Analytica Chimica Acta最新文献

{"title":"AuNPs@UiO-66-(COOH)2–NH2/Eu for ratiometric fluorescent and colorimetric sensing of trace inorganic mercury in water samples","authors":"Zifei Wu ,&nbsp;Hao Ding ,&nbsp;Kailai Xu ,&nbsp;Chenghui Li ,&nbsp;Xiandeng Hou","doi":"10.1016/j.aca.2025.344197","DOIUrl":"10.1016/j.aca.2025.344197","url":null,"abstract":"<div><h3>Background</h3><div>due to the toxicity and widespread distribution of inorganic mercury ions (Hg²⁺), it is a crucial task for researchers to develop simple methods for field analysis of water samples for trace Hg²⁺.</div></div><div><h3>Result</h3><div>a colorimetric and ratiometric fluorescent dual-mode assay based on bifunctional UiO–(COOH)₂–NH₂/Eu and gold nanocomplexes (AuNPs@UiO–(COOH)₂–NH₂/Eu) was constructed for the sensitive, selective and potentially on-site determination of Hg²⁺. The incorporation of Hg²⁺ resulted in the formation of gold amalgam, which enhanced peroxidase-like activity of AuNPs@UiO–(COOH)₂–NH₂/Eu, accelerating the oxidization of zymolyte 3,3′,5,5′-tetramethylbenzidine (TMB). Concurrently, the color of the reaction solution turned blue, and the RGB (red, green, and blue) of the solution color changed accordingly. The fluorescence at 618 nm was almost unchanged with the increase of Hg²⁺ concentration, whereas the fluorescence at 460 nm was obviously quenched due to the inner filter effect (IFE). Under UV irradiation, the fluorescence emission of the sensing system changed from blue to red, enabling easy visual detection. The probe showed great linearity over a wide range of 20–1200 nM (colorimetric) and 50–4000 nM (fluorescent), with limits of detection (LODs) of 8 nM and 3 nM, respectively.</div></div><div><h3>Significance</h3><div>the proposed method was successfully applied to the determination of trace Hg²⁺ in tap water and groundwater, while the use of a smartphone enables its simplicity and portability. The results show that the colorimetric and ratiometric fluorescent dual-mode method based on AuNPs@UiO–(COOH)₂–NH₂/Eu can achieve rapid visual determination of Hg²⁺ potentially useful for on-site analysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1363 ","pages":"Article 344197"},"PeriodicalIF":5.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode nanosensor for sensitive detection of methotrexate based on fluorescence technology and deep learning algorithms","authors":"Chenyue Kang ,&nbsp;Yueying Li ,&nbsp;Yan Zhang ,&nbsp;Ying Zhou ,&nbsp;Caihong Zhang ,&nbsp;Shaomin Shuang ,&nbsp;Guomei Zhang","doi":"10.1016/j.aca.2025.344190","DOIUrl":"10.1016/j.aca.2025.344190","url":null,"abstract":"<div><h3>Background</h3><div>Methotrexate (MTX) in the body can result in severe, potentially life-threatening side effects. As a result, it is imperative to establish a dependable, precise, and specific method for detecting MTX. However, conventional sensors often suffer from limitations such as low sensitivity, prolonged response times, and insufficient accuracy.</div></div><div><h3>Result</h3><div>To solve this problem, we propose a novel nanoscale double-emission ratiometric fluorescent sensor (N-CDs@mSiO₂-SH@AuAg NCs) that leverages the unique properties of nanomaterials for enhanced detection performance. This sensor integrates sulfhydryl-modified mesoporous silica-encapsulated nitrogen-doped carbon dots (N-CDs@mSiO₂-SH) with bimetallic gold-silver nanoclusters (GSH@AuAg NCs), creating a nanoscale platform with dual emission peaks at 460 nm and 600 nm under 370 nm excitation. The introduction of MTX triggers a distinct fluorescence response: the intensity increases at 460 nm while decreases at 600 nm, accompanied by a visible color change from orange-red to blue. This phenomenon is attributed to the interaction between the carboxyl groups in MTX and the GSH@AuAg NCs, which disrupts the fluorescence resonance energy transfer (FRET) process between the N-CDs@mSiO₂-SH and GSH@AuAg NCs.</div></div><div><h3>Significance</h3><div>The sensor has an impressive wide linear range of 5.00–500.00 μM and a low detection limit of 1.75 μM. In addition, a deep learning algorithm is utilized to predict concentration levels in real-time images, enabling rapid and accurate monitoring of MTX levels in complex detection scenarios.