Analytica Chimica Acta最新文献

{"title":"Dual emission chiral carbon dots as fluorescent probe for fast chiral recognition of tryptophan enantiomers","authors":"Qi Wu ,&nbsp;Yuan Chen ,&nbsp;Yan-Li Wang ,&nbsp;Ji-Ying Song ,&nbsp;Hai-Tao Lv ,&nbsp;Ya-Ming Sun","doi":"10.1016/j.aca.2024.343414","DOIUrl":"10.1016/j.aca.2024.343414","url":null,"abstract":"<div><h3>Background</h3><div>Chirality is an essential property of nature. Chiral recognition is of great significance to life sciences, pharmaceutical industry, food analysis, and so on. Chiral carbon dots (CCDs), as green nanomaterials, have great prospects in chiral sensing. However, CCDs with enantioselectivity for tryptophan (Trp) enantiomers are scarce. Moreover, most chiral sensing platforms depend on the difference of fluorescence intensity at the same emission wavelength to identify enantiomers, it is still a challenge to distinguish enantiomers by the positions of fluorescent emission peaks.</div></div><div><h3>Results</h3><div>Novel CCDs with specific chiral recognition ability for Trp enantiomers are synthesized using <span>l</span>-lysine and <span>l</span>-cysteine as precursors. The CCDs have two fluorescent emission peaks at 390 nm and 450 nm. Interestingly, the fluorescence intensity of CCDs at 390 nm enhances obviously on the addition of L-Trp, while it enhances slightly at 450 nm in the presence of D-Trp. This chiral sensing system not only can identify Trp enantiomers according to fluorescence intensity, but also achieves the distinguishment depending on emission wavelength. The enantioselectivity (I_L/I_D) reaches 4.5 when the concentration of Trp enantiomer is 1 mM. This chiral sensing platform not only can be used for quantitative analysis of D-Trp and L-Trp, but also can be used for determining the enantiomeric excess of racemates. The chiral recognition mechanism is investigated by molecular simulation. It is found that L-Trp has higher binding energy with CCDs.</div></div><div><h3>Significance</h3><div>This work presents a novel kind of CCDs with special chiral recognition performance for Trp enantiomers, and opens the door to identify chiral isomers according to wavelength difference, which has profound significance for the development of chiral sensing platforms, and may provide inspirations for the design of novel CCDs with excellent chiral recognition performance.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343414"},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automatic and integrated detection of nucleic acid by using a dual-mode thermal controlled digital microfluidic chip","authors":"Jiaqi Liao ,&nbsp;Chonghao Li ,&nbsp;Jiajia Liu ,&nbsp;Hao Cheng ,&nbsp;Linmin Li ,&nbsp;Guixi Tang ,&nbsp;Ru Huang ,&nbsp;Yating Lu ,&nbsp;Siyue Chen ,&nbsp;Qian Zhang ,&nbsp;Hao Chen ,&nbsp;Qinyuan Chen ,&nbsp;Hong Chen ,&nbsp;Daoheng Sun","doi":"10.1016/j.aca.2024.343415","DOIUrl":"10.1016/j.aca.2024.343415","url":null,"abstract":"<div><div>The detection of nucleic acids, which typically consists of nucleic acid extraction, reverse transcription and amplification, is a crucial component of molecular diagnostics. However, traditional methods have several limitations, including high reliance on skilled personnel, low degree of automation and lengthy assay times. Additionally, these methods also require separate areas for the different steps to avoid contamination. Though a number of digital microfluidic (DMF) chips have been reported to detect the nucleic acids, but there is a noticeable absence of fully integrated DMF platforms capable of concurrently performing nucleic acid extraction, amplification, and signal detection. Here, a DMF chip combined with a dual-mode thermal control module was developed to realize the automatic and integrated detection of nucleic acids. The dual-mode thermal control module provided the required temperatures (with a range from 4 °C to 95 °C) for all the steps of nucleic acid detection. Pseudoviruses of SARS-CoV-2 and monkeypox were chosen to demonstrate the feasibility of the DMF chip, and all assays were completed within 40 min. The results confirmed that the products extracted under 4 °C exhibited high concentration and integrity, which were comparable to those by traditional methods. And the analytical performance of amplify was comparable to that of traditional methods with a more compact design, shorter time, smaller size and more automatic operation. With the ability of dual-mode thermo control and full automatic operation, nucleic acid detection with the characteristics of sample-in-result-out was realized on the DMF chip, which holds great promise in fields such as in vitro diagnostics and point‐of‐care testing.