IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-05-28 DOI: 10.1016/j.talanta.2025.128344

Selenay Sadak, Gözde Aydoğdu Tığ, Bengi Uslu

{"title":"Design of novel hybrid probe based on double recognition of aptamer-molecularly imprinted polymer-gold nanoparticles for food allergen gliadin sensing.","authors":"Selenay Sadak, Gözde Aydoğdu Tığ, Bengi Uslu","doi":"10.1016/j.talanta.2025.128344","DOIUrl":"10.1016/j.talanta.2025.128344","url":null,"abstract":"Gliadins are allergenic proteins that can pose various risks to human health and are found in high amounts in the most commonly consumed foods. Gliadin, a component of gluten, triggers oxidative stress in celiac disease. Using sensitive analytical methods, especially in foods containing high amounts of these proteins, is crucial for food safety. Aptamer-based biosensors are widely preferred in electroanalytical methods. One of the promising approaches regarding biosensors is the studies in which aptamer-based sensors are combined with molecularly imprinted polymers. The antibody-like binding and ability of MIP to distinguish between molecules increases the method's selectivity. Using a platform modified with aptamer and molecularly imprinted polymer hybrid (MIP) as a new synthetic receptor film, this study presents a selective and sensitive label-free aptasensor for detecting a food allergy gliadin. For this purpose, screen printed gold electrode was used. The electrode surface was electrochemically coated with gold nanoparticles to bind the aptamer to the surface; then the aptamer-gliadin complex was dripped onto the surface and adhered to it, then, o-phenylene diamine monomer was used to synthesize a MIP surface. Gliadin was determined by using differential pulse voltammetry (DPV) in a wide range from 0.25 fg/mL to 1000 pg/mL. The developed method calculated LOD for gliadin determination as 0.011 fg/mL and LOQ as 0.034 fg/mL. The devised aptasensor was not only capable to the discrimination of the commonly found allergen compounds such as bovine serum albumin (BSA), casein, and ara-H1, but also it could detect the gliadin in spiked real samples such as gluten-free bread, crackers, cookies and brown rice cakes samples in the high recovery range of 98.1-104.6 %. The method could be a promising candidate for the sensitive determination of several allergens in food sample analysis.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128344"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating plasma morphology at material boundaries under varying ambient pressures. 研究在不同环境压力下材料边界处的等离子体形态。

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-05-24 DOI: 10.1016/j.talanta.2025.128377

Megha Mohan, David Prochazka, Yi You, Jens Riedel, Igor Gornushkin, Ivana Ročňáková, Martin Papula, Pavel Pořízka, Jozef Kaiser

{"title":"Investigating plasma morphology at material boundaries under varying ambient pressures.","authors":"Megha Mohan, David Prochazka, Yi You, Jens Riedel, Igor Gornushkin, Ivana Ročňáková, Martin Papula, Pavel Pořízka, Jozef Kaiser","doi":"10.1016/j.talanta.2025.128377","DOIUrl":"10.1016/j.talanta.2025.128377","url":null,"abstract":"Laser-Induced Breakdown Spectroscopy (LIBS) is a widely used technique for elemental analysis. The analysis of the obtained LIBS spectra generally assumes plasma homogeneity. However, using focused laser beams for interrogation, LIBS probes materials on the microscale and is, thus, prone to artefacts from sample heterogeneities on the micrometre scale. An ablation at a material boundary of two matrices may result in a significant inhomogeneity in the plasma plume, which can severely impact the accuracy of quantitative analysis. Since this propagation of the surface morphology into the plasma plume is driven by the plasma expansion, its final impact is strongly pressure dependent. This study examines the influence of varying ambient pressures (7-1000 mbar) on plasma morphology, spectral characteristics, and key plasma properties such as electron number density at a well-defined Cu-Sn boundary, in comparison with the results obtained using homogeneous alloys. Several approaches of plasma imaging with bandpass filters, spectroscopy, and Radon transform-based 3D reconstruction were employed to analyse elemental distribution, signal-to-noise (SNR) and signal-to-background (SBR) ratios, as well as electron number densities. The 3D reconstructions revealed a pronounced plasma asymmetry for the ablation at the material boundary, in contrast to the near-axial symmetry observed for the ablation of homogeneous alloys. At lower pressures, this distinct elemental separation in plasma persisted, while higher pressures led to an increased collisional mixing and homogenization. SNR and SBR were consistently lower for ablation at the boundary compared to homogeneous samples. These findings highlight how boundary ablation contributes to plasma inhomogeneities in LIBS analysis of heterogeneous materials and emphasize the need to account for these effects when using LIBS for elemental mapping of fine heterogeneous structures.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128377"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective and sensitive determination of neopterin in biological fluids using magnetic silica-supported molecularly-imprinted polymers. 用磁性硅支撑的分子印迹聚合物选择性和灵敏地测定生物液体中的新蝶呤。

