Analytica Chimica Acta最新文献

{"title":"Polypeptide functionalized gold nanoprobes for SERS-fluorescence detection and imaging of caspase-9 during apoptosis","authors":"Xiaoyuan Ma, Yue Pan, Xichi Lin, Zhouping Wang","doi":"10.1016/j.aca.2024.343458","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343458","url":null,"abstract":"<h3>Background</h3>Deoxynivalenol is one of the common fungal toxins in processed grain foods. It has the characteristic of high temperature resistance. Dietary intake of DON contaminated food can cause adverse symptoms. Its cytotoxicity is mainly associated with the expression of apoptosis and interfering with protein synthesis. Among which, caspase family proteases play a crucial role in different types of apoptosis signaling pathways. Thus, it is important to develop a platform for real-time and in situ monitoring of caspase in living cells.<h3>Results</h3>In this paper, a polypeptide functionalized gold nanoprobes was designed for real-time and in situ detection of caspase-9 in living cells during DON induced apoptosis. Highly anisotropic gold nanostars (AuNSs) with good LSPR effect were synthesized. It could either serve as the surface enhanced Raman scattering (SERS) substrate or quench fluorescence through fluorescence resonance energy transfer (FRET). Polypeptide containing the LEHD (Leu-Glu-His-Asp) sequence was connected to AuNSs through Au-S bonds. During DON induced cell apoptosis, caspase-9 was activated, which could specifically cleave the recognition site LEHD, causing the polypeptide chain modified with Rhodamine B (Rb) signal group to fall off and move away from AuNSs, ultimately reducing the SERS signal and enhancing the fluorescence signal in the system. The experimental results showed that the nanoprobe had high sensitivity, with a linear range of 5 ng/mL to 400 ng/mL and a minimum detection limit of 0.38 ng/mL.<h3>Significance</h3>This method achieved dual signal quantification and visualization imaging of fluorescence and SERS for caspase-9. The application of nanomaterials has been broadened and the assay was well versatile in different human cell lines. It provided a new platform in studying the relationship between food safety and cellular homeostasis mechanisms.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Host Cell Proteins Profiling in Biopharmaceuticals by A Sensitivity Enhanced Mass Spectrometry Strategy Using TMT-labeling and Signal Boosting","authors":"Andong Xue, Linlin Kong, Jialin Li, Yuxin Jiao, Zhishang Hu, Bin Fu, Guibin Wang, Wanjun Zhang, Jianheng Li, Weijie Qin","doi":"10.1016/j.aca.2024.343445","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343445","url":null,"abstract":"<h3>Background</h3>Host Cell Proteins (HCPs) are impurities expressed in host cells during the biopharmaceutical production process, which. may compromise product quality and potentially leading to immunogenic reactions or other adverse effects. Mass spectrometry (MS)-based strategy is more and more considered as a promising method for HCPs analysis, since it is capable of simultaneously quantifying thousands of proteins in a single test. However, considering the large excess biopharmaceutical product protein present in the system and the extremely low abundance of HCPs, sensitive MS methods are urgently needed in HCPs analysis.<h3>Results</h3>In this work, we introduced a novel approach that leveraged host cell lysate as a boosting channel to enhance the MS signal of the residue HCPs in biopharmaceutical products using isobaric TMT labeling, thereby elevating the low-abundant HCPs to detectable and quantifiable levels of current MS without using enrichment or depletion method to avoid disturbance of the original concentration of the HCPs. Our method surpassed previous benchmarks by identifying a significantly higher number (23844 unique peptides for 3475 proteins) compared to existing records (4541 unique peptides for 848 proteins) for HCPs analysis in RM8671 NIST monoclonal antibody (mAb), demonstrating unparalleled sensitivity and robustness. Furthermore, our workflow successfully identified 44 of 48 UPS1 proteins across a concentration range of 0.32 to 4.15 ppm in monoclonal antibodies (mAbs), proving its effectiveness for in-depth HCPs analysis in biopharmaceuticals.<h3>Significance</h3>Present even at sub-ppm levels, HCPs may compromise the stability and safety of product proteins and alter pharmacokinetics or neutralization of therapeutic effects. Our MS signal enhancing method presented an advancement in HCP analysis, combining improved sensitivity and increased scale of HCPs with a streamlined and robust workflow. This method allowed HCPs quantification at &lt;1 ppm level without disturbance of the original HCPs concentration, which is still rare in the field.