Analytica Chimica Acta最新文献

{"title":"Advanced design of target-driven self-powered sensor assisted by cascade catalytic strategy","authors":"Zongshan Zhang ,&nbsp;Jing Xu ,&nbsp;Lei Zhang ,&nbsp;Gaoli Zhang ,&nbsp;Hui Li","doi":"10.1016/j.aca.2024.343359","DOIUrl":"10.1016/j.aca.2024.343359","url":null,"abstract":"<div><div>In this work, a self-powered microsensor platform based on enzyme biofuel cells (EBFCs) was developed for intelligent monitoring of disease markers miRNA-451. The cascade catalysis system constructed by using the strategy of enzyme-like ZIF-8 nanocapsule incorporation with biological enzymes, which could simultaneously take into account the specificity of biological enzymes and the high activity of nano-enzymes, significantly promoted the electron transfer between glucose and the bio-anode surface, and improved the sensitivity and stability of the sensing system. Meanwhile, the target-triggered hybridization chain reaction (HCR) amplification strategy to achieve exponential signal amplification based on accurate recognition, and jointly improve the detection sensitivity. As expected, the micro-sensor platform has a wide linear range of 0.5–1.0 fmol/L with a low limit of detection (LOD) of 0.13 fmol/L (S/N = 3) and exhibits excellent selectivity, reproducibility and stability in interference assays under optimal detection conditions. The designed self-powered system is simple to construct, easy to transport and the data transmission mode is intelligent and controllable, which is expected to be used in basic biochemical research, clinical diagnosis and environmental monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343359"},"PeriodicalIF":5.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142487309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive targeted profiling of multiple steroid classes in rodent plasma using liquid chromatography-mass spectrometry","authors":"Taťána Gazárková ,&nbsp;Hana Kočová Vlčková ,&nbsp;Kateřina Plachká ,&nbsp;Karla Vagnerová ,&nbsp;Dominika Dubecová ,&nbsp;Petra Klusoňová ,&nbsp;Jiří Pácha ,&nbsp;Frantisek Svec ,&nbsp;Lucie Nováková","doi":"10.1016/j.aca.2024.343362","DOIUrl":"10.1016/j.aca.2024.343362","url":null,"abstract":"<div><h3>Background</h3><div>Reliable quantification of multiple steroid classes in biological fluids within a single method remains an analytical challenge despite many previously published methods. Crosstalk of positional isomers, overlap of stereoisomer fragmentation patterns, differing proton affinities, in-source fragmentation, varying stability of protonated ions in the gas phase across steroid classes, and non-existence of steroid-free matrix are the main challenges limiting the number of simultaneously profiled steroids.</div></div><div><h3>Results</h3><div>In this study, we focused on the development of a derivatization-free, achiral, high-throughput, and cost-effective UHPLC-MS/MS approach that allows simultaneous profiling of a spectrum of 38 steroids covering progestogens, androgens, corticosteroids, and estrogens, while properly addressing the hurdles of steroid analysis. Within a 20-min method, 16 stereoisomers and 15 positional isomers were fully resolved within a single run while separated from 7 additional non-interfering steroids and matrix interferences in rodent plasma. Protein precipitation (PP) and supported liquid extraction (SLE) methods using only 40 μL of sample were developed to achieve the lowest possible limits of quantification. Nevertheless, 5α-dihydroprogesterone and 3α,5α-THDOC could be only qualitatively assessed when using PP. In contrast, DHEA-S could not be quantified or identified when using SLE. A novel surrogate matrix-background subtraction approach, using rat plasma after the animal's adrenalectomy, has been implemented into the optimized PP-UHPLC-MS/MS workflow, successfully validated according to the unified ICH/EMA M10 guidelines, and compared to the traditional quantification strategies. Moreover, the validity of the newly adopted approach has been verified by the targeted profiling of multiple biologically active endogenous steroids in more than 500 samples of mouse plasma in total.</div></div><div><h3>Significance</h3><div>Underestimation of hurdles associated with steroid analysis often compromises the accurate steroid quantification. Our comprehensive, fully validated UHPLC-MS/MS method targeting a wide spectrum of endogenous steroids, mitigating steroid crosstalk and using a minimal sample volume together with a novel surrogate matrix-background subtraction approach significantly advances steroid analysis for research and clinical applications covering multiple biological scopes.