Talanta最新文献_第7页

The viscosity response behavior of bare nanopipettes based on ionic current rectification

IF 5.6 1区化学

Talanta Pub Date : 2025-04-10 DOI: 10.1016/j.talanta.2025.128111

Yue-Qian Zhu , Yu-Chen Wu , Xu-Fan Wang , Jun-Hao Zhai , Xin-Yue Zhang , Xin-Qian Xu , Yu-Xuan Ma , Meng-Liu Sha , Xing-Yu Bai , Zi-Jing Liu , Wen-Yi Peng , Shen Sun , Guo-Chang Liu

{"title":"The viscosity response behavior of bare nanopipettes based on ionic current rectification","authors":"Yue-Qian Zhu , Yu-Chen Wu , Xu-Fan Wang , Jun-Hao Zhai , Xin-Yue Zhang , Xin-Qian Xu , Yu-Xuan Ma , Meng-Liu Sha , Xing-Yu Bai , Zi-Jing Liu , Wen-Yi Peng , Shen Sun , Guo-Chang Liu","doi":"10.1016/j.talanta.2025.128111","DOIUrl":"10.1016/j.talanta.2025.128111","url":null,"abstract":"<div><div>In this paper, bare nanopipettes perfused with low-viscosity electrolyte solutions achieved ionic current rectification (ICR) response capabilities to solution viscosity, providing a simple method for fabricating nanopipette viscosity probes. The ICR phenomenon was investigated in four different viscosity configurations and the viscosity response mechanism of the nanopipette was explained by the effect of the electric double layer and electroosmotic flow on the conductivity of the nanopipettes’ tip. The viscosity response sensitivity was improved by optimizing influencing factors such as KCl concentration, pH, and nanopipette diameter. Data fitting showed that within the viscosity range from 0.91 mPa.s to 40.07 mPa.s, the rectification ratio of the nanopipettes has a good linear relationship with the solution viscosity (<em>r = 0.04η - 0.02</em>). Additionally, the bare nanopipettes exhibit good reversibility and selectivity in viscosity response and also demonstrate the potential for use in single-cell cytoplasmic viscosity detection. The viscosity response behavior of bare nanopipettes provides important insights for constructing cytoplasmic viscosity probes, which will expand the applications of nanopipettes in cytoplasmic detection.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128111"},"PeriodicalIF":5.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loading of hyaluronic acid–conjugated MoS2 nanosheets with rhodamine B for sensing hyaluronidase activity in human urine and live cells

IF 5.6 1区化学

Talanta Pub Date : 2025-04-10 DOI: 10.1016/j.talanta.2025.128110

Aravindan Varatharajan , Chia-Chi Chang , Wei–Lung Tseng

{"title":"Loading of hyaluronic acid–conjugated MoS2 nanosheets with rhodamine B for sensing hyaluronidase activity in human urine and live cells","authors":"Aravindan Varatharajan , Chia-Chi Chang , Wei–Lung Tseng","doi":"10.1016/j.talanta.2025.128110","DOIUrl":"10.1016/j.talanta.2025.128110","url":null,"abstract":"<div><div>Efficient molecule loading onto nanomaterials is a key step for advanced biosensing and imaging because of improved sensitivity and enhanced targeting of the resultant nanoprobe. In contrast to other nanomaterials, MoS<sub>2</sub> nanosheets (NSs) possess unique advantageous of large surface area and abundant sulfur defects. Herein, we explored the conjugation of MoS<sub>2</sub> NSs with thiol-conjugated hyaluronic acid (HA-SH) for selective detection of hyaluronidase (HAase) and specific binding to CD44 receptor-positive cancer cells. Rhodamine B (RhB) molecules were electrostatically and hydrophobically coupled to the HA–SH–MoS<sub>2</sub> NS surface with a binding constant of 7.14 × 10<sup>16</sup> M<sup>−1</sup>. The HA–SH–MoS<sub>2</sub> NS-mediated fluorescence quenching of the adsorbed RhB molecules proceeds <em>via</em> a combination of static and dynamic quenching in the ratio of 10:1. Upon the addition of HAase, the adsorbed RhB molecules are liberated from the RB-HA–SH–MoS<sub>2</sub> NSs <em>via</em> HAase-mediated hydrolysis of HA-SH. As a result, the restored fluorescence of RhB molecules is proportional to the HAase concentration. The RhB-HA–SH–MoS<sub>2</sub> NSs achieve a detection limit of 0.37 U/mL for HAase with a linear response range from 0.05 to 4.5 U/mL and satisfactory selectivity against potential interferents. The RhB-HA–SH–MoS<sub>2</sub> NSs not only effectively quantify HAase in urine sample but also enable selective imaging of intracellular HAase activity through CD44 receptor-mediated endocytosis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128110"},"PeriodicalIF":5.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence-activated cell sorting-based efficient screening of monensin monoclonal antibodies and applications in lateral flow immunoassay

