Analytica Chimica Acta最新文献

{"title":"Carbon quantum dots-based fluorescent probe for sensitive and selective detection of glutathione in bacteria and cells","authors":"Lina Wu ,&nbsp;Huangpei Xiao ,&nbsp;Jiaxin Xue ,&nbsp;Xiaoyun Lin ,&nbsp;Zhenmao Zhu ,&nbsp;Ailin Liu ,&nbsp;Liqing Lin","doi":"10.1016/j.aca.2025.343983","DOIUrl":"10.1016/j.aca.2025.343983","url":null,"abstract":"<div><h3>Background</h3><div>The sensitivity of 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) method may not be enough to detect low concentration glutathione (GSH), especially in complex samples, which limits its application in some biologic scenarios. Fluorescent carbon quantum dots (CQDs) have garnered significant interest in bioanalytical applications due to their unique properties, particularly the sensitivity in the detection of complex samples.</div></div><div><h3>Results</h3><div>In this work, carbon quantum dots (TOCDs) were prepared via pyrolysis method of <span>d</span>-threonine and <em>o</em>-phenylenediamine. Considering advantages of selectivity of DTNB and sensitivity of TOCDs, a simple, fast and sensitive fluorescent sensor based on TOCDs and DTNB was fabricated for selective detection of GSH. In the presence of GSH in the mixture of TOCDs and DTNB, –SH of GSH can interact with DTNB and subsequently affording 5-thio-2-nitrobenzoate (TNB), which can strongly absorb excitation light of TOCDs and lead to fluorescence quenching of TOCDs. As a consequence, quantitative screening of GSH can be achieved through the fluorescence intensity of TOCDs was decreased after the addition of GSH in the system of TOCDs and DTNB. Under optimized conditions, good linear relationships in the range of 2.5–40 nM, 40–150 nM and 150–1000 nM for GSH. TOCDs can effectively detect GSH with a limit of detection (LOD) 1.5408 nM. The feasibility of the TOCDs probe was verified through non-invasive detection of reduced glutathione (GSH) in bacteria and cells. The fluorescence of TOCDs in different living bacteria/cells were yellow fluorescence, while the fluorescence could be ignored after joining GSH and DTNB. The mechanism about quench phenomenon of the fluorescence intensity between TOCDs and DTNB/GSH was the inner-filter effect. These results demonstrated that the presented fluorescent sensor could be a promising sensing platform for GSH analysis.</div></div><div><h3>Significance</h3><div>This sensing strategy could be expanded to more access to the GSH-induced acute toxicity and disease. The yellow fluorescence carbon quantum dots demonstrated its potential application on GSH detection in bacteria and cells.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 343983"},"PeriodicalIF":5.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical fingerprint of bacteriophages by infrared nano-spectroscopy","authors":"Yue Cao ,&nbsp;Dipesh Khanal ,&nbsp;Adrian Cernescu ,&nbsp;Hak Kim Chan","doi":"10.1016/j.aca.2025.344026","DOIUrl":"10.1016/j.aca.2025.344026","url":null,"abstract":"<div><div>Bacteriophage (phages) are naturally occurring nanoscale antimicrobial agents that can self-replicate at infection sites and selectively eliminate pathogenic bacteria. Significant heterogeneity exists in phage properties such as morphology, protein and nucleic acid composition, subject to the strain, state, and environment of the phage source. However, current techniques fall short in accurately mapping the chemical compositions of individual phages. A thorough understanding of this heterogeneity is essential to elucidate the difference between phage types and their stability, which may impact phages as effective therapeutic agents. We propose using scattering scanning near-field optical microscopy (s-SNOM) as an innovative method to map the chemical composition of phages at the nanoscale. The strength of this method lies in its label-free, ultra-high sensitivity that measures individual phage chemical heterogeneity. Additionally, s-SNOM is ideal for thermally sensitive phages, as it detects light scattered by nanoscale specimens without relying on thermal expansion. New insights from this method into phage chemical composition will profoundly impact our understanding of phage biology and optimise phage formulation for therapeutic use.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 344026"},"PeriodicalIF":5.