Analytica Chimica Acta最新文献

{"title":"Capillary flow of hybrid mode for enhancement of flow rate on μTADs","authors":"Yixi Shi, Haonan Li, Hao Chen, Xionghui Li, Muyang Zhang, Qinghao He, Jie Zhou, Jiahua Zhong, Xinyi Chen, Huiru Zhang, Lok Ting Chu, Weijin Guo","doi":"10.1016/j.aca.2025.343996","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343996","url":null,"abstract":"<h3>Background</h3>Microfluidic paper-based analytical devices (μPADs) and microfluidic paper-based analytical devices (μTADs) have already found many successful applications in biosensing. These devices mainly utilize the autonomous capillary pumping of liquid for sample actuation. Capillary flow control is very interesting and necessary for μPADs and μTADs with point-of-care diagnostic applications. Many attempts have been made on μPADs while few attempts exist for μTADs. Methods for facile flow rate control on μTADs are needed for improving the performance of μTADs on point-of-care diagnostics.<h3>Results</h3>Here, we develop a method for increasing the capillary flow rate on μTADs. By incorporating hollow channels within μTADs that are encapsulated by thermal contraction tubes, we establish a hybrid capillary flow mode, which significantly enhances the flow rates in these devices. We investigate the influence of hollow channel size on the capillary flow rate, and find that the average flow rate increases with the hollow channel size. The average flow rate of the group with hollow channel size as 1200 μm is 12353% more than that of the control group. Then we choose the group with hollow channel size as 1200 μm for investigation of flow rate enhancement on high viscosity liquid samples (glycerol) and non-Newtonian liquid samples (whole blood). Moreover, we also show that programmed enhancement of capillary flow rate is possible by creating μTADs with a partial hollow channel along the threads.<h3>Significance</h3>The simplicity of our method enables it to be used for facile flow rate control on μTADs, and it works well for various and complex liquid samples. We believe that by combination with immunoassays, it has the potential in improving the performance of μTADs for point-of-care diagnostics in the near future.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"20 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-channel, real-time, long-term stable electrochemical immunosensor based on Au, Cu-vertical graphene for detection of carcinoembryonic antigen in tumor cells","authors":"Tingting Yao, Wei Li, Hongji Li, Xiuwei Xuan, Cuiping Li, Mingji Li","doi":"10.1016/j.aca.2025.344017","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344017","url":null,"abstract":"<h3>Background</h3>The accurate and rapid determination of a broad-spectrum tumor marker, carcinoembryonic antigen (CEA), in tumor cells, human tissues, and body fluids is important for the early diagnosis, drug development, efficacy evaluation, and prognosis tracking of cancer.<h3>Results</h3>In this study, a dual-channel electrochemical immunosensor was designed for the sensitive determination of CEA levels using an Au-AuCu-vertical graphene (VG) sensing platform. An AuCu bimetallic doping strategy was adopted to improve the biocompatibility of graphene with the cells, and Au nanoparticles were electrodeposited to firmly bind numerous CEA antibodies. The immunosensor exhibited a broad limit of linearity from 0.001 to 30000 pg mL⁻¹ and a low limit of detection of 0.28 fg mL⁻¹. This immunosensor exhibited excellent selectivity, reproducibility, and long-term stability. The developed Au-AuCu-VG-based immunosensor pen combined with self-designed electrochemical immunoassay software achieved high-precision real-time on-site analysis of CEA concentrations.<h3>Significance</h3>The proposed AuCu-VG electrode exhibited antibody-binding ability, inherent probe peak, and excellent binding of the Au NPs. A new dual-channel electrochemical immunoassay strategy was developed based on the AuCu-VG electrode, which could sensitively and reliably detect the real-time concentration of CEA released by tumor cells, such as MCF-7.