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"18 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of biogenic selenium nanoparticles in hypersaline media by single particle inductively coupled plasma mass spectrometry: Haloferax mediterranei case.","authors":"Nuria Guijarro-Ramírez, Iraide Sáez-Zamacona, Daniel Torregrosa, Guillermo Grindlay, Luis Gras, Carmen Pire, Juan Mora, Rosa María Martínez-Espinosa","doi":"10.1016/j.aca.2024.343453","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343453","url":null,"abstract":"<h3>Background</h3>Single particle inductively coupled plasma mass spectrometry (spICP-MS) is extensively employed for the characterization of biogenic selenium nanoparticles (SeNPs) produced by mesophilic microorganisms. Nevertheless, because halophilic microorganisms are also well-known to produce SeNPs, further research efforts are required to investigate spICP-MS applicability for characterizing such nanomaterials in hypersaline media. The goal of this work is to develop a methodology for characterizing SeNPs in hypersaline media by spICP-MS. To this end, plasma operating conditions, non-spectral interferences and calibration strategies were investigated. The proposed method was employed to investigate the capabilities of the halophilic archaea <em>Haloferax mediterranei</em> to produce SeNPs.<h3>Results</h3>By the appropriate selection of experimental conditions, SeNPs can be accurately analyzed in hypersaline media by spICP-MS. Unlike previous works in the literature, no differences in ionic signal were observed between SeNPs and dissolved Se and, hence, there is no need to apply any empirical corrector factor for obtaining accurate particle size distributions. Non-spectral interferences are mitigated by diluting the sample at least 1:10³ which allows the use of water standards. Size (30 nm) and particle (7 x 10⁵ particles mL^-1) detection limits were low enough to characterize biogenic SeNPs produced by halophilic microorganisms. The use of the optimized methodology reveals that <em>Haloferax mediterranei</em> can produce SeNPs when it is exposed to selenite up to 1 mM, but no formation is produced for selenate exposure. Depending on incubation parameters (selenite concentration and time), the particle median diameter ranged from 80-100 nm, whereas particle concentration varied from 0.8 to 1.9 x10¹³ particles mL^-1.<h3>Significance</h3>This represents the first methodology for characterizing biogenic SeNPs in hypersaline media by spICP-MS with accuracy and precision using non-matrix matched standards. It opens the opportunity to investigate the capabilities of halophilic microorganisms (e.g., <em>H. mediterranei</em>) to produce Se-based nanomaterials.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"117 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of β-Nuclides in Various Solutions Using Plastic Scintillation Microspheres","authors":"Hui Zhang, Ma Yan, Lina Ma","doi":"10.1016/j.aca.2024.343454","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343454","url":null,"abstract":"<h3>Background</h3>Scintillation cocktails cannot directly measure samples with extreme acidity or alkalinity in β-nuclide liquid scintillation analysis. Plastic scintillation microspheres (PSm), as a novel scintillation material, offer the potential to overcome these limitations by allowing direct mixing with a variety of solutions for measurement, particularly in challenging chemical environments.<h3>Results</h3>This study evaluated the performance of PSm in various chemical environments, including four acids (nitric, hydrochloric, sulfuric, and phosphoric acids), an alkaline solution (NaOH), a high-salinity solution, and methanol. PSm formed stable mixtures with most solutions and exhibited excellent chemical stability in high-concentration NaOH, with minimal chemiluminescence interference. However, concentrated nitric and sulfuric acids caused chemical reactions with PSm, leading to discoloration and potential color quenching. Efficiency calibration was performed using ultrapure water, allowing for accurate measurement of ¹⁴C activity across different solutions with simple efficiency corrections.