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive amperometric immunosensor for pathogen antigen based on MoS2@AuNPs assembling dual-peptide as bioprobes with significant dual signal amplification","authors":"Zongmei Zheng ,&nbsp;Mingyang Wang ,&nbsp;Jinlong Yuan ,&nbsp;Junchong Liu ,&nbsp;Haipeng Yu ,&nbsp;Zhonghuang Yang ,&nbsp;Wanjian Liu ,&nbsp;Aihua Liu","doi":"10.1016/j.aca.2025.344015","DOIUrl":"10.1016/j.aca.2025.344015","url":null,"abstract":"<div><div>It is crucial to timely and accurately identify the causative virus for early treatment and urgent prevention. Viral antigen detection can identify those people who are most likely at risk of spreading the disease, but most based on antibodies with limited stability and sensitivity. Peptides offer several advantages over antibodies, such as low cost, smaller size and good stability. The development of electrochemical immunoassay using specific peptide probes have the merits of good sensitivity and selectivity as well as good stability. Herein we report an amperometric immunosensor using peptides as capture probe and recognition probe. The molecular docking suggests that the two peptides of Pi (sequence: NFWISPKLAFALGGGKKKSC) and FK11 (sequence: WFLNDSELISML), bioscreened from phage display, bind to <em>N</em>-terminal domain of SARS-CoV-2 spike protein (SP). The peptide Pi is assembled on MoS₂@AuNPs modified electrode to capture SARS-CoV-2 SP, which is recognized by peptide FK11-displayed phage to form Pi/SARS-CoV-2 SP/FK11-phage sandwich. Then <em>anti</em>-M13 phage conjugated horseradish peroxidase (HRP) (anti M13-HRP) was introduced to recognize the phage capsid protein pVIII to form M13 phage/anti M13-HRP to enrich thousands of HRP, which can further electrochemically catalyze H₂O₂ reduction at highly conductive MoS₂@AuNPs at − 0.35 V. Then amperometric immunosensor was constructed with linear range of 0.1–5000 pg/mL SARS-CoV-2 SP and detection limit of 0.074 pg/mL. The sensor also has good selectivity, batch reproducibility and stability, capable of detecting down to 10 transducing units/mL SARS-CoV-2 pseudoviruses. This work represents the first example of dual-peptide probes based sandwich-type electrochemical immunosensor integrated with dual signal amplification, which may provide a cost-effective assay platform in detecting real SARS-CoV-2 viruses for early diagnosis. The flexible and modular strategy can be extended to develop other type biosensors for a wide range of applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 344015"},"PeriodicalIF":5.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point-of-care colorimetric biosensor for H2O2 and glucose detection utilizing the peroxidase-like activity of 2D bimetallic metal organic framework nanosheets","authors":"Yasmeen M. Abdelfattah ,&nbsp;Amr M. Mahmoud ,&nbsp;Noha I. Abdelaziz ,&nbsp;Dina A. El Mously","doi":"10.1016/j.aca.2025.343993","DOIUrl":"10.1016/j.aca.2025.343993","url":null,"abstract":"<div><h3>Background</h3><div>The applications of natural enzymes are vast, limited only by their protein nature. Therefore, the development of artificial enzyme mimetics, nanozymes, which are stable and have improved activity, has become indispensable for biomedical and diagnostic purposes. Nanozymes have developed into an emergent topic combining nanotechnology and biology due to their vast range of potential uses. In comparison to natural peroxidase, peroxidase-imitating nanozymes have distinct benefits in terms of high stability and low cost for applications in bioanalysis and environmental remediation. The use of metal-organic framework nanoparticles has exhibited enhanced catalytic and enzymatic performance.</div></div><div><h3>Results</h3><div>In the current work, we present a strategy for synthesizing 2D Ni/Co MOF nanoparticles that have been anchored onto carboxymethyl cellulose (CMC). The resulting composite (Ni/Co-MOF@CMC) 2D nanosheets exhibit a high surface area and abundant catalytic sites, greatly amplifying their peroxidase-like catalytic performance. Additionally, these 2D bimetallic MOFs mimic the peroxidase activity, demonstrated by the distinctive yellow colour upon the oxidation of <em>o</em>-Phenylenediamine (OPD) by hydrogen peroxide. This newly synthesized 2D bimetallic MOF provides a straightforward, simple, selective, and sensitive colorimetric analysis technique for the determination of hydrogen peroxide and glucose. H₂O₂ could be efficiently detected with a linear range of 10 μM–800 μM and a lower detection limit of 3.28 μM. With the potential to detect minute glucose concentrations as low as 200 μM within a linear range of 200 μM–600 μM.</div></div><div><h3>Significance and novelty</h3><div>This work demonstrates the significant novelty of applying an RGB colour sensor (TCS34725) for the quantitative measurement of H₂O₂ and glucose which holds great potential as a point-of-care platform for diabetic patients. Consequently, our approach broadens the use of MOFs in biosensing and presents a viable substitute for affordable, and easily accessible diabetes monitoring. These 2D bimetallic MOFs are promising materials for glucose detection applications, expanding the utility of MOFs to include biosensor applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1356 ","pages":"Article 343993"},"PeriodicalIF":5.