Analytica Chimica Acta最新文献

{"title":"Pyrene functionalized organic cation receptor-based “turn-on” fluorescence approach for monitoring of chlorpyrifos in food, soil, and water samples","authors":"Manish Kumar ,&nbsp;Aman Dhiman ,&nbsp;Gagandeep Singh ,&nbsp;Navneet Kaur ,&nbsp;Narinder Singh","doi":"10.1016/j.aca.2024.343488","DOIUrl":"10.1016/j.aca.2024.343488","url":null,"abstract":"<div><h3>Background</h3><div>The unregulated use of pesticides by farmers, for crop productivity results in widespread contamination of organophosphates in real environmental samples, which is a growing societal concern about their potential health effects. The conventional approaches for the monitoring these organophosphate-based pesticides which include immunoassays, electrochemical methods, immunosensors, various chromatography techniques, along with some spectroscopic methods, are either costly, sophisticated, or involves the use of different metal complexes. Therefore, there is an urgent need for sensitive, quick, and easy-to-use detection techniques for the screening of widely used organophosphate-based pesticides.</div></div><div><h3>Results</h3><div>Herein, we demonstrates the metal-free detection of CPF pesticide in aqueous medium, based on the organic nanoparticles of benzimidazole-based cationic receptor (<strong>R1</strong>-ONPs), and thoroughly analyzed using advanced techniques such as AFM, FESEM, and DLS etc. The photophysical investigations revealed that developed <strong>R1</strong>-ONPs exhibited high selectivity towards chlorpyrifos with an enhancement in fluorescence emission. Further, the observed pyrene excimer-based \"turn-on\" fluorescence mechanism, and the interaction between developed sensor and chlorpyrifos has been validated utilizing ¹H, and ³¹P NMR spectroscopy. The developed sensor can effectively quantify chlorpyrifos up to a detection limit of 18.9 nM (3<em>σ</em> method) with a range of 0–120 μM as well as below the cutoff limit set by FAO. Moreover, the real-time application of developed sensor (<strong>R1</strong>-ONPs) was evaluated to monitor chlorpyrifos in spiked food, water, and soil samples with good (%) recovery.</div></div><div><h3>Significance</h3><div>The development of metal-free, pyrene-excimer-based “Turn-On” fluorescent sensor offers a novel, eco-friendly strategy for the detection of chlorpyrifos in aqueous medium. Additionally, its ability to quantify the chlorpyrifos at levels as low as those set by FAO makes it more efficient tool for monitoring the environmental toxicity ensuring better protection for human, and animal health.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1336 ","pages":"Article 343488"},"PeriodicalIF":5.7,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modular comparison of untargeted metabolomics processing steps","authors":"Markus Aigensberger, Christoph Bueschl, Ezequias Castillo-Lopez, Sara Ricci, Raul Rivera-Chacon, Qendrim Zebeli, Franz Berthiller, Heidi E. Schwartz-Zimmermann","doi":"10.1016/j.aca.2024.343491","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343491","url":null,"abstract":"<h3>Background</h3>Untargeted metabolomics requires robust and reliable strategies for data processing to extract relevant information form the underlying raw data. Multiple platforms for data processing are available, but the choice of software tool can have an impact on the analysis. This study provides a comprehensive evaluation of four workflows based on commonly used metabolomics software tools: XCMS, Compound Discoverer, MS-DIAL, and MZmine. These tools were applied to a dataset derived from bovine saliva samples spiked with small polar molecules analysed by anion exchange chromatography coupled to high resolution mass spectrometry.<h3>Results</h3>The analysis revealed significant differences in the number and overlap of detected features, with only approximately 8% of the features included in all four peak tables. Among the overlapping features, MS-DIAL demonstrated the greatest similarity to manual integration, while XCMS and MZmine also performed well. In contrast, Compound Discoverer had issues to reliably integrate high baseline peaks. This study also explores various post-processing strategies, including missing value imputation, transformation, scaling, and filtering. The assessment of missing values indicated that they primarily originated from low abundance, making imputation with small values the most effective approach. No clear evidence suggested that transformation is necessary for downstream statistical analyses. Auto-scaling emerged as the most suitable strategy for data scaling. Low thresholds for blank filtering were found to be the most effective in enhancing data quality. The optimization of filtering thresholds required a careful balance to remove unnecessary information while retaining vital data.<h3>Significance and Novelty</h3>This work provides an overview of commonly applied strategies in untargeted metabolomics analysis, emphasizing the importance of careful workflow selection and optimization. It serves as a resource for refining data processing strategies to achieve accurate and reliable results, while also offering fresh insights into the challenges encountered throughout the untargeted metabolomics processing pipeline.