IF 5.6 1区化学

Talanta Pub Date : 2025-02-09 DOI: 10.1016/j.talanta.2025.127705

Jiwei Li, Guocai Liu, Haixiang Yu, Hongyue Ma, Xiufeng Liu, Jiangwei Tian, Boyang Yu

{"title":"Generation of cross-reactive DNA aptamers to construct the fluorescent sensing array for identifying the origin of Toad Venom.","authors":"Jiwei Li, Guocai Liu, Haixiang Yu, Hongyue Ma, Xiufeng Liu, Jiangwei Tian, Boyang Yu","doi":"10.1016/j.talanta.2025.127705","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.127705","url":null,"abstract":"Toad venom, a traditional Chinese medicine, has long been used to treat various challenging ailments. Its effectiveness and toxicity can vary depending on the types and concentrations of bufadienolides, which vary from region to region. However, identifying the origin of toad venom is challenging due to the absence of distinct visual characteristics of the original animals. Therefore, developing a scientific and practical method for origin identification is crucial to ensure the safety and efficacy of toad venom. Integrating a fluorescent sensing array with cross-reactive aptamers provides a promising solution to this issue. We isolated cross-reactive aptamers using a combination of complex target-directed SELEX and convergent selection strategies. During the selection process, we used an immobilized stem-loop library to select aptamers and evaluated the enrichment rate and pool affinity using gel elution assays. After high-throughput sequencing, we selected three cross-reactive aptamers designated N4.8, N2.4, and S1 that exhibit distinct binding profiles for bufadienolides as the biorecognition elements for the fluorescent sensing array. This sensor is capable of distinguishing toad venom from different origins with high accuracy (98.7 %), offering convenient operation, and providing a new method for detecting the origin of toad venom.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127705"},"PeriodicalIF":5.6,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of a porous zirconium-based MOF nanoplate as an affinity ECL platform for the detection of protein kinase activity and inhibitor screening.

IF 5.6 1区化学

Talanta Pub Date : 2025-02-08 DOI: 10.1016/j.talanta.2025.127675

Sasan Radfar, Milad Sheikh, Amirreza Akhavantabib, Amirhossein Heidari, Milad Ghasemi, Mohammadreza Naghavi, Reza Ghanbari, Farkhonde Zibadi, Babak Jamshidi, Abdolhamid Alizadeh

{"title":"Application of a porous zirconium-based MOF nanoplate as an affinity ECL platform for the detection of protein kinase activity and inhibitor screening.","authors":"Sasan Radfar, Milad Sheikh, Amirreza Akhavantabib, Amirhossein Heidari, Milad Ghasemi, Mohammadreza Naghavi, Reza Ghanbari, Farkhonde Zibadi, Babak Jamshidi, Abdolhamid Alizadeh","doi":"10.1016/j.talanta.2025.127675","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.127675","url":null,"abstract":"Abnormal kinase expression affects phosphorylation in the human body, which is associated with numerous diseases, including cancer, diabetes mellitus, and Alzheimer's disease. In this study, we synthesized a highly stable, two-dimensional, luminescence-functionalized metal-organic framework with remarkable electrochemiluminescence (ECL) by immobilizing 9,10-Di(p-carboxyphenyl) anthracene (dca) on a zirconium cluster (dca-Zr₁₂) via a strong coordination bond between -COO⁻ and Zr⁴⁺. This novel and simple platform relies on the highly specific identification of phosphate molecules by the ultra-thin dca-Zr₁₂ nanoplate through carboxylate-Zr⁴⁺-phosphate chemistry. The ferrocene-labeled peptide substrate (Fc-S-Peptide) was phosphorylated in the presence of protein kinase A (PKA) and adenosine 5'-triphosphate (ATP), and the resulting phosphopeptide could subsequently be precisely captured by the zirconium sites of the dca-Zr12-modified electrode and, eventually, quench the ECL and gain a signal-off state. This rapid and simple detection strategy was successfully employed to measure PKA activity, with a detection limit as low as 0.35 mU mL-1. Based on the results, it exhibited high selectivity and can be applied for screening PKA inhibitors. The technique was subsequently applied to detect protein kinase activity in drug-stimulated MCF-7 cell lysates, demonstrating its potential for kinase-related investigations. Further, this platform could identify the activity of other kinase types with universal applicability.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127675"},"PeriodicalIF":5.6,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyacrylamide-incorporated copper electrodes for electrochemical-colorimetric dual-mode detection of H₂O₂ released from tomato leaves.

