Analytica Chimica Acta最新文献

{"title":"Multi-scale convolutional attention GRU network combined with improved CARS strategy to determine key elements in ores by XRF","authors":"Zenan Xiao ,&nbsp;Fusheng Li ,&nbsp;Wanqi Yang ,&nbsp;Shubin Lyu","doi":"10.1016/j.aca.2025.344494","DOIUrl":"10.1016/j.aca.2025.344494","url":null,"abstract":"<div><h3>Background</h3><div>X-ray fluorescence (XRF) technology is a promising method for estimating the metal element content in ores, which helps in understanding ore composition and optimizing mining and processing strategies. However, due to the presence of a large number of redundant features in XRF spectra, traditional quantitative analysis models struggle to effectively capture the nonlinear relationship between element concentration and spectral information of XRF, making it more difficult to accurately predict metal element concentrations. Thus, analyzing ore element concentrations by XRF remains a significant challenge.</div></div><div><h3>Results</h3><div>This study proposes a novel spectral variable selection method and a new model for quantitative analysis of metal elements in ore XRF. The variable selection method is composed of a heuristic optimization-based competitive adaptive re-weighted sampling method (CARS). The proposed quantitative analysis method integrates multi-scale convolutional attention with gated recurrent unit network (MSCA-GRU). Firstly, multi-scale convolutional attention uesd to reshape spectral feature importance and subsequently utilizes GRU network to extract potential nonlinear relationships in spectral variables. The experimental results show that the heuristic optimization-based CARS method outperforms the traditional CARS algorithm by effectively removing redundant features while retaining useful information. Furthermore, the MSCA-GRU model, compared to other advanced machine learning models, achieves high-precision quantitative analysis for Cu, Zn, and Pb, reaching the coefficient of determination (R²) of 0.9982, 0.9985, and 0.9970, respectively.</div></div><div><h3>Significance</h3><div>The proposed MSCA-GRU method combined with heuristic optimization-based CARS includes variable selection and quantitative analysis algorithms, effectively improving the accuracy of key elements quantification in ores. It provides a new method for accurately determining the concentration of metal elements, helping to simplify mineral exploration processes.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1373 ","pages":"Article 344494"},"PeriodicalIF":6.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of IgY antibody and its application in immunochromatographic assay for bongkrekic acid and its isomer","authors":"Bin Wang ,&nbsp;Xue-Ming Cao ,&nbsp;Ting-Ting Yun ,&nbsp;Xiao-Min Liang ,&nbsp;Yi-Na Lu ,&nbsp;Yu-Xin Zhong ,&nbsp;Zhen-Lin Xu","doi":"10.1016/j.aca.2025.344476","DOIUrl":"10.1016/j.aca.2025.344476","url":null,"abstract":"<div><div>Egg yolk immunoglobulin (IgY) has emerged as a promising alternative to monoclonal antibodies (mAbs) due to its facile extraction, higher yield, and greater tolerance to organic solvents. This work developed a selective IgY antibody against bongkrekic acid (BA) and isobongkrekic acid (IsoBA), the lethal toxins produced by <em>Burkholderia gladioli pv. Cocovenenans</em> (BGC), which led to severe food poisoning incidents and resulted in casualties. In detail, the purified cBA was first conjugated with a carrier protein to generate an immunogen and immunized hens, followed by an optimized purification protocol, attaining a high-affinity anti-BA IgY with minimal cross-reactivity. Based on the obtained anti-BA IgY, a rapid and sensitive immunochromatographic assay (ICA) was developed and integrated with a smartphone. The linear range of ICA was 0.72–4.41 μg/kg with a limit of detection (LOD) of 0.42 μg/kg. Recovery rates ranged from 82.2 % to 106.4 % in spiked rice flour, agaric, and tremella, and there was no statistically significant deviation from results obtained by LC-MS/MS in blind samples. Compared to mAb-based ICAs, IgY-based ICAs offer some significant advantages, including a non-invasive production process that adheres to ethical animal welfare standards, high production yields, and exceptional tolerance to organic solvents. These attributes position IgY as a viable alternative for future commercial ICA development.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1373 ","pages":"Article 344476"},"PeriodicalIF":6.