<h3>Significance</h3>The findings of this study highlight the advantages of PSm for β-nuclide measurement in a variety of complex chemical environments. PSm's stability in highly acidic, alkaline, and saline conditions makes it a promising tool for applications such as radioactive waste monitoring and pharmacokinetic research, providing a reliable and efficient alternative to conventional scintillation cocktails.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"26 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel All-in-one Target-Powered Entropy-Driven Dynamic DNA Networks to Regulate the Activity of CRISPR/AsCas12a for Enhanced DNA Detection","authors":"Xingrong Li, Cuixiang Wang, Jiatong Chai, Hongmao Liu, Xinli Jiang, Yumei Li, Zhiqiang Li, Yirong Li","doi":"10.1016/j.aca.2024.343455","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343455","url":null,"abstract":"<h3>Background</h3>The non-enzyme autonomous DNA nanodevices have been developed to detect various analytes through the programmability of Watson-Crick base pairing. Nevertheless, by comparison with enzymatic biosensors, the usage of enzyme-free DNA networks to create biosensors for testing low amounts of targets is still subject to the finite number of cycles. Besides, these biosensors still require the incorporation of other amplification strategies to improve the sensitivity, which complicates the detection workflow and lacks of a uniform compatible system to respond to the target in one pot.<h3>Results</h3>Here, we put forward a novel way for rapid and sensitive DNA diagnostic via EDN (entropy-driven dynamic network) coupling with CRISPR/AsCas12a-powered amplification. In the absence of the target, the autonomous hybridization among the substrate and crRNA is kinetically hindered by enclosing complementary regions, which leads to the loss of the activation function of Cas12a. On the contrary, the target initiates the EDN, reconfiguring the activator strand from a duplex to branch construction, which provides a valid means to adjust the hybridization with crRNA, thereby controlling the indiscriminate collateral cleavage activities of the CRISPR/AsCas12a. Compared with the traditional EDN, synergistic activation between the EDN and the CRISPR catalyst could dramatically enhance the detection signal of the target in one pot. Furthermore, the proposed approach provides universal platforms through the rational functional and structural design of DNA assembly modules.<h3>Significance and Novelty</h3>Overall, this <u>t</u>arget-triggered <u>E</u>DN switches the activator strand to <u>r</u>egulate the activity of <u>A</u>sCas12a (called TERA), which showed more than two orders of magnitude sensitivity than the conventional Cas12a alone assay, resulting in a devisable universal CRISPR sensing platform that favours the fast, robust and one-pot detection of nucleic molecules.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"2 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a fully automated slurry sampling introduction system for GF-AAS and its application for the determination of cadmium in different matrices","authors":"Charlie Tobias, Lennart Gehrenkemper, Thomas Bernstein, Sven Schlau, Fabian Simon, Mathias Röllig, Björn Meermann, Marcus von der Au","doi":"10.1016/j.aca.2024.343460","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343460","url":null,"abstract":"<h3>Background</h3>Graphite Furnace-Atomic Absorption Spectrometry (GF-AAS) is a powerful technique for trace element analysis, offering high sensitivity and precision. However, its effectiveness is limited by sample preparation challenges for solid samples like soils and microplastics. Traditional methods include sample preparation, such as digestion, which is time-consuming and involves reagents, like acids, contributing to measurement uncertainty and higher carbon footprints. Slurry sampling allows direct analysis of suspensions, offering a more efficient alternative. However, maintaining suspension stability is challenging, requiring robust autosampler systems to streamline the process and enhance analytical performance.<h3>Results</h3>We present a novel autosampler extension for slurry sample introduction into GF-AAS. This system ensures suspension stability with a stirring device and closed vessels to prevent evaporation and contamination, incorporating a cooling unit to reduce solvent and analyte losses. It installs and removes in minutes without additional connections. Validation with cadmium analysis in BAM-U110 (Soil) and BAM-H010 (ABS) showed high reliability. For BAM-U110 (Soil), we achieved recovery rates of 94 % ± 13 % in water suspension. The recovery rate for BAM-H010 (ABS) was 104 % ± 11 % in acetonitrile suspension. These results demonstrate the system’s robustness, versatility, and accuracy for different matrices.