IF 5.6 1区化学

Talanta Pub Date : 2025-02-08 DOI: 10.1016/j.talanta.2025.127689

Linyan Su, Yuan Li, Huiyuan Chen, Xiao Liu, Zhiyao Zhang, Duan Bin, Beibei Yang, Lijun Sun, Hongbin Lu, Baohong Liu

{"title":"Polyacrylamide-incorporated copper electrodes for electrochemical-colorimetric dual-mode detection of H2O2 released from tomato leaves.","authors":"Linyan Su, Yuan Li, Huiyuan Chen, Xiao Liu, Zhiyao Zhang, Duan Bin, Beibei Yang, Lijun Sun, Hongbin Lu, Baohong Liu","doi":"10.1016/j.talanta.2025.127689","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.127689","url":null,"abstract":"Hydrogen peroxide (H2O2) plays a key role in a diverse array of cellular signaling pathways, which is closely related to plant health and physiological status. The accurate and efficient monitoring of H2O2 in living plant cells has attracted enormous interest. Herein, we developed an electrochemical-colorimetric dual-mode sensor based on the peroxidase-like activity of a polyacrylamide (PAM) -modified copper electrode (Cu-PAM), allowing for the in situ detection of H2O2 released from tomato leaves. The co-electrodeposition of unique combination of Cu and polyacrylamide, as well as the active site structure of Cu-centered peroxidases, can enhance adsorption performance by the hydrogen bonds between PAM with H2O2, which exhibited excellent electrochemical performance with a low limit of detection (LOD) of 0.0167 mM and a detection range of 0.05-25.31 mM. Meanwhile, a colorimetric signal output of the sensor that can be quantified from 1 μM to 70 μM with a LOD value of 0.33 μM. This work demonstrates a huge potential application prospect of the polyacrylamide-modified copper in the field of biosensors.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127689"},"PeriodicalIF":5.6,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ultrasensitive homogeneous electrochemical strategy for ochratoxin a sensing based on nanoscale PCN-224@MB@Apt.

IF 5.6 1区化学

Talanta Pub Date : 2025-02-08 DOI: 10.1016/j.talanta.2025.127695

Xi Wu, Chenggang Geng, Weiwei Cheng, Zhenjiong Wang, Yan Zhang, Di Wu, Xiaozhi Tang

{"title":"An ultrasensitive homogeneous electrochemical strategy for ochratoxin a sensing based on nanoscale PCN-224@MB@Apt.","authors":"Xi Wu, Chenggang Geng, Weiwei Cheng, Zhenjiong Wang, Yan Zhang, Di Wu, Xiaozhi Tang","doi":"10.1016/j.talanta.2025.127695","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.127695","url":null,"abstract":"In this reasearch, a homogeneous electrochemical sensor based on PCN-224@MB@Apt was fabricated for the ultrasensitive determination of ochratoxin A (OTA). Firstly, nanoscale PCN-224 were synthesized as the nanocarrier to embed the signal probe of methylene blue (MB). Then, the OTA aptamer (Apt) was added and connected to PCN-224@MB via the Zr-O-P bond between Zr metal sites of PCN-224 and phosphate group of Apt as the biogate. When OTA exists, the Apt would preferentially bind with OTA and fall off from PCN-224@MB, leading to the release of MB and generation of differential pulse voltammetry (DPV) response. The DPV response of MB was linearly correlated with the amount of OTA. The optimized PCN-224@MB@Apt sensor showed outstanding detection performance towards OTA with a low detection limit of 2.6 × 10-5 ng/mL (S/N = 3) and wide linear range (10-4-10 ng/mL). Meanwhile, the fabricated homogeneous electrochemical sensor exhibited splendid stability, reproducibility, and specificity. To assess the practical applicability, the PCN-224@MB@Apt sensor was applied to detect OTA in real corn samples and desirable recovery rates varying from 83.2 % to 109.6 % were obtained.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127695"},"PeriodicalIF":5.6,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A ratio luminescent europium organometallic gel for in vitro detection of hepatocellular carcinoma marker and cellular imaging

