Lijun Mao, Wenhua Bi, Jianqi Ye, Xinhao Wan, Yarui Tang, Yao Chen, Wei Liu, Dan Wen
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引用次数: 0
Abstract
The development of highly active nanozymes for advanced bioanalysis has attracted considerable attention, yet significant challenges remain. Inspired by the structural features of natural oxidases with transition metal active sites, we designed a bimetallic CuFe aerogel with significantly enhanced oxidase-like activity for the facile and visual detection of ascorbic acid (AA). The incorporation of Fe into the Cu aerogel matrix resulted in a remarkable 7.8-fold increase in oxidase-like activity compared to pure Cu aerogel, as confirmed by comprehensive experimental analyses and theoretical calculations. Leveraging the inhibitory effect of AA on the Cu6Fe aerogel-catalyzed oxidation reaction, we developed a rapid and sensitive colorimetric detection method, achieving a low detection limit of 0.1 μM. Furthermore, by integrating the Cu6Fe aerogel with portable test strips and smartphone-based colorimetric analysis, we constructed a visual sensing platform capable of accurate and reliable AA quantification. The practical applicability of this platform was successfully demonstrated through the detection of AA in real-world samples, including fruits and commercial vitamin C beverages. This work not only provides a novel strategy for designing highly active metal aerogel-based nanozymes but also opens new avenues for their application in visual sensing and point-of-care diagnostics.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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