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144766950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel NIR iridium(III) complex-based probe for accurate imaging of mitochondrial biothiols in ferroptosis-mediated lung ischemia-reperfusion injury","authors":"Lin Jiang ,&nbsp;Chaolong Liu ,&nbsp;Wenyu Wang ,&nbsp;Feng Li ,&nbsp;Qingming Ma ,&nbsp;Yong Sun","doi":"10.1016/j.aca.2025.344500","DOIUrl":"10.1016/j.aca.2025.344500","url":null,"abstract":"<div><h3>Background</h3><div>Lung ischemia-reperfusion injury (LIRI) is a pathological condition characterized by aggravated oxidative-inflammatory tissue damage that occurs upon blood flow restoration after ischemia. LIRI can lead to severe complications, including primary graft dysfunction in lung transplants and multi-organ failure. However, current treatments remain limited. Studies indicate that ferroptosis exacerbates LIRI through mitochondrial biothiols dyshomeostasis. Visualizing mitochondrial biothiols during ferroptosis is crucial for understanding LIRI pathology and developing new therapies. Nevertheless, reliable imaging methods to track these biothiols in ferroptosis-mediated LIRI are still lacking.</div></div><div><h3>Results</h3><div>In this study, we developed a biothiols-activated near-infrared (NIR) iridium(III) complex probe, termed NIR-Ir-BDS, for specifically and visually monitoring mitochondrial biothiols fluctuations in ferroptosis-mediated LIRI. NIR-Ir-BDS exhibits very weak NIR luminescence. Upon exposure to biothiols, the 2,4-dinitrobenzene sulfonamide (DBS) group of NIR-Ir-BDS undergoes rapid cleavage through a nucleophilic substitution reaction with the thiol group (-SH), yielding NIR-Ir-BH that exhibits intense NIR luminescence. The probe demonstrates a large Stokes shift, rapid response speed, excellent selectivity, high sensitivity, a satisfactory signal-to-noise ratio, good biocompatibility, and effective mitochondrial-targeting capability, enabling real-time monitoring of mitochondrial biothiols dynamics in living cells under pathological conditions. Furthermore, this study achieved, for the first time, the clear visualization of mitochondrial biothiols in mouse lung tissues during ferroptosis-mediated LIRI and systematically evaluated the in vivo therapeutic response to ferroptosis inhibition.</div></div><div><h3>Significance</h3><div>These findings reveal a pivotal association between ferroptosis and the pathogenesis of LIRI, demonstrating that targeted pharmacological intervention in this pathway substantially mitigates disease progression. The newly developed probe NIR-Ir-BDS can be used as a reliable imaging tool to elucidate mitochondrial biothiol pathology in ferroptosis-mediated LIRI, thereby facilitating the development of innovative therapeutic interventions for this disease.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1373 ","pages":"Article 344500"},"PeriodicalIF":6.0,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemically and Electromagnetically dual-enhanced COFs-Au@AgNPs SERS sensor integrated with deep learning for ultrasensitive detection of neonicotinoid pesticides","authors":"Xijun Wu,&nbsp;Xuan Zhao (赵,轩),&nbsp;Jiangtao Wang,&nbsp;Xuan Zhao (赵,煊),&nbsp;Shixin Wang,&nbsp;Yungang Zhang","doi":"10.1016/j.aca.2025.344501","DOIUrl":"10.1016/j.aca.2025.344501","url":null,"abstract":"<div><h3>Background</h3><div>With the development of modern agriculture, neonicotinoid pesticides have been widely used due to their high efficiency and strong systemic properties. However, excessive use leads to the accumulation of residues in the food chain, threatening the ecosystem and human health. Pesticide residues are easily accumulated in oilseed crops and become concentrated during the edible oil refining process. Long-term consumption of edible oils containing residues may pose health risks. Therefore, there is an urgent need to develop rapid and sensitive methods for the efficient detection of neonicotinoid pesticide residues in edible oils to ensure food safety.</div></div><div><h3>Results</h3><div>we developed a dual-mechanism-enhanced surface-enhanced Raman spectroscopy (SERS) sensor by integrating Covalent Organic Frameworks with Au@Ag nanoparticles (COFs-Au@AgNPs). Combined with Improved Informer (S-informer) model, this sensor enables trace detection of clothianidin (CTD), imidacloprid (IMD), and acetamiprid (AMP) in Extra Virgin Olive Oil (EVOO). The dual mechanism enhancement characteristics were revealed through Finite-Difference Time-Domain (FDTD) and Density Functional Theory (DFT): the COFs-Au@AgNPs composite system generates localized surface plasmon resonance (LSPR) to achieve electromagnetic enhancement |E/E₀|&gt;10²), while the mesoporous structure of COFs enhances the adsorption of pesticide molecules via intermolecular interactions (the adsorption energies (ΔE) range from −7.44 to −3.93 eV). The sensor's detection limits for CTD, IMD, and AMP are 6.31, 8.91, and 8.32 pM respectively, which are far below the maximum residue limits set by the U.S. EPA. The S-informer model demonstrated 100 % accuracy in classifying mixed pesticides, with superior quantitative prediction performance (R²p &gt; 0.999, RPD &gt;42.5).