Talanta最新文献

{"title":"X-ray fluorescence analysis of contemporary metal sculptures.","authors":"Leonardo S G Teixeira, Gianni Gallello, Miguel de la Guardia","doi":"10.1016/j.talanta.2026.130167","DOIUrl":"10.1016/j.talanta.2026.130167","url":null,"abstract":"<p><p>A portable X-ray fluorescence spectrometer (pXRF) has been employed to study the composition of contemporary metal sculptures. The equipment calibration was verified against a reference material and by using circulating coins with compositions declared by central banks. It was shown that coins can serve as a cheaper alternative for verifying the accuracy of XRF measurements. The analyses of 14 sculptures revealed that the same object can exhibit parts with markedly different compositions, particularly when these parts are independent and perform different functions within the artwork. On the other hand, a tendency towards compositional homogeneity was observed across the main body of the same artwork, consistent with the use of a single metal alloy in the sculpture's manufacture. Furthermore, it was not possible to establish unequivocal compositional characteristics for works attributed to the same artist, and it was found that certified limited-edition sculptures exhibited high-quality bronze, whereas other works did not adhere to a specific metal-alloy standard, reflecting different manufacturing processes. It has been confirmed that compositional analysis of sculptures using pXRF can be important for the certification and authentication of these artworks, as well as for evaluating material quality and potential restorations.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130167"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Competitive kinetic mechanisms in one-pot isothermal amplification-CRISPR systems: From model construction to performance evaluation.","authors":"Hao Jiang, Junyuan Yang, Anyi Li, Di Mou, Yulin Deng, Xuefei Lv","doi":"10.1016/j.talanta.2026.130184","DOIUrl":"10.1016/j.talanta.2026.130184","url":null,"abstract":"<p><p>The urgent demand for integrating real-time molecular diagnosis with isothermal amplification and CRISPR-based detection has underscored the critical need for streamlined, single-tube one-pot methodologies. However, such integration is often hindered by temporal incompatibilities, including premature activation of early amplification products by CRISPR components, which leads to primer degradation and reduced amplification efficiency. To address this challenge, we established a dedicated quantitative competitive kinetic framework for enzyme-free isothermal amplification-CRISPR one-pot systems, using hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) as representative models. Two core inhibitory mechanisms were identified: \"pre-activation-degradation inhibition\", in which early CRISPR activation degrades amplification intermediates, and \"substrate competition inhibition\" in which hairpin probes compete with reporter probes for CRISPR trans-cleavage. Corresponding kinetic equations were derived to describe these interactions quantitatively. Systematic experimental validation of key parameters such as DNA activator concentration, reporter probe concentration, hairpin probe concentration, and ribonucleoprotein (RNP) complex concentration, confirmed the reliability and predictive accuracy of the proposed models. These results provide mechanistic insights into the factors governing one-pot HCR/CHA-CRISPR coupling and identify conditions that optimize assay performance. Overall, this study offers a theoretical foundation and experimental guidance for probe design, reaction condition optimization, and sensitivity enhancement in enzyme-free isothermal amplification-CRISPR one-pot platforms, and provides a general reference for the rational integration of isothermal amplification and CRISPR-based detection.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130184"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to' MXene/zinc ion embedded agar/sodium alginate hydrogel for rapid and efffcient sterilization with photothermal and chemical synergetic therapy' [Talanta 266 (2024) 125101-125109/214574].","authors":"Yuwei Hu, Qin Zeng, Yifan Hu, Jinyun He, Heye Wang, Chunyan Deng, Dai Li","doi":"10.1016/j.talanta.2026.130024","DOIUrl":"10.1016/j.talanta.2026.130024","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130024"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Radiocarbon-based direct liquid scintillation counting method to quantify biogenic carbon and fossil-derived synthetic adulterants in commercial essential oils\" [Talanta 296 (2026) 129583].","authors":"K Arya