Bin Yang, Jiao Lu, Zhou Wu, Yong Li, Jun Wang, Ruihong Yao, Yanli Li, Zhihao Lu, Zhirong Geng, Zhilin Wang
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引用次数: 0
Abstract
Adenosine 5'-triphosphate (ATP) is a high-energy phosphate compound that provides most of the energy needed for the physiological activities of organisms. The abnormal fluctuation of intracellular ATP level is closely related to a variety of physiological diseases. So, the monitoring of ATP levels is of great significance for the diagnosis and treatment of diseases. Herein, tetraphenylethylene and triphenylamine fluorophores were employed to synthesize six fluorescent probes with different structures, including TPE-P1, TPE-P2, TPE-P3, TPA-P4, TPA-P5 and TPA-P6. These probes all exhibited the advantages of long-wavelength emission, good biocompatibility and large Stokes shifts. Notably, the nitrogen-containing positively charged site and boric acid group of these probes could be used as ATP recognition groups. Then, the selectivity experiments indicated that TPA-P4 possessed good selectivity and higher fluorescence enhancement degree for ATP compared with the other probes, and was more suitable for detection of ATP. Further, TPA-P4 was successfully applied to the imaging detection of ATP in mitochondria. Importantly, TPA-P4 could also distinguish between normal liver cells (LO2) and liver cancer cells (HepG2) by imaging ATP, which may show great potential applications in tumor diagnosis. It had also been successfully applied to the detection of ATP levels during liver injury and inflammation, which is expected to provide important information for the diagnosis and treatment of diseases in the future.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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