Analytica Chimica Acta最新文献

{"title":"Development of a portable SERS tool to evaluate the effectiveness of washing methods to remove pesticide residue from fruit surface","authors":"Lydia Freund ,&nbsp;Jacqueline Mossa ,&nbsp;Huiyuan Guo","doi":"10.1016/j.aca.2024.343476","DOIUrl":"10.1016/j.aca.2024.343476","url":null,"abstract":"<div><h3>Background</h3><div>Pesticides are widely used in agriculture to control pests and enhance crop yields. However, post-harvest, there are growing concerns about the potential health risks posed by pesticide residues on produce surfaces. Analyzing these residues is challenging due to their typically low concentrations and the potential interference from the complex matrix of the produce's surface. The problem addressed in this study is the need for a sensitive, rapid, and on-site capable method to detect and quantify pesticide residues on agricultural products.</div></div><div><h3>Results</h3><div>We developed a portable surface-enhanced Raman spectrometer (SERS)-based approach that offers a rapid 10-min turnaround, simplified protocol, on-site capability, and high sensitivity. Using the new analytical method, we evaluated pesticide residues on fruit surfaces after household or industrial postharvest washing, specifically the efficacy in removing the fungicide ferbam from peach surfaces. The limit of detection (LOD) for our method was determined to be 0.012 mg/kg, significantly lower than the U.S. Environmental Protection Agency's regulated limit of 7 mg/kg for ferbam on peaches. Our data shows that soaking in tap water for 1 min is the least effective method for removing ferbam, with insignificant difference from the control group. In contrast, soaking in a vinegar-water or NaHCO₃-water solution for 5 min, as well as in a sodium hypochlorite solution (12 % available chlorine) for 1 or 5 min, proved to be the most effective methods. Extended soaking improved pesticide removal for tap water, vinegar, and NaHCO₃, while in the chlorine groups, the effect was insignificant. SERS analysis revealed negligible penetration of ferbam into peach flesh and the inner surface of the skin.</div></div><div><h3>Significance</h3><div>This study introduces an innovative method for measuring pesticide residues, significantly enhancing our understanding of pesticide removal and penetration. This new analytical approach is crucial for effectively detecting pesticides and mitigating their exposure through food sources.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1336 ","pages":"Article 343476"},"PeriodicalIF":5.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanozymes featuring a mesoporous silica shell for rapid enrichment and ultrasensitive lateral flow immunoassay of influenza A","authors":"Jing Liang ,&nbsp;Xiaoxian Liu ,&nbsp;Zhenzhen Liu ,&nbsp;Xuanming Xu ,&nbsp;Yinuo Sun ,&nbsp;Yuan Chen ,&nbsp;Rui Xiao ,&nbsp;Yajie Wang","doi":"10.1016/j.aca.2024.343474","DOIUrl":"10.1016/j.aca.2024.343474","url":null,"abstract":"<div><h3>Background</h3><div>Respiratory illnesses stemming from influenza A viruses represent a significant worldwide health concern. There is an immediate need for a rapid and sensitive method to detect influenza A viruses early, without requiring extra equipment.</div></div><div><h3>Results</h3><div>Here, we established a lateral flow immunoassay (LFIA) for the detection of influenza A (Flu A) using a \"three-in-one\" multifunctional mesoporous Fe₃O₄@SiO₂@Pt nanozymes (Fe₃O₄@MSiO₂@Pt NZs) with excellent magnetic separation properties, colorimetric, and peroxidase-like (POD-like) activities. Effective enrichment of target Flu A in complex samples as well as greater loading of Pt particles by mesoporous structures with large specific surface area to enhance POD-like activity can significantly improve the detection sensitivity of the LFIA. After colorimetric amplification by Fe₃O₄@MSiO₂@Pt tags catalysis, the qualitative and quantitative results of detection for Flu A nucleoprotein (Flu A-NP) were 0.01 and 0.0089 ng mL⁻¹, respectively. This indicated a sensitivity approximately 100 times greater than commercially available colloidal Au nanoparticle (AuNP)-based LFIA strips. For detection of inactivated H1N1 virus, quantification can be as low as 33 copies mL⁻¹. Moreover, it demonstrated high accuracy in pharyngeal swab sample simulation experiments.</div></div><div><h3>Significance</h3><div>Therefore, the proposed platform based on Fe₃O₄@MSiO₂@Pt NZs-LFIA offered a promising approach for point-of-care testing (POCT), enabling rapid and ultrasensitive diagnosis of Flu A.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1335 ","pages":"Article 343474"},"PeriodicalIF":5.