</div></div><div><h3>Significance</h3><div>This study developed a SERS sensor based on COFs-Au@AgNPs, which features a dual enhancement mechanism and integrates deep learning technology for detecting pesticide residues in lipid-rich matrices. The research provides a highly sensitive and reliable solution for monitoring pesticide contamination in food, while offering strong support for ecological protection and public safety.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1373 ","pages":"Article 344501"},"PeriodicalIF":6.0,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbonate esters as green alternatives in chromatographic separations","authors":"Alain Berthod, Sakil Islam, Daniel W. Armstrong","doi":"10.1016/j.aca.2025.344471","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344471","url":null,"abstract":"<h3>Background</h3>Carbonate esters are polar aprotic solvents that can be used to replace polar solvents: methanol, acetonitrile, or even apolar solvents in the mobile phases for liquid chromatography. Dimethyl, diethyl, and propylene carbonates (DMC, DEC, and PC) are not fully soluble in water.<h3>Results</h3>Twelve volume phase diagrams of water, the three carbonates, and methanol, ethanol, propanol, and acetonitrile were determined. They show that alcohols are much better carbonate/water mixing solvents than acetonitrile. DEC is the worst candidate as a green solvent. It needs too large amounts of mixing solvent to make a monophasic mobile phase usable in liquid chromatography. Also, it is the carbonate ester the most sensitive to water hydrolysis. The elution strength of carbonate esters is comparable to acetonitrile and stronger than methanol, but their viscosity is much higher. The more viscous carbonate ester containing mobile phases need higher working pressures, but the chromatograms did not necessarily show decreased peak efficiencies. Some selectivity changes are observed.<h3>Significance</h3>PC is highly preferable to DMC as a green organic modifier substitute. It seems to be the best green solvent substitute for methanol or acetonitrile if it is possible to work at a moderately higher temperature to compensate for the higher viscosity.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"15 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Pb2+ and Fe3+ ions in Wastewater using a Low Cost Portable Optical Sensor Derived from Green synthesized Metal Oxide Nanoparticle on a Paper Substrate","authors":"Akanksha Mishra, Anupam Kushwaha, Roli Verma","doi":"10.1016/j.aca.2025.344477","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344477","url":null,"abstract":"<h3>Background</h3>Water contamination is a global challenge, primarily due to heavy metal ions like lead (Pb²⁺), iron (Fe³⁺), cadmium (Cd²⁺), andmercury (Hg²⁺) as well as dyes. These pollutants enter the ecosystem from industrial waste and runoff, accumulate in the environment and pose a high risk to humans, animals and plants. Various sensors, such as colorimetric sensors, and electrochemical sensors have been developed to detect these ions and dyes. The portability and ease of use of colorimetric sensors makes them a preferred choice. The development of an eco-friendly paper-based colorimetric sensor is essential for the efficient detection and simultaneous removal of water contaminants.<h3>Results</h3>We present a novel, simple colorimetric paper-based sensor for the naked-eye detection of Pb²⁺ and Fe³⁺ ions using copper oxide nanoparticles (CuO NPs). This sensor exhibits high sensitivity, with detection limits of 2.2 × 10^-8M for Pb²⁺ and 6.6 × 10^-8M for Fe³⁺ that makes it on par with or exceeding that of previously reported methods. Exposing the single paper probe to different heavy metal ions, shows distinct color changes for Pb²⁺ and Fe³⁺ ions, while no significant color changes occur with other ions. Moreover, the absorbance behavior of the nanoparticles in the presence of different heavy metal ions further confirms their selectivity and enables quantitative analysis of the concentrations of these two ions. The synthesized CuO NPs degrade rhodamine 6G (Rh6G) dye by approximately 92% in water and maintain over 95% detection efficiency even after 20 days of storage, making it an efficient alternative to commercial sensors.