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"58 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The power of trapped ion mobility for isotope tracer experiments","authors":"Karin Preindl, Chuqiao Chen, Supriya Murthy, Florian Gruber, Christian Freystätter, Thomas Weichhart, Thomas Stimpfl, Birgit Reiter, Arvand Haschemi, Gunda Koellensperger","doi":"10.1016/j.aca.2025.344005","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344005","url":null,"abstract":"<h3>Background</h3>Isotope tracing experiments in cellular metabolomics are challenged by the multiple isomers and in-source fragments, which need to be considered to obtain unbiased isotopologue ratio measurements. Thus, both, selectivity and sensitivity are key requirements for customized workflows. Trapped ion mobility spectrometry (TIMS) introduces an additional separation dimension to mass spectrometry, separating otherwise co-eluting isomers by measuring the ion mobility of a molecule<strong>.</strong> This study shows for the first time, the potential of this MS platform for accurate isotopologue assessment as showcased in isotope tracer experiments using mammalian cells.<h3>Results</h3>The validation exercise focused on spectral accuracy, precision, and metabolite detection capabilities and comprised independent measurements on an orbitrap-based platform. Hydrophilic interaction chromatography, in combination with TIMS-TOF-MS delivered excellent results, with a minimum trueness bias and excellent precision (CV%) between 0.3% and 6.4%. The ion mobility separation allowed for differentiation of the otherwise co-eluting isomers fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P). Overall, isotopologue distributions were in good agreement upon crossvalidation with the orbitrap platform.Finally, a proof-of-concept tracer study addressed the activity of the glycolysis and the pentose phosphate pathway (PPP) in resting and endotoxin activated macrophages. We confirmed an activation of glycolysis and PPP in LPS activated macrophages, but found a potentially reduced relative contribution of glucose-6-phosphate (G6P) to increased F6P pools. Our findings imply that TIMS is a powerful technology for the reliable measurements of isotope distribution analysis in metabolic tracing experiments. <strong>Significance</strong>: By implementation of ion mobility, it is now possible to generate distinct isotopologue patterns for G1P and F6P in isotope tracer experiments. F6P plays a crucial role in glycolysis and PPP, highlighting the importance of precise analytical measurements. This is particularly true for metabolic studies in immunology and cancer research.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"75 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Identification of Multiplexed Pathogens via a Two-Step Dual-Channel Fluorescence Turn-on Array","authors":"Xingliang Huang, Yang Yu, Huihai Li, Zhongzheng Xiong, Jinwu Wei, Dengqiong Yu, Hengfei Zhao, Liya Liao, Yong Li, Chao Guo, Chao Xu, Weiwei Ni, Jinsong Han, Xuejuan Gao, Hui Huang","doi":"10.1016/j.aca.2025.344012","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344012","url":null,"abstract":"Bacterial infections have been an increasingly serious threat to human health. However, the rapid identification of multiplexed bacteria remains challenging due to their intricate composition. Herein, we developed a two-step, dual-channel fluorescence “turn-on” sensor array that sequentially amplifies signals <em>via</em> Indicator Displacement Analysis (IDA) and Aggregation-Induced Emission (AIE). Three weakly fluorescent, positively charged conjugated fluorophores (<strong>A1</strong>–<strong>A3</strong>) with AIE properties were designed to form electrostatic complexes (<strong>C1</strong>–<strong>C3</strong>) with negatively charged graphene oxide (<strong>GO</strong>). Upon addition of bacteria, fluorophores were released from the electrostatic complexes <em>via</em> IDA, resulting in fluorescence turn-on. These fluorophores then aggregated on the bacterial surface, further enhancing fluorescence. This array accurately differentiated among 10 distinct bacterial strains, achieving 98.3% classification accuracy within 30 seconds. Finally, the approach facilitated semi-quantitative bacterial analysis, multiplex identification, and robust differentiation in artificial urine samples, presenting a promising method for early infectious disease diagnosis.