Analytica Chimica Acta最新文献

{"title":"Determination of low-level 90Sr in large volumes of seawater","authors":"Miao Fang ,&nbsp;Lingxia Yao ,&nbsp;Jing Zhang ,&nbsp;Xiaolin Hou","doi":"10.1016/j.aca.2025.343936","DOIUrl":"10.1016/j.aca.2025.343936","url":null,"abstract":"<div><div>The release of radioactive substances into the environment, particularly the discharge of contaminated water from the Fukushima Daiichi Nuclear Power Plant, has raised public concern. ⁹⁰Sr, a highly hazardous radionuclide, remains a significant challenge for accurate determination in environmental seawater due to its low concentration. In this work, an effective co-precipitation of ⁹⁰Sr with SrCO₃ and CaCO₃ was established for pre-concentration of ⁹⁰Sr from a large volume of seawater up to 45 L by using an appropriate concentration of (NH₄)₂CO₃ instead of Na₂CO₃ followed by removal of calcium by hydroxide precipitation. A chemical yield of (88 ± 2)% was achieved for strontium from 45 L of seawater, and the decontamination factors for most radionuclides were higher than 10⁴. The ⁹⁰Y ingrown from ⁹⁰Sr decay was used for the determination of ⁹⁰Sr, and sulfate precipitation was employed to remove radionuclides of Sr, Ba, Ra, and Pb, and the Y was further purified by hydroxide precipitation. The chemical yield of Y was higher than 90% in this step, and the counting efficiency of ⁹⁰Y by liquid scintillation counting reached 100%. The detection limit for ⁹⁰Sr was estimated at 0.11 mBq/L for 45 L seawater, an order of magnitude lower than surface seawater levels without direct human nuclear contamination. The method was validated by the standard addition method and successfully applied to determine ⁹⁰Sr in seawater collected from China seas. The developed method is simple and cost-effective compared to the reported methods and robust for routine analysis of seawater for ⁹⁰Sr.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343936"},"PeriodicalIF":5.7,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143608491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aggregation-induced ECL strategy based on CuAg nanoclusters/Curdlan-g-PGTMAC for gastric cancer detection","authors":"Runze Xu,&nbsp;Ning Liu,&nbsp;Zhenrun Li,&nbsp;Qiang Ma","doi":"10.1016/j.aca.2025.343930","DOIUrl":"10.1016/j.aca.2025.343930","url":null,"abstract":"<div><div>In this work, a novel biosensor based on aggregation-induced luminescence (AIE) of copper-silver alloy nanoclusters (CuAg NCs) has been developed for the detection of gastric cancer marker miRNA-142-3p. The alloy nanoclusters were synthesized by doping Ag ions into Cu NCs as nano-emitters. On the one hand, the doped Ag ions induced Ag–Cu metallophilic interactions, which promoted the relaxation of excited electrons through the radiative pathway. On the other hand, the doped Ag ions can reduce the band gap between the HOMO-LUMO orbitals of the nanoclusters, which decreased the energy consumed for electronic excitation. Therefore, the luminescent signal and stability of CuAg NCs were significantly enhanced. Furthermore, the modification of the permanently positively charged polysaccharide quaternary ammonium salt (Curdlan-g-PGTMAC) on CuAg NCs induced the aggregation-induced electrochemiluminescence (AIECL) effect. The introduction of Curdlan-g-PGTMAC also accelerated the reduction of the co-interaction reagent (S₂O₈²⁻), which significantly improved the ECL generation efficiency. The ECL response of the CuAg NC-based AIECL biosensor showed a good linear correlation with the concentration of miRNA-142-3p over a wide range from 1 fM to 100 nM with a detection limit as low as 8.8 fM. The ECL biosensor with high sensitivity and wide dynamic range was used for the clinical application of miRNA-142-3p in gastric cancer successfully.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343930"},"PeriodicalIF":5.7,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143608202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on precise identification of remote bacterial species using multi-temporal LIBS optimized by plasma electron temperature coefficient of variation","authors":"Jiahui Liang ,&nbsp;Fei Chen ,&nbsp;Zhihui Tian ,&nbsp;Yan Zhang ,&nbsp;Lei Zhang ,&nbsp;Wangbao Yin ,&nbsp;Liantuan Xiao ,&nbsp;Suotang Jia","doi":"10.1016/j.aca.2025.343929","DOIUrl":"10.1016/j.aca.2025.343929","url":null,"abstract":"<div><h3>Background</h3><div>Laser-Induced Breakdown Spectroscopy (LIBS) has demonstrated significant potential in microbial detection due to its rapid, non-contact, and multi-element analytical capabilities. However, remote detection is hindered by challenges such as signal attenuation and the high similarity of spectral features, which reduce classification accuracy. To address these issues, this study proposes a multi-temporal LIBS remote identification method optimized based on the plasma electron temperature coefficient of variation (<em>CV</em>_<em>T</em>). By analyzing <em>CV</em>_<em>T</em> values across different delay ranges, optimal time delays were selected and combined to amplify spectral differentiation, thereby improving classification performance.