Analytica Chimica Acta最新文献

{"title":"Assessment of thermal and solvent stable SPME fibers for volatilome investigation through in vitro experiments with cancer cell lines","authors":"Wojciech Filipiak ,&nbsp;Merve Çakmakçı ,&nbsp;Sılanur Sevgen ,&nbsp;Paulina Szeliska ,&nbsp;Karol Jaroch ,&nbsp;Atakan Kara ,&nbsp;Kübra Kahremanoğlu ,&nbsp;Barbara Bojko ,&nbsp;Ezel Boyaci","doi":"10.1016/j.aca.2025.344708","DOIUrl":"10.1016/j.aca.2025.344708","url":null,"abstract":"<div><h3>Background</h3><div>solid phase microextraction (SPME) was introduced to bioanalysis years ago, but still, it did not find its place in routine analysis, despite the many advantages it offers. The limited number of commercial devices and protocols ready to be adopted for multi-residue analysis, i.e. untargeted metabolomics, are among the most important reasons. In this study, HLB-PTFE-coated SPME fibers were optimized for in vitro cell line studies targeting the volatilome of cancer cells.</div></div><div><h3>Results</h3><div>the HLB-PTFE SPME fibers with 10 mm coating length were prepared using dip coating approach with home-made HLB particles. The fiber sampling optimization and evaluation of its performance was performed in cell line media spiked with 18 representative compounds selected from the previous preliminary study on A549 lung cancer cell line. The results showed that headspace extraction for 20 min at 38 °C followed by 2 min desorption at 230 °C to GC-MS provides a reliable method with detection limits in a range of 2.2–43 ng/mL and repeatability better than 10% for determination of the volatiles in headspace of cell-line media. Moreover, the application of the developed method in B16F10 melanoma and LL2 lung cell lines revealed successful differentiation based on volatilome profiling.</div></div><div><h3>Significance</h3><div>the satisfactory results of optimization studies and accordance of results obtained here for cell line applications and literature indicate that the method developed with HLB-PTFE-coated SPME fibers and GC-MS approach suit future IVIVE study and meeting 3Rs rules requirements.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344708"},"PeriodicalIF":6.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective molecular interplay in electrochemical sensing: DNA-templated Hg(II) capture and enzyme-driven SeO42− reduction for mercury removal","authors":"Zixin Yu ,&nbsp;Meissam Noroozifar ,&nbsp;Kagan Kerman ,&nbsp;Heinz-Bernhard Kraatz","doi":"10.1016/j.aca.2025.344699","DOIUrl":"10.1016/j.aca.2025.344699","url":null,"abstract":"<div><div>Understanding and controlling selective molecular interactions at bioelectronic interfaces are crucial for developing high-performance electrochemical sensors. In this work, we present a DNA-enzyme functionalized gold electrode for the dual-mode detection and precipitation of mercury (Hg(II)). The sensor utilizes thymine-rich DNA strands to selectively capture Hg(II), forming stable T-Hg(II)-T complexes, while an immobilized enzyme catalyzes the selective reduction of selenate (SeO₄²⁻) to selenite (SeO₃²⁻). The in situ-generated SeO₃²⁻ reacts with the bound Hg(II), triggering the precipitation of HgSeO₃ and facilitating the controlled release of mercury from the DNA scaffold. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) revealed distinct charge transfer resistance (R_CT) variations, corresponding to Hg(II) binding, SeO₄²⁻ reduction, and HgSeO₃ precipitation. The sensor exhibited exceptional selectivity, a detection limit in the sub-nanomolar range, and strong anti-interference performance from competing metal ions and oxyanions. Environmental sample testing further validated its applicability, showing high recovery rates (90.3–109.8 %). The results showed excellent agreement with the inductively coupled plasma mass spectrometry (ICP-MS) measurements as a conventional Hg(II) detection method. This work advances DNA-enzyme-mediated electrochemical sensing by integrating molecular recognition with an intrinsic detoxification mechanism. The findings provided a promising foundation for the next-generation bioelectronic platforms, enabling real-time monitoring of both inorganic mercury and selenate and a potential mercury removal method.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344699"},"PeriodicalIF":6.