IF 5.6 1区化学

Talanta Pub Date : 2025-04-10 DOI: 10.1016/j.talanta.2025.128128

Jingjie Huang , Ao Lin , Yani Gu , Xiaole Pan , XinXin Ma , Yanzhe Qing , Jiancheng Li

{"title":"Fluorescence-activated cell sorting-based efficient screening of monensin monoclonal antibodies and applications in lateral flow immunoassay","authors":"Jingjie Huang , Ao Lin , Yani Gu , Xiaole Pan , XinXin Ma , Yanzhe Qing , Jiancheng Li","doi":"10.1016/j.talanta.2025.128128","DOIUrl":"10.1016/j.talanta.2025.128128","url":null,"abstract":"<div><div>Monensin is widely used in livestock and poultry for disease prevention and growth promotion. Still, its improper use can lead to residues in animal-derived foods, posing risks to human health. In immunoassays, monoclonal antibodies (mAbs) remain a preferred choice due to their high sensitivity and specificity. However, traditional hybridoma technology often suffers from a lengthy screening cycle and the risk of losing effective clones. To address these limitations, this study employed fluorescently labeled monensin antigens combined with fluorescence-activated cell sorting (FACS) for rapid screening of hybridoma cells secreting anti-monensin mAbs. Compared to the conventional limiting dilution method, this approach increased the yield of specific hybridoma cells by tenfold and reduced the screening cycle from four weeks to one week. Based on the selected mAb (4E6), a rapid visual lateral flow immunoassay (LFIA) was developed for on-site detection of monensin, with a total detection time of 10 min. The assay exhibited a half-maximal inhibitory concentration (IC<sub>50</sub>) of 2.32 μg/kg and a linear detection range of 0.32–10.3 μg/kg. This study provides a promising strategy for efficient hybridoma cell screening and practical monitoring of monensin residues in environmental and food samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128128"},"PeriodicalIF":5.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructing amino and hydroxyl group co-modified porous organic polymers for the efficient enrichment of phenoxyacetic acid herbicides and their determination by HPLC-UV

IF 5.6 1区化学

Talanta Pub Date : 2025-04-09 DOI: 10.1016/j.talanta.2025.128120

Yiran Dou , Ying Zhao , Lin Hao , Qianqian Wang , Qiuhua Wu , Chun Wang

{"title":"Constructing amino and hydroxyl group co-modified porous organic polymers for the efficient enrichment of phenoxyacetic acid herbicides and their determination by HPLC-UV","authors":"Yiran Dou , Ying Zhao , Lin Hao , Qianqian Wang , Qiuhua Wu , Chun Wang","doi":"10.1016/j.talanta.2025.128120","DOIUrl":"10.1016/j.talanta.2025.128120","url":null,"abstract":"<div><div>As hormone-type herbicides, phenoxyacetic acid herbicides (PAAs) residues in environment and food pose severe risks to human health. In this study, a hydroxyl and amino modified azo-linked porous polymer (NH-OH-POP) was synthesized for the first time. As a solid-phase extraction (SPE) adsorbent, NH-OH-POP achieved efficient enrichment of PAAs across a broad pH range (pH 1–9), far surpassing the pH range of all previously reported adsorbents. With NH-OH-POP as SPE adsorbent, a highly efficient high-performance liquid chromatography method for the analysis of trace PAAs in real water and vegetable samples has been established. The method demonstrated good linearity (determination coefficient >0.998), high sensitivity (detection limits of 0.08–0.30 ng mL<sup>−1</sup> for water, 6.00–18.0 ng g<sup>−1</sup> for wax gourd, and 6.50–20.0 ng g<sup>−1</sup> for cucumber), and high recovery rates (87.4–107 %), with relative standard deviations below 7.2 %. This work can shed light on constructing multifunctional POPs for the robust enrichment and detection of acidic organic pollutants.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128120"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and simple sensing of acetylcholinesterase and inhibition activity by utilizing a portable Raman spectrometer