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic drug monitoring of methotrexate by disposable SPCE biosensor for personalized medicine","authors":"Hui Liu ,&nbsp;Yi-Wei Liu ,&nbsp;Ruo-Yu Yang ,&nbsp;Mei-Juan Wu ,&nbsp;Zi-Wei Yu ,&nbsp;Jing-Wen Han ,&nbsp;Chen-Zhi Zhang ,&nbsp;Pin-Fang Huang ,&nbsp;Ai-Lin Liu ,&nbsp;Meng-Meng Liu","doi":"10.1016/j.aca.2024.343473","DOIUrl":"10.1016/j.aca.2024.343473","url":null,"abstract":"<div><h3>Background</h3><div>Methotrexate (MTX) is widely used in clinical practice for the treatment of malignant tumors and autoimmune diseases. High-dose MTX has been shown to be an effective approach for treating various malignant tumors, but it is accompanied by numerous toxic side effects, necessitating therapeutic drug monitoring (TDM) for patients and timely “folinic acid rescue.” High-performance liquid chromatography and fluorescent immunoassay (FIA) are currently used to detect MTX, but these methods are limited by complex sample preparation, time consumption, and high cost. Therefore, a simple, rapid, and cost-effective MTX measurement method is required.</div></div><div><h3>Results</h3><div>We developed a flexible and inexpensive electrochemical sensor using a stearyl trimethyl ammonium bromide (STAB)-modified, screen-printed carbon electrode to directly detect MTX in human serum. Assay performance was validated via detection of MTX in spiked buffer. The sensor was capable of measuring MTX concentrations ranging from 0.01 to 1 μM and 1–1500 μM, with a limit of detection of 3.1 nM and a limit of quantitation of 3.5 nM. For the samples simulating combined medication, the sensor exhibited outstanding selectivity, in cross-reactivity, with the maximum response value of interferents reaching only 3.49 %. Additionally, the sensor shows reliable repeatability with a relative standard deviation of 3.8 % and remarkable stability. Recovery in human serum validated the clinical utility of the sensor in point-of-care testing conditions. The sensor's applicability to personal medicine was confirmed by detecting MTX blood concentration in patients with different diseases. The results obtained by the sensor were compared with those obtained by the FIA technique, a method commonly used in hospitals, showing a high level of consistency between both methods.</div></div><div><h3>Significance</h3><div>To meet the requirements of personalized medicine for MTX-patients, we developed a disposable biosensor with wide detection range from 0.01 to 1 μM and 1–1500 μM. Owing to the effective enrichment of STAB, the electrochemical response was sensitive, selective, stable, and rapid. As per the clinical test results, our sensor has shown a high level of consistency with the FIA method, indicating its potential to replace FIA as a cost-effective platform for MTX-TDM.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1335 ","pages":"Article 343473"},"PeriodicalIF":5.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances and opportunities of hydrogel-based artificial olfactory colorimetricsystems for food safety detection: A review","authors":"Helei Yang, Zhaopeng Li, Jinfu Zhang, Zhenhe Wang, Hua Zhou, Pei Li, Xia Sun","doi":"10.1016/j.aca.2024.343431","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343431","url":null,"abstract":"<h3>Background</h3>Food safety has attracted increasing attention in recent years. Harmful gases often produced during food storage have devastating effects on human health and ecosystems, and identifying and detecting them is essential. To date, many traditional methods have been used to monitor the freshness of food products. These methods have high precision and sensitivity, but their application to daily life is limited because they are time-consuming and require complex instruments. Therefore, the development of simple, rapid, and low-cost methods for evaluating food freshness is essential.<h3>Results</h3>Hydrogel-based colorimetric artificial olfactory systems have attracted a great deal of attention and have been widely applied in the field of food safety testing in recent years. The system has the advantages of a low detection limit, portability, onsite monitoring, high precision and rapid detection. This superiority makes the colorimetric hydrogel gas sensor a strong contender for improving food safety detection. This paper reviews the application of a hydrogel-based colorimetric artificial olfactory system for detecting hazardous gases for food safety purposes. The main sections of this review succinctly discuss the construction principles and operational mechanisms of the colorimetric artificial olfactory system. Additionally, it delves into the selection and preparation methodologies of hydrogel materials. This paper focuses on advancements in hydrogel-based colorimetric artificial olfactory systems for detecting gases, such as ammonia, biogenic amines, and H₂S that are relevant to food safety. Furthermore, this work outlines the challenges encountered by this artificial olfactory system and proposes potential solutions to overcome them.