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1016/j.talanta.2025.128450

Adem Zengin, Necdet Karakoyun, Mustafa Bilici

{"title":"Selective and sensitive determination of neopterin in biological fluids using magnetic silica-supported molecularly-imprinted polymers.","authors":"Adem Zengin, Necdet Karakoyun, Mustafa Bilici","doi":"10.1016/j.talanta.2025.128450","DOIUrl":"10.1016/j.talanta.2025.128450","url":null,"abstract":"Neopterin (NPT) is a key biomarker of immune system activation and holds significant importance in the diagnosis and monitoring of various clinical conditions. In this study, a novel magnetic silica-supported molecularly imprinted polymer (MIP-MS) was synthesized via surface imprinting and integrated with UV-vis spectrophotometry for the selective extraction and quantification of NPT in human serum and artificial cerebrospinal fluid. Comprehensive characterization was carried out and the results confirmed the successful fabrication of the MIP layer with well-defined structural and surface properties. Adsorption kinetics and isotherm studies were best fitted by the pseudo second-order (R2 = 0.9997) and Langmuir model (R2 = 0.9987), respectively and the results revealed the presence of specific recognition sites on the MIP-MS for NPT, resulting in adsorption capacity of 98.3 mg/g and imprinting factor of 4.77. Additionally, the MIP-MS exhibited outstanding reusability, retaining its performance over 10 adsorption-desorption cycles. The developed method demonstrated excellent sensitivity, with a wide linear range (3-300 nM), a detection limit of 1.18 nM and a recovery range of 97.6 %-100.4 %, accompanied by a relative standard deviation below 5.0 %. The findings suggest that the proposed MIP-MS coupled with UV-vis spectrophotometry provides a cost-effective, rapid, and highly selective alternative for NPT detection in biological samples.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128450"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive and visual colorimetric detection of AFB1 based on MOF-on-MOF bimetallic nanozymes. 基于MOF-on-MOF双金属纳米酶的AFB1灵敏和视觉比色检测。

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-05-28 DOI: 10.1016/j.talanta.2025.128404

Yuhang Lin, Ruishuang Yuan, Tianshuo Wang, Yuanhao Liu, Lianxi Pu, Mingxuan Jia, Xilong Zhou, Min Zhang, Lijun Ding, Weiran Zhu, Kun Wang

{"title":"Sensitive and visual colorimetric detection of AFB1 based on MOF-on-MOF bimetallic nanozymes.","authors":"Yuhang Lin, Ruishuang Yuan, Tianshuo Wang, Yuanhao Liu, Lianxi Pu, Mingxuan Jia, Xilong Zhou, Min Zhang, Lijun Ding, Weiran Zhu, Kun Wang","doi":"10.1016/j.talanta.2025.128404","DOIUrl":"10.1016/j.talanta.2025.128404","url":null,"abstract":"Aflatoxin B1 (AFB1) is widely present in food crops and their products, posing significant risks to human health as a contaminant. Therefore, developing reliable, rapid, and convenient detection methods for AFB1 is crucial to ensure food safety. In this study, a MOF-on-MOF strategy was employed to prepare bimetallic MOF, using MIL-101 (Fe) as the host metal-organic framework (MOF) and MIL-125 (Ti), which exhibits excellent peroxidase-like activity and photosensitivity, as the guest. The material was then calcined under a nitrogen atmosphere to promote the carbonization of the framework and the development of a hollow structure, resulting in the fabrication of MIL-125@MIL-101-N2 catalyst. This method utilizes the distinct properties of iron and titanium to lower the reaction barriers in the colorimetric process. The host-guest interaction in the MOF-on-MOF structure prevents the excessive coordination of Fe, which would speed up the recombination of photogenerated electron-hole pairs, thereby broadening the light absorption range and boosting the photogenerated electron capability. Based on the bimetallic MOF-on-MOF strategy, a colorimetric sensor was constructed with MIL-125@MIL-101-N2 material and aptamer as recognition element for detection of AFB1. The results demonstrated a linear relationship between the RGB values and AFB1 concentrations in the range of 1-1380 ng/mL, which can be used for sensitive and visual detection of AFB1.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128404"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single particle and single cell analysis by ICP-MS with pneumatic nebulisation: Comparing sample introduction systems. 气动雾化ICP-MS单颗粒和单细胞分析：样品导入系统的比较。