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"79 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of five antineoplastic agents in swab samples using UPLC-ESI-MS/MS: Method development and validation","authors":"Eline Verscheure ,&nbsp;Matteo Creta ,&nbsp;Katrien Poels ,&nbsp;Jeroen Vanoirbeek ,&nbsp;Manosij Ghosh ,&nbsp;Lode Godderis","doi":"10.1016/j.aca.2024.343492","DOIUrl":"10.1016/j.aca.2024.343492","url":null,"abstract":"<div><h3>Background</h3><div>Antineoplastic agents are hazardous drugs used in cancer treatment and consequently can be present at the workplace (e.g. hospital), but also in a home-setting in case of treatment at home. Given the serious health effects, it is important to monitor environmental contamination on a regular base. As currently, no method allows for simultaneous quantification of cyclophosphamide, etoposide, mitomycin C, imatinib and 5-fluorouracil, we developed and validated a method for simultaneous quantification of multiple antineoplastic agents in swab samples.</div></div><div><h3>Results</h3><div>Sample collection was optimised and samples were extracted using solely an extraction solvent (water or water/acetonitrile), shaking and sonicating. Ultra performance liquid chromatography coupled to tandem mass spectrometry with electrospray ionisation was used for detection of the compounds. Selectivity, linearity, sensitivity, precision and accuracy were determined. The LLOQs were 0.3 ng/swab (cyclophosphamide, mitomycin C), 1 ng/swab (etoposide) and 5 ng/swab (imatinib, 5-fluorouracil). After a spill, quantifiable concentrations of antineoplastic agents could be measured in swabs using the developed methods. After cleaning, all concentrations fell below the LLOQ.</div></div><div><h3>Significance</h3><div>Two methods allowing for safe collection, rapid preparation and sensitive quantification were developed and can be applied in the field to measure environmental contamination. One for simultaneous quantification of cyclophosphamide, etoposide, mitomycin C and imatinib, one for quantification of 5-fluorouracil.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1336 ","pages":"Article 343492"},"PeriodicalIF":5.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible disk ultramicroelectrode: facile preparation and high-resolution scanning electrochemical microscopy imaging","authors":"Xinfang Zhang, Xuanhao Mei, JinPeng Bao, Ping Song, Haiyan Lu, Ce Han, Weilin Xu","doi":"10.1016/j.aca.2024.343490","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343490","url":null,"abstract":"<h3>Background</h3>Scanning electrochemical microscopy (SECM) is a kind of scanning probe technology that enables the obtainment of surface morphology and electrochemical information by recording changes in Faraday current triggered by the movement of probe.<h3>Results</h3>In this work, flexible disk ultramicroelectrode (UME) with highly repeatable geometry are fabricated through a simple and universal strategy that involves vacuum pulling the glass capillaries inserted with platinum wire (gold wire, carbon fiber, etc.), followed by a rapidly heated sealing and polishing process. Based on the optical and electrochemical characterization, the as-prepared UMEs possess favorable geometric shape with a small RG value from 1.7 to 2. Consequently, when applied as probe components for SECM imaging on interdigital electrodes, it can achieve excellent spatial resolution and high sensitivity at short working distances. Moreover, due to the outstanding flexibility, the as-prepared UMEs can be minimally damaged arising from tip-sample collision and produce invisible trace on apple peel after the contact scanning, which is far superior to the traditional UME and suggests the application potential in bio-related fields.<h3>Significance</h3>Our work will inspire the application of flexible UMEs as probe components in SECM, and consequently promote the widespread use of SECM with reduced collision threshold and low operational difficulty.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"198 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable paper-based microfluidic devices with Cu1-xAgxS NPs modification for multiplex intelligent visualized detection of adrenaline and glucose simultaneously","authors":"Huan Wang ,&nbsp;Lihua Dong ,&nbsp;Gina Ma ,&nbsp;Shuang Qiu ,&nbsp;Guiye Shan ,&nbsp;Lijia Zhao ,&nbsp;Yu Sun ,&nbsp;Anni Cui ,&nbsp;Rui Zhang ,&nbsp;Xiaojie Liu","doi":"10.1016/j.aca.2024.343489","DOIUrl":"10.1016/j.aca.2024.343489","url":null,"abstract":"<div><h3>Background</h3><div>Adrenaline and glucose are essential biomarkers in human body for maintaining metabolic balance. Abnormal levels of adrenaline and glucose are associated with various diseases. Therefore, it is important to design portable, on-site devices for rapid adrenaline and glucose analysis to safeguard health. Traditional paper-based analytical devices (μPADs) for multiplexed detection typically required multiple recognized probes, increasing the cost and complexity. Moreover, non-uniform color distribution, caused by lateral chromatographic elution effect, reduced results accuracy. Hence, it is necessary to construct visualized μPADs with controlled flow capability using single sensing probe for simultaneous adrenaline and glucose analysis in point-of-care testing (POCT).