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343362"},"PeriodicalIF":5.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142487421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode sensing platform for Bisphenol A detection via bifunctional CsPbBr3@Cu-MOF assisted fluorescence and colorimetric analysis","authors":"Junyi Ma ,&nbsp;Ying-zhuo Shen ,&nbsp;Jianxiao Gong ,&nbsp;Seitkhan Azat ,&nbsp;Zheng Ping Ji ,&nbsp;Xiao-Ya Hu ,&nbsp;Qin Xu","doi":"10.1016/j.aca.2024.343354","DOIUrl":"10.1016/j.aca.2024.343354","url":null,"abstract":"<div><h3>Background</h3><div>Bisphenol A (BPA) has been identified as an endocrine disruptor with numerous detrimental effects on human health. There is an urgent need to develop fluorescence/colorimetric dual-mode sensing approaches with expanded detection linear range, increased accuracy, and enhanced application flexibility for BPA detection. The utilization of fluorescence and colorimetric signals in point-of-care applications and real-time sensitive sensing further highlights the significance of developing novel and efficient fluorescence/colorimetric dual-mode sensing platform with high-efficiency probes.</div></div><div><h3>Results</h3><div>Herein, a fluorescence and colorimetric dual-mode aptasensor was developed by using CsPbBr₃@Cu-MOF as the aptamer immobilization matrix and signal generator. CsPbBr₃ was functionalized with Cu-MOF using a simple two-step strategy. This strategy involved in-situ formation and modified ligand-assisted precipitation technique with 4,4′-bipyridine (4,4-Bpy) serving as the bifunctional linker. The resulting CsPbBr₃@Cu-MOF exhibited improved stability in water and enhanced fluorescence. Additionally, it functioned as peroxidase mimetic to oxidize 3,3′,5,5′-tetramethyl benzidine (TMB), leading to a colorimetric change from colorless to blue. In the presence of BPA, aptamers were removed from CsPbBr₃@Cu-MOF. Consequently, the fluorescence and peroxidase-activity of CsPbBr₃@Cu-MOF were recovered, resulting in the enhanced fluorescence intensity and color change of TMB. Using this system, the proposed aptasensor demonstrated detection ranges of 1.0–80.0 nM with a LOD of 0.60 nM for the colorimetric method, and a linearity range of 0.1–100 nM with a LOD of 0.02 nM for the fluorescence method.</div></div><div><h3>Significance</h3><div>The obtained CsPbBr₃@Cu-MOF composites showed excellent fluorescence properties, good peroxidase-like activity, and aqueous stability. Furthermore, the proposed dual-mode aptasensor demonstrated simplicity, cost-effectiveness and good anti-interference abilities. This can be extended to the construction of other dual-mode sensors by changing aptamers and provides novel insights on the potential applications of perovskites in bioanalysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343354"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced data point importance: Layered significance of variables in multivariate calibration","authors":"Somaye Vali Zade ,&nbsp;Klaus Neymeyr ,&nbsp;Mathias Sawall ,&nbsp;Hamid Abdollahi","doi":"10.1016/j.aca.2024.343357","DOIUrl":"10.1016/j.aca.2024.343357","url":null,"abstract":"<div><h3>Background</h3><div>The Enhanced Data Point Importance (EDPI) method, a systematic approach for evaluating the importance of data points in multivariate calibration, is introduced. Factor decomposition methods allow for the evaluation of the impact of variables on maintaining the structural pattern of data in the abstract space. Essential data points play a key role in these patterns and the method of Data Point Importance (DPI) aims to evaluate the essential data points in terms of their importance. All other points are rated by zero. In this contribution, DPI is extended to include inner points to evaluate the importance of these points in the absence of the essential points. The EDPI method employs convex peeling to sort data points systematically.</div></div><div><h3>Results</h3><div>EDPI method was applied to near-infrared and Raman spectroscopy data sets, including corn and alcohol mixtures and simulated data, to rank and select important variables. EDPI effectively identified variables that contributed to the preservation of the data structure and highlighted key spectral regions with different degrees of selectivity. In the alcohol dataset, EDPI revealed important physicochemical insights by focusing on specific regions where non-analytes spectra overlapped. It performed in a similar way to the Variable Importance in Projection (VIP) method, but with fewer variables selected.