IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128605

Radovan Tomašovský , Martina Opetová , Michaela Polačková , Katarína Maráková

{"title":"Analysis of lysozyme in human saliva by CE-UV: A new simple, fast, and reliable method","authors":"Radovan Tomašovský , Martina Opetová , Michaela Polačková , Katarína Maráková","doi":"10.1016/j.talanta.2025.128605","DOIUrl":"10.1016/j.talanta.2025.128605","url":null,"abstract":"<div><div>Many studies have shown a correlation between salivary lysozyme levels and the presence of various diseases or stress. Here, we developed a new, simple method for the analysis of lysozyme in human saliva by capillary electrophoresis (CE) and UV detection that has potential application in clinical settings. The optimized method used 1 M formic acid +10 % (v/v) isopropyl alcohol as a background electrolyte, transient isotachophoresis as a preconcentration method, deactivation of the glass insert surface by carbonic anhydrase as a blocking agent to eliminate non-specific protein adsorption, and only simple dilution as a sample pretreatment. The validation showed high linearity (r<sup>2</sup> = 0.9997), inter- and intra-day accuracy (−2.03 to +5.36 %) and precision (<5 % RSD), and limit of detection (0.5 μg/mL). The stability of lysozyme in human saliva stored at −80 °C for 7 days using protease inhibitors and the variations of lysozyme excretion during the day and over 3 consecutive days were also investigated. The optimized and validated method was applied for the determination of salivary lysozyme levels in 25 healthy volunteers and the results showed the mean salivary lysozyme concentration at 14.3 ± 10.5 μg/mL. To the best of our knowledge, this is the first CE-UV method for human salivary lysozyme determination. This method meets the criteria of simplicity (minimum steps in sample pretreatment and analysis), speed (the entire process from saliva collection to result takes less than 45 min), reliability (highly satisfactory validation data), and suitability for application to real-world samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128605"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of CD81 in mouse serum-derived extracellular vesicles via inductively coupled plasma – mass spectrometry using gold-based labels 采用金基标签的电感耦合等离子体质谱法定量小鼠血清来源的细胞外囊泡中的CD81

IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128609

Jaime Martínez-García, Beatriz Fernández, Rosario Pereiro

{"title":"Quantification of CD81 in mouse serum-derived extracellular vesicles via inductively coupled plasma – mass spectrometry using gold-based labels","authors":"Jaime Martínez-García, Beatriz Fernández, Rosario Pereiro","doi":"10.1016/j.talanta.2025.128609","DOIUrl":"10.1016/j.talanta.2025.128609","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) released by cells are one of the main mechanisms of intercellular communication. Nowadays it is known that they can play important roles in diseases such as cancer or neurodegenerative processes. Therefore, it is of high interest to develop proper procedures for analyzing EVs in order to exploit their potential to understand biological dysregulations and identifying biomarkers, such as proteins. However, the determination of specific proteins in EVs typically require highly sensitive methods, particularly when working with limited sample volumes, such as mouse serum. The combined use of metal labelled immunoprobes and detection by inductively coupled plasma – mass spectrometry (ICP-MS) has proved to be an advantageous strategy for the analysis of proteins in biological samples. In this work, a methodology based on an immunoassay using antibodies labelled with gold nanoclusters, to achieve a high signal amplification, and ICP-MS detection was developed to determine target proteins in EVs from mouse blood serum. As a proof of concept, CD81, a generic marker of EVs, was investigated, and a limit of detection as low as 56 fM was obtained. CD81 levels in EVs isolated from aliquots of 0.5 mL of commercial mouse blood serum were determined after applying an optimized differential centrifugation protocol. Results obtained with the developed analytical procedure were in good agreement with those achieved with a commercial ELISA kit.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128609"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low temperature triethylamine sensing based on annealed amorphous cobalt tin oxide nanoboxes with tunable oxygen vacancies 基于可调氧空位退火无定形氧化钴锡纳米盒的低温三乙胺传感

IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128603

Jian Fang , Wei Wang , Yizhuo Fan , Dali Liu , Yu Chen , Shengping Ruan

{"title":"Low temperature triethylamine sensing based on annealed amorphous cobalt tin oxide nanoboxes with tunable oxygen vacancies","authors":"Jian Fang , Wei Wang , Yizhuo Fan , Dali Liu , Yu Chen , Shengping Ruan","doi":"10.1016/j.talanta.2025.128603","DOIUrl":"10.1016/j.talanta.2025.128603","url":null,"abstract":"<div><div>The development of triethylamine (TEA) sensors with high sensitivity and selectivity is of great significance for realizing multi-field applications such as environmental monitoring and industrial safety. In this study, a novel and high-performance TEA sensor strategy based on single-component amorphous materials is proposed. By employing CoSn(OH)<sub>6</sub> nanocubes as precursors, amorphous hollow CoSnO<sub>3</sub> nanoboxes were successfully synthesized via an alkali etching process followed by a controlled annealing procedure. The results showed that annealing temperature accurately regulated the degree of amorphous and oxygen vacancy concentration of the material. Based on the analysis of characterization test results, it was confirmed that the unique hollow nanobox structure formed through alkali etching significantly enhanced the specific surface area of the pristine nanocube, reaching up to 262.4 m<sup>2</sup>/g. Gas sensitive tests demonstrated that the response value (Rg/Ra = 24.5) of the HCSO-200 sensor to 50 ppm TEA at low operating temperatures (120<sup>o</sup>C) was nearly 4.2 times higher than that of crystalline material, with excellent repeatability and long-term stability. Further mechanistic studies showed that the excellent TEA sensing performance of the HCSO-200 material could be attributed to its amorphous structure, hollow morphology, and optimized oxygen vacancy concentration by controlling annealing temperature. This work not only provides an efficient one-component sensing material for TEA detection, but also broadens the idea of other amorphous semiconductor materials for gas detection.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128603"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gel-free capillary zone electrophoresis at acidic pH for micro DNA and RNA analysis 酸性无凝胶毛细管区带电泳用于微DNA和RNA分析

IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128610

Jakob Haglöf , Erik Bivehed , Cari Sänger-van de Griend

引用次数: 0

The application of desorption electrospray ionization mass spectrometry and mass spectrometry imaging in metabolomics, lipidomics and proteomics analysis 解吸电喷雾质谱和质谱成像在代谢组学、脂质组学和蛋白质组学分析中的应用

IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128611

Haofan Liu , Sicheng Huang , Lina Yang , Yaqian He , Yongshuai Jing , Yinghua Xie , Beibei Hu , Zhongqiu Li , Haichao Bi , Zhiwei Li

{"title":"The application of desorption electrospray ionization mass spectrometry and mass spectrometry imaging in metabolomics, lipidomics and proteomics analysis","authors":"Haofan Liu , Sicheng Huang , Lina Yang , Yaqian He , Yongshuai Jing , Yinghua Xie , Beibei Hu , Zhongqiu Li , Haichao Bi , Zhiwei Li","doi":"10.1016/j.talanta.2025.128611","DOIUrl":"10.1016/j.talanta.2025.128611","url":null,"abstract":"<div><div>At present, mass spectrometry (MS) and mass spectrometry imaging (MSI) have developed into versatile analytical techniques, applicable for the analysis of both small molecules and macromolecular compounds and complexes. Desorption electrospray ionization (DESI), as an ambient MS (AMS) technique, enables rapid analysis and imaging under open conditions with minimal or no sample preparation required. In this review, the principles and mechanisms of DESI were described, especially for the factors affecting the analysis including suppression effects, device condition parameter settings, and ionization and transmission efficiency. Moreover, the relevant improvements of DESI technique were introduced in detail. Emphasis has been made to discuss the recent research on the application of DESI MS and MSI in the fields of metabolomics, lipidomics, and proteomics. In these applications, DESI MS and MSI exhibit exceptional capabilities for qualitative analysis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128611"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic chip coupled with photoelectrochemical/fluorescence dual-modal sensing system for the efficient enrichment and detection of circulating tumor cells 微流控芯片耦合光电化学/荧光双模态传感系统用于循环肿瘤细胞的高效富集和检测

IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128613

Qingfeng Lin , Xiaoyang Chen , Yawen Hong , Jingyu Huang , Jiuhong Li , Yanying Wang , Lei Chen , Chunya Li , Gang Luo

