Talanta最新文献

{"title":"A malononitrile-based portable fluorescent probe for ultrasensitive detection of hydrazine in environmental matrices","authors":"Lingxiao Xiong ,&nbsp;Wanmeng Li ,&nbsp;Zhiyu Xie ,&nbsp;Bo Wang ,&nbsp;Guodong Feng","doi":"10.1016/j.talanta.2025.128607","DOIUrl":"10.1016/j.talanta.2025.128607","url":null,"abstract":"<div><div>Hydrazine (N₂H₄), a highly toxic environmental pollutant, demands ultrasensitive detection across gaseous, aqueous, and soil matrices to meet the U.S. EPA safety standard (10 ppb). Existing probes rarely integrate portable detection with field-deployable platforms. Herein, a malononitrile-based fluorescent probe (BTN) was developed for ultrasensitive and portable detection of N₂H₄. By integrating a 2,1,3-benzothiadiazole (BTD) fluorophore and malononitrile recognition unit, BTN achieved a remarkable detection limit of 0.023 μM (S/N = 3) with rapid response (20 min) and excellent anti-interference capability. A smartphone-assisted platform enabled on-site liquid-phase detection using test strips (0.1–10 μM via RGB imaging). Simultaneously, BTN demonstrated gas-phase monitoring through fluorescence quenching and tracked degradation dynamics in soil samples. This work pioneers a low-cost, portable strategy for cross-compartment environmental monitoring.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128607"},"PeriodicalIF":5.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in nanomaterial-based electrochemical aptasensors for detecting aquatic toxins in shellfish and freshwater. A review","authors":"Girma Selale Geleta ,&nbsp;Douglas P.M. Saraiva ,&nbsp;Leonardo M.A. Ribeiro ,&nbsp;Jian F.S. Pereira ,&nbsp;Mauro Bertotti","doi":"10.1016/j.talanta.2025.128589","DOIUrl":"10.1016/j.talanta.2025.128589","url":null,"abstract":"<div><div>Aquatic toxins are potent, naturally occurring compounds produced by dinoflagellates, cyanobacteria, and diatoms during harmful algal blooms (HABs) in both freshwater and marine ecosystems. These toxins can accumulate in seafood, such as scallops, oysters, mussels, and certain fish, posing a significant global threat to human health and the environment. The current methods for detecting these aquatic toxins, such as mouse bioassays, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and high-performance liquid chromatography (HPLC), are limited by time-consuming procedures, high costs, and ethical problems. Therefore, there is an immediate need for the advancement of methods that are sensitive, specific, rapid, and affordable for detecting aquatic toxins. Electrochemical biosensors, particularly those incorporating nanomaterials and their composites, exhibit significant potential for enhancing sensitivity due to their large surface area, enhanced catalytic activity, superior electrical conductivity, chemical stability, tunable optical properties, and potential for functionalization. The integration of nanomaterials and their composites with aptamers further enhances the selectivity and specificity of these electrochemical aptasensors. Aptamers, single-stranded oligonucleotides with remarkable affinity and recognition capabilities, are highly valuable in designing nanomaterial-based electrochemical sensors. In comparison to antibodies, aptamers offer numerous benefits, including lower production costs, a smaller molecular weight, enhanced stability, lack of immunogenicity, rapid chemical synthesis, independence from animal sources for their production, ease of transportation, and storage. Therefore, the integration of electrochemical platforms with nanomaterials and aptamers is an effective tool for detecting aquatic toxins, thereby contributing to the protection of public and environmental health. This review focuses on the latest developments in electrochemical aptasensors based on nanomaterials for the detection of aquatic toxins, particularly microcystin-LR (MC-LR), saxitoxins (STX), microcystin-RR (MC-RR), okadaic acid (OA), and cylindrospermopsin (CYN). It explores their fundamental construction principles, methods of signal transduction, performance characteristics, and real-world sample applications of these electrochemical aptasensors in the detection of aquatic toxins. Furthermore, the review addresses existing challenges and outlines future research directions in this vital area of study.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128589"},"PeriodicalIF":5.