Analytica Chimica Acta最新文献

{"title":"Centrifugal microfluidic chip for multi-stage sorting and detection of microplastics at micron scale","authors":"Feifei Feng ,&nbsp;Wen-Qi Ye ,&nbsp;Xuecong Zhao ,&nbsp;Peng Wu ,&nbsp;Siwei Xiang ,&nbsp;Xing Fan ,&nbsp;Xiaohong Liu ,&nbsp;Hong Liu ,&nbsp;Wei Zhang","doi":"10.1016/j.aca.2025.343883","DOIUrl":"10.1016/j.aca.2025.343883","url":null,"abstract":"<div><h3>Background</h3><div>As an emerging contaminant, microplastics(MPs) have been widely detected in the environment, the environmental risks it poses are also becoming more prominent. Among them, micron-sized MPs have relatively higher biotoxicity, necessitating a technique for processing and analysis to separate them by particle size and analyze their composition. The most commonly used MPs separation technology at present is the membrane filtration, which is easily to cause membrane blockage and set error accumulation. Centrifugal microfluidic technology received great attention as a high efficiency, low error and simple operation technology, has great potential for the separation of MPs.</div></div><div><h3>Results</h3><div>In this paper, we have reported a multi-stage centrifugal microfluidic chip for the separation and detection of MPs (with diameter less than 63 μm). MPs of different diameters ranges were separated under the combination of Centrifugal force and Coriolis force, and orderly captured in four separation chambers according to their sizes. The capture rate of the microfluidic chip for polystyrene microspheres can reach about 87%. We also successfully separated MPs with irregular shapes. Under a rotation speed of 3500–4000 rpm, the maximum Pearson correlation coefficient between the volume equivalent diameters of the irregular MPs and the capture positions was about 0.84. Our proposed separation method was also applicable to MPs mixtures, which were successfully used for the separation of PVC, PC, and PS particles. The separated MPs can be directly identified to determine their chemical composition by Raman detection.</div></div><div><h3>Significance and novelty</h3><div>The experimental results demonstrate that our strategy is promising for separating and detecting MPs of different particle sizes in the environment and effectively overcomes the problem of error accumulation in traditional membrane separation methods. Furthermore, the device and methods developed in this study provide a foundational framework for formulating robust environmental risk assessment system.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343883"},"PeriodicalIF":5.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shear Flow Deformability Cytometry: A Microfluidic Method Advancing Towards Clinical Use-A review","authors":"Lija Fajdiga, Špela Zemljič, Tadej Kokalj, Jure Derganc","doi":"10.1016/j.aca.2025.343894","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343894","url":null,"abstract":"<h3>Background</h3>Shear flow deformability cytometry is an emerging microfluidic technique that has undergone significant advances in the last few years and offers considerable potential for clinical diagnostics and disease monitoring. By simultaneously measuring mechanical and morphological parameters of single cells, it offers a comprehensive extension of traditional cell analysis, delivering unique insight into cell deformability, which is gaining recognition as a novel biomarker for health and disease. Due to its operating principle, the method is particularly suitable for the clinical analysis of blood samples.<h3>Results</h3>This review focuses on the recent developments in shear flow deformability cytometry, which is a widely adopted variant of deformability cytometry. It has a strong potential for applications in clinical practice due to its robust and simple operation, demonstrated applications with whole blood samples, as well as its high throughput, which can reach approximately 1000 cells per second. We begin by discussing some basic factors that influence the mechanical properties of cells and give an overview of deformability cytometry and its operational principles for samples from blood, cultured cells and tissues. Next, we review recent clinically relevant applications in analysis of blood and cancer cells. Finally, we address key challenges to clinical adoption, such as regulatory approval, scalable manufacturing, and workflow integration, emphasizing the need for further validation studies to facilitate clinical implementation.<h3>Significance</h3>This article uniquely emphasizes the clinical relevance of microfluidic shear flow deformability cytometry, by giving an overview of mechanical and morphological biomarkers studied in clinically significant samples. In addition, it addresses critical barriers to clinical translation. By identifying these obstacles, this article aims to demonstrate the potential of deformability cytometry to bridge the gap between the research and the routine medical practice.