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1362 ","pages":"Article 344190"},"PeriodicalIF":5.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the hyphenation of ion chromatography with optical emission spectrometry for trace analysis of oxyhalide bromine and chlorine species","authors":"Philipp C. Gillemot ,&nbsp;Axel Kretschmer ,&nbsp;Roman Grimmig ,&nbsp;Ursula E.A. Fittschen ,&nbsp;Steffen Witzleben","doi":"10.1016/j.aca.2025.344185","DOIUrl":"10.1016/j.aca.2025.344185","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;The increased application of oxidative water treatment is associated with the presence of halogenated disinfection by-products (DBPs) in aqueous systems. Due to their hazard potential, permanent measurements of selected analytes are required to monitor their compliance with regulatory limits and guidelines. However, the simultaneous acquisition of alarming inorganic oxyhalide species by conventional ion chromatography (IC) is often impeded by co-elution of interfering analyte or matrix components, especially when DBPs of multiple halogens are present in solution. This necessitates a complementary, orthogonal detection setup that allows for an element-specific analysis.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The present work evaluates the hyphenation of IC with optical emission spectrometry (ICP-OES) for the simultaneous quantification of five prominent bromine and chlorine species, namely &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msup&gt;&lt;mtext&gt;Br&lt;/mtext&gt;&lt;mo&gt;–&lt;/mo&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msup&gt;&lt;msub&gt;&lt;mtext&gt;BrO&lt;/mtext&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;–&lt;/mo&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msup&gt;&lt;mtext&gt;Cl&lt;/mtext&gt;&lt;mo&gt;–&lt;/mo&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msup&gt;&lt;msub&gt;&lt;mtext&gt;ClO&lt;/mtext&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;–&lt;/mo&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msup&gt;&lt;msub&gt;&lt;mtext&gt;ClO&lt;/mtext&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;–&lt;/mo&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, at trace concentrations ranging from 2.5 to 100 μmol L&lt;sup&gt;−1&lt;/sup&gt; for Br species and 5–100 μmol L&lt;sup&gt;−1&lt;/sup&gt; for Cl species. It is shown that ICP-OES detection in the vacuum UV region below 170 nm leads to higher sensitivity for Br species, with &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msup&gt;&lt;msub&gt;&lt;mtext&gt;BrO&lt;/mtext&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;–&lt;/mo&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; showing a significantly (&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;p&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;5&lt;/mn&gt;&lt;mspace&gt;&lt;/mspace&gt;&lt;mo&gt;·&lt;/mo&gt;&lt;mspace&gt;&lt;/mspace&gt;&lt;msup&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;mrow&gt;&lt;mo&gt;‐&lt;/mo&gt;&lt;mn&gt;9&lt;/mn&gt;&lt;/mrow&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;) stronger signal response than &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msup&gt;&lt;mtext&gt;Br&lt;/mtext&gt;&lt;mo&gt;–&lt;/mo&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;. The developed method enables the selective quantification of co-eluting (oxy-)halides and shows excellent linearity (R&lt;sup&gt;2&lt;/sup&gt; &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mo&gt;≥&lt;/mo&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; 0.999) and coefficients of variation of the procedure V&lt;sub&gt;x0&lt;/sub&gt; &lt; 5 % for all analytes. Limits of detection below 100 μg L&lt;sup&gt;−1&lt;/sup&gt; were achieved, in conjunction with mean recovery rates of 98 &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mo&gt;±&lt;/mo&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; 4 % in challenging matrices.