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"21 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic characterisation of microplastics released from disposable medical devices using laser direct infrared spectroscopy","authors":"Chuanfeng Chen, Shanshan Du, Ziyan Liu, Wenfei Li, Fangbiao Tao, Xuejiao Qie","doi":"10.1016/j.aca.2025.343982","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343982","url":null,"abstract":"<h3>Background</h3>Human exposure to microplastics (MPs) is widespread, attracting significant attention from both the public and the scientific community. Although several direct and indirect exposure pathways have been investigated, the extent of MP exposure from disposable medical devices remains poorly understood and warrants further research.<h3>Results</h3>This work indicates that many MPs (10-30 μm) were released during the simulated use of disposable medical devices. Two common medical devices–disposable infusion tubes and blood needles–were selected as the research subjects. Analysis utilizing laser direct infrared (LDIR) revealed that plastic released from infusion tubes primarily consisted of polyamide (PA), polyvinyl chloride (PVC), and polyethene terephthalate (PET), with an average total number (ATN) of 11.8 particles/mL. MPs released from blood collection needles mainly consisted of polyurethane (PU) and PET, with an ATN of 82.7 particles/mL. For a 0.9% normal saline, the ATN released from the infusion tubes during the stimulating infusion scenario at room temperature (4 h) was approximately 16 particles/mL, primarily consisting of PA, PVC, and PET. Additionally, the release of MPs increased with rising temperatures. Under the same conditions, ATN release from the blood collection needles was approximately 84.4 particles/mL, mainly from PA, PVC, and PU.<h3>Significance</h3>This implies that MPs can enter the bloodstream directly through infusion tubes and blood collection needles, highlighting the need for greater attention to the risk of patient exposure.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"67 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen peroxide regulated split-type electrochemiluminescence sensing platform for non-invasive detection of gastric cancer-associated D-amino acids","authors":"Juan Li, Ming-Chun Lai, Ya-Meng Zhong, Ya-Ling Chen, Na Wu, Wei Chen, Hua-Ping Peng","doi":"10.1016/j.aca.2025.344010","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344010","url":null,"abstract":"Monitoring D-amino acids concentrations has essential implications for gastric cancer diagnosis and treatment, especially in the non-invasive detection of gastric cancer. However, it remains a challenge to establish a high-performance detection method for D-amino acids. Here, a highly sensitive and selective D-proline (D-Ala) ECL assay strategy via a 'turn-off-on' split-type electrochemiluminescence (ECL) platform has been proposed. We found that hydrogen peroxide (H₂O₂) could be used as an efficient etching agent to turn on the MnO₂/gold nanocluster (AuNC)-based ECL nanoswitch platform. Based on abovementioned characteristics, we extended it to the D-amino acids assay since the enzymatic reaction between D-Ala and D-amino acid oxidase (DAAO) generates H₂O₂. Based on the abovementioned characteristics, this ECL sensing platform achieved a preferable linear-dependent curve in the detection range of 1.0×10 ^{<strong>−</strong>10}∼1.0×10^{<strong>−</strong>3} mol L^{<strong>−</strong>1}, and realized the detection of D-Ala as low as 2.2×10^{<strong>−</strong>11} mol L^{<strong>−</strong>1} (S/N=3). Furthermore, the proposed ECL biosensor showed excellent selectivity, stability, and reproducibility. Together with its powerful performance, this strategy could test D-Ala in saliva samples, which suggested that this ECL assay platform shows great prospect in disease diagnosis. We ascribe the high sensitivity and good anti-interference capability of the sensor to the combination of specific enzyme catalysis reaction, high-efficiency split-type AuNC probe-based ECL technique and the highly etching efficiency of H₂O₂ to MnO₂ nanomaterials on electrode surface. In our perception, this H₂O₂ mediated split-type ECL sensing platform provides a viable tool in ECL based bioananlysis. Therefore, our proposed approach not only provides a strategy for developing a high-performance platform for D-Ala detection, but also establishes a framework for the detailed design and development of ECL platform for other biological assays.