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-05-22 DOI: 10.1016/j.talanta.2025.128372

Andrés Suárez Priede, Mario Corte-Rodríguez, Hannes Gödde, María Montes Bayón, Jörg Bettmer

{"title":"Single particle and single cell analysis by ICP-MS with pneumatic nebulisation: Comparing sample introduction systems.","authors":"Andrés Suárez Priede, Mario Corte-Rodríguez, Hannes Gödde, María Montes Bayón, Jörg Bettmer","doi":"10.1016/j.talanta.2025.128372","DOIUrl":"10.1016/j.talanta.2025.128372","url":null,"abstract":"Inductively coupled plasma-mass spectrometry (ICP-MS) operated in the single particle (sp) and singe cell (sc) modes belongs to the methods of elemental analysis that has significantly grown during the last years. Its contribution to the characterisation of nanomaterials and cell populations has opened new avenues and possibilities in many different disciplines. In this context, pneumatic nebulisation plays a pivotal role since it is still the preferable sample introduction system in ICP-MS. Reliable quantitative data are only accessible with known transport efficiency (TE) of the small objects, so that the determination of this parameter is extremely critical. In this study, we investigated various combinations of commercially available nebulizers and spray chambers for testing their performance in sp- and sc-ICP-MS. Two configurations made use of high sample flow rates of 0.4 mL/min with a \"traditional\" cyclonic spray chamber, and another four combinations employed spray chambers especially designed for total consumption used at optimum flow rates of 10 μL/min (Cytospray and HE-SIS). Achievable sensitivities were significantly better for the high consumption set-ups (about 5-fold). Transport efficiencies determined by the particle number method were evaluated by the use of three different model suspensions: i) 30 nm standard gold nanoparticles LGCQC5050, ii) europium-loaded polystyrene beads, and iii) selenized yeast SELM-1. Significant differences between the three suspensions were observed obtaining results up to 90 % for certain configurations. In fact, these differences imply that for different approaches (sp- or sc-ICP-MS) a suitable calibrant has to be used for the accurate determination of the transport efficiency.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128372"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of montmorillonite composite alginate beads for micro-solid phase extraction of carbamate residues prior to their determination by ultra performance liquid chromatography-photodiode array detector. 利用超高效液相色谱-光电二极管阵列检测器对氨基甲酸酯残基进行微固相萃取，制备蒙脱土复合海藻酸微球。

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1016/j.talanta.2025.128452

Chutharat Maokam, Pirom Suwannasom, Saksit Chanthai, Onanong Cheerarot, Yanawath Santaladchaiyakit, Jitlada Vichapong

{"title":"Fabrication of montmorillonite composite alginate beads for micro-solid phase extraction of carbamate residues prior to their determination by ultra performance liquid chromatography-photodiode array detector.","authors":"Chutharat Maokam, Pirom Suwannasom, Saksit Chanthai, Onanong Cheerarot, Yanawath Santaladchaiyakit, Jitlada Vichapong","doi":"10.1016/j.talanta.2025.128452","DOIUrl":"10.1016/j.talanta.2025.128452","url":null,"abstract":"This paper reports the employment of natural montmorillonite (Cloisite Na+) and sodium alginate (Alg) to fabricate sorbent beads and applied for extraction and preconcentration of carbamates (CBMs) based on micro-solid phase extraction (μ-SPE) method prior to analysis by ultraperformance liquid chromatography coupled with a photodiode array detector (UPLC-PDA). Cloisite Na+/Alg composite bead was synthesized by ionic gelation method using calcium chloride as a cross-linking agent at ambient temperature. The biocomposite sorbent bead was then characterized by various techniques, including scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The parameters affected the extraction efficiency were investigated and optimized. Based on the optimal extraction conditions, the method provided linearity in the range of 1.0-1000 μg L-1, with determination coefficient greater than 0.99. The limits of detection ranged between 0.7 and 10 μ g L-1 for all analytes. The relative standard deviation was less than 4.6 % and 6.2 % for intra-day and inter-days, respectively. Finally, the μ-SPE-UPLC-PDA method was successfully used to CBMs residue detection in food and environment samples. The percentage recoveries were found in the range of 70-117 %. Moreover, the result in real sample found that no residue of the studied carbamates was observed in sample studies. The application of greenness and blueness assessment tools (AES, AGREE, and BAGI) confirmed that μ-SPE can be considered a green chemistry method. The proposed investigated method offers advantages such as low cost, simplicity, reduced solvent consumption, and minimal environmental impact, making it a promising alternative to conventional methods for carbamate pesticides analysis.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128452"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of geochemical and mineral compositions of volcanic soil affected by Merapi eruption in Central Java Indonesia using laser-induced breakdown spectroscopy with calibration-free. 用激光诱导击穿光谱法分析印尼中爪哇受默拉皮火山喷发影响的火山土壤的地球化学和矿物组成