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343415"},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ratiometric fluorescent paper chip for monitoring the freshness of high protein foods","authors":"Guobin Mao ,&nbsp;Yuan Zeng ,&nbsp;Chunmin Qiu ,&nbsp;Guangmiao Ding ,&nbsp;Leyao Li ,&nbsp;Lixin Ma ,&nbsp;Junbiao Dai ,&nbsp;Wen Yin ,&nbsp;Yingxin Ma","doi":"10.1016/j.aca.2024.343418","DOIUrl":"10.1016/j.aca.2024.343418","url":null,"abstract":"<div><div>Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H₂S) is produced during spoilage-related processes, abnormal H₂S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S²⁻) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S²⁻ increased, the fluorescence intensity of Si/CdTe NPs at λ_em = 488 nm increased, while the fluorescence intensity at λ_em = 620 nm was quenched. The fluorescence intensity ratio (F₆₂₀/F₄₈₈) was negatively linearly correlated to S²⁻ concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H₂S concentration using a novel concept to ensure the freshness and safety of high-protein foods.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343418"},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benzimidazole-based bimodal periodic mesoporous organosilica for thin film microextraction of triazole fungicides in fruiting vegetables prior to gas chromatography-mass spectrometry","authors":"Faezeh Karimi Zandian, Hadiseh Zare Rafie, Habib Bagheri","doi":"10.1016/j.aca.2024.343420","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343420","url":null,"abstract":"<h3>Background</h3>Periodic mesoporous organosilicas (PMOs) are a new class of organic-inorganic hybrid materials with high surface area, narrow pore size distribution, high functional group loading, and tunable functional groups. In contrast to other porous organosilicate materials, PMOs show a uniform distribution of organic groups inside their framework walls. They are synthesized by condensing bis-silylated organic precursors around a surfactant template. Their application severely depends on textural features like pore structure, particle size, porosity and surface area. This study focuses on the application of PMOs in trace determination of pesticides, while the structural features of these intersting materials are strongly considered.<h3>Results</h3>Different PMOs with UVM-7 and SBA-15 type mesostructures were synthesized using benzimidazolium salt as the organic modifier. Then the prepared PMOs were employed in thin film microextraction (TFME) composed from Nylon-6 substrate prior to gas chromatography-mass spectrometry (GC-MS) analysis. Afterwards, a simple method was adapted for detecting trace amounts of six triazole fungicides in extracts of five commonly consumed fruiting vegetables. The prepared bridged benzimidazolium salt organosilica-based sorbents underwent extensive characterization by FTIR, TEM, SEM, EDX, BET and XRD investigations. After obtaining the extraction results for both PMOs, the best one was implemented as the extractive phase in the further experiments. After optimization, limits of detection and quantification ranged from 0.02-0.2 and 0.06-0.7 μg L^-1, respectively. The linearity of calibration plots varies at the range of 0.1-500 μg L^-1 (R² = 0.996). Relative standard deviations (RSDs) were found to be 3.9-14.1%. The relative recoveries (85-115%) show that this approach works appropriately to detect triazole fungicides in real matrixes.<h3>Significance</h3>PMOs framework upon similar structures with azole fungicides provide interaction between sorbent and the desired analytes. For the first time, the superior PMO nanoparticles with high surface area and accessible bimodal pore systems (UVM-7) were evaluated in thin film microextraction of triazole fungicides from 10-fold diluted fruiting vegetable samples. This study proves PMO structure can be engineered by controlling the synthesis condition depending on application requirements. This finding opens a new horizon in preparing structurally specified sorbents for microextraction purposes.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"38 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conjugated Polymer-Boosted Near-infrared Electrochemiluminescence of Organic Dye for Detecting Acetamiprid","authors":"Xin Wen, Ying He, Ruo Yuan, Shihong Chen","doi":"10.1016/j.aca.2024.343417","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343417","url":null,"abstract":"<h3>Background</h3>The near-infrared electrochemiluminescence (NIR-ECL) has excellent penetration and near zero background interference, and has shown unique advantages in clinical medicine and bioimaging. Among various types of NIR-ECL emitters, NIR organic dyes have arouse the concern of researchers due to their adjustable structure and diverse optical properties. However, the currently available NIR dyes usually have inherent self-quenching effect and poor photostability, so their ECL efficiency is low, and it is a great challenge to improve their ECL performance.