Potential-resolved electrochemiluminescence multiplex immunoassays with isolated cathodic and anodic co-reactants and pre-oxidized Ag-doping methionine-stabilized Au nanoclusters.
Qiao Zhang, Ruoqi Wang, Lei Yu, Yunwei Gao, Dazhong Shen
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Abstract
In this work, a potential-resolved electrochemiluminescence (ECL) multiplex immunoassay (MIA) was developed using Ag-doping methionine-stabilized Au nanoclusters (Met-AuAgNCs) with immobilized co-reactant as the anodic ECL tag and nanocomposite of gold nanoparticles/graphene oxide/N, N'-dicaproate sodium-3,4,9,10-perylene-dicarboximide (AuNPs/GO/PDI) as the cathodic ECL tag. Compared with methionine-stabilized Au nanoclusters (Met-AuNCs), the ECL of Met-AuAgNCs was enhanced 5.61-fold. When anodic co-reactant of N,N-diethylethylenediamine (DEDA) was connected to Met-AuAgNCs, the ECL of DEDA-Met-AuAgNCs was 11.3-fold of that of Met-AuAgNCs in DEDA solution due to the shorter charge transfer distance between Met-AuAgNCs and DEDA. After a pre-oxidation at 0.95 V for 60 s, the ECL of DEDA-Met-AuAgNCs was further enhanced by 10.6- and 27.9-fold in the cyclic voltammetric and potential step modes, respectively. The pre-oxidation ECL enhancement was demonstrated by an immobilized co-reactant promoters mechanism. In a potential-resolved ECL-MIA, carbohydrate antigen 125 and carbohydrate antigen 19-9 were adopted as model analytes, with the detection limits of 0.029 and 0.076 mU mL-1, respectively. The work provides a proof of concept using self-ECL luminophores with immobilized co-reactant promoters in situ formed for potential-resolved ECL-MIAs with isolated anodic and cathodic co-reactants.
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Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
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