<h3>Significance and Novelty</h3>The study presented in this paper demonstrates the potential application of a hydrogel-based colorimetric artificial olfactory system for food safety detection, and complements the previous review of colorimetric hydrogel gas sensors for the detection of spoilage gases in food products and serves as a reference for further advanced research and applications in this field.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"11 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of microRNA-21 based on smartly designed ratiometric electrochemical sensor and dual-signal amplification","authors":"Honglei Shao, Xingming Xue, Zhaomei Sun, Xiangjiang Zheng, Pengfei Shi","doi":"10.1016/j.aca.2024.343444","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343444","url":null,"abstract":"MicroRNA(miRNA) serves as an effective and viable biomarker for early diagnosis and monitoring of cancer disorders. It is highly expressed in tumor cells, including lung cancer, liver cancer and lymphoma. Herein, we propose a ratiometric electrochemical sensor for ultrasensitive detection of miRNA-21 using dual signal amplification, hybridization chain reaction and Exo III assisted-amplification. Methylene blue (MB) and Hemin are chosen as two electrochemical species. Then the ratiometric electrochemical sensor were developed, which showed favorable performance of miRNA-21 detection, and exhibited a detection concentration range from 1 fM to 10 nM. Notably, the limit of detection for this biosensor was 0.15 fM. Overall, this strategy for miRNA detection holds significant promise for early cancer screening.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"61 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrroloquinoline quinone exhibiting peroxidase-mimicking property for colorimetric assays","authors":"Ning Xia ,&nbsp;Fengli Gao ,&nbsp;Gang Liu ,&nbsp;Yong Chang ,&nbsp;Lin Liu","doi":"10.1016/j.aca.2024.343468","DOIUrl":"10.1016/j.aca.2024.343468","url":null,"abstract":"<div><h3>Background</h3><div>Small molecule mimics offer the advantages of easy preparation, good thermodynamic stability, and reproducible catalytic activity. However, most of the reported organic artificial mimics face challenges including low catalytic activity, oxidative self-destruction, and auto-aggregation into inactive dimers. Therefore, novel organic mimics with high catalytic activity as well as good thermal and environmental stability are highly desirable.</div></div><div><h3>Results</h3><div>We found that pyrroloquinoline quinone (PQQ), a nutritionally important growth factor, displayed significant peroxidase-like activity to catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidized TMB (oxTMB) by H₂O₂. Its catalytic activity surpassed that of other organic small molecules reported previously, including fluorescein derivatives and adenosine/guanosine triphosphate. Based on the reduction of oxTMB by the enzymatically generated ascorbic acid (AA), the PQQ/TMB-based system was used to monitor alkaline phosphatase (ALP) activity with ascorbic acid 2−phosphate (AAP) as the substrate. This sensing system allowed for the determination of ALP with a detection limit of 1 mU/mL. Furthermore, we discovered that PQQ can coordinate with Cu²⁺ to form metal-organic nanoparticles. The building blocks of two active cofactors endowed the nanoparticles with impressive functionalities for biocatalytic applications. With Cu-PQQ nanoparticles as signal labels, colorimetric immunoassays of prostate specific antigen (PSA) were achieved with a lowest detectable concentration down to 0.1 pg/mL.</div></div><div><h3>Significance</h3><div>This work is valuable for the design of novel biosensors by utilizing PQQ as an artificial enzyme because of its high catalytic activity, good stability, ease of storage, and simple chemical structure.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1335 ","pages":"Article 343468"},"PeriodicalIF":5.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic optimizing surface-enhanced Raman scattering (SERS) strategy of detection and in-situ monitoring of photodegradation of Benzo[a]pyrene in water","authors":"Qinyi Li,&nbsp;Qiangting Zheng,&nbsp;Jiangli Shi,&nbsp;Yu Yan,&nbsp;Xiaoyu Guo,&nbsp;Haifeng Yang","doi":"10.1016/j.aca.2024.343466","DOIUrl":"10.1016/j.aca.2024.343466","url":null,"abstract":"<div><h3>Background</h3><div>Polycyclic aromatic hydrocarbons (PAHs) are one of the most dangerous persistent organic pollutants in the environment. Due to the discharge of chemical plants and domestic water, the existence of PAHs in sea water and lake water is harmful to human health. A method for rapid detection and removal of PAHs in water needs to be developed.