</div></div><div><h3>Results</h3><div>A coaxial-design LIBS telemetry system with adjustable focus was constructed, and multi-substrate telemetry testing was conducted on 10 common pathogenic bacteria at distances of 5 m and 10 m. By optimizing multiple temporal delays within the 100–1000 ns range, the classification performance of single-temporal, dual-temporal, and multi-temporal spectral combinations was evaluated. The results showed that the multi-temporal approach improved the classification performance across all substrates. At a distance of 5 m, a 100 % identification rate was achieved for all substrates, with Precision, Recall, and F1-score all reaching 1.0. At 10 m, the identification rate for the aluminum substrate increased from 76 % to 93 %. In addition, the contribution of the four major elements, Ca, Na, C, and K, was found to account for up to 60 % of the classification results.</div></div><div><h3>Significance and novelty</h3><div>It is demonstrated that the <em>CV</em>_<em>T</em>-optimized multi-temporal LIBS technology effectively overcomes the signal attenuation bottleneck at long distances, significantly enhancing the robustness and analytical capability of remote microbial identification. This approach provides a novel method for remote detection in areas such as public safety, medical diagnostics, and military defense.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343929"},"PeriodicalIF":5.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143608493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA nanowires-mediated high sensitive quantum dot-fluorescence-linked immunoassay for proteins analysis","authors":"Huanhuan Xing ,&nbsp;Xiaojing Xing ,&nbsp;Fangfang Chen ,&nbsp;Ning Li ,&nbsp;Dangdang Xu ,&nbsp;Ruili Wu ,&nbsp;Yanbing Lv ,&nbsp;Lin Song Li","doi":"10.1016/j.aca.2025.343931","DOIUrl":"10.1016/j.aca.2025.343931","url":null,"abstract":"<div><h3>Background</h3><div>Highly sensitive analysis of protein biomarkers with low concentrations is essential for biological research and medical diagnosis, where quantum dots (QDs) based fluorescence-linked immunoassay (QD-FLISA) has been given considerable attention among the quantitative detection due to its outstanding characteristics. However, the traditional QD-FLISA is usually subject to the low sensitivity owing to the limited photoluminescence (PL) intensity of QDs. In this sense, the development of novel strategy that could remarkably enhance the sensitive of traditional QD-FLISA would be highly desirable.</div></div><div><h3>Results</h3><div>Herein, DNA nanowires-mediated high sensitive QD-FLISA (DNA-nano-QD-FLISA) is first designed and used for the ultrasensitive detection of proteins, where DNA-nanowires are assembled through the hybridization chain reaction (HCR) and C-reactive protein (CRP) is chosen as the model analyte. The results demonstrate that the proposed DNA-nano-QD-FLISA can achieve sensitive detection of CRP, with a limit of detection (LOD) of 0.17 ng/mL, significantly lower than the system without DNA nanowires (1.66 ng/mL). Furthermore, the CRP levels in clinical samples were analyzed, yielding an excellent agreement with the Roche immunoturbidimetric method. Additionally, the versatility of the assay were demonstrated by adapting it to detect the other clinical proteins, interleukin-6 (IL-6) and procalcitonin (PCT), achieving the LODs of 0.07 ng/mL for IL-6 and 0.07 ng/mL for PCT. Furthermore, we found that the length of DNA nanowires significantly influenced the detection performance of QD-FLISA, offering a straightforward approach to precisely adjust the detection range.</div></div><div><h3>Significance</h3><div>This work presents an ultra-sensitive QD-FLISA for protein detection <em>via</em> the introduction of DNA-nanowires assembled through HCR. The achieved results demonstrate that the incorporation of DNA nanowires enhances the detection sensitivity and accuracy of traditional QD-FLISA in quantifying low-abundance biomarkers, which holds significant clinical importance for early disease screening and diagnosis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343931"},"PeriodicalIF":5.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acid-activated bentonite for solid-phase nucleic acid extraction from various pathogenic samples","authors":"Eun Yeong Lee ,&nbsp;Minju Lee ,&nbsp;Myoung Gyu Kim ,&nbsp;Chae Eun Bae ,&nbsp;Sung-Han Kim ,&nbsp;Yong Shin","doi":"10.1016/j.aca.2025.343928","DOIUrl":"10.1016/j.aca.2025.343928","url":null,"abstract":"<div><h3>Background</h3><div>Despite significant advancements in nucleic acid testing technologies, current nucleic acid extraction methods are often limited by inefficiency, complexity, and a lack of versatility. To overcome these challenges, we have developed an innovative solid-phase extraction (SPE) method employing sulfuric acid-activated bentonite (SAB) for extracting nucleic acids from various sample types.