{"title":"Microfluidic chip coupled with photoelectrochemical/fluorescence dual-modal sensing system for the efficient enrichment and detection of circulating tumor cells","authors":"Qingfeng Lin , Xiaoyang Chen , Yawen Hong , Jingyu Huang , Jiuhong Li , Yanying Wang , Lei Chen , Chunya Li , Gang Luo","doi":"10.1016/j.talanta.2025.128613","DOIUrl":"10.1016/j.talanta.2025.128613","url":null,"abstract":"<div><div>Conventional percutaneous lung biopsy and imaging modalities are often associated with adverse effects and may yield false-negative results, limiting their clinical efficacy. Circulating tumor cells (CTCs) are pivotal mediators in early tumorigenesis and metastatic dissemination. The detection of CTCs offers unparalleled advantages for early diagnosis and metastasis monitoring. NCI–H460 and NCI–H1650 cell lines serve as representative models for non-small cell lung cancer (NSCLC) and are critical biomarkers given that NSCLC accounts for over 85 % of lung cancer cases. Effective enrichment and precise identification of CTCs from whole blood are essential for early diagnostic applications. Herein, a microfluidic platform integrated with a dual-modal detection system, comprising photoelectrochemical (PEC) sensing and fluorescence imaging, was fabricated for the enrichment and quantification of CTCs (NCI–H460 and NCI–H1650 cells). The Hcy-Thiol probe-labeled CTCs in whole blood were separated by a microfluidic chip, and then a cathodic photoelectrochemical sensing system based on different aptamer-modified CuInS<sub>2</sub> nanoflowers was used to selectively capture CTCs and provide photocurrent signals, thus achieving fluorescence/photoelectrochemical dual-mode detection. Under the optimized conditions, the separation efficiency and purity of CTCs were 83.4 % and 76.8 %, respectively. Quantitative analysis of spiked blood samples at 100 cells/mL yielded cell counts of 107 ± 5 NCI–H460 cells and 87 ± 4 NCI–H1650 cells via photocurrent responses, corroborated by fluorescence signals with counts of 110 ± 4 and 85 ± 3 cells/mL, respectively. This microfluidic integrated with PEC/fluorescence dual-modal sensing system exhibits promising potential for early NSCLC detection and metastasis evaluation with high specificity and sensitivity.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128613"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an SI-traceable magnetic solid-phase extraction coupled with isotope dilution LC-MS/MS method for accurate quantification of alpha-fetoprotein in human serum si可追溯磁固相萃取-同位素稀释LC-MS/MS法测定人血清中甲胎蛋白含量

IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128606

Haofeng Sun , Jianyi Liu , Qi Zhang , Lei Yang , Dan Song , Min Zhou , Dewei Song

{"title":"Development of an SI-traceable magnetic solid-phase extraction coupled with isotope dilution LC-MS/MS method for accurate quantification of alpha-fetoprotein in human serum","authors":"Haofeng Sun , Jianyi Liu , Qi Zhang , Lei Yang , Dan Song , Min Zhou , Dewei Song","doi":"10.1016/j.talanta.2025.128606","DOIUrl":"10.1016/j.talanta.2025.128606","url":null,"abstract":"<div><div>Alpha-fetoprotein (AFP) is a critical biomarker widely used for the screening and diagnosis of hepatocellular carcinoma and germ cell tumors. Although immunoassays are commonly employed for AFP detection, significant discrepancies in measurement results across different laboratories persist due to poor harmonization among methods. To improve the accuracy and harmonization of AFP quantification, this study developed a novel magnetic solid-phase extraction coupled with isotope dilution liquid chromatography-tandem mass spectrometry (MSPE-ID-LC-MS/MS) method for the precise measurement of AFP in serum. The method utilizes high-affinity AFP antibodies cocktails conjugated with magnetic nanoparticles to enhance the recovery of low-abundance AFP, combined with optimized elution conditions to ensure analytical reliability. The protocol involves one-step denaturation and alkylation, followed by tryptic digestion. This enables accurate quantification with SI-traceability using three signature peptides. Method validation demonstrated that intra- and inter-day precisions were <10 %, recovery rates of 99.8–101.4 %, and the limit of quantification (LOQ) of 1.5 ng/mL. This work establishes a metrologically robust reference procedure for AFP measurement and paves the way for standardization of clinical protein assays.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128606"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative lipidomics for three-dimensional cell culture using deuterium oxide metabolic labeling 使用氧化氘代谢标记的三维细胞培养定量脂质组学

IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128612

Jonghyun Kim , Kyoung-Jin Choi , Sung Bum Park , Yoon-Ju Na , Ki Young Kim , Tae-Young Kim

{"title":"Quantitative lipidomics for three-dimensional cell culture using deuterium oxide metabolic labeling","authors":"Jonghyun Kim , Kyoung-Jin Choi , Sung Bum Park , Yoon-Ju Na , Ki Young Kim , Tae-Young Kim","doi":"10.1016/j.talanta.2025.128612","DOIUrl":"10.1016/j.talanta.2025.128612","url":null,"abstract":"<div><div>Three-dimensional (3D) cell culture offers a more physiologically relevant model than traditional two-dimensional culture, yet standardized methods for lipid quantification in 3D systems are lacking. This study presents a novel quantitative lipidomic approach combining 3D culture with deuterium oxide (D<sub>2</sub>O) metabolic labeling to provide comprehensive insights into metabolic alterations. Using a hydrogel-based 3D system, we cultured preadipocytes and adipocytes, incorporating macrophage co-culture to induce insulin resistance. Relative lipid quantification was achieved using D<sub>2</sub>O labeling for global omics relative quantification (DOLGOReQ). This method enabled the quantification of hundreds of lipids across major categories, including glycerolipids, glycerophospholipids, fatty acyls, and sphingolipids, while also revealing cell-type-specific D-labeling efficiencies. DOLGOReQ analysis revealed that macrophage co-culture significantly reduced long-chain free fatty acids and triacylglycerols (TGs). Quantitative correlation analysis between TGs and free fatty acids indicated that the macrophage-mediated TG reduction stemmed from decreased free fatty acid availability, the precursors for lipid synthesis. Furthermore, macrophages increased D-labeling efficiency, suggesting enhanced lipolysis contributing to TG reduction. DOLGOReQ not only facilitates relative quantification of lipid changes but also provides valuable insights into lipid turnover dynamics. These findings establish DOLGOReQ as a powerful tool for investigating global lipid metabolism changes induced by external stimuli in 3D cell culture.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128612"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural-derived sorbents: Application of biochar materials as green extractive approach in food analysis 天然吸附剂：生物炭材料作为绿色萃取方法在食品分析中的应用