</div></div><div><h3>Significance</h3><div>The experimental results obtained from calibrating near-infrared and Raman spectroscopic datasets using partial least squares highlight the effectiveness of the proposed EDPI strategy when contrasted with the conventional variable importance in projection (VIP) method for variable selection.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343357"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-The-Flight trapping, LIBS analysis and discrimination of single meteorite particles generated by laser ablation","authors":"C. Burgos-Palop ,&nbsp;F.J. Fortes ,&nbsp;P. Purohit ,&nbsp;T. Delgado ,&nbsp;J. Laserna","doi":"10.1016/j.aca.2024.343361","DOIUrl":"10.1016/j.aca.2024.343361","url":null,"abstract":"<div><h3>Background</h3><div>Thousands of micrometeorites fall to the Earth on a daily basis. Most of these meteorites have a rocky composition, but others are mainly composed of iron and nickel. Due to their small size, often ca. 100 μm in diameter, the process of searching for, collecting, and identifying these samples is remarkably tedious. In this work, we introduce a minimally invasive methodology for evaluating the full elemental composition of micrometeorites using optical emission spectroscopy of single particles produced by laser ablation of bulk targets.</div></div><div><h3>Results</h3><div>Bulk meteorite samples were directly ablated within an ablation cell. From few micrograms of ablated matrix, we originated dry aerosols consisting of multielemental particles which were representative of the sample chemistry. SEM images confirmed that the generated particles exhibited spherical geometry. Particles were first optically trapped in air and, then, analyzed by laser-induced breakdown spectroscopy (LIBS). LIBS spectra evidenced compositional differences among samples. For example, <em>Campo de Cielo</em> meteorite featured a high iron content due to its metallic nature whereas LIBS results for <em>Jbilet Winselwan</em> and <em>NWA 869</em> suggested that pyroxene components dominated the composition of the samples. In contrast, <em>NWA 13739</em> and <em>Vaca Muerta</em> contained high aluminum intensity, thus indicating that the feldspathic component was dominant, as then verified by XRD. The intra-sample compositional variability were quite satisfactory, as revealed by the RSD data, below 45 %. For quantitative analysis, the percentages of FeO, SiO₂, Al₂O₃, Na₂O, MgO, TiO₂, CaO, K₂O, MnO, SrO, Cr₂O₃, and Li₂O were calculated using CF-LIBS.</div></div><div><h3>Significance</h3><div>This work demonstrates the applicability of laser excitation of individual particles in an optical trap for the multielemental analysis of meteorites. The methodology provides a complete overview of the samples, is capable of classificating them according to their main phases and yield preliminary quantitative information about those phases. Therefore, we present a minimally destructive pathway to be used as the first step in the inspection of these delicate samples. If the particles represent nanostructures inherent to the meteorites, it would constitute a major step in the analysis of extraterrestrial material that may provide fundamental insights into the structure and composition of the original materials at the microscale, a topic that remains an active area of research worldwide.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343361"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mid-level data fusion strategy based on urinary nucleosides SERS spectra and blood CEA levels for enhanced preoperative detection of lymph node metastasis in colorectal cancer","authors":"Jinyong Lin ,&nbsp;Yuduo Wu ,&nbsp;Zhizhong Lin ,&nbsp;Xueliang Lin ,&nbsp;Qiong Wu ,&nbsp;JiaJia Lin ,&nbsp;Yuanji Xu ,&nbsp;Shangyuan Feng ,&nbsp;Junxin Wu","doi":"10.1016/j.aca.2024.343360","DOIUrl":"10.1016/j.aca.2024.343360","url":null,"abstract":"<div><h3>Background</h3><div>Preoperative prediction of lymph node metastasis (LNM) plays a crucial role in the treatment and prognosis of colorectal cancer (CRC). The traditional histopathological examination is invasive and time-consuming, providing pathological features only postoperatively. Preoperative serum carcinoembryonic antigen (CEA) is strongly correlated with postoperative LN status. However, the detection accuracy of LNM based on a single preoperative CEA level is low. Therefore, developing a more powerful and sensitive diagnostic tool would be of great clinical value for improving the accurate preoperative prediction of LNM in CRC patients.