<h3>Result</h3>Conjugated polymer-boosted NIR-ECL strategy was creatively developed to overcome ECL performance limitations of NIR dyes. IR 783, as one of heptamethine cyanine dyes, was performed a nanoprecipitation in the presence of poly[(9,9-dlhexyfluoren-2,7-dlyl)-co-(anthracen-9,10-dlyl)] (PFAD) to prepare IR polymer nanoparticles (IR PNPs). Due to resonance energy transfer (RET) from PFAD to IR 783 and encapsulation of IR 783 by PFAD, the resulting IR PNPs exhibited a strong and stable NIR-ECL emission with a maximum ECL wavelength of 802 nm under coreactant tripropylamine (TPrA) and H₂O₂ can effectively quench it. IR PNPs coupled proximity ligation assay (PLA)-induced DNA walker to achieve acetamiprid (ACE) analysis. ACE triggered PLA to form bipedal DNA walker, and further release G-rich secondary target (ST). With ST and hemin being captured on IR PNPs modified electrode, hemin/G-quadruplex was assembled to consume H₂O₂, thereby restoring ECL signal for ACE detection with a limit of detection of 4.74 ×10^-15 M.<h3>Significance</h3>This work opens up a new and simple way to boost NIR-ECL of organic dyes, and IR PNPs create a promising NIR-ECL platform for pesticide detection.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"216 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An innovative biosensor utilizing aptamer-gated mesoporous silica nanoparticles for determination of aminoglycoside antibiotics through indirect-lateral flow","authors":"Reza Ghaffari ,&nbsp;Reza Moradi ,&nbsp;NoorMohammad Danesh ,&nbsp;Mohammad Ramezani ,&nbsp;Mona Alibolandi ,&nbsp;Khalil Abnous ,&nbsp;Seyed Mohammad Taghdisi","doi":"10.1016/j.aca.2024.343413","DOIUrl":"10.1016/j.aca.2024.343413","url":null,"abstract":"<div><h3>Background</h3><div>Aminoglycoside antibiotics (AGs) are commonly utilized in both human and veterinary medicine to treat and manage a range of infections. These antibiotics are recognized for their narrow therapeutic window, with an overdose potentially resulting in severe side effects like kidney and ear damage. Hence, the implementation of a quick, precise, and on-the-spot testing method is crucial in clinical settings. In the present investigation, we designed an innovative indirect lateral flow assay (LFA) utilizing aptamers to detect kanamycin, a type of aminoglycoside antibiotics. We prepared mesoporous silica nanoparticles (MSNs) functionalized with amino groups, loaded them with morphine (MOP), and then sealed them with aminoglycoside aptamers (Apt). The complex of gated-MSNs@Apt was tested using the MOP LFA in the presence or absence of kanamycin antibiotics.</div></div><div><h3>Results</h3><div>The indirect LFA system displayed a single colored line in the test line for positive samples, whereas it exhibited double colored lines in both the control line and test line for negative samples. Our custom-designed LFA biosensors demonstrated two linear ranges, from 10 nM to 350 nM and 500 nM–1500 nM, with a limit of detection (LOD) of 5 nM in serum media under optimized conditions. The introduced indirect LFA biosensor was effectively utilized to detect kanamycin, achieving a satisfactory recovery rate of 92.9–109.9 % with RSD of 1.68–7.73 % in serum samples.</div></div><div><h3>Significance</h3><div>In general, the created LFA system offers a portable, straightforward, and affordable approach for point-of-care (POC) identification of kanamycin and other AGs.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343413"},"PeriodicalIF":5.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Static headspace-gas chromatography with mass spectrometry for the assessment of the bioaccumulation of volatile organic compounds associated with microplastics in animal tissues","authors":"Rosa Peñalver,&nbsp;María Dolores Pérez-Álvarez,&nbsp;Natalia Campillo,&nbsp;Natalia Arroyo-Manzanares,&nbsp;Pilar Viñas","doi":"10.1016/j.aca.2024.343412","DOIUrl":"10.1016/j.aca.2024.343412","url":null,"abstract":"<div><h3>Background</h3><div>The excessive increase in the production of plastics worldwide and their mismanagement have turned these materials into a threat to the environment and living organisms. Plastics undergo erosion and degradation processes releasing additives, monomers of their formulation, and even generating one of the most worrying emerging pollutants: microplastics. These contaminants have been found in both aquatic and terrestrial ecosystems, so it is clear the need to develop a methodology to determine the accumulation of these pollutants and their associated compounds in living organisms.