Krishnan, S Bharath, N Karunakara","doi":"10.1016/j.talanta.2026.130127","DOIUrl":"10.1016/j.talanta.2026.130127","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130127"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A thiol-tolerant Au-Se anchored fluorescent aptamer nanosensor for GP73 detection with anti-interference validation and proof-of-concept serum/cellular imaging.","authors":"Lailin Fu, Congcong Xu, Guowen An, Lingyun Wang, Peng Li, Mei Lv, Xiaofei Zhang","doi":"10.1016/j.talanta.2026.130149","DOIUrl":"10.1016/j.talanta.2026.130149","url":null,"abstract":"<p><p>Golgi protein 73 (GP73) is a clinically relevant biomarker associated with hepatocellular carcinoma (HCC). However, fluorescence-based quantification and intracellular imaging are susceptible to interference and signal instability in thiol-rich biological environments. Herein, we report a fluorescent aptamer nanosensor based on Au-Se anchoring, which integrates a mechanistically guided anti-interference design with a calibratable, buffer-based quantitative workflow for GP73 detection. The sensing interface translates GP73 recognition into fluorescence recovery via target-induced aptamer conformational switching and increased fluorophore-gold nanoparticle distance, while the Au-Se bond mitigates thiol-driven false activation. Under optimized conditions, the assay shows a linear response in the range of 0-800 ng/mL with a detection limit of 1.51 ng/mL. Stability and tolerance studies demonstrate its robust performance across a pH range of 6.2-8.0 and in the presence of physiologically relevant concentrations of glutathione. Notably, in 5 mM glutathione, the signal increases by less than 1.5-fold compared to the untreated control, and its performance is significantly superior to that of the conventional Au-S anchored probe. Specificity experiments confirm that the probe responds significantly only to GP73, showing negligible responses to a panel of non-target proteins and a scrambled aptamer control. Preliminary serum experiments further demonstrate feasibility in a 10× diluted human serum matrix, where the probe preliminarily differentiates GP73-positive HCC patient sera from sera of other cancer patients using a ΔF strategy to reduce serum background fluorescence; however, we emphasize that the cohort size is limited and larger clinical validation is required for definitive clinical claims. Cellular experiments reveal GP73-correlated fluorescence contrast between HepG2 and normal liver (THLE-2) cells, and the thiol-depletion control (N-ethylmaleimide) supports that intracellular signal activation is thiol-independent. In summary, this work provides a robust Au-Se-stabilized nanosensor for quantitative GP73 readout in buffer and cellular imaging under thiol-rich conditions. The control experiments clearly define its specificity and anti-interference advantages, delineating key practical operating boundaries and providing a foundation for future expanded validation in complex clinical matrices.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130149"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIR-responsive MnO₂-functionalized mesoporous silica hydrogel: Fabrication and antibacterial activity evaluation.","authors":"Xue Yang, Yu Si, Shasha Jiang, Meng Zhuo, Tong Ren, Zijun Han, Xuetong Zhao, Xueyan Hou, Yongli Shi","doi":"10.1016/j.talanta.2026.130186","DOIUrl":"10.1016/j.talanta.2026.130186","url":null,"abstract":"<p><p>In this study, an 808 nm near-infrared (NIR)-responsive hydrogel (NOR-MSN@MnO₂-gel) was developed for the treatment of bacteria-infected wounds. The norfloxacin (NOR)-loaded manganese dioxide-modified mesoporous silica (NOR-MSN@MnO₂) nanoparticles were selected as a photothermal material and encapsulated in the furfuryl amine-modified hyaluronic acid hydrogel (HA-FA^gel). Studies found that MSN@MnO₂-gel exhibited a three-dimensional porous network structure, favorable injectability, excellent water absorption & retention capacity, and desirable photothermal conversion efficiency. Benefiting from pH/NIR dual-responsive characteristics, NOR-MSN@MnO₂-gel realized controllable NOR release and achieved efficient elimination of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) through synergistic photothermal therapy (PTT) and chemotherapy. In vivo infected wound studies demonstrated that NOR-MSN@MnO₂-gel combined with NIR irradiation (NOR-MSN@MnO₂-gel + NIR) could effectively inhibit bacterial growth, alleviate inflammation, accelerate re-epithelialization, and promote collagen deposition & angiogenesis, while maintaining satisfactory biological safety. Collectively, the NOR-MSN@MnO₂-gel + NIR regimen provides a promising strategy for clinical treatment of bacterial infected wounds.