IF 5.6 1区化学

Talanta Pub Date : 2025-02-07 DOI: 10.1016/j.talanta.2025.127708

Xiao Lian , Fang Chen , Yanmin Zhang , Juzhou Zhang , Bangben Yao , Helin Niu

{"title":"A ratio luminescent europium organometallic gel for in vitro detection of hepatocellular carcinoma marker and cellular imaging","authors":"Xiao Lian , Fang Chen , Yanmin Zhang , Juzhou Zhang , Bangben Yao , Helin Niu","doi":"10.1016/j.talanta.2025.127708","DOIUrl":"10.1016/j.talanta.2025.127708","url":null,"abstract":"<div><div>Hepatocellular carcinoma is a particularly aggressive form of liver malignancy, representing one of the most formidable threats to human health and a major contributor to cancer-related deaths. Cholyglycine (CG), which plays a pivotal role in lipid metabolism, has garnered attention as a potential biomarker for hepatocellular carcinoma. Nevertheless, the structural resemblance of CG to various bile acids complicates the specific identification of CG. Therefore, the effective monitoring of CG in biological samples is still a challenge to be solved. In this study, a europium-based metal organic gel (Eu-MOG) with dual emission was synthesized and displayed an obvious luminescent color change from red to blue for CG. The synthesized Eu-MOG exhibits excellent selectivity towards CG, and enables the sensitive detection of CG in serum with LOD as 307 ppb. This character of Eu-MOG has also been validated in cell imaging for CG, which make this europium-based probe sufficient for clinical monitoring of CG to diagnosis hepatocellular carcinoma. The results of our experiments, corroborated by theoretical calculations, indicate that the high sensitivity of MOG to CG stems from the intermolecular N–H⋯O interaction between CG and the ligand H2NDC. This interaction facilitates intermolecular charge transfer, which in turn alters the luminescence of the europium-based metal-organic gel (Eu-MOG). This study provides a robust platform for the early diagnosis of hepatocellular carcinoma and contributes significantly to the evaluation of human hepatobiliary metabolic status.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127708"},"PeriodicalIF":5.6,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cascade-amplification-based electrochemical detection of Akashiwo sanguinea at pre-outbreak stage.

IF 5.6 1区化学

Talanta Pub Date : 2025-02-06 DOI: 10.1016/j.talanta.2025.127671

Changrui Ye, Hongjie Liu, Shaopeng Wang, Man Zhang, Chaoxin Zhang, Fulin Yang, Fang Shen, Liwei Wang

{"title":"Cascade-amplification-based electrochemical detection of Akashiwo sanguinea at pre-outbreak stage.","authors":"Changrui Ye, Hongjie Liu, Shaopeng Wang, Man Zhang, Chaoxin Zhang, Fulin Yang, Fang Shen, Liwei Wang","doi":"10.1016/j.talanta.2025.127671","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.127671","url":null,"abstract":"Red tide events caused by Akashiwo sanguinea (A. sanguinea) pose a significant threat to ecosystems. However, studies that offer promising approaches for portable and onsite detection with precise identification of A. sanguinea remain insufficient. In this study, we developed an electrochemical biosensor (E-biosensor) for detecting A. sanguinea combined with cascade amplification strategies, termed TDW-E-biosensor. A predictive relationship was also established to predict algal cell density based on electrochemical signals. The experiment results showed that the TDW-E-biosensor was successfully applied for detecting A. sanguinea at the pre-outbreak stage and demonstrated excellent analytical performance, showing a low limit of detection (LOD) of 0.0676 fM and quantitation (LOQ) of 0.102 fM for the three-electrode system, and a low LOD of 6.873 fg μL-1 and LOQ of 20.460 fg μL-1 for the portable system. The accuracy of the TDW-E-biosensor was validated through comparison with droplet digital PCR (ddPCR) and Bland-Altman analysis, demonstrating a high level of agreement (a mean difference of 0.132 and a standard deviation of 0.184). The reliability of the predictive relationship was evidenced by controlled laboratory experiments and Bland-Altman analysis. The developed TDW-E-biosensor provides an innovative and promising tool for early warning efforts regarding harmful algae.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127671"},"PeriodicalIF":5.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple analytical method to determine urinary isotopic enrichment of phenylalanine by GC/EI-MS coupled with pentafluorobenzyl derivatization. 利用 GC/EI-MS 结合五氟苄基衍生法测定尿液中苯丙氨酸同位素富集的简便分析方法。

IF 5.6 1区化学

Talanta Pub Date : 2025-02-06 DOI: 10.1016/j.talanta.2025.127697

Hidetoshi Kumata, Haruka Ochiai, Anna Tanaka, Shiori Oda, Kazuko Ishikawa-Takata, Tomonari Umemura