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-modal fusion model combines SERS spectroscopy and clinicopathological features to predict neoadjuvant therapy response in breast cancer","authors":"Murong Li ,&nbsp;Fei Xie ,&nbsp;Longfei Yin ,&nbsp;Xiongwei Cai ,&nbsp;Wenting Yu ,&nbsp;Zhaonan You ,&nbsp;Tiantian Liu ,&nbsp;Lei Chen ,&nbsp;Song Yu ,&nbsp;Guohua Wu ,&nbsp;Shu Wang","doi":"10.1016/j.aca.2025.344677","DOIUrl":"10.1016/j.aca.2025.344677","url":null,"abstract":"<div><div>Breast cancer remains a significant global health threat to women, with neoadjuvant therapy (NAT) playing a critical role in treatment. Early prediction of NAT efficacy is essential for personalizing therapy and improving patient outcomes. The Miller-Payne (MP) grading system is a widely accepted standard for evaluating treatment response, categorizing patients as non-major histologic responders (MP1∼MP3) or major histologic responders (MP4∼MP5). This study developed a multi-modal fusion model integrating clinicopathological features and pre-treatment serum surface-enhanced Raman spectroscopy (SERS) data to predict NAT response in breast cancer patients. Leveraging Principal Component Analysis (PCA) for spectral dimensionality reduction and a Transformer architecture for feature extraction, the model achieved an accuracy of 92.6 % on the training cohort, significantly outperforming single-modal models using only SERS or clinicopathological features. Double-blind validation on an independent cohort confirmed the model's generalizability with an accuracy of 90 % and an area under the receiver operating characteristic curve (AUC) of 93 %. SERS analysis revealed significant spectral differences related to uric acid, tryptophan, phospholipids, and collagen, which have potential as biomarkers for NAT efficacy prediction. This study innovatively combined serum SERS data with clinicopathological features to predict NAT response in breast cancer patients. The multi-modal fusion model, enhanced by PCA and a Transformer architecture, captured biomolecular and clinical information, improving prediction accuracy and robustness. This non-invasive, cost-effective tool enables clinicians to avoid ineffective NAT, optimize treatment strategies, and improve patient outcomes.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344677"},"PeriodicalIF":6.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FALCON-Net: A hybrid fuzzy-attentive LSTM-convolutional neural network architecture for high-precision classification of steel alloys via femtosecond laser-ablation spark-induced breakdown spectroscopy","authors":"Zhenman Gao,&nbsp;Jianyong Zhuang,&nbsp;Xiaoyong He","doi":"10.1016/j.aca.2025.344701","DOIUrl":"10.1016/j.aca.2025.344701","url":null,"abstract":"<div><h3>Background</h3><div>Steel alloy classification demands advanced methodologies to address spectral noise, matrix effects, and nonlinear interactions in laser-induced breakdown spectroscopy (LIBS). This study proposes FALCON-Net, a hybrid architecture integrating fuzzy logic, LSTM, attention mechanisms, and CNN to enhance LIBS-based identification. Utilizing femtosecond laser-ablation spark-induced breakdown spectroscopy (fs-LA-SIBS), the model processes spectral data from nine steel alloys with distinct Cr, Mn, Ni, Cu, V, Mo, and Al compositions. The fuzzy layer employs Gaussian functions to suppress noise, while attention-guided LSTM captures temporal dynamics and critical wavelengths. CNN extracts spatial features, and fused representations enable robust classification.</div></div><div><h3>Results</h3><div>FALCON-Net achieves 100 % accuracy across four test sets, with ROC curves yielding perfect AUC scores (1.0), surpassing benchmarks (RF: 97.89 %, SVM: 97.23 %). Under 20 dB Gaussian noise, it maintains 97.76 % accuracy, validating noise resilience. Meanwhile, the feature fusion technique reduces wavelength interference and improves model stability.</div></div><div><h3>Significance</h3><div>This work presents the first integration of fuzzy logic and attention-enhanced deep learning for LIBS-based steel alloy analysis. The exceptional performance of FALCON-Net highlights the transformative potential of hybrid architectures in addressing real-world spectroscopic challenges. By offering high accuracy and noise resilience, the framework provides a viable solution for industrial quality control and material characterization, accelerating the practical implementation of fs-LA-SIBS technique in precision metallurgy.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344701"},"PeriodicalIF":6.