</div></div><div><h3>Results</h3><div>The portable multiplexed 3D-folded μPADs have been developed for the visualized, accurate and simultaneous detection of adrenaline and glucose, employing Ag-doped copper sulfide nanoparticles (Cu_1-xAg_xS NPs) as multifunctional sensing probe. The μPADs integrated the sampling entrance layer, buffer layer and test layer into a single device through wax printing, allowing the analyte solution to flow vertically from buffer zones to detection zones, thereby minimizing lateral chromatographic elution effect. Cu_1-xAg_xS NPs, exhibiting laccase-like and peroxidase-like multi-enzyme activities, were modified on various detection zones of μPADs for multiplex colorimetric analysis of adrenaline and glucose. Based on Cu_1-xAg_xS-induced colorimetric reactions, the orange-red and blue colors were generated in adrenaline and glucose detection zones, respectively. Subsequently, color signals were converted into RGB values via smartphone application (APP) for rapid and intelligent results analysis. The Cu_1-xAg_xS-incorporated μPADs exhibited colorimetric uniformity and accuracy with limit of detection (LOD) of 10.2 nM and 11.5 μM, respectively.</div></div><div><h3>Significance</h3><div>The adjustable laccase-like and peroxidase-like activities of Cu_1-xAg_xS NPs induced by Ag doping provide a new perspective for multiple biomarkers analysis. Benefiting from the excellent multienzyme activity of Cu_1-xAg_xS NPs, μPADs can perform on-site, visual and multiplex analysis using a single probe, demonstrating their potential for POCT in personal healthcare. The 3D folded Cu_1-xAg_xS-incorporated μPADs with integrated horizontal and vertical flows exhibit uniform color signals for obtaining accurate detection results.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1336 ","pages":"Article 343489"},"PeriodicalIF":5.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serially coupled columns enhance rapid separation and predictive interaction understanding of 93 fentanyl analogs","authors":"Jiyu Kim,&nbsp;Sumin Seo,&nbsp;Chohee Jeong,&nbsp;Eunbin Bae,&nbsp;Donghee Lee,&nbsp;Juhyeon Kim,&nbsp;Eunjin Ko,&nbsp;Hamin Choi,&nbsp;Sang Beom Han","doi":"10.1016/j.aca.2024.343479","DOIUrl":"10.1016/j.aca.2024.343479","url":null,"abstract":"<div><h3>Background</h3><div>The simplicity of synthesis methods has facilitated the illegal manufacture of various fentanyl analogs, leading to numerous fatal overdoses worldwide, particularly in North America. Fentanyl analogs with similar structures are difficult to distinguish due to their fragmentation patterns, making separation using chromatography essential. Additionally, because fentanyl analogs are lethal even in trace amounts, they are easily smuggled, and commonly used fentanyl test strips often fail to detect them due to their low sensitivity. Therefore, the urgent need for analytical methods that can simultaneously identify multiple analogs and swiftly detect them at low concentrations.</div></div><div><h3>Results</h3><div>In this study, liquid chromatography-tandem mass spectrometry was conducted to screen 93 types of fentanyl analogs among the illegal fentanyl substances. The phenyl-hexyl columns enhance fentanyl analog separation through strong π–π interactions. The serially coupled column system increased the separation efficiency and mitigated peak distortion, particularly those of polar fentanyl analogs. The selectivity varied significantly, depending on the interactions with the combined columns. The phenyl-hexyl column's superior ability to predict fentanyl analog interactions based on molecular structure was confirmed by retention factor analysis. The resolution of fentanyl isomers increased significantly when methanol was used instead of acetonitrile as an organic modifier in the mobile phase. The approach was validated by determining the limits of detection and quantification, specificity, detection capability, recovery, and relative ion intensity.</div></div><div><h3>Significance</h3><div>The fentanyl analogs, including 23 sets of isomeric and isobaric compounds, were analyzed via separation using a phenyl-hexyl column serially coupled with a cyano column. The serially coupled column system increased the separation efficiency and mitigated peak distortion, particularly those of polar fentanyl analogs. The proposed strategy can be adopted in exploring methods of effectively separating mixtures with diverse properties, aiding the prevention of drug abuse and bolstering public health and safety efforts.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1336 ","pages":"Article 343479"},"PeriodicalIF":5.