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating NMR and multi-LC-MS-based untargeted metabolomics for comprehensive analysis of blood serum samples","authors":"Tereza Kacerova,&nbsp;Elisabete Pires,&nbsp;John Walsby-Tickle,&nbsp;Fay Probert,&nbsp;James S.O. McCullagh","doi":"10.1016/j.aca.2025.343979","DOIUrl":"10.1016/j.aca.2025.343979","url":null,"abstract":"<div><h3>Background</h3><div>Mass spectrometry (MS) and nuclear magnetic resonance (NMR) have emerged as pivotal tools in biofluid metabolomics, facilitating investigation of disease mechanisms and biomarker discovery. Despite complementary capabilities, these techniques are rarely combined, although their integration is often beneficial. Typically, different sample preparation approaches are used, and compatibility challenges potentially arise due to the requirement for deuterated buffered solvents in NMR but not MS techniques. Additionally, MS-based approaches necessitate protein removal from samples whilst in NMR proteins can be potentially useful biomarkers. In this study, we developed a blood serum preparation protocol enabling sequential NMR and multi-LC-MS untargeted metabolomics analysis using a single serum aliquot in a research discovery setting.</div></div><div><h3>Results</h3><div>We analysed human serum samples using various untargeted NMR and multi-LC-MS platforms to assess the impact of deuterated solvents and buffers on detected compound-features. Employing multiple LC-MS profiling approaches, we observed no evidence of deuterium incorporation into metabolites following sample preparation with deuterated solvents. Furthermore, we demonstrated that buffers used in NMR were well tolerated by LC-MS. Protein removal, involving both solvent precipitation and molecular weight cut-off (MWCO) filtration, was identified as a primary factor influencing metabolite abundance. Our findings led to the development and validation of a serum sample preparation protocol enabling a combined NMR and multi-LC-MS analysis.</div></div><div><h3>Significance</h3><div>Using a single clinical serum aliquot for simultaneous untargeted profiling via NMR and multi-LC-MS represents a highly efficient alternative to current methods. This approach reduces sample volume requirements and substantially expands the potential for broader metabolome coverage. Our study offers comprehensive insights into the impact of sample preparation on complex metabolic biofluid profiles, highlighting the compatibility and complementarity of LC-MS and NMR in metabolomics research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1356 ","pages":"Article 343979"},"PeriodicalIF":5.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosted Synergistic Catalytic Performance of Pt Single-Atom Catalyst NiS2/Pt on Wearable Hydrogel Biosensor for Sweat Lactic Acid Analysis","authors":"Cuncun Wang ,&nbsp;Yong Zhang ,&nbsp;Xin Zeng ,&nbsp;Changpeng Jin ,&nbsp;Yiyi Liu ,&nbsp;Mei Yang ,&nbsp;Danqun Huo ,&nbsp;Changjun Hou","doi":"10.1016/j.aca.2025.343971","DOIUrl":"10.1016/j.aca.2025.343971","url":null,"abstract":"<div><h3>Background</h3><div>Lactic acid is an important biochemical marker for monitoring tissue oxidation, as its buildup can lead to soreness, fatigue, and potentially serious. Sweat lactic acid detection typically relies on lactic acid oxidase, but the limitations of natural enzymes restrict their use.</div></div><div><h3>Results</h3><div>A Pt single-atom catalyst (NiS₂/Pt) supported on NiS₂ was prepared via hydrothermal and photochemical fixation methods, and then modified onto a PI flexible electrode. This was integrated with a hydrogel GO/EG-SPB, which exhibits excellent mechanical properties and a PDMS microfluidic channel modified with 2% Silwet-L77. A sensitive, flexible electrochemical sensing platform was constructed for detecting sweat lactic acid. Through density functional theory (DFT) calculations, the optimal adsorption configurations and lowest adsorption energies of LA on the NiS₂/Pt surface were obtained, revealing the catalytic mechanism. The synergistic catalytic effect between Pt single atoms and surrounding Ni atoms significantly improves the sensor’s performance. Based on these unique advantages, the flexible sensing platform shows excellent sensing performance for sweat LA detection, including high selectivity, low detection limits (7.02 μM) and a wide linear range (10 μM - 10 mM).