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of single-cell heterogeneity and invasive potential in cancer cells via secreted protease activity assay","authors":"Benjamin T. Schelske,&nbsp;Ethan H. Leung,&nbsp;Joseph T. Banovetz,&nbsp;Jared L. Anderson,&nbsp;Robbyn K. Anand","doi":"10.1016/j.aca.2025.344663","DOIUrl":"10.1016/j.aca.2025.344663","url":null,"abstract":"<div><h3>Background</h3><div>This paper presents an assay for the secreted protease activity of single cancer cells isolated within chambers of a microfluidic array. The methodology builds on the dielectrophoresis and bipolar electrode (DEP-BPE) platform, enabling live-cell secretion analysis following selective, label-free DEP-based cell isolation. <strong>While many existing assays rely on population-level measurements or labeling strategies, there remains a critical need for label-free, functional tools that capture heterogeneity in live-cell secretion behavior at the single-cell level</strong>.</div></div><div><h3>Results</h3><div>The reported workflow allows for quantification of protease secretion behavior among individual cells, acquisition of temporal secretion profiles, and identification of highly invasive cells. Specifically, we assess the matrix metalloprotease 9 (MMP9) secretion of isolated cancer cells and report substantial heterogeneity in both secreted MMP9 concentration and secretion dynamics at the single-cell level. A linear fluorescence response (R² = 0.98) enables quantification down to 3.31 nM MMP9 (5.4 × 10⁵ molecules) in 280 pL volumes. In a mixed population, 34 of 132 cells (26 %) exhibited MMP9 secretion above background, and 14 cells secreted more than any untreated control cell, revealing a highly invasive subpopulation obscured in ensemble measurements.</div></div><div><h3>Significance</h3><div>This platform enables functional single-cell analysis of invasive potential, providing insights into cellular heterogeneity that are critical for evaluating disease progression and treatment response. The novelty of this work lies in its integration of single-cell isolation by DEP, which is selective for cell type, with a functional assay for a secreted enzyme. The approach is generalizable to selective isolation and molecular assay of many cell types.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344663"},"PeriodicalIF":6.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Charge-regulated reduction potential of phenazine enabling electrochemiluminescence for monitoring riboflavin in beans germination","authors":"Hao-Tian Zhu ,&nbsp;Mi Chen ,&nbsp;Ai-Jun Wang ,&nbsp;Pei-Xin Yuan ,&nbsp;Jiu-Ju Feng","doi":"10.1016/j.aca.2025.344700","DOIUrl":"10.1016/j.aca.2025.344700","url":null,"abstract":"<div><div>Nowadays, organic molecules with excellent fluorescence often lack electrochemiluminescence (ECL), which restricts their analytical and biomedical applications. In this work, we functionalized phenazine (PZ) derivatives with the charged moieties and activated their ECL properties using K₂S₂O₈ as a coreactant, achieving a significant “off-to-on” ECL emission transition. For that, 7,8-dihydroxyphenazine-2-carboxylic acid (DHPZ) was synthetized, showing the largest ECL efficiency of 56.4 % (versus Ru(bpy)₃²⁺ as a standard). Its subsequent self-assembly with ethylene glycol (termed DHPZ-EG) as emitter further boosted the 4.0-fold ECL efficiency. In the presence of riboflavin (RF), the DHPZ-EG assemblies underwent light-induced photocatalytic oxidation (PR), enabling efficient ECL signal transduction <em>via</em> the structure-property correlation. The as-established ECL sensor exhibited a wide linear section from 10 nM to 1.0 mM, a limit of detection (LOD, 1.5 nM), and the ability to monitor broad bean growth metabolism. This work opens a novel avenue for designing organic luminophores and developing sensors for dynamic botanical tracking.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344700"},"PeriodicalIF":6.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-emissive Ru@UiO-66-NH2 composite for selective visual detection of phosphate and arsenate","authors":"Qian Wang ,&nbsp;Chao Wang ,&nbsp;Lili Xie ,&nbsp;Hong Yu ,&nbsp;Ying Wang ,&nbsp;Xian-En Zhao ,&nbsp;Shuyun Zhu ,&nbsp;Renjun Wang","doi":"10.1016/j.aca.2025.344704","DOIUrl":"10.1016/j.aca.2025.344704","url":null,"abstract":"<div><h3>Background</h3><div>The existence of excess phosphate (Pi) and arsenate ions [As(V)] in environment water poses significant risks to ecosystem as well as public health and often interfere each other during detection process. Although some ratiometric sensors exhibited high sensitivity towards individual Pi and As(V), few of them were applied for visual detection and portable sensing. Therefore, we need to design a convenient strategy for selective visual detection of Pi and As(V).