Talanta最新文献

{"title":"Single-molecule resolution of the conformation of polymers and dendrimers with solid-state nanopores.","authors":"Meili Ren, Fupeng Qin, Yue Liu, Daixin Liu, Renata Pereira Lopes, Didier Astruc, Liyuan Liang","doi":"10.1016/j.talanta.2025.127544","DOIUrl":"10.1016/j.talanta.2025.127544","url":null,"abstract":"<p><p>Polymers and dendrimers are macromolecules, possessing unique and intriguing characteristics, that are widely applied in self-assembled functional materials, green catalysis, drug delivery and sensing devices. Traditional approaches for the structural characterization of polymers and dendrimers involve DLS, GPC, NMR, IR and TG, which provide their physiochemical features and ensemble information, whereas their unimolecular conformation and dispersion also are key features allowing to understand their transporting profile in confined ionic nanochannels. This work demonstrates the nanopore approach for the determination of charged homopolymers, neutral block copolymer and dendrimers under distinct bias potentials and pH conditions. The nanopore translocation properties reveal that the dispersion and transporting of PEI is pH-dependent, and its capture rate is much lower than that of PAA. The neutral block copolymer with longest molecular chain threads through with longest blockage duration, its highest capture rate was achieved in 0.5 M KCl at pH 5 with slow diffusion and high temporal resolution. The two generations of neutral dendrimers could also translocate under bias potentials, probably due to the ions adsorption on the dendrimers and driven by Brownian force. The TEG-81 with larger molecular size translocates with longer residence time and higher blockage ratio, as expected. Both of the dendrimers exhibit a higher blockage ratio at pH 7.4 than either acidic or alkalic condition, indicating a larger stretched conformation adopted under neutral condition. This work presents the analysis of unimolecular charged and neutral polymers and dendrimers, which will be insightful in understanding the self-assembly motion and transfer of synthetic macromolecules in confined space. It also provides a good indication for deciphering the macromolecule-nanopore interplay under electrophoretic condition.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127544"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of impurity particles in etching solutions using electrospray-differential mobility analysis","authors":"Duraisamy Senthil Raja,&nbsp;Ching-Hsin Lee,&nbsp;Po-Yu Lai,&nbsp;Ying-Hsuan Wang,&nbsp;De-Hao Tsai","doi":"10.1016/j.talanta.2025.128254","DOIUrl":"10.1016/j.talanta.2025.128254","url":null,"abstract":"<div><div>Particle defects, caused by impurity particles presenting in the chemical solution used in the semiconductor processing, directly impact yield and quality in the semiconductor industry. Early detection and removal of these particles at the chemical supply stage, for example, the etching solution, are crucial. This study presents a new methodology, based on electrospray-differential mobility analysis (ES-DMA), for detecting and quantifying specific organic impurity particles in etching solutions, a highly challenging task with existing methods to date. The results demonstrate that co-solvent with moderate volatility (e.g., H₂O and dimethyl sulfoxide in this study) in the simulated etching solution can be effectively removed for subsequent analysis by generating fine, uniform droplets using electrospray ionization (ES). Using this approach, we successfully identified and quantified model organic impurity particles, including polyethylene glycol (PEG100K; hydrophilic and neutrally-charged) and bovine serum albumin (BSA; amphoteric). The technique achieved detection of ultrafine particles smaller than 10 nm, with a detection limit as low as 10 ppm, even in the presence of high-concentration nonvolatile solutes (e.g., 2 wt% citric acid, used as the representative etchant). These results demonstrate the capability of this ES-DMA method to isolate and analyze specific organic impurity types within complex chemical matrices. Overall, this approach shows strong potential for enhancing quality control in semiconductor manufacturing by enabling early-stage identification of trace-level organic contaminants, leading to better quality control in advanced manufacturing.