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130186"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-aided Cu₃(HHTP)₂/COOH-MWCNTs electrochemical sensing platform for simultaneous detection of dopamine and uric acid in human serum.","authors":"Zihao Zhao, Jianlong Li, Jinmi Zhang, Qiang Li, Sheng Li, Yingbo Shi, Xiaoli Xiong","doi":"10.1016/j.talanta.2026.130163","DOIUrl":"10.1016/j.talanta.2026.130163","url":null,"abstract":"<p><p>The simultaneous determination of dopamine and uric acid in complicated biological matrices remains challenging due to overlapping electrochemical signals and interference from coexisting species. Herein, a machine learning-assisted electrochemical sensor based on a Cu₃(HHTP)₂/COOH-MWCNTs hybrid is developed for the simultaneous determination of these analytes. Benefiting from the unique π-π stacking and π-d conjugation characteristics of Cu₃(HHTP)₂, as well as the excellent coordination effect between COOH-MWCNTs and Cu²⁺ and the good dispersibility of carbon nanotubes themselves, the composite material exhibits more prominent surface activity, significantly boosting the exposure of active sites and accelerating the electron transfer kinetics at the electrode-electrolyte interface. On this basis, we constructed an electrochemical sensing platform for the simultaneous detection of DA and UA in human serum samples. The sensor show excellent performance with dual linear ranges of 0.5-15 and 15-80 μM for DA, and 1-200 and 200-350 μM for UA, achieving low LODs of 0.039 μM and 0.083 μM, respectively (S/N = 3). Furthermore, machine learning (ML) algorithms were employed to optimize electrochemical signal processing, enabling more accurate and robust analysis of complex data. The proposed approach achieved successful application in the simultaneous determination of DA and UA in human serum matrix samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"310 ","pages":"130163"},"PeriodicalIF":6.7,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterojunction-induced interface barrier modulation in ZnO@Zn-HHTP for dopamine detection.","authors":"Zhanglin Weng, Zhijie Xie, Zhonghui Chen, Cuiying Lin, Bin Qiu, Chuanhui Huang, Zhenyu Lin","doi":"10.1016/j.talanta.2026.130804","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130804","url":null,"abstract":"<p><p>Rational modulation of interfacial charge-transfer processes is critical for advancing the performance of electrochemical sensors, yet precise control of heterojunction-induced potential barriers for selective molecular recognition remains challenging. Herein, we report a ZnO@Zn-2,3,6,7,10,11-hexahydroxytriphenylene (HHTP) core-shell heterostructure that enables highly selective detection of dopamine (DA) via analyte-responsive modulation of an interfacial potential barrier. The heterostructure is synthesized through an in-situ conversion strategy, transforming a ZIF-8 shell on ZnO nanorods into Zn-HHTP while preserving the core-shell architecture. The resulting ZnO/Zn-HHTP interface forms a well-defined n-p heterojunction with a built-in potential barrier that governs charge-transfer kinetics. Positively charged DA induces interfacial charge redistribution at the negatively charged Zn-HHTP surface, lowering the barrier height and facilitating electron transfer, thereby amplifying the electrochemical signal. In contrast, common interferents induce negligible response, as they are unable to modulate the interfacial potential barrier and do not undergo effective electrochemical reaction at the characteristic redox potential of dopamine, thereby enabling selective detection. The sensor displays a wide linear range (50 nM-300 μM) and a low detection limit of 16.7 nM, which demonstrates reliable performance in the detection of target in human urine samples. This work highlights interfacial barrier modulation as an effective strategy for designing high-performance electrochemical sensors based on conductive MOF-semiconductor heterostructures.