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142712954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep eutectic solution elution assisted ligand affinity assay: A useful tool for the active coumarins screening from Fructus cnidii","authors":"Jianing Su,&nbsp;Bing Li,&nbsp;Kun Yao,&nbsp;Cheng Tang","doi":"10.1016/j.aca.2024.343481","DOIUrl":"10.1016/j.aca.2024.343481","url":null,"abstract":"<div><h3>Background</h3><div>Many of the ligand affinity analyses are presented in water environment, and the hydrophilic solution such as methanol is used for dissociating the bound compounds. The obtained dissociation solution needs to be concentrated for improving the sensitivity of the assay. However, it is not good for the analysis of hydrophobic and volatile compounds such as coumarins. The solubility of them in water environment is low. And they may be lost in the sample preparation process. In this work, a deep eutectic solvent elution assisted ligand affinity assay for the screening of active coumarins in plants was proposed.</div></div><div><h3>Results</h3><div>For the first time, deep eutectic solvent was used as a dissociation solution for dissociating the compounds that bind with acetylcholinesterase immobilized magnetic beads. Analytes could be enriched during the phase separation process of deep eutectic solvent. The concentration of the analytes in sample solution was therefore increased. It is good for the detection of coumarins. The LOD and LOQ of the proposed assay were 0.50 (μ g/mL) and 1.50 (μ g/mL), respectively. The relative recovery of the assay was 99.4 ± 7.2 %. The intra-day and inter-day precision were 7.25 % and 6.80 %. Using the assay as a tool, the active coumarins in Fructus cnidii were analyzed. And two coumarins with acetylcholinesterase inhibition activity such as imperatorin and osthole were found.</div></div><div><h3>Significance</h3><div>Coumarins have good solubility in the selected deep eutectic solvent, and could be enriched into hydrophobic phase during the phase separation process. The extra concentration process is not needed. Compared with traditional methods, the proposed method has the benefits of simplified operation process, high sensitivity, and is friendly to the volatile and hydrophobic compounds.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1336 ","pages":"Article 343481"},"PeriodicalIF":5.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142712750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a new spectral database of photo-induced pesticide compounds for an automatic monitoring and identification system","authors":"Coumba Faye ,&nbsp;El Hadji T. Bodian ,&nbsp;Diène D. Thiaré ,&nbsp;Diéry Diouf ,&nbsp;Ndeye A. Diop ,&nbsp;Jean-Pierre Bakhoum ,&nbsp;Lamine Cisse ,&nbsp;Pape A. Diaw ,&nbsp;Atanasse Coly ,&nbsp;Philippe Giamarchi","doi":"10.1016/j.aca.2024.343475","DOIUrl":"10.1016/j.aca.2024.343475","url":null,"abstract":"<div><h3>Background</h3><div>Due to the widespread use of pesticides, it is necessary to monitor surface water quality for environmental protection, industrial use and tap water production. Many analytical methods based on chromatographic separations and mass spectrometry detection can be used, but they are complex and expensive. They also require sampling and transport to the laboratory, which delays the results. We therefore need automatic monitoring systems that can detect and identify pesticides at low cost and with simple technology.</div></div><div><h3>Results</h3><div>As many pesticides are non-fluorescent, we have chosen the photoinduced fluorescence method for detection. However this method lacks of specificity. To improve this, we present a new fluorescence database of photoinduced pesticide compounds. It is designed in an original way to be lightweight, containing only four spectra of each pesticide concatenated into a single 'combined fluorescence spectrum'. It can therefore be used with a simple spreadsheet to identify pesticides by spectral similarity. The database is then combined with an automated pesticide monitoring system using photo-induced fluorescence to add identification capability.</div><div>Analytical applications are carried out on-line to determine rimsulfuron and sulfometuron-methyl, with good analytical results. The identification process using the database is then self-tested against the spectra of these two pesticides, leading to correct results.</div></div><div><h3>Significance</h3><div>This work presents the first spectrofluorimetric database of photoinduced compounds of pesticides. It has been designed to be easy to use, even by an unskilled user. Combined with an automated monitoring system, it becomes the first to allow simultaneous detection and identification of pesticides in surface water. The results showed good identification results and good analytical performance.