Cyanobacteria as generators of reactive oxygen species – A review of analysis methods and case studies

IF 6.1 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Hanna Lis, Klaudia Kwidzińska, Katarzyna Bethke, Magda Caban
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引用次数: 0

Abstract

Reactive oxygen species (ROS) are omnipresent in the environment and serve as important modulators of geochemical cycles. Phytoplankton is the major producer of ROS in water. Cyanobacteria, on one hand, are the most sensitive component of phytoplankton to ROS; on the other, they are producers of ROS with ecological roles that are still not fully understood. The correlation of blooms with increased ROS levels necessitates a deep understanding of the mechanisms responsible for ROS production by cyanobacteria. Reliable analytical methods are thus needed. This review begins with the presentation of ROS as a factor in water biochemistry and the interactions between ROS and cyanobacteria. It then presents reports on ROS analysis following cyanobacteria exposure to physical and chemical stressors using fluorescence probes. The possibilities for selective and qualitative analysis of ROS were critically discussed. In the conclusion section, the main drawbacks of the methods based on fluoresceine-based fluorescence probes, challenges to overcome and future needs are highlighted.

Abstract Image

作为活性氧产生菌的蓝藻-分析方法和案例研究综述
活性氧(ROS)在环境中无处不在,是地球化学循环的重要调节剂。浮游植物是水中活性氧的主要产生者。一方面,蓝藻是浮游植物对活性氧最敏感的成分;另一方面,它们是ROS的生产者,其生态作用尚未完全了解。藻华与活性氧水平增加的相关性需要深入了解蓝藻产生活性氧的机制。因此需要可靠的分析方法。这篇综述首先介绍了活性氧作为水生化的一个因素以及活性氧与蓝藻之间的相互作用。然后介绍了使用荧光探针对蓝藻暴露于物理和化学压力源后的ROS分析报告。选择性和定性分析活性氧的可能性进行了批判性的讨论。在结论部分,重点介绍了基于荧光素的荧光探针的方法的主要缺点、需要克服的挑战和未来的需求。
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来源期刊
Talanta
Talanta 化学-分析化学
CiteScore
12.30
自引率
4.90%
发文量
861
审稿时长
29 days
期刊介绍: Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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