<h3>Significance</h3>This study pioneers the detection of heavy metal ions and the removal of dye from contaminated water using green synthesized nanoparticlesbased probe. The analyses were conducted on both natural and laboratory samples. This low-cost, highly sensitive, selective, and eco-friendly paper sensor provides rapid results, making it ideally suited for on-site water quality monitoring.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"28 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144748043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Miniatured dual-field microscope for intelligent Marine algae detection, sizing and counting in a microfluidic chip","authors":"Dong Lv,&nbsp;Xiaoshi Han,&nbsp;Hui Chang,&nbsp;Zhiqian Li,&nbsp;Yang Wu,&nbsp;Junyan Zhang,&nbsp;Yongxin Song","doi":"10.1016/j.aca.2025.344496","DOIUrl":"10.1016/j.aca.2025.344496","url":null,"abstract":"<div><div>On-site and accurately detecting, sizing and counting living algae are greatly needed under International Ballast Water Convention, yet still challenging due to the lack of miniatured device. In this paper, a miniatured microscope that has both blue view field and fluorescence field was developed. Dual-view-field with one exciting light is achieved by using a beam splitter to direct the light into two mini cameras. Due to the strong intensity of the exciting blue light, the excited fluorescence light is overridden by the blue light and cannot be observed in the blue-field camera. To image the chlorophyll fluorescent light, a chlorophyll filter is placed before the fluorescence-field camera. An intelligent algorithm was developed for automatically sizing and counting the detected algae. It was experimentally found that both the total algae and the living algae can be clearly shown in the cameras. The counting accuracy is larger than 89 %. The maximum allowable detection speed is determined by the diameter of the algae, the frame rate (fps) and resolution of the two mini cameras. The total size of the device is 260 mm ∗ 260 mm ∗ 280 mm (length ∗ width ∗ height) and is thus suitable for on-site evaluation of the living status of algae in treated ship's ballast water.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1373 ","pages":"Article 344496"},"PeriodicalIF":6.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144748044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Adaptive and Label-Free Colorimetric Assay for EDTA Using Copper(II)-Aptamer Complexes as Soft Nanozymes","authors":"Shengjie Gao, Junjie Liu, Yu Mao, Lu Wang, Lei Zheng","doi":"10.1016/j.aca.2025.344498","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344498","url":null,"abstract":"<h3>Background</h3>Copper is a vital trace element that plays a crucial role in various physiological processes due to its ability to exist in multiple oxidation states. Inspired by natural enzymes, researchers have developed copper-based nanozymes that mimic enzyme functions, offering cost-effective and stable alternatives to traditional enzymes. Despite their promising properties, the design and synthesis of these nanozymes can be complex and challenging. To address this issue, this work explores an intuitive approach to construct “soft” nanozymes simply by binding Cu²⁺ with its aptamers to form Cu(II)-aptamer complexes.<h3>Results</h3>Using a screening strategy, we identified a specific aptamer against Cu²⁺, designated P8, that significantly enhanced peroxidase-like activity when complexed with Cu²⁺, demonstrated by a notably smaller <em>K</em>_<em>m</em> value. Furthermore, we investigated the effects of various lengths of a “molecular clamp” on the conformational dynamics of the P8 aptamer, which perturbs the encapsulation of Cu²⁺ and modulated its peroxidase activity. Our findings indicate that optimizing the molecular clamp to a moderate length (<em>N</em>_<em>d</em> = 20) can further enhance the peroxidase activity of the Cu²⁺-P8 complex. The peroxidase-like soft nanozymes based on copper(II)-aptamer complexes were finally utilized for label-free detection of EDTA. The developed colorimetric sensing method exhibits outstanding detection capabilities for EDTA in Sprite and mineral water samples, achieving an extended dynamic range that accommodates higher concentrations through the incorporation of molecular clamps.<h3>Significance and novelty</h3>This work highlights a novel and straightforward approach to generate and improve the enzymatic properties of copper-based nanozymes. The strategy to regulate the enzymatic activity effectively addresses the limitations of narrow detection ranges commonly observed in traditional methods.