for identifying diagnostic biomarkers, as its molecular composition reflects the body's pathophysiological state, particularly that of the urinary system. However, the high variability of urine complicates the identification of spectroscopic markers. This variability arises not only from biological variations but also from experimental parameters within the analytical workflow. The objective of this study was to assess the impact of specific experimental parameters on the infrared signal, particularly two key time intervals: T1, from urine emission to the onset of sample drying, and T2, from the end of drying to spectral acquisition. After assigning infrared bands to urine's major urine components (urea, creatinine and uric acid), we analysed the spectral variability associated with these experimental parameters using principal component analysis on samples from healthy volunteers. Our results showed that infrared spectral profiles were strongly influenced by urine pH. Spectral changes were greater during T1, when urine remains in liquid form, while T2 had a comparatively smaller impact. Our study led to some recommendations for urine spectral analysis to minimise variability.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128650"},"PeriodicalIF":6.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanobacteria as generators of reactive oxygen species – A review of analysis methods and case studies 作为活性氧产生菌的蓝藻-分析方法和案例研究综述

IF 6.1 1区化学

Talanta Pub Date : 2025-07-28 DOI: 10.1016/j.talanta.2025.128648

Hanna Lis, Klaudia Kwidzińska, Katarzyna Bethke, Magda Caban

引用次数: 0

Palindrome design-guided “single-flap-double-duties” and “dual-enzymes-quadruple-cycles” enabled nano-signal amplification for customized FEN1 sensing 回文设计导向的“单瓣-双职责”和“双酶-四周期”纳米信号放大，用于定制FEN1传感

IF 6.1 1区化学

Talanta Pub Date : 2025-07-26 DOI: 10.1016/j.talanta.2025.128642

Jintao Chen , Yuhui Shang , Yu Yang, Yali Liu, Jinyang Chen

{"title":"Palindrome design-guided “single-flap-double-duties” and “dual-enzymes-quadruple-cycles” enabled nano-signal amplification for customized FEN1 sensing","authors":"Jintao Chen , Yuhui Shang , Yu Yang, Yali Liu, Jinyang Chen","doi":"10.1016/j.talanta.2025.128642","DOIUrl":"10.1016/j.talanta.2025.128642","url":null,"abstract":"<div><div>Flap endonuclease 1 (FEN1), a structure-specific nuclease, is usually overexpressed in various types of cancer cells and has been recognized as a promising biomarker for molecular diagnostics. In this work, we developed a one-tube and ultrasensitive method for FEN1 sensing based on customized target recognition, exponential signal amplification and nano-signal transduction. Due to rational palindrome design, only two DNA substrates and a pair of enzymes were needed in the sensing. Within the dumbbell DNA probe, the palindromic sequence was divided into two parts. One was designed as 5′-end overhanging flap that can trigger signal amplification in presence of FEN1 and prevent signal output in absence of FEN1. And the other one was complementary sequence of 5′ flap which is available for initiating quadruple isothermal-amplification cycles assisted with a palindromic hairpin and driven by polymerase and endonuclease. The seamless integration of “single-flap-double-duties” with “dual-enzymes-quadruple-cycles” thus enabled exponential signal amplification of fluorescent copper nanoparticles and contributed to ultrasensitive FEN1 sensing with a detection limit of 3.5 × 10<sup>−6</sup> U/mL. Thanks to its high selectivity and anti-interference ability, this method was able to unambiguously distinguish cancer cells from normal cell based on the test results of cellular FEN1. This work is expected to provide a promising strategy of cancer biomarker detection for advanced molecular diagnostics.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128642"},"PeriodicalIF":6.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AIEgen-based metal-organic gels for rapid and ultrasensitive discrimination of piceatannol from core-isostructural analogues 基于aiegen的金属-有机凝胶用于快速和超灵敏地区分皮杉酚与核心同工结构类似物

IF 5.6 1区化学

Talanta Pub Date : 2025-07-25 DOI: 10.1016/j.talanta.2025.128637

Huifeng Xu , Rui Pan , Weihua Huang , Shufei Huang , Ronglin Chen , Lili Wang , Xiao Hu , Lishuang Yu , Qi Li , Xi Zhu , Xihai Li