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Significance&lt;/h3&gt;&lt;div&gt;For the first time, Br and Cl speciation was successfully achieved by using IC-ICP-OES hyphenation. The results highlight the robustness and applicability of the developed method exemplarily for oxyhalide speciation, clearly differentiating species-specific behavior of halogens in an emission spectrometer. This hyphenation technique generally enables solving complex separation problems by simultaneously evaluating diff","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1362 ","pages":"Article 344185"},"PeriodicalIF":5.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesoporous Ce-UiO-66 with enhanced laccase-like activity for fast detection of epinephrine and specific identification of tetracycline antibiotics","authors":"Xuan Xu,&nbsp;Mengmeng Liu,&nbsp;Cheng Yao","doi":"10.1016/j.aca.2025.344174","DOIUrl":"10.1016/j.aca.2025.344174","url":null,"abstract":"<div><div>The challenges associated with the practical application of natural laccase, such as poor reusability and intrinsic fragility, have driven the exploration of alternative solutions. In particular, cerium-based nanozymes emerge as promising candidates due to their high O₂ mobility and inherent redox reactivity. However, there is a need for more in-depth and systematic research to enhance their catalytic activity and expand their application areas. Herein, a mesoporous cerium-based MOF material (MPUiO-66(Ce)) was synthesized with the cooperative assembly of amphiphilic molecular template and framework precursors. Compared with natural laccase, MPUiO-66(Ce) exhibited ∼4.3-fold higher catalytic efficiency, along with enhanced stability under harsh conditions. Besides, the catalytic efficiency of MPUiO-66(Ce) was ∼4.0-fold higher than that of non-porous Ce-UiO-66 (NPUiO-66(Ce)). This enhancement can be attributed to its mesoporous structure, which acted as bionic pockets and provided favorable conditions for the effective diffusion and capture of substrates. Based on the above advantages, MPUiO-66(Ce) was employed for colorimetric sensing epinephrine (EP) and tetracycline (TC), respectively. EP can be detected within 5 min, resulting in a low detection limit of 400 nM and a broad linear range of 10–220 μM. Besides, the MPUiO-66(Ce)-based colourimetric platform could discriminate TC from other antibiotics, showing a wide linear range (20–250 mΜ) and a low detection limit of 810 nM. Furthermore, this sensing platform can accurately identify EP in serum, as well as TC in tap water, river water and soil. This work provided a new approach for designing cerium-based nanoenzymes with improved catalytic activity and highlighted their potential applications in the fields of life analysis and environmental monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1362 ","pages":"Article 344174"},"PeriodicalIF":5.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143940532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equidistant regions of interest - multivariate curve resolution for clean spectra from plant-metabolomics by gas chromatography with high resolution mass spectrometry","authors":"Zhuang Gao ,&nbsp;Xianghui Yao ,&nbsp;Yimin He ,&nbsp;Shuyi Yu ,&nbsp;Min He","doi":"10.1016/j.aca.2025.344171","DOIUrl":"10.1016/j.aca.2025.344171","url":null,"abstract":"<div><h3>Background</h3><div>The application of Gas Chromatography coupled with Orbitrap High-Resolution Mass Spectrometry (GC-Orbitrap HRMS) in plant metabolomics presents challenges including substantial data storage requirements, system complexity, and particularly the prevalence of co-eluted peaks in elution profiles. These limitations necessitate the integration of chemometric smart tools to extract interpretable mass spectra from complex chromatographic fingerprints. However, the direct applicability of multivariate resolution algorithms to GC-Orbitrap HRMS datasets remains limited. The existing deconvolution platforms exhibit operational constraints such as insensitivity toward embedded peaks and prerequisite data pretreatment.