<h3>Significance</h3>The autosampler extension helps solve key problems in trace element analysis of solid samples, making the process faster and more accurate. It works well with complex materials, making it useful for areas like microplastic or nanoparticle analysis. This improvement also helps meet regulations for monitoring environmental and polymer samples, offering a reliable and flexible tool for high-throughput analysis with fewer errors.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"14 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ nucleic acid amplification induced by DNA self-assembly for rapid and ultrasensitive detection of miRNA","authors":"Hongfei He, Xuewen Zhang, Meng Deng, Yan Zhou, Hongwei Pang, Hui Yang, Jiazhen Lyu, Yuxin Feng, Xiangqin Geng, Xiaolan Guo, Guangcheng Luo, Bin Guo","doi":"10.1016/j.aca.2024.343457","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343457","url":null,"abstract":"<h3>Background</h3>To improve the sensitivity and specificity of nucleic acid detection, coupling two or more signal amplification systems is a feasible pattern, such as nucleic acid isothermal amplification coupling genome-editing technology, and cascaded DNA self-assembly circuits. And representative signal amplification strategies include loop-mediated isothermal amplification (LAMP), clustered regularly interspaced short palindromic repeats/associated proteins (CRISPR/Cas) systems, and catalyzed hairpin assembly (CHA). However, these detection strategies often require the enrichment of intermediate products, the replacement of reaction conditions and the design of multiple probes, which may seriously affect the reliability of detection results.<h3>Results</h3>Herein, we propose a novel nucleic acid detection system which is named as catalyzed hairpin assembly (CHA) coupled with embedded primer triggered isothermal amplification (CEA for short). DNA self-assembly probes in CEA contain a specially designed primer. When target nucleic acid (e.g., miRNA) initiates CHA reaction (the first signal amplification), the self-assembly product of CHA will expose a primer (named as embedded primer). The embedded primer will trigger a special nucleic acid isothermal amplification in situ, then generate plenty of double-stranded DNA products in 30 min with varying lengths (the second signal amplification). Compared to that of a typical CHA reaction, the sensitivity of CEA has increased by three orders of magnitude and the detection limit is as low as 0.228 fM. Besides, it has excellent detection performance in cancer and stem cell samples.<h3>Significance</h3>By coupling embedded primer with DNA self-assembly system, a new nucleic acid detection system (CEA) with one-step operation and dual signal amplification has been successfully established. Compared with traditional dual signal amplification systems, CEA can not only significantly improve the reaction efficiency, but also greatly reduce the difficulty of detection system design and experimental operation.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"18 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrophilic-lipophilic balance copolymer composite nanofiber as an adsorbent for online solid phase extraction of three estrogens from water samples with column-switching prior to high-performance liquid chromatography","authors":"X.U. Tong, X.U. Ke, FANG Zhong Ze, C.H.E.N. LiQin","doi":"10.1016/j.aca.2024.343456","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343456","url":null,"abstract":"<h3>Background</h3>In recent years, the increasing accumulation of estrogen pollutants in the environment has raised concerns about their impact on human health, necessitating the development of highly sensitive detection methods for trace pollutant enrichment and analysis in environmental samples. Online pretreatment detection technology offers significant advantages over conventional offline techniques, including high automation, minimal human intervention, and improved efficiency. However, the key to successful implementation lies in the advancement of novel adsorbent materials<h3>Results</h3>Herein, we present a novel adsorbent material called polyacrylonitrile-hydrophilic-lipophilic balanced copolymer (PAN-HLB) composite nanofiber prepared via electrospinning for efficient online packed fiber solid phase extraction (PFSPE) of three estrogens from water samples. A rapid and highly sensitive online PFSPE-HPLC-FLD method was developed for the quantification of 17 β-Estradiol (E2), Estriol (E3), and 17-ethinylestradiol (EE2). The curves of the target analytes were prepared with good correlation coefficient values (<em>r</em>² &gt; 0.9900) in the range of 0.1-2 ng/mL. The limit of detection (S/N=3) was 0.05 ng/mL, the limit of quantitation (S/N=10) is 0.1 ng/mL. The recoveries of three estrogens were 94.0%∼110.3%, and the precisions (RSD) were less than 5%. The online PFSPE column, packed with the PAN-HLB composite nanofiber, exhibits exceptional stability and can be effectively reused for a minimum of 100 cycles.