IF 6.1 1区化学

Talanta Pub Date : 2025-10-05 DOI: 10.1016/j.talanta.2025.128913

Suzhen Zhang, Rongzhi Liu, Ruikang Liu, Xueqin Zhao, Hongming Fang, Yaping Zhang, Yuan Qin, Biao Huang

{"title":"Ultrasensitive miRNA-21 detection via catalytic hairpin assembly-enhanced light-initiated chemiluminescence for early cancer diagnosis.","authors":"Suzhen Zhang, Rongzhi Liu, Ruikang Liu, Xueqin Zhao, Hongming Fang, Yaping Zhang, Yuan Qin, Biao Huang","doi":"10.1016/j.talanta.2025.128913","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128913","url":null,"abstract":"MicroRNAs (miRNAs) are promising molecular markers for early-stage cancer, enabling advancements in early diagnosis, precision treatment, and prognosis evaluation. However, their detection remains challenging due to low abundance, driving the demand for highly sensitive and accurate sensing platforms. Herein, we developed a cascade amplification strategy integrating catalytic hairpin assembly (CHA) with a light-initiated chemiluminescent assay (LiCA) for ultrasensitive detection of serum miRNA-21. The platform employs H1-functionalized chemibeads (1O2-luminescent acceptors) and H2-conjugated sensibeads (photosensitizer donors), wherein target miRNA triggers ternary complex formation and initiates autonomous CHA cycling. This process facilitates femtomolar-sensitive, low-background detection without RNA extraction by utilizing proximity-driven (<200 nm) singlet oxygen transfer to generate a collective chemiluminescence signal. The assay exhibits high sensitivity (LOD: 0.21 pM) and excellent linearity (R2 = 0.9958), coupled with strong matrix tolerance in 2 % human serum, as evidenced by accurate and consistent recoveries (98.8-112 %) with <8 % deviation at spiked concentrations from 0.1 to 5 pM. When applied to clinical serum samples (n = 78), the platform demonstrated perfect diagnostic discrimination (AUC = 1.00) with 100 % sensitivity and specificity at a cutoff of 0.667 pM across breast, gastric, and colorectal cancers. A strong correlation with qPCR (r = 0.78) was observed, and miRNA-21 levels were significantly elevated in cancer patients compared to healthy controls. This platform combines the programmability of CHA with the anti-interference advantages of LiCA, offering a robust tool for low-abundance miRNA analysis in complex matrices.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt A","pages":"128913"},"PeriodicalIF":6.1,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of polymer concentration in co-flowing viscoelastic separation of submicron particles 聚合物浓度对亚微米颗粒共流粘弹性分离的影响

IF 6.1 1区化学

Talanta Pub Date : 2025-10-05 DOI: 10.1016/j.talanta.2025.128956

Samith Hettiarachchi , Dan Yuan , Nam-Trung Nguyen , Jun Zhang

{"title":"Effect of polymer concentration in co-flowing viscoelastic separation of submicron particles","authors":"Samith Hettiarachchi , Dan Yuan , Nam-Trung Nguyen , Jun Zhang","doi":"10.1016/j.talanta.2025.128956","DOIUrl":"10.1016/j.talanta.2025.128956","url":null,"abstract":"<div><div>Manipulation and separation of submicron particles such as extracellular vesicles (EVs), viruses and bacteria have broad applications in biotechnology and diagnostics. Viscoelastic microfluidic technology has emerged as a powerful technique for high-resolution particle sorting in non-Newtonian fluids. Viscoelastic co-flowing system is the most popular design for particle separation in viscoelastic microfluidics, and has been successfully employed for the separation of EVs, bacteria and cancer cells. However, current studies mainly focus on particle differential migration in viscoelastic fluids of low polymer concentration, and the effects of high polymer concentration on particle migration and separation are still largely unexplored. In this work, we investigate the migration behaviour of 100 nm and 500 nm particles in a viscoelastic co-flowing microfluidic system of high polyethylene oxide (PEO) concentrations. The effects of PEO concentration of sample and sheath flows, the flow rate ratio (FRR) of the sheath to sample flows and the total flow rate on the particle migration and final equilibrium positions were studied. At low PEO concentrations, large (500-nm) particles migrate fast toward the channel centre and small (100-nm) particles exhibit slow migration. In contrast, at specific high PEO concentrations, an intriguing reversed phenomenon appears where small (100-nm) particles migrate fast and focus at the channel centre, while 500-nm particles remain near sidewalls. Finally, we successfully applied this phenomenon for the separation of binary submicron particle mixture and achieved separation purities of 88% and 87% for 100-nm and 500-nm particles, respectively. This work demonstrates the potential for optimising size-based submicron particle separation in the co-flowing system by tuning high polymer concentrations.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 ","pages":"Article 128956"},"PeriodicalIF":6.1,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145236280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conductive hydrogel with double network structure for robust and flexible wearable sensors. 双网状结构导电水凝胶，用于坚固灵活的可穿戴传感器。