{"title":"Simple analytical method to determine urinary isotopic enrichment of phenylalanine by GC/EI-MS coupled with pentafluorobenzyl derivatization.","authors":"Hidetoshi Kumata, Haruka Ochiai, Anna Tanaka, Shiori Oda, Kazuko Ishikawa-Takata, Tomonari Umemura","doi":"10.1016/j.talanta.2025.127697","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.127697","url":null,"abstract":"The indicator amino acid oxidation (IAAO) technique estimates the physiological requirements for amino acids and proteins in living organisms, including humans. It involves monitoring urinary amino acids and exhaled CO2 after ingesting 1-13C-labeled (carboxy-labeled) amino acids. The most common IAAO indicator amino acid is 1-13C-labeled phenylalanine ([1-13C]Phe). Its urinary concentration in test subjects ranges from below the detection limit to several μM. A simple analytical method for distinguishing trace amounts of [1-13C]Phe in urine from high levels of naturally occurring Phe is crucial for making IAAO tests easier. This study presents a simple and reliable approach for the simultaneous quantification of [1-13C]Phe and Phe in human urine using conventional GC-EI-MS. In this method, urinary phenylalanine is reacted with pentafluorobenzyl bromide in a single-phase solvent system of acetone-borate buffer without dehydration or desalting to form disubstituted pentafluorobenzyl (PFB) derivatives, which are then analyzed by GC-EI-MS (SIM). The Phe and [1-13C]Phe PFB derivative peaks eluted at the same retention time on the gas chromatogram but could be differentiated on the basis of fragment ions (m/z 434, 435) derived from the loss of the phenyl group ([M - 91]+). Correcting the interference of the m+1 isotope peak of Phe in the [M - 91] fragment (m/z 435) of [1-13C]Phe using the m/z 434 peak intensity and natural isotope ratio, both Phe and [1-13C]Phe could be quantified in the concentration range found in urine. The method was successfully applied to examine the temporal enrichment of [1-13C]Phe in urine samples obtained from IAAO subjects following the ingestion of a test meal containing [1-13C]Phe.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127697"},"PeriodicalIF":5.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural deep eutectic solvent-based hollow polymer inclusion membrane doped with TiO2 nanoparticles: A new design of polymer inclusion membrane for on-chip electromembrane extraction of fluoroquinolones from food samples prior to liquid chromatography tandem mass analysis

IF 5.6 1区化学

Talanta Pub Date : 2025-02-06 DOI: 10.1016/j.talanta.2025.127696

Mahsa Shirkhodaie , Shahram Seidi , Farzaneh Shemirani , Hanieh Haghgouei

{"title":"Natural deep eutectic solvent-based hollow polymer inclusion membrane doped with TiO2 nanoparticles: A new design of polymer inclusion membrane for on-chip electromembrane extraction of fluoroquinolones from food samples prior to liquid chromatography tandem mass analysis","authors":"Mahsa Shirkhodaie , Shahram Seidi , Farzaneh Shemirani , Hanieh Haghgouei","doi":"10.1016/j.talanta.2025.127696","DOIUrl":"10.1016/j.talanta.2025.127696","url":null,"abstract":"<div><div>A new design of polymer inclusion membrane has been proposed with the aim of addressing issues with using common forms of liquid membranes. To do so, a natural deep eutectic solvent (NADES) was employed as an extractant in the structure of the hollow polymer inclusion membrane (HPIM). Besides polyvinyl chloride (PVC) as a base polymer, titanium dioxide nanoparticles (TiO2) as well as polyethylene glycol polymer (PEG) were incorporated into the HPIM structure to achieve a nanocomposite form with a desirable hydrophilicity. The optimal HPIM was composed of 12.5 v/w% of thymol-coumarin NADES, 3.0 w/w% of TiO2 and 40.0 v/w% of PEG based on PVC content. Aiming to compare the applicability, HPIMs with other types of extractants, such as bis(2-ethylhexyl) phthalate (DEHP), and the mixture of DEHP-NADES were also fabricated. To confirm the successful fabrication of the HPIM, containing the aforementioned extractant doped with TiO2 nanoparticles various characterization techniques were employed. The resultant HPIM was employed as a liquid membrane in an on-chip electromembrane extraction (EME) of fluoroquinolones (FQs) from various samples, followed by LC-MS/MS analysis. The parameters influencing extraction performance were analyzed, and the proposed method was validated under ideal conditions. All the samples provided excellent performance concerning limits of detection (0.01–0.08 ng mL−1), and quantification (0.03–0.25 ng mL−1) together with an excellent linearity (R2 ≥ 0.9978). The method indicates the desirable RSDs% in the range of 3.2–7.0 % (intra-day, n = 3 × 3) and 3.8–6.1 % (inter-day, n = 3 × 3) for three spiked levels. The satisfactory relative recoveries fell within the 92.0–115.0 % range.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127696"},"PeriodicalIF":5.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into selective behavior of NiFe2O4/graphene oxide@polyaniline nanocomposite as a novel adsorbent toward bioactive natural pigments