</div></div><div><h3>Results</h3><div>Dual-emissive zirconium-based metal-organic frameworks (UiO-66-NH₂) encapsulated tris(2,2-bipyridyl)ruthenium (II) chloride hexahydrate [Ru(bpy)₃²⁺] composite, denoted as Ru@UiO-66-NH₂, has been synthesized by one-pot approach and used for ratiometric fluorescence detection of Pi and As(V). Under single excitation of 295 nm, the composite exhibited two emissions at 435 and 618 nm, originating from UiO-66-NH₂ and Ru(bpy)₃²⁺, respectively. Upon the addition of Pi (or As(V)), the ligand-to-metal charge transfer (LMCT) process was weakened owing to their strong affinities with Zr–O node, inducing the enhanced blue fluorescence and unchangeable red one. In addition, they triggered clear fluorescence changes from red to pink and finally to blue. With the assistance of smartphone, visual detection of Pi and As(V) can be achieved with a limit of detection (LOD) of 0.8 μM and 1.5 μM, respectively. Furthermore, Pi and As(V) could be separately detected via the introduction of proper masking agents.</div></div><div><h3>Significance</h3><div>The detection method developed in this work has successfully been applied to detect Pi and As(V) in actual water samples. For the first time, Ru@UiO-66-NH₂ was used for the visual detection of Pi and As(V). This work broadens the latent applications of MOF composites for on-site monitoring in environment safety assessment.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344704"},"PeriodicalIF":6.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A crossed hollow electrode point discharge for highly efficient vapor sample introduction and spectral excitation","authors":"Qingsong Tang ,&nbsp;Aixiang Chen ,&nbsp;Meng Zhang ,&nbsp;Kai Li ,&nbsp;Xiandeng Hou ,&nbsp;Xiaoming Jiang","doi":"10.1016/j.aca.2025.344705","DOIUrl":"10.1016/j.aca.2025.344705","url":null,"abstract":"<div><h3>Background</h3><div>Discharge microplasma has been popularly utilized as the crucial component of excitation source for miniaturized optical emission spectrometer. However, its overall excitation capability and efficiency are still limited due to the reduction of size and power, thus limiting its wide field analytical applications.</div></div><div><h3>Results</h3><div>A new compact microplasma excitation source was constructed by using a crossed hollow electrode point discharge for a miniaturized optical emission spectrometer, with achieving both highly efficient vapor sample introduction and spectral excitation. Two pairs of electrodes were arranged crossly in a coplanar mode, and one of the four electrodes was with a hollow structure. In the discharge chamber, the plasma region was overlapped and greatly enlarged, which improved the overall excitation capability. Meanwhile, the hollow electrode was also used as the introduction tube for sample vapor; thus, all the gaseous analyte could be confined into the plasma region for full excitation and improved excitation efficiency. Through coupling with hydride generation for vapor sample introduction, under the selected conditions, As, Ge, Hg, Pb, Sb, Se, and Sn could be efficiently excited for optical emission detection, with limits of detection of 1.8, 0.7, 0.1, 0.4, 0.8, 6.2, and 0.1 μg L⁻¹, respectively, and with relative standard deviations all less than 4 % (<em>n</em> = 5). Compared to conventional point discharge microplasma with a single pair electrodes, the analytical sensitivities for the test elements were improved by 3–5 times. Certified Reference Materials were successfully analyzed with this miniature device to demonstrate its accuracy and availability.</div></div><div><h3>Significance</h3><div>This study provided an effective strategy to improve the excitation capability and efficiency of point discharge microplasma source for high-performance miniaturized optical emission spectrometric instruments. It features additional advantages of compactness, low power consumption and simple operation, thus showing potential prospects in fast field elemental analysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344705"},"PeriodicalIF":6.