IF 6.1 1区化学

Talanta Pub Date : 2025-10-04 DOI: 10.1016/j.talanta.2025.128928

Jingjing Yang, Benfeng Zhu, Sheng Wan, Qingqing Ni

引用次数: 0

A 4D-printed, magnetically actuated automated solid-phase extraction device coupled with ICP-MS for multiple trace metal analysis. 3d打印，磁驱动的自动固相萃取装置与ICP-MS相结合，用于多种痕量金属分析。

IF 6.1 1区化学

Talanta Pub Date : 2025-10-04 DOI: 10.1016/j.talanta.2025.128955

Jhih-Ying Jian, Cheng-Kuan Su

{"title":"A 4D-printed, magnetically actuated automated solid-phase extraction device coupled with ICP-MS for multiple trace metal analysis.","authors":"Jhih-Ying Jian, Cheng-Kuan Su","doi":"10.1016/j.talanta.2025.128955","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128955","url":null,"abstract":"Four-dimensional printing technologies are accelerating the development and fabrication of stimuli-responsive devices for analytical applications. Herein, we employed the multi-material fused deposition modeling printing technique, iron(II,III) oxide nanoparticle-incorporated polylactic acid filaments, and acrylonitrile butadiene styrene filaments to fabricate an all-in-one solid-phase extraction (SPE) device, which featured the flow manifolds connecting a monolithic packing and four temperature-controlled and magnetically actuated switching valves that were actuated by a hammer-shaped cantilever. When the inner chamber of the cantilever was filled with warm water (above the glass transition temperature of the polylactic acid), the applied external magnetic field induced the bending of the cantilever to switch the flow direction. When removing the external magnetic field, the cantilever returned to its original position, allowing recovery of the flow direction for magnetically actuated fluid control. The optimized SPE device manipulated the sample and eluent streams to pass through the monolithic packing and enabled an automated SPE scheme coupled with inductively coupled plasma mass spectrometry for determination of Mn, Co, Ni, Cu, Zn, Cd, and Pb ions, with the method's detection limits ranging from 0.3 to 2.8 ng L-1. We validated the reliability and applicability of this analytical method through determining the metal ions in reference materials (CASS-4, SLRS-5, 1643f, and Seronorm™ Trace Elements Urine L-2) and real samples (seawater, river water, ground water, and human urine). Our results suggest that four-dimensional printing technologies can effectively fabricate thermo-magneto-responsive analytical devices and enhance the practical applicability of conventional SPE schemes for multiple trace metal analysis.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt A","pages":"128955"},"PeriodicalIF":6.1,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generating a chemical database of organic nanomers and applying active learning to predict HOMO, LUMO and band gap: Accelerating optoelectronic nanopolymer materials discovery. 建立有机纳米化学数据库并应用主动学习预测HOMO、LUMO和带隙：加速光电纳米聚合物材料的发现。

IF 6.1 1区化学

Talanta Pub Date : 2025-10-04 DOI: 10.1016/j.talanta.2025.128939

Qin Zhu, Yanwei Tang, Xinyao Ge, Chong Zhang, Xun Fu, Yongxia Wang, Dong Jin, Lizhu Dong, Jinyi Zhang, Qiang Zhao, Ying Wei, Xiaogang Cheng, Linghai Xie