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"313 Pt A","pages":"130804"},"PeriodicalIF":6.7,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bismuth-stabilized perovskites with water-boosted fluorescence for accurate quantification of high-concentration chloride in wastewater.","authors":"Chao Fan, Dongdong Ge, Yaheng Zhang, Qinggang Zhang, Jun Wang, Xiaofan Chang, Shouqiang Huang","doi":"10.1016/j.talanta.2026.130797","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130797","url":null,"abstract":"<p><p>The rapid anion exchange between chloride (Cl^-) and bromide (Br^-), accompanied by remarkable photoluminescence (PL) shifts, enables CsPbBr₃ perovskites to exhibit excellent selectivity for Cl^- detection. However, CsPbBr₃ nanocrystals stabilized by organic ligands usually exhibit limited applicability for Cl^- detection in water, because the hydrophobic ligands prevent direct contact with aqueous Cl^- and restrict interfacial ion-exchange kinetics. Ligand-free CsPbBr₃-based perovskites synthesized by solid-state methods generally suffer from poor stability in aqueous environments and weak fluorescence, arising from their intrinsic ionic nature, abundant defect states, and large grain size, which severely limit their application in fluorescence sensing. Herein, metallic bismuth (Bi) with strong affinity toward Cl^- was introduced to form composites with ligand-free CsPbBr₃/Cs₄PbBr₆ perovskites, constructing multi-branched CsPbBr₃/Cs₄PbBr₆/Bi composites (CPB-Bi) for Cl^- detection in ethanol-water environments. The incorporation of Bi provides a robust structural framework and interfacial protection, significantly improving the stability of perovskite components in polar solvent environments. Additionally, the amount of water in ethanol induces partial reconstruction of Cs₄PbBr₆ into CsPbBr₃ nanocrystals and passivates surface defects, resulting in enhanced radiative recombination and greatly improved PL intensity. Owing to the rapid reaction between the nanosized perovskite materials and chloride ions, the detection time was significantly reduced to 10 min. Based on the Cl^-/Br^- anion exchange mechanism, a linear relationship between PL peak positions and Cl^- concentrations was established. The optimized CPB-Bi10 (10 mmol Bi) sensor achieved a chloride detection accuracy of 95.65% in wastewater samples at Cl^- concentrations up to 10,000 ppm. The sensor exhibits outstanding sensitivity, high selectivity, and robust anti-interference capability, offering a facile strategy for designing ligand-free perovskite-based fluorescent sensors for environmental ion monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"313 Pt A","pages":"130797"},"PeriodicalIF":6.7,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geometry-optimized passive SPME sampler for time-weighted average determination of volatile organic compounds in air: Proof of concept.","authors":"Olga P Ibragimova, Anara Omarova, Diana Sovetova, Akmaral Rakhym, Nassiba Baimatova","doi":"10.1016/j.talanta.2026.130788","DOIUrl":"https://doi.org/10.1016/j.talanta.2026.130788","url":null,"abstract":"<p><p>Accurate time-weighted average (TWA) concentrations are critical for evaluating inhalation exposure, health risks, and pollution trends. Solid-phase microextraction (SPME) offers a sensitive, simple alternative to conventional volatile organic compound (VOC) monitoring methods. Using a gas chromatograph inlet liner as a passive sampler with an internally exposed SPME fiber eliminates analyte adsorption onto the metallic surface, a limitation of retracted-fiber configurations. Based on COMSOL Multiphysics modeling results, a modified sampler was fabricated with an enlarged extraction zone (0.80 mm) and reduced diffusion-path zone (0.34 mm). The 85-μm Car/PDMS fiber was the optimal coating. Corroborating numerical simulations, the modified sampler at 67 mm diffusion path length (Z) with Car/PDMS (91-111%) and MOF-199 (80-94%) fibers achieved higher recoveries than the standard GC liner for all VOCs except perchloroethylene (PCE, 121-145%) and acetophenone (ACP) on MOF-199 (<14%). Extracted mass increased linearly with 1/Z (R² > 0.95, except ACP); Z = 67 mm was selected as optimal, whereas longer paths (97-127 mm) caused overestimation and higher variability for most analytes. Combined kinetic and diffusion-path analyses indicated analyte-dependent responses consistent with coupled gas-phase transport, coating uptake, and sorption effects. The available data did not permit identification of a single rate-controlling mechanism. The method demonstrated exceptional linearity (R² > 0.99), intra-day repeatability (RSD≤14%), inter-day reproducibility (RSD≤23%), spike recoveries of 87-105% (PCE, 123%), and detection/quantification limits of 0.03-0.59 and 0.10-1.99 μg/m³, respectively. During seven 24-h sampling events (March, 2023) in Almaty, Kazakhstan, TWA VOC concentrations were 0.85-38.4 μg/m³. Daily benzene levels remained below Kazakhstan's 24-h limit (100 μg/m³) but exceeded Israel's stricter limit (3.9 μg/m³) by 1.2- to 5-fold on 6 of 7 days.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"313 Pt A","pages":"130788"},"PeriodicalIF":6.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}