</div></div><div><h3>Results</h3><div>Activation with sulfuric acid expands the surface area of bentonite by 2.2 times, thereby enhancing its adsorption capacity and surface modification efficiency. To further improve extraction efficiency, we modified SAB through amine-functionalization using 3-aminopropyl(diethoxy)methylsilane (APDMS), resulting in the creation of APDMS-modified SAB (ASAB). This modification facilitates efficient nucleic acid binding via reversible interactions mediated by a homobifunctional imidoester (HI) reagent. Our ASAB-based SPE system offers a streamlined, universal protocol for isolating DNA, RNA, and miRNA from diverse samples, including clinical bodily fluids and culture media, in under 30 min. Moreover, the system effectively enriches low concentrations of negatively charged pathogens (down to 20 CFU/reaction) from large-volume samples (up to 50 mL) through a 30-min pre-enrichment step utilizing the positively charged ASAB-HI complex. Comparative testing with pooled human urine and plasma samples revealed up to a 3.95-fold increase in DNA recovery compared to commercial SPE kits. Additionally, the system demonstrated up to a 6.3-fold improvement in the isolation of unstable viral RNA from clinical nasopharyngeal swabs, as well as critical microRNA biomarkers.</div></div><div><h3>Significance</h3><div>The versatility and high efficiency of nucleic acid recovery with our ASAB-based SPE system indicate its potential to revolutionize traditional SPE methods, positioning it as a universal nucleic acid extraction platform for molecular biology research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343928"},"PeriodicalIF":5.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical approach to distinguishing lactate dehydrogenase fractions for oncological diagnostics","authors":"Justyna Głowacka-Gudanek,&nbsp;Kamil Gryckiewicz,&nbsp;Kamil Strzelak","doi":"10.1016/j.aca.2025.343921","DOIUrl":"10.1016/j.aca.2025.343921","url":null,"abstract":"<div><h3>Background</h3><div>One of the crucial enzymes for cancer cell growth is lactate dehydrogenase (LDH, E.C. 1.1.1.27), an oxidoreductase that catalyzes the conversion between pyruvate and lactate. It has been found that in cancer cells metabolism, the LDH isoenzyme profile changes, with forms rich in the muscle-type subunit beginning to dominate over those in which the heart-type predominates. This suggests that by examining changes in the enzymatic activity of isoforms with a specific subunit content, it may be possible to quickly distinguish a physiological sample from a pathological one.</div></div><div><h3>Results</h3><div>This article focuses on the development of an analytical strategy that enables the estimation of the ratio of LDH fraction activities as a basis for a simple and quick screening test. Spectrophotometric detection of LDH activity is based on the ferrozine photometric reaction with ferrous ions generated during the biocatalytic reduction of ferric ions by NADH. The developed Multicommutated Flow Analysis (MCFA) system, coupled with an optoelectronic flow-through detector, enables the use of a kinetic method based on the inhibition of LDH subunits to monitor the enzyme reaction kinetics. The distinctly different responses of the muscle-type and heart-type subunits to the selected inhibitors revealed a linear relationship between the obtained analytical signal and the percentage content of each subunit. The calibration curves for selected inhibitors are linear within the tested range of standards with coefficients of determination equal to 0.99 each.</div></div><div><h3>Significance</h3><div>The developed MCFA system was utilized in the analysis of human serum samples obtained from both healthy patients and patients with cancer. The analysis demonstrates that the proposed approach can differentiate oncological serum samples from reference ones based on the LDH fractions activity ratio, even when their total LDH activity level is low.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343921"},"PeriodicalIF":5.