{"title":"Generating a chemical database of organic nanomers and applying active learning to predict HOMO, LUMO and band gap: Accelerating optoelectronic nanopolymer materials discovery.","authors":"Qin Zhu, Yanwei Tang, Xinyao Ge, Chong Zhang, Xun Fu, Yongxia Wang, Dong Jin, Lizhu Dong, Jinyi Zhang, Qiang Zhao, Ying Wei, Xiaogang Cheng, Linghai Xie","doi":"10.1016/j.talanta.2025.128939","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128939","url":null,"abstract":"Organic nanogrids are versatile molecular hornstones and nanoplatforms of organic high-dimensional, low-entropy materials. It is urgent to construct virtual databases of organic nanomers for accelerating the discovery and performance optimization of novel 0/1/2/3-dimensional nanopolymer optoelectronic materials. In this study, we generated a comprehensive dataset of 11,224 ladder-type gridarenes, covering a wide range of chemical compositions and structural variations. A random selection of 220 small sample sets was aggregated, and fragment-level constrained density functional theory (CDFT) was employed to extract molecular descriptors. These descriptors were then used to train machine learning models with high predictive accuracy for band gap, highest occupied molecular orbital (HOMO), and lowest unoccupied molecular orbital (LUMO) energies (the coefficient of determination values of 0.94, 0.92, and 0.87, respectively). During the active learning process, 3112 representative gridarenes were iteratively selected from our 11,224-compound library, refining band-gap predictions to a mean absolute error below 0.11 eV. This process pinpointed top candidates for blue-light emission and demonstrated an accelerated, data-driven route to next-generation organic optoelectronic nanomaterials.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt A","pages":"128939"},"PeriodicalIF":6.1,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of MOF-808 based dispersive solid-phase extraction and ultra-high performance liquid chromatography tandem mass spectrometry for monitoring organophosphate diesters in urine 基于MOF-808分散固相萃取和超高效液相色谱串联质谱法监测尿液中有机磷二酯的研究

IF 6.1 1区化学

Talanta Pub Date : 2025-10-03 DOI: 10.1016/j.talanta.2025.128950

Meng Yu , Chang Xie , Ding Chen , Qitong Xu , Jun Li , Huawei Wang , Surong Mei

{"title":"Development of MOF-808 based dispersive solid-phase extraction and ultra-high performance liquid chromatography tandem mass spectrometry for monitoring organophosphate diesters in urine","authors":"Meng Yu , Chang Xie , Ding Chen , Qitong Xu , Jun Li , Huawei Wang , Surong Mei","doi":"10.1016/j.talanta.2025.128950","DOIUrl":"10.1016/j.talanta.2025.128950","url":null,"abstract":"<div><div>Urinary organophosphate diesters (di-OPEs) serve as characteristic biomarkers for human exposure to organophosphate triesters (tri-OPEs). Existing sample pretreatment technologies for simultaneously extracting various di-OPEs in urine may be affected by the limited specific interactions and complicated urinary matrix interference, resulting in suboptimal recoveries. To establish a high-throughput and multi-target tool for monitoring urinary di-OPEs, zirconium-based metal organic framework MOF-808 was hydrothermally fabricated as dispersive solid-phase extraction (DSPE) adsorbents, and coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for quantification. MOF-808 exhibited maximum adsorption capacities of 108∼133 mg g−‍1 for target di-OPEs with a rapid adsorption equilibrium time of 30 min. The calculated adsorption energies between MOF-808 and di-OPEs ranged from −56.7 to −87.1 kJ mol−1, which were primarily driven by the synergistic effects of hydrogen bonding and π-π stacking. Under determined DSPE parameters, the developed DSPE-UPLC-MS/MS technology showed ideal linearity ranged from 0.05 to 50.0 ng mL−‍1, with limits of detection (LODs) of 0.02–0.45 ng mL−‍1, spiked recoveries of 75.6 %∼121.8 %, and matrix effects of 67.9 %∼121.0 %. This technology was successfully validated through the detection of twelve di-OPEs in 24-h pooled urine samples from college students, demonstrating the detection frequencies of 13.9 %–100 %, and specific gravity (SG)-adjusted median concentrations ranging from 0.09 to 7.62 ng mL−1. Notably, six urinary di-OPEs exhibited significantly higher levels in females than males (P < 0.05). Overall, this newly proposed DSPE-UPLC-MS/MS technology enabled simultaneous quantification of twelve di-OPEs in urine.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 ","pages":"Article 128950"},"PeriodicalIF":6.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145236347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep-learning-driven spectral image analysis for intelligent monitoring of multiple pesticides and antibiotics. 基于深度学习驱动的光谱图像分析，用于多种农药和抗生素的智能监测。

IF 6.1 1区化学

Talanta Pub Date : 2025-10-03 DOI: 10.1016/j.talanta.2025.128942

Zhizhi Fu, Lu Liu, Qiannan Duan, Liulu Yao, Qianru Wan, Chi Zhou, Weidong Wu, Fei Wang, Jianchao Lee