IF 5.6 1区化学

Talanta Pub Date : 2025-04-09 DOI: 10.1016/j.talanta.2025.128086

Jing Tang , Jinyue Feng , Huanhua Liang , Yilan Pang , Zhijiao Tang , Zhengyi Chen , Jian Liang , Yumin Wang

{"title":"Rapid and simple sensing of acetylcholinesterase and inhibition activity by utilizing a portable Raman spectrometer","authors":"Jing Tang , Jinyue Feng , Huanhua Liang , Yilan Pang , Zhijiao Tang , Zhengyi Chen , Jian Liang , Yumin Wang","doi":"10.1016/j.talanta.2025.128086","DOIUrl":"10.1016/j.talanta.2025.128086","url":null,"abstract":"<div><div>The establishment of a fast, simple-yet-practical, cost-effective and reliable sensing method for the detection of acetylcholinesterase (AChE) activity and inhibition is always desired in clinical Alzheimer's disease (AD) diagnosis and drug screening. Herein, a CoOOH nanosheet-isolated SERS nanoprobe (Ag–Au NPs@4-MBA@CoOOH, AAMC) with core-shell-molecule-shell structure was developed for sensitive and selective quantification of AChE. Experimental results indicated that the CoOOH shell can effectively impede the penetration of the external illuminated laser and block the internal SERS signal of Raman molecules. When AChE and its substrate were present, the specific AChE-catalyzed reaction would be rapidly triggered, resulting in the decomposition of CoOOH in AAMC probe and the generation of greatly enhanced SERS signal. By taking advantage of a portable Raman spectrometer, the AAMC nanoprobe is capable for rapid, sensitive and specific detection of AChE in the range 1 × 10<sup>−5</sup> - 10 U/mL (LOD of 7.9 × 10<sup>−6</sup> U/mL). Moreover, the measurement of AChE activity in complex human serum samples (with recoveries ranging from 98.0 to 103.3 %) and effective detection of its inhibition activity with the developed strategy were also successfully realized, showing great promise for on-site and point-of-care testing.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128086"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-capacitance air-brushed electrodes for capacitive label-free bioassays

IF 5.6 1区化学

Talanta Pub Date : 2025-04-09 DOI: 10.1016/j.talanta.2025.128118

Suchanat Boonkaew , Steven Linfield , Elena E. Ferapontova

{"title":"High-capacitance air-brushed electrodes for capacitive label-free bioassays","authors":"Suchanat Boonkaew , Steven Linfield , Elena E. Ferapontova","doi":"10.1016/j.talanta.2025.128118","DOIUrl":"10.1016/j.talanta.2025.128118","url":null,"abstract":"<div><div>Non-invasive assays for protein biomarkers of cancer allow both its early diagnosis and continuous treatment monitoring. Yet, accurate point-of-care (POC) diagnostic devices for cancer diagnosis and monitoring, needed in point-of-care (POC) sites and places with limited resources, are scarce, not the least, due to their high current cost or bulky equipment necessary for analysis. Here, we show that the capacitive cellulase-linked <em>electrochemical enzyme-linked aptamer-sorbent assay</em> (e-ELASA) on magnetic beads (MBs) performed with airbrushed graphite (Gr) electrodes accurately and economically detects HER-2/<em>neu</em>, the protein biomarker of some aggressive forms of cancers and target of anticancer therapy. The disposable Gr electrodes were produced by airbrushing inexpensive graphite-powder/chitosan water inks onto polyester transparency films, producing high-capacitance electrodes, whose apparent specific capacitance ranged between 3.61 and 8.88 mF cm<sup>−2</sup> as a function of the number of sprayed layers and graphite content in inks. The five-layer electrodes produced from 1.7 g of graphite powder (per 5 mL)/0.55 % chitosan water inks outperformed manually polished spectroscopic Gr electrodes earlier used in this label-free capacitive e-ELASA, as a result of the higher capacitive changes of the former, providing the same 0.1 fM limit of detection of HER-2/<em>neu</em>, in both buffer and 10 % serum, yet with a three-fold higher sensitivity. The portable and low cost airbrushed electrodes/e-ELASA set-up can be used for quick and accurate regular POC monitoring of HER-2/<em>neu</em>, particularly, in low and middle income settings, and, in perspective, the high-capacitance airbrushed electrodes can be adapted for other type label-free capacitive bioassays.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128118"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TiO2-coated honeycomb-like super-macroporous silica for high-purity extraction of exosomes from human plasma

