Hanna Lis, Klaudia Kwidzińska, Katarzyna Bethke, Magda Caban
{"title":"Cyanobacteria as generators of reactive oxygen species – A review of analysis methods and case studies","authors":"Hanna Lis, Klaudia Kwidzińska, Katarzyna Bethke, Magda Caban","doi":"10.1016/j.talanta.2025.128648","DOIUrl":null,"url":null,"abstract":"<div><div>Reactive oxygen species (ROS) are omnipresent in the environment and serve as important modulators of geochemical cycles. Phytoplankton is the major producer of ROS in water. Cyanobacteria, on one hand, are the most sensitive component of phytoplankton to ROS; on the other, they are producers of ROS with ecological roles that are still not fully understood. The correlation of blooms with increased ROS levels necessitates a deep understanding of the mechanisms responsible for ROS production by cyanobacteria. Reliable analytical methods are thus needed. This review begins with the presentation of ROS as a factor in water biochemistry and the interactions between ROS and cyanobacteria. It then presents reports on ROS analysis following cyanobacteria exposure to physical and chemical stressors using fluorescence probes. The possibilities for selective and qualitative analysis of ROS were critically discussed. In the conclusion section, the main drawbacks of the methods based on fluoresceine-based fluorescence probes, challenges to overcome and future needs are highlighted.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128648"},"PeriodicalIF":6.1000,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039914025011397","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Reactive oxygen species (ROS) are omnipresent in the environment and serve as important modulators of geochemical cycles. Phytoplankton is the major producer of ROS in water. Cyanobacteria, on one hand, are the most sensitive component of phytoplankton to ROS; on the other, they are producers of ROS with ecological roles that are still not fully understood. The correlation of blooms with increased ROS levels necessitates a deep understanding of the mechanisms responsible for ROS production by cyanobacteria. Reliable analytical methods are thus needed. This review begins with the presentation of ROS as a factor in water biochemistry and the interactions between ROS and cyanobacteria. It then presents reports on ROS analysis following cyanobacteria exposure to physical and chemical stressors using fluorescence probes. The possibilities for selective and qualitative analysis of ROS were critically discussed. In the conclusion section, the main drawbacks of the methods based on fluoresceine-based fluorescence probes, challenges to overcome and future needs are highlighted.
期刊介绍:
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.