</div></div><div><h3>Results</h3><div>This work presents a new combination of static headspace with gas chromatography coupled to mass spectrometry (SHS-GC-MS) for the determination of volatile organic compounds (VOCs) associated with microplastic materials in animal tissue samples. A total of 48 samples were analysed, corresponding to different organs (brain, heart, fat, liver, muscle, lung, kidney) of lamb, pig, rabbit, turkey, chicken, and beef, and 60 VOCs related to the presence of plastics were tentatively identified. Moreover, the identity of 25 VOCs was confirmed and they were also quantified, with contents in the 1.12–920 ng g⁻¹ range. The application of chemometric tools allowed to evaluate the relationship between the type of animal organ and the presence of contaminants associated with microplastics, showing a tendency towards a greater accumulation in kidney and liver. Verification of the origin of the VOCs found was carried out by means of a thermogravimetric analysis - mass spectrometry study.</div></div><div><h3>Significance</h3><div>The developed analytical approach generates useful and valuable information to better understand the exposure pathways of microplastics and their associated compounds, as well as the related migration and accumulation processes may happen in animal organs. This methodology has demonstrated high sensitivity, precision, robustness, and automation, as well as minimal sample handling.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343412"},"PeriodicalIF":5.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electrochemiluminescence sensor for ultrasensitive determination of tyrosine based on ceria nanomaterial as a novel luminophor","authors":"Yaxuan Qian ,&nbsp;Zhihua Wang","doi":"10.1016/j.aca.2024.343388","DOIUrl":"10.1016/j.aca.2024.343388","url":null,"abstract":"<div><h3>Backgroud</h3><div>Recently, optical nanomaterials have attracted much attention in the field of electrochemiluminescence (ECL). Nanostructured ceria possessed unique optical properties, and it's always used for constructing ECL sensor as catalyst for improving sensing performance, while the ECL property of ceria is rarely studied. In fact, it could be the potential candidate for applying in ECL sensors based on size and shape dependency optical property caused by the quantum scale effects. <strong>Therefore, new simple and efficient ceria nanomaterial used as luminophor for constructing the ECL sensor have aroused more research interest and motivation.</strong> (91 words).</div></div><div><h3>Results</h3><div>In the work, three kinds of nanostructured ceria, ceria nano-cube (CeO₂-NC), ceria nano-rods (CeO₂-NR) and ceria nano-particle (CeO₂-NP), have been prepared by hydrothermal method, and CeO₂-NC with the less oxygen vacancy has the highest ECL signal. Moreover, how the shape affects the ECL property was investigated in detail, especially the oxygen vacancy on the surface of the ceria. The less of oxygen vacancy content of CeO₂ nanomaterials is more favorable the ECL reaction. Furthermore, a novel ECL sensor was constructed based on reduced graphene oxide (rGO), Au nano-particle (AuNPs) and CeO₂-NC for Tyrosine (Tyr) determination owing to the electron transform between the luminous of ceria and Tyr resulting in the cathodic ECL intensity quenched correspondingly, and it has possessed excellent sensing performance with a linear range of 0.005–20 nmol/L and the limit of detection (LOD) value of 1.7 fmol/L (141 words)</div></div><div><h3>Significance and novelty</h3><div>The proposed method demonstrated excellent selectivity and ultra-sensitivity toward Tyr, which had been used for the determination of Tyr in human serum successfully, possessing outstanding analytical performance. Therefore, nanostructured ceria is a new category of efficient and promising luminophore, which would open new avenues for the potential application in the field of ECL sensing for clinical diagnosis. (57 words).</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343388"},"PeriodicalIF":5.