Talanta最新文献

{"title":"An AIE fluorescence probe for ratiometric and selective detection of Sn²⁺ based on proton transfer.","authors":"Duo-Duo Hu, Guangnan Wei, Ping Yang, Dexin Fu, Kuiliang Li","doi":"10.1016/j.talanta.2025.128078","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128078","url":null,"abstract":"<p><p>Detecting Sn²⁺ heavy metal ions is crucial for mitigating water pollution and safeguarding human health. This study involved the design and synthesis of a novel fluorescence probe, RTPE-IM, which is a combination of an aggregation-induced emission luminophore (TPE) and rhodamine B. The probe undergoes proton transfer in the presence of Sn²⁺ ion, displaying high selectivity and a low detection limit of 0.121 μM. The synthesis and application of this molecule offer a potential strategy for preventing environmental health risks.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128078"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode sensor innovation based on light-harvesting and nanozyme integrated Cu-MOF loaded Cu₂O label.","authors":"Man Xu, Zheng Wang, Yingzhuo Shen, Meijuan Zhao, Xiaoya Hu, Hongbo Li, Wei Liu, Qin Xu","doi":"10.1016/j.talanta.2025.128012","DOIUrl":"10.1016/j.talanta.2025.128012","url":null,"abstract":"<p><p>Integrating photoelectrochemical (PEC) and colorimetric dual-mode shed light on \"all-in-one\" sensing platform for high sensitivity, in situ visualization and self-validating measurement. Its attainment depends on the capabilities of light harvesting and colorimetric signal generation from bi-functional tags, which remains challenging. Herein, Cu-MOF loaded Cu₂O (Cu₂O@Cu-MOF) was deliberately prepared and employed as the PEC- colorimetric signal reporter with tail-made stable photo-current and peroxidase mimic activity. A thin layer of Zr-MOF was deliberately encapsulated onto Cu₂O@Cu-MOF for the immobilization of aptamer (Apt) which can be captured by the magnetic AuNPs/Fe₃O₄ nanoparticles through duplex-specific hybrid. In the presence of target analyte, PEC- colorimetric signal reporter was quantitatively released from the magnetic AuNPs/Fe₃O₄ after one-step convenient magnetic separation, facilitating photocurrent response by the photo-induced excitation and colorimetric response by catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). In a proof-of-concept experiment, a customized PEC- colorimetric dual-mode sensor platform based on Cu₂O@Cu-MOF reporter has achieved an ultrasensitive response for bisphenol A (BPA), exhibiting sub-picomole (PEC signal) and sub-nanomole (colorimetric signal) detection limits, respectively. Our dual-mode sensor platform strategy aims to extend to high-sensitivity and visual analysis in fields of in vitro diagnosis, environmental monitoring, and food safety.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"128012"},"PeriodicalIF":5.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of plastic blends based on virtual mid-infrared spectroscopy combined with chemometric methods.","authors":"Jian Yang, Yu-Peng Xu, Xiao-Li Chu","doi":"10.1016/j.talanta.2025.128006","DOIUrl":"10.1016/j.talanta.2025.128006","url":null,"abstract":"<p><p>Developing efficient and accurate quantitative analysis methods for plastic blends holds significant value for resource recycling and environmental monitoring. Mid-infrared (MIR) spectroscopy, combined with chemometric techniques, has demonstrated excellent performance in plastic blend quantification. However, obtaining mid-infrared spectral data for a large number of plastic blends to calibrate the model remains challenging. This study proposes an innovative approach that utilizes pure plastic MIR spectra and the Beer-Lambert law to generate virtual plastic blend spectra. Four experimental groups (A-D) were designed, incorporating both real and virtual spectra to systematically evaluate the method's effectiveness. Experiment group A established a baseline model using real spectra, while experiment groups B, C, and D respectively validated the applicability and generalization capability of models based on virtual spectra, as well as their potential applications in MIR hyperspectral imaging (MIR-HSI), respectively. The study further explores feature band selection, model construction, evaluation, and interpretation. The results demonstrate that this method can efficiently predict the mass percentages of components in ternary plastic blends. In experimental group C, partial least squares regression (PLSR), one-dimensional convolutional neural network (CNN1D), and two-dimensional convolutional neural network based on Gramian Angular Field (GAF-CNN2D) models-trained on 208 virtual plastic blend spectra-were employed to predict the mass percentages of 66 ternary plastic blends composed of polyethylene (PE), polypropylene (PP), and polystyrene (PS). The prediction coefficients of determination (R_T²) reached 0.9872, 0.9879, and 0.9944, respectively, indicating exceptional predictive accuracy. Experimental group D further demonstrated that, even under Gaussian noise interference and limited spectral range, the strategy of fusing mid-wave infrared and long-wave infrared bands allowed the PLSR and GAF-CNN2D models to maintain high performance in predicting the mass percentages of 66 ternary blends of PE, PP, and PS, with R_T² values of 0.9852 and 0.9895, respectively. This suggests that the proposed method holds potential for applications in MIR-HSI and is promising for real-time online analysis. Finally, this study proposes a more widely applicable and optimized quantitative analysis application scheme based on virtual plastic blend spectra, aiming to enable rapid and precise determination of unknown plastic blends.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"128006"},"PeriodicalIF":5.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imidazole-triggered in situ fluorescence reaction system for quantitatively determination of dopamine from multiple sources.","authors":"Yifei Ma, Lijie Zhang, Hong Yang, Shanshan Zhu, Jinhua Liu","doi":"10.1016/j.talanta.2025.127975","DOIUrl":"10.1016/j.talanta.2025.127975","url":null,"abstract":"<p><p>Highly selective and sensitive determination of dopamine (DA) from multiple sources remains a persistent and significant challenge. Here, we develop an imidazole-triggered in situ fluorescence reaction system for highly selective and sensitive determination of DA from various sources (human, horse, dog, rabbit, and mouse). The system operates by catalyzing the oxidation of DA with 1,5-Dihydroxynaphthalene (1,5-DHA) through a Lewis base formed by imidazole, leading to the rapid generation of yellow azamonardine fluorescent compounds (AFC). Notably, the system demonstrates minimal background noise and a high signal-to-noise ratio of up to 300-fold with a determination limit of 33.33 pM, making it 10-100 times more sensitive than conventional enzyme-linked immunosorbent assay (ELISA) methods. Moreover, selectivity tests reveal that our system can effectively distinguish between several common interfering substances, even at concentrations as low as 10 nM. The developed system shows promising results in detecting DA from diverse sources (humans, horses, dogs, rabbits, and mice), including urine samples from clinical patients, exhibiting good agreement with traditional ELISA kits. Therefore, the established in situ fluorescence reaction system holds great potential for the determination of DA-related disorders due to its impressive analytical capabilities.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"127975"},"PeriodicalIF":5.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring variations in mitochondrial hydrogen sulfide using two-photon cyclometalated iridium(III) complex probe: A new strategy for ischemia-reperfusion drug discovery and efficacy evaluation.","authors":"Peng Du, Tianao Zhang, Ying Wu, Zongping Yu, Chaolong Liu","doi":"10.1016/j.talanta.2025.128021","DOIUrl":"10.1016/j.talanta.2025.128021","url":null,"abstract":"<p><p>Hepatic ischemia-reperfusion injury (HIRI) is one of the main causes of liver insufficiency and failure after liver surgery. However, the effectiveness of current methods of treating HIRI is generally limited. Previous studies have shown that hydrogen sulfide (H₂S) has a beneficial effect on HIRI, and an appropriate concentration of H₂S can significantly reduce HIRI by protecting the mitochondria. Therefore, establishing an accurate imaging platform for monitoring variations in mitochondrial H₂S is an effective strategy for anti-HIRI drug discovery and efficacy evaluation. To this end, a cyclometalated iridium(III) complex-based probe, Cym-Ir-EDB, was developed for detecting mitochondrial H₂S in HIRI. Cym-Ir-EDB possesses good sensitivity, high selectivity, negligible cytotoxicity, and excellent mitochondrial-targeting ability, rendering it a promising imaging tool for analyzing variations in mitochondrial H₂S in HIRI cells. Using Cym-Ir-EDB as a probe, anti-HIRI drugs were screened from isothiocyanates by monitoring variations in mitochondrial H₂S in HIRI cells, for the first time. Moreover, the dynamics of mitochondrial H₂S in HIRI cells were visualized and the response of HIRI to treatment with the screened erucin was monitored. The findings indicate that Cym-Ir-EDB can serve as a useful imaging platform for the precise imaging of mitochondrial H₂S in HIRI, thereby contributing to anti-HIRI drug discovery and efficacy evaluation.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"128021"},"PeriodicalIF":5.