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128254"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A self-aggregated thermally activated delayed fluorescence nanoprobe for HClO imaging and activatable photodynamic therapy.","authors":"Qingyang Sha, Xinsheng Li, Xixin Gu, Tao Yuan, Jianli Hua","doi":"10.1016/j.talanta.2025.127570","DOIUrl":"10.1016/j.talanta.2025.127570","url":null,"abstract":"<p><p>Hypochlorous acid (HClO/ClO^-) is a common ROS that exhibits elevated activity levels in cancer cells. In this study, an ClO^--triggered TADF probe, PTZ-MNI, was designed based on a naphthalimide core. PTZ-MNI self-assemble in aqueous environments, exhibiting significantly enhanced fluorescence that demonstrated typical aggregation-induced delayed fluorescence (AIDF) characteristics. The probe not only showed high sensitivity to ClO^- but also exhibited remarkable selectivity over other reactive oxygen species and disturbance. PTZ-MNI displayed TADF characteristic, including sensitivity to oxygen in toluene, insensitivity to oxygen in aggregated states that maintain long fluorescence lifetimes, a vertical conformation, and a minimal ΔE_ST of 0.01 eV. Cell imaging studies showed the probe could trace ClO^- by red to green fluorescence in HeLa cell. The colocalization analysis indicated its excellent lysosome-targeting specificity. In addition, PTZ-MNI-O, the compound after oxidation, exhibited effective ROS generation ability and significant PDT effect after irradiation. This work provides guidance for the rational design of responsive TADF luminescent materials used in cell imaging and activatable-PDT.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127570"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial metabolomics platform combining mass spectrometry imaging and in-depth chemical characterization with capillary electrophoresis.","authors":"Leonidas Mavroudakis, Anastasia Golubova, Ingela Lanekoff","doi":"10.1016/j.talanta.2024.127460","DOIUrl":"10.1016/j.talanta.2024.127460","url":null,"abstract":"<p><p>Spatial metabolomics offers the combination of molecular identification and localization. As a tool for spatial metabolomics, mass spectrometry imaging (MSI) can provide detailed information on localization. However, molecular annotation with MSI is challenging due to the lack of separation prior to mass spectrometric analysis. Contrarily, surface sampling capillary electrophoresis mass spectrometry (SS-CE-MS) provides detailed molecular information, although the size of the sampling sites is modest. Here, we describe a platform for spatial metabolomics where MSI using pneumatically assisted nanospray desorption electrospray ionization (PA-nano-DESI) is combined with SS-CE-MS to gain both in-depth chemical information and spatial localization from thin tissue sections. We present the workflow, including the user-friendly setup and switching between the techniques, compare the obtained data, and demonstrate a quantitative approach when using the platform for spatial metabolomics of ischemic stroke.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127460"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriophage-based live bacteria detection for rapid infection diagnosis.","authors":"Merve Calimci, Tugba Tezcan, Emine Kubra Tayyarcan, Kubra Guven, Ismail Hakki Boyaci, Ugur Tamer","doi":"10.1016/j.talanta.2025.127569","DOIUrl":"10.1016/j.talanta.2025.127569","url":null,"abstract":"<p><p>Bacterial bloodstream infections cause high morbidity and mortality. Although bacteria can be detected by various methods, culture methods are often used for the detection of live, accurate, reproducible, and selective bacterial identification. However, the culture method is time-consuming, and clinicians often start treatment immediately due to the long determination time. This reduces the bacterial density detectable by culture, and in some cases, makes determination difficult. To overcome this challenge, we propose a method that directly combines bacteriophage-based lysis with quantitative PCR (qPCR). This method enables the simple and rapid detection of bacteria without the need for pre-concentration or DNA extraction steps. Escherichia coli K12 (E. coli K12) was used as the model bacterium, and bacteria lysed by the E. coli K12-specific bacteriophage were detected using qPCR. The total analysis time was less than 3 h, and only live bacterial cells were selectively lysed. The method was also used to detect bacteria spiked into reference plasma samples, and bacterial DNA was detected via qPCR. The results obtained from the calibration graph created with cultured bacteria and the one created by spiking bacteria into reference plasma were consistent. The similarities between the calibration graphs from both methods were found to be in the range of 92-102.7 %. The LOD and LOQ values for bacteria spiked into reference plasma were calculated as 14.80 and 3.5x10³ CFU/mL, respectively.