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"36 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rotary fluorescence array microfluidic chip for rapid detection of multiple pathogens in foods using dual-discs assisted recycling for signal amplification","authors":"Bailu Liu, Zhenli Xu, Zhijian Jia, Huihui Wu, Tianhua Li, Zhenzhong Yu, Ning Gan","doi":"10.1016/j.aca.2025.344014","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344014","url":null,"abstract":"<h3>Background</h3>The ability to rapidly and on-site detect multiple pathogens in food is crucial for food safety monitoring. The microfluidic chip integrated with aptamer (Apt) probes stands as a powerful tool for this venture. However, the utility of these chips has been constrained by the need for cumbersome fluid control devices, such as syringe pumps and bulky optical detectors, so the development of highly integrated integrated chips has become one of the priorities of biosensor development in recent years.<h3>Result</h3>In this work, a novel rotary fluorescence array microfluidic chip has been engineered for the simultaneous and rapid detection of three pathogens. The chip has three different detection regions and a double-layer structure composed of PDMS and glass. The Apts and its complementary DNA strands were immobilized on the gold nanoparticle layer in the detection region for pathogen identification, the fluorescence (FL) from the double-stranded staining solution added after the reaction was used for signal amplification. Different arrays of gold discs can be employed to identify various bacteria. This method enables the detection of <em>Staphylococcus aureus</em> (<em>S.A</em>), <em>Salmonella typhimurium</em> (<em>S.T</em>), and <em>Vibrio parahemolyticus</em> (<em>V.P</em>) in food samples within 30 min at a sensitivity of 7-39 CFU/mL.<h3>Significance</h3>The microfluidic chip has good specificity and sensitivity while avoiding cross interference, and the ratio form of quantification avoids background light interference. Different arrays can be used to identify different bacteria, which is of great significance for the rapid detection of a variety of foodborne pathogens in the field.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"38 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Well Colorimetric Sensor Array for Discrimination and Smartphone-assisted Detection of Catecholamines Based on Fe-Carbon Dots Nanozymes","authors":"Guang-Ming Bao, Dan-Dan Chen, Yi-Fan Xia, Zhi-Qiang Cai, Shun-Qiang Cui, Xia Wei, Zhen-Chong Dou, Yuan Yuan, Akimana Sandra, Hou-Qun Yuan","doi":"10.1016/j.aca.2025.343997","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343997","url":null,"abstract":"<h3>Background</h3>Catecholamines (CAs), such as noradrenaline (NE), adrenaline (AD), and dopamine (DA), are essential signaling mediators that regulate various physiological functions. Monitoring their levels is crucial for studying and diagnosing diseases, as abnormal concentrations are associated with numerous health conditions. However, distinguishing between these CAs is challenging due to their highly similar molecular structures.<h3>Results</h3>In this study, Fe-doped carbon dot-based nanozymes (<strong>Fe-CDs</strong>) with strong peroxidase-like activity were synthesized using a simple one-pot method. <strong>Fe-CDs</strong>-based sensing systems exhibit excellent stability, reproducibility, sensitivity (with detection limits of 26.6 nM for NE, 46.0 nM for AD, and 33.3 nM for DA), and anti-interference properties. A triple-channel single-well colorimetric sensor array was developed by collecting the absorbance at 20, 40, and 60 min as sensing units, enabling the effective differentiation and identification of various CAs.<h3>Significance</h3>The <strong>Fe-CDs</strong>-based system has proven capable of detecting CAs in real human urine and fetal bovine serum. Additionally, the <strong>Fe-CDs</strong>-based smartphone-assisted platform provides efficient, highly sensitive, and on-site CAs detection, making it highly promising for biomedical and diagnostic applications.