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143590170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-functional paper-based analytical device for ultrasensitive detection of peanut allergen-specific IgE","authors":"Ze-Nan Ma ,&nbsp;Jun-Jie Ding ,&nbsp;Xin-Qiao Shi ,&nbsp;Ying Yuan ,&nbsp;Meng-Tian Wang ,&nbsp;Li-Na Yu ,&nbsp;Xiao-Jun Wang ,&nbsp;Peng Shen","doi":"10.1016/j.aca.2025.343922","DOIUrl":"10.1016/j.aca.2025.343922","url":null,"abstract":"<div><h3>Background</h3><div>Increasing attention has been caught by the allergy-related food safety issue. The rapid and sensitive diagnosing approaches are still in high demand for providing clinical reference. Paper-based analytical devices (PADs) are appealing candidates for allergy diagnosis and prediction due to their portability, stability, and operational easiness. However, the sensitivity of PADs needs to be further improved for the targets with low abundance. In addition to the complex signal amplifications, an alternative strategy that requires fewer reagents, steps, and shorter time is anticipated. (82)</div></div><div><h3>Results</h3><div>We report fluorescent PADs (FPADs) that can accumulate and detect the major peanut allergen glycoprotein <em>Arachis hypogaea</em> h2 (Ara h2)-specific IgE (sIgE). The FPADs are constructed by in-situ synthesis of blue-emissive carbon dots (BCDs) on the surface of cellulose paper, followed by the conjugation of Ara h2. After the capture of sIgE, a green-emissive carbon dots-labeled secondary anti-sIgE reporter (Ab2-GCDs) is assembled on FPADs. The detection relies on the sIgE concentration-dependent color variation of FPADs. In addition, the accumulation of sIgE is achievable by filtering the sample through FPADs, improving the assay sensitivity and efficiency. It is demonstrated that the limit of detection (LOD) is 15.7 ng/mL, evidently lower than the simple immersion-based assay (90.2 ng/mL). The excellent selectivity allows sIgE quantification in serum with high accuracy. (130)</div></div><div><h3>Significance</h3><div>By harnessing the outperforming sensing performance of the proposed FPADs, the rapid, accurate, and cost-efficient diagnosis and prediction of peanut allergy can be realized. In addition, the FPADs could serve as a universal sensing platform for varying targets by flexibly engineering the capture moieties on the surface of fluorescent paper. (50)</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343922"},"PeriodicalIF":5.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping human fingerprint beyond level-3 based on an amphiphilic aggregation-induced emission luminogen and the construction of intelligent platform for personal identification","authors":"Xinyi Zhao ,&nbsp;Zixuan Wang ,&nbsp;Haoran Liu ,&nbsp;Siyu Yan ,&nbsp;Zihan Liu ,&nbsp;Yuai Duan ,&nbsp;Tianyu Han ,&nbsp;Tiandong Han","doi":"10.1016/j.aca.2025.343927","DOIUrl":"10.1016/j.aca.2025.343927","url":null,"abstract":"<div><h3>Background</h3><div>Fluorescence imaging agents have been benefiting tremendously from tailor-made aggregation-induced emission (AIE) luminogens, owing to their high on-off ratio, large signal contrast, low background noise as well as the resistance to photobleaching. In the domain of fingerprint imaging, AIE luminogens are beginning to exhibit an advantage owing to the aforementioned superiorities.</div></div><div><h3>Results</h3><div>We present an amphiphilic benzoic-acid salicylaldehyde AIE luminogen <strong>AIE-BASB</strong>, and outline its water sensitivity, self-assembly behavior as well as fingerprint imaging properties. <strong>AIE-BASB</strong> self-assembles into nanoscale textures when fabricated into a drop-casting film but undergoes a disassembly process in response to trace water on fingertip upon physical-contacting. Owing to the biological textures on the skin, fingerprint image can be clearly recorded by <strong>AIE-BASB</strong> film, which reveals detailed microscopic features of fingerprint information ranging from level-1 to level-3. Furthermore, it allows accurate measurements of the sizes, shapes, centroids, and areas of the sweat pores, which leads the fingerprint information into the next level. In addition, we develop an intelligent system based on <strong>AIE-BASB</strong> by integrating hardware and software modules, which is capable of recording and identifying fingerprint. After inputting fingerprint segments in trial operation, this intelligent system makes identification by calculation of the categorical probability, and successfully predicts the classification of the undefined fingerprint segments, implying 100 % accuracy in fingerprint identification.</div></div><div><h3>Significance</h3><div>We predict that <strong>AIE-BASB</strong> may facilitate the development of new biometric technologies, which have broad applications in the domain of artificial intelligence, including machine tactility, target perception and object-machine interaction.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343927"},"PeriodicalIF":5.