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"111 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eucalyptol-based dispersive liquid-liquid microextraction in conjunction with GC-MS for phthalate esters determination in bottled water samples","authors":"Aneta Bałdowska, Patrycja Makoś-Chełstowska, Justyna Płotka-Wasylka, Natalia Jatkowska","doi":"10.1016/j.aca.2025.344491","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344491","url":null,"abstract":"<h3>Background</h3>Endocrine-disrupting compounds (EDCs) rank among the most hazardous chemicals to human health. Accordingly, the accurate determination and continuous monitoring of EDCs in everyday products are critical to ensuring public health and safety. These efforts require advanced analytical procedures to detect specific EDCs in samples with diverse matrix compositions at extremely low concentration levels. Most of the analytical methods developed to date rely on the use of toxic organic solvents for sample preparation. However, current trends in analytical chemistry, in accordance with the principles of Green Analytical Chemistry, emphasise strategies aimed at reducing the environmental impact of such procedures.<h3>Results</h3>In this work, a new analytical procedure involving eucalyptol-based dispersive liquid-liquid microextraction (DLLME) coupled to gas chromatography-mass spectrometry (GC–MS) for selected phthalate esters (PAEs) determination in bottled water samples, including water dedicated to children, has been developed. The method was optimised using a single-objective approach and validated, yielding good validation parameters, including low limits of detection (0.090–0.24 μg·mL^-1) and quantification (0.27–0.72 μg·mL^-1). In addition, extraction efficiency ranged between 76 and 131% with relative standard deviations under 6% for most analysed compounds (except for DEHP, equal 11%). In all real water samples, the analytes were under the limit of detection (LOD).<h3>Significance</h3>This study introduces a novel, eco-friendly extraction procedure that eliminates the use of organic solvents, utilising eucalyptol as a green alternative. By addressing both environmental concerns and public health risks, this work represents a significant advancement in the field of green analytical chemistry.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"15 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superficially ordered porous silica microspheres with smaller mesopore for the separation of structural isomers in liquid chromatography","authors":"Yan Chen ,&nbsp;Yan-Xiang Wang ,&nbsp;Yan-Li Wang,&nbsp;Hai-Tao Lv,&nbsp;Ji-Ying Song,&nbsp;Qi Wu","doi":"10.1016/j.aca.2025.344495","DOIUrl":"10.1016/j.aca.2025.344495","url":null,"abstract":"<div><h3>Background</h3><div>The separation of structural isomers is always a challenging task for liquid chromatography because of their similar physicochemical property. Research has found that materials with regular microporous structures exhibit excellent isomer separation performance. However, as the most easily available chromatographic material, silica stationary phases with regular and small mesopore structure have not yet been prepared, and it remains to be confirmed whether narrow pores in silica materials have the enhancing effect on shape selectivity in the separation of structural isomers.</div></div><div><h3>Results</h3><div>SiO₂@SiO₂ core-shell stationary phase with ordered and small mesopore (3.5 nm) was prepared for the separation of structural isomers. The superficially ordered porous silica (SOPS) was synthesized by in-situ growth of radial pore structure on the surface of nonporous silica. To investigate the narrow pore effect on shape selectivity, the SOPS C18 stationary phases with different pore size (3.5 nm and 10.1 nm) were compared in terms of separation performance. Several groups of isomers with different linearity and planarity were chosen as probes to evaluate the shape selectivity of stationary phases. The stationary phase with smaller pore size presented better separation performance in certain circumstances. It was found SOPS C18–3.5 nm column had higher linearity selectivity which may come from its narrow pore channels with stronger limitation ability that imposes steric restrictions on the adsorption of molecules in a non-linear configuration and allows “slender” molecules to penetrate deep.</div></div><div><h3>Significance</h3><div>This work describes the design and preparation of SiO₂@SiO₂ stationary phase possessing regular and small mesopores, showing good separation performance for structural isomers. It reveals the narrow pore effect on enhancing shape selectivity, which may give inspirations for the design of stationary phases with ability of high-resolution separation of isomers.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1373 ","pages":"Article 344495"},"PeriodicalIF":6.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}