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"231 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification of urinary stones using near-infrared spectroscopy and chemometrics: A promising method for intraoperative application","authors":"Ekaterina Boichenko ,&nbsp;Mikhail Paronnikov ,&nbsp;Anna Reznichenko ,&nbsp;Dmitry Korolev ,&nbsp;Vladimir Protoshchak ,&nbsp;Dmitry Kirsanov","doi":"10.1016/j.aca.2025.344007","DOIUrl":"10.1016/j.aca.2025.344007","url":null,"abstract":"<div><div>In low-invasive surgical treatment of urolithiasis, there is a need for an analytical method to determine the chemical composition of urinary stones in real-time mode, i.e., intraoperatively. While a thorough phase analysis can be done after the surgery, preliminary information about a target stone would be helpful for the specialists for choosing an optimal strategy of treatment and giving some immediate dietary or drug prescriptions to a patient. Near-infrared spectroscopy (NIRS) is a good candidate for such a method that can provide immediate results without obligatory sample preparation. Fiber optic probes, often used for acquiring near-infrared spectra, are compatible with surgical instrumentation. Chemometric algorithms can successfully resolve the complexity of NIR spectra, which consist of overlapped signals. For the first time, we applied NIRS in diffuse reflectance mode to classify three major types of urinary stones: oxalates, urates, and phosphates. To imitate the real conditions of a surgery, the NIR spectra were acquired not only under ambient conditions but also in saline medium. A trained and optimized multinomial classifier (Error Correcting Output Codes) showed an acceptable precision and recall for an independent validation dataset. Even considering the strong absorbance of saline, the calculated geometric mean was 94 %, 87 %, and 71 % for oxalates, urates, and phosphates, respectively. A first real-time approbation during a real surgery (percutaneous nephrolithotomy) demonstrated a compatibility of the suggested approach with the surgical protocols and a good agreement of the acquired NIR spectra and the results of reference X-ray phase analysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 344007"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmon-enhanced fluorescence sensor based on Au nanocages for sensitive detection of norepinephrine","authors":"Chenye Xu, Shilin Chen, Wen Sun, Rong Xu, Xiangyuan Yin, Dongsheng Guo, Yaqiong Yang","doi":"10.1016/j.aca.2025.343995","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343995","url":null,"abstract":"<h3>Background</h3>Norepinephrine (NE) as a crucial monoamine neurotransmitter in the central and sympathetic nervous system, plays an important role in different physiological and pathophysiological processes. Brain NE can modulate cerebrospinal fluid flux and neurovascular coupling, regulate cortical and hippocampal neuronal circuitry, and participate the immune system. In addition, the reduced concentration of NE in brain was currently deemed to be the internal reason of major depression. However, development of detection method of NE with high spatiotemporal resolution in living systems remains a great challenge.<h3>Results</h3>Herein, a plasmon-enhanced fluorescence (PEF) sensor based on Au nanocages (Aucages) were designed and prepared for ultra-sensitive detection of NE. Aucages with porous walls, hollow interior and systematically tunable localized surface plasmon resonance (LSPR) wavelengths (536 nm, 654 nm, 754 nm) were prepared to obtain a highly fluorescent enhancement of Au nanoclusters (Au NCs). Moreover, polyethylene glycol (PEG) with different molecular weight (1000, 5000, 10000 Da) were applied to control the distance between the Aucages and Au NCs. 3D-FDTD simulation results indicated that the fluorescence enhancement was primarily due to the internal and external enhanced electric field effects of Aucages. This sensor was applied for the turn-on detection of NE in commonly used clinical injectable norepinephrine bitartrate with the recovery rate of 98.06-105.34%. Meanwhile, real-time fluorescence imaging of NE at living pheochromocytoma (PC-12) cells was explored with a red-emitted fluorescence.<h3>Significance</h3>This study first employed Aucages with more “hot spot” for red-emitted Au NCs to realize fluorescence enhancement. It provides a new method for the development of more sensitive, accurate and convenient analysis of NE in clinical drug analysis, cell monitor and metabolism study.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"30 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}