{"title":"用于快速检测293T细胞裂解液中p53蛋白的级联锥形七芯纤维生物传感器。","authors":"Zhichao Zhang, Zefeng Li, Ruen Xie, Zhen Tian, Yicun Yao, Bingwu Yang, Jingao Zhang, Haili Ma, Lan Rao, Kuiru Wang, Binbin Yan, Xinzhu Sang, Chongxiu Yu, Bo Fu, Shengli Pu, Jinhui Yuan, Qiang Wu","doi":"10.1016/j.talanta.2025.128384","DOIUrl":null,"url":null,"abstract":"<p><p>P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05-5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"128384"},"PeriodicalIF":6.1000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate.\",\"authors\":\"Zhichao Zhang, Zefeng Li, Ruen Xie, Zhen Tian, Yicun Yao, Bingwu Yang, Jingao Zhang, Haili Ma, Lan Rao, Kuiru Wang, Binbin Yan, Xinzhu Sang, Chongxiu Yu, Bo Fu, Shengli Pu, Jinhui Yuan, Qiang Wu\",\"doi\":\"10.1016/j.talanta.2025.128384\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05-5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.</p>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":\"295 \",\"pages\":\"128384\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.talanta.2025.128384\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/25 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.talanta.2025.128384","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/25 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate.
P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05-5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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