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143590241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive electrochemical sensor for lipopolysaccharide detection catalyzed by 3,4,9,10-perylenetetracarboxylic diimide","authors":"Wenjie Yu ,&nbsp;Shuaibing Yu ,&nbsp;Fenghong Zhang ,&nbsp;Qinyuan Xu ,&nbsp;Xueji Zhang ,&nbsp;Jinming Kong","doi":"10.1016/j.aca.2025.343926","DOIUrl":"10.1016/j.aca.2025.343926","url":null,"abstract":"<div><h3>Background</h3><div>Lipopolysaccharide (LPS), a bacterial endotoxin prevalent in Gram-negative pathogens (e.g., <em>Escherichia coli</em>), induces severe immune responses linked to endotoxemia and hepatitis. Despite its clinical significance, conventional LPS detection methods (e.g., limulus amebocyte lysate assays) face challenges including operational complexity, high cost, and limited sensitivity. Addressing these limitations necessitates the development of innovative strategies for ultrasensitive LPS quantification.</div></div><div><h3>Results</h3><div>We present an electrochemical biosensor integrating dual-signal amplification: (1) affinity amplification via phenylboronic acid-cis-diol covalent binding on LPS polysaccharide chains, and (2) photocatalytic amplification using perylene diimide (PDI)-mediated atom transfer radical polymerization (Photo-ATRP) under red light (615–650 nm). Thiol-functionalized DNA aptamers enable specific LPS capture, while PDI catalyzes rapid ferrocene monomer polymerization, achieving exponential signal enhancement. The sensor demonstrates exceptional performance: (1) Ultrahigh sensitivity: Detection limit of 0.25 fg/mL. (2) Wide dynamic range: Linear response from 1.0 fg/mL to 0.1 pg/mL. (3) Robust specificity: Minimal interference in human serum matrices.</div></div><div><h3>Significance</h3><div>This work establishes a paradigm for LPS detection through three key advances: (1) Operational simplicity: Eliminates enzymatic/nanomaterial dependencies via metal-free PDI photocatalysis. (2) Translational utility: Serum compatibility supports clinical diagnostics and point-of-care applications. (3) Catalytic innovation: Validates PDI as a high-efficiency photocatalyst for controlled polymer synthesis. The sensor's low-cost fabrication, rapid response (&lt;4.5 h), and femtomolar sensitivity position it as a transformative tool for sepsis monitoring and biomedical research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343926"},"PeriodicalIF":5.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143590206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A washing-less biosensor based on the dual functions of magnetic separation and signal output of magnetic nanoparticles for the rapid and visual detection of enrofloxacin","authors":"Jiangqi Wang ,&nbsp;Yafang Shen ,&nbsp;Guijie Hao ,&nbsp;Xingyue Tang ,&nbsp;Huang Dai ,&nbsp;Haiqi Zhang ,&nbsp;Qingman Yang ,&nbsp;Dongren Zhou","doi":"10.1016/j.aca.2025.343923","DOIUrl":"10.1016/j.aca.2025.343923","url":null,"abstract":"<div><h3>Background</h3><div>There is an urgent need for <em>in-situ</em> detection of antibiotic residues to enhance both food safety and environmental sustainability. Although various biosensing methods have been elaborately designed and achieved extremely high sensitivities, they are always complicated and require sophisticated instruments, making them unsuitable for on-site application. Herein, a simple and washing-less biosensor that took full advantage of magnetic nanoparticles (MNs), <em>i.e</em>., magnetic separation and signal output, was developed for the rapid and sensitive detection of enrofloxacin (ENR) residues.</div></div><div><h3>Results</h3><div>The surface of MNs with diameters of approximately 20 nm (MN₂₀) and 130 nm (MN₁₃₀) were modified with ENR and antibodies to prepare MN₂₀-ENR and immunomagnetic nanoparticles (IMN₁₃₀), respectively. In the absence of ENR, the MN₂₀-ENR analogues were captured on the surface of IMN₁₃₀, forming “MN₂₀-ENR-IMN₁₃₀” complexes, which were larger in volume and could be quickly separated in a magnetic field. While in the presence of ENR, the target ENR competed for the binding sites on IMN₁₃₀ surface, inhibiting the formation of the “MN₂₀-ENR-IMN₁₃₀” structures. Therefore, the separation speed significantly slowed down. Qualitative or semi-quantitative determination of ENR residues could be achieved via visual observation of the color changes, while quantitative detection was also achieved with the aid of a microplate reader or merely a smartphone. The developed method enabled sensitive, selective and rapid detection of ENR with a limit of detection of 0.18 ng mL⁻¹ and a detection time of 25 min.</div></div><div><h3>Significance</h3><div>This method uses MNs for both magnetic separation and signal output, simplifies the detection process and eliminates the use of sophisticated instruments, providing a powerful tool for the rapid and <em>in-situ</em> detection ENR residues in various fields such as food safety and environmental protection.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1352 ","pages":"Article 343923"},"PeriodicalIF":5.7,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}