IF 5.6 1区化学

Talanta Pub Date : 2025-04-09 DOI: 10.1016/j.talanta.2025.128098

Mengqian Jia , Chenyang Wang , Mengxi Chen , Wenzhuo Dong , Haiyang Zhang , Junjie Ou , Yinmao Wei

{"title":"TiO2-coated honeycomb-like super-macroporous silica for high-purity extraction of exosomes from human plasma","authors":"Mengqian Jia , Chenyang Wang , Mengxi Chen , Wenzhuo Dong , Haiyang Zhang , Junjie Ou , Yinmao Wei","doi":"10.1016/j.talanta.2025.128098","DOIUrl":"10.1016/j.talanta.2025.128098","url":null,"abstract":"<div><div>Screening biomarkers from exosomes has emerged as a new strategy for non-invasive early diagnosis of diseases. Nevertheless, the present screening efficiency and accuracy of biomarkers are limited by the low extraction efficiency and purity of exosomes. To address this issue, a highly selective adsorbent, which integrates size-exclusion and chemisorption, was created by coating TiO<sub>2</sub> on honeycomb-like super-macroporous silica. Cell culture medium and human plasma were employed to investigate the enrichment performance, and the results indicate that the super-macropores ranging from 63.5 to 147.5 nm together with thin pore walls allow exosomes to enter and be adsorbed by TiO<sub>2</sub> in the pores, enhancing the available surface area for exosomes meanwhile physically excluding the large-sized cell debris and vesicles. Taking advantages of these properties, the prepared adsorbent achieves a higher extraction efficiency, recovery and purity of exosomes compared with the normal adsorbents and ultracentrifugation (UC) method. Combining this method with proteomic analysis, a total of 392 proteins were identified in exosomes from healthy human plasma, which is significantly higher than the number obtained by UC (200 proteins). For clinical samples, 59 upregulated and 124 downregulated proteins were identified in the plasma from colorectal cancer (CRC) patients, of which 44 upregulated proteins and 69 downregulated proteins are strongly associated with the progression of CRC. These findings suggest that this adsorbent possesses considerable potential in the extraction of exosomes for screening biomarkers and diagnosing tumor progress.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128098"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifunctional Fe-CDs@MOF composite nanozyme based ratiometric fluorescent probe for the detection of xanthine in fish

IF 5.6 1区化学

Talanta Pub Date : 2025-04-09 DOI: 10.1016/j.talanta.2025.128108

Zhen Xie , Yuhao Gao , Tongshan Zuo , Chen Shen , Han Jiang , Guanhong Xu , Yao Cen , Jing Yang , Qin Hu , Rui Li , Fangdi Wei

{"title":"Bifunctional Fe-CDs@MOF composite nanozyme based ratiometric fluorescent probe for the detection of xanthine in fish","authors":"Zhen Xie , Yuhao Gao , Tongshan Zuo , Chen Shen , Han Jiang , Guanhong Xu , Yao Cen , Jing Yang , Qin Hu , Rui Li , Fangdi Wei","doi":"10.1016/j.talanta.2025.128108","DOIUrl":"10.1016/j.talanta.2025.128108","url":null,"abstract":"<div><div>A novel bifunctional Fe-CDs@MOF composite nanozyme was synthesized successfully by loading Fe doped carbon dots (Fe-CDs) onto metal-organic framework (MOF) MIL-101 (Fe). The introduction of Fe-CDs enhanced the peroxidase (POD)-like activity of MOF and endowed it with excellent fluorescence properties. Considering the dual functions of Fe-CDs@MOF, we combined it with <em>o</em>-phenylenediamine (OPD)/xanthine oxidase (XOD) to construct a ratiomeric fluorescence probe for xanthine (XAN) detection. XAN was first oxidized by XOD to produce H<sub>2</sub>O<sub>2</sub>, then Fe-CDs@MOF exerted its POD-like activity to catalyze the reaction between OPD and H<sub>2</sub>O<sub>2</sub> to form 2,3-diaminophenazine (DAP). DAP in turn quenched the fluorescence of Fe-CDs@MOF as a result of internal filtration effect. The ratio of the fluorescence intensity of DAP and the fluorescence intensity of Fe-CDs@MOF indicated the XAN content. The XAN detection range was 1–100 μM with a limit of detection of 0.36 μM. The ratiometric fluorescence probe was utilized to analyze XAN in fish samples, and the results obtained were in good agreement with those assayed by high performance liquid chromatography. The method also had the ability to test the freshness of frozen fish. This study provides a novel approach for the design and application of Fe-CDs@MOF composite nanozyme.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128108"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical behavior of the synthetic food diazo dye Brilliant Black BN (E151) and the first voltammetric method for its determination