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple CRISPR zones-driven ultrasensitive detection of DNA via CRISPR-Cas12a and ligation-rolling circle amplification","authors":"Guigao Lin ,&nbsp;Jing Li ,&nbsp;Kuo Zhang","doi":"10.1016/j.talanta.2025.128336","DOIUrl":"10.1016/j.talanta.2025.128336","url":null,"abstract":"<div><div>The ability to detect specific DNA, including single nucleotide variants (SNVs), with high sensitivity is essential for advancing genetic research, diagnostics, and personalized medicine. This study presents a novel method for ultrasensitive DNA detection, combining ligation-rolling circle amplification (L-RCA) with CRISPR-Cas12a. While L-RCA systems have been widely used for nucleic acid detection, the sensitivity of conventional L-RCA generally reaches approximately 100 pM. Here, we demonstrate that the sensitivity of RCA-Cas12a systems can be markedly enhanced by incorporating multiple CRISPR target regions into the padlock probe. This method achieves remarkable sensitivity, detecting DNA at concentrations as low as 1 aM (6 copies per reaction), and is capable of identifying single nucleotide variants (SNVs) with allele fractions as low as 1 %. Unlike many current complex RCA-Cas12a strategies, this approach is simple and does not require advanced labeling or instrumentation, making it a promising tool for ultrasensitive DNA detection in various applications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"Article 128336"},"PeriodicalIF":5.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted LC-MS/MS profiling and semi-quantification of Gly-Pro-Yaa and Gly-Hyp-Yaa tripeptides in collagen hydrolysates","authors":"Mingkai Bai ,&nbsp;Mengyao Ma ,&nbsp;Zexin Meng ,&nbsp;Jianwei Yu ,&nbsp;Meichao Zhang ,&nbsp;Wenhai She ,&nbsp;Lerong Zhao ,&nbsp;Suqin Zhu ,&nbsp;Zifang Zhao ,&nbsp;Adewale Olusegun Obadina ,&nbsp;Mingyong Zeng ,&nbsp;Shiyang Li ,&nbsp;Haohao Wu","doi":"10.1016/j.talanta.2025.128317","DOIUrl":"10.1016/j.talanta.2025.128317","url":null,"abstract":"<div><div>Gly-Pro-Yaa and Gly-Hyp-Yaa are characteristic triplet sequences in collagens, and tripeptides of both types are of interest for featuring proline hydroxylation sites and quality evaluation of commercial peptide products. Current LC-MS/MS multiple reaction monitoring (MRM) methods could only detect selected Gly-Xaa-Yaa tripeptides in collagen hydrolysates owing to the limit of MRM channels. Here, an approach for simultaneous screening and semi-quantifying Gly-Pro-Yaa and Gly-Hyp-Yaa tripeptides was developed based on LC-MS/MS precursor ion scan, which provides broader analyte coverage compared to MRM's target-restricted detection. The diagnostic fragment ions at <em>m</em>/<em>z</em> 127 and 143, as well as the precursor <em>m</em>/<em>z</em> ranges of 230–336 and 246−252, were effective for detecting Gly-Pro-Yaa and Gly-Hyp-Yaa tripeptides, respectively, and there were 8 Gly-Pro-Yaa tripeptides and 12 Gly-Hyp-Yaa tripeptides screened out from 25 commercial collagen tripeptide products. By calculating the relative response factors (0.59−1.7) of six candidates, Gly-Pro-Hyp and Gly-Hyp-Ala were chosen as the preferred calibration standards for semi-quantification of individual/total Gly-Pro-Yaa and Gly-Hyp-Yaa tripeptides, respectively, with sensitivity, linearity and reproducibility validated. Gly-Pro-Hyp, Gly-Pro-Ala, Gly-Pro-Arg, Gly-Hyp-Arg, Gly-Hyp-Ala, and Gly-Hyp-Lys were abundantly found with over 70 % of the commercial products containing 1 %–9 % of any one of them. Our study provides a novel LC-MS/MS method for the characterization of X-position proline hydroxylation in collagens and for the comprehensive profiling and semi-quantification of characteristic tripeptides in commercial collagen tripeptide products.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"Article 128317"},"PeriodicalIF":5.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial identification in SERS-integrated microfluidics using CNN-driven 2D classification of 1D spectra","authors":"Mehdi Feizpour ,&nbsp;Halewijn Van den Bosche ,&nbsp;Lilit Melikyan ,&nbsp;Thomas Demuyser ,&nbsp;Piet Cools ,&nbsp;Hugo Thienpont ,&nbsp;Tatevik Sarukhanyan ,&nbsp;Heidi Ottevaere","doi":"10.1016/j.talanta.2025.128325","DOIUrl":"10.1016/j.talanta.2025.128325","url":null,"abstract":"<div><div>Bacterial sensing involves complex and variable samples that require advanced handling and analytical methods. To address these challenges, machine learning—especially deep learning—and SERS-based microfluidics have shown great promise. While previous studies have majorly focused on 1D spectral classification, the use of 2D representations of SERS spectra has not yet been explored, particularly for on-chip bacterial identification. In this work, we introduce a novel framework that combines SERS-enabled microfluidics with optimized 2D convolutional neural networks (2D-CNNs) for bacterial classification. SERS