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127569"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wavelength selection method for near-infrared spectroscopy based on the combination of mutual information and genetic algorithm.","authors":"Xiao-Hui Ma, Zheng-Guang Chen, Shuo Liu, Jin-Ming Liu, Xue-Song Tian","doi":"10.1016/j.talanta.2025.127573","DOIUrl":"10.1016/j.talanta.2025.127573","url":null,"abstract":"<p><p>Near-infrared (NIR) spectroscopy analysis technology has become a widely utilized analytical tool in various fields due to its convenience and efficiency. However, with the promotion of instrument precision, the spectral dimension can now be expanded to include hundreds of dimensions. This expansion results in time-consuming modeling processes and a decrease in model performance. Hence, it is crucial to carefully choose representative features before constructing models. This paper focuses on the limitations of filter algorithms, which can only sort features and cannot directly determine the best subset of features. A hybrid method of combination of the Max-Relevance Min-Redundancy (mRMR) algorithm and the Genetic Algorithm (GA), as well as filter and wrapper feature selection methods, are combined to select appropriate features automatically. This hybrid algorithm retains the features in each individual that are considered to have a strong correlation and low redundancy by the mRMR algorithms during each iteration of the GA. On the other hand, it deletes the features that are regarded as having little correlation or high redundancy. Through the process of iteration, the feature subset is continuously optimized. We use the proposed hybrid method to select features on two datasets and establish various models to verify our proposed method in this paper. The experimental results indicate the feature selection approach, which combines mRMR with the GA, covers the advantages of both feature selection methods. This approach can select features that show good predictive performance. When compared with other common feature selection methods, such as the Uninformative Variable Elimination algorithm (UVE), Competitive Adaptive Reweighted Sampling algorithm (CARS), Successive Projections Algorithm (SPA), Iteratively Retains Informative Variables (IRIV), and GA, the hybrid algorithm can select a larger number of feature variables that are both representative and informative, additionally, it significantly enhances the predictive performance of the model.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127573"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercial reagents-based six-mode sensor: One-step detection of iodide ions without enzymes or nanomaterials","authors":"Mingyue Luo ,&nbsp;Xin Xue ,&nbsp;Kehui Zhang ,&nbsp;Honghong Rao ,&nbsp;Ruibin Qiang ,&nbsp;Panpan Sun ,&nbsp;Marcin Frankowski ,&nbsp;Zhonghua Xue","doi":"10.1016/j.talanta.2025.128253","DOIUrl":"10.1016/j.talanta.2025.128253","url":null,"abstract":"<div><div>Multi-signal sensors possess immense potential for point-of-care testing, yet their widespread adoption is hindered by reliance on complex nanomaterial synthesis or fragile enzymatic systems. Herein, we propose a “six-in-one” multi-mode sensor for rapid iodide ion (I⁻) detection, leveraging only three commercially available reagents: chloroplatinic acid (H₂PtCl₆), hexadecyl trimethylammonium bromide (CTAB), and 3,3′,5,5′-tetramethylbenzidine (TMB). The core innovation lies in a dynamic “on-off-on” oxidation triggered by target I⁻ ions as follows: H₂PtCl₆ with strong oxidative capacity directly oxidizes TMB to its two-electron product (TMB²⁺) (“On” State). CTAB coordinates with H₂PtCl₆ thereby blocking its surface and completely inhibiting TMB oxidation (“Off” State). I⁻ selectively displaces CTAB via competitive coordination, partially restoring H₂PtCl₆'s oxidation activity to generate the one-electron product (TMBox) (“Reactivation” State). Remarkably, the resulting TMBox exhibits six distinct signal outputs including visual and fluorescent color, absorbance, fluorescence, temperature, and electrical current. Compared to conventional single- or dual-mode methods, our six-mode approach improves reliability of detection results. This “magic cube” signals enables dual-mode qualitative analysis and four-mode quantitative detection, achieving a nanomolar-level detection limit for I⁻ detection. Critically, multi-mode sensor operates via one-step mixing without requiring nanomaterials, enzymes, or specialized equipment. We further demonstrate its utility in detecting I⁻ in biological (urine, serum), food (iodized salt) and environmental (Yellow River water, tap water) samples with recovery rates of 96.3 %–110.0 %. Statistical analysis demonstrated excellent reproducibility with relative standard deviations &lt;3.1 % across repeated measurements. This work redefines the paradigm of multi-mode sensing, offering a cost-effective, field-deployable solution for environmental monitoring and clinical diagnostics.