</div></div><div><h3>Results</h3><div>In this work, we fabricate Fe₃O₄ wrapped with gold nanoparticles decorated graphene oxide composite nanomaterial (Fe₃O₄/GO/Au). Excellent magnetism of Fe₃O₄ provides a means to enrich and separate the PAHs from substrates, which effectively improve the selectivity and avoid the coffee ring effect in traditional SERS detection. Fe₃O₄/GO/Au shows great response to PAHs owing to the π-π electronic interaction between PAHs and GO, and through the calculation of Material Studio, it is confirmed that GO has different adsorption capacity for polycyclic aromatic hydrocarbons with different number of benzene rings. The limit of detection (LOD) of Benzo[a]pyrene (BaP) can reach 3.8 μg⋅L⁻¹ and other PAHs can also be detected at μg⋅L⁻¹ level. Gold nanoparticles demonstrate prominent light absorption within the visible spectrum and are characterized by their localized surface plasmon resonance (LSPR) properties. Meanwhile, Au NPs facilitate electron migration and carrier separation of GO. Owing to the synergistic interplay, the composite system Fe₃O₄/GO/Au exhibits the capability for SERS sensitive detection and photocatalytic degradation of BaP under visible light irradiation, realizing in-situ monitor the photodegradation of BaP in water.</div></div><div><h3>Significance and novelty</h3><div>A rapid and accurate quantitative method is established for the detection and removal of PAHs in water, which is intended to provide new strategy for the quantitative analysis and removal of water environmental pollution.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1336 ","pages":"Article 343466"},"PeriodicalIF":5.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-pass cavity-enhanced Raman spectroscopy of complex natural gas components","authors":"Miaolin Wang ,&nbsp;Jianxin Wang ,&nbsp;Pinyi Wang ,&nbsp;Ziyi Wang ,&nbsp;Sirui Tang ,&nbsp;Guochao Qian ,&nbsp;Tanglong Liu ,&nbsp;Weigen Chen","doi":"10.1016/j.aca.2024.343463","DOIUrl":"10.1016/j.aca.2024.343463","url":null,"abstract":"<div><h3>Background</h3><div>The concentration of natural gas components significantly impacts the transportation, storage, and utilization of natural gas. Consequently, implementing online monitoring and leak detection systems is vital to guarantee the efficient use of natural gas and to uphold its safe and stable operation. Raman spectroscopy offers distinctive benefits, including high selectivity, superior precision, and the capability to detect multiple gas components simultaneously using a single-wavelength laser. Nevertheless, the inherent weakness of the Raman effect in gases results in limited detection sensitivity for Raman spectroscopy, which may not suffice for specific practical applications.</div></div><div><h3>Results</h3><div>This paper presents a study investigating the detection of natural gas's complex components using a high-sensitivity multi-cavity enhanced Raman spectroscopy technique. An enhanced folded Z-shaped multi-pass cavity has been constructed to amplify the interaction length between the laser and the gas, thereby significantly boosting the Raman signal intensity by 1000 times. The detection limits for CH₄, C₂H₆, C₃H₈, <em>n</em>-C₄H₁₀, <em>i</em>-C₄H₁₀, <em>n</em>-C₅H₁₂, <em>i</em>-C₅H₁₂, and <em>n</em>-C₆H₁₄ gases reached 0.12, 0.53, 0.55, 0.67, 0.28, 0.46, 0.34, and 0.71 ppm, respectively. The least squares method was utilized to establish quantitative relationships between the characteristic peak heights and the concentrations of gases, encompassing both single-component and mixed multi-component systems. Additionally, the natural gas samples were configured and subsequently detected and analyzed.</div></div><div><h3>Significance</h3><div>As proposed in this paper, the results indicate that the MPC-CERS system boasts several advantages, including a low detection limit, high quantitative accuracy, excellent detection repeatability, and robust system stability. Furthermore, its capability for real-time monitoring is well-suited to meet the gas detection requirements in practical applications. Consequently, the research presented in this paper offers innovative insights for the online tracking and leak detection of distributed energy natural gas systems.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1336 ","pages":"Article 343463"},"PeriodicalIF":5.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobodies’ duo facilitates ultrasensitive serum HER-2/neu immunoassays via enhanced avidity interactions","authors":"Suchanat Boonkaew ,&nbsp;Laura Teodori ,&nbsp;Mikkel H. Vendelbo ,&nbsp;Jørgen Kjems ,&nbsp;Elena E. Ferapontova","doi":"10.1016/j.aca.2024.343472","DOIUrl":"10.1016/j.aca.2024.343472","url":null,"abstract":"<div><h3>Background</h3><div>Existing liquid biopsy assays for protein biomarkers of cancer are mostly based on antibodies (Ab) contributing unfavorably to their high cost. Easy to express and modify in vitro, nanobodies may be a cost-effective alternative to Ab.