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A combination strategy of HPLC and boric acid modified β-CD polymer for super-efficiently separating chiral substances with O/N atoms in chiral and/or adjacent carbons","authors":"Xiaoqian Yuan ,&nbsp;Yuchen Cui ,&nbsp;Yaqin Nan ,&nbsp;Ruijun Tang ,&nbsp;Xinru Wang ,&nbsp;Yanmei Xu ,&nbsp;Xinghua Jin ,&nbsp;Yiwen Wang ,&nbsp;Ding Cheng ,&nbsp;Youxin Li","doi":"10.1016/j.aca.2025.344687","DOIUrl":"10.1016/j.aca.2025.344687","url":null,"abstract":"<div><h3>Background</h3><div>Cyclodextrins (CDs) and their derivatives have been widely used as chiral selectors in HPLC. However, many derivatized CDs lack specificity for certain drugs and often exhibit limited resolution. Furthermore, their synthesis typically involves complex multi-step procedures, hindering scalability and cost-efficiency. To overcome these limitations, this study aims to develop a simple and scalable boric acid-modified β-cyclodextrin polymer (BA-β-CDP), targeting chiral substances with O/N atoms in chiral and/or adjacent carbons.</div></div><div><h3>Results</h3><div>BA-β-CDP was synthesized on a large scale using inexpensive reagents and a random substitution strategy, avoiding tedious protection-purification steps. Structural and chromatographic compatibility was confirmed by nine characterization techniques. In HPLC using the chiral mobile-phase additive (CMPA) method, BA-β-CDP enabled the efficient separation of 25 racemates. Among them, 24 achieved complete or baseline separation, and 10 exhibited resolution (Rs) values &gt; 10. Typical cases included bisoprolol (Rs = 48.19), propranolol (Rs = 45.50), chlorpheniramine (Rs = 38.36), and metoprolol (Rs = 20.26). Additionally, the material demonstrated excellent batch-to-batch reproducibility, recyclability, and reusability.</div></div><div><h3>Significance and novelty</h3><div>This work presents a practical and cost-effective chiral selector. The synergistic mechanism offers strong and specific recognition toward chiral substances with O/N atoms in chiral and/or adjacent carbons. This approach represents a novel strategy for efficient and economical HPLC-based enantioseparation using widely accessible materials and methods.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344687"},"PeriodicalIF":6.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-channel self-amplifying lateral flow immunoassay for the on-site ultrasensitive simultaneous detection of salivary cortisol and melatonin","authors":"Seonjong Kim ,&nbsp;Hee-Ju Kang ,&nbsp;Jae-Min Kim ,&nbsp;Min-Gon Kim","doi":"10.1016/j.aca.2025.344697","DOIUrl":"10.1016/j.aca.2025.344697","url":null,"abstract":"<div><div>The on-site simultaneous measurement of cortisol and melatonin is valuable for comprehensively assessing circadian rhythms and understanding their complex interplay under physiological conditions. However, no commercial point-of-care test currently enables the concurrent detection of these salivary hormones. Consequently, there is a significant demand for an on-site, user-friendly diagnostic platform. While the lateral flow immunoassay (LFI) is widely used in point-of-care testing, its sensitivity is insufficient for detecting salivary cortisol and melatonin. To overcome this limitation, the self-amplifying LFI (saLFI) developed previously for salivary cortisol detection, is extended to enable salivary melatonin detection in combination with a gold enhancement method. Two saLFIs are integrated into a single device using a dual-channel sample pad, creating a dual-channel self-amplifying lateral flow immunoassay (2ch-saLFI). This integrated dual-channel sample pad provides an equal distribution of the sample solution to two strips and two self-amplifying complexes. Consequently, the novel 2ch-saLFI enables the simultaneous on-site detection of both hormones from a single sample injection, delivering results within 30 min. The 2ch-saLFI demonstrates a high sensitivity, with detection limits of 5.39 and 0.476 pg mL⁻¹ for cortisol and melatonin, respectively (i.e., below typical physiological levels). Validation using 20 clinical samples provides a strong correlation with standard ELISA methods (R² = 0.9017 for cortisol and 0.9101 for melatonin). Overall, the 2ch-saLFI represents the first point-of-care test enabling the on-site, simultaneous, and highly sensitive measurement of salivary cortisol and melatonin via a single sample injection. This development allows an affordable clinical assessment of circadian biology for advanced healthcare applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1378 ","pages":"Article 344697"},"PeriodicalIF":6.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145127933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}