</div></div><div><h3>Results</h3><div>This study aimed to develop a mid-level fusion approach using urinary nucleosides Raman spectra and blood CEA data to enhance the preoperative discrimination of CRC patients with and without LNM. Surface-enhanced Raman scattering (SERS) spectra of urinary modified nucleosides, isolated by affinity chromatography, were first acquired from 48 patients with LNM and 49 patients without LNM. The principal component analysis (PCA) scores obtained from the SERS spectra were then combined with preoperative blood CEA values to create a fused data array. The discriminant accuracy based on either dataset alone or the fused data was evaluated using three machine learning algorithms: linear discriminant analysis, k-nearest neighbors, and support vector machine. Results showed that the fused data could discriminate between the two groups with an accuracy of up to 91 %, outperforming SERS alone (86 %) and CEA alone (69 %).</div></div><div><h3>Significance</h3><div>To our knowledge, this is the first report of mid-level data fusion of urinary nucleosides SERS spectra with blood CEA levels for the preoperative prediction of LNM in CRC. This work demonstrates that the mid-level data fusion strategy aided by SVM algorithm can greatly improve the preoperative prediction accuracy of LNM. This is crucial for therapeutic decision-making and prognostic assessment in CRC.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343360"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescence imaging of dopamine in living cells triggered by unique coupling reactions","authors":"Shaofei Fu ,&nbsp;Jianwen Qiu ,&nbsp;Biaofeng Gu ,&nbsp;Yong Gao","doi":"10.1016/j.aca.2024.343358","DOIUrl":"10.1016/j.aca.2024.343358","url":null,"abstract":"<div><h3>Background</h3><div>Dopamine (DA) is a significant neurotransmitter and catecholamine molecule in the mammalian brain. It plays a crucial role in perception, cognition, central nervous system regulation, and hormone secretion. The detection of DA levels in living systems is a vital aspect of the research and early diagnosis of neurological disorders.</div></div><div><h3>Results</h3><div>In this study, we achieved an effective fluorescence generation specific to DA under physiological pH conditions by coupling the DA detection reaction to the HClO oxidation reaction. This method has been used for the detection of DA in cells, as well as for the visualization of DA levels in cellular models of inflammation and depression.</div></div><div><h3>Significance</h3><div>This is the first time that utilizes an organic fluorescent molecular probe to detect DA in living cells.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343358"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a multiplexing method for the quantification of “high-risk” host cell lipases in biotherapeutics by Luminex","authors":"Jeremy D. Osko,&nbsp;Shannon Rivera,&nbsp;Fengqiang Wang,&nbsp;Thomas Niedringhaus","doi":"10.1016/j.aca.2024.343349","DOIUrl":"10.1016/j.aca.2024.343349","url":null,"abstract":"<div><div>Clearance of residual Host Cell Proteins (HCPs) is critical for the manufacturing processes of biotherapeutics. HCPs have the potential to impact product efficacy and quality, posing a risk to patient safety. It is therefore essential to be able to both identify and quantitate HCPs throughout drug development, even if the proteins are present in low concentrations. Traditional Enzyme-Linked Immunosorbent Assays (ELISAs) have historically served as the gold standard for monitoring HCPs; however, ELISA methods are labor-intensive and costly. With an increase of HCPs being identified below detectable quantification levels, there is a need for simultaneous detection of selectively targeted HCPs. Here, we develop a Luminex multiplexing method that is able to accurately quantify two “high-risk” lipases Lipoprotein Lipase (LPL) and Phospholipase B-Like 2 (PLBL2) within the same assay. This study outlines the method development for optimizing parameters such as antibody constructs, conjugation ratios, signal enhancement, and more in order to create the most efficient multiplexing method. As a result, a Luminex multiplexing method can provide a similar result to a monoplexing ELISA method but in a faster and more cost-effective manner. This method can be expanded to include other “high-risk” HCPs and used for future HCP applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343349"},"PeriodicalIF":5.7,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A visual electrochemiluminescence sensor based on the combination of distance-readout strategy and nanozymes catalyzing precipitation","authors":"Jiadong Zhang ,&nbsp;Yanming Yang ,&nbsp;Kexing Zhu ,&nbsp;Yujiang Wang ,&nbsp;Lijing Zhang ,&nbsp;Xiangyu Ma ,&nbsp;Nan Hao ,&nbsp;Liangdong Feng ,&nbsp;Yi Chen","doi":"10.1016/j.aca.2024.343355","DOIUrl":"10.1016/j.aca.2024.343355","url":null,"abstract":"<div><h3>Background</h3><div>Nanozyme is a kind of biomimetic enzyme with unique properties and catalytic function of nanomaterials. Various nanozymes have been applied in the development of visual biosensors because of its excellent stability and availability. Nevertheless, the current applications of nanozymes primarily focus on colorimetric sensing based on color changes, which is susceptible to external factors interference including sample solution color and ambient light. Incorporating a distance-readout strategy with nanozymes presents a promising avenue for the advancement of innovative visual biosensing technologies in the future.</div></div><div><h3>Results</h3><div>A visual electrochemiluminescence (ECL) sensor was developed by combining the distance-readout strategy with nanozymes catalyzing precipitation for the first time. The detection is based on the ECL length change induced by nanozymes. This sensor chip comprises a square detection area and a visual ECL channel area. A peroxidase-mimic, core-shell hollow Fe₃O₄@PDA@ZIF-67, was modified in the detection region. The substrate 4-chloro-1-naphthol was rapidly catalyzed by nanozymes to form purple precipitate benzo-4-chlorohexanedione, leading to an increase in impedance within the detection region. Consequently, the detection area's partial voltage rose while the visual channel's dropped, resulting in a shorter ECL length. As a proof-of-concept experiment, the visual distance-readout ECL sensor was utilized for the quantitative detection of H₂O₂. The alteration in ECL length exhibited a good linear relationship with H₂O₂ concentration (logarithm) within the range of 300 nM to 300 μM, with the limit of detection (LOD) of 87 nM.</div></div><div><h3>Significance</h3><div>This work developed a novel approach for the application of nanozymes in analytical chemistry. Compared with common colorimetric sensing, this method is more convenient and intuitive, which can effectively eliminate potential interference from humans and the environment during signal acquisition. Because biocatalytic precipitation can be formed through multiple sensing processes, this strategy holds great promise as a portable and cost-effective tool with diverse applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343355"},"PeriodicalIF":5.7,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-channel fluorescent probe for detecting SO2 and Hg2+, and dynamic imaging SO2 under Cd2+ in alfalfa (Medicago sativa)","authors":"Ya-Qian Wu ,&nbsp;Xiang-Yu Wang ,&nbsp;Tian-Wen Bai ,&nbsp;Ying-Long Fu","doi":"10.1016/j.aca.2024.343353","DOIUrl":"10.1016/j.aca.2024.343353","url":null,"abstract":"<div><h3>Background</h3><div>As an antioxidant, SO₂ plays a critical role in maintaining the plant's redox balance. At the same time, Hg²⁺ as heavy metals, will accumulate in plants through soil, often disrupting physiological processes in plants. However, few studies have been reported to examine changes in Hg²⁺ and SO₂ in plants. Here, we developed a dual-channel fluorescent probe NYD, which can differentiate between the detection of Hg²⁺ and SO₂.</div></div><div><h3>Results</h3><div>The probe exhibits high sensitivity (LOD, 44 nM for Hg²⁺ and 26 nM for SO₂). When Hg²⁺ and HSO₃⁻ deprotect and attack the double bond by nucleophilic addition, significant emission of fluorescence at 475 nm and 574 nm with blue and red fluorescence respectively. In addition, NYD has the capability to detect the levels of Hg²⁺ and SO₂ in cells and alfalfa seedlings. Meanwhile, the findings indicated that alfalfa seedlings were exposed to Cd²⁺, which led to redox imbalance and increased endogenous SO₂ production.</div></div><div><h3>Significance</h3><div>We anticipate that this research will contribute to a better understanding of the functions of SO₂ under oxidative stress induced by Cd²⁺.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343353"},"PeriodicalIF":5.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142450127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}