{"title":"Deep-learning-driven spectral image analysis for intelligent monitoring of multiple pesticides and antibiotics.","authors":"Zhizhi Fu, Lu Liu, Qiannan Duan, Liulu Yao, Qianru Wan, Chi Zhou, Weidong Wu, Fei Wang, Jianchao Lee","doi":"10.1016/j.talanta.2025.128942","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128942","url":null,"abstract":"With the widespread use of pesticides and antibiotics in agriculture and healthcare, their associated environmental pollution and potential health hazards have emerged as a global concern. This study presents a novel deep learning-based spectral image analysis approach that is dedicated to the intelligent monitoring of multiple pesticides and antibiotics in agricultural water bodies. A total of 6100 samples containing glyphosate (GL), bentazone (BE), benzylpenicillin potassium (BP), and tetracycline hydrochloride (TH) at concentrations range of 3.8-550 μg/L were prepared. After the samples were mixed with selected composite chromogenic reagents, the specific absorbance characteristics of the stabilized reaction mixtures were measured using a custom-designed spectrometer. The preprocessed spectral data were used to train a fine-tuned ResNet-50 deep learning model. By establishing mappings between spectral features and reference concentrations, the model effectively predicted unknown pollutant concentrations. The results indicated that the proposed method enables rapid and simultaneous detection of GL, BE, BP and TH. Under laboratory conditions, the coefficient of determination exceeded 0.993, the reliable prediction rate was over 80 % in the concentration range of 10-550 μg/L. The limits of detection for GL, BE, BP, and TH were 0.23, 0.32, 0.38, and 0.28 μg/L, respectively. In addition, the frequency of abnormal predictions for natural water samples exhibited an increase over the concentration range of 3.8-10 μg/L, while the overall accuracy remained relatively high. Our research provides a new perspective on the rapid identification of pesticides and antibiotics. In the future, we hope this method can offer a timely, cost-effective and scalable solution for the early warning and real-time tracking of pollutants in water bodies.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt A","pages":"128942"},"PeriodicalIF":6.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative analysis of Pb²⁺ ion in liquid by laser induced breakdown spectroscopy using chelating agent for sample preparation. 用螯合剂制备样品，激光诱导击穿光谱法定量分析液体中铅离子。

IF 6.1 1区化学

Talanta Pub Date : 2025-10-03 DOI: 10.1016/j.talanta.2025.128941

Haosheng Dong, Endian Hou, Yuting Bai, Nan Zhang, Bing Liu, Weiwei Liu

{"title":"Quantitative analysis of Pb2+ ion in liquid by laser induced breakdown spectroscopy using chelating agent for sample preparation.","authors":"Haosheng Dong, Endian Hou, Yuting Bai, Nan Zhang, Bing Liu, Weiwei Liu","doi":"10.1016/j.talanta.2025.128941","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128941","url":null,"abstract":"Lead is a highly toxic element which poses a serious threat to human health when it dissolves in water. Laser induced breakdown spectroscopy is a simple and fast element detection method which can be used to detect liquid samples. In order to improve the limit of detection of Pb2+ in water, chelating agent assisted LIBS is investigated in this work. Sodium diethyldithiocarbamate (DDTC) is a commonly used Pb2+ chelating agent which can chelate Pb2+ bidentately. In experiments, sodium DDTC is used to chelate Pb2+ and deposits at the bottom of liquid sample by centrifugation. The precipitate is dried and concentrated on the graphite substrate. Under the optimal conditions, such as optimal concentration of sodium DDTC (0.25 mg/mL) and centrifugation time (5 min), the limits of detection of Pb2+ are 2.82 ng/mL for tap water and 3.64 ng/mL for river water are achieved. Without using sodium DDTC, the limits of detection of Pb2+ for tap water and river water are 18.20 ng/mL and 23.00 ng/mL respectively. Sodium DDTC can improve the limit of detection by more than 6 times. The work in this paper proposes a fast, simple and cost-effectively method to quantitatively measure heavy metallic elements in water samples.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt A","pages":"128941"},"PeriodicalIF":6.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A biomimetic folding paper co-culture system for analyzing cellular crosstalk in bone healing: Interactions of bone morphogenetic protein-2, Wnt family member 1, periostin, and platelet-derived growth factor subunit A between periosteum-derived progenitor cells and adipose-derived stem cells. 用于分析骨愈合中细胞串音的仿生折叠纸共培养系统：骨膜源性祖细胞和脂肪源性干细胞之间骨形态发生蛋白-2、Wnt家族成员1、骨膜蛋白和血小板衍生生长因子亚单位A的相互作用

IF 6.1 1区化学

Talanta Pub Date : 2025-10-02 DOI: 10.1016/j.talanta.2025.128943

Felice Prawira, Clarissa Alexandra, Yun-Wen Tong, Pietradewi Hartrianti, Almitra Rindiarti, Alvin Chao-Yu Chen, Kin Fong Lei