IF 5.6 1区化学

Talanta Pub Date : 2025-04-09 DOI: 10.1016/j.talanta.2025.128119

Andrii Dmukhailo , Sofiia Tvorynska , Maksym Trukhym , Liliya Dubenska

{"title":"Electrochemical behavior of the synthetic food diazo dye Brilliant Black BN (E151) and the first voltammetric method for its determination","authors":"Andrii Dmukhailo , Sofiia Tvorynska , Maksym Trukhym , Liliya Dubenska","doi":"10.1016/j.talanta.2025.128119","DOIUrl":"10.1016/j.talanta.2025.128119","url":null,"abstract":"<div><div>As synthetic food azo dyes have been proven to cause adverse health problems, their content is regulated and must be strictly controlled. Therefore, the availability of reliable as well as simple and cost-effective methods for their determination is essential to ensure the quality and safety of food and beverages. This work reports on a systematic study of the electrochemical behavior of Brilliant Black BN (<em>BB BN</em>), a purple synthetic food diazo dye, labelled as E151, in aqueous media using polarography/cyclic voltammetry over a wide range of pH and scan rates. In particular, the electrochemical reduction of <em>BB BN</em> was investigated using a dropping mercury electrode, while the <em>BB BN</em> oxidation − using a glassy carbon electrode. Importantly, this work describes the development of the very first voltammetric method for the determination of <em>BB BN</em>, which is based on its reduction in the Britton-Robinson buffer, pH 3.0, at two types of silver solid amalgam electrodes (AgSAEs), such as polished (<em>p</em>-AgSAE) and mercury meniscus modified one (<em>m</em>-AgSAE). The results demonstrated that the linear scan adsorptive stripping voltammetry in combination with <em>m</em>-AgSAE allows to reach a limit of detection of 0.12 μM applying the accumulation time of 300 s in unstirred solution. The newly developed method was successfully utilized for the direct determination of <em>BB BN</em> in spiked tap water, spiked soft drink, and pharmaceuticals. According to the Blue Applicability Great Index metric tool, the proposed method scored 72.5 points, indicating its suitability in terms of practicality and applicability.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128119"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophoresis-enhanced paper spray mass spectrometry

IF 5.6 1区化学

Talanta Pub Date : 2025-04-09 DOI: 10.1016/j.talanta.2025.128099

Dylan T. Holden , Brison A. Shira , MyPhuong T. Le, R. Graham Cooks

{"title":"Electrophoresis-enhanced paper spray mass spectrometry","authors":"Dylan T. Holden , Brison A. Shira , MyPhuong T. Le, R. Graham Cooks","doi":"10.1016/j.talanta.2025.128099","DOIUrl":"10.1016/j.talanta.2025.128099","url":null,"abstract":"<div><div>The development of ambient ionization methods, such as paper spray (PS), has streamlined the transition from a collection of molecules in a condensed-phase sample to the gas-phase ions represented in a mass spectrum. In many cases, time and resources are saved by omitting chromatography and sample preparation; yet this strategy also eliminates the opportunity to process mixtures in the condensed phase. We here report a PS method that, without additional hardware or expertise, enhances mass spectrometry (MS) analysis of mixtures through electrophoresis. By loading analyte solution onto pre-wetted paper and applying spray potential, analytes were spatiotemporally processed according to their electrophoretic mobility before being subjected to MS. The fundamental and analytical aspects of this electrophoresis-enhanced PS (EePS) were probed with polarity switching, pH changes, complex environmental matrices, and a variety of analytes, including natural products obtained directly from plant material, without chromatography. EePS provides only modest separations but exhibits wide pH tolerance, high compatibility with complex environmental matrices, excellent robustness, superb cost-effectiveness, and is amenable to novel mass analysis strategies using portable mass spectrometers <em>in situ</em>.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128099"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0