</div></div><div><h3>Results</h3><div>Using <em>Cyperus rotundus</em> and <em>Curcumae Radix</em> as model medicinal plants, this study developed an equidistant Region of Interest (eROI) strategy to enhance metabolite annotation reliability. The eROI method systematically converts raw datasets into structured matrices with standardized dimensions, effectively preserving <em>m/z</em> reading for subsequent analytical phases. We implemented scenario-specific combinations of eROI with three multivariate curve resolution (MCR) techniques to isolate pure component spectra from co-eluted chromatographic features. Comprehensive metabolite annotation was achieved through systematic spectral interpretation of fragmentation patterns, supplemented by predictive mass spectral analysis when database matching proved inconclusive. Detailed validation using volatile-metabolomics fingerprints from both botanical species accompanies each methodological stage.</div></div><div><h3>Significance</h3><div>Our work establishes a novel data reduction framework for GC-HRMS applications, enabling robust multi-modal chromatographic deconvolution. The integrated eROI-MCR methodology provides a validated solution for obtaining reliable qualitative and quantitative results in non-targeted plant-metabolomics studies.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1362 ","pages":"Article 344171"},"PeriodicalIF":5.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143940507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular uptake of polystyrene nanoplastics with surface Functionalization: An AIE-based quantitative approach","authors":"Long Zhang ,&nbsp;Manyu Xue ,&nbsp;Qi Xin ,&nbsp;Jie Tang ,&nbsp;Yin Liu ,&nbsp;Zhengyang Bian ,&nbsp;Xiaoping Zheng","doi":"10.1016/j.aca.2025.344184","DOIUrl":"10.1016/j.aca.2025.344184","url":null,"abstract":"<div><div>The increasing prevalence of nanoplastics (NPs) in the environment has raised significant concerns about their potential for bioaccumulation and toxicity. However, most toxicity studies currently focus on pristine polystyrene NPs (PSNPs), overlooking the impact of environmental weathering on their surface chemistry and interactions with biological systems. This study adopts an aggregation-induced emission (AIE)-based approach to quantify how surface functionalization affects cellular uptake, a critical step in assessing NPs toxicity. We synthesized carboxyl (-COOH) and amino (-NH₂) functionalized PSNPs through emulsion polymerization, incorporating an AIE fluorescent label to enable precise quantification and to distinguish the NPs from endogenous biomolecules. Using mouse macrophages (RAW264.7) as a model, we demonstrated that surface carboxylation significantly enhanced cellular uptake compared to the original NPs. This enhanced uptake effect is likely due to the increased interaction between the functionalized NPs and the cell surface receptors. Quantitative fluorescence analysis and flow cytometry revealed that PSNP-COOH exhibited the highest uptake and the most pronounced effects on cell toxicity. This AIE-based approach offers a more environmentally relevant model for evaluating NPs toxicity, understanding the importance of considering surface modifications when assessing the biological effects of NP exposure.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1362 ","pages":"Article 344184"},"PeriodicalIF":5.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerebral open flow microperfusion (cOFM)-HILIC-HRMS platform for in vivo and in situ monitoring of tumor microenvironment in glioblastoma","authors":"Fernanda Monedeiro,&nbsp;Denise Schimek,&nbsp;Eva-Maria Prugger,&nbsp;Thomas Altendorfer-Kroath,&nbsp;Elmar Zügner,&nbsp;Christoph Magnes","doi":"10.1016/j.aca.2025.344183","DOIUrl":"10.1016/j.aca.2025.344183","url":null,"abstract":"<div><h3>Background</h3><div>Glioblastoma exhibits a very poor prognosis and profound metabolic alterations associated to its aggressiveness. Examining tumor metabolic states is crucial for identifying potential therapeutic targets, enabling personalized medicine applications. However, direct access to the functional glioblastoma tumor microenvironment (TME) <em>in vivo</em> is highly challenging, particularly due to the tumor location and risk of blood-brain-barrier disruption. Here, we developed a novel research platform combining the innovative sampling technology cerebral open flow microperfusion (cOFM) and HILIC-HRMS-based metabolomics to comprehensively characterize the local metabolic phenotype of human glioblastoma in xenograft animal model.