{"title":"A biomimetic folding paper co-culture system for analyzing cellular crosstalk in bone healing: Interactions of bone morphogenetic protein-2, Wnt family member 1, periostin, and platelet-derived growth factor subunit A between periosteum-derived progenitor cells and adipose-derived stem cells.","authors":"Felice Prawira, Clarissa Alexandra, Yun-Wen Tong, Pietradewi Hartrianti, Almitra Rindiarti, Alvin Chao-Yu Chen, Kin Fong Lei","doi":"10.1016/j.talanta.2025.128943","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128943","url":null,"abstract":"Bone defects, commonly associated with osteoporosis, result in fragile bones prone to fractures in both men and women. Osteoporotic fractures often lead to prolonged healing due to impaired cell differentiation. Understanding cellular crosstalk during bone regeneration is crucial for developing effective treatments. Periosteum-derived progenitor cells (PDPCs) and adipose-derived stem cells (ADSCs) play essential roles in bone formation because ADSCs secret key growth factors, such as bone morphogenetic protein-2, Wnt family member 1, Periostin, and platelet-derived growth factor subunit A, that promote osteogenesis. To investigate cellular interactions in bone healing process in the natural bone microenvironment, we developed a biomimetic folding paper co-culture system that mimics the inflamed three-dimensional bone structure. This system enables co-culture of PDPCs and ADSCs, allowing the study of their protein crosstalk and osteogenic potential. Moreover, neutralizing assays were conducted to inhibit specific cytokines and evaluate their influence on osteogenesis. Our findings confirm that ADSCs promote osteogenesis through their secreted growth factors that enhance the differentiation and activity of PDPCs. Disrupting these cellular interactions through cytokine inhibition led to a significant reduction in osteogenic potential. It was evidenced by decreased protein expression and gene activation associated with bone formation. This biomimetic folding paper co-culture system effectively mimics the natural bone microenvironment and provides a novel platform to study bone healing mechanisms. This approach may lead to the development of more effective treatments for bone fractures.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt A","pages":"128943"},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential-resolved electrochemiluminescence multiplex immunoassays with isolated cathodic and anodic co-reactants and pre-oxidized Ag-doping methionine-stabilized Au nanoclusters. 电位分辨电化学发光多重免疫分析，分离阴极和阳极共反应物和预氧化ag掺杂蛋氨酸稳定的金纳米团簇。

IF 6.1 1区化学

Talanta Pub Date : 2025-10-02 DOI: 10.1016/j.talanta.2025.128927

Qiao Zhang, Ruoqi Wang, Lei Yu, Yunwei Gao, Dazhong Shen

{"title":"Potential-resolved electrochemiluminescence multiplex immunoassays with isolated cathodic and anodic co-reactants and pre-oxidized Ag-doping methionine-stabilized Au nanoclusters.","authors":"Qiao Zhang, Ruoqi Wang, Lei Yu, Yunwei Gao, Dazhong Shen","doi":"10.1016/j.talanta.2025.128927","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128927","url":null,"abstract":"In this work, a potential-resolved electrochemiluminescence (ECL) multiplex immunoassay (MIA) was developed using Ag-doping methionine-stabilized Au nanoclusters (Met-AuAgNCs) with immobilized co-reactant as the anodic ECL tag and nanocomposite of gold nanoparticles/graphene oxide/N, N'-dicaproate sodium-3,4,9,10-perylene-dicarboximide (AuNPs/GO/PDI) as the cathodic ECL tag. Compared with methionine-stabilized Au nanoclusters (Met-AuNCs), the ECL of Met-AuAgNCs was enhanced 5.61-fold. When anodic co-reactant of N,N-diethylethylenediamine (DEDA) was connected to Met-AuAgNCs, the ECL of DEDA-Met-AuAgNCs was 11.3-fold of that of Met-AuAgNCs in DEDA solution due to the shorter charge transfer distance between Met-AuAgNCs and DEDA. After a pre-oxidation at 0.95 V for 60 s, the ECL of DEDA-Met-AuAgNCs was further enhanced by 10.6- and 27.9-fold in the cyclic voltammetric and potential step modes, respectively. The pre-oxidation ECL enhancement was demonstrated by an immobilized co-reactant promoters mechanism. In a potential-resolved ECL-MIA, carbohydrate antigen 125 and carbohydrate antigen 19-9 were adopted as model analytes, with the detection limits of 0.029 and 0.076 mU mL-1, respectively. The work provides a proof of concept using self-ECL luminophores with immobilized co-reactant promoters in situ formed for potential-resolved ECL-MIAs with isolated anodic and cathodic co-reactants.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt A","pages":"128927"},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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