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-05-27 DOI: 10.1016/j.talanta.2025.128376

Ali Khumaeni, Rina Dwi Indriana, Felix Jonathan, Dian Fiantis, Frisa Irawan Ginting, Nasrullah Idris, Hendrik Kurniawan

{"title":"Analysis of geochemical and mineral compositions of volcanic soil affected by Merapi eruption in Central Java Indonesia using laser-induced breakdown spectroscopy with calibration-free.","authors":"Ali Khumaeni, Rina Dwi Indriana, Felix Jonathan, Dian Fiantis, Frisa Irawan Ginting, Nasrullah Idris, Hendrik Kurniawan","doi":"10.1016/j.talanta.2025.128376","DOIUrl":"10.1016/j.talanta.2025.128376","url":null,"abstract":"Volcanic materials are crucial for the rejuvenation process as they positively influence agricultural factors by progressively enhancing soil fertility. In this study, the quantification of geochemical and mineral constituents of Merapi volcanic soil was carried out to assess its substantial influence on enhancing soil fertility using laser-induced breakdown spectroscopy (LIBS) technique via a calibration-free approach. The volcanic soil samples were obtained from 24 locations in the Merapi volcano in Central Java Indonesia. In the experiment, a pulse Nd:YAG laser (1064 nm, 83 mJ, 10 ns) was experimentally directed and focussed on the samples to generate a luminous plasma containing the elemental makeup of the samples. The volcanic soil in all directions contained the same elements with different concentrations for each sampling location. The volcanic soil contains major elements of Si, Ca, Fe, Al, and K and minor elements of P, Ti, Mn, Cu, and Zn. The soil of Merapi volcano contains a total of 13 oxides, including three elemental oxides from the silicate mineral framework (SiO2, Al2O3, Fe2O3), five essential macronutrients for plant growth (CaO, MgO, K2O, SO3, P2O5), five micronutrients for plant growth (MnO, CuO, ZnO, Cl, Br), and two total oxide elements from rare earth elements (Y2O3 and Eu2O3). The results of the LIBS method demonstrate a substantial concordance with the findings obtained from the conventional X-ray fluorescence (XRF) technique.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128376"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polarity-switchable photoelectrochemical detection of tyrosinase activity based on enzyme-triggered boronic ester bridging between In₂S₃ and Cu₂ZnSnS₄. 基于In2S3和Cu2ZnSnS4之间硼酯桥接的极性可切换光电化学检测酪氨酸酶活性。

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-05-27 DOI: 10.1016/j.talanta.2025.128405

Rong Liao, Bowen Yan, Shihua Wang, Wenfang Deng, Yueming Tan, Qingji Xie

{"title":"Polarity-switchable photoelectrochemical detection of tyrosinase activity based on enzyme-triggered boronic ester bridging between In2S3 and Cu2ZnSnS4.","authors":"Rong Liao, Bowen Yan, Shihua Wang, Wenfang Deng, Yueming Tan, Qingji Xie","doi":"10.1016/j.talanta.2025.128405","DOIUrl":"10.1016/j.talanta.2025.128405","url":null,"abstract":"Herein, an innovative polarity switchable strategy for photoelectrochemical (PEC) detection is reported by utilizing enzyme-triggered boronic ester bridging between two semiconductors. Monophenol-modified In2S3 is prepared by sequentially coating polyethyleneimine (PEI) and covalently conjugating 4-hydroxyphenylacetic acid (PhOH) on In2S3, whereas dihydroxy-modified Cu2ZnSnS4 is prepared through surface coating of chitosan (CS). Under the catalysis of tyrosinase (TYR), monophenol groups of In2S3@PEI-PhOH undergo the conversion to o-diphenol groups. With the aid of 1,4-phenylboronic acid, boronate ester bridges form between TYR-treated In2S3@PEI-PhOH and Cu2ZnSnS4@CS, so In2S3 can be anchored on the Cu2ZnSnS4@CS-modified electrode surface. As a result, the formation of p-n heterojunctions on the photoelectrode can reverse the photocurrent polarity, enabling polarity switchable PEC detection of TYR activity. The linear range is from 0.01 to 5 U mL-1, and the detection limit is 0.0015 U mL-1 for TYR activity detection. The established method is successfully employed for TYR activity detection in human serum samples.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128405"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple one-pot assay for visual and smartphone-based quantification of human albumin in urine and biological fluids. 简单的一锅试验，用于视觉和智能手机定量人类尿液和生物液体中的白蛋白。