</div></div><div><h3>Significance</h3><div>The NiS₂/Pt prepared in this study has been successfully applied to accurate and sensitive lactate detection in sweat, which has promising applications in health monitoring and wearable technologies.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 343971"},"PeriodicalIF":5.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capillary flow of hybrid mode for enhancement of flow rate on μTADs","authors":"Yixi Shi ,&nbsp;Haonan Li ,&nbsp;Hao Chen ,&nbsp;Xionghui Li ,&nbsp;Muyang Zhang ,&nbsp;Qinghao He ,&nbsp;Jie Zhou ,&nbsp;Jiahua Zhong ,&nbsp;Xinyi Chen ,&nbsp;Huiru Zhang ,&nbsp;Lok Ting Chu ,&nbsp;Weijin Guo","doi":"10.1016/j.aca.2025.343996","DOIUrl":"10.1016/j.aca.2025.343996","url":null,"abstract":"<div><h3>Background</h3><div>Microfluidic paper-based analytical devices (μPADs) and microfluidic paper-based analytical devices (μTADs) have already found many successful applications in biosensing. These devices mainly utilize the autonomous capillary pumping of liquid for sample actuation. Capillary flow control is very interesting and necessary for μPADs and μTADs with point-of-care diagnostic applications. Many attempts have been made on μPADs while few attempts exist for μTADs. Methods for facile flow rate control on μTADs are needed for improving the performance of μTADs on point-of-care diagnostics.</div></div><div><h3>Results</h3><div>Here, we develop a method for increasing the capillary flow rate on μTADs. By incorporating hollow channels within μTADs that are encapsulated by thermal contraction tubes, we establish a hybrid capillary flow mode, which significantly enhances the flow rates in these devices. We investigate the influence of hollow channel size on the capillary flow rate, and find that the average flow rate increases with the hollow channel size. The average flow rate of the group with hollow channel size as 1200 μm is 12353% more than that of the control group. Then we choose the group with hollow channel size as 1200 μm for investigation of flow rate enhancement on high viscosity liquid samples (glycerol) and non-Newtonian liquid samples (whole blood). Moreover, we also show that programmed enhancement of capillary flow rate is possible by creating μTADs with a partial hollow channel along the threads.</div></div><div><h3>Significance</h3><div>The simplicity of our method enables it to be used for facile flow rate control on μTADs, and it works well for various and complex liquid samples. We believe that by combination with immunoassays, it has the potential in improving the performance of μTADs for point-of-care diagnostics in the near future.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 343996"},"PeriodicalIF":5.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-channel, real-time, long-term stable electrochemical immunosensor based on Au, Cu-vertical graphene for detection of carcinoembryonic antigen in tumor cells","authors":"Tingting Yao ,&nbsp;Wei Li ,&nbsp;Hongji Li ,&nbsp;Xiuwei Xuan ,&nbsp;Cuiping Li ,&nbsp;Mingji Li","doi":"10.1016/j.aca.2025.344017","DOIUrl":"10.1016/j.aca.2025.344017","url":null,"abstract":"<div><h3>Background</h3><div>The accurate and rapid determination of a broad-spectrum tumor marker, carcinoembryonic antigen (CEA), in tumor cells, human tissues, and body fluids is important for the early diagnosis, drug development, efficacy evaluation, and prognosis tracking of cancer.</div></div><div><h3>Results</h3><div>In this study, a dual-channel electrochemical immunosensor was designed for the sensitive determination of CEA levels using an Au–AuCu-vertical graphene (VG) sensing platform. An AuCu bimetallic doping strategy was adopted to improve the biocompatibility of graphene with the cells, and Au nanoparticles were electrodeposited to firmly bind numerous CEA antibodies. The immunosensor exhibited a broad limit of linearity from 0.001 to 30000 pg mL⁻¹ and a low limit of detection of 0.28 fg mL⁻¹. This immunosensor exhibited excellent selectivity, reproducibility, and long-term stability. The developed Au–AuCu-VG-based immunosensor pen combined with self-designed electrochemical immunoassay software achieved high-precision real-time on-site analysis of CEA concentrations.</div></div><div><h3>Significance</h3><div>The proposed AuCu-VG electrode exhibited antibody-binding ability, inherent probe peak, and excellent binding of the Au NPs. A new dual-channel electrochemical immunoassay strategy was developed based on the AuCu-VG electrode, which could sensitively and reliably detect the real-time concentration of CEA released by tumor cells, such as MCF-7.