</div></div><div><h3>Results</h3><div>We show that serum HER-2/<em>neu</em>, a biomarker and target of aggressive HER-2/<em>neu</em>(+) cancers, can be accurately detected in a 1.2 h electrochemical cellulase-linked sandwich nanobody/aptamer assay on magnetic beads. Using a single nanobody receptor, 2Rs15d or 2Rb17c, reduces immunoassay's sensitivity by 35%–26 %. A combination of two nanobodies as a duo-receptor recovers the sensitivity of the enzyme-linked nanobody/aptamer-sorbent assay (ELNASA) to 11.9 ± 2.8 μC fM⁻¹, due to the avidity effects making the nanobodies-duo binding properties comparable to those of Ab. Down to 0.1 fM HER-2/<em>neu</em> was detected by ELNASA in serum samples, with no interference from other blood-circulating proteins. In a 30 healthy-volunteers trial, ELNASA more accurately than optical ELISA assayed serum HER-2/<em>neu</em>.</div></div><div><h3>Significance</h3><div>ELNASA performance rivals that of ELISA, yet estimated to be at least 200 times cheaper, due to the lower cost of nanobodies production, and may be better suited for routine clinical analysis of HER-2/<em>neu</em>, particularly, in low- and middle-income settings with limited resources. The ELNASA approach is generic and may be adapted for specific and ultrasensitive analysis of other blood-circulating proteins.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1335 ","pages":"Article 343472"},"PeriodicalIF":5.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volumetric absorptive microsampling meets electromembrane extraction for the first time: Case example of doxorubicin and its metabolite in whole blood samples","authors":"Adam Reguli ,&nbsp;Hana Bavlovič Piskáčková ,&nbsp;Olga Lenčová-Popelová ,&nbsp;Petra Kollárová-Brázdová ,&nbsp;Martin Štěrba ,&nbsp;Stig Pedersen-Bjergaard ,&nbsp;Petra Štěrbová-Kovaříková","doi":"10.1016/j.aca.2024.343459","DOIUrl":"10.1016/j.aca.2024.343459","url":null,"abstract":"<div><h3>Background</h3><div>Microsampling of biological fluids followed by innovative sample pre-treatment reflects trends in bioanalytical chemistry. Volumetric absorptive microsampling (VAMS) enables exact whole blood volume collection and reduces the impact of hematocrit on the assay. In animal studies, it complies with the 3R principles (refine, reduce, replace). It allows for a gentle bleeding technique and a reduction in the number of laboratory animals by enabling ethically acceptable repeated blood collection from a single animal. Treating VAMS tips with electromembrane extraction (EME) in 96-well format offers a smart combination of non-invasive, low-volume blood collection with effective, environmentally friendly sample clean-up.</div></div><div><h3>Results</h3><div>This study introduces the first application of EME in 96-well format for direct isolation of analytes from 10 μL of whole blood collected onto a VAMS device. Doxorubicin, a clinically used anticancer drug also utilized in cancer/cardio-oncology research involving rodents, where microsampling offers important advantages, and its metabolite doxorubicinol, were selected as relevant analytes. The optimized EME yielded reproducible recoveries for both analytes regardless of hematocrit levels, different anticoagulants, or free multivalent ions in the sample. Compared to conventional VAMS tips treatment, EME reduced matrix effects, increased throughput, and an environmental friendliness of the extraction. The EME followed by the UHPLC-MS/MS assay was validated for both analytes in whole blood absorbed onto VAMS tips. The same protocol was implemented to treat plasma to determine the blood-to-plasma ratio of the analytes in the same experiments. The practical utility was demonstrated by analyzing real samples collected from the doxorubicin-treated nude mice.</div></div><div><h3>Significance</h3><div>The study offers a novel assay combining whole blood microsampling and sample clean-up in microextraction scale for preclinical pharmacokinetic studies with doxorubicin in rodents and for pharmacokinetic/pharmacodynamic modeling. This advancement in bioanalytical chemistry promotes scalable environmentally friendly procedures compatible with the 3R ethical principles in animal studies. Moreover, the concept of direct VAMS tips treatment with EME may also be easily translatable to clinical settings.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1335 ","pages":"Article 343459"},"PeriodicalIF":5.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}