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-06-07 DOI: 10.1016/j.talanta.2025.128443

Nopchulee Cheeveewattanagul, Oranut Warachit, Paskorn Muangphrom, Patsamon Rijiravanich

{"title":"Simple one-pot assay for visual and smartphone-based quantification of human albumin in urine and biological fluids.","authors":"Nopchulee Cheeveewattanagul, Oranut Warachit, Paskorn Muangphrom, Patsamon Rijiravanich","doi":"10.1016/j.talanta.2025.128443","DOIUrl":"10.1016/j.talanta.2025.128443","url":null,"abstract":"This study introduces a simple and effective one-pot assay for the quantification of human albumin (HA) using H8 aptamer-modified magnetic beads (H8Apt-MB-HA), applicable across various biological fluids, including urine, serum, and sweat. Upon binding to HA, the aptamer-bead complexes exhibit concentration-dependent aggregation: well-dispersed at low HA levels and clustered at higher concentrations, with rapid sedimentation under a magnetic field in U-shaped well plates. These aggregation patterns are visually discernible, enabling rapid qualitative screening without the need for complex instrumentation. For quantitative analysis, digital images captured by a smartphone or a plate scanner (Cytation™ 5) are processed using ImageJ software, allowing for accurate signal quantification. The top and bottom-view assay achieves a detection limit of 5.9 and 6.5 mg L-1 and demonstrates robust performance across a urinary pH range of 5.0-7.0. Minimal cross-reactivity with other biological components confirms the aptamer's high specificity. Clinical validation with patient urine samples shows excellent correlation with standard immunoturbidimetric methods, underscoring the assay's potential for point-of-care testing. This approach supports immediate interpretation and clinical decision making, especially valuable in resource-limited settings and for routine monitoring of kidney-related disorders.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128443"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel pH-responsive aptamer-molecularly imprinted hydrogel for efficient identification and separation of ampicillin in complex matrices. 一种新型ph响应适体-分子印迹水凝胶，用于复杂基质中氨苄西林的有效鉴定和分离。

IF 6.1 1区化学

Talanta Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1016/j.talanta.2025.128425

Liyu Qiu, Xixiang Yang, Nan Zhao, Anqi Luo, Li Wang, Xiaogang Hu

{"title":"A novel pH-responsive aptamer-molecularly imprinted hydrogel for efficient identification and separation of ampicillin in complex matrices.","authors":"Liyu Qiu, Xixiang Yang, Nan Zhao, Anqi Luo, Li Wang, Xiaogang Hu","doi":"10.1016/j.talanta.2025.128425","DOIUrl":"10.1016/j.talanta.2025.128425","url":null,"abstract":"Hybrids of molecular imprinting-aptamers have been used for environmental analysis; however, the weak selectivity and stability inhibited their applications in complex matrices. In this paper, the nano-scale pH-responsive aptamer-molecularly imprinted interpenetrating hydrogels (Fe3O4@Apt-MIH) were prepared in one step by the modified sol-gel method based on the preparation principle of interpenetrating network hydrogels, with Fe3O4 as the carrier, aminopropyltriethoxysilane and methyltrimethoxysilane as functional monomers, polyethyleneimine (PEI) as the pH-responsive components, and tetraethoxysilane (TEOS) and glutaraldehyde (GA) as dual cross-linking agents. After optimizing the polymerization and extraction conditions, the pH response performance, extraction capacity and reusability of the material with highly magnetism were investigated, which could achieve magnetic separation within 5 s. The SiO2 coating hydrolyzed by TEOS provided a rigid backbone for interpenetrating hydrogels, which could improve the acid resistance of PEI by adjusting the isoelectric point of Fe3O4@Apt-MIH and assist in target identification. Selectivity research showed that aptamers and functional monomers have a highly effective synergistic recognition effect on ampicillin, with the imprinting factor of 6.90 and the synergistic recognition factor of 1.48. This solid-phase microextraction material coupled to high-performance liquid chromatography with the linear range of 10-1000 μg/L and the detection limit of 7.67 μg/L enabled the selective separation and detection of ampicillin in milk and honey, with minimal sample matrix interference and recovery between 95.2 and 102 %.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128425"},"PeriodicalIF":6.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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