{"title":"AIEgen-based metal-organic gels for rapid and ultrasensitive discrimination of piceatannol from core-isostructural analogues","authors":"Huifeng Xu , Rui Pan , Weihua Huang , Shufei Huang , Ronglin Chen , Lili Wang , Xiao Hu , Lishuang Yu , Qi Li , Xi Zhu , Xihai Li","doi":"10.1016/j.talanta.2025.128637","DOIUrl":"10.1016/j.talanta.2025.128637","url":null,"abstract":"<div><div>A highly stable and strongly fluorescent metal-organic gel, Zr-TCBPE-MOG, was successfully synthesized through the combination of aggregation-induced emission luminogens (AIEgens) 1, 1, 2, 2-tetra(4-carboxylbiphenyl) ethylene (H<sub>4</sub>TCBPE) with Zr (IV) cations. The fluorescence of Zr-TCBPE-MOG exhibits a sensitive response to piceatannol (3,3′,4,5′-tetrahydroxy <em>trans</em>-stilbene, PCT), whereas its structural analogues, such as resveratrol, pterostilbene, rhapontigenin, and pinosylvin, which share the same core skeleton, have no effect on it. Therefore, Zr-TCBPE-MOG can function as a fluorescent probe for rapid differentiation and detection of PCT among these structural analogues. A simple and rapid fluorescence method was established via the selective fluorescence quenching effect of PCT on Zr-TCBPE-MOG through the photoinduced electron transfer effect. Under optimal measurement conditions, the proposed method achieves high sensitivity for PCT with a linear detection concentration range from 3.5 to 250 nM and a low detection limit of 2.8 nM. It also can realize selective determination of PCT in serum samples. This work reveals the great potential of MOG for the rapid and highly specific differentiation of structurally similar drugs.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128637"},"PeriodicalIF":5.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a sensitive assay for analysis of D/L-serine in cells using ultra-high performance liquid chromatography-fluorescence detector 利用高效液相色谱-荧光检测器建立和验证细胞中D/ l -丝氨酸的灵敏分析方法

IF 5.6 1区化学

Talanta Pub Date : 2025-07-25 DOI: 10.1016/j.talanta.2025.128634

Liang Liu , Shiao Ren , Yangyi Hao, Lijuan Yue, Chenli Yue, Longyu Li, Wenlei Zhang, Zengliang Gao, Xin Hai

{"title":"Development and validation of a sensitive assay for analysis of D/L-serine in cells using ultra-high performance liquid chromatography-fluorescence detector","authors":"Liang Liu , Shiao Ren , Yangyi Hao, Lijuan Yue, Chenli Yue, Longyu Li, Wenlei Zhang, Zengliang Gao, Xin Hai","doi":"10.1016/j.talanta.2025.128634","DOIUrl":"10.1016/j.talanta.2025.128634","url":null,"abstract":"<div><div>The aim of this study was to establish a simple, sensitive, and robust ultra-high performance liquid chromatography coupled with fluorescence detection method (UPLC-FLD) for the determination of chiral D/L-serine in cells. D/L-serine in cells were derivatizated by o-phthalaldehyde (OPA) and N-acetyl-L-cysteine (NAC). Carbocisteine was selected as the internal standard. The derivatives were separated on a C18 column by gradient elution. The excitation and emission wavelengths for fluorescence determination are 340 nm and 450 nm, respectively. The retention time of D-serine and L-serine was 23.3 and 23.9 min respectively, which presented a perfect separation. The accuracy of D-serine and L-serine were ranged from 96.46 % to 109.63 % and 95.50 %–102.20 %, respectively. The precision of D-serine and L-serine were ranged from 4.34 % to 14.56 % and 3.56 %–13.73 %. The limit of quantitation of D-serine and L-serine were 0.1 nmol/mL. The concentration of D/L-serine varies in different cell lines. This method can satisfy the determination of D/L-serine in astrocytes and other cells, which can be used for the study of D/L-serine metabolism and related mechanisms. In short, we have established a simple, stable, reliable and robust method for the determination of D/L-serine in cells. In addition, the D/L-serine levels in different cells were quantified in this study, which can provide a reference for the study of D/L-serine metabolism in cells.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128634"},"PeriodicalIF":5.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Talanta最新文献