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A facile photoelectrochemical sensing platform for highly sensitive detection of dopamine secreted from nerve cells based on polydopamine sensitized TiO2 as photoelectric material","authors":"Ji-Cheng Li,&nbsp;Ya-Ni Yuan,&nbsp;Yun Lei,&nbsp;Ai-Lin Liu","doi":"10.1016/j.talanta.2025.128604","DOIUrl":"10.1016/j.talanta.2025.128604","url":null,"abstract":"<div><div>Dopamine (DA) is a catecholamine neurotransmitter in the brain, and changes in its concentration are closely related to neurological diseases. Thus, the development of a sensitive, cost-efficient and reliable method for DA monitoring has important application value. Herein, a photoelectrochemical (PEC) sensor based on TiO₂ and polydopamine-modified TiO₂ (PDA@TiO₂) was constructed to achieve highly sensitive detection of DA secreted from living nerve cells. Upon DA introduction, PDA@TiO₂ has a wide range of visible light absorption, promoting light absorption. The sensitization of PDA effectively improve the charge carrier transport efficiency, promote the photocatalytic activity of TiO₂. The coating of PDA promotes its hydrophilic properties, effectively excluded the biofouling macromolecules and interference signals, ensuring sensor reproducibility. The result reflected that the current reponse increase of PDA@TiO₂ is 3.5 times higher than the TiO₂ after DA reaction due to the PDA@TiO₂ can promote efficient carrier separation. The PDA@TiO₂ sensor has superior sensitivity, a wider detection linear range and long-term stability. Notably, this PEC sensor is characterized by its simplicity in fabrication, low production cost, and portability. Under optimized experimental conditions, the PDA@TiO₂ sensor showed a favorable linear response to DA using PBS incubated with pheochromocytoma (PC12) cells concentration ranging from 2 to 500 μM with a detection limit of 24.7 nM (S/N = 3). Meanwhile, the sensor was effectively applied to detect DA levels in PC12 cells. This work not only provides a new and efficient strategy for signal amplification, but also provides a promising candidate platform for sensitive detection of DA, offering a valuable tool for neuroscience and clinical diagnostics.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128604"},"PeriodicalIF":5.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in metal complex-based colorimetric sensors","authors":"Zhongmei Chi,&nbsp;Shiqi Chu,&nbsp;Bingqian Wang,&nbsp;Zhong Zhang,&nbsp;Guocheng Liu,&nbsp;Xiuli Wang","doi":"10.1016/j.talanta.2025.128591","DOIUrl":"10.1016/j.talanta.2025.128591","url":null,"abstract":"<div><div>Metal complexes and their composites have recently emerged as the pivotal elements of colorimetric analysis, driving substantial progress in sensor technology. Owing to their advantageous optical properties, metal complex-based colorimetric sensors are well suited for detecting various analytes. The sensing properties of these composites can be tailored to specific analytes by varying the metal center (transition metal, lanthanide, or main-group metal ion). Compared with metal complexes, metal complex-based composites achieve markedly higher sensitivities and stabilities in colorimetric sensing, primarily because of the synergistic integration of metal complexes with diverse nanomaterials, including metals, metal oxides, and carbon-based materials. Despite the considerable progress in this field, the structural design of metal complexes and the preparation and performance regulation of their composites remain challenging. Herein, we review the metal complex-based colorimetric sensors developed in the past decade from the perspectives of metal centers, composite nanomaterials, colorimetric mechanisms, and applications, highlighting the need for further research to foster the advancement and utilization of colorimetric sensing based on metal complexes and their composites.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128591"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of lysozyme in human saliva by CE-UV: A new simple, fast, and reliable method","authors":"Radovan Tomašovský ,&nbsp;Martina Opetová ,&nbsp;Michaela Polačková ,&nbsp;Katarína Maráková","doi":"10.1016/j.talanta.2025.128605","DOIUrl":"10.1016/j.talanta.2025.128605","url":null,"abstract":"<div><div>Many studies have shown a correlation between salivary lysozyme levels and the presence of various diseases or stress. Here, we developed a new, simple method for the analysis of lysozyme in human saliva by capillary electrophoresis (CE) and UV detection that has potential application in clinical settings. The optimized method used 1 M formic acid +10 % (v/v) isopropyl alcohol as a background electrolyte, transient isotachophoresis as a preconcentration method, deactivation of the glass insert surface by carbonic anhydrase as a blocking agent to eliminate non-specific protein adsorption, and only simple dilution as a sample pretreatment. The validation showed high linearity (r² = 0.9997), inter- and intra-day accuracy (−2.03 to +5.36 %) and precision (&lt;5 % RSD), and limit of detection (0.5 μg/mL). The stability of lysozyme in human saliva stored at −80 °C for 7 days using protease inhibitors and the variations of lysozyme excretion during the day and over 3 consecutive days were also investigated. The optimized and validated method was applied for the determination of salivary lysozyme levels in 25 healthy