<h3>Significance</h3>This study presents the successful preparation of PAN-HLB composite nanofiber for the first time, demonstrating their efficacy as an innovative online pretreatment adsorbent. The proposed online pretreatment and detection approach offers remarkable benefits in terms of sensitivity, labor-saving, and cost-effectiveness that make it highly suitable for practical water sample analysis.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"322 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2D Zn-based metal-organic framework as an efficient electrochemiluminescence emitter: a novel inner filter effect-based ECL biosensor for trace detection of bisphenol A","authors":"Qingyuan Song, Xueling Shan, Ding Jiang, Wenchang Wang, Guohong Dai","doi":"10.1016/j.aca.2024.343416","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343416","url":null,"abstract":"The potential hazards of bisphenol A (BPA) to the environment have become a global concern. Herein, 2D Zn-based metal-organic framework nanosheet (2D Zn-MOF) and MnCO₃ nanocomposite (Zn-MOF-MnCO₃), an efficient electrochemiluminescence (ECL) probe was first synthesized and constructed for trace detection of BPA. Owing to the elimination of the aggregation-induced quenching (ACQ) effect of polycyclic aromatic hydrocarbons (PAHs), the leaf-like Zn-MOF exhibited a satisfactory ECL signal. The MnCO₃, which has excellent biocompatibility, showed excellent ECL efficiency in the presence of K₂S₂O₈. With the covalent binding of Zn-MOF and MnCO₃, we demonstrated that the ECL intensity and stability of Zn-MOF-MnCO₃ improved significantly. In addition, the inner filter effect (IFE) of Fe₃O₄-NH₂ NPs toward Zn-MOF-MnCO₃ had been confirmed to be the ECL quenching mechanism. Based on above strategies, the proposed ECL-IFE biosensor exhibited a trace detection ability of BPA in a wide linear range (10 fM ∼ 10 μM) with a low detection limit (4.2 fM). Further in-depth study confirmed the excellent repeatability, selectivity, and stability of sensors, which provided a fresh sensing platform for trace detection of BPA in the environment.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"57 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luminol/PtCo@rGO and Au@CNTs-based electrochemiluminescence cytosensor for ultrasensitive detection of breast cancer CTCs","authors":"Zhen Guo, Shenghang Jin, Meiying Yang, Luxuan Fu, Yan Ran, Yan Yu, Weizhong Wang","doi":"10.1016/j.aca.2024.343452","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343452","url":null,"abstract":"<h3>Background</h3>Breast cancer CTCs have recently been recognized as an emerging biomarker for liquid biopsy of breast cancer. In this work, based on two-dimensional (2D) noble metal PtCo@rGO nanozymes and Au@CNTs bioconjugates, a novel electrochemiluminescence (ECL) cytosensor was was developed in order to detect breast cancer CTCs (MCF-7) ultrasensitively.<h3>Results</h3>The PtCo@rGO nanozymes possessed large specific surface area and high efficiency peroxidase-like activity, which can be used as nanocarriers to anchor and catalyze luminol ECL emission efficiently. Moreover, the PtCo@rGO nanozymes have fractal nanostructures similar to that of CTCs and can capable of enhancing the adhesion of MCF-7 when assembled together with aptamers containing HS-modified epithelial specific cell adhesion molecules (EpCAM, S1). Importantly, the S1/Au@CNTs bioconjugates loaded on the glassy carbon electrode (GCE) can effectively capture MCF-7 cells. Benefiting from the above-mentioned advantages, the ECL cytosensor constructed for MCF-7 cells detection performed well with a wide linear range (2 to 1×10⁴ cells mL^-1) and a low limit of detection (1 cells mL^-1).<h3>Significance</h3>The designed ECL cytosensor could provide a promising platform for CTC-based liquid biopsy and have broad application prospects in breast cancer early diagnosis and prognostic monitoring.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"16 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}