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"294 ","pages":"Article 128253"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ratiometric fluorescent probe with dual near infrared emission for in vivo ratio imaging of cysteine.","authors":"Bing Zheng, Shulong Wang, Jiayao Xu, Lixian Huang, Shulin Zhao","doi":"10.1016/j.talanta.2025.127564","DOIUrl":"10.1016/j.talanta.2025.127564","url":null,"abstract":"<p><p>Accurately detecting cysteine (Cys) in vivo is crucial for diagnosing Cys-related diseases. A novel ratiometric fluorescent probe featuring dual near-infrared emission is developed in this study for the in vivo ratio imaging of Cys. The probe comprises a hemicyanine organic small-molecule dye (HCy-CYS) with specific Cys recognition capabilities covalently coupled with carbon dots (CDs) synthesized using glutathione (GSH) as the carbon source (GCDs), forming a unique composite nanofluorescent probe (GCDs@CYS). The probe undergoes a specific reaction with acrylate upon the addition of Cys, converting HCy-CYS to HCy-OH. Consequently, the GCD fluorescence intensity at 685 nm gradually decreases, whereas that of HCy-OH at 720 nm progressively increases, yielding a ratiometric fluorescence signal. Notably, both emission wavelengths of the probe exceed 650 nm, thereby effectively mitigating the interference from background signals during cellular and in vivo imaging. Furthermore, the probe demonstrates high specificity for Cys, enabling its differentiation from homocysteine and GSH. The Cys concentration and fluorescence ratiometric intensity exhibit a strong linear correlation at 10-150 μM with a detection limit of 0.95 μM. These results indicate that the ratiometric fluorescent probe can serve as a valuable tool for monitoring Cys-related physiological or pathological processes.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127564"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High quantum yield copper nanoclusters integrated with nitrogen-doped carbon dots for off-on ratiometric fluorescence sensing of S^2- and Zn².","authors":"Jiang Xue Dong, Kai Xiao, Xiao Li Wu, Zhi Ying Zhong, Hao Yun Yuan, Shi Gang Shen, Ming Su, Na Li, Zhong Feng Gao, Fan Xia","doi":"10.1016/j.talanta.2025.127565","DOIUrl":"10.1016/j.talanta.2025.127565","url":null,"abstract":"<p><p>Pursuing nanomaterials with high fluorescence quantum yields is of great significance in the fields of bioimaging, medical diagnosis, and food safety monitoring. This work reports on orange-emitting aggregation-induced emission (AIE) copper nanoclusters (Cu NCs) integrated with blue-emitting nitrogen-doped carbon dots (N-CDs), which enables highly sensitive detection of S^2- and Zn²⁺ ions through an off-on ratiometric fluorescence method. The highly emissive Cu NCs was doped by Ce³⁺ with a high quantum yield of 51.30 % in aqueous solution. The S^2- can induce fluorescence quenching of AIE Cu NCs/N-CDs from orange to blue, while Zn²⁺ can restore the orange fluorescence. The probe provided linear detection ranges of 0.5-170 μM for S^2- and 0.05-200 μM for Zn²⁺, with detection limits of 0.17 μM and 0.02 μM, respectively. Moreover, a smartphone assistant ratiometric fluorescent test strips were developed for the rapid and visual detection of S^2- and Zn²⁺. The AIE Cu NCs/N-CDs probe exhibited diverse fluorescence color responses, high fluorescence stability, and low cytotoxicity. The ratiometric system was successfully applied to the detection of S^2- and Zn²⁺ in real water samples as well as in cellular and living imaging, demonstrating its potential in biochemical analysis and food safety monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127565"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"A competitive aptamer binding-based CRISPR-cas biosensor for sensitive detection of tetracycline residues in biological samples\" [Talanta, Volume 286 (2025), 127491].","authors":"Yali Zhang, Xingpeng Dong, Cuijuan Jiang, Yanyan Yu, Haiyan Zhang, Jianjie Fu, Gaoxing Su, Yin Liu","doi":"10.1016/j.talanta.2025.127558","DOIUrl":"10.1016/j.talanta.2025.127558","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"127558"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}