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel loop-mediated isothermal amplification for ultra-fast diagnosis of Helicobacter pylori in stool samples without nucleic acid extraction","authors":"Jinling Cui ,&nbsp;Anning Tian ,&nbsp;Haoran Wang ,&nbsp;Yanan Yu ,&nbsp;Jingwen Hao ,&nbsp;Lei Wang ,&nbsp;Chao Shi ,&nbsp;Cuiping Ma","doi":"10.1016/j.aca.2024.343384","DOIUrl":"10.1016/j.aca.2024.343384","url":null,"abstract":"<div><h3>Background</h3><div>The gastrointestinal diseases caused by <em>Helicobacter pylori</em> (<em>H. pylori</em>) infection made the accurate detection of <em>H. pylori</em> infection more important. Non-invasive methods, such as molecular diagnostic methods, had become a promising method for detection of <em>H. pylori</em>. Stool samples combined with loop-mediated isothermal amplification (LAMP), showed potential practicability for real-time detection. However, complex nucleic acid extraction steps were required to remove the large numbers of amplification inhibitors in stool samples before LAMP reaction. And the limited number of <em>H. pylori</em> made the detection with long reaction time and low sensitivity. The problems mentioned above were urgently to be solved.</div></div><div><h3>Results</h3><div>In this study, we proposed a strategy for ultra-rapid sensitive detection of <em>H. pylori</em> in stool samples by hydrogel LAMP (hLAMP) without extraction. The hydrogel was combined with stool samples after simple thermal cracking, and amplification spaces were formed in its nanopore structures by nano-localization. The LAMP reaction was accelerated by nano space-localization. Besides, this method based on hLAMP could specifically and sensitively detect as low as 100 CFU/mL <em>H. pylori</em> within 40 min from sampling to result due to good anti-inhibition effect on complex samples of hydrogel. The whole process involved sample simple disposal for 10 min and LAMP reaction for 30 min. Furthermore, the excellent anti-inhibition mechanism of hydrogel was discussed, and the mechanism of hydrogel accelerating LAMP was explored.</div></div><div><h3>Significance</h3><div>This is the first application of that hydrogel and LAMP systematically combined to detect <em>H. pylori</em> in stool samples. The developed method had been verified in actual clinical applications that the accuracy rate reached 88.9 % compared with routine histopathology. And it also provided a potential idea for the diagnosis and prevention of <em>H. pylori</em>.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343384"},"PeriodicalIF":5.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic-based fluorescence enhancement of silica-embedded carbon dots for direct detection and quantification of unamplified HCV RNA in clinical samples","authors":"Hanan Shaat ,&nbsp;Mohamed Sharafeldin ,&nbsp;Amany Mostafa ,&nbsp;Eman H. Ismail ,&nbsp;Mohmed K. Hassan ,&nbsp;Mohamed H. Alkordi ,&nbsp;El-Zeiny M. Ebeid ,&nbsp;Hesham Elghazaly ,&nbsp;Sara H. Agwa ,&nbsp;Sherif M. Shawky","doi":"10.1016/j.aca.2024.343396","DOIUrl":"10.1016/j.aca.2024.343396","url":null,"abstract":"<div><h3>Background</h3><div>Hepatitis C Virus (HCV) is an asymptomatic chronic infection with serious clinical consequences. Timely and sensitive detection of HCV RNA is critical for infection control, treatment response follow up. While current technologies, such as PCR or isothermal amplification-based strategies, are specific, they are expensive, labor intensive, time consuming limiting their use in field and small laboratories.</div></div><div><h3>Results</h3><div>We introduce a novel technology for detecting nucleic acids, as exemplified by HCV RNA, in clinical specimens. This approach utilizes the crosslinked Enhanced Emission (CEE) phenomena upon mixing fluorescent amino functionalized silica-coated nitrogen-doped carbon dots (N-CDs/SiO₂/NH₂) with magnetic extracted unamplified HCV RNA showed a significant and immediate fluorescence enhancement. This method was integrated into a semi-automated 3D printed microfluidic chip, wherein the unamplified RNA was mixed with the N-CDs/SiO₂/NH₂. The assay was also employed on the conventional 96-well plate format. This assay offers high sensitivity with detection limit of 500 IU/ml and 1000 IU/ml for the chip and well plate respectively. The sample-to-result time was &lt;20 min on the chip and is simpler than the amplification-based techniques. Analyzing 141 patient samples yielded sensitivity and specificity of 96.47 % and 98.79 % respectively.</div></div><div><h3>Significance</h3><div>This application of N-CDs/SiO2 as nucleic acid fluorescent probes for the first time, offers a versatile, cost-effective, and universal platform for nucleic acids detection. The developed system can be employed in conventional microwell plate-based detection with minimal modifications to current laboratory setups. Additionally, it was integrated into a 3D-printed microfluidic chip enabling enhancement in specificity, sensitivity and accuracy.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343396"},"PeriodicalIF":5.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}