volunteers and the results showed the mean salivary lysozyme concentration at 14.3 ± 10.5 μg/mL. To the best of our knowledge, this is the first CE-UV method for human salivary lysozyme determination. This method meets the criteria of simplicity (minimum steps in sample pretreatment and analysis), speed (the entire process from saliva collection to result takes less than 45 min), reliability (highly satisfactory validation data), and suitability for application to real-world samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128605"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of CD81 in mouse serum-derived extracellular vesicles via inductively coupled plasma – mass spectrometry using gold-based labels","authors":"Jaime Martínez-García,&nbsp;Beatriz Fernández,&nbsp;Rosario Pereiro","doi":"10.1016/j.talanta.2025.128609","DOIUrl":"10.1016/j.talanta.2025.128609","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) released by cells are one of the main mechanisms of intercellular communication. Nowadays it is known that they can play important roles in diseases such as cancer or neurodegenerative processes. Therefore, it is of high interest to develop proper procedures for analyzing EVs in order to exploit their potential to understand biological dysregulations and identifying biomarkers, such as proteins. However, the determination of specific proteins in EVs typically require highly sensitive methods, particularly when working with limited sample volumes, such as mouse serum. The combined use of metal labelled immunoprobes and detection by inductively coupled plasma – mass spectrometry (ICP-MS) has proved to be an advantageous strategy for the analysis of proteins in biological samples. In this work, a methodology based on an immunoassay using antibodies labelled with gold nanoclusters, to achieve a high signal amplification, and ICP-MS detection was developed to determine target proteins in EVs from mouse blood serum. As a proof of concept, CD81, a generic marker of EVs, was investigated, and a limit of detection as low as 56 fM was obtained. CD81 levels in EVs isolated from aliquots of 0.5 mL of commercial mouse blood serum were determined after applying an optimized differential centrifugation protocol. Results obtained with the developed analytical procedure were in good agreement with those achieved with a commercial ELISA kit.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128609"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low temperature triethylamine sensing based on annealed amorphous cobalt tin oxide nanoboxes with tunable oxygen vacancies","authors":"Jian Fang ,&nbsp;Wei Wang ,&nbsp;Yizhuo Fan ,&nbsp;Dali Liu ,&nbsp;Yu Chen ,&nbsp;Shengping Ruan","doi":"10.1016/j.talanta.2025.128603","DOIUrl":"10.1016/j.talanta.2025.128603","url":null,"abstract":"<div><div>The development of triethylamine (TEA) sensors with high sensitivity and selectivity is of great significance for realizing multi-field applications such as environmental monitoring and industrial safety. In this study, a novel and high-performance TEA sensor strategy based on single-component amorphous materials is proposed. By employing CoSn(OH)₆ nanocubes as precursors, amorphous hollow CoSnO₃ nanoboxes were successfully synthesized via an alkali etching process followed by a controlled annealing procedure. The results showed that annealing temperature accurately regulated the degree of amorphous and oxygen vacancy concentration of the material. Based on the analysis of characterization test results, it was confirmed that the unique hollow nanobox structure formed through alkali etching significantly enhanced the specific surface area of the pristine nanocube, reaching up to 262.4 m²/g. Gas sensitive tests demonstrated that the response value (Rg/Ra = 24.5) of the HCSO-200 sensor to 50 ppm TEA at low operating temperatures (120^oC) was nearly 4.2 times higher than that of crystalline material, with excellent repeatability and long-term stability. Further mechanistic studies showed that the excellent TEA sensing performance of the HCSO-200 material could be attributed to its amorphous structure, hollow morphology, and optimized oxygen vacancy concentration by controlling annealing temperature. This work not only provides an efficient one-component sensing material for TEA detection, but also broadens the idea of other amorphous semiconductor materials for gas detection.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128603"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gel-free capillary zone electrophoresis at acidic pH for micro DNA and RNA analysis","authors":"Jakob Haglöf ,&nbsp;Erik Bivehed ,&nbsp;Cari Sänger-van de Griend","doi":"10.1016/j.talanta.2025.128610","DOIUrl":"10.1016/j.talanta.2025.128610","url":null,"abstract":"<div><div>Short DNA and RNA sequences like therapeutic anti-sense oligonucleotides (ASO), microDNA (miDNA) and microRNA (miRNA) are predominantly analyzed using liquid chromatographic techniques or capillary gel electrophoresis. The current demand for improved analysis of these nucleotides gives incentives to reconsider old truths, such as the need for gel-based capillary electrophoresis for DNA and RNA analysis.