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1335 ","pages":"Article 343475"},"PeriodicalIF":5.7,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutathione-functionalized covalent organic frameworks@silica as a hydrophilic-hydrophobic balanced mixed-mode stationary phase for highly efficient separation of compounds with a wide range of polarity","authors":"Yaming Sun ,&nbsp;Wenjie Sun ,&nbsp;Junqi Wang ,&nbsp;Chenchen Song ,&nbsp;Yongxing Hu ,&nbsp;Renyong Zhao ,&nbsp;Wenjie Zhao ,&nbsp;Lijun He","doi":"10.1016/j.aca.2024.343477","DOIUrl":"10.1016/j.aca.2024.343477","url":null,"abstract":"<div><h3>Background</h3><div>Covalent organic frameworks (COFs) are a highly promising stationary phase for high-performance liquid chromatography (HPLC), but the separation of polar compounds is limited by their low hydrophilicity. Therefore, it is crucial to develop novel COFs-based stationary phases with balanced hydrophilicity-hydrophobicity for the efficient separation of different polar compounds.</div></div><div><h3>Results</h3><div>In this paper, glutathione (GSH)-functionalized COFs@silica microspheres (GSH-COFs@SiO₂) were synthesized <em>via</em> a two-step, post-synthesis modification strategy. The COFs particles was constructed onto silica surface by the covalent conjugation of 1,3,5-tris(4-aminophenyl)benzene and 2,5-divinylterephthalaldehyde. GSH containing abundant –NH₂ and –COOH groups was bonded onto the surface of COFs@SiO₂ to further enhance hydrophilicity. The resulting GSH-COFs@SiO₂ exhibited balanced hydrophilicity-hydrophobicity and can be used in hydrophilic/reversed-phase liquid chromatography modes through multiple retention mechanisms. Consequently, a variety of compounds with different polarity, including nucleosides/bases, benzoic acids, anilines, phenols, alkylbenzenes and polycyclic aromatic hydrocarbons, were well separated with ideal resolution, satisfactory column efficiency and good peak shapes. Furthermore, this novel column exhibited remarkable column stability, as evidenced by intra-day relative standard deviations of 0.08 %–0.18 % for retention time and 0.45 %–1.47 % for peak area.</div></div><div><h3>Significance and novelty</h3><div>This work demonstrates the superior hydrophilic-hydrophobic selectivity of GSH-COFs@SiO₂ stationary phases towards compounds with a wide range of polarity and provides a very facile and easily popularized post-synthetic modification route for hydrophilic-hydrophobic balanced COFs-based HPLC stationary phases.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1335 ","pages":"Article 343477"},"PeriodicalIF":5.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Au@Ag hollow nanoshells-based SERS integrated microfluidic chip as a sample-to-answer platform for the ultra-sensitive detection of geosmin","authors":"Yuting Gong ,&nbsp;Dong Li ,&nbsp;Min Chen ,&nbsp;Anhui Lin ,&nbsp;Quansheng Chen ,&nbsp;Xiaomei Chen","doi":"10.1016/j.aca.2024.343471","DOIUrl":"10.1016/j.aca.2024.343471","url":null,"abstract":"<div><h3>Background</h3><div>Geosmin (GSM) can compromise the immune systems of aquatic organisms, rendering them more vulnerable to viral and bacterial infections, thereby adversely affecting their growth, reproduction, yield, and quality. Given the relatively low odor thresholds of GSM, there is a critical demand for the development of a highly sensitive and rapid detection method. According to the principle of Surface-enhanced Raman spectroscopy (SERS), localized surface plasmon resonance (LSPR) greatly enhances the Raman signals of adsorbed molecules. To date, no study has reported the application of SERS to detect GSM.</div></div><div><h3>Results</h3><div>Dual-metal nanomaterials with hollow structures have been proven to provide a large surface area and heightened localized surface plasmon resonance, thereby enhancing the sensitivity of Raman signals. In this study, a sample-to-answer platform was constructed by integrating Au@Ag hollow nanoshells (HNSs)-based SERS and a microfluidic chip for the sensitive, fast, and direct determination of GSM. Under 532 nm excitation, GSM exhibit Raman peaks on the SERS-active Au@Ag HNSs, and there is a relationship between the peak intensity and the concentration of GSM. Owing to the integration of the microfluidic chip, only microliters of reagent are required, and the test results can be achieved within 4 min. The constructed sample-to-answer platform showed a good linear response to GSM in the range of 1 ng/L–1 mg/L, with a detection limit of 0.16 ng/L. An optimal calibration model is established by combining stoichiometric algorithms.</div></div><div><h3>Significance</h3><div>This work realized the ultra-highly sensitive and fast determination of GSM based on SERS-active Au@Ag HNSs, which provides new insights and is very promising for the on-site monitoring of earthy odors. Our study makes a significant contribution to the literature because the developed SERS-based detection method does not require labels or biomaterials, providing the possibility of highly sensitive and on-site testing of contaminants in food and the environment.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1335 ","pages":"Article 343471"},"PeriodicalIF":5.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}