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"41 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable isotope tracing reveals glucose metabolism characteristics of drug-resistant B-cell acute lymphoblastic leukemia","authors":"Rong Hu, Zhengwei Duan, Mengyao Wang, Mengting Liu, Yaoxin Zhang, Yanxi Lu, Yuhan Qian, Enjie Wei, Jianghua Feng, Pengfei Guo, Yang Chen","doi":"10.1016/j.aca.2025.343884","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343884","url":null,"abstract":"<h3>Background</h3>Adult B-cell acute lymphocytic leukemia (B-ALL) is a malignant hematologic tumor characterized by the uncontrolled proliferation of B-cell lymphoblasts in the bone marrow. Despite advances in treatment, including chemotherapy and consolidation therapy, many B-ALL patients experience unfavorable prognoses due to the development of drug resistance. The precise mechanisms governing chemotherapy resistance, particularly those related to metabolic reprogramming within tumors, remain inadequately elucidated.<h3>Results</h3>Nalm6/DOX cells exhibited significantly elevated levels of glucose, pyruvate, alanine, glutamine, and glycine compared to Nalm6 cells. Conversely, reduced levels of citrate, acetate, and leucine were observed in Nalm6/DOX cells. Upon exposure to the culture medium supplemented with tracer ¹³C₆-glucose, the Nalm6/DOX cells showed an increase in the abundance of ¹³C-alanine and a decrease in the levels of ¹³C-lactate, indicating impaired utilization of ¹³C-pyruvate. Combining β-chloro-alanine (ALTi) with DOX could decrease the drug resistance phenotype of Nalm6/DOX cells. The results demonstrated that glycolysis and tricarboxylic acid cycle were suppressed in Nalm6/DOX cells, while metabolic flux through the alanine and glutamine pathways was increased. Therefore, inhibition of alanine biosynthesis in Nalm6/DOX exhibits the potential to reverse drug resistance.<h3>Significance</h3>A new insight into the impact of metabolism on chemotherapy resistance in B-ALL has been gained through the use of stable isotope resolved metabolomics based on nuclear magnetic resonance and ultra-performance liquid chromatography/tandem mass spectrometry. This provides promising ways for the development of innovative therapeutic strategies to alleviate drug resistance and relapse in affected patients.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"17 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A portable and efficient strategy for ofloxacin detection using Ce-based MOF-loaded glucose oxidase and a personal glucose meter","authors":"Qianqian Wang ,&nbsp;Baoshan He ,&nbsp;Yao Liu ,&nbsp;Ligen Wu ,&nbsp;Wenhong Zhao ,&nbsp;Dongdong Xie ,&nbsp;Wenjie Ren ,&nbsp;Yiwei Xu","doi":"10.1016/j.aca.2025.343880","DOIUrl":"10.1016/j.aca.2025.343880","url":null,"abstract":"<div><div>Antibiotic residues in food and the environment pose significant risks to public health and safety, necessitating the development of rapid, portable, and efficient detection methods. Ofloxacin (OFL), a widely used antibiotic, is of particular concern due to its potential for contamination in milk and surface water. Current detection methods often require expensive instrumentation and complex procedures, limiting their applicability for on-site testing. This work addresses the critical need for a cost-effective, portable approach to reliably detect OFL residues. We developed a novel personal glucose meter (PGM)-based aptasensor utilizing a porous spherical cerium-based metal-organic framework (Ce-MOF) as a loading platform for glucose oxidase (GOx) and oligonucleotide sequences (Ce-MOF-GOx-cDNA). The hybrid probe, formed by conjugating Ce-MOF-GOx-cDNA with aptamer-modified magnetic beads, enabled specific recognition of OFL through nucleobase pairing. The sensor exhibited a detection range of 50 pg/mL to 500 ng/mL with a detection limit of 40 pg/mL under optimal conditions. The process showed excellent selectivity, stability, and reproducibility. Real-sample testing in spiked milk and surface water demonstrated recovery rates of 99.5% – 108%, with relative standard deviations of less than 4.7%. This study presents a portable and efficient strategy for detecting OFL residues using a PGM-based aptasensor. The method combines simplicity, rapid detection, and high sensitivity, offering significant potential for on-site applications in food and environmental safety monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343880"},"PeriodicalIF":5.