IF 5.6 1区化学

Talanta Pub Date : 2025-02-05 DOI: 10.1016/j.talanta.2025.127702

Azam Jabbari, Morteza Jabbari, Ehsan Nazarzadeh Zare

{"title":"Insights into selective behavior of NiFe2O4/graphene oxide@polyaniline nanocomposite as a novel adsorbent toward bioactive natural pigments","authors":"Azam Jabbari, Morteza Jabbari, Ehsan Nazarzadeh Zare","doi":"10.1016/j.talanta.2025.127702","DOIUrl":"10.1016/j.talanta.2025.127702","url":null,"abstract":"<div><div>This research focuses on the selective adsorption capability of a synthesized graphene oxide-based magnetic nanocomposite, NiFe2O4/GO@PANI, specifically engineered for the capture of a group of structurally similar flavonoid pigments from organic/aqueous solutions. Various analytical techniques (including FT-IR, TGA, VSM, Zeta potential, BET, FE-SEM and EDX) were employed to elucidate the structural details of the nanocomposite, confirming the successful preparation of NiFe2O4/GO@PANI. Zeta potential analysis revealed a zeta pH of 7.3, consistent with the results obtained from pH optimization. Furthermore, VSM analysis effectively demonstrated the magnetic properties of the nanocomposite. Additionally, TGA results confirmed the thermal stability of the nanocomposite. Key parameters affecting adsorption such as solution pH, amount of adsorbent, exposure time, pigment concentration and temperature were systematically investigated. The results indicated high adsorption capacity (qe = 16300 μg/g) and selective affinity for quercetin compared to other flavonoid pigments such as naringenin, chrysin and daidzein. Kinetic and isotherm analyses revealed that the adsorption process closely follows the nonlinear pseudo-second-order kinetic model and the Nonlinear Freundlich isotherm. Furthermore, evaluation of the temperature effect confirmed a thermodynamically favorable adsorption process. Notably, regeneration tests confirmed the stability and reusability of the NiFe₂O₄/GO@PANI nanocomposite, which are essential for practical applications in natural pigment adsorption. The efficiency of synthesized nanocomposite toward quercetin in real samples such as yellow onion, red onion and red onion peel was also evaluated. This nanocomposite demonstrated an adsorption efficiency of 85.12 % (qe = 19823.4 <math><mrow><mi>μ</mi></mrow></math> g/g) for quercetin present in the red onion peel.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127702"},"PeriodicalIF":5.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effervescence-assisted salting-out liquid-liquid extraction for rapid and convenient analysis of pyrethroid pesticide residues.

IF 5.6 1区化学

Talanta Pub Date : 2025-02-05 DOI: 10.1016/j.talanta.2025.127704

Wenxuan Li, Yaxi Xu, Meifei Wang, Yuyang Wang, Jinchao Wei, Di Chen

{"title":"Effervescence-assisted salting-out liquid-liquid extraction for rapid and convenient analysis of pyrethroid pesticide residues.","authors":"Wenxuan Li, Yaxi Xu, Meifei Wang, Yuyang Wang, Jinchao Wei, Di Chen","doi":"10.1016/j.talanta.2025.127704","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.127704","url":null,"abstract":"A simple and efficient effervescence-assisted salting-out assisted liquid-liquid extraction (EA-SALLE) method was developed for the rapid extraction of pyrethroid insecticides in fruit juices and herbal extracts. By integrating effervescence extraction with SALLE, the extraction and phase separation processes were conducted simultaneously, significantly simplifying the experimental procedure and reducing the extraction time to just 3 min. Key factors influencing the method's efficiency were systematically optimized. Combined with gas chromatography with an electron capture detector, enabled the determination of nine pyrethroid insecticides, demonstrating excellent linearity (R2 > 0.99), low limits of detection (0.03-0.17 ng/mL), satisfactory accuracy (recoveries ranging from 83.0 % to 107.9 %), and good precision (RSD < 9.1 %). The proposed EA-SALLE method offers a simple, efficient, and cost-effective sample preparation technique. It is characterized by ease of operation, short extraction time, and high sensitivity, providing an effective tool for the monitoring and assessment of pesticide residues in complex samples.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127704"},"PeriodicalIF":5.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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