</div><div>The present study shows the applicability of capillary zone electrophoresis using acidic background electrolytes for the separation of ASO-, miDNA- and miRNA-sized nucleotides. Using the different pKa of the nucleobases as a foundation, different nucleotides with same size, but varying sequence, has been successfully separated with high efficiency and short analysis time. Furthermore, the ratio of adenosine and cytidine to guanosine and thymidine nucleobase content, AC/GT, is shown as a valid parameter to assess single-stranded nucleotide migration and separation.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128610"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic chip coupled with photoelectrochemical/fluorescence dual-modal sensing system for the efficient enrichment and detection of circulating tumor cells","authors":"Qingfeng Lin ,&nbsp;Xiaoyang Chen ,&nbsp;Yawen Hong ,&nbsp;Jingyu Huang ,&nbsp;Jiuhong Li ,&nbsp;Yanying Wang ,&nbsp;Lei Chen ,&nbsp;Chunya Li ,&nbsp;Gang Luo","doi":"10.1016/j.talanta.2025.128613","DOIUrl":"10.1016/j.talanta.2025.128613","url":null,"abstract":"<div><div>Conventional percutaneous lung biopsy and imaging modalities are often associated with adverse effects and may yield false-negative results, limiting their clinical efficacy. Circulating tumor cells (CTCs) are pivotal mediators in early tumorigenesis and metastatic dissemination. The detection of CTCs offers unparalleled advantages for early diagnosis and metastasis monitoring. NCI–H460 and NCI–H1650 cell lines serve as representative models for non-small cell lung cancer (NSCLC) and are critical biomarkers given that NSCLC accounts for over 85 % of lung cancer cases. Effective enrichment and precise identification of CTCs from whole blood are essential for early diagnostic applications. Herein, a microfluidic platform integrated with a dual-modal detection system, comprising photoelectrochemical (PEC) sensing and fluorescence imaging, was fabricated for the enrichment and quantification of CTCs (NCI–H460 and NCI–H1650 cells). The Hcy-Thiol probe-labeled CTCs in whole blood were separated by a microfluidic chip, and then a cathodic photoelectrochemical sensing system based on different aptamer-modified CuInS₂ nanoflowers was used to selectively capture CTCs and provide photocurrent signals, thus achieving fluorescence/photoelectrochemical dual-mode detection. Under the optimized conditions, the separation efficiency and purity of CTCs were 83.4 % and 76.8 %, respectively. Quantitative analysis of spiked blood samples at 100 cells/mL yielded cell counts of 107 ± 5 NCI–H460 cells and 87 ± 4 NCI–H1650 cells via photocurrent responses, corroborated by fluorescence signals with counts of 110 ± 4 and 85 ± 3 cells/mL, respectively. This microfluidic integrated with PEC/fluorescence dual-modal sensing system exhibits promising potential for early NSCLC detection and metastasis evaluation with high specificity and sensitivity.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128613"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of desorption electrospray ionization mass spectrometry and mass spectrometry imaging in metabolomics, lipidomics and proteomics analysis","authors":"Haofan Liu ,&nbsp;Sicheng Huang ,&nbsp;Lina Yang ,&nbsp;Yaqian He ,&nbsp;Yongshuai Jing ,&nbsp;Yinghua Xie ,&nbsp;Beibei Hu ,&nbsp;Zhongqiu Li ,&nbsp;Haichao Bi ,&nbsp;Zhiwei Li","doi":"10.1016/j.talanta.2025.128611","DOIUrl":"10.1016/j.talanta.2025.128611","url":null,"abstract":"<div><div>At present, mass spectrometry (MS) and mass spectrometry imaging (MSI) have developed into versatile analytical techniques, applicable for the analysis of both small molecules and macromolecular compounds and complexes. Desorption electrospray ionization (DESI), as an ambient MS (AMS) technique, enables rapid analysis and imaging under open conditions with minimal or no sample preparation required. In this review, the principles and mechanisms of DESI were described, especially for the factors affecting the analysis including suppression effects, device condition parameter settings, and ionization and transmission efficiency. Moreover, the relevant improvements of DESI technique were introduced in detail. Emphasis has been made to discuss the recent research on the application of DESI MS and MSI in the fields of metabolomics, lipidomics, and proteomics. In these applications, DESI MS and MSI exhibit exceptional capabilities for qualitative analysis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"297 ","pages":"Article 128611"},"PeriodicalIF":5.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}