IF 5.6 1区化学

Talanta Pub Date : 2025-07-21 DOI: 10.1016/j.talanta.2025.128608

Carla Iglesias-Martín, Ana M. Ares, José Bernal, Adrián Fuente-Ballesteros

{"title":"Natural-derived sorbents: Application of biochar materials as green extractive approach in food analysis","authors":"Carla Iglesias-Martín, Ana M. Ares, José Bernal, Adrián Fuente-Ballesteros","doi":"10.1016/j.talanta.2025.128608","DOIUrl":"10.1016/j.talanta.2025.128608","url":null,"abstract":"<div><div>The transition toward greener methodologies in analytical chemistry has intensified interest in biochar as a sustainable sorbent for food analysis. Derived from the pyrolysis of agro-industrial residues, biochar combines low-cost production with good properties such as high surface area, porosity, and surface tunability. This review provides a critical and feedstock-oriented overview of biochar applications in food sample preparation, categorizing sorbents based on their biomass origin (fruit waste, nut and seed residues, cereal by-products, lignocellulosic fibers, and wood waste). Each source is examined in terms of its physicochemical characteristics, extraction efficiency, and performance across different food matrices. Special emphasis is placed on sorbent modification strategies, the use of environmentally compatible desorption solvents, and the alignment of biochar use with green analytical chemistry (GAC) principles. Additionally, the review identifies key research gaps, limitations in analytical reproducibility, and challenges for regulatory acceptance. Overall, biochar emerges as a versatile and eco-efficient material with strong potential to enhance sustainability in food safety analysis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128608"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymmetrically modified hydrogel nanochannel biosensor for label-free and selective kanamycin monitoring 用于无标记和选择性卡那霉素监测的非对称修饰水凝胶纳米通道生物传感器

IF 5.6 1区化学

Talanta Pub Date : 2025-07-19 DOI: 10.1016/j.talanta.2025.128590

Lili Liu, Xingtong Liu, Linna Li, Yandi Hui, Jiajia Lu, Zhen Li, Fujun Yao, Xiaofeng Kang, YanLi Guo

{"title":"Asymmetrically modified hydrogel nanochannel biosensor for label-free and selective kanamycin monitoring","authors":"Lili Liu, Xingtong Liu, Linna Li, Yandi Hui, Jiajia Lu, Zhen Li, Fujun Yao, Xiaofeng Kang, YanLi Guo","doi":"10.1016/j.talanta.2025.128590","DOIUrl":"10.1016/j.talanta.2025.128590","url":null,"abstract":"<div><div>Nanochannel sensors have attracted much attention in the field of biosensing due to their unique domain-limiting effect and signal transduction mechanism. However, the traditional surface modification strategy often suffers from the low probe immobilisation efficiency, channel clogging and limited modification sites. To address these challenges, this study proposes a synergistic construction strategy combining hydrogel filling and asymmetric modification. Using polyethylene terephthalate (PET) membranes as the substrate, we constructed acrylic acid (AAc)-co-acrylamide (AAm)-co-methyl methacrylate (MMA) ternary copolymerised hydrogel within cylindrical nanochannel through free radical polymerisation reaction. Further functionalization was achieved through PEI/Zr<sup>4+</sup> asymmetric modification, enabling precise charge gradient regulation and yielding a composite nanochannel with significant ionic current rectification (ICR) effect. As a proof of concept, kanamycin was detected with high sensitivity through aptamer recognition, triggered DNA conformational transitions, and specific coordination of Zr<sup>4+</sup> to double-stranded DNA (dsDNA). The hydrogel's three-dimensional porous architecture significantly improves the probe loading capacity while the charge gradient optimization and the signal amplification mechanism collectively enables the sensing platform to exhibit excellent analytical performance and stability. It provides a new idea for the development of high-performance biosensors with important applications in the fields of food safety and clinical diagnosis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128590"},"PeriodicalIF":5.6,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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