</div></div><div><h3>Results</h3><div>Interstitial fluid (ISF) collected directly from the functional inner TME without trauma generation using cOFM, plasma (PL) and cerebrospinal fluid (CSF) were analyzed. The proposed method enabled consistent measurements, detecting nearly 400 metabolites with adequate analytical quality in the study samples, of which 281 were found in brain ISF. ISF presented the highest number of discriminant metabolites, with over 30 % of the metabolome found to be altered in tumors compared to controls (p &lt; 0.05). Fewer significant metabolic changes were assessed in CSF. Correlation analysis of ISF and PL indicated distinct metabolic fluxes through lipid metabolism in these two compartments. Pathway enrichment analysis indicated significant alterations in pathways related to mechanisms contributing to glioblastoma progression. Evaluation of ISF samples revealed that glioblastoma metabolic reprogramming may involve altered activity of ABC transporters, fatty acid oxidation, enhanced metabolism of central carbon, arginine, proline, and amino acid derivatives engaged in redox homeostasis. Evaluation of PL samples indicated a pronounced contribution of systemic lipid-mediated signaling.</div></div><div><h3>Significance</h3><div>ISF presented metabolic features and pathway activities from functional unaffected tumor, portraying metabolic traits of human glioblastoma tissue and corroborating the translational potential of this method. The novel cOFM-HILIC-HRMS research platform for <em>in vivo</em>/<em>in situ</em> monitoring of TME in glioblastoma can offer a promising and standardized tool for neuro-oncological research in the field of drug resistance and therapy development.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1363 ","pages":"Article 344183"},"PeriodicalIF":5.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and application of a NIR benzothianone-based ratiometric fluorescent probe for visual detection of Hg2+ in biological systems and water samples","authors":"Huan Zhang,&nbsp;Haoyu Wu,&nbsp;Limeng Cong,&nbsp;Aojie Dai,&nbsp;Ning Qu,&nbsp;Shuai Guo,&nbsp;Songhua Zhu,&nbsp;Xiangbo Gou,&nbsp;Youlai Zhang","doi":"10.1016/j.aca.2025.344176","DOIUrl":"10.1016/j.aca.2025.344176","url":null,"abstract":"<div><h3>Background</h3><div>Mercury ions, a toxic heavy metal, pose a significant threat to environmental health, leading to pollution and accumulation in living organisms.</div></div><div><h3>Results</h3><div>In this study, we have developed a novel ratiometric near-infrared fluorescent probe for the detection of Hg²⁺. The probe <strong>TM-ND-Hg</strong> demonstrated a remarkable Stokes shift (358 nm), a rapid response time (within 1 min), excellent selectivity, stability, and ratiometric fluorescence changes from green to red. Moreover, the probe enabled the portable detection of Hg²⁺ through color recognition software, which can discern color alterations.</div></div><div><h3>Significance</h3><div>The probe has been successfully applied to monitor Hg²⁺ in living A549 cells, zebrafish, and real water samples, showcasing its potential for environmental and biological monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1362 ","pages":"Article 344176"},"PeriodicalIF":5.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143940510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-house validation of a droplet digital PCR system using a multifactorial experimental design","authors":"Christopher Weidner ,&nbsp;Sophia Edelmann ,&nbsp;Emilie Dagand ,&nbsp;Kathrin Lieske ,&nbsp;Jörn Lämke ,&nbsp;Bertrand Colson ,&nbsp;Steffen Uhlig ,&nbsp;Joachim Mankertz","doi":"10.1016/j.aca.2025.344172","DOIUrl":"10.1016/j.aca.2025.344172","url":null,"abstract":"<div><h3>Background</h3><div>Digital PCR (dPCR, digital polymerase chain reaction) has become an important technology for quantification of nucleic acids and its application in food and feed safety analysis is increasing. International standards such as ISO/IEC 17025 or ISO/IEC 15198 require an appropriate validation of the methods. In this study, a specific approach for the validation and statistical modelling of dPCR systems was developed and applied to the Bio-Rad QX200 Droplet dPCR (ddPCR) system. This approach uses a factorial experimental design and the underlying statistical model reflects the Poisson process governing the measurement mechanism.