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 344017"},"PeriodicalIF":5.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The power of trapped ion mobility for isotope tracer experiments","authors":"Karin Preindl ,&nbsp;Chuqiao Chen ,&nbsp;Supriya Murthy ,&nbsp;Florian Gruber ,&nbsp;Christian Freystätter ,&nbsp;Thomas Weichhart ,&nbsp;Thomas Stimpfl ,&nbsp;Birgit Reiter ,&nbsp;Arvand Haschemi ,&nbsp;Gunda Koellensperger","doi":"10.1016/j.aca.2025.344005","DOIUrl":"10.1016/j.aca.2025.344005","url":null,"abstract":"<div><h3>Background</h3><div>Isotope tracing experiments in cellular metabolomics are challenged by the multiple isomers and in-source fragments, which need to be considered to obtain unbiased isotopologue ratio measurements. Thus, both, selectivity and sensitivity are key requirements for customized workflows. Trapped ion mobility spectrometry (TIMS) introduces an additional separation dimension to mass spectrometry, separating otherwise co-eluting isomers by measuring the ion mobility of a molecule<strong>.</strong> This study shows for the first time, the potential of this MS platform for accurate isotopologue assessment as showcased in isotope tracer experiments using mammalian cells.</div></div><div><h3>Results</h3><div>The validation exercise focused on spectral accuracy, precision, and metabolite detection capabilities and comprised independent measurements on an orbitrap-based platform. Hydrophilic interaction chromatography, in combination with TIMS-TOF-MS delivered excellent results, with a minimum trueness bias and excellent precision (CV%) between 0.3 % and 6.4 %. The ion mobility separation allowed for differentiation of the otherwise co-eluting isomers fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P). Overall, isotopologue distributions were in good agreement upon crossvalidation with the orbitrap platform.</div><div>Finally, a proof-of-concept tracer study addressed the activity of the glycolysis and the pentose phosphate pathway (PPP) in resting and endotoxin activated macrophages. We confirmed an activation of glycolysis and PPP in LPS activated macrophages, but found a potentially reduced relative contribution of glucose-6-phosphate (G6P) to increased F6P pools. Our findings imply that TIMS is a powerful technology for the reliable measurements of isotope distribution analysis in metabolic tracing experiments. <strong>Significance</strong>: By implementation of ion mobility, it is now possible to generate distinct isotopologue patterns for G1P and F6P in isotope tracer experiments. F6P plays a crucial role in glycolysis and PPP, highlighting the importance of precise analytical measurements. This is particularly true for metabolic studies in immunology and cancer research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 344005"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid identification of multiplexed pathogens via a two-step dual-channel fluorescence turn-on array","authors":"Xingliang Huang ,&nbsp;Yang Yu ,&nbsp;Huihai Li ,&nbsp;Zhongzheng Xiong ,&nbsp;Jinwu Wei ,&nbsp;Dengqiong Yu ,&nbsp;Hengfei Zhao ,&nbsp;Liya Liao ,&nbsp;Yong Li ,&nbsp;Chao Guo ,&nbsp;Chao Xu ,&nbsp;Weiwei Ni ,&nbsp;Jinsong Han ,&nbsp;Xuejuan Gao ,&nbsp;Hui Huang","doi":"10.1016/j.aca.2025.344012","DOIUrl":"10.1016/j.aca.2025.344012","url":null,"abstract":"<div><div>Bacterial infections have been an increasingly serious threat to human health. However, the rapid identification of multiplexed bacteria remains challenging due to their intricate composition. Herein, we developed a two-step, dual-channel fluorescence “turn-on” sensor array that sequentially amplifies signals <em>via</em> Indicator Displacement Analysis (IDA) and Aggregation-Induced Emission (AIE). Three weakly fluorescent, positively charged conjugated fluorophores (<strong>A1</strong>–<strong>A3</strong>) with AIE properties were designed to form electrostatic complexes (<strong>C1</strong>–<strong>C3</strong>) with negatively charged graphene oxide (<strong>GO</strong>). Upon addition of bacteria, fluorophores were released from the electrostatic complexes <em>via</em> IDA, resulting in fluorescence turn-on. These fluorophores then aggregated on the bacterial surface, further enhancing fluorescence. This array accurately differentiated among 10 distinct bacterial strains, achieving 98.3 % classification accuracy within 30 s. Finally, the approach facilitated semi-quantitative bacterial analysis, multiplex identification, and robust differentiation in artificial urine samples, presenting a promising method for early infectious disease diagnosis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 344012"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}