integration inside microfluidic chips was achieved through direct laser writing, enabling custom active areas and efficient on-chip measurements. We systematically evaluated nine distinct 1D-to-2D spectral transformations, with spectrogram and continuous wavelet transform yielding test accuracies of 99 % and 97 %, respectively, on controlled datasets. Using transfer learning, we achieved 100 % accuracy on the on-chip dataset, demonstrating the model's adaptability to new data. In contrast, other transformations, like pairwise distance and autocorrelation, performed below 93 %, indicating their limited ability to capture subtle spectral features. This framework offers high sample control, parallelization, and the potential for expanding the bacteria database, making it ideal for low-data-volume situations such as rare infections. Further development and testing across strains, environments, and practical challenges can further improve our approach's reliability for real-world diagnostics.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"Article 128325"},"PeriodicalIF":5.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fully printed field-effect transistor humidity sensor with chitosan/polyvinyl alcohol/nano carbon powder for enhanced moisture sensitivity.","authors":"Ruifang Liu, Zhenting Wu, Qiang Li, Sara Shamim, Long Ba","doi":"10.1016/j.talanta.2025.127679","DOIUrl":"10.1016/j.talanta.2025.127679","url":null,"abstract":"<p><p>With the advancement of flexible electronics, the demand for low-cost, high-performance flexible humidity sensors for wearable devices has increased significantly. However, commercial humidity sensors require complex preparation methods and are expensive. Therefore, we report polyvinyl alcohol/chitosan/nano carbon powder (PVA/CS/NCP) humidity-sensitive composite materials, which were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and contact angle measurements. A resistive humidity sensor was fabricated using screen printing, and the device showed a good response at 33%RH-98%RH humidity, and at 98 % RH, the sensor achieved a response of 1.74. A fully printed field-effect transistor (FET) humidity sensor was prepared by integrating an ion gel transistor with a CS/PVA/NCP humidity-sensitive resistor to improve the response. The comparison reveals a significant improvement in the FET humidity sensor's response, reaching 6.27 at 98 % RH. In addition, both types of sensors exhibit less hysteresis and good repeatability. Lastly, we tested the constructed humidity sensors under non-contact and variable breathing conditions, laying the groundwork for future wearable applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127679"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a porous zirconium-based MOF nanoplate as an affinity ECL platform for the detection of protein kinase activity and inhibitor screening.","authors":"Sasan Radfar, Milad Sheikh, Amirreza Akhavantabib, Amirhossein Heidari, Milad Ghasemi, Mohammadreza Naghavi, Reza Ghanbari, Farkhonde Zibadi, Babak Jamshidi, Abdolhamid Alizadeh","doi":"10.1016/j.talanta.2025.127675","DOIUrl":"10.1016/j.talanta.2025.127675","url":null,"abstract":"<p><p>Abnormal kinase expression affects phosphorylation in the human body, which is associated with numerous diseases, including cancer, diabetes mellitus, and Alzheimer's disease. In this study, we synthesized a highly stable, two-dimensional, luminescence-functionalized metal-organic framework with remarkable electrochemiluminescence (ECL) by immobilizing 9,10-Di(p-carboxyphenyl) anthracene (dca) on a zirconium cluster (dca-Zr₁₂) via a strong coordination bond between -COO⁻ and Zr⁴⁺. This novel and simple platform relies on the highly specific identification of phosphate molecules by the ultra-thin dca-Zr₁₂ nanoplate through carboxylate-Zr⁴⁺-phosphate chemistry. The ferrocene-labeled peptide substrate (Fc-S-Peptide) was phosphorylated in the presence of protein kinase A (PKA) and adenosine 5'-triphosphate (ATP), and the resulting phosphopeptide could subsequently be precisely captured by the zirconium sites of the dca-Zr₁₂-modified electrode and, eventually, quench the ECL and gain a signal-off state. This rapid and simple detection strategy was successfully employed to measure PKA activity, with a detection limit as low as 0.35 mU mL^-1. Based on the results, it exhibited high selectivity and can be applied for screening PKA inhibitors. The technique was subsequently applied to detect protein kinase activity in drug-stimulated MCF-7 cell lysates, demonstrating its potential for kinase-related investigations. Further, this platform could identify the activity of other kinase types with universal applicability.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127675"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}