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An azacrown ether-based near-infrared fluorescent probe for the detection of Pb2+ and its applications in food, environmental water, plant and animal samples","authors":"Yueyuan Zhang ,&nbsp;Huacong Wei ,&nbsp;Yu Li ,&nbsp;Zhuye Shang ,&nbsp;Run Zhang ,&nbsp;Zhiqiang Zhang ,&nbsp;Qingtao Meng","doi":"10.1016/j.aca.2025.343882","DOIUrl":"10.1016/j.aca.2025.343882","url":null,"abstract":"<div><h3>Background</h3><div>Lead ion (Pb²⁺), as a kind of heavy metal ion, is particularly harmful to human health and ecosystems due to its high toxicity and easy bioaccumulation. Fluorescent probes capable of selective and sensitive detection of Pb²⁺ are crucial for enabling rapid and on-site monitoring and regulation, thereby mitigating its adverse health and environmental impacts. Additionally, the development of fluorescence probes for the detection of Pb²⁺ in plant systems is rarely reported. Accordingly, the development of near-infrared (NIR) emission fluorescence probe for the detection of Pb²⁺ in food, environment and <em>in vivo</em> is of great significance.</div></div><div><h3>Results</h3><div>In this work, an azacrown ether-based NIR fluorescence probe <strong>LCE1</strong> was reported for the detection of Pb²⁺. Probe <strong>LCE1</strong> can generate 1:1 complex with Pb²⁺, resulting in the inhibition of ICT effect to reduce the fluorescence signal. <strong>LCE1</strong> exhibited many advantages, including NIR emission (<em>λ</em>_<em>em</em> = 670 nm), high selectivity and sensitivity (LOD = 0.34 μM) and fast response (30 s). The quantitative determination of Pb²⁺ in real food and water samples has been achieved with good recovery using <strong>LCE1</strong> as the probe. Concurrently, the on-site and rapid determination of Pb²⁺ in water sample was realized by smartphone-assisted <strong>LCE1</strong>-based test strip technology. Notably, the fluorescence imaging of Pb²⁺ in cells and animals has been successfully implemented using the probe <strong>LCE1</strong>. Most importantly, the fluorescence imaging of Pb²⁺ in Pb-hyperaccumulator plant samples has been successfully demonstrated.</div></div><div><h3>Significance</h3><div>LCE1 could provide new methods for understanding the physiopathological roles of Pb²⁺, evaluating food safety and selecting plants used to remediate soil contaminated by heavy metals.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343882"},"PeriodicalIF":5.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel fluorometric and colorimetric dual-mode sensor for AMI early diagnosis based on an ultrathin Fe-MOF-74 nanosheet with peroxidase mimic activity and fluorescence properties","authors":"Jinyan Liu ,&nbsp;Yong Wang ,&nbsp;Weijie Peng ,&nbsp;Bin Qiu ,&nbsp;Kwok-yin Wong ,&nbsp;Shuisheng Hu","doi":"10.1016/j.aca.2025.343800","DOIUrl":"10.1016/j.aca.2025.343800","url":null,"abstract":"<div><h3>Background</h3><div>Cardiac troponin I (cTnI) is a crucial diagnosis biomarker for acute myocardial infarction (AMI). Early accurate determination of the concentration of cTnI significantly decreases the death rate of AMI. Compared with classic methods, dual-mode sensors for cTnI determination could help reduce the false positive rate.</div></div><div><h3>Result</h3><div>In this work, an ultrathin Fe-MOF-74 nanosheet with fluorescence properties and peroxidase mimic activity was synthesized for the first time. Applying this nanosheet, a novel dual-mode sensor was developed to quantify cTnI in human serum. The ultrathin Fe-MOF-74 nanosheets catalyze the decomposition of hydrogen peroxide produced by glucose oxidase (GOx)-triggered enzyme-linked immunosorbent assay (ELISA) applying cTnI as an antigen target, to generate reactive oxygen species (ROS). 3,3′,5,5′-tetramethylbenzidine (TMB) can be oxidized by the generated free radicals, which simultaneously lead to the fluorescence quench of Fe-MOF-74 due to the inner filter effect (IFE). The correlation between the morphology of Fe-MOF-74 and its fluorescence intensity and peroxidase mimic activity was also investigated.</div></div><div><h3>Significance</h3><div>The sensors exhibited linearity with the concentration of cTnI in 10–2000 pg mL⁻¹ in both the fluorescence and visual mode with the detection limit of 6.4 pg mL⁻¹ and 8.4 pg mL⁻¹, respectively. It presenting good selectivity and anti-interference ability, can provide accurate and precise results in testing the concentration of cTnI in serum samples from patients in hospitals. It could be applied in the early diagnosis of AMI to reduce the incidence, disability, and mortality rates.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343800"},"PeriodicalIF":5.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a natural porcine-estrogen-receptor-based pseudo immunoassay for screening of 13 estrogens in milk and studying its recognition mechanism","authors":"Zu Qiang Jiang ,&nbsp;Jian Ping Wang ,&nbsp;Peng Lei Cui","doi":"10.1016/j.aca.2025.343881","DOIUrl":"10.1016/j.aca.2025.343881","url":null,"abstract":"<div><h3>Background</h3><div>There have been some immunoassays reported for screening of the residues of estrogens in milk, but these methods can only determine one drug because of the limited recognition abilities of the used antibodies. Due to the broad specific recognition ability, receptor can be used as “special antibody” to develop pseudo immunoassay. However, there has been no article reporting the use of estrogen receptor for determination of estrogens in foods of animal origin so far. Furthermore, the recognition mechanism of estrogen receptor for estrogens have not been thoroughly studied.</div></div><div><h3>Results</h3><div>In this study, a type of magnetic probe based on 17β-estradiol was synthesized that was used to produce the natural porcine estrogen receptor with ovarian as the source tissue. Then its recognition mechanisms for 13 estrogens were studied based on the optimal homological model. Results showed it only interacted with estrogens but did not interact with other steroid hormones. Then it was utilized as a broad specific recognition reagent to develop a direct competitive method on 96-well microplate for detection of the 13 drugs in milk. Due to the utilization of streptavidin-biotin labeled horseradish peroxidase as signal amplification system, the sensitivities for the 13 estrogens (limits of detection 0.007–0.0573 ng/mL) were improved for 33–100 folds in comparison with the use of conventional horseradish peroxidase system (limits of detection 0.31–3.53 ng/mL). This method showed no cross-reactivity with other steroid hormones, consistent with the docking result.</div></div><div><h3>Significance</h3><div>This is the first study reporting the use of magnetic probe to produce natural receptor from animal tissues, and this is also the first study reporting a natural estrogen-receptor-based method for multi-determination of estrogens in food sample. With the guidance of this study, more receptors and related analytical methods for other drugs or small molecules maybe are reported in the future.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343881"},"PeriodicalIF":5.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical and experimental solutions for Fourier transform infrared microspectroscopy measurements of microparticles: A case study on Quercus pollen","authors":"Florian Muthreich ,&nbsp;Eirik Almklov Magnussen ,&nbsp;Johanne Heitmann Solheim ,&nbsp;Valeria Tafintseva ,&nbsp;Achim Kohler ,&nbsp;Alistair William Robin Seddon ,&nbsp;Boris Zimmermann","doi":"10.1016/j.aca.2025.343879","DOIUrl":"10.1016/j.aca.2025.343879","url":null,"abstract":"<div><h3>Background</h3><div>FTIR microspectroscopy is a popular non-destructive technique for chemical analysis and identification of microparticles, such as microplastics, pollen, spores, microplankton organisms, sediments and microfossils. Unfortunately, measured spectra of microparticles are usually distorted by Mie-type scattering interferents thus hindering the analysis of spectral data. To retrieve chemical absorbance spectra, two different approaches are regularly employed: analytical (application of scatter-correction preprocessing methods), and experimental (measurement in an embedding matrix). The comparative studies of preprocessing spectral strategies are needed to determine pros and cons of these approaches, and when they are most suitable for use.</div></div><div><h3>Results</h3><div>We conducted the first-ever comparative study on 12 different analytical and experimental approaches for FTIR measurements of microparticles, as demonstrated on classification and chemical characterisation of pollen of four <em>Quercus</em> species. Individual pollen grains were measured on 1) microscope slides and 2) embedded in a paraffin-polyethylene (PEP) matrix. For analytical approaches, we have applied simple model-based algorithm (EMSC: extended multiplicative signal correction), Mie-theory model-based algorithm (ME-EMSC: Mie-extinction EMSC) and deep learning-based algorithm (DCNN: deep convolutional neural network). Moreover, we applied algorithms for the correction of the embedded spectra: fringe-correction EMSC and two different paraffin-correction EMSC algorithms. The best classification accuracy is obtained for simple preprocessing, where scattering information is not completely removed, as well as for complex algorithms where scattering information is parameterized and retained. In chemical characterisation studies, strong scattering signals hinder valuable chemical information, and it is imperative to suppress them either by embedding or by an analytical approach.</div></div><div><h3>Significance</h3><div>The results show that scattering spectral interferents are not necessarily detrimental for classification studies of biological microparticles. In fact, they have considerable diagnostic value even in closely related microorganisms due to species-specific physical properties. The results clearly show that analytical and experimental solutions for FTIR measurements of microparticles should be carefully selected, taking into account the origin of the microparticles (i.e., biological or artificial) and purpose of the study (classification or chemical characterisation).