</div></div><div><h3>Results</h3><div>We show that most of the experimental factors tested, such as the operator, the primer/probe system and the addition of restriction enzymes, have no relevant effect on the quantification of DNA copy numbers, confirming the robustness of the system. However, the choice of the ddPCR master mix and the droplet volume used to calculate DNA copy concentrations are critical factors. Only with the “Supermix for Probes (no dUTP)” was it possible to confirm the accuracy of the ddPCR system over the entire working range.</div></div><div><h3>Significance</h3><div>High precision, sensitivity, uniformity and robustness of the Bio-Rad QX200 ddPCR system were demonstrated. In addition, we present procedures how to increase the number of stabilised droplets leading to improved acceptance for statistical calculations. The new concept for the in-house validation and modelling of digital PCR systems is transferable to other digital PCR technologies.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1362 ","pages":"Article 344172"},"PeriodicalIF":5.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143933105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vial polycaprolactone thin film for sampling and microextraction of sexual hormones in environmental waters and wastewaters followed by HPLC-MS/MS","authors":"Francesca Merlo ,&nbsp;Vincenzo Cerviani ,&nbsp;Clàudia Fontàs ,&nbsp;Andrea Speltini ,&nbsp;Antonella Profumo ,&nbsp;Enriqueta Anticò","doi":"10.1016/j.aca.2025.344164","DOIUrl":"10.1016/j.aca.2025.344164","url":null,"abstract":"<div><h3>Background</h3><div>The sustainability of sample treatments is a hot topic that can be addressed focusing on miniaturization of techniques and design of more efficient and sustainable sorbents. In such scenario, in-vial microextraction is increasingly reported as a reliable alternative to other solid-phase extraction setups, as integrates sampling, adsorption and desorption in a single device before instrumental analysis. Polymeric thin films have proven to be a versatile and efficient material for microextraction processes, but more attention has to be paid to the preparation steps both in terms of nature of polymer and possible co-extractant, and of solvents applied during the solubilization process.</div></div><div><h3>Results</h3><div>An in vial-microextraction device for sex hormones was fabricated using as the extractant a thin film made of a biodegradable polymer (polycaprolactone, PCL) directly formed on the bottom wall of a glass vial. The greenness assessment of the film preparation results in the selection of an environmentally friendly solvent (methyl-tetrahydrofuran) for PCL solubilization. After evaluation of key parameters (contact time, desorption solvent and conditions), efficient adsorption-desorption cycle is completed in 30 min. High simplicity, low sample manipulation (two steps), quantitative adsorption and recovery (EE% &gt; 70; R% &gt; 60 for most analytes, respectively) determined by HPLC- MS/MS greatly improve sample throughput and the greenness of the method (0.66 by AGREEprep and 7.58 by SPMS). The method was successfully validated in actual water samples, showing low limits of detection (2–35 ng L⁻¹), satisfactory linearity (0.5–50 μg L⁻¹), and limited matrix effects (&lt;10 % for river and &lt;25 % for wastewater).</div></div><div><h3>Significance</h3><div>This polycaprolactone film proved to be a biodegradable and sustainable sorbent towards sex hormones. Its applicability in the in-vial thin film microextraction ensures the whole sample treatment in the same vial with high throughput, low energy and solvent consumption, keeping unchanged performance for multiple cycles. The method combines a green sample treatment with accurate detection and high sensitivity by HPLC-MS/MS, representing significant progress in miniaturized devices for environmental scope.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1362 ","pages":"Article 344164"},"PeriodicalIF":5.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143940508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}