</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343879"},"PeriodicalIF":5.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A gold nanoflower particle-based immunochromatographic assay sensor for on-site detection of six species of Salmonella in water and food samples","authors":"Yinglin Wang ,&nbsp;Yafang Wu ,&nbsp;Youxue Wu,&nbsp;Zhaoyi Feng,&nbsp;Dezhi Li,&nbsp;Qing Liu","doi":"10.1016/j.aca.2025.343813","DOIUrl":"10.1016/j.aca.2025.343813","url":null,"abstract":"<div><h3>Background</h3><div><em>Salmonella</em> is a prevalent zoonotic pathogen that threatens food safety and human health. Owing to the large number of <em>Salmonella</em> species and their significant variations in pathogenicity and virulence, it is difficult to classify <em>Salmonella</em> strains quickly, which makes rapid detection of <em>Salmonella</em> outbreaks and research on foodborne diseases difficult.</div></div><div><h3>Results</h3><div>Therefore, in this study, an ICA sensor for the detection of multiple <em>Salmonella</em> strains with high pathogenicity based on broad-spectrum <em>Salmonella</em> antibodies was developed using AuNFs as probes. Compared with other <em>Salmonella</em> ICA sensors, the sensor was able to detect six different types of <em>Salmonella</em>. The ICA sensor had a visual LOD of 10⁴ CFU/mL for <em>S. Paratyphi A</em>, <em>S. Typhimurium</em>, <em>S. Paratyphi B</em>, <em>S. Saintpaul</em>, <em>S. Heidelberg</em> and <em>S. enterica</em>. The ICA sensor had no cross-reaction with 20 common foodborne pathogens, which could effectively avoid incorrect results caused by cross-reaction and delay accurate tracing of pathogenic bacteria. Moreover, the feasibility of the ICA sensor was verified by detecting <em>Salmonella</em> in spiked drinking water, orange juice, and milk. The ICA sensor achieved a visual detection limit of 10⁴ CFU/mL and detected as low as 1 CFU/mL in chicken and egg samples after 6–8 h of enrichment.</div></div><div><h3>Significance</h3><div>In conclusion, this sensor offers a rapid, cost-effective, and reliable solution for the on-site detection of multiple <em>Salmonella</em> strains, addressing critical needs in food safety and public health.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343813"},"PeriodicalIF":5.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of Ce3+ ions functionalized magnetic black phosphorus nanosheets for highly efficient enrichment of phosphopeptides","authors":"Dandan Jiang,&nbsp;Yangyang Li,&nbsp;Siyu Wu,&nbsp;Lan Lan,&nbsp;Jinghai Liu","doi":"10.1016/j.aca.2025.343878","DOIUrl":"10.1016/j.aca.2025.343878","url":null,"abstract":"<div><h3>Background</h3><div>The abnormal variation of phosphorylation can lead to many human diseases, such as Alzheimer's disease and cancer. Because of its high throughput and rapidity, mass spectrometry (MS)-based method has been widely used to characterize phosphopeptides/phosphoproteins in complex biological samples. However, the direct MS analysis for phosphopeptides is still a challenging task due to the complexity of biological samples and the signal suppression of abundant non-phosphopeptides. Therefore, an efficient enrichment platform for low-abundance phosphopeptide capture and detection is in great demand.</div></div><div><h3>Results</h3><div>In this study, Ce³⁺ ions functionalized magnetic black phosphorus nanosheets were successfully synthesized and characterized. The prepared magnetic material had high surface area (185.80 m² g⁻¹), good hydrophilicity (12.15°), and magnetic property (33.38 emu g⁻¹). The magnetic material provided abundant affinity sites for phosphopeptide enrichment through immobilized metal ion affinity chromatography (IMAC). By combining MS analysis, the method exhibited satisfactory performance, including high sensitivity (0.1 fmol), good selectivity (<em>α</em>-casein: <em>β</em>-casein: BSA = 1: 1: 5000), and high recovery (87.2 %). The method was applied to enrich and detect phosphopeptides in skimmed milk, human saliva, serum, and A549 cell lysate, proving its feasibility for phosphopeptide analysis. Additionally, the method provided the sequence motifs of the captured phosphopeptides and the biological functions of the phosphoproteins in A549 cell lysate.</div></div><div><h3>Significance</h3><div>This work presented a facile analysis platform for phosphopeptides, including sample preparation, enrichment process, and MS detection. The analysis strategy was successfully adopted for capturing and analyzing phosphopeptides from stranded proteins and complex